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At least 253 records · Page 14

Simulating metagenomic stable isotope probing datasets with MetaSIPSim

DNA-stable isotope probing (DNA-SIP) links microorganisms to their in-situ function in diverse environmental samples. Combining DNA-SIP and metagenomics (metagenomic-SIP) allows us to link genomes from complex communities to their specific functions and improves the assembly and binning of these targeted genomes. However, empirical development of metagenomic-SIP methods is hindered by the complexity and cost of these studies. We developed a toolkit, ‘MetaSIPSim,’ to simulate sequencing read libraries for metagenomic-SIP experiments. MetaSIPSim is intended to generate datasets for method development and testing. To this end, we used MetaSIPSim generated data to demonstrate the advantages of metagenomic-SIP over a conventional shotgun metagenomic sequencing experiment. Through simulation we show that metagenomic-SIP improves the assembly and binning of isotopically labeled genomes relative to a conventional metagenomic approach. Improvements were dependent on experimental parameters and on sequencing depth. Community level G + C content impacted the assembly of labeled genomes and subsequent binning, where high community G + C generally reduced the benefits of metagenomic-SIP. Furthermore, when a high proportion of the community is isotopically labeled, the benefits of metagenomic-SIP decline. Finally, the choice of gradient fractions to sequence greatly influences method performance. Metagenomic-SIP is a valuable method for recovering isotopically labeled genomes from complex communities. We show that metagenomic-SIP performance depends on optimization of experimental parameters. MetaSIPSim allows for simulation of metagenomic-SIP datasets which facilitates the optimization and development of metagenomic-SIP experiments and analytical approaches for dealing with these data.

59 BASIC BIOLOGICAL SCIENCES↗

Rossi-alpha Uncertainty Quantification by Analytic, Bootstrap, and Sample Methods to Inform Fitting Best Practices

The prompt neutron period (the negative reciprocal of the prompt neutron decay constant) can be estimated using the Rossi-alpha technique that is predicated on fitting Rossi alpha histograms and of interest in nuclear criticality safety and nonproliferation. The histograms are traditionally fit with a one-exponential model; however, recent work has proposed a two-exponential model to account for reflector induced phenomenon. Until recently, the uncertainty quantification for either model was inadequate (inaccurate and demanded large measurement times). Measurement uncertainty quantification by sample and analytic methods was developed and validated in Ref. The purpose of this transaction is to (i) validate a new bootstrap method by comparing bin-by-bin error bar estimates and (ii) demonstrate how to choose bin widths and reset times to optimize precision and accuracy.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Introducing a New Spitzer Master BLISS Map to Remove the Instrument Systematic Phase-curve-parameter Degeneracy, as Demonstrated by a Reanalysis of the 4.5 μm WASP-43b Phase Curve

While Spitzer Infrared Array Camera (IRAC) systematics are generally well understood, each data set can provide its own challenges that continue to teach us about the underlying functional form of these systematics. Multiple groups have analyzed the phase curves of WASP-43b with varying detrending techniques, each obtaining different results. In this work, we take another look at WASP-43b while exploring the degenerate relation between Bilinearly Interpolated Subpixel Sensitivity (BLISS) mapping, point response function (PRF)–FWHM detrending, and phase curve parameters. We find that there is a strong correlation between the detrending parameters in the two models, and best-fit phase curve amplitudes vary strongly when the data are temporally binned. To remove this degeneracy, we present a new Gaussian centroided intrapixel sensitivity map (hereafter fixed sensitivity map), generated using 3,712,830 exposures spanning 5 yr, for a variety of aperture sizes at 4.5 μm. We find evidence for time variability in the sensitivity at 3.6 μm and do not generate a visit-independent map for this channel. With the fixed 4.5 μm intrapixel sensitivity map, the best fits for WASP-43b no longer vary strongly with bin size and PRF–FWHM detrending is no longer required to remove correlated noise. For data sets that do not fall completely within the sweet spot, temporal binning should not be used in the analysis of Spitzer phase curves. We confirm nightside emission for WASP-43b with a disk-integrated nightside temperature of 806 ± 48 K at 4.5 μm. The 4.5 μm maps are available at github.com/kevin218/POET.

79 ASTRONOMY AND ASTROPHYSICS↗

Transverse Vector Decomposition Method for Analytical Inversion of Exoplanet Transit Spectra

Abstract We develop a new method for analytical inversion of binned exoplanet transit spectra and for retrieval of planet parameters. The method has a geometrical interpretation and treats each observed spectrum as a single vector r → in the multidimensional spectral space of observed bin values. We decompose the observed r → into two orthogonal components: a wavelength-independent component r → ∥ corresponding to the spectral mean across all observed bins, and a transverse component r → ⊥ that is wavelength dependent and contains the relevant information about the atmospheric chemistry. The method allows us to extract, without any prior assumptions or additional information, the relative mass (or volume) mixing ratios of the absorbers in the atmosphere, the scale height to stellar radius ratio, H / R S , and the atmospheric temperature. The method is illustrated and validated with several examples of increasing complexity.

79 ASTRONOMY AND ASTROPHYSICS↗

Close Major-merger Pairs at z = 0: Bulge-to-total Ratio and Star Formation Enhancement

We present a study of the bulge-to-total ratio (B/T) of a Ks-band-selected sample of 88 close major-merger pairs of galaxies (H-KPAIR), based on two-dimensional decompositions of SDSS r-band images with galfit. We investigate the dependence of the interaction-induced specific star formation rate enhancement (sSFR enh ) on the B/T ratio, and the effects of this dependence on the differences between star-forming galaxies (SFGs) in spiral+spiral (S+S) and spiral+elliptical (S+E) pairs. Of all the 132 spiral galaxies in H-KPAIR, the 44 in S+E pairs show higher B/T than those in the 44 S+S pairs, with means of B/T = 0.35 ± 0.05 and B/T = 0.26 ± 0.03, respectively. Furthermore, there is a strong negative dependence of sSFR enh on the B/T ratio, and only paired SFGs with B/T < 0.3 show significant (>5σ) enhancement. Paired SFGs in S+S pairs show a similar trend, and many disky SFGs (B/T < 0.1) in S+S pairs have strong sSFR enhancements (sSFR enh > 0.7 dex). For SFGs in S+E pairs, the sSFR has no clear B/T dependence, nor any significant enhancement in any B/T bin. Disky SFGs in S+S pairs show significant (>4σ) enhancement in molecular gas content (${M}_{{{\rm{H}}}_{2}}/{M}_{\mathrm{star}}$), while SFGs in S+E pairs have no such enhancement in any B/T bin. No significant enhancement of the total gas content (Mgas/Mstar) is found in any B/T bin for paired galaxies. The star formation efficiency of either the total gas (SFE gas = SFR/M gas ) or molecular gas (${\mathrm{SFE}}_{{{\rm{H}}}_{2}}=\mathrm{SFR}/{M}_{{{\rm{H}}}_{2}}$) does not depend on the B/T ratio. The only significant (> 4σ) SFE enhancement found for paired SFGs is the SFE gas for disky SFGs in S+S pairs.

79 ASTRONOMY AND ASTROPHYSICS↗

Warm-phase microphysical evolution in large-eddy simulations of tropical cumulus congestus: evaluating drop size distribution evolution using polarimetry retrievals, in situ measurements, and a thermal-based framework

Owing to uncertainties in convective microphysics processes, improving parameterizations in Earth system models (ESMs) can benefit from observationally constrained cases suitable for scaling between cloud-resolving models and ESMs. We propose a benchmark large-eddy simulation (LES) cumulus congestus case study from the NASA Cloud, Aerosol, and Monsoon Processes Philippines Experiment (CAMP 2 Ex) for evaluating and improving ESMs in single-column model (SCM) mode. We seek observational constraints using novel polarimetric retrievals and in situ cloud microphysics measurements. Simulations using bulk and bin microphysics initialized with observed aerosol profiles are compared to cloud-top retrievals of cloud droplet effective radius (R eff ), effective variance (ν eff ), and number concentration (N d ) from the airborne Research Scanning Polarimeter (RSP). Both schemes reproduce characteristics of cloud-top N d and R eff that increase and decrease with altitude, respectively. Cloud-top N d is low-biased relative to RSP retrievals in both schemes, potentially due to limitations in both simulations and retrieval assumptions. Cloud-top R eff is low-biased in the bulk scheme but reasonably reproduced by the bin scheme. Profiles of N d and R eff are sensitive to the collision–coalescence process and the vertical variation in aerosol size distribution. Comparison of simulated and in situ droplet size distributions (DSDs) shows that, to first order, integrated moments are always sensitive to sizes < ~ 30 µm and can also be sensitive to larger sizes if the DSDs are sufficiently broad, with implications for the assumed maximum observed size retrieved by the RSP. The bin scheme captures the observed extended tail of the DSD, while the bulk scheme is unable to due to parametric constraints. Differences in expected relationships between in situ measurements of cloud cores and cloud-top retrievals by RSP demonstrate difficulty in constraining well the case presented herein. Finally, a thermal-tracking framework demonstrates that the dilution of N d throughout a thermal's lifetime is heavily determined by collision–coalescence and the height-varying aerosol distribution and that, in the absence of these, the impact of entrainment on diluting N d is largely offset by secondary activation. Implications for evaluating warm-phase convective microphysics schemes in ESMs and translating results for use on global, space-based polarimetry platforms are discussed.

Stanford, McKenna Wallace [Columbia Univ., New Yor↗

NGEE Arctic CO2, CH4 and Energy Eddy-Covariance (EC) Flux Tower Auxiliary Measurements, Utqiagvik (Barrow), Alaska, 2012 - 2019

Measurements began in September 2012 and are generally made throughout the snow-free growing season starting from April (pre-thawing) until November (freeze-up) when the instrumentation is taken down for maintenance and instrument calibration. CO2, CH4 and energy fluxes using the Eddy Covariance (EC) technique (Baldocchi 2003) and meteorological measurements are reported as 30-minute averages. The tower site is registered with AmeriFlux as US-NGB. See http://ameriflux.lbl.gov/sites/siteinfo/US-NGB for more information. Reported data include: (1) additional/extra meteorological and eddy covariance CO2, CH4 and energy flux data, (2) binned spectra for the three wind components, the sonic temperature and gas densities together with the binned cospectra for covariances of w (vertical wind component) and gas densities, (3) binned ogives (cumulative (co)spectra) for the three wind components, the sonic temperature and gas densities, and (4) tower site footprint matrices. Meteorological and eddy covariance CO2, CH4 and energy flux data (AmeriFlux BASE data product) are available from AmeriFlux https://doi.org/10.17190/AMF/1436326. The tower site is adjacent to the NGEE Arctic Tram system with several complementary time-series measurements https://doi.org/10.5440/1558782.This dataset supersedes the previously released dataset "Eddy-Covariance and auxiliary measurements, NGEE-Barrow, 2012-2013" DOI:10.5440/1124200 as this new product includes data for the longer time period processed using consistent methods for the entire record. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) near Utqiagvik (Barrow), Alaska and 2) multiple areas in the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

NGEE Arctic CO2, CH4 and Energy Eddy-Covariance (EC) Flux Tower Auxiliary Measurements, Council Road Mile Marker 71, Seward Peninsula, Alaska, 2017 - 2019

Measurements began in July 2017 and are made year-round (2017 seasonal only) on the tussock tundra with discontinuous permafrost at the Council Road Site on the Seward Peninsula. CO2, CH4 and energy fluxes using the Eddy Covariance (EC) technique (Baldocchi 2003) and meteorological measurements are reported as 30-minute averages. The tower site is registered with AmeriFlux as US-NGC. See http://ameriflux.lbl.gov/sites/siteinfo/US-NGC for more information. Reported data include: (1) additional/extra meteorological and eddy covariance CO2, CH4 and energy flux data, (2) binned spectra for the three wind components, the sonic temperature and gas densities together with the binned cospectra for covariances of w (vertical wind component) and gas densities, (3) binned ogives (cumulative (co)spectra) for the three wind components, the sonic temperature and gas densities, and (4) tower site footprint matrices. The majority of the files are *.csv with paired visualization footprint files as *.jpg. Meteorological and eddy covariance CO2, CH4 and energy flux data (AmeriFlux BASE data product) are available from AmeriFlux BASE US-NGC NGEE Arctic Council (Torn, 2020) https://doi.org/10.17190/AMF/1634883. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) near Utqiagvik (Barrow), Alaska and 2) multiple areas in the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Correlation analysis of X-ray emission from Cygnus X-1

Normalized autocorrelation and cross-correlation coefficients have been calculated for the X-ray emission of Cygnus X-1 in two energy bins, 2.1-5.1 keV and 5.1-12 keV. The analysis shows a strong correlation between the pulsations in the two energy bins, with the low-energy pulsations lagging behind the high-energy ones, or alternatively a shorter duration of the pulse trains in the high-energy bin. The power spectral density derived from the autocorrelation functions indicates a larger contribution of the high-frequency components for the higher-energy X-rays.

Brinkman, A. C.↗

Solar radio burst and in situ determination of interplanetary electron density

A few interplanetary electron density scales which were derived from the analysis of interplanetary solar radio burst are discussed and compared to a model derived from 1974 to 1980 Helios 1 and 2 in situ density observations made in the 0.3 to 1.0 AU range. The Helios densities were normalized to 1976 with the aid of IMP and ISEE data at 1 AU, and were then sorted into 0.1 AU bins and logarithmically averaged within each bin. The best fit to these 1976-normalized, bin averages is N(R(AU)) = 6.1 R(-2.10)/cu cm. This model is in rather good agreement with the solar burst determination if the radiation is assumed to be on the second harmonic of the plasma frequency. This analysis also suggests that the radio emissions tend to be produced in regions denser than the average where the density gradient decreases faster with distance than the observed R(-2.10).

Bougeret, J. L.↗

Solar radio burst and in situ determination of interplanetary electron density

A few interplanetary electron density scales which were derived from the analysis of interplanetary solar radio burst are discussed and compared to a model derived from 1974 to 1980 Helios 1 and 2 in situ density observations made in the 0.3 to 1.0 AU range. The Helios densities were normalized to 1976 with the aid of IMP and ISEE data at 1 AU, and were then sorted into 0.1 AU bins and logarithmically averaged within each bin. The best fit to these 1976-normalized, bin averages is in N(R(AU)) = 6.1 R(-2.10)/cu cm. This model is in rather good agreement with the solar burst determination if the radiation is assumed to be on the second harmonic of the plasma frequency. This analysis also suggests that the radio emissions tend to be produced in regions denser than the average where the density gradient decreases faster with distance than the observed R(-2.10). Previously announced in STAR as N83-35989

King, J. H.↗

Automated Conduit Unloading

Large, cumbersome pipes removed from trailer by one operator. Swiveltruck trailer carries conduit and unloads it. Vertical bins interconnected by web belts that elevate conduit sections for delivery by gravity to unloading point. Trailer loaded with slurry-pipe sections 6 inches (15.2 centimeters) in diameter, but bin width readily changed to hold other sizes. Simple adjustments in bin-partition and web-belt positions needed to adapt system to different conduit cross sections.

Lewis, E. V.↗

Plasma and magnetospheric research

Several programs and variations have been developed to determine statistical means of different plasma parameters when binned in different variables. These parameters include temperature, densities and spacecraft potentials for any of the ion species, as well as ratios of these variables for any other ion species to the corresponding variable for H(+). The variables for binning include L, radial distance, and geomagnetic latitude; and separate statistics are automatically run for local morning and local evening data. These programs all run from output files from the plasma parameter thin sheath analysis program. A variant program also bins for magnetic activity, using either Kp or Dst, which requires an additional magnetic activity input file. These programs can be run either interactively or in batch mode, using file listings generated by a DIRECTORY command. In addition to printed output, these programs generate output files which can be used to plot the results. Programs to plot these averaged data are under development.

Comfort, R. H.↗

Description and availability of airborne Doppler radar data

An airborne, forward-looking, pulse, Doppler radar has been developed in conjunction with the joint FAA/NASA Wind Shear Program. This radar represents a first in an emerging technology. The radar was developed to assess the applicability of an airborne radar to detect low altitude hazardous wind shears for civil aviation applications. Such a radar must be capable of looking down into the ground clutter environment and extracting wind estimates from relatively low reflectivity weather targets. These weather targets often have reflectivities several orders of magnitude lower than the surrounding ground clutter. The NASA radar design incorporates numerous technological and engineering achievements in order to accomplish this task. The basic R/T unit evolved from a standard Collins 708 weather radar, which supports specific pulse widths of 1-7 microns and Pulse Repetition Frequencies (PRF) of less than 1-10 kHz. It was modified to allow for the output of the first IF signal, which fed a NASA developed receiver/detector subsystem. The NASA receiver incorporated a distributed, high-speed digital attenuator, producing a range bin to range bin automatic gain control system with 65 dB of dynamic range. Using group speed information supplied by the aircraft's navigation system, the radar signal is frequency demodulated back to base band (zero Doppler relative to stationary ground). The In-phase & Quadrature-phase (I/Q) components of the measured voltage signal are then digitized by a 12-bit A-D converter (producing an additional 36 dB of dynamic range). The raw I/Q signal for each range bin is then recorded (along with the current radar & aircraft state parameters) by a high-speed Kodak tape recorder.

Harrah, S. D.↗

Channel to energy calibration results for the BATSE large area detectors

Continuum 16 channel spectra obtained from in flight data are used to identify and correct for nonlinearities in the channel-to-energy conversion algorithm for the BATSE large area detectors. The Crab Nebula spectra obtained by the BATSE earth occultation technique are used to characterize any nonlinearities inherent in the low energy channel bin widths on a detector by detector basis. The bin widths are optimized to remove distortions from observed gamma ray spectra. The recalibrated bin edges are used in an analysis of Crab Pulsar data to verify the improvements in the calibration.

Pendleton, Geoffrey N.↗

Magnetopause shape as a bivariate function of interplanetary magnetic field B(sub z) and solar wind dynamic pressure

We present a new method for determining the shape of the magnetopause as a bivariate function of the hourly averaged solar wind dynamic pressure (p) and the north-south component of the interplanetary magnetic field (IMF) B(sub z). We represent the magnetopause (for X(sub GSE) greater than -40 R(sub E)) as an ellipsoid of revolution in solar-wind-aberrated coordinates and express the (p, B(sub z)) dependence of each of the three ellipsoid parameters as a second-order (6-term) bivariate expansion in Inp and B(sub z). We define 12 overlapping bins in a normalized dimensionless (p, B(sub z)) `control space' and fit an ellipsoid to those magnetopause crossings having (p, B(sub z)) values within each bin. We also calculate the bivariate (Inp, B(sub z)) moments to second order over each bin in control space. We can then calculate the six control-space expansion coefficients for each of the three ellipsoid parameters in configuration space. From these coefficients we can derive useful diagnosis of the magnetopause shape as joint functions of p and B(sub z): the aspect ratio of the ellipsoid's minor-to-major axes; the flank distance, radius of curvature, and flaring angle (at X(sub GSE) = 0); and the subsolar distance and radius of curvature. We confirm and quantify previous results that during periods of southward B(sub z) the subsolar magnetopause moves inward, while at X(sub GSE) = 0 the flank magnetopause moves outward and the flaring angle increases.

Roelof, Edmond C.↗

A Search for EUV Emission from the O4f Star Zeta Puppis

We obtained a 140 ks EUVE observation of the O4f star, zeta Puppis. Because of its low ISM column density and highly ionized stellar wind, a unique EUV window is accessible for viewing between 128 to 140 A, suggesting that this star may he the only O star observable with the EUVE. Although no SW spectrometer wavelength bin had a signal to noise greater than 3, a bin at 136 A had a signal to noise of 2.4. This bin is where models predict the brightest line due to OV emission should occur. We present several EUV line emission models. These models were constrained by fitting the ROSAT PSPC X-ray data and our EUVE data. If the OV emission is real, the best fits to the data suggest that there are discrepancies in our current understanding of EUV/X-ray production mechanisms. In particular, the emission measure of the EUV source is found to be much greater than the total wind emission measure, suggesting that the EUV shock must produce a very large density enhancement. In addition, the location of the EUV and X-ray shocks are found to be separated by approx. 0.3 stellar radii, but the EUV emission region is found to be approx. 400 times larger than the X-ray emission region. We also discuss the implications of a null detection and present relevant upper limits.

Waldron, Wayne L.↗

Willpower

I am struck by how often failure is blamed on a lack of discipline. You often hear losing coaches cite this as the reason for a big loss. I don't recall the last time I heard one say that his team lost a game because of his players' lack of skill. I think a breakdown in discipline is also one of the key reasons why program and project management teams fail to meet expectations. The first program I ever managed had a clear set of priorities. I understood the mandate, and so did everyone else on the team. We set an ambitious schedule and started to work fervently. Not too long into the program the customer wanted to know what performance he was going to get. I replied by categorizing the performance parameters into three bins: 1. Performance you will get. 2. Performance you may get. 3. Performance that there's no way you will get. Did that cause an uproar. The customer demanded everything in the second bin be moved to the first, and most everything in the third bin moved to the second. My immediate impulse was to agree, but I managed to overcome that. In my heart, I knew that we would never meet the already ambitious schedule if we had to deliver more performance. No was my answer. The program turned out to be a huge success, but the result would have been largely different had senior management or I failed to maintain discipline.

Little, Terry↗