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At least 235 records · Page 13

BioID/LID System Cartridge Testing Report

LLNL produced two lots of lyophilized LAMP assay containing cartridges, LNL022818 and LNL071818, for testing on Gen3 BioID systems. Thirty-nine cartridges were tested for lot LNL022818. All thirty-nine cartridges performed as expected regarding correct positive control (IPC) and negative control (NTC) reactions. Five different templates were tested on these 39 cartridges. The BioID system detected 79.1% true positives and 9.3% false positives along with 98.9% true negatives and 2.9% false negatives for cartridge lot LNL022818. Thirty cartridges were tested for lot LNL071818. It was noted early that due to a manufacturing issue with the cartridges related to incomplete sonic welding of the cartridge, a large fraction of lot LNL071818 cartridges leaked. Insufficient funds were available to replace this lot of cartridges. Twenty three of 30 cartridges performed as expected regarding correct positive control (IPC) and negative control (NTC) reactions. Four different templates were tested on these 30 cartridges. The BioID system detected 77% true positives and 23% false positives along with 96.9% true negatives and 3.1% false negatives for cartridge lot LNL071818. For all tested cartridges using DNA template, DNA was added at a final concentration of 25 pg/ul. All reactions contained positive control DNA at 2.5 pg/ul.

42 ENGINEERING↗

Acoustic Tomography System (CRADA Final Report)

NREL will install the CU/CIRES acoustic tomography array at the National Wind Technology Center (NWTC). The system consists of 9 freestanding towers of 9.1m height that are currently located at the NWTC. On these towers are installed a series of microphones and speakers. One center tower holds a sonic anemometer and temperature probe. The purpose of the acoustic tomography system is to measure complex flow fields surrounding wind turbine arrays that influence wind farm energy production and reliability. There is currently no funding to test and research the system (just install it), but NREL and CU researchers will write proposals that will fund the actual research with this unique measurement technology.

17 WIND ENERGY↗

Wind River Experimental Forest Subcanopy Tower Information Sheet

Wind River was one of three sites that collected 3d sonic anemometer data for an ICOS subcanopy observation study. The three sites were defined by the following features and terrain: a deciduous broadleaf forest in flat terrain (Lanžhot, Czech Republic), a coniferous forest in mountainous terrain (Renon, Italy), and a tall conifer forest in mountain-valley terrain (Wind River, USA). The Wind River subcanopy towers were deployed in a high LAI, old-growth evergreen conifer forest and collected approximately 11 months of data. The site is an ecologically rich temperate rainforest in the western Cascade Mountains, and the biological carbon sink and source strength has been measured since 1998 using eddy covariance on the top of a 74 m tall flux tower (currently called the Wind River NEON tower). Additionally, forest inventory records date back to the 1920s. In 2024, four subcanopy towers were installed near the Wind River NEON tower to measure wind flow in the understory canopy layer for better understanding canopy flow coupling and decoupling in the subcanopy and how this affects the interpretation of overstory fluxes. The subcanopy tower installation was done by Lawrence Livermore National Laboratory and Washington State University (WSU) with collaborations from the University of Utah and the National Ecological Observatory Network (NEON).

54 ENVIRONMENTAL SCIENCES↗

Prairie State Generating Company Static and Dynamic Modeling

A reservoir modeling study was conducted to assess the feasibility of storing 162.5 million tonnes (8.125 million tonnes annually) of industrially sourced carbon dioxide (CO 2 ) in the St. Peter – Everton and Knox storage complexes at the Prairie State Generating Company’s (PSGC) site near Marissa, Washington County, Illinois (PSGC site). Two separate models were constructed for the St. Peter – Everton and the Knox storage complexes. The St. Peter and Everton sandstones are the target storage units for the St. Peter – Everton storage complex. The Knox Group formations are the target storage units for the Knox storage complex. The Maquoketa Shale is the primary confining unit for both storage complexes. The static reservoir models used as input to the dynamic reservoir models of the storage complexes were developed from interpretations of logs (e.g., gamma ray, resistivity, porosity, photoelectric, and sonic), structure surfaces using data from 36 wells, thickness maps, well test data, seismic data, and permeability data in PetrelTM.

01 COAL, LIGNITE, AND PEAT↗