Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “rat”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

223 records · Page 13
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Dose Response, Dosimetric, and Metabolic Evaluations of Replacement PFAS Perfluoro-(2,5,8-trimethyl-3,6,9-trioxadodecanoic) Acid (HFPO-TeA)

Few studies are available on the environmental and toxicological effects of perfluoroalkyl ether carboxylic acids (PFECAs), such as GenX, which are replacing legacy PFAS in manufacturing processes. To collect initial data on the toxicity and toxicokinetics of a longer-chain PFECA, male and female Sprague Dawley rats were exposed to perfluoro-(2,5,8-trimethyl-3,6,9-trioxadodecanoic) acid (HFPO-TeA) by oral gavage for five days over multiple dose levels (0.3–335.2 mg/kg/day). Clinically, we observed mortality at doses >17 mg/kg/day and body weight changes at doses ≤ 17 mg/kg/day. For the 17 mg/kg/day dose level, T3 and T4 thyroid hormone concentrations were significantly decreased (p < 0.05) from controls and HFPO-TeA plasma concentrations were significantly different between sexes. Non-targeted analysis of plasma and in vitro hepatocyte assay extractions revealed the presence of another GenX oligomer, perfluoro-(2,5-dimethyl-3,6-dioxanonanoic) acid (HFPO-TA). In vitro to in vivo extrapolation (IVIVE) parameterized with in vitro toxicokinetic data predicted steady-state blood concentrations that were within seven-fold of those observed in the in vivo study, demonstrating reasonable predictivity. The evidence of thyroid hormone dysregulation, sex-based differences in clinical results and dosimetry, and IVIVE predictions presented here suggest that the replacement PFECA HFPO-TeA induces a complex and toxic exposure response in rodents.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory's High Flux Isotope Reactor

Neutrons have historically been used for a broad range of biological applications employing techniques such as small-angle neutron scattering, neutron spin echo, diffraction, and inelastic scattering. Unlike neutron scattering techniques that obtain information in reciprocal space, attenuation-based neutron imaging measures a signal in real space that is resolved on the order of tens of micrometers. The principle of neutron imaging follows the Beer-Lambert law and is based on the measurement of the bulk neutron attenuation through a sample. Greater attenuation is exhibited by some light elements (most notably, hydrogen), which are major components of biological samples. Contrast agents such as deuterium, gadolinium, or lithium compounds can be used to enhance contrast in a similar fashion as it is done in medical imaging, including techniques such as optical imaging, magnetic resonance imaging, X-ray, and positron emission tomography. For biological systems, neutron radiography and computed tomography have increasingly been used to investigate the complexity of the underground plant root network, its interaction with soils, and the dynamics of water flux in situ. Moreover, efforts to understand contrast details in animal samples, such as soft tissues and bones, have been explored. This manuscript focuses on the advances in neutron bioimaging such as sample preparation, instrumentation, data acquisition strategy, and data analysis using the High Flux Isotope Reactor CG-1D neutron imaging beamline. The aforementioned capabilities will be illustrated using a selection of examples in plant physiology (herbaceous plant/root/soil system) and biomedical applications (rat femur and mouse lung).

Bilheux, Hassina↗
DOE OSTI2021

KIC 10975348: A Double-mode or Triple-mode High-amplitude δ Scuti Star?

In this paper, we analyze the light variations of KIC 10975348 using photometric data delivered from the Kepler mission. This star is exceptionally faint (K {sub p} = 18.6 mag) compared to most well-studied δ Scuti stars. The Fourier analysis of the short-cadence data (i.e., Q14, Q15, and Q16, spanning 220 days) reveals that the variations are dominated by the strongest mode with a frequency of F0 = 10.231899 day{sup −1}, which is compatible with that obtained from RATS–Kepler. The other two independent modes with F1 (=13.4988 day{sup −1}) and F2 (=19.0002 day{sup −1}) are newly detected and have amplitudes two orders of magnitude smaller than F0. We note that, for the first time, this star is identified to be a high-amplitude δ Sct (HADS) star with an amplitude of about 0.7 mag, and the lower ratio of F0/F1 = 0.758 suggests that it might be a metal-rich variable star. The frequency F2 may be a third overtone mode, suggesting that this target might be a new radial triple-mode HADS star. We perform an O − C analysis using 1018 newly determined times of maximum light and derive an ephemeris formula of T {sub max} = 2456170.241912(0)+0.097734(1) × E. The O − C diagram shows that the pulsation period of KIC 10975348 seems to show no obvious change, which is in contrast to that of the majority of HADS stars. The possible cause of that may be due to the current short time span of the observations. To verify its possible period variations, regular observation from space with a longer time span in the future is needed.

47 OTHER INSTRUMENTATION↗
DOE OSTI2021

Interstellar Extinction, Polarization, and Grain Alignment in the Sh 2-185 (IC 59 and IC 63) Region

Optical and infrared continuum polarization from the interstellar medium is driven by radiative processes aligning the grains with the magnetic field. While a quantitative, predictive theory of radiative alignment torques (RATs) exists and has been extensively tested, several parameters of the theory remain to be fully constrained. In a recent paper, Medan & Andersson showed that the polarization efficiency (and therefore grain alignment efficiency) at different locations in the wall of the Local Bubble (LB) could be modeled as proportional to the integrated light intensity from the surrounding stars and OB associations. Here we probe that relationship at high radiation field intensities by studying the extinction and polarization in the two reflection nebulae IC 59 and IC 63 in the Sh 2-185 H ii region, illuminated by the B0 IV star γ Cassiopeia. We combine archival visual polarimetry with new seven-band photometry in the Vilnius system, to derive the polarization efficiency from the material. We find that the same linear relationship seen in the LB wall also applies to the Sh 2-185 region, strengthening the conclusion from the earlier study.

47 OTHER INSTRUMENTATION↗
DOE OSTI2020

The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction

Neurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain- and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active NMII light chains are colocalized with actin rings and organized in a circular periodic manner throughout the axon shaft. In contrast, NMII heavy chains are mostly positioned along the longitudinal axonal axis, being able to crosslink adjacent rings. NMII filaments can play contractile or scaffolding roles determined by their position relative to actin rings and activation state. We also show that MPS destabilization through NMII inactivation affects axonal electrophysiology, increasing action potential conduction velocity. In summary, our findings open new perspectives on axon diameter regulation, with important implications in neuronal biology.

Costa, Ana Rita↗
DOE OSTI2020

Sampling and resolution characteristics in reduced order models of shallow water equations: Intrusive vs nonintrusive

We investigate the sensitivity of reduced order models (ROMs) to training data spatial resolution as well as sampling rate. In particular, we consider proper orthogonal decomposition (POD), coupled with Galerkin projection (POD-GP), as an intrusive reduced order modeling technique. For non-intrusive ROMs, we consider two frameworks. The first is using dynamic mode decomposition (DMD), and the second is based on artificial neural networks (ANNs). For ANN, we utilized a residual deep neural network, and for DMD we have studied two versions of DMD approaches; one with hard thresholding and the other with sorted bases selection. Also, we highlight the differences between mean-subtracting the data (centering) and using the data without mean-subtraction. We tested these ROMs using a system of 2D shallow water equations for four different numerical experiments, adopting combinations of sampling rates and spatial resolutions. For these cases, we found that the DMD basis obtained with hard threshodling is sensitive to sampling rate. The sorted DMD algorithm helps to mitigate this problem and yields more stabilized and converging solution. Furthermore, we demonstrate that both DMD approaches without mean subtraction provide significantly more accurate results than DMD with mean-subtracting the data. On the other hand, POD is relatively insensitive to sampling rate and yields better representation of the flow field. Meanwhile, spatial resolution has little effect on both POD and DMD performances. Here, the numerical results reveal that an ANN on POD subspace (POD-ANN) performs remarkably better than POD-GP and DMD in capturing system dynamics, even with a small number of modes.

42 ENGINEERING↗