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The present invention relates a 3-dimensional cell to tissue and maintenance process, more particularly to methods of culturing cells in a culture environment, either in space or in a gravity field, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar spatial region.
Using the ROSAT and IRAS data achieves, we studied shadows in the diffuse 0.25 keV background toward infrared cirrus clouds to infer the distribution of the hot interstellar medium (ISM). Surprisingly, we found that anticorrelation between infrared emission from cirrus clouds and background x-ray intensity was present in all five galactic latitude (b approx. greater than 25 deg) fields that we selected. In two fieds, the anticorrelation is more than 30% deep, and shows particularly convincing evidence that they are due to x-ray absorption by the clouds. Assuming an average density for the local x-ray-emitting gas (T approximately 10(exp 6) K), we estimated the minimum distances to the clouds based on our best-fit x-ray intensities originating in front of the clouds. These distances are in the range of 60 to 160 pc. Therefore, we conclude that there are a considerable number of degree-size, x-ray-absorbing gas clouds at approximately 10(exp 2) pc and that large amounts of x-ray-emitting gas exist in regions beyonds these clouds.
An improved three-dimensional cell to tissue development process using a specific time varying electromagnetic force, pulsed, square wave, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar spatial region.
During the recent solar minimum, solar activity reached the lowest levels observed during the space age. This extremely low solar activity has accompanied a number of unexpected observations in the Earth's ionosphere and thermosphere when compared to previous solar minima. Among these are the fact that the ionosphere is significantly contracted beyond expectations based on empirical models. Climatological altitude profiles of ion density and composition measurements near the magnetic dip equator are constructed from the C/NOFS satellite to characterize the shape of the top side ionosphere during the recent solar minimum and into the new solar cycle. The variation of the profiles with respect to local time, season, and solar activity are compared to the IRI-2007 model. Building on initial results reported by Heelis et al. [2009], here we describe the extent of the contracted ionosphere, which is found to persist throughout 2009. The shape of the ionosphere during 2010 is found to be consistent with observations from previous solar minima.
During the recent solar minimum, solar activity reached the lowest levels observed during the space age. This extremely low solar activity has accompanied a number of unexpected observations in the Earth s ionosphere-thermosphere system when compared to previous solar minima. Among these are the fact that the ionosphere is significantly contracted beyond expectations based on empirical models. Altitude profiles of ion density and composition measurements near the magnetic dip equator are constructed from the Communication/Navigation Outage Forecast System (C/NOFS) satellite to characterize the shape of the topside ionosphere during the recent solar minimum and into the new solar cycle. The variation of the profiles with respect to local time, season, and solar activity are compared to the IRI-2007 model. Building on initial results reported by Heelis et al. (2009), here we describe the extent of the contracted ionosphere, which is found to persist throughout 2009. The shape of the ionosphere during 2010 is found to be consistent with observations from previous solar minima.
Simulated annealing is used to solve a minimum fuel trajectory problem in the space station environment. The environment is unique because the space station will define the first true multivehicle environment in space. The optimization yields surfaces which are potentially complex, with multiple local minima. Because of the likelihood of these local minima, descent techniques are unable to offer robust solutions. Other deterministic optimization techniques were explored without success. The simulated annealing optimization is capable of identifying a minimum-fuel, two-burn trajectory subject to four constraints. Furthermore, the computational efforts involved in the optimization are such that missions could be planned on board the space station. Potential applications could include the on-site planning of rendezvous with a target craft of the emergency rescue of an astronaut. Future research will include multiwaypoint maneuvers, using a knowledge base to guide the optimization.
The effect of thermal and caloric imperfections in cryogenic nitrogen on boundary layers was determined to indicate that in order to simulate nonadiabatic laminar or turbulent boundary layers in a cryogenic nitrogen wind tunnel, the flight enthalpy ratio, rather than the temperature ratio, should be reproduced. The absence of significant real gas effects on both viscous and inviscid flows makes it unlikely that there will be large real gas effects on the cryogenic tunnel simulation of shock boundary layer interactions or other complex flow conditions encountered in flight. Condensation effects were studied to determine the minimum usable temperature and indicated that under most circumstances free stream Mach number rather than maximum local Mach number determines the onset of condensation effects.
Virtual reality can enable a robot user to off line generate and test in a virtual environment a sequence of operations to be executed by the robot in an assembly cell. Virtual models of objects are to be correlated to the real entities they represent by means of a suitable transformation. A solution to the correlation problem, which is basically a problem of 3-dimensional adjusting, has been found exploiting the surface matching theory. An iterative algorithm has been developed, which matches the geometric surface representing the shape of the virtual model of an object, with a set of points measured on the surface in the real world. A peculiar feature of the algorithm is to work also if there is no one-to-one correspondence between the measured points and those representing the surface model. Furthermore the problem of avoiding convergence to local minima is solved, by defining a starting point of states ensuring convergence to the global minimum. The developed algorithm has been tested by simulation. Finally, this paper proposes a specific application, i.e., correlating a robot cell, equipped for biomedical use with its virtual representation.
One of the foremost requirements for accurate in-flight safety monitoring of autonomous unmanned aerial vehicles (UAVs) is tracking of their flight trajectory. Existing UAVs leverage autonomous flight functionalities based on trajectory generation algorithms developed in robotic applications such as polynomial or spline curves in order to facilitate kinematic smoothness, minimum vibrations and fuel efficiency. However in practice, the actual path may be subjected to unexpected local weather conditions, unexpected obstacles along the path or abrupt traffic changes in the low-altitude airspace resulting in large errors of the predicted time-of-arrival at way-points. In this study, an approach to quantify and propagate uncertainty in 4D trajectories is proposed. The paper presents a simple error interval propagation method based on the expected cruise speed of the UAV and its associated uncertainty. The uncertainty is then propagated in time to estimate reasonable confidence intervals on the times-of-arrival of the vehicle at each way-point as well as along the entire flight-path. The uncertainty propagation is demonstrated on a state-of-the-art trajectory generation algorithm based on non-uniform rational B-spline (NURBS) curves. Further, the effect of a stationary wind field is incorporated in the uncertainty propagation approach. The proposed method is implemented on synthetic and real data obtained from flight experiments with a small UAV.
Heat waves are extreme climate events that have the potential to cause immense stress on human health, agriculture and energy systems, so understanding the processes leading to their onset is crucial. There is no single accepted definition for heat waves, but they are generally described as a sustained amount of time where temperature exceeds a local threshold. Multiple different temperature variables are potentially relevant, as high values of daily maximum (T(max)) and minimum (T(min)) temperatures can both be detrimental to human health. Previous studies have concluded that the frequency of global heat waves has increased over recent decades, with greater increases in T(min)- than T(max)-heat waves in several regions. In this study, we focus explicitly on the different mechanisms associated with heatwaves manifest during daytime versus nighttime hours over the United States. Heat waves are examined using the National Aeronautics and Space Administration (NASA) Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2). A daytime (nighttime) heat wave is defined as average daytime (nighttime) temperature exceeding its calendar day 90th percentile for at least 3 days. Over 1980-2018, the number of heat wave days per summer has increased over much of the United States. Trends are stronger for nighttime versus daytime heat wave frequency over the Northeast, Midwest and Southwest United States. Local and remote processes linked with daytime and nighttime heat waves are identified through composite analysis of clouds, precipitation, soil moisture, and fluxes of heat and moisture. Finally, we characterize the large-scale atmospheric circulation associated with daytime and nighttime heat waves over different regions of the United States.
Voyager 2 magnetic field and plasma data are examined over time intervals of 1 to 12 hours in the heliospheric range of 1 to 10 AU to study the evolution of the anisotropy of solar wind fluctuations. Consistent with previous results, the directions of minimum variance vectors of magnetic fluctuations are found to be close to the mean magnetic field direction with an increasing component along the field at larger scales. At large radial distances there is more spread in the minimum variance directions than at smaller radial distances. The power in smaller-scale fluctuations in the magnetic field components perpendicular to the local mean field B(0) is in the ratio of about 5:1 near 1 AU at the scale of 1 hour but decreases to about 3:1 further out. No evidence for selective enhancement of out-of-the-ecliptic components of fluctuations is found. In contrast to results for field fluctuations, analysis of velocity fluctuations shows that the minimum variance direction systematically remains more radially oriented and becomes increasingly less oriented along B(0) with increasing heliocentric distance. The velocity fluctuations are generally more isotropic than the magnetic fluctuations. The observations cannot be explained by a superposed wave picture, and thus are consistent with the view that nonlinear turbulent evolution is responsible for the anisotropy in the fluctuations.
Computed energies and geometries are reported which, combined with previously published calculations, permit a global representation of the potential energy surface for the reaction H + O2 yields HO2(asterisk) yields OH + O. These new calculations characterize the potential energy surface (PES) for all H atom angles of approach to O2 and for the region of the inner repulsive wall. The region of the T-shaped H-O2 exchange saddle point is connected with the constrained energy minimum (CEM) path, and a new collinear H-O2 exchange saddle point is characterized which lies only 9 kcal/mol above the H + O2 asymptote. A vibrational analysis which utilizes local cubic and quartic polynomial representations of the PES along the CEM path has been carried out. Optimal geometries, energies, and harmonic frequencies are reported along with anharmonic analyses for the O2 and OH asymptotes and for the HO2 minimum region of the PES.
The values of the mistuning which yield the most stable eigenvectors are analytically determined, using the simplified equations of motion which were developed in Part I of this work. It is shown that random mistunings, if large enough, may lead to the maximal stability, whereas the alternate mistunings cannot. The problem of obtaining maximum stability for minimal mistuning is formulated, based on numerical optimization techniques. Several local minima are obtained using different starting mistuning vectors. The starting vectors which lead to the global minimum are identified. It is analytically shown that all minima appear in multiplicities which are equal to the number of compressor blades. The effect of mistuning on the flutter speed is studied using both an optimum mistuning vector and an alternate mistuning vector. Effects of mistunings in elastic axis locations are shown to have a negligible effect on the eigenvalues. Finally, it is shown that any general two-dimensional bending-torsion system can be reduced to an equivalent uncoupled torsional system.
The present analysis of electric field measurements from the Dynamics Explorer 2 satellite, which extends previous empirical models, emcompasses much data from polar crossings entering and exiting the high latitudes in all magnetic local time zones. The goal is to represent the typical distributions of convective electric fields with a minimum number of characteristic patterns. Significant large-scale revisions of the OGO 6 dawn-dusk measurement models are made. The deformations of the two-cell patterns lead to sunward convection in dayside polar regions, while maintaining the integrity of the nightside convection pattern.
The present treatment of dynamo-generated magnetic fields in the primordial solar nebula proceeds in view of the ability of the combined action of Keplerian rotation and helical convention to generate, via alpha-omega dynamo, large-scale magnetic fields in those parts of the nebula with sufficiently high, gas-and magnetic field coupling electrical conductivity. Nebular gas electrical conductivity and the radial distribution of the local dynamo number are calculated for both a viscous-accretion disk model and the quiescent-minimum mass nebula. It is found that magnetic fields can be easily generated and maintained by alpha-omega dynamos occupying the inner and outer parts of the nebula.
The Ozone Monitoring Instrument (OMI) on the Aura satellite obtains unique measurements for polar mesospheric cloud (PMC) analysis. Its wide cross-track viewing swath and high along-track spatial resolution makes it possible to directly evaluate PMC occurrence frequency and brightness variations between 6S" and 8S' latitude as a function of local time over a 12-14 h continuous period. OMI PMC local time variations are closely coupled to concurrent variations in measurement scattering angle, so that ice phase function effects must be considered when interpreting the observations. Two different phase functions corresponding to bright and faint clouds are examined in this analysis. OMI observations show maximum frequency and albedo values at 8-10 h local time in the Northern Hemisphere, with decreasing amplitude at higher latitudes. Southern Hemisphere values reach a minimum at 18-20 h LT. Larger variations are seen in Northern Hemisphere data. No statistically significant longitudinal dependence was seen.
The relationship between nuctilucent clouds (NLC), which are observed from the ground usually within 2 or 3 hrs of local midnight, and the polar mesospheric clouds (PMC), which are observed by satellites in full daylight, as well as the reason for the differences in their optical properties and their observed heights are investigated. Based on a suggestion that these differences can be attributed to a diurnal variation in the properties of a single type of cloud, two published models of the diurnal and semidiurnal variations of temperature, vertical wind speed, and eddy diffusion coefficient at high latitude to simulate the evolution of ice clouds over a 24-hr period. The results show that the minimum in temperature at about 2000 hours LT causes a sharp maximum in scattered brightness to occur about 1 hour before local midnight, with up to a factor of 7 variation in cloud brightness between noon and midnight. It is noted, however, that considerable uncertainties exist in these tidal models.
Determinations of boundary-layer transition on the NACA 0012 and 2301 airfoils were made in the 8-foot high-speed wind tunnel over a range of Reynolds Numbers from 1,600,000 to 16,800,000. The results are of particular significance as compared with flight tests and tests in wind tunnels of appreciable turbulence because of the extremely low turbulence in the high-speed tunnel. A comparison of the results obtained on NACA 0012 airfoils of 2-foot and 5-foot chord at the same Reynolds Number permitted an evaluation of the effect of compressibility on transition. The local skin friction along the surface of the NACA 0012 airfoil was measured at a Reynolds Number of 10,000,000. For all the lift coefficient at which tests were made, transition occurred in the region of estimated laminar separation at the low Reynolds Numbers and approach the point of minimum static pressure as a forward limit at the high Reynolds Numbers. The effect of compressibility on transition was slight. None of the usual parameters describing the local conditions in the boundary layer near the transition point served as an index for locating the transition point. As a consequence of the lower turbulence in the 8-foot high-speed tunnel, the transition points occurred consistently farther back along the chord than those measured in the NACA full-scale tunnel. An empirical relation for estimating the location of the transition point for conventional airfoils on the basis of static-pressure distribution and Reynolds Number is presented.