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At least 235 records · Page 13

All sources NASA NTRS DOE OSTI

DOE OSTI2023

Draft genome sequence of Yarrowia lipolytica NRRL Y-64008, an oleaginous yeast capable of growing on lignocellulosic hydrolysates

ABSTRACT Yarrowia lipolytica is an oleaginous yeast that produces high titers of fatty acid-derived biofuels and biochemicals. It can grow on hydrophobic carbon sources and lignocellulosic hydrolysates. The genome sequence of Y. lipolytica NRRL Y-64008 is reported to aid in its development as a biotechnological chassis for producing biofuels and bioproducts.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

Draft Genome Sequence of Cupriavidus necator DVZ60, an Isolate from Iodine-Impacted Hanford Groundwater

Cupriavidus necator DVZ60 was isolated from a sediment trap deployed in an 129I groundwater plume at the Hanford Site (Washington, United States). Genome sequencing of the strain led to the generation of 84 contigs and indicated that the genome is 7.552 Mb in size, with 66.2% GC content.

Hanford↗

DOE OSTI2021

Draft Genome Sequence of Stenotrophomonas maltophilia Strain PE591, a Polyethylene-Degrading Bacterium Isolated from Savanna Soil

We report the genome sequence of a polyethylene-degrading bacterial strain identified as Stenotrophomonas maltophilia strain PE591, which was isolated from plastic debris found in savanna soil. The genome was assembled in 16 scaffolds with a length of 4,751,236 bp, a GC content of 66.5%, and 4,432 predicted genes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

High-quality draft genome sequence of Thermobifida halotolerans DSM 44931

Here, we report the genome sequence of Thermobifida halotolerans DSM 44931, a bacterium that was originally isolated from a salt mine in the Yunnan Province of China. This genome was sequenced using Pacific Biosciences sequencing technology and was assembled into 2 contigs in 2 scaffolds. It has a total length of 5,506,851 bp and a GC content of 71.16%. Functional annotation of this genome provides further metabolic insight into this species.

actinomycete↗

DOE OSTI2023

Draft Genome Sequence of Enterobacter hormaechei DVZ29, an iodide-oxidizing bacterium isolated from the Hanford Site

Enterobacter hormaechei DVZ29 was isolated from a sediment trap incubated in an 129 I plume at the Hanford Site (Washington State, USA). Whole genome sequencing of the strain resulted in 32 contigs and revealed that the genome is 4.90 Mb, with a G+C content of 55.61%.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Draft genome sequences of two Pseudomonas strains isolated from 129 I plumes at the Hanford Site

Pseudomonas strains DVZ6 and DVZ24 were isolated from a sediment trap incubated in an 129 I plume at the Hanford Site (Washington State, USA). Whole-genome sequencing of the strains revealed that both genomes are 5.77 Mb in size, with a G + C content of 64.75%.

58 GEOSCIENCES↗

DOE OSTI2025

Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga

Haematococcus lacustris is a ubiquitous unicellular green alga with industrial bioproduct applications, namely, as feedstock for natural astaxanthin. We report the annotated 291.5 Mbp genome for H. lacustris Liv1 to support future algal research in the areas of carotenoid biosynthesis and crop protection.

bioproducts↗

DOE OSTI2020

Draft Genome Sequence of Pseudenhygromyxa sp. Strain WMMC2535, a Marine Ascidian-Associated Bacterium

Pseudenhygromyxa WMMC2535, a representative of the myxobacteria (family Nannocystaceae ), was isolated from a ragged sea hare in the Florida Keys, and its genome was sequenced using PacBio technology. The WMMC2535 genome sequence is the first of this genus and validates the notion that myxobacteria represent outstanding sources of structurally diverse natural products.

Chanana, Shaurya↗

DOE OSTI2020

Draft Genome Sequence of the Redox-Active Enteric Bacterium Citrobacter portucalensis Strain MBL

We grew a soil enrichment culture to identify organisms that anaerobically oxidize phenazine-1-carboxylic acid. A strain of Citrobacter portucalensis was isolated from this enrichment and sequenced by both Illumina and PacBio technologies. It has a genome with a length of 5.3 Mb, a G+C content of 51.8%, and at least one plasmid.

Tsypin, Lev M.↗

DOE OSTI2022

Friend or Foe: Draft Genome Sequence of Bradyrhizobium sp. Strain SRS-191

We report the genomic features of Bradyrhizobium sp. strain SRS-191, which was isolated from a former nuclear legacy site in Aiken, South Carolina, USA. With a genome size of 7,621,400 bp, the strain harbored genes not only for environmentally beneficial traits (e.g., heavy metal resistance, nitrogen fixation, and aromatic biodegradation) but also for antimicrobial resistance.

Chauhan, Ashvini↗

DOE OSTI2025

Draft genome sequence of Clostridium tetanomorphum DSM 4474T isolated from a septic wound

We report the genome sequence of Clostridium tetanomorphum DSM 4474T isolated from a septic wound. In addition to its potential as a human pathogen, this species may be useful in biofuel production due to its ability to synthesize butanol without the byproduct acetone. The genome is 4,374,054 bp.

Clostridium↗

DOE OSTI2019

Draft Genome Sequence of the Ectomycorrhizal Ascomycete Sphaerosporella brunnea

Sphaerosporella brunnea is a pioneer ectomycorrhizal fungus with facultative saprophytic capacities. Here, we sequenced the genome of S. brunnea strain Sb_GMNB300, which is estimated at 51.6 Mb in size with 872 assembled contigs accounting for 12,597 predicted coding genes. This genome will be useful for comparative studies of Pezizales ectomycorrhizal symbioses.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2018

High-Quality Draft Genome Sequences of Eight Bacteria Isolated from Fungus Gardens Grown by Trachymyrmex septentrionalis Ants

For their food source, Trachymyrmex septentrionalis ants raise symbiotic fungus gardens that contain bacteria whose functions are poorly understood. Here, we report the genome sequences of eight bacteria isolated from these fungus gardens to better describe the ecology of these strains and their potential to produce secondary metabolites in this niche.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2019

Improved Draft Genome Sequence of Microbacterium sp. Strain LKL04, a Bacterial Endophyte Associated with Switchgrass Plants

ABSTRACT We report here the genome assembly and analysis of Microbacterium strain sp. LKL04, a Gram-positive bacterial endophyte isolated from switchgrass plants ( Panicum virgatum ) grown on a reclaimed coal-mining site. The 2.9-Mbp genome of this bacterium was assembled into a single contig encoding 2,806 protein coding genes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

Draft genome of the switchgrass head smut pathogen Tilletia maclaganii

Tilletia maclaganii is a smut fungal pathogen that causes significant biomass reduction of switchgrass ( Panicum virgatum ) used for animal forage and biofuel production. Here we present the annotated genome of T. maclaganii , strain Tm001-NY21, estimated at 42.79 Mb in size, in 53 assembled contigs and encoding 10,235 predicted genes. This genome will be important for future comparative studies of Ustilaginales across its geographic and host range.

PacBio↗

DOE OSTI2019

Draft Genome Sequence of Mn(II)-Oxidizing Bacterium Oxalobacteraceae sp. Strain AB_14

Biological Mn(II) oxidation produces reactive manganese oxides that help to mitigate metal contamination in the environment. Here, we present the genome of Oxalobacteraceae sp. strain AB_14, a species of Mn(II)-oxidizing bacteria (MOB) that is notable for its ability to catalyze Mn oxidation at low pH (5.5).

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2018

Draft Genome Assemblies of Five Robust Yarrowia lipolytica Strains Exhibiting High Lipid Production, Pentose Sugar Utilization, and Sugar Alcohol Secretion from Undetoxified Lignocellulosic Biomass Hydrolysates

Screening the genetic diversity of 45 Yarrowia lipolytica strains identified five candidates with unique metabolic capability and robustness in undetoxified switchgrass hydrolysates, including superior lipid production and efficient pentose sugar utilization. Here, we report the genome sequences of these strains to study their robustness and potential to produce fuels and chemicals.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

One Complete and Seven Draft Genome Sequences of Subdivision 1 and 3 Acidobacteria Isolated from Soil

We report eight genomes from representatives of the phylum Acidobacteria subdivisions 1 and 3, isolated from soils. The genome sizes range from 4.9 to 6.7 Mb. Genomic analysis reveals putative genes for low- and high-affinity respiratory oxygen reductases, high-affinity hydrogenases, and the capacity to use a diverse collection of carbohydrates.

59 BASIC BIOLOGICAL SCIENCES↗