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At least 235 records · Page 13

Evolving Competitive Markets in SAPP: Leveraging Competitive Wholesale Electricity Markets to Drive Renewable Generation Capacity in the Southern African Power Pool (SAPP)

The SADC region has significant natural resource potential to increase renewable energy generation, improve electricity reliability, and support economic development. This research finds an apparent lack of confidence from electricity infrastructure investors in SAPP wholesale electricity markets, which increases risk perception and lowers the likelihood of capital deployment. With respect to free market fundamentals, competitive market obstacles and renewable energy development obstacles are characterized. Stakeholders identified the top obstacles to well-functioning competitive markets as insufficient transmission infrastructure for interconnection and regional movement of electricity, dominance of national single-buyer markets, and lack of or weak nation-state regulatory frameworks. Stakeholders prioritized the top three obstacles for renewable energy development as a lack of viable commercial arrangements for variable renewable energy (VRE) balancing, lack of functional and consistent nation-level regulations, and higher project costs related to reliance on imported equipment. With respect to potential solution options, stakeholders prioritized the development of new cost allocation and finance methods to facilitate new transmission expansion, training to educate new or potential new market entrants on SAPP processes, as well as modeling and analysis of regional SAPP participation benefits disaggregated to the nation-state level. From these perspectives, this research identified strategy options for consideration including transitioning SAPP to a regional transmission operator (RTO) for operation and planning of cross-border transmission facilities and market administration, shifting operations of SAPP member transmission systems to Independent System Operators (ISOs), establishing a regional regulatory authority and enhancing market data transparency. Implementing these reforms is expected to be challenging, but not insurmountable, given the domestic political, legal, and jurisdictional complexities of the SADC region.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

The Iowa Tribe of Kansas and Nebraska: Advancing Clean, Resilient, and Sovereign Energy (Summary Report of Communities LEAP Activities)

The Iowa Tribe of Kansas and Nebraska (ITKN) is a federally recognized Native American Tribe located along the Missouri River on the border of northeast Kansas and southeastern Nebraska. There are over 800 residents (Tribal citizens and non-Tribal) who live on the reservation, as well as more than 500 people who visit or work on the reservation on a daily basis. The ITKN faces many energy challenges, including rising service costs and dozens of power outages annually that impact resident well-being and business activities on Tribal lands. Power service issues are made more challenging by the remoteness of the reservation, which is 20 miles from the nearest town. Despite this, the ITKN has a long history of cultural and economic resilience: Local self-reliance, environmental stewardship, respecting the carrying capacity of the land, and strengthening the community are Tribal communities' traditional strengths. Long-term energy goals for the ITKN are centered around achieving energy sovereignty. Priority actions include: (1) Establishing a Tribal Utility Authority (TUA) to promote social welfare and community development.; (2) Deploying renewable community microgrids with ground-mount solar arrays and sustainable energy storage systems to advance energy sovereignty, resilience, and reliability. To advance these goals, the ITKN partnered with the U.S. Department of Energy's (DOE's) Communities LEAP (Local Energy Action Program) pilot. From August 2022 to March 2024, the ITKN community coalition collaborated with technical assistance providers at DOE's National Renewable Energy Laboratory (NREL) and Sandia National Laboratories to evaluate TUA planning needs and microgrid deployment scenarios. This final report details the Communities LEAP technical assistance process, models and analysis performed, and results.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Idaho National Laboratory Integrated Multisite SSHAC Level 3: Probabilistic Volcanic Hazards Assessment

The Idaho National Laboratory (INL) resides on the eastern Snake River Plain (ESRP), part of the Snake River Plain (SRP) with a complex origin and geologic history of volcanism. Much of the Quaternary (last 2.58 million years) volcanism within 400 km of INL is genetically associated with a major thermal anomaly referred to as the Yellowstone hotspot, which is currently located more than 180 km northeast of INL. Near INL, local volcanic sources include silicic domes near its southern border, and numerous dike-fed basaltic vents of the ESRP, some of which are near or within the INL boundaries. Quantitative probabilistic assessments of screened-in volcanic hazardous phenomena for nine different facility complexes at the INL are presented for a Senior Seismic Hazard Analysis Committee (SSHAC) Level 3 (SL3) study. The comprehensive, integrated multisite SSHAC study consists of a single regional Probabilistic Volcanic Hazards Assessment (PVHA) that pertains to all nine INL facility complexes, with site-specific information developed at each respective area of interest (AOI), referred to as the "INL facility AOI" (Figure ES-1). Hazard products are generated for specified INL facility AOIs for use by multiple stakeholders from different agencies in risk-informed decision-making regarding site selection, operations, and design of nuclear facilities at INL, consistent with U.S. Department of Energy (DOE) and U.S. Nuclear Regulatory Commission (NRC) regulatory guidance. The study also serves as the basis for future periodic safety assessments required for existing DOE facilities, such as 10-year evaluations of natural-phenomena hazards. Elements of the INL PVHA including initial characterization, screening, quantitative assessments of eruption and hazard potential, and consideration of facility needs are conducted using the three phases of the SSHAC process: evaluation, integration, and documentation. As per regulatory guidance, the SSHAC framework provided the necessary processes and procedures for the PVHA Technical Integration (TI) team, at three workshops, five formal working meetings and many TI team remote meetings, to conduct initial characterization, screen the volcanic hazards, create PVHA model inputs, exercise those models in the PVHA, consider facility-specific volcanic hazard needs, and perform final hazard calculations. The Participatory Peer Review Panel (PPRP) provided independent oversight and performed process and technical reviews of the PVHA throughout its duration. The study included an extensive New Data Collection and Analyses (NDCA) program developed by the PVHA TI team to reduce uncertainties in hazard-significant elements in the PVHA model. NDCA activities generated 20 reports providing important contributory datasets and results to the project database for characterizing the ESRP. For example, a report compiling the dimensions of ESRP shield volcanoes and lava fields was used to construct volcanic footprints (areas of impact), was compared with data from INL subsurface cores, and was used to validate the results of lava-flow inundation modeling on the contemporary terrain. Another example is the acquisition of aeromagnetic data over INL and its surrounding area, with maps of buried magmatic features (e.g., subsurface volcanoes and swarms of feeder dikes) that informed the PVHA conceptual model of volcanism. The SSHAC evaluation process was used to conduct all elements of the PVHA including initial characterization and screening. Existing data and NDCA activities provided the foundation to develop the tectonomagmatic conceptual model of volcanism for the region of geographical interest in the SRP and Yellowstone hotspot volcanic system, and for considering volcanoes in the western US. Considering Quaternary volcanic sources active during this period, the screening approach identified and evaluated magma compositions, types of eruptive and intrusive phenomena, types of hazardous phenomena, and proximity of sources to INL. The PVHA TI team evaluated 18 types of magmatic sources in terms of 20 potentially hazardous phenomena, resulting in 360 screening decisions. The screening process resulted in 140 screened-in hazardous volcanic phenomena for Quaternary volcanic sources 1) proximal to INL facility complexes in the ESRP (64 basaltic and 54 silicic), 2) regional sources associated with the Yellowstone caldera system and Blackfoot Reservoir volcanic field (7), and 3) more distal sources from thirteen Cascade volcanoes and two volcanoes at Long Valley caldera (CA).

58 GEOSCIENCES↗

Tactical Analysis for Calculating Contextual Risk at Boundaries: Summary of Laboratory Directed Research & Development Effort

The Tactical Analysis for Calculating Contextual Risk at Boundaries (TACCRAB) tool is an innovative digital twin (DT) platform and automated risk algorithm designed to transform operational decision-making in structured screening environments, with an initial focus on Southern Border Land Ports of Entry (POEs). The invention provides integration points for advanced artificial intelligence, predictive modeling, and real-time data analysis to produce a comprehensive risk management tool that enables proactive, data-informed security strategies. The core inventive features of TACCRAB center on its unique risk algorithm, which dynamically calculates contextual risk by synthesizing historical data, near real-time streaming data from the checkpoints themselves, and AI-generated predictions. Unlike traditional risk assessment methods, TACCRAB utilizes a DT to provide comprehensive operational insights, allowing stakeholders to visualize, simulate, and optimize checkpoint configurations with unprecedented speed and contextual awareness. TACCRAB's key innovation lies in its ability to combine multiple complex inputs - including technology detection probabilities, resource availability, screening pathway characteristics, and threat actor behavioral patterns - into a unified risk calculation and update these inputs based on changing operational and environmental conditions. By leveraging a DT that continuously updates and learns from linked data, TACCRAB can suggest adaptive mitigation strategies that minimize risk while maintaining operational efficiency. Particularly novel is the platform's approach to decision support, which goes beyond static risk assessment. The DT provides dynamic metrics such as wait times, resource allocation effectiveness, and potential emerging threat scenarios, enabling users to view sophisticated, relevant what-if simulations and optimize checkpoint operations in near real-time. The system's architecture allows for generalized application across different screening environments, such as secure facilities, ports of entry, and soft targets, making it a versatile tool for security and operational management. The invention distinguishes itself through its comprehensive integration of predictive modeling, AI-driven pattern discovery, and user-friendly interface design. By combining these elements, TACCRAB transforms complex risk data into actionable insights, supporting decision-makers at various organizational levels - from booth agents making split-second screening decisions to checkpoint managers optimizing the day's resource allocation to strategic planners managing long-term investments.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Evaluating Movement Patterns of the Rattlesnake Hills Elk Herd on Hanford for Calendar Years 2019-2024

Biologists first documented elk on the Hanford Site in 1972; since then, the elk herd known as the Rattlesnake Hills Elk Herd (RHEH) has grown substantially (PNNL-13331, Population Characteristics and Seasonal Movement Patterns of the Rattlesnake Hills Elk Herd: Status Report 2000). Through the 1990s, the core range of the RHEH was focused on the portion of the Hanford Reach National Monument known as the Fitzner-Eberhardt Arid Lands Ecology (ALE) Reserve. More recently, larger numbers of elk have been occupying the U.S. Department of Energy (DOE), Hanford Field Office (HFO), formerly the DOE, Richland Operations Office managed portion- of the Hanford Site, known as central Hanford (Figure 1-1). This began with bachelor groups of bulls occupying the site intermittently, and the herd has grown to resident herds, including bulls, cows, and calves. The Washington Department of Fish and Wildlife (WDFW) established a target herd size for the RHEH of less than 350 animals to minimize damages on adjacent private agricultural lands (Washington State Elk Herd Plan–Yakima Elk Herd [WDFW 2002]). Attempts to control the RHEH population through hunting on private lands and a relocation effort during 2000 have failed to limit growth of the population toward the WDFW target, and the herd exceeds 1,600 according to recent counts (PNNL-13331; DOE/RL- 2023-20, Hanford Annual Site Environmental Report for Calendar Year 2022). Although elk are present on central Hanford year-round, they continue to move between areas offsite, the ALE Reserve, and central Hanford throughout the year, and it is these movements that result in many of the elk-vehicle collisions (EVC) that occur along Hanford Site roads and on the Washington State highways bordering the site. In addition, changes in management strategies on the ALE Reserve, including recent and potential future tribal elk hunts, may alter herd behavior and result in additional animals transiting onto and off central Hanford.

54 ENVIRONMENTAL SCIENCES↗

Thriving in the Carbon-Aware Market: How to Account for Emissions in the Era of Carbon-Centered Trade Policies

Emerging global policies, such as the European Union's enacted Carbon Border Adjustment Mechanism and similar policies under development in Canada, Australia and the United Kingdom, will place a premium on goods traded into their territories with higher embedded emissions than those produced domestically. U.S. manufacturers could stand to benefit from such policies; given the investments U.S. industry has made to reduce the energy and emission intensities of its operations. For example, the overall GHG intensity of U.S. steel production in 2019 was ~0.96 t CO2/t steel, less than half that of China (~1.97 t CO2/t steel), and bested only by Italy. To realize these benefits, transparent, accurate, interoperable and accepted embedded emissions accounting and calculation methods are required. Achieving this requires overcoming challenges related to data availability, boundary definitions, and product definitions among others, both at individual facilities as well as through value chains. We will present technical findings on methodology considerations and data-availability constraints for determining the emissions of traded goods, using steel as a pilot and leveraging publicly available data. Issues such as determining the appropriate scope for emissions accounting, implications of the specificity of product chosen, emissions allocation in multi-product facilities, and enumeration of emissions for products manufactured across multiple facilities will be discussed. By sharing the results of our efforts, we aim to inform the development and execution of embedded emissions accounting methods from a technical perspective such that U.S. manufacturers can thrive in emerging global markets.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Outcomes of PAX sapiens-Supported Global Wildlife Data Sharing Conferences for Enhanced One Health Security (GWDSC)

Across two consecutive Global Wildlife Data Sharing Conferences supported by PAX sapiens—Year 1 (May 2024) at Pacific Northwest National Laboratory and Year 2 (2025) in Ciudad Real, Spain—the initiative converted wildlife data sharing from aspiration into operational reality, producing measurable impacts in platform development, data mobilization, standards harmonization, and international partnership formation. The conferences addressed a critical gap in global health security: while 75% of emerging infectious diseases affect both humans and animals and over 60% originate in wildlife, wildlife health surveillance has historically lagged behind human and agricultural sectors due to fragmented databases, inconsistent terminology, uneven capacity, and limited cross-border coordination. By convening practitioners, government agencies, international organizations, academic institutions, and NGOs, the GWDSC catalyzed trust-based relationships and practical workflows that enable earlier detection, better risk assessment, and more effective prevention of threats at the wildlife–domestic animal–human–environment interface.

54 ENVIRONMENTAL SCIENCES↗

PSH Assessment and Site Identification [Slides]

NLR's Pumped Storage Hydropower (PSH) geospatial and cost model algorithms are applied to the Chemehuevi Reservation to assess the potential for PSH within the reservation. The algorithm identifies both "open-loop" PSH opportunities formed by constructing a new reservoir within the reservation paired with bordering Lake Havasu and "closed-loop" systems formed by two new reservoirs within the reservation. Options range from 28 to 538 MW of electrical generation power at maximum generation and 10 hours of storage. CAPEX is estimates as 4422 2022 $\$$/kW of generating power, which is meaningfully higher than the lowest cost systems identified in NLR's national scale assessments. Further economic analysis is needed to fully evaluate whether this would be an attractive option to meet the Chemehuevi Reservation's goals.

25 ENERGY STORAGE↗

Household Energy Use in Pennsylvania's Delaware Valley: An Energy-Focused Housing Stock Analysis

This report covers the housing stock analysis of the Energy to Communities Delaware Valley project. The analysis covers the housing stock of Bucks, Chester, Delaware, and Montgomery counties that border Philadelphia County in southeast Pennsylvania. It focuses primarily on single-family houses including their existing characteristics and energy consumption and the potential impact of various retrofit measure packages applied to them. This analysis was a collaborative effort among the project team, including national lab researchers and local organizations. It derives primarily from results of modeling tools from the national labs, especially NLR's ResStock, supplemented by key information from the study area.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

SPARTAN and IMPROVE Comparison Experiment (SPICE) Interim Campaign Report

SPICE (the SPARTAN and IMPROVE Comparison Experiment) aims to obtain and quantify comparisons between aerosol PM 2.5 mass concentration measurements from the University of Oklahoma (OU) Surface Particulate Matter Network (SPARTAN) station and the U.S. Environmental Protection Agency (EPA) Interagency Monitoring of Protected Visual Environments (IMPROVE) station hosted by the U.S. Department of Energy Atmospheric Radiation Measurement (ARM) User Facility at ARM’s Southern Great Plains (SGP) observatory in Oklahoma. Aerosols—both natural and anthropogenic—affect human populations in multiple ways. Much of ARM’s research focuses on how aerosols influence weather and climate through their optical and radiative effects, as well as their impacts on clouds and precipitation. However, aerosols also pose direct risks to humans and other organisms through inhalation, with the severity of health impacts depending on particle size, chemical composition, and duration of exposure. The IMPROVE network was established by the U.S. EPA to monitor air quality, including visible clarity as well as total and chemically speciated aerosol mass concentrations. The ARM SGP site hosts the IMPROVE SOGP station. Separately, the SPARTAN network operates a globally distributed set of stations similar to IMPROVE but with an emphasis on remote deployment and semi-autonomous operation for use beyond the borders of the United States (IMPROVE only operates within the U.S.). The University of Oklahoma operates a SPARTAN station. To establish confidence in the OU SPARTAN instrumentation and measurement protocol relative to the EPA-certified IMPROVE station, the OU SPARTAN station is currently deployed at SGP in close proximity to the IMPROVE SOGP station. The SPICE campaign was envisioned as a contiguous calendar-year effort for 2025 to capture seasonal variation in mass loading as well as composition. However, independent of the SPICE campaign, the SPARTAN network adopted a new filter construction part-way through the year, interrupting our contiguous data set. Thus, to obtain a contiguous data set with a uniform consistent configuration, SPICE desires an extension through 2026.

54 ENVIRONMENTAL SCIENCES↗

Analysis of Slow Spill Data for Mu2e

The Mu2e experiment requires a constant, relatively low intensity muon beam to produce data with high clarity, which can be achieved using slow extraction. Slow spills/extractions in the Delivery Ring involve contracting and expanding the stable region, which is bordered by the separatrix, of the beam pipe. While this does lower the beam intensity, it is very inconsistent. To help mitigate future inconsistencies, data from many trial spills (some including various magnet impulses to influence the beam intensity) was examined. This involved cutting low quality spills that have abnormal peak and integrated intensities, as well as spills with unusually low magnet ramping. Then, the remaining spills in the datasets were analyzed for trends within spills and across many spills. The findings from this analysis were then given to the FAN-C team to help them develop their simulations, as well as provide training data for their machine learning models that will use beam and impulse data to apply corrective impulses during future slow extractions.

Osborn, Thomas [Purdue U., West Lafayette]↗

Leaf-area index of Oak-Hickory Forest at Missouri Ozark (MOFLUX) site: 2007–2022

This data set contains measurements of leaf-area index (LAI) at the Missouri Ozark (MOFLUX) site during growing seasons from 2007-2022. The MOFLUX site is located in the University of Missouri Baskett Forest, a second growth oak-hickory forest situated in the Ozark Border Region of central Missouri, USA. MOFLUX is part of the AmeriFlux network (site ID: US-MOz) and an eddy covariance tower marks the middle of the site (Figure 1). During 2003, 24 circular vegetation plots (each 0.08 hectares) were established within a ~250 meter (m) radius around the tower. The plots were situated 50 m apart along 5 linear transects radiating out from the flux tower base in southeast, south, southwest, west, and northwest directions. There were 5 plots per transect except for the northwest one, which had only 4 due to the presence of a small pond at the terminus. At weekly intervals during the growing season, leaf-area index measurements were taken within each vegetation plot on the 5 transects running from the central flux tower. A plant canopy analyzer (model LAI-2000 Li-Cor Inc., Lincoln NE) was used to make the LAI measurements. We collected samples to enable measurement of the mean LAI of each transect. LAI observations are useful for understanding vegetation phenology, and to aid in the interpretation of ecosystem gas exchange. This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

ESS-DIVE CSV File Formatting Guidelines Reporting ↗

Methods of Securing Chemical and Pharmaceutical Knowledge and Recommendations for International Institutions to Enhance Research Integrity

Here, this paper examines strategies for securing chemical and pharmaceutical expertise in a globalized research environment, focusing on safeguarding intellectual property and preventing the misuse of sensitive and potentially dual-use information. The product of collective efforts between Pacific Northwest National Laboratory, Carol Davila University of Medicine and Pharmacy, and New Bulgarian University, highlights the challenges and opportunities posed by cross-border research collaborations, particularly in the context of differing regulatory frameworks and research cultures. It explores current mechanisms to prevent data loss and unauthorized access to sensitive information while assessing the effectiveness of existing security measures, frameworks, and international export control regimes. The approach examines the differing methodologies for promoting transparency, trust-building, and mutual accountability in joint research projects to cultivate secure data-sharing practices and intellectual property. It provides recommendations for international institutions to implement security guidelines in framing research priorities, encourages continual training and education programs, and the integration of processes for monitoring research compliance. This partnership aims to advance scientific innovation while maintaining global stability, ensuring compliance with international norms, and safeguarding valuable intellectual property as measures in chemical and pharmaceutical research security practices continue to expand due to international collaboration and knowledge exchange.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Intrinsic value of the international benchmark projects, ICSBEP and IRPhEP, for advanced reactor development

The International Criticality Safety Benchmark Evaluation Project, a sanctioned program under the auspices of the Nuclear Energy Agency of the Organisation for Economic Co-operation and Development, has been a highly successful and productive collaboration, now encompassing over 5,000 evaluated experimental benchmarks trusted and relied upon throughout the international nuclear communities. The success of this project led to the development of the International Reactor Physics Experiment Evaluation Project, which is dedicated to the evaluation of benchmark experiment data to sustain current and future reactor physics validation needs. These exemplary programs, and their widely utilized handbooks, serve as gold standards to which other databases strive to emulate. The purpose of the two projects is to preserve modern and legacy experimental data and evaluate it in a standardized handbook format to provide quality benchmarks to support modern and future criticality safety and reactor physics validation. These two projects have often served as the mechanism through which historic and modern neutronics experiments are evaluated and shared across international borders, to best provide unique, high-quality peer-reviewed, and often otherwise unavailable, benchmark data. The contents of these handbooks are utilized not only in validating criticality safety, reactor physics, and advanced reactor calculations, but are used to validate neutronics calculations and nuclear data for most other nuclear applications. This paper discusses both international programs and available content to enable advanced reactor design validation.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

The effect of monitoring complexity on stakeholder acceptance of CO2 geological storage projects in the US gulf coast region

Environmental monitoring at geologic CO 2 storage sites is required by regulations for the purposes of environmental protection and emissions accounting in the case of leakage to surface. However, another very important goal of environmental monitoring is to assure stakeholders that the project is monitored for safety and effectiveness. With current efforts to optimize monitoring for cost-effectiveness, the question remains: will optimization of monitoring approaches degrade stakeholder assurance, or do heavily-instrumented sites communicate higher risk to a stakeholder? We report the results of a stakeholder survey in Gulf Coast states of the US where carbon capture and storage (CCS) is developing quickly. We rely on a 2 by 2 factorial experiment in which we manipulate message complexity (complex v. simple) and social norm (support from scientists v. support from community members). Subjects were randomly assigned to one of four conditions: 1) complex message with scientist support; 2) complex message with community member support; 3) simple message with scientist support; or 4) simple message with community member support. In addition to the experimental stimuli, subjects were also asked about their need for cognition, attitudes toward science and scientists, attitudes about climate change and support for carbon capture and storage (CCS). Our sample is drawn from residents in states bordering the western Gulf of Mexico (Texas, Louisiana, Florida) where CO 2 geologic storage is being planned both onshore and offshore. The results offer important implications for public outreach efforts to key stakeholders.

54 ENVIRONMENTAL SCIENCES↗

Resistance, Resilience, and Recovery of Dryland Soil Bacterial Communities Across Multiple Disturbances

Dryland ecosystems are sensitive to perturbations and generally slow to recover post disturbance. The microorganisms residing in dryland soils are especially important as they contribute to soil structure and nutrient cycling. Disturbance can have particularly strong effects on dryland soil structure and function, yet the natural resistance and recovery of the microbial components of dryland soils has not been well documented. In this study, the recovery of surface soil bacterial communities from multiple physical and environmental disturbances is assessed. Samples were collected from three field sites in the vicinity of Moab, UT, United States, 6 to 7 years after physical and climate disturbance manipulations had been terminated, allowing for the assessment of community recovery. Additionally, samples were collected in a transect that included three habitat patches: the canopy zone soils under the dominant shrubs, the interspace soils that are colonized by biological soil crusts, and edge soils at the plot borders. Field site and habitat patch were significant factors structuring the bacterial communities, illustrating that sites and habitats harbored unique soil microbiomes. Across the different sites and disturbance treatments, there was evidence of significant bacterial community recovery, as bacterial biomass and diversity were not significantly different than control plots. There was, however, a small number of 16S rRNA gene amplicon sequence variants that distinguished particular treatments, suggesting that legacy effects of the disturbances still remained. Taken together, these data suggest that dryland bacterial communities may possess a previously unappreciated potential to recover within years of the original disturbance.

59 BASIC BIOLOGICAL SCIENCES↗

Unique Structural Features Relate to Evolutionary Adaptation of Cytochrome P450 in the Abyssal Zone

Cytochromes P450 (CYPs) form one of the largest enzyme superfamilies, with similar structural folds yet biological functions varying from synthesis of physiologically essential compounds to metabolism of myriad xenobiotics. Sterol 14α-demethylases (CYP51s) represent a very special P450 family, regarded as a possible evolutionary progenitor for all currently existing P450s. In metazoans CYP51 is critical for the biosynthesis of sterols including cholesterol. Here we determined the crystal structures of ligand-free CYP51s from the abyssal fish Coryphaenoides armatus and human-. Comparative sequence–structure–function analysis revealed specific structural elements that imply elevated conformational flexibility, uncovering a molecular basis for faster catalytic rates, lower substrate selectivity, and intrinsic resistance to inhibition. In addition, the C. armatus structure displayed a large-scale repositioning of structural segments that, in vivo, are immersed in the endoplasmic reticulum membrane and border the substrate entrance (the FG arm, >20 Å, and the β4 hairpin, >15 Å). The structural distinction of C. armatus CYP51, which is the first structurally characterized deep sea P450, suggests stronger involvement of the membrane environment in regulation of the enzyme function. We interpret this as a co-adaptation of the membrane protein structure with membrane lipid composition during evolutionary incursion to life in the deep sea.

Biochemistry & Molecular Biology↗

The Evolution of Galaxies and Clusters at High Spatial Resolution with Advanced X-ray Imaging Satellite (AXIS)

Stellar and black hole feedback heat and disperse surrounding cold gas clouds, launching gas flows off circumnuclear and galactic disks, producing a dynamic interstellar medium. On large scales bordering the cosmic web, feedback drives enriched gas out of galaxies and groups, seeding the intergalactic medium with heavy elements. In this way, feedback shapes galaxy evolution by shutting down star formation and ultimately curtailing the growth of structure after the peak at redshift 2–3. To understand the complex interplay between gravity and feedback, we must resolve both the key physics within galaxies and map the impact of these processes over large scales, out into the cosmic web. The Advanced X-ray Imaging Satellite (AXIS) is a proposed X-ray probe mission for the 2030s with arcsecond spatial resolution, large effective area, and low background. AXIS will untangle the interactions of winds, radiation, jets, and supernovae with the surrounding interstellar medium across the wide range of mass scales and large volumes driving galaxy evolution and trace the establishment of feedback back to the main event at cosmic noon. This white paper is part of a series commissioned for the AXIS Probe mission concept; additional AXIS white papers can be found at the AXIS website.

47 OTHER INSTRUMENTATION↗