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At least 235 records · Page 13

The Importance of Protein Phosphorylation for Signaling and Metabolism in Response to Diel Light Cycling and Nutrient Availability in a Marine Diatom

Diatoms are major contributors to global primary production and their populations in the modern oceans are affected by availability of iron, nitrogen, phosphate, silica, and other trace metals, vitamins, and infochemicals. However, little is known about the role of phosphorylation in diatoms and its role in regulation and signaling. We report a total of 2759 phosphorylation sites on 1502 proteins detected in Phaeodactylum tricornutum. Conditionally phosphorylated peptides were detected at low iron (n = 108), during the diel cycle (n = 149), and due to nitrogen availability (n = 137). Through a multi-omic comparison of transcript, protein, phosphorylation, and protein homology, we identify numerous proteins and key cellular processes that are likely under control of phospho-regulation. We show that phosphorylation regulates: (1) carbon retrenchment and reallocation during growth under low iron, (2) carbon flux towards lipid biosynthesis after the lights turn on, (3) coordination of transcription and translation over the diel cycle and (4) in response to nitrogen depletion. We also uncover phosphorylation sites for proteins that play major roles in diatom Fe sensing and utilization, including flavodoxin and phytotransferrin (ISIP2A), as well as identify phospho-regulated stress proteins and kinases. These findings provide much needed insight into the roles of protein phosphorylation in diel cycling and nutrient sensing in diatoms.

59 BASIC BIOLOGICAL SCIENCES↗

Precision and Limits of Detection for Selected Commercially Available, Low-Cost Carbon Dioxide and Methane Gas Sensors

The performance of a sensor platform for environmental or industrial monitoring is sensitive to the cost and performance of the individual sensor elements. Thus, the detection limits, accuracy, and precision of commercially available, low-cost carbon dioxide and methane gas concentration sensors were evaluated by precise measurements at known gas concentrations. Sensors were selected based on market availability, cost, power consumption, detection range, and accuracy. A specially constructed gas mixing chamber, coupled to a precision bench-top analyzer, was used to characterize each sensor during a controlled exposure to known gas concentrations. For environmental monitoring, the selected carbon dioxide sensors were characterized around 400 ppm. For methane, the sensor response was first monitored at 0 ppm, close to the typical environmental background. The selected sensors were then evaluated at gas concentrations of several thousand ppm. The determined detection limits accuracy, and precision provides a set of matrices that can be used to evaluate and select sensors for integration into a sensor platform for specific applications.

Honeycutt, Wesley T. (ORCID:0000000236814810)↗

Assessment of Potential Pennycress Availability and Suitable Sites for Sustainable Aviation Fuel Refineries in Ohio

Pennycress grain has a relatively high oil content (25–36%) and it is considered a desirable feedstock to produce sustainable aviation fuel (SAF). Pennycress crop can be integrated into the corn–soybean rotation as a winter cover crop in the midwestern U.S. to provide both ecosystem services and economic benefits for the farmers, while serving as a promising feedstock for SAF production. For pennycress-based SAF biorefineries to be established at the commercial scale, a sustainable design of the supply system is required to provide reliable information on feedstock availability and optimal facility locations. The objectives of this research were to assess the pennycress production potential in Ohio, and to identify the best locations to establish the SAF biorefineries. To estimate the pennycress production potential in Ohio, a geographic information system (GIS)-based model was developed using the spatially explicit six-year historical data on areas that were planted in the corn–soybean rotation for the period of 2013 through 2018, pennycress yield estimates from field-based experiments reported in the literature, and the soil productivity index for the region of study. Optimal SAF biorefinery locations were identified using a GIS-based location-allocation model. Annual land potentially available for pennycress production in Ohio was estimated to be ~0.6 million ha, which could produce ~1.1 million metric tons of pennycress grain as feedstock to produce ~210 million liters of SAF, depending on the pennycress yield level, oil content, and conversion efficiencies. In addition, the optimum locations for 12 biorefineries, each at an annual capacity of 18.9 million liters of SAF, were identified, and the average transportation distance was estimated to be 35 and 58 km for maximizing attendance and coverage conditions, respectively. The outcomes of this research would help minimize the risks associated with feedstock supply and cost variabilities for pennycress-based SAF production in the region.

Mousavi-Avval, Seyed Hashem↗

Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples

African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus.

59 BASIC BIOLOGICAL SCIENCES↗

Availability Estimates of Low-Cost, Dispatch-Constrained Electricity (LDE)

This dataset accompanies the report "Potential Availability of and Supply Curves for Low-Cost, Dispatch-Constrained Electricity", which provides initial estimates of the quantity and availability of the potential low-cost, dispatch-constrained electricity (LDE) resource in the United States under scenarios with high variable renewable energy (VRE) penetrations. The analysis also estimates supply curves that can be used in subsequent analysis of the opportunity to use the LDE. The estimated LDE resource could be used for various applications that value low-cost electricity and can operate at reduced capacity factors, including electrolytic hydrogen production and carbon capture. The analysis accompanying this dataset was performed in 2018-2019 to support an analysis of "The Technical and Economic Potential of the H2@Scale Concept within the United States" ( https://www.nlr.gov/docs/fy21osti/77610.pdf ). This dataset includes the quantity of estimated LDE resource for each hour of the year in 2050, aggregated to both the national level and the ReEDS balancing area level. As described in the accompanying report, the ReEDS model was used to project the evolution of the power sector over time. Links to the mapping of ReEDS balancing areas to states and ReEDS balancing area shape files are provided. This dataset also includes the report figure data.

08 HYDROGEN↗

Availability Considerations in the Design of K-DEMO

A DEMO device has been considered the next step following ITER as a near-term prototypical reactor design that is tritium self-sufficient and produces a limited amount of net electricity. The machine maintenance approach and planned configuration concept plays a major role in establishing the design point. DEMO will also need to show that adequate operating availability can be achieved over a reasonable time period, as a last step before full-scale electricity production. The ability to operate with high availability/reliability plays a key ingredient in defining the DEMO configuration, fostering the need for rapid removal/replacement of limited-life in-vessel components. DEMO pre-conceptual studies are being carried out by South Korea (with US participation) and other countries. The device designs span a range of maintenance approaches from full radial extraction of large in-vessel modules through all TF horizontal openings to vertical maintenance of segmented in-vessel components. Progress made on the S. Korea's K-DEMO design will be provided with emphasis on the design choices identified to promote high availability.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A Study of IR Loss Correction Methodologies for Commercially Available Pyranometers

This presentation provides a high-level overview of a study of IR Loss Connection Methodologies for Commercially Available Pyranometers. The IR Loss Corrections Study is investigating how various correction methodologies work for several makes and models of commercially available pyranometers in common use, both when operated in ventilators with DC fans and without ventilators, as when they are typically calibrated.

14 SOLAR ENERGY↗

Machine Learning for Improved Availability of the SNS Klystron High Voltage Converter Modulators

Beam availability has increased at the SNS, however, the targeted availability is greater than 95 %, while the SNS has failed to meet lower targets in the past. The HVCM used to power the linac klystrons have been one source of lost beam time and was chosen to explore using AI/ML techniques to improve reliability. Among the possibilities being explored are automating the tuning of HVCMs and predicting component failures such as capacitor aging, rectifier assemblies containing hundreds of diodes, and insulating oil degradation. The methodology pursued includes data cleaning, de-noising, post-analysis data labeling, and machine learning model development. We explore using Long Short-Term Memory and autoencoders for anomaly detection and prognostication used to schedule maintenance. We evaluate the use of model regularizers and constraints to improve the performance of the model and investigate methods to estimate the uncertainty of the models to provide a robust prediction with statistical interoperability. This paper describes the operational experience and known failures of the HVCMs and the proposed ML methodology and the preliminary results of training the AI/ML algorithms.

Pappas, Chris↗

The weathered bedrock vadose zone: A hidden control on water availability in the western United States (Final Technical Report)

This report summarizes the research completed during the project entitled "The weathered bedrock vadose zone: A hidden control on water availability in the western United States" (Award: DE-SC0018039). The report covers work completed between 9/15/17 and 9/14/20. The objective of the exploratory project work was to evaluate the overarching hypothesis that the subsurface underlying actively eroding landscapes is systematically structured as a result of a balance between weathering and erosion and that this structure leads to dynamic moisture storage within weathered rock. To accomplish project objectives, we characterized hillslope weathering profile structure and conducted subsurface moisture monitoring across four study sites with comparable bedrock types but differing erosion rate and hydroclimate: The Angelo Coast Range Reserve (ACRR), Sagehorn-Russell Ranch (SRR), Antelope Valley Ranch (AVR), and the LBNL Watershed Function Scientific Focus Area (SFA). New characterization and monitoring infrastructure at the AVR and SFA sites was established through this project and enables long-term monitoring of hydrologic and carbon fluxes at the sites for comparison with long-term monitoring in other sites. Deep drilling, downhole logging, and characterization of chemistry and porosity of rock recovered from drilling reveals two weathering fronts across the sites. A shallower front is marked by the extent of pervasive fracturing and oxidation of pyrite and organic carbon. A deeper weathering front is marked by the appearance of open fractures and discoloration. At the AVR site, multiple ridge tops of different hillslope length were investigated to establish the relationship between the weathering front and topography. Drilling reveals that the fraction of the hillslope relief that is weathered scales with hillslope length, consistent with the theoretical framework that guided the project relating groundwater drainage with landscape evolution. With the establishment of new boreholes, we have documented significant water storage in weathered bedrock using low-field borehole nuclear magnetic resonance (NMR) across all four sites. In the upper 4 m of bedrock, the average water contents at ACRR, AVR, SRR, and SFA are approximately 25%, 16%, 25%, 23% respectively. At the ACRR site, where long-term monitoring via neutron probe surveys and time-domain transmission sensors are available, we have demonstrated that NMR reliably records moisture content and importantly, moisture content dynamics in weathered bedrock. Across all sites, our NMR monitoring has also revealed that a considerable fraction of the water storage occurs in fractures or pores that are significantly larger than the fine-grained bedrock matrix of the parent rock. To identify the proportion of water storage occurring in fractures and large pores, we analyzed the NMR signal by evaluating both the sum of echoes and an inversion of the NMR signal for a distribution of T2 relaxation times. Both methods reveal that at least 20% of the water storage occurs in fractures and larger pores across all sites. To constrain the interpretation of the in-situ field-scale NMR measurements, we compare the NMR response in the field to independent pore-structure information obtained via helium pycnometry. Results support the inference that dynamic seasonal water storage is dominantly restricted to the fracture network, which can reach 7% of the total volume. This dynamic range in water content is comparable to that of some soils, underscoring the need to understand how this region of the Earth System is structured, and functions as a hydrologic reservoir.

58 GEOSCIENCES↗

Machine Learning for Improved Availability of the SNS Klystron High Voltage Converter Modulators

Beam availability has increased at the SNS, however, the targeted availability is greater than 95 %, while the SNS has failed to meet lower targets in the past. The HVCM used to power the linac klystrons have been one source of lost beam time and was chosen to explore using AI/ML techniques to improve reliability. Among the possibilities being explored are automating the tuning of HVCMs and predicting component failures such as capacitor aging, rectifier assemblies containing hundreds of diodes, and insulating oil degradation. The methodology pursued includes data cleaning, de-noising, post-analysis data labeling, and machine learning model development. We explore using Long Short-Term Memory and autoencoders for anomaly detection and prognostication used to schedule maintenance. We evaluate the use of model regularizers and constraints to improve the performance of the model and investigate methods to estimate the uncertainty of the models to provide a robust prediction with statistical interoperability. This paper describes the operational experience and known failures of the HVCMs and the proposed ML methodology and the preliminary results of training the AI/ML algorithms.

Pappas, Chris↗

Adaptation of Commercially Available Robots for Conditioning and Packaging ILW at Sellafield - 18282

The Legacy Ponds and Silos at Sellafield site contain some of the most hazardous Intermediate Level Waste (ILW) in the UK. As part of a multi-billion pound decommissioning programme, this waste will be retrieved and transported across the site for conditioning and packaging in the Box Encapsulation Plant (BEP). Current plans are to commence plant active commissioning in 2022, which will represent a major milestone in the UK nuclear decommissioning programme. Within the plant, skips of waste will be transferred from the shielded transport package into standard 3 cubic metre ILW containers. Waste from the Pile Fuel Storage Pond and First Generation Magnox Storage Pond will be immobilised in cementitious grout before being transferred to an adjacent store. Waste from the Magnox Swarf Storage Silo will not be immobilised in grout, but will be packaged, providing multi-barrier containment. During the concept design, the project team selected commercially available robots as the most viable technology for carrying out the numerous dexterous and repetitive tasks involved in characterising, conditioning and packaging the waste within the shielded 'hot' cells. Significant technical challenges had to be overcome during the design to adapt a commercially available robot for use within a high radiation / high contamination environment, where human access is not safe even for short periods of time. These challenges included: - Designing robot assemblies that could be retrofitted into the existing cell structure that was constructed as part of an earlier project but never completed; - Designing robot assemblies that could be recovered to their 'transport' position without requiring manual intervention; - Designing robot assemblies that could be remotely removed and replaced with new ones if they failed part way through the plant operational life; - Designing through-wall service and power connections that allow maintenance and recovery; - Designing control systems and human-machine interfaces that enable both manual and automated operation of the robot, whilst avoiding collisions with the in-cell cranes and other in cell furniture; - Assessing the radiation tolerance of all items. Bespoke computer simulations and full-scale development trials have been carried out, using non-active test materials, in order to demonstrate the Technology Readiness Level (TRL) required by Sellafield Limited (SL) at each stage of the project [1]. The results of these trials have been recorded in both video and written form so that they can form part of the project's Knowledge Management database. Detailed designs and specifications for the robot assemblies have been updated to incorporate the recommendations from the development trials. Procurement and manufacture of robot assemblies has already commenced to deliver two waste handling robots, two waste container lidding / swabbing robots and associated critical spares. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

The use of GRACE data to monitor natural and anthropogenic induced variations in water availability across Africa

Inter-annual trends in terrestrial water storage (TWS) were extracted from monthly (01/2003–09/2012) Gravity Recovery and Climate Experiment (GRACE) data acquired over Africa and correlated (in a geographic information system [GIS] environment) with relevant temporal remote sensing, geologic, and hydrologic datasets. Findings include the following: (1) large sectors of Africa are undergoing statistically significant TWS variations (+ 44 mm/yr to -15 mm/yr) due to natural and anthropogenic causes; (2) warming of the tropical Atlantic Ocean apparently intensified Atlantic monsoons and increased precipitation and TWS over western and central Africa; (3) warming in the central Indian Ocean decreased precipitation and TWS over eastern Africa; (4) the high frequency of flooding events increased TWS over the Zambezi and Okavango basins; (5) extraction of fossil groundwater decreased TWS over the Saharan aquifers; (6) deforestation decreased TWS in three subbasins (Ubangi, Congo, and Sangha) of the Congo River Basin; and (7) the construction of dams increased TWS in the Blue Nile and Atbara subbasins. Given the 10 years of monthly GRACE data acquired on the subbasin scale across the globe, as well as the plans underway for deployment of a GRACE-FO and GRACE-II, using GRACE-derived TWS data should be considered an alternative, viable index for measuring temporal and spatial variations in water availability.

54 ENVIRONMENTAL SCIENCES↗

Interpreting best available technologies more flexibly: A policy perspective for municipal wastewater management in India and other developing countries

Inadequate sanitation is amongst the causes of escalating pollution problems in developing countries, as municipal wastewater treatment systems remove only a fraction of pollutants that could be removed with best available technologies (BAT). Although BAT is a proven instrument of environmental policies, its potential for municipalities remains largely unused in developing countries. In order to ease its implementation, the paper developed a simplified assessment approach towards identifying an approximating of BAT in terms of a “flexible BAT” (FlexiBAT), which is based on the identification of national reference plants assessed with respect to pollutant removal (environmental impact, health impact), costs (economic viability, affordability) and social acceptability. The concept was tested for 58 case studies in India, where none of the technologies passed all tests for FlexiBAT. Therefore, there is a need to improve or develop better and more innovative technologies. Amongst the most promising ones, membrane bioreactors provided good physical water quality, but costs were high, while for moving bed biofilm reactors costs were low, but water quality was insufficient. Conventional onsite systems require separate consideration. In order to ease the identification of FlexiBAT, a national environmental information system with data from the regular monitoring of existing plants would be needed.

54 ENVIRONMENTAL SCIENCES↗

Using Thermodynamic Availability to Predict the Transitional Film Reynolds Number between the Droplet and Jet Modes in Falling Liquid between Horizontal Tubes

The objective of this paper is to propose and validate a model for predicting the transitional film Reynolds number between the droplet and jet modes of falling film flows. The proposed model relies on thermodynamic availability with no fitting to mode transition data. The model directly predicts the numerical values of the transitional film Reynolds numbers at different operating conditions. The results of the model are validated against 43 experimental data points from the literature, which cover 10 working fluids and a wide range of tube geometries. The accuracy of the proposed model in predicting the validation data is -on average- more than 20 % better than that of the widely used Re = a (Ga) b and Re = a (Ga) b (S/ξ) c models. The model also achieves low computation time (less than 30 seconds for the 43 validation data points). The model presented in this paper has the potential of integration with heat transfer, mass transfer, and pressure drop models for optimizing the design and operation of falling film heat and mass exchangers.

42 ENGINEERING↗

UEMtomaton: A Source-Available Platform to Aid in Start-up of Ultrafast Electron Microscopy Labs

The steady rise in the number of ultrafast electron microscopy (UEM) labs, in addition to the opacity and lack of detailed descriptions of current approaches that would enable point-by-point construction, has created an opportunity for sharing common methods and instrumentation for (for example) automating data acquisition to assist in efficient lab start-up and to learn about common and robust protocols. In the spirit of open sharing of methods, we provide here a description of an entry-level method and user interface (UI) for automating UEM experiments, and we provide access to the source code and scripts (source-available) for ease of implementation or as a starting reference point for those entering or seeking to enter the field (https://github.com/CEMSFlannigan/UEMtomaton/releases/tag/v1.0). Core instrumentation and physical connections in the UEM lab at Minnesota are described. Interface communication schemes consisting of duo server-client pairs between critical components – the optical delay stage and the UEM digital camera – are presented, with emphasis placed on describing the logic and communications sequence designed to conduct automated series acquisitions. An application designed and programmed with C++/CLI as Windows Forms in Microsoft Visual Studio – dubbed UEMtomaton – is also presented. Key to the UI layout is centralization of the automation tasks and establishment of communication within the software rather than by interfacing with each individual workstation. It is our hope that this note provides useful insight for current and future UEM researchers, particularly with respect to generalizability and portability of the approach to emerging labs. Here, we note that while this basic, entry-level approach is certainly not the most sophisticated or comprehensive of those currently in use, we feel there is nevertheless value in clearly communicating a proven straightforward method to hopefully lower the barrier to entry into the field.

47 OTHER INSTRUMENTATION↗

Effects of feedstock availability on the negative ion behavior in a C{sub 4}F{sub 8} inductively coupled plasma

In this paper, the negative ion behavior in a C{sub 4}F{sub 8} inductively coupled plasma (ICP) is investigated using a hybrid model. The model predicts a non-monotonic variation of the total negative ion density with power at low pressure (10–30 mTorr), and this trend agrees well with experiments that were carried out in many fluorocarbon (fc) ICP sources, like C{sub 2}F{sub 6}, CHF{sub 3}, and C{sub 4}F{sub 8}. This behavior is explained by the availability of feedstock C{sub 4}F{sub 8} gas as a source of the negative ions, as well as by the presence of low energy electrons due to vibrational excitation at low power. The maximum of the negative ion density shifts to low power values upon decreasing pressure, because of the more pronounced depletion of C{sub 4}F{sub 8} molecules, and at high pressure (∼50 mTorr), the anion density continuously increases with power, which is similar to fc CCP sources. Furthermore, the negative ion composition is identified in this paper. Our work demonstrates that for a clear understanding of the negative ion behavior in radio frequency C{sub 4}F{sub 8} plasma sources, one needs to take into account many factors, like the attachment characteristics, the anion composition, the spatial profiles, and the reactor configuration. Finally, a detailed comparison of our simulation results with experiments is conducted.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗