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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Macrolide-Peptide Conjugates as Probes of the Path of Travel of the Nascent Peptides through the Ribosome
Abstract not provided
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit
Abstract not provided
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly
Abstract not provided
Quantitative stable isotope probing with H218O reveals that most bacterial taxa in soil synthesize new ribosomal RNA
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A conserved RNA structural motif for organizing topology within picornaviral internal ribosome entry sites
Picornaviral IRES elements are essential for initiating the cap-independent viral translation. However, three-dimensional structures of these elements remain elusive. Here, we report a 2.84-Å resolution crystal structure of hepatitis A virus IRES domain V (dV) in complex with a synthetic antibody fragment—a crystallization chaperone. The RNA adopts a three-way junction structure, topologically organized by an adenine-rich stem-loop motif. Despite no obvious sequence homology, the dV architecture shows a striking similarity to a circularly permuted form of encephalomyocarditis virus J-K domain, suggesting a conserved strategy for organizing the domain architecture. Recurrence of the motif led us to use homology modeling tools to compute a 3-dimensional structure of the corresponding domain of foot-and-mouth disease virus, revealing an analogous domain organizing motif. The topological conservation observed among these IRESs and other viral domains implicates a structured three-way junction as an architectural scaffold to pre-organize helical domains for recruiting the translation initiation machinery.
Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis
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Subdomain dynamics enable chemical chain reactions in non-ribosomal peptide synthetases
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Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance
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An intramolecular macrocyclase in plant ribosomal peptide biosynthesis
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Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridin
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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation. Eight different primer pairs targeting different variable regions of the 16S rRNA gene were employed. DNA sequences were analyzed using open-source platform QIIME2 and the Greengenes database. PICRUSt2 was used to determine the predicted function of bacterial communities. Changes in bacterial relative abundance due to 16S primers were determined by GLMs. The average PCR amplicon size ranged from 315 bp (V3) to 769 bp (V4–V6). Alpha- and beta-diversity, taxonomic composition, and predicted functions were significantly affected by the primer choice. Beta diversity analysis based on Unweighted UniFrac distance matrix showed separation of microbiota with four different clusters of bacterial communities. Based on the alpha- and beta-diversity and taxonomic composition, variable regions V1–V3(1) and (2), and V3–V4 and V3–V5 were in most consensus. Our data strongly suggest that selection of particular sets of the 16S rRNA primers can impact microbiota analysis and interpretation of results in chicken as was shown previously for humans and other animal species.
Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides
Development of R. toruloides as a production host for the sustainable production of the NRP indigoidine.