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Portable telemetry system uses conventional telephone link which eliminates mailing or messenger service between physician and analyst. Transmitter is used by physician; receiver is used by analyst. Each unit is inductively coupled to its respective telephone set, transmitter converting EEG into audio frequency and receiver converting this frequency back to EEG.
It is shown that hypergravity (35g) stimulates the production of inositol 1,4,5-trisphosphate (IP3) and decreases adenosine 3-prime,5-prime-cyclic monophosphate (cAMP) levels in HeLa cells. It is proposed that IP3 and cAMP may act as second messengers in hypergravity signal transduction. Phosphorylation of microtubule-associated proteins in both the detergent-soluble and -insoluble fractions suggests that cytoskeletal structures may be influenced by gravity.
Model systems are available for mechanically stimulating cultured skeletal muscle cells by passive tensile forces which simulate those found in vivo. When applied to embryonic muscle cells in vitro these forces induce tissue organogenesis, metabolic adaptations, and muscle cell growth. The mechanical stimulation of muscle cell growth correlates with stretch-induced increases in the efflux of prostaglandins PGE2 and PGF2(alpha) in a time and frequency dependent manner. These prostaglandins act as mechanical 'second messengers' regulating skeletal muscle protein turnover rates. Since they also effect bone remodelling in response to tissue loading and unloading, secreted prostaglandins may serve as paracrine growth factors, coordinating the growth rates of muscle and bone in response to external mechanical forces. Cell culture model systems will supplement other models in understanding mechanical transduction processes at the molecular level.
This paper loosely summarizes the Symposium in the context of estimating the distance to the solar wind termination shock. Thus the major constituents of the heliosphere are first reviewed including the solar wind and its shocks, magnetic structure and stream structure, the interstellar neutral gas and its daughters, the interstellar pickup ions and the cosmic ray anomalous component, and the galactic cosmic rays. Then a direct calculation of the distance to the termination shock is presented, which yields 80 AU but with large error bars. Finally information carried by messengers from the termination shock and heliosheath (the kHz radio bursts observed by Voyager, galactic cosmic rays, the cosmic ray anomalous component, and the interstellar neutral gas and backscattered photons) is assessed and interpreted. The paper concludes that 80 AU is the best bet.
This investigation was conducted by the National Advisory Committee for Aeronautics at Langley Field, Va., at the request of the Army Air Corps, for the purpose of comparing the full scale lift and drag characteristics of an airplane equipped with several sets of wings of commonly used airfoil sections. A Sperry Messenger Airplane with wings of R.A.F.-15, U.S.A.-5, U.S.A.-27, and Gottingen 387 airfoil sections was flown and the lift and drag characteristics of the airplane with each set of wings were determined by means of glide tests. The results are presented in tabular and curve form. (author)
The Naval Research Laboratory has been involved in developing InP solar cell technology since 1988. The purpose of these programs was to produce advanced cells for use in very high radiation environments, either as a result of operating satellites in the Van Allen belts or for very long duration missions in other orbits. Richard Statler was technical representative on the first program, with Spire Corporation as the contractor, which eventually produced several hundred, high efficiency 2 x 2 sq cm single crystal InP cells. The shallow homojunction technology which was developed in this program enabled cells to be made with AMO, one sun efficiencies greater than 19%. Many of these cells have been flown on space experiments, including PASP Plus, which have confirmed the high radiation resistance of InP cells. NRL has also published widely on the radiation response of these cells and also on radiation-induced defect levels detected by DLTS, especially the work of Rob Walters and Scott Messenger. In 1990 NRL began another Navy-sponsored program with Tim Coutts and Mark Wanlass at the National Renewable Energy Laboratory (NREL), to develop a one sun, two terminal space version of the InP-InGaAs tandem junction cell being investigated at NREL for terrestrial applications. These cells were grown on InP substrates. Several cells with AM0, one sun efficiencies greater than 22% were produced. Two 2 x 2 sq cm cells were incorporated on the STRV lA/B solar cell experiment. These were the only two junction, tandem cells on the STRV experiment. The high cost and relative brittleness of InP wafers meant that if InP cell technology were to become a viable space power source, the superior radiation resistance of InP would have to be combined with a cheaper and more robust substrate. The main technical challenge was to overcome the effect of the dislocations produced by the lattice mismatch at the interface of the two materials. Over the last few years, NRL and Steve Wojtczuk at Spire have been developing a single junction InP on Si cell, in an ONR-sponsored SBIR program. Both cell polarities were investigated and the best efficiencies to date (approximately 13% on a 2 x 4 sq cm cell) were achieved with n/p cells. Earlier this year NRL began a program with ASEC to develop a two terminal InP-InGaAs tandem cell on a Ge substrate. RTI and NREL are subcontractors on this program. The results of an ONR-sponsored study of the potential market for InP/Si cells will be discussed. Also the technical status of both the InP/Si and the InP-InGaAs/Ge programs will be given. The technical challenges still remaining will be briefly described.
A critical factor in the maintenance of bone mass is the physical forces imposed upon the skeleton. Removal of these forces, such as in a weightless environment, results in a rapid loss of bone, whereas application of exogenous mechanical strain has been shown to increase bone formation. Numerous flight and ground-based experiments indicate that the osteoblast is the key bone cell influenced by mechanical stimulation. Aside from early transient fluctuations in response to unloading, osteoclast number and activity seem unaffected by removal of strain. However, bone formation is drastically reduced in weightlessness and osteoblasts respond to mechanical strain with an increase in the activity of a number of second messenger pathways resulting in increased anabolic activity. Unfortunately, the mechanism by which the osteoblast converts physical stimuli into a biochemical message, a process we have termed biochemical coupling, remains elusive. Prior to the application of this grant, we had characterized a mechanosensitive, cation nonselective channel (SA-cat) in osteoblast-like osteosarcoma cells that we proposed is the initial signalling mechanism for mechanotransduction. During the execution of this grant, we have made considerable progress to further characterize this channel as well as to determine its role in the osteoblastic response to mechanical strain. To achieve these goals, we combined electrophysiologic techniques with cellular and molecular biology methods to examine the role of these channels in the normal function of the osteoblast in vitro.
Calcium, a universal second messenger, regulates diverse cellular processes in eukaryotes. Ca-2(+) and Ca-2(+)/calmodulin-regulated protein phosphorylation play a pivotal role in amplifying and diversifying the action of Ca-2(+)- mediated signals. A chimeric Ca-2(+)/calmodulin-dependent protein kinase (CCaMK) gene with a visinin-like Ca-2(+)- binding domain was cloned and characterized from lily. The cDNA clone contains an open reading frame coding for a protein of 520 amino acids. The predicted structure of CCaMK contains a catalytic domain followed by two regulatory domains, a calmodulin-binding domain and a visinin-like Ca-2(+)-binding domain. The amino-terminal region of CCaMK contains all 11 conserved subdomains characteristic of serine/threonine protein kinases. The calmodulin-binding region of CCaMK has high homology (79%) to alpha subunit of mammalian Ca-2(+)/calmodulin-dependent protein kinase. The calmodulin-binding region is fused to a neural visinin-like domain that contains three Ca-2(+)-binding EF-hand motifs and a biotin-binding site. The Escherichia coli-expressed protein (approx. 56 kDa) binds calmodulin in a Ca-2(+)-dependent manner. Furthermore, Ca-45-binding assays revealed that CCaMK directly binds Ca-2(+). The CCaMK gene is preferentially expressed in developing anthers. Southern blot analysis revealed that CCaMK is encoded by a single gene. The structural features of the gene suggest that it has multiple regulatory controls and could play a unique role in Ca-2(+) signaling in plants.
The National Aeronautics and Space Administration (NASA) and, in particular, the Marshall Space Flight Center (MSFC) have played pivotal roles in the advancement of space exploration and space-related science and discovery since the early 1960's. Many of the extraordinary accomplishments and advancements of NASA and MSFC have gone largely unheralded to the general public, though they often border on the miraculous. This lack of suitable and deserved announcement of these "miracles" seems to have occurred because NASA engineers and scientists are inclined to regard extraordinary accomplishment as a normal course of events. The goal in this project has been to determine an effective structure and mechanism for communicating to the general public the extent to which our investment in our US civilian space program, NASA, is, in fact, a very wise investment. The project has involved discerning important messages of truth which beg to be conveyed to the public. It also sought to identify MSFC personnel who are particularly effective as messengers or communicators. A third aspect of the project was to identify particular target audiences who would appreciate knowing the facts about their NASA investment. The intent is to incorporate the results into the formation of an effective, proactive MSFC speakers bureau. A corollary accomplishment for the summer was participation in the formation of an educational outreach program known as Nasa Ambassadors. Nasa Ambassadors are chosen from the participants in the various MSFC summer programs including: Summer Faculty Fellowship Program (SFFP), Science Teacher Enrichment Program (STEP), Community College Enrichment Program (CCEP), Joint Venture (JOVE) program, and the NASA Academy program. NASA Ambassadors agree to make pre-packaged NASA-related presentations to non-academic audiences in their home communities. The packaged presentations were created by a small cadre of participants from the 1996 MSFC summer programs, volunteering their time beyond their normal NASA summer research commitment. A total of eight presentations were created and made available for use by NASA Ambassadors. A major segment of the research effort during the summer has been devoted to verifying and documenting certain "spinoff' contributions of NASA technology and in determining their relevance and impact to our society and our nation's economy. The purpose behind the verification/documentation research has been to shed light on the question of whether or not our NASA investment is a wise investment. It has revealed that NASA is a wise investment.
The purpose of the Space 2000 Symposium is to present the creativity and achievements of key figures of the 20th century. It offers a retrospective discussion on space exploration. It considers the future of the enterprise, and the legacy that will be left for future generations. The symposium includes panel discussions, smaller session meetings with some panelists, exhibits, and displays. The first session entitled "From Science Fiction to Science Facts" commences after a brief overview of the symposium. The panel discussions include talks on space exploration over many decades, and the missions of the millennium to search for life on Mars. The second session, "Risks and Rewards of Human Space Exploration," focuses on the training and health risks that astronauts face on their exploratory mission to space. Session three, "Messages and Messengers Informing and Inspire Space Exploration and the Public," focuses on the use of TV medium by educators and actors to inform and inspire a wide variety of audiences with adventures of space exploration. Session four, "The Legacy of Carl Sagan," discusses the influences made by Sagan to scientific research and the general public. In session five, "Space Exploration for a new Generation," two student speakers and the NASA Administrator Daniel S. Goldin address the group. Session six, "Destiny or Delusion? -- Humankind's Place in the Cosmos," ends the symposium with issues of space exploration and some thought provoking questions. Some of these issues and questions are: what will be the societal implications if we discover the origin of the universe, stars, or life; what will be the impact if scientists find clear evidence of life outside the domains of the Earth; should there be limits to what humans can or should learn; and what visionary steps should space-faring people take now for future generations.
The central dogma of molecular biology states that information is stored in DNA, transcribed to messenger RNA (mRNA) and then translated into proteins. This picture is significantly augmentated when we consider the action of certain proteins in regulating transcription. These transcription factors provide a feedback pathway by which genes can regulate one another's expression as mRNA and then as protein. To review: DNA, RNA and proteins have different functions. DNA is the molecular storehouse of genetic information. When cells divide, the DNA is replicated, so that each daughter cell maintains the same genetic information as the mother cell. RNA acts as a go-between from DNA to proteins. Only a single copy of DNA is present, but multiple copies of the same piece of RNA may be present, allowing cells to make huge amounts of protein. In eukaryotes (organisms with a nucleus), DNA is found in the nucleus only. RNA is copied in the nucleus then translocates(moves) outside the nucleus, where it is transcribed into proteins. Along the way, the RNA may be spliced, i.e., may have pieces cut out. RNA then attaches to ribosomes and is translated to proteins. Proteins are the machinery of the cell other than DNA and RNA, all the complex molecules of the cell are proteins. Proteins are specialized machines, each of which fulfills its own task, which may be transporting oxygen, catalyzing reactions, or responding to extracellular signals, just to name a few. One of the more interesting functions a protein may have is binding directly or indirectly to DNA to perform transcriptional regulation, thus forming a closed feedback loop of gene regulation. The structure of DNA and the central dogma were understood in the 50s; in the early 80s it became possible to make arbitrary modifications to DNA and use cellular machinery to transcribe and translate the resulting genes; more recently, genomes (i.e., the complete DNA sequence) of many organisms have been sequenced. This large-scale sequencing began with simple organisms, viruses and bacteria, progressed to eukaryotes such as yeast, and more recently (1998) progressed to a multi-cellular animal, the nematode Caenorhabditis elegans. Sequencers have now moved on to the fruit fly Drosophila melanogaster, whose sequence is slated for completion by the end of 1999. The human genome project is expected to determine the complete sequence of all 3 billion bases of human DNA within the next five years. In the wake of genome-scale sequencing, further instrumentation is being developed to assay gene expression and function on a comparably large scale. Much of the work in computational biology focuses on computational tools used in sequencing, finding genes that are related to a particular gene, finding which parts of the DNA code for proteins and which do not, understanding what proteins will be formed from a given length of DNA, predicting how the proteins will fold from a one-dimensional structure into a three dimensional structure, and so on. Much less computational work has been done regarding the function of proteins. One reason for this is that different proteins function very differently, and so work on protein function is very specific to certain classes of proteins. There are, for example, proteins such enzymes that catalyze various intracellular reactions, receptors that respond to extracellular signals and ion channels that regulate the flow of charged particles into and out of the cell. In this chapter, we will consider a particular class of proteins called transcription factors(TFs), which are responsible for regulating when a certain gene is expressed in a certain cell, which cells it is express in, and how much is expressed. Understanding these processes will involve developing a deeper understanding of transcription, translation, and the cellular processes that control those processes. All of these elements fall under the aegis of gene regulation or more narrowly transcriptional regulation. Some of the key questions in gene regulation are: What genes are expressed in a certain cell at a certain time? How does gene expression differ from cell to cell in a multicellular organism? Which proteins act as transcription factors, i.e., are important in regulating gene expression? From questions like these, we hope to understand which genes are important for various macroscopic processes. Nearly all of the cells of a multicellular organism contain the same DNA. Yet this same genetic information yields a large number of different cell types. The fundamental difference between a neuron and a liver cell, for example, is which genes are expressed. Thus understanding gene regulation is an important step in understanding development. Furthermore, understanding the usual genes that are expressed in cells may give important clues about various diseases. Some diseases, such as sickle cell anemia and cystic fibrosis, are caused by defects in single, non-regulatory genes; others, such as certain cancers, are caused when the cellular control circuitry malfunctions - an understanding of these diseases will involve pathways of multiple interacting gene products. There are numerous challenges in the area of understanding and modeling gene regulation. First and foremost, biologists would like to develop a deeper understanding of the processes involved, including which genes and families of genes are important, how they interact, etc. From a computation point of view, there has been embarrassingly little work done. In this chapter there are many areas in which we can phrase meaningful, non-trivial computational questions, but questions that have not been addressed. Some of these are purely computational (what is a good algorithm for dealing with a model of type X) and others are more mathematical (given a system with certain characteristics, what sort of model can one use? How does one find biochemical parameters from system-level behavior using as few experiments as possible?). In addition to biological and algorithmic problems, there is also the ever-present issue of theoretical biology - what general principles can be derived from these systems, what can one do with models other than just simulate time-courses, what can be deduced about a class of systems without knowing all the details? The fundamental challenge to computationalists and theorists is to add value to the biology - to use models, modeling techniques and algorithms to understand the biology in new ways.
One of the most intriguing scientific questions about Mercury is whether the planet possesses a liquid core. One way that this can be ascertained is through the measurement of the longitudinal libration of Mercury about its rotation axis. The MESSENGER (Mercury Surface, Space, Environment, Geochemistry, and Ranging) mission to Mercury will attempt this measurement by deriving the rotation of the planet from remotely sensed observations of the Mercury gravity field and the planet's shape. For a fully fluid core the libration is expected to be an approximately 350-m-amplitude oscillation (at the equator) about the mean rotation of the planet. Thus, the shape of the planet undergoes a librational oscillation in the same way that features on the surface participate in the motion. In this sense altimetric topography can be considered analogous to imaging, with the important exception that for altimetry, the long wavelengths corresponding to low degrees and orders in a spherical harmonic expansion, can be precisely determined from orbital measurements. The rotation of the planet thus introduces a time dependence of the topographic image in an inertial (i.e. fixed) reference frame and the libration is a variation in the planetary rotation rate, manifest as an oscillation of the topographic image. Similarly, gravity measures the distribution of mass within a planet and as such can be viewed as providing a vertically integrated 'image' of the internal density structure. For a librating planet the gravitational field will also reveal a variation in the rotation rate, manifest as an oscillation of the field with respect to an inertial reference frame. While the long-wavelength gravity field contains contributions from the radial distribution of mass with depth, the long wavelength terms are most sensitive to the mass distribution of the deep interior. Thus, both the topography and the gravity independently can be used to determine the (irregular) rotation of a planet, although the topographical method measures the rotation of the crust while the gravity measures the rotation of the distributed mass, and these are not necessarily identical. For example, for a planet that contains a fluid outer core that is effectively decoupled from the mantle the gravity field will not librate in the manner of a solid body. The libration obtained from the variable rotation of the gravity field will represent a combination of contributions due to a librating mantle and a differentially rotating fluid core. Differential rotation of the Earth's inner core had been theoretically predicted from three dimensional geodynamical models and subsequently reported from seismic observations. A difference between the libration of the lithosphere as determined from altimetry and the deep interior as determined from gravity, if it could be detected, might provide further insight into the nature of core-mantle coupling. Additional information is contained in the original extended abstract.
We review past assertions that the determinations of the four parameters, C(20), C(22), theta, phi, are sufficient to determine the size and state of Mercury's core. C(20) and C(22) are gravitational harmonics, theta is Mercury's obliquity and phi is the amplitude of the forced, 88 day period libration in longitude. The upcoming MESSENGER orbiter mission to Mercury with onboard instrumentation capable of measuring these four parameters, and the possibility of precision measurements of Mercury's spin geometry with radar interferometry techniques make a reexamination of this proposal particularly relevant. The two necessary conditions on the core-mantle interaction for the experiment to work are: 1. The core must not follow the 88 day physical librations of the mantle. 2. The core must follow the mantle on the time scale of the 250,000 year precession of the spin in Cassini state 1. We shall assume these two conditions are satisfied to develop the method and later establish the constraints on the core viscosity for which they are satisfied. Proposed mechanisms of core mantle coupling other than a viscous coupling do not frustrate the first condition. The physical libration of the mantle about the mean resonant angular velocity arises from the periodically reversing torque on the permanent deformation as Mercury rotates relative to the Sun. Additional information is contained in the original extended abstract.
The grant focuses on a gene we identified called, serum and glucocorticoid- induced kinase (SGK), during a previously funded NASA project. The abundance of SGK messenger RNA (mRNA) and protein is increased in CNS tissues from animals reared in microgravity in comparison with 1G reared animals. In the funded proposal we had three aims: 1) characterize the distribution of SGK mRNA in the developing and adult rat CNS, 2) determine if expression of enzymatically active or inactive forms of SGK in cells influenced cell morphology (neurite growth), and 2) determine if SGK is a CREB kinase - that is, a protein kinase that adds phosphate groups to the transcription factor CREB. Over the past year we have made strong progress in the two most difficult parts of the project, namely specific aims 2 and 3. In specific aim #2 we planned to express a dominant negative or a constitutively active form of SGK in PC12 cells and assay the effects on neurite growth. Several methods are available for examining the effects of a transgene on PC12 neurite growth. Relevant variables include the performance of the assay +/- serum, +/- NGF, substratum for growth, timing between transfection and assay. Over the past 8 months we have customized the assay to enable us to most readily determine the effects of transgene expression on neurite growth. We have also compared the relative utility of transfecting DNA as opposed to protein itself. We are now well positioned to study the effects of SGK on neurite growth. We have also made progress in parallel studies in primary neurons. We have made constructs which will lead to transgene expression in cultures of spinal cord neurons. Co-transfection of a reporter and the SGK constructs can now be performed.
Contents include a summary of publications followed by their abstracts titeled: 1. On microlensing rates and optical depth toward the Galactic center. 2. Newly discovered brown dwarfs not seen in microlensing timescale frequency distribution? 3. Origin and evolution of the natural satellites. 4. Probing the structure of the galaxy with microlensing. 5. Tides, Encyclopedia of Astronomy and Astrophysics. 6. The Puzzle of the Titan-Hyperion 4:3 Orbital Resonance. 7. On the Validity of the Coagulation Equation and the Nature of Runaway Growth. 8. Making Hyperion. 9. The MESSENGER mission to Mercury: Scientific objectives and implementation. 10. A Survey of Numerical Solutions to the Coagulation. 11. Probability of detecting a planetary companion during a microlensing event. 12. Dynamics and origin of the 2:l orbital resonances of the GJ876 planets. 13. Planetary Interior Structure Revealed by Spin Dynamics. 14. A primordial origin of the Laplace relation among the Galilean Satellites. 15. A procedure for determining the nature of Mercury's core. 16. Secular evolution of hierarchical planetary systems. 17. Tidally induced volcanism. 18. Extrasolar planets and mean motion resonances. 19. Comparison of a ground-based microlensing search for planets with a search from space.
Grain size separate CG from the Murray CM2 carbonaceous chondrite contains mostly spinel grains of average diameter 0.5 m. Zinner et al. found that approximately 1% of these spinel grains are of presolar origin as determined by their large O isotopic anomalies. These O isotopic measurements were made with the NanoSIMS on individual grains that were well separated from one another on a gold foil. The grains were selected for analysis from secondary electron and secondary O-16(-) images. The primary beam was then successively deflected onto these single grains for O isotopic analysis. While single grain analysis on dispersed samples is effective for finding relatively abundant anomalous grains, ion imaging in a raster mode on tightly packed grains might be more efficient for locating few anomalous grains among predominantly isotopically normal grains. In fact, this was the analysis mode used by Messenger et al. to discover presolar silicates in interplanetary dust particles. In an exploratory effort that is also geared toward establishing the optimum isotopic imaging technique in the search for presolar silicate grains in primitive meteorites, we measured O isotopic ratios in spinel grains from the Murray CG separate by raster imaging of areas with more or less tightly packed grains.
Hydrogen isotopes are highly variable among primitive meteorites and interplanetary dust particles (IDPs) [1, 2]. In particular, many primitive objects exhibit D (and N-15) enrichments, relative to terrestrial values, thought to represent partial preservation of presolar material fractionated in molecular clouds. However, the diversity of D/H ratios among IDPs and chondrites indicates a complex history of processing in the solar nebula and on meteorite parent bodies. Deconvolving this record requires identification and characterization of the carriers of D enrichments in different objects. Isotopic imaging has proven to be a powerful method to quantitatively explore the distribution of D/H ratios on a one to several m scale in IDPs [2-4] and the CR chondrite Renazzo [5, 6]. In this study, we have used ion imaging to explore the microscale D/H distribution of two carbonaceous chondrites, Tagish Lake (unique) and Al Rais (CR2). Previous D/H measurements (on a ~tens of microns scale) of Tagish Lake matrix fragments by Messenger [7] and Engrand et al. [8] have found different results, most likely related to the analytical techniques used. Previous work has also shown a large range of D/H ratios in CR chondrites, including very large variations on a scale of a few microns [5, 6, 9].
NASA is studying advanced technologies for a future robotic exploration mission to the asteroid belt. The prospective ANTS (Autonomous Nano Technology Swarm) mission comprises autonomous agents including worker agents (small spacecra3) designed to cooperate in asteroid exploration under the overall authoriq of at least one ruler agent (a larger spacecraft) whose goal is to cause science data to be returned to Earth. The ANTS team (ruler plus workers and messenger agents), but not necessarily any individual on the team, will exhibit behaviors that qualify it as an autonomic system, where an autonomic system is defined as a system that self-reconfigures, self-optimizes, self-heals, and self-protects. Autonomic system concepts lead naturally to realistic, scalable architectures rich in capabilities and behaviors. In-depth consideration of a major mission like ANTS in terms of autonomic systems brings new insights into alternative definitions of autonomic behavior. This paper gives an overview of the ANTS mission and discusses the autonomic properties of the mission.