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At least 235 records · Page 13

Comparison of Genotoxic Damage in Monolayer Cell Cultures and Three-Dimensional Tissue-Like Cell Assemblies

Assessing the biological risks associated with exposure to the high-energy charged particles encountered in space is essential for the success of long-term space exploration. Although prokaryotic and eukaryotic cell models developed in our laboratory and others have advanced our understanding of many aspects of genotoxicity, in vitro models are needed to assess the risk to humans from space radiation insults. Such models must be representative of the cellular interactions present in tissues and capable of quantifying I genotoxic damage. Toward this overall goal, the objectives of this study were to examine the effect of the localized microenvironment of cells, cultured as either 2-dimensional (2D) monolayers or 3-dimensional (3D) aggregates, on the rate and type of genotoxic damage resulting from exposure to iron charged particles, a significant portion of space radiation. We used rodent transgenic cell lines containing 50-70 copies of a LacI transgene to provide the enhanced sensitivity required to quantify mutational frequency and type in the 1,100-bp LacI target as well as assessment of DNA,damage to the entire 45-kbp construct. Cultured cells were exposed to high-ener~ir on charged particles at Brookhaven National Laboratory s Alternating Gradient Synchrotron facility for a total dose of 0, 0.1, 0.25,0.5, 1.0, or 2.0 Gy and allowed to recover for 0, 1, or 7 days, after which mutational type and frequency were evaluated. The mutational frequency was found to be higher in 3D samples than in 2D samples at all radiation doses. Mutational frequency also was higher at 7 days after irradiation than immediately after exposure. DNA sequencing of the mutant targets revealed that deletional mutations contributed an increasingly high percentage (up to 27%) of all mutations in cells as the dose was increased from 0.5 to 2 Gy. Several mutants also showed large and complex deletions in multiple locations within the Lac1 target. However, no differences in mutational type were found between the 2D and the 3D samples. These 3D tissue-like model systems can reduce the uncertainty involved in extrapolating risk between in vitro cellular and in vivo models.

Behravesh, E.↗

Using Principal Component Analysis (PCA) to Speed up Radiative Transfer (RT) Computations

Multiple scattering RT calculations time-consuming. Need a speed improvement of about 1000 (for OCO)! Solution: Make use of redundancies in spectra. Correlated-k (Lacis and Wang, Lacis and Oinas, Goody et al, Fu and Liou) Problem: Assume that spectral variation of atmospheric optical properties spatially correlated at all points along optical path. High accuracy (HI) and 2-stream (2S) calculations have high correlation. Single scattering (SS) computations highly scenario-dependent, but not time consuming. Perform SS and 2S calculations at every wavelength. Perform small number of HI computations. Need to compute correction factor B at every wavelength.

data sets↗

The nature of the central parsec of the Galaxy

Observations of infrared fine-structure line emission from compact clouds of ionized gas in the galactic center have been reported by Lacy et al (1979, 1980). These observations suggest the existence of a central black hole of nearly 3,000,000 solar masses and require mechanisms to generate, ionize, and dispose of the gas clouds. It is found that the best model to fulfill these requirements involves cloud generation through disruption of red giants by stellar collisions, ionization by a population of stars which is affected either by enhanced metal abundances or the death of the most massive stars, and gas disposal by star formation. Although the existence of a massive black hole cannot be ruled out, it would play no necessary role in this model and may cause the tidal disruption of stars at a rate such that their accretion into the black hole would produce more radiation than is observed.

Lacy, J. H.↗

Radiative forcing of climate by changes in the vertical distribution of ozone

Results from a one-dimensional radiative-convective equilibrium model (similar to that of Lacis et al., 1981) of surface-temperature changes induced by changes in the vertical distribution of O3 in the atmosphere are parameterized and applied to measurement data for the northern midlatitudes during the 1970s. The construction of the model is outlined, and the results are presented in tables and graphs and discussed in detail. It is found that decreases in O3 in the lower stratosphere in the 1970s caused atmospheric cooling greater than that due to CO2 (thus possibly obscuring the CO2 greenhouse effect) and surface cooling which outweighed the warming effect of increased O3 in the troposphere. The net surface cooling is shown to be equal to about half of the estimated CO2 warming effect for the period.

Lacis, Andrew A.↗

TERSSE. Definition of the total earth resources system for the shuttle era. Volume 10: (TOSS) TERSSE operational system study

Economic benefits studies regarding the application of remote sensing to resource management and the Total Earth Resources for the Shuttle Era (TERSSE) study to outline the structure and development of future systems are used, along with experience from LANDSAT and LACIE, to define the system performance and economics of an operational Earth Resources system. The system is to be based on current (LANDSAT follow-on) technology and its application to high priority resource management missions, such as global crop inventory. The TERSSE Operational System Study (TOSS) investigated system-level design alternatives using economic performance as the evaluation criterion. As such, the TOSS effort represented a significant step forward in the systems engineering and economic analysis of Earth Resources programs. By parametrically relating engineering design parameters, such as sensor performance details, to the economic benefit mechanisms a new level of confidence in the conclusions concerning the implementation of such systems can be reached.

Stow, W. K.↗

Single-class classification

Often, when classifying multispectral data, only one class or crop is of interest, such as wheat in the Large Area Crop Inventory Experiment (LACIE). Usual procedures for designing a Bayes classifier require that labeled training samples and therefore ground truth be available for the 'class of interest' plus all confusion classes defined by the multispectral data. This paper will consider the problem of designing a two-class Bayes classifier which will classify data into the 'class of interest' or the 'other' classes but will require only labeled training samples from the 'class of interest' to design the classifier. Thus, this classifier minimizes the need for ground truth. For these reasons, the classifier is referred to as a single-class classifier. A procedure for evaluating the overall performance of the single-class classifier in terms of the probability of error will be discussed.

Minter, T. C.↗

The Large Area Crop Inventory Experiment /LACIE/ - An assessment after one year of operation

A Large Area Crop Inventory Experiment (LACIE) has been undertaken jointly by the U.S. Department of Agriculture (USDA), the National Oceanic and Atmospheric Administration (NOAA) of the Department of Commerce and the National Aeronautics and Space Administration (NASA) to prove out an economically important application of remote sensing from space. The first phase of the Experiment, which focused upon determinations of wheat area in the U.S. Great Plains and upon the development and testing of yield models, is now nearing completion. The system implemented to handle and analyze the Landsat and meteorological data has generally worked well and met operational goals. A very preliminary assessment of results to date indicates that the accuracy goals of the experiment can be met.

Macdonald, R. B.↗

Wheat signature modeling and analysis for improved training statistics: Supplement. Simulated LANDSAT wheat radiances and radiance components

Simulated scanner system data values generated in support of LACIE (Large Area Crop Inventory Experiment) research and development efforts are presented. Synthetic inband (LANDSAT) wheat radiances and radiance components were computed and are presented for various wheat canopy and atmospheric conditions and scanner view geometries. Values include: (1) inband bidirectional reflectances for seven stages of wheat crop growth; (2) inband atmospheric features; and (3) inband radiances corresponding to the various combinations of wheat canopy and atmospheric conditions. Analyses of these data values are presented in the main report.

Malila, W. A.↗

Investigation of spatial misregistration effects in multispectral scanner data

The author has identified the following significant results. A model for estimating the expected proportion of multiclass pixels in a scene was generalized and extended to include misregistration effects. Another substantial effort was the development of a simulation model to generate signatures to represent the distributions of signals from misregistered multiclass pixels, based on single class signatures. Spatial misregistration causes an increase in the proportion of multiclass pixels in a scene and a decorrelation between signals in misregistered data channels. The multiclass pixel proportion estimation model indicated that this proportion is strongly dependent on the pixel perimeter and on the ratio of the total perimeter of the fields in the scene to the area of the scene. Test results indicated that expected values computed with this model were similar to empirical measurements made of this proportion in four LACIE data segments.

Nalepka, R. F.↗

Evaluation of algorithms for estimating wheat acreage from multispectral scanner data

The author has identified the following significant results. Fourteen different classification algorithms were tested for their ability to estimate the proportion of wheat in an area. For some algorithms, accuracy of classification in field centers was observed. The data base consisted of ground truth and LANDSAT data from 55 sections (1 x 1 mile) from five LACIE intensive test sites in Kansas and Texas. Signatures obtained from training fields selected at random from the ground truth were generally representative of the data distribution patterns. LIMMIX, an algorithm that chooses a pure signature when the data point is close enough to a signature mean and otherwise chooses the best mixture of a pair of signatures, reduced the average absolute error to 6.1% and the bias to 1.0%. QRULE run with a null test achieved a similar reduction.

Nalepka, R. F.↗

System for analysis of LANDSAT agricultural data: Automatic computer-assisted proportion estimation of local areas

The author has identified the following significant results. A conceptual man machine system framework was created for a large scale agricultural remote sensing system. The system is based on and can grow out of the local recognition mode of LACIE, through a gradual transition wherein computer support functions supplement and replace AI functions. Local proportion estimation functions are broken into two broad classes: (1) organization of the data within the sample segment; and (2) identification of the fields or groups of fields in the sample segment.

Nalepka, R. F.↗

Signature extension for spectral variation in soils, volume 4

The reduced 1975-1976 field data at Garden City, Kansas are presented. These data are being used to evaluate the SRVC model predictions, to compare the ERIM-SUITS model with both the SRVC results and field data, and finally, to provide a data base for reviewing multitemporal trajectories. In particular, the applicability of the tasselled cap transformation is reviewed. The first detailed verification of this approach utilizing actual field measured data from the LACIE field measurement program, rather than LANDSAT data, is given.

Berry, J. K.↗

Economic Benefits of Improved Information on Worldwide Crop Production: An Optimal Decision Model of Production and Distribution with Application to Wheat, Corn, and Soybeans

An optimal decision model of crop production, trade, and storage was developed for use in estimating the economic consequences of improved forecasts and estimates of worldwide crop production. The model extends earlier distribution benefits models to include production effects as well. Application to improved information systems meeting the goals set in the large area crop inventory experiment (LACIE) indicates annual benefits to the United States of $200 to $250 million for wheat, $50 to $100 million for corn, and $6 to $11 million for soybeans, using conservative assumptions on expected LANDSAT system performance.

Andrews, J.↗

A distribution benefits model for improved information on worldwide crop production. Volume 1: Model structure and application to wheat

The improved model is suitable for the study of benefits of worldwide information on a variety of crops. Application to the previously studied case of worldwide wheat production shows that about $108 million per year of distribution benefits to the United States would be achieved by a satellite-based wheat information system meeting the goals of LACIE. The model also indicates that improved information alone will not change world stock levels unless production itself is stabilized. The United States benefits mentioned above are associated with the reduction of price fluctuations within the year and the more effective use of international trade to balance supply and demand. Price fluctuations from year to year would be reduced only if production variability were itself reduced.

Andrews, J.↗

The large area crop inventory experiment - A major demonstration of space remote sensing

The NASA-U.S. Department of Agriculture Large Area Crop Inventory Experiment (LACIE), aimed at using multispectral remote sensing data from Landsat 1 and 2 to generate accurate annual global crop production forecasts, is discussed. The forecasts take into account meteorological conditions as well as yield and acreage, and may be used to increase the discrimination of U.S. harvest estimates down to regional levels and to provide more accurate early-season predictions. Sample problems involving the determination of wheat harvests and the monitoring of drought conditions are described. Difficulties related to misidentification of abnormally-developing plantations, the automatic classification of homogeneous spectral groups, the computerized generation of colored maps, and the estimation of yields during years when exceptional meteorological conditions prevail are also considered. Samples of Landsat-generated classification maps for Western U.S. and for the Saratov, U.S.S.R. crop regions are given.

Macdonald, R. B.↗

An evaluation of the signature extension approach to large area crop inventories utilizing space image data

The author has identified the following significant results. Two examples of haze correction algorithms were tested: CROP-A and XSTAR. The CROP-A was tested in a unitemporal mode on data collected in 1973-74 over ten sample segments in Kansas. Because of the uniformly low level of haze present in these segments, no conclusion could be reached about CROP-A's ability to compensate for haze. It was noted, however, that in some cases CROP-A made serious errors which actually degraded classification performance. The haze correction algorithm XSTAR was tested in a multitemporal mode on 1975-76 LACIE sample segment data over 23 blind sites in Kansas and 18 sample segments in North Dakota, providing wide range of haze levels and other conditions for algorithm evaluation. It was found that this algorithm substantially improved signature extension classification accuracy when a sum-of-likelihoods classifier was used with an alien rejection threshold.

Nalepka, R. F.↗

Agricultural scene understanding

The author has identified the following significant results. The LACIE field measurement data were radiometrically calibrated. Calibration enabled valid comparisons of measurements from different dates, sensors, and/or locations. Thermal band canopy results included: (1) Wind velocity had a significant influence on the overhead radiance temperature and the effect was quantized. Biomass and soil temperatures, temperature gradient, and canopy geometry were altered. (2) Temperature gradient was a function of wind velocity. (3) Temperature gradient of the wheat canopy was relatively constant during the day. (4) The laser technique provided good quality geometric characterization.

Landgrebe, D. A.↗