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At least 235 records · Page 13

Feed status and skin injury modulate immunopathology, global gene expression, and survival in channel catfish during virulent Aeromonas hydrophila infection

Introduction VirulentAeromonas hydrophilais a major pathogen in channel catfish (Ictalurus punctatus), that causes motileAeromonassepticemia and significant economic losses. We investigated the effect of feeding status and skin integrity on the host immune response, disease survival, and gastrointestinal pathology following a vAh challenge. Methods Using a bath immersion model, channel catfish were divided into four treatment groups: fin clipped and fed (FCF), fin clipped but not fed (FCN), not fin clipped but fed (NCF), and not fin clipped nor fed (NCN) alongside non-challenged control groups The FCF and NCF groups were fed 2 h prior to the challenge, but the FCN and NCN groups were not. Survival analysis, histopathological assessment, and RNA sequencing were conducted across groups at different time intervals throughout the vAh challenge. Results Survival rates were lowest in the FCF and FCN groups (30% and 23% survival, respectively), suggesting that both feeding and skin damage contributed to disease severity. Histopathological analyses revealed more severe intestinal and gastric lesions in fed groups, characterized by epithelial necrosis, hemorrhage, and edema. Transcriptomic analysis among the groups identified significant differentially expressed genes associated with inflammation, apoptosis, and metabolic stress, with notable upregulation of interleukin 1-beta (il-1β), and complement C3 (c3). Gene ontology enrichment highlighted distinct immune activation patterns between fed and unfed groups, with enhanced pathogen recognition and pro-inflammatory responses in unfed fish. Discussion These findings suggest feeding prior to infection may exacerbate disease pathology, potentially by creating a physiological state conducive to facilitate pathogen proliferation and dampened early immune responses, whereas short-term fasting appears to promote early immune activation. This study provides novel insights into the complex interplay between feed status, physical injury, and immune response to vAh infection.

Immunology↗

Shed Light in the DaRk LineagES of the Fungal Tree of Life—STRES

The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural and anthropogenic habitats. Partly because of their dark, melanin-based pigmentation, black fungi are resistant to stresses including UV- and ionizing-radiation, heat and desiccation, toxic metals, and organic pollutants. Consequently, they are amongst the most stunning extremophiles and poly-extreme-tolerant organisms on Earth. Even though ca. 60 black fungal genomes have been sequenced to date, [mostly in the family Herpotrichiellaceae (Eurotiomycetes)], the class Dothideomycetes that hosts the largest majority of extremophiles has only been sparsely sampled. By sequencing up to 92 species that will become reference genomes, the “Shed light in The daRk lineagES of the fungal tree of life” (STRES) project will cover a broad collection of black fungal diversity spread throughout the Fungal Tree of Life. Interestingly, the STRES project will focus on mostly unsampled genera that display different ecologies and life-styles (e.g., ant- and lichen-associated fungi, rock-inhabiting fungi, etc.). With a resequencing strategy of 10- to 15-fold depth coverage of up to ~550 strains, numerous new reference genomes will be established. To identify metabolites and functional processes, these new genomic resources will be enriched with metabolomics analyses coupled with transcriptomics experiments on selected species under various stress conditions (salinity, dryness, UV radiation, oligotrophy). The data acquired will serve as a reference and foundation for establishing an encyclopedic database for fungal metagenomics as well as the biology, evolution, and ecology of the fungi in extreme environments.

59 BASIC BIOLOGICAL SCIENCES↗

Global Carbon Budget 2025

Accurate assessment of anthropogenic carbon dioxide (CO 2 ) emissions and their redistribution among the atmosphere, ocean, and terrestrial biosphere in a changing climate is critical to better understand the global carbon cycle, support the development of climate policies, and project future climate change. Here we describe and synthesise datasets and methodologies to quantify the five major components of the global carbon budget and their uncertainties. Fossil CO 2 emissions (E FOS ) are based on energy and cement production data. Emissions from land-use change (E LUC ) are estimated by bookkeeping models based on land-use data. The global atmospheric CO 2 growth rate (G ATM ) is computed from changes in concentration measured at surface stations. The global net uptake of CO 2 by the ocean (S OCEAN ) is estimated with global ocean biogeochemistry models and observation-based fCO 2 -products. The global net uptake of CO 2 by the land (S LAND ) is estimated with dynamic global vegetation models. Additional lines of evidence are provided by atmospheric inversions, atmospheric oxygen measurements, ocean interior observation-based estimates, and Earth System Models. This year, we introduced corrections on the E LUC , S OCEAN and S LAND estimates. The sum of all sources and sinks results in the carbon budget imbalance (B IM ), a measure of imperfect data and incomplete understanding of the contemporary carbon cycle. All uncertainties are reported as ± 1σ. For the year 2024, E FOS increased by 1.1 % relative to 2023, with fossil emissions at 10.3 ± 0.5 GtC yr −1 (including the cement carbonation sink, 0.2 GtC yr −1 ), E LUC was 1.3 ± 0.7 GtC yr −1 , for total anthropogenic CO 2 emissions of 11.6 ± 0.9 GtC yr −1 (42.4 ± 3.2 GtCO2 yr −1 ). Also, for 2024, G ATM was 7.9 ± 0.2 GtC yr−1 (3.73 ± 0.1 ppm yr −1 ), 2.2 GtC above the 2023 growth rate. SOCEAN was 3.4 ± 0.4 GtC yr −1 and S LAND was 1.9 ± 1.1 GtC yr −1 , leaving a large negative B IM (−1.7 GtC yr −1 ), suggesting that the total sink or G ATM is strongly overestimated in 2024. The global atmospheric CO 2 concentration averaged over 2024 reached 422.8 ± 0.1 ppm. Preliminary data for 2025 suggest an increase in E FOS relative to 2024 of +1.0 % (0.2 % to 1.7 %) globally, and atmospheric CO 2 concentration increasing by 2.1 ppm reaching 425.6 ppm, 53 % above the pre-industrial level (around 278 ppm in 1750). Overall, the mean and trend in the components of the global carbon budget are consistently estimated over the period 1959–2024, with a near-zero overall budget imbalance, although discrepancies of up to around 1 GtC yr −1 persist for the representation of annual to decadal variability in CO 2 fluxes. Comparison of estimates from multiple approaches and observations shows: (1) a persistent large uncertainty in the estimate of land-use change emissions, (2) a low agreement between the different methods on the magnitude of the land CO 2 flux in the northern extra-tropics, and (3) a discrepancy between the different methods on the mean ocean sink. This living data update documents changes in methods and datasets applied to this most-recent global carbon budget as well as evolving community understanding of the global carbon cycle. The data presented in this work are available at https://doi.org/10.18160/GCP-2025 (Friedlingstein et al., 2025c).

54 ENVIRONMENTAL SCIENCES↗

Solution of large linear systems of equations on the massively parallel processor

The Massively Parallel Processor (MPP) was designed as a special machine for specific applications in image processing. As a parallel machine, with a large number of processors that can be reconfigured in different combinations it is also applicable to other problems that require a large number of processors. The solution of linear systems of equations on the MPP is investigated. The solution times achieved are compared to those obtained with a serial machine and the performance of the MPP is discussed.

Ida, Nathan↗

Precipitation estimation using passive microwave radiometry at 92 and 183 GHz - Aircraft results

The applications of satellite and aircraft sensors to precipitation estimation are discussed. The advanced microwave moisture sensor and imagery and the PPI photographs and digital radar data are described. The aircraft microwave sensor and radar imagery representing the relationships between patterns and gradients of brightness temperature, T(B), and echo intensities for three cases (evolution of an oceanic squall line, convection over land, and intense thunderstorms over land) are examined and compared. The observed T(B)s are also compared with the theoretical calculations of Wu and Weinman (1984) and Szejwach et al. (1986). The observations of convective precipitation from an aircraft microwave radiometer operating at 92 and 183 GHz reveal that the areas of T(B) much colder than atmospheric temperature are positively correlated with regions of higher radar reflectivity, and the patterns and gradients of T(B) are similar in appearance to the radar echoes.

Hakkarinen, Ida M.↗

Solving time-dependent two-dimensional eddy current problems

Results of transient eddy current calculations are reported. For simplicity, a two-dimensional transverse magnetic field which is incident on an infinitely long conductor is considered. The conductor is assumed to be a good but not perfect conductor. The resulting problem is an interface initial boundary value problem with the boundary of the conductor being the interface. A finite difference method is used to march the solution explicitly in time. The method is shown. Treatment of appropriate radiation conditions is given special consideration. Results are validated with approximate analytic solutions. Two stringent test cases of high and low frequency incident waves are considered to validate the results.

Lee, Min Eig↗

A self-consistency approach to improve microwave rainfall rate estimation from space

A multichannel statistical approach is used to retrieve rainfall rates from the brightness temperature T(B) observed by passive microwave radiometers flown on a high-altitude NASA aircraft. T(B) statistics are based upon data generated by a cloud radiative model. This model simulates variabilities in the underlying geophysical parameters of interest, and computes their associated T(B) in each of the available channels. By further imposing the requirement that the observed T(B) agree with the T(B) values corresponding to the retrieved parameters through the cloud radiative transfer model, the results can be made to agree quite well with coincident radar-derived rainfall rates. Some information regarding the cloud vertical structure is also obtained by such an added requirement. The applicability of this technique to satellite retrievals is also investigated. Data which might be observed by satellite-borne radiometers, including the effects of nonuniformly filled footprints, are simulated by the cloud radiative model for this purpose.

Kummerow, Christian↗

Passive microwave retrievals of rainfall and rain system structure

Consideration is given to the development of algorithms for the Tropical Rainfall Measuring Mission to retrieve rain and rain vertical structure from passive microwave radiometry. The self-consistency approach for surface rain rate retrieval of Kummerow et al. (1989) is applied to microwave brightness temperature data from the Cooperative Huntsville Meteorological Experiment (Dodge et al., 1986). Because the algorithm simultaneously retrieves rainfall rate and rain system structure, it is suggested that the algorithm can be used to overcome the problem of the dependence of brightness temperatures on the vertical profile of hydrometeors.

Kummerow, Christian↗

Aircraft microwave observations and simulations of deep convection from 18 to 183 GHz. I - Observations

Aircraft passive microwave observations of deep atmospheric convection at frequencies between 18 and 183 GHz are presented in conjunction with visible and infrared satellite and aircraft observations and ground-based radar observations. Deep convective cores are indicated in the microwave data by negative brightness temperature, T/(B) deviations from the land background (270 K) to extreme T(B) values below 100 K at 37, 92, and 183 GHz and below 200 K at 18 GHz. These T(B) minima, due to scattering by ice held aloft by the intense updrafts, are well correlated with areas of high radar reflectivity. For this land background case, T(B) is inversely correlated with rain rate at all frequencies due to T(B)-ice-rain correlations. Mean Delta-T between vertically polarized and horizontally polarized radiance in precipitation areas is approximately 6 K at both 18 GHz and 37 GHz, indicating nonspherical precipitation-size ice particles with a preferred horizontal orientation. Convective cores not observed in the visible and infrared data are clearly defined in the microwave observations, and borders of convective rain areas are well defined using the high-frequency (90 GHz and greater) microwave observations.

Adler, Robert F.↗

Solving time-dependent two-dimensional eddy current problems

Transient eddy current calculations are presented for an EM wave-scattering and field-penetrating case in which a two-dimensional transverse magnetic field is incident on a good (i.e., not perfect) and infinitely long conductor. The problem thus posed is of initial boundary-value interface type, where the boundary of the conductor constitutes the interface. A potential function is used for time-domain modeling of the situation, and finite difference-time domain techniques are used to march the potential function explicitly in time. Attention is given to the case of LF radiation conditions.

Lee, Min Eig↗

Detection of reflector surface from near field phase measurements

The deviation of a reflector antenna surface from a perfect parabolic shape causes degradation of the performance of the antenna. The problem of determining the shape of the reflector surface in a reflector antenna using near field phase measurements is not a new one. A recent issue of the IEEE tansactions on Antennas and Propagation (June 1988) contained numerous descriptions of the use of these measurements: holographic reconstruction or inverse Fourier transform. Holographic reconstruction makes use of measurement of the far field of the reflector and then applies the Fourier transform relationship between the far field and the current distribution on the reflector surface. Inverse Fourier transformation uses the phase measurements to determine the far field pattern using the method of Kerns. After the far field pattern is established, an inverse Fourier transform is used to determine the phases in a plane between the reflector surface and the plane in which the near field measurements were taken. These calculations are time consuming since they involve a relatively large number of operations. A much faster method can be used to determine the position of the reflector. This method makes use of simple geometric optics to determine the path length of the ray from the feed to the reflector and from the reflector to the measurement point. For small physical objects and low frequencies, diffraction effects have a major effect on the error, and the algorithm provides incorrect results. It is believed that the effect is less noticeable for large distortions such as antenna warping, and more noticeable for small, localized distortions such as bumps and depressions such as might be caused by impact damage.

Ida, Nathan↗

Determination of precipitation profiles from airborne passive microwave radiometric measurements

This study presents the first quantitative retrievals of vertical profiles of precipitation derived from multispectral passive microwave radiometry. Measurements of microwave brightness temperature (Tb) obtained by a NASA high-altitude research aircraft are related to profiles of rainfall rate through a multichannel piecewise-linear statistical regression procedure. Statistics for Tb are obtained from a set of cloud radiative models representing a wide variety of convective, stratiform, and anvil structures. The retrieval scheme itself determines which cloud model best fits the observed meteorological conditions. Retrieved rainfall rate profiles are converted to equivalent radar reflectivity for comparison with observed reflectivities from a ground-based research radar. Results for two case studies, a stratiform rain situation and an intense convective thunderstorm, show that the radiometrically derived profiles capture the major features of the observed vertical structure of hydrometer density.

Kummerow, Christian↗

Aircraft multifrequency passive microwave observations of light precipitation over the ocean

Aircraft passive microwave observations at 18, 37, 92, and 183 GHz of light oceanic precipitation are studied in conjunction with visible and infrared observations and ground-based radar data. Microwave signatures for clear, cloudy, and precipitating conditions are defined, with results in general agreement with previous theoretical results. Emission signatures are evident at 18, 37, and 92 GHz with clouds and precipitation producing an increase in brightness temperature over that observed over the low-emissivity ocean background. Polarization differences at 18 and 37 GHz also decrease in precipitation areas to minima of 30 K at 18 GHz and 15 K at 37 GHz. The 92-GHz brightness temperature shows a double-valued relationship, with an increase in cloudy and very lightly raining areas and a subsequent decrease for higher rain rates and deeper clouds where the ice scattering process becomes important. The 183-GHz observations display a distinct sensitivity to small amounts of ice. Simple channel differences are shown to compare favorably to the rain field, including polarization differences at 18 and 37 GHz and frequency differences between 92 and 37 GHz and between 183 and 92 GHz.

Adler, Robert F.↗

Determination of maritime snowfall from radar and microwave radiometer measurements

This study examines the effect of snowfall on high frequency microwave radiances measured from above the atmosphere by airborne radiometers. Attention is given to the analysis of a maritime snow storm so that snow accumulation on the surface would not introduce ambiguities into the analysis of the upwelling radiances.

Weinman, James A.↗

Monitoring and controlling distributed applications using Lomita (position paper)

Over the last four years, the Meta toolkit was developed for controlling distributed applications. This toolkit has been publicly available as part of the academic ISIS release, and has been used for building various system monitoring and control applications. One major stumbling block with using Meta has been the language (called NPL) it supports. NPL is very low-level and using it is difficult, in the same way it is difficult to write machine language programs or raw Postscript programs. Hence, a higher level language was built along with a runtime environment. The hope is that with this higher-level approach, more complicated Meta applications will be written and thereby concentrate more on the use (and limitations) of Meta as an architecture. The Meta toolkit is reviewed with its intended use. Next, the goals with Lomita and an overview is given of its architecture and language syntax. A detailed example is given of Lomita's use by presenting a complete program for a load-adaptable service.

Marzullo, Keith↗

Estimation of monthly rainfall over Japan and surrounding waters from a combination of low-orbit microwave and geosynchronous IR data

A method is described for estimating the mean monthly rainfall data for climate studies by combining the geosynchronous IR and low-orbit microwave data. The microwave technique uses the brightness temperature at 37 and 86 GHz from the Special Sensor Microwave/Imager instrument on board the DMSP satellite to define raining areas over water and land, and the 86-GHz scattering signal to assign rain rate based on cloud model-microwave calculations. The IR estimates are initially computed separately, using hourly data from the Japanese Geostationary Meteorological Satellite. Results show that, in areas where the microwave technique performs well, the combined microwave-IR monthly total estimates have better error statistics than either the microwave of the IR techniques individually.

Adler, Robert F.↗