Phenotypic Expression and Stability in a Large-Scale Field Study of Genetically Engineered Poplars Containing Sexual Containment Transgenes
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Candidate phylum OD1 bacteria (also referred to as Parcubacteria) have been identified in broad range of anoxic environments through community survey analysis. Although none of these species have been isolated in the laboratory, several genome sequences have been reconstructed from metagenomic sequence data and single-cell sequencing. The organisms have small (generally <1 Mb) genomes with severely reduced metabolic capabilities. We have reconstructed 8 partial to near-complete OD1 genomes from oxic groundwater samples, and compared them against existing genomic data. The conserved core gene set comprises 202 genes, or ~28% of the genomic complement. ‘Housekeeping’ genes and genes for biosynthesis of peptidoglycan and Type IV pilus production are conserved. Gene sets for biosynthesis of cofactors, amino acids, nucleotides and fatty acids are absent entirely or greatly reduced. The only aspects of energy metabolism conserved are the non-oxidative branch of the pentose-phosphate shunt and central glycolysis. These organisms also lack some activities conserved in almost all other known bacterial genomes, including signal recognition particle, pseudouridine synthase A, and FAD synthase. Pan-genome analysis indicates a broad genotypic diversity and perhaps a highly fluid gene complement, indicating historical adaptation to a wide range of growth environments and a high degree of specialization. The genomes were examined for signatures suggesting either a free-living, streamlined lifestyle or a symbiotic lifestyle. The lack of biosynthetic capabilities and DNA repair, along with the presence of potential attachment and adhesion proteins suggest the Parcubacteria are ectosymbionts or parasites of other organisms. The wide diversity of genes that potentially mediate cell-cell contact suggests a broad range of partner/prey organisms across the phylum.
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Liquid undergoing nonlinear Marangoni instability in a circular cylinder is examined, with attention given to roll-cell development and interaction. Surface deflections are neglected and the side walls are considered as adiabatic and impenetrable, allowing the liquid to freely slip. The nonlinear convective states are calculated and their stability is defined. The behavior and amplitude of cells forming in the liquid, heated from below, are modeled in order to derive all the transport properties. A new small parameter is formulated which is related to the critical Marangoni number of the infinite matrix expressing the eigenvalue expansion of the problem. The observed roll cell amplitudes and transport properties are shown to be available from simple eigenvalues, with double eigenvalues, indicating the existence of two roll-states as predicted by linear theory, nonlinear theory indicates transitions from one steady convective state to another.
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This NEUP funded project, NEUP 12-3630, is for experimental, numerical and analytical studies on high-pressure steam condensation phenomena in a steel containment vessel connected to a water cooling tank, carried out at Oregon State University (OrSU) and the University of Wisconsin at Madison (UW-Madison). In the three years of investigation duration, following the original proposal, the planned tasks have been completed: (1) Performed a scaling study for the full pressure test facility applicable to the reference design for the condensation heat transfer process during design basis accidents (DBAs), modified the existing test facility to route the steady-state secondary steam flow into the high pressure containment for controllable condensation tests, and extended the operations at negative gage pressure conditions (OrSU). (2) Conducted a series of DBA and quasi-steady experiments using the full pressure test facility to provide a reliable high pressure condensation database (OrSU). (3) Analyzed experimental data and evaluated condensation model for the experimental conditions, and predicted the prototypic containment performance under accidental conditions (UW-Madison). A film flow model was developed for the scaling analysis, and the results suggest that the 1/3 scaled test facility covers large portion of laminar film flow, leading to a lower average heat transfer coefficient comparing to the prototypic value. Although it is conservative in reactor safety analysis, the significant reduction of heat transfer coefficient (50%) could under estimate the prototypic condensation heat transfer rate, resulting in inaccurate prediction of the decay heat removal capability. Further investigation is thus needed to quantify the scaling distortion for safety analysis code validation. Experimental investigations were performed in the existing MASLWR test facility at OrST with minor modifications. A total of 13 containment condensation tests were conducted for pressure ranging from 4 to 21 bar with three different static inventories of non-condensable gas. Condensation and heat transfer rates were evaluated employing several methods, notably from measured temperature gradients in the HTP as well as measured condensate formation rates. A detailed mass and energy accounting was used to assess the various measurement methods and to support simplifying assumptions required for the analysis. Condensation heat fluxes and heat transfer coefficients are calculated and presented as a function of pressure to satisfy the objectives of this investigation. The major conclusions for those tests are summarized below: (1) In the steam blow-down tests, the initial condensation heat transfer process involves the heating-up of the containment heat transfer plate. An inverse heat conduction model was developed to capture the rapid transient transfer characteristics, and the analysis method is applicable to SMR safety analysis. (2) The average condensation heat transfer coefficients for different pressure conditions and non-condensable gas mass fractions were obtained from the integral test facility, through the measurements of the heat conduction rate across the containment heat transfer plate, and from the water condensation rates measurement based on the total energy balance equation. 15 (3) The test results using the measured HTP wall temperatures are considerably lower than popular condensation models would predict mainly due to the side wall conduction effects in the existing MASLWR integral test facility. The data revealed the detailed heat transfer characteristics of the model containment, important to the SMR safety analysis and the validation of associated evaluation model. However this approach, unlike separate effect tests, cannot isolate the condensation heat transfer coefficient over the containment wall, and therefore is not suitable for the assessment of the condensation heat transfer coefficient against system pressure and noncondensable gas mass fraction. (4) The average condensation heat transfer coefficients measured from the water condensation rates through energy balance analysis are appropriate, however, with considerable uncertainties due to the heat loss and temperature distribution on the containment wall. With the consideration of the side wall conduction effects, the results indicate that the measured heat transfer coefficients in the tests is about 20% lower than the prediction of Dehbi’s correlation, mainly due to the side wall conduction effects. The investigation also indicates an increase in the condensation heat transfer coefficient at high containment pressure conditions, but the uncertainties invoked with this method appear to be substantial. (5) Non-condensable gas in the tests has little effects on the condensation heat transfer at high elevation measurement ports. It does affect the bottom measurements near the water level position. The results suggest that the heavier non-condensable gas is accumulated in the lower portion of the containment due to stratification in the narrow containment space. The overall effects of the non-condensable gas on the heat transfer process should thus be negligible for tall containments of narrow condensation spaces in most SMR designs. Therefore, the previous correlations with noncondensable gas effects are not appropriate to those small SMR containments due to the very poor mixing of steam and non-condensable gas. The MELCOR simulation results agree with the experimental data reasonably well. However, it is observed that the MELCOR overpredicts the heat flux for all analyzed tests. The MELCOR predicts that the heat fluxes for CCT’s approximately range from 30 to 45 kW/m2 whereas the experimental data (averaged) ranges from about 25 to 40 kW/m2. This may be due to the limited availability of liquid film models included in MELCOR. Also, it is believed that due to complex test geometry, measured temperature gradients across the heat transfer plate may have been underestimated and thus the heat flux had been underestimated. The MELCOR model predicts a film thickness on the order of 100 microns, which agrees very well with film flow model developed in this study for scaling analysis. However, the expected differences in film thicknesses for near vacuum and near atmospheric test conditions are not significant. Further study on the behavior of condensate film is expected to refine the simulation results. Possible refinements include but are not limited to, the followings: CFD simulation focusing on the liquid film behavior and benchmarking with experimental analyses for simpler geometries. 16 1 INTRODUCTION This NEUP funded project, NEUP 12-3630, is for experimental, numerical and analytical studies on high-pressure steam condensation phenomena in a steel containment vessel connected to a water cooling tank, carried out at Oregon State University (OrSU) and the University of Wisconsin at Madison (UW-Madison). The experimental results are employed to validate the containment condensation model in reactor containment system safety analysis code for integral SMRs. Such a containment condensation model is important to demonstrate the adequate cooling. In the three years of investigation, following the original proposal, the following planned tasks have been completed: (1) Performed a scaling study for the full pressure test facility applicable to the reference design for the condensation heat transfer process during design basis accidents (DBAs), modified the existing test facility to route the steady-state secondary steam flow into the high pressure containment for controllable condensation tests, and extended the operations at negative gage pressure conditions (OrSU). (2) Conducted a series of DBA and quasi-steady experiments using the full pressure test facility to provide a reliable high pressure condensation database (OrSU). (3) Analyzed experimental data and evaluated condensation model for the experimental conditions, and predicted the prototypic containment performance under accidental conditions (UW-Madison). The results are applicable to integral Small Modular Reactor (SMR) designs, including NuScale, mPower, Westinghouse SMR, Holtec-160 and other integral reactors with small containments of relatively high pressures under accidental conditions. Testing has been conducted at the OrSU laboratory in the existing MASLWR (Multi-Application Small Light Water Reactor) integral test facility sponsored by the US Department of Energy. Its highpressure stainless steel containment model (~2 MPa) is scaled to the NuScale SMR currently under development at NuScale Power, Inc.. Minor modifications to the model containment have been made to control the non-condensable gas fraction and to utilize the secondary loop stable steam flow for condensation testing. UW-Madison has developed a containment condensation model, which leveraged previous validated containment heat transfer work carried out at UW-Madison, and extended the range of applicability of the model to integral SMR designs that utilize containment vessels of high heat transfer efficiencies. In this final report, the research background and literature survey are presented in Chapter 2 and 3, respectively. The test facility description and modifications are summarized in Chapter 4, and the scaling analysis is introduced in Chapter 5. The tests description, procedures, and data analysis are presented in Chapter 6, while the numerical modeling is presented in Chapter 7, followed by a conclusion section in Chapter 8.
Radioactive Waste Management Limited (RWM), a fully owned subsidiary of the Nuclear Decommissioning Authority (NDA), is responsible for the implementation of geological disposal in the UK. Three illustrative concepts of a geological disposal facility (GDF), corresponding to three generic geological environments - higher strength rock, lower strength sedimentary rock and evaporite rock - have been developed to demonstrate the viability of geological disposal of intermediate level waste, high level waste and spent fuel. It is envisaged that HLW which has been encapsulated in Waste Vitrification Plant (WVP) canisters, advanced gas-cooled reactor (AGR) SF which has been stripped of their spacers and packaged into slotted fuel cans, and PWR SF will be packaged into disposal containers for disposal. A set of schematic designs of these disposal containers was developed in the late 2000's. The design concept for the higher strength rock environment was based on the joint Posiva/SKB disposal container design, and the design concept for the lower strength sedimentary rock and evaporate rock illustrative environments was based on the disposal container concept from Switzerland's National Cooperative for the Disposal of Radioactive Waste (Nagra). The size, mass and dimensions of the disposal containers in the scheme designs vary significantly. The construction method and the lid closing method also vary between the disposal containers for the different waste contents. A design study has indicated that optimization of the number of HLW WVP canisters and the number of AGR slotted fuel cans in the disposal containers, standardizing construction method and closure arrangement across the containers for each geology type, providing handling features at both ends of the disposal container, and balancing of shielding around the containers, could bring significant advantage to the overall GDF programme by reducing the number of disposal containers, reducing GDF footprint, and reducing the extent of excavations, spoil generated and materials that are required for construction. A project to develop the scheme designs to conceptual level was carried in 2011 to 2013. In this project, the above changes were taken into account, technical underpinning of the designs was carried out and manufacturing method was considered. Two disposal container variants for different host rock environments have been developed: - Variant 1, for compatibility with the higher strength rock illustrative disposal system design. This is constructed from copper, with a cast iron insert and is based on the joint Posiva/SKB disposal container design. - Variant 2, for compatibility with the lower strength sedimentary rock and evaporite rock illustrative disposal system designs. This is a steel container based on the NAGRA disposal container design. Each variant has three internal configurations to accommodate 3 HLW vitrified canisters, 16 consolidated cans of AGR SF and 4 PWR spent fuel assemblies. These designs have been shown to bring significant benefit to the GDF designs, bringing a significant reduction to the operating programme and GDF programme cost, including reducing the number of disposal containers by 33% for HLW, 50% for AGR SF and reducing the size of the GDF footprint by 25%. (authors)
Destructive evaluation (DE) of 3013 containers is one part of the U. S. Department of Energy Integrated Surveillance Program. During standard DE of 3013 containers, visual examinations for pitting and stress corrosion cracking (SCC) are performed on the accessible surfaces of the outer, inner, and convenience containers, which make up the 3013 container. As a result of 3013 DE additional analysis, the area near the inner container closure weld has been identified as being a region of increased corrosion susceptibility, which may provide a pathway for corrosive gases to the outer container. This area has a higher residual stress, an altered microstructure, and less corrosion resistant weld oxides as a result of the welding process as well as a lower temperature than other areas of the container, which may increase the absorption of moisture on the surface. The deposition of moisture in this stressed region could lead to pitting and stress corrosion cracking. During FY2013, the inner container closure weld area was more closely evaluated on several archived samples from DE containers. These containers included FY09 DE2, FY12 DE4, FY12 DE6 and FY12 DE7 and the Hanford High Moisture Container. The additional examinations included visual observations with a stereomicroscope, scanning electron microscopy along with energy dispersive spectroscopy for chemical analysis, and serial metallography of the sidewall and lid that are part of the inner container closure weld region. Pitting was observed in all the samples taken from the closure weld regions of the examined inner containers. This pitting was generally less 20 µm with most less than 5µm. These pits were similar in depth to those observed in the vapor exposed surfaces of teardrops in the shelf life corrosion testing. Cracking was not observed on either the vapor-exposed surfaces of the teardrop coupons or the inner container closure weld region. Further testing is necessary to determine if the conditions in the welded inner container could support SCC during the 50 year life time for the 3013 container.
Japanese spacecraft Hayabusa, which returned from near-Earth-asteroid Itokawa, successfully returned its reentry capsule to the Earth, the Woomera Prohibited Area in Australia in Jun 13th, 2010, as detailed in another paper [1]. The capsule introduced into the Planetary Material Sample Curation Facility in the Sagamihara campus of JAXA in the early morning of June 18th. Hereafter, we describe a series of processes for the returned capsule and the container to recover gas and materials in there. A transportation box of the recovered capsule was cleaned up on its outer surface beforehand and introduced into the class 10,000 clean room of the facility. Then, the capsule was extracted from the box and its plastic bag was opened and checked and photographed the outer surface of the capsule. The capsule was composed of the container, a backside ablator, a side ablator, an electronic box and a supporting frame. The container consists of an outer lid, an inner lid, a frame for latches, a container and a sample catcher, which is composed of room A and B and a rotational cylinder. After the first check, the capsule was packed in a plastic bag with N2 again, and transferred to the Chofu campus in JAXA, where the X-ray CT instrument is situated. The first X-ray CT analysis was performed on the whole returned capsule for confirming the conditions of latches and O-ring seal of the container. The analysis showed that the latches of the container should have worked normally, and that the double Orings of the container seemed to be sealed its sample catcher with no problem. After the first X-ray CT, the capsule was sent back to Sagamihara and introduced in the clean room to exclude the electronic box and the side ablator from the container by hand tools. Then the container with the backside ablator was set firmly to special jigs to fix the lid of container tightly to the container and set to a milling machine. The backside ablator was drilled by the machine to expose heads of bolts, which combined the ablator to the outer lid of the container, and after the drilling had been finished, all the bolts were unscrewed and the backside ablator was removed from the container. Then, the container was sent to the Chofu X-ray facility again to examine in detail by a micro X-ray CT instrument in order to reconfirm that the condition of the latches of the lid of container was normal and that its double O-ring seemed to have been sealed after the last X-ray CT analysis.
As well as working in the field of waste management, Kerntechnische Entsorgung Karlsruhe GmbH (KBE) is, for historical reasons, one of Germany's three constructors of repository containers for the repository Konrad. The focus of this container production is merely on the side of requalification of the existing legacy containers, which are partly already loaded and grouted. To a smaller extend, KTE also produces new containers for its own use because the containers exactly match the waste properties present on the Karlsruhe site. There are three major container types in the process of requalification: - The Type-IV-Drum-Steel-Container FSC-IV-D was successfully licensed in 2014 and regularly produced thereafter. At the same time, the pre-existing containers of this type are re-qualified on the basis of this license by proof of the compliance with this license. - The Type-IV-Product-Container PSC-I-D is identical to the FSC-VI-D in terms of steel construction but also contains a liner of concrete for shielding and protection of the steel construction. This container is designed for large components and demolition waste. After a successful drop test, it is currently in the advanced process of licensing. - Type I cylindrical concrete containers are simple single drum shieldings which were produced in large numbers in the 1970's and 1980's. KTE owns 7,000 of those containers, thus making requalification and licensing cheaper than retreatment of the waste after demolition of the containers. (authors)
The SAVY 4000 container was designed by Nuclear Filter Technology, Inc. to meet DOE M441.1-1 requirements for safe storage of nuclear material, and it is currently being used at Los Alamos National Laboratory, Livermore National Laboratory, Idaho National Laboratory and Nevada National Security Site. The 316L stainless steel container can be manufactured in a range of sizes from 1 quart to 10 gallon capacity, it can be opened and closed easily by hand, it is sealed with a Viton{sup R} O-ring, and the filter retains particulates and resists liquid water entry while allowing hydrogen gas to escape. This innovative design has proven to provide benefits and uses beyond its original intent. For example, the container was tested in a simulated facility design basis accident involving high temperatures, fuel fire, and subsequent drop, and the damage ratio was measured. This has allowed the LANL plutonium facility to take credit for a material-at-risk reduction of approximately two orders of magnitude for material stored in the SAVY 4000 container. In addition, the water resistant filter allows the nuclear facility to credit the container as a safety feature to mitigate the risk of a criticality accident in the event of facility sprinkler system activation. The container has also been approved to transfer small quantities of solid nuclear material by hand carry between Los Alamos facilities, and it has been approved and used as a waste container for transuranic waste destined for the Waste Isolation Pilot Plant. The 1 quart capacity container and the future 2 quart design both fit inside the 9977 shipping container, and this has facilitated the transport of nuclear material from Idaho National Laboratory to Nevada Nuclear Security Site, obviated the need for repackaging the material for storage upon arrival. Finally, Los Alamos has recently contracted with Nuclear Filter Technology, Inc. to perform testing to certify the SAVY 4000 container as a DOT Type A liquid transport container. This paper will provide an overview of the various testing regimes and describe the resulting versatility of the container for nuclear material containment. (authors)