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At least 217 records · Page 12
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Single-nuclei transcriptome analysis of IgM+ cells isolated from channel catfish (Ictalurus punctatus) spleen

Catfish production is the primary aquaculture sector in the United States, and the key cultured species is channel catfish (Ictalurus punctatus). The major causes of production losses are pathogenic diseases, and the spleen, an important site of adaptive immunity, is implicated in these diseases. To examine the channel catfish immune system, single-nuclei transcriptomes of sorted and captured IgM + cells were produced from adult channel catfish. Three channel catfish (~1 kg) were euthanized, the spleen dissected, and the tissue dissociated. The lymphocytes were isolated using a Ficoll gradient and IgM + cells were then sorted with flow cytometry. The IgM + cells were lysed and single-nuclei libraries generated using a Chromium Next GEM Single Cell 3’ GEM Kit and the Chromium X Instrument (10x Genomics) and sequenced with the Illumina NovaSeq X Plus sequencer. The reads were aligned to theI. punctatusreference assembly (Coco_2.0) using Cell Ranger, and normalization, cluster analysis, and differential gene expression analysis were carried out with Seurat. Across the three samples, approximately 753.5 million reads were generated for 18,686 cells. After filtering, 10,637 cells remained for the cluster analysis. The cluster analysis identified 16 clusters which were classified as B cells (10,276), natural killer-like (NK-like) cells (178), T cells or natural killer cells (45), hematopoietic stem and progenitor cells (HSPC)/megakaryocytes (MK) (66), myeloid/epithelial cells (40), and plasma cells (32). The B cell clusters were further defined as different populations of mature B cells, cycling B cells, and plasma cells. The plasma cells highly expressedighmand we demonstrated that the secreted form of the transcript was largely being expressed by these cells. This atlas provides insight into the gene expression of IgM + immune cells in channel catfish. The atlas is publicly available and could be used garner more important information regarding the gene expression of splenic immune cells.

Immunology↗
DOE OSTI2023

BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event

Determining which microorganisms are active within soil communities remains a major technical endeavor in microbial ecology research. One promising method to accomplish this is coupling bioorthogonal non-canonical amino acid tagging (BONCAT) with fluorescence activated cell sorting (FACS) which sorts cells based on whether or not they are producing new proteins. Combined with shotgun metagenomic sequencing (Seq), we apply this method to profile the diversity and potential functional capabilities of both active and inactive microorganisms in a biocrust community after being resuscitated by a simulated rain event. We find that BONCAT-FACS-Seq is capable of discerning the pools of active and inactive microorganisms, especially within hours of applying the BONCAT probe. The active and inactive components of the biocrust community differed in species richness and composition at both 4 and 21 h after the wetting event. The active fraction of the biocrust community is marked by taxa commonly observed in other biocrust communities, many of which play important roles in species interactions and nutrient transformations. Among these, 11 families within the Firmicutes are enriched in the active fraction, supporting previous reports indicating that the Firmicutes are key early responders to biocrust wetting. We highlight the apparent inactivity of many Actinobacteria and Proteobacteria through 21 h after wetting, and note that members of the Chitinophagaceae, enriched in the active fraction, may play important ecological roles following wetting. Based on the enrichment of COGs in the active fraction, predation by phage and other bacterial members, as well as scavenging and recycling of labile nutrients, appear to be important ecological processes soon after wetting. To our knowledge, this is the first time BONCAT-FACS-Seq has been applied to biocrust samples, and therefore we discuss the potential advantages and shortcomings of coupling metagenomics to BONCAT to intact soil communities such as biocrust. In all, by pairing BONCAT-FACS and metagenomics, we are capable of highlighting the taxa and potential functions that typifies the microbes actively responding to a rain event.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Design of solvent-assisted plastics recycling: Integrated economics and environmental impacts analysis

In 2018, the United States generated over 35. 7 million tons of plastic waste, with only 8.4% being recycled and the other 91.6% incinerated or disposed of in a landfill. The continued growth of the polymer market has raised concerns over the end of life of plastics. Currently, the waste management system is faced with issues of inefficient sorting methods and low-efficiency recycling methods when it comes to plastics recycling. Mechanical recycling is the commonest recycling method but presents a lower-valued recycled material due to the material incompatibilities introduced via the inefficient sorting methods. Chemical recycling offers a promising alternative as it potentially allows for plastics to maintain their original properties. To that end, there is the need to investigate feasible chemical recycling methods to help mitigate the challenging problem posed by plastics at the end-of-life stage. This work proposes a conceptual solvent-assisted plastics recycling framework based on a superstructure optimization approach. This framework is evaluated using a representative case study to recover Polyethylene Terephthalate (PET). In this case study, it is found that polymer recovery is both economically and environmentally favorable when compared to traditional methods of disposal such as incineration.

Lehr, Austin L.↗
DOE OSTI2023

Improved Tomographic Binning of 3 × 2 pt Lens Samples: Neural Network Classifiers and Optimal Bin Assignments

Abstract Large imaging surveys, such as the Legacy Survey of Space and Time, rely on photometric redshifts and tomographic binning for 3 × 2 pt analyses that combine galaxy clustering and weak lensing. In this paper, we propose a method for optimizing the tomographic binning choice for the lens sample of galaxies. We divide the CosmoDC2 and Buzzard simulated galaxy catalogs into a training set and an application set, where the training set is nonrepresentative in a realistic way, and then estimate photometric redshifts for the application sets. The galaxies are sorted into redshift bins covering equal intervals of redshift or comoving distance, or with an equal number of galaxies in each bin, and we consider a generalized extension of these approaches. We find that bins of equal comoving distance produce the highest dark energy figure of merit of the initial binning choices, but that the choice of bin edges can be further optimized. We then train a neural network classifier to identify galaxies that are either highly likely to have accurate photometric redshift estimates or highly likely to be sorted into the correct redshift bin. The neural network classifier is used to remove poor redshift estimates from the sample, and the results are compared to the case when none of the sample is removed. We find that the neural network classifiers are able to improve the figure of merit by ∼13% and are able to recover ∼25% of the loss in the figure of merit that occurs when a nonrepresentative training sample is used.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

Projected Urban Morphology of the Los Angeles Area by the Year 2100

This dataset provides projections of urban building morphologies for the Los Angeles urban area at 30-meter spatial resolution. It contains 192 raster files that detail two primary building attributes: building footprint fractions (ranging from 0 to 1) and average building heights (ranging from 0 to 75 meters). The projections account for a wide range of future pathways, covering two Shared Socioeconomic Pathway (SSP) scenarios (SSP3 and SSP5), two population scenarios, two developed land intensification scenarios, and four distinct levels of intensification. The dataset was created using dual Generative Adversarial Networks (GANs) trained on 2015 land cover and building properties from the National Land Cover Database (NLCD) and Model America datasets. Supporting information on the dataset has been described in the LAUrbanAreaMorphologyProjections2100_README.txt file.

Pandey, Bhartendu↗
NASA NTRS1973

An automated procedure for computing flutter eigenvalues.

A new, fast and economical automated procedure for implementing the traditional V-g method of flutter solution is described. The procedure requires as input the generalized aerodynamic forces for a range of reduced frequencies obtained from an aerodynamic program. These aerodynamic forces are interpolated with respect to reduced frequency using a newly developed, partially tabulated cubic spline that is both fast in execution and economical in storage. The flutter solution is then obtained using an eigenvalue routine that has been developed to take advantage of the parametric nature of the V-g type of solution. Furthermore, the routine takes care of the fundamental and troublesome problem of properly sorting the output eigenvalues. By solving the root-sorting problem, the interpolation for flutter crossings and automatic plotting are accomplished efficiently. The computational techniques used in this new program are described and some sample results are given.

Desmarais, R. N.↗
NASA NTRS1978

Data Management Symposium, Huntsville, Ala., October 18, 19, 1977, Proceedings

The transfer, processing, and use of satellite data are discussed. Topics relating to simulation and processing techniques include computer design assessments through simulation, data system dynamic simulation, and future timing accuracy requirements and procedures for data processing. Subjects relevant to data base management systems and users and their needs include an image-based information system architecture for correlation satellite and topological data base, efficient searching and sorting applications using an associative array processor, analysis of user's needs for a large technical data base, and variable length data formats. The technology development outlook was considered with attention to data systems technology outlook for supporting NASA programs, a user-oriented interactive information extraction system, and flow control and sorting techniques for telemetry packets.

Castelli, A.↗
NASA NTRS1978

HD-SAO-DM cross index

A table of correspondence SAO-HD-DM-GC was prepared by Morin (1973). The machine-readable version of this cross identification was obtained from the Centre de Donnees Stellaires (Strasbourg, France). The table was sorted at the Goddard Space Flight Center by HD number and all blank HD number records were removed to produce the HD-SAO-DM table presented. There were 258997 entries in the original table; there are 180411 entries after removing the blank HD records. The Boss General Catalogue (GC) numbers were retained on the machine-readable version after the sort.

Nagy, T. A.↗
NASA NTRS1981

Temporal and spatial variations observed in the ionospheric composition of Venus - Implication for empirical modelling

The distributions of several ion species in a data base obtained by in situ measurements of the thermal ion composition of the ionosphere of Venus by the Pioneer Venus Orbiter have been sorted to identify temporal and spatial variations and determine the feasibility of an analytical representation of the experimental results. The first results from the sorting of several prominent ions including O(+), O2(+), and H(+) and several minor ions including CO2(+), C(+), and H2(+) reveal significant diurnal variations which consist of strong day to night contrast in the ion concentrations, with differences of one to two orders of magnitude, depending upon ion mass and altitude. It is suggested that repeatable day to night gradients in the ion distribution are adaptable to parametric modeling.

Taylor, H. A., Jr.↗
NASA NTRS1982

Documentation for the machine-readable AGK3-BD and BD-AGK3 cross-index catalogues

The machine-readable cross identification catalogs were prepared by extracting AGK3 and BD numbers from the magnetic tape version of the AGK3 Catalog. After preparation of the AGK3-BD file, which contains all stars in the AGK3 catalog, the file was sorted according to BD number and rearranged so that the BD number occurs at the beginning of each record. During the sorting operation, all AGK3 stars having no BD numbers were omitted. The BD-AGK3 file is especially useful for finding BD stars in the AGK3 catalog, since precession has moved stars across declination boundaries and it often was necessary to examine more than one AGK3 zone to locate a BD star in the catalog. The contents of the cross index files is described so that users can read and process the tape without problems, guesswork, or consulting the parent catalog.

Warren, W. H., Jr.↗
NASA NTRS1982

The JPL millimeter, submillimeter and microwave line catalog

The catalog includes molecules of interest in the interstellar medium, in planetary atmospheres and in the Earth's atmosphere. Different units than those on the AFGL tape are used. For citing line frequencies in the microwave region. Either the experimental errors or the propagation of errors from the fit are included on the tape. This gives an estimate of how accurate the frequencies are. The intensity units are defined as follows: the logarithm of the intensity unit is the cross section times the frequency in MHz. This is essentially the same unit as on the AFGL tape, but contains fewer factors of the speed of light. The catalog is available to the scientific community either as a tape or on microfiche (filmed in frequency sorted and molecule sorted format).

Pickett, H.↗
NASA NTRS1982

Petrology of dune sand derived from basalt on the Ka'u Desert, Hawaii

Dune sand from the Ka'u Desert, southwest flank of Kilauea volcano, Hawaii, is moderately well-sorted (median = 1.60 Phi, deviation = 0.60, skewness = 0.25, kurtosis = 0.68) and composed mostly of frosted subangular particles of basalt glass ('unfractionated' olivine-normative tholeitte), olivine, lithic fragments (subophitic and intersertal basalts; magnetite-ilmenite-rich basalts), reticular basalt glass, magnetite, ilmenite, and plagioclase, in approximately that order of abundance. Quantitative lithological comparison of the dune sand with sand-sized ash from the Keanakakoi Formation supports suggestions that the dune sand was derived largely from Keanakakoi ash. The dune sand is too well sorted to have been emplaced in its present form by base-surge but could have evolved by post-eruption reworking of the ash.

Gooding, J. L.↗
NASA NTRS1983

Thermionic energy converters

The efficiency of thermionic energy converters is improved by internal distribution of tiny sorted cesium diodes driven by the thermal gradient between the primary emitter and the collector. The tiny, sorted diode distribution comprises protrusions of the emitter material from the main emitter face which contact the main collector face thermally but not electrically. The main collector ends of the protrusions are separated from the main collector by a thin layer of insulation, such as aluminum oxide. The shorted tiny diode distribution augments cesium ionization through internal thermal effects only within the main diode. No electrical inputs are required. This ionization enhancement by the distribution of the tiny shorted diodes not only reduces the plasma voltage drop but also increases the power output and efficiency of the overall thermionic energy converter.

Morris, J. F.↗
NASA NTRS1984

Postprocessing for the UCLA climate model

Postprocessing programs written to analyze the results of a general circulation model are described. The model history data is interpolated to pressure surfaces, converted from 64-bit Cyber words to 32 bit IBM words, and transferred to tape for further analysis. The time average program reads the interpolation output tape and produces 15-day time averages. The model outputs a very detailed record for selected grid points. This data is sent to a tape in Cyber full words. The conversion program changes the format of the tape from 64 bit Cyber words to 32 bit IBM words, processing a month of data at a time. The grid-point history sorting program inputs the converted grid point history tape and sorts it by time. The end product is a tape with a time history of each field for every point. After the PBP data has been stored, it can be plotted. The plotting package is flexible, and allows the choice of fields and grid points. The one-dimensional fields are plotted as functions of time, and a daily summary is also produced. For the two-dimensional fields, time-height cross sections and daily summaries are produced. The model plotting package produces plots for all fields on the time average and pressure tapes. These plots include world maps, polar projections, zonal means, vectors, streamlines, and latitude-height plots.

Abeles, J.↗
NASA NTRS1984

Variation of the auroral Birkeland and current pattern associated with the north-south component of the IMF

In this study, the data set acquired from the vector magnetometer on board MAGSAT during the period October 1979 to June 1980 is examined. Auroral zone crossings were sorted by hourly averaged values of the Interplanetary Magnetic Field. The result is a catalog of sorted data files, from which color-coded magnetic local time invariant latitude plots have been generated that image the auroral Birkeland current pattern, over each prescribed parameter range. These images are used to statistically examine the dynamics of the current pattern. Results indicate that during the periods of B(z) less than 0, the region 1 and 2 current system expands toward lower latitudes accompanied by an expansion of the auroral zones. When B(z) is greater than 0, the region 1 and 2 currents continue to flow with greatly reduced amplitude in the presence of extensive small scale structure.

Bythrow, P. F.↗
NASA NTRS1984

Size dependence of chondrule textural types

Chrondrule textural types were studied for size sorted chondrules from the ordinary chondrites Dhajala, Eston and Chainpur and the CM chondrite Murchison. Aliquot samples from size sorted Dhajala chondrules were studied for their oxygen isotopic composition and chondrules from Weston were studied for their precompaction irradiation records by nuclear track technique. Correlations between chondrule textural types and oxygen isotope or track data were identified. A distinct dependence of chondrule textural type on chondrule size was evident in the data for both Dhajala and Weston chondrules. No significant deviation was noticed in the abundance pattern of nonporphyritic chondrules within individual size fractions in the 200 to 800 micron size interval. Overabundance is found of nonporphyritic chondrules in the 100 to 200 micron size fraction of Murchison chondrules, the trend is not as distinct for Chainpur chondrules. Two hundred microns is suggested as the cutoff size below which radiative cooling is extremely efficient during the chondrule forming process. It is suggested that this offers a possibility for use of physical and chemical characteristics of small chondrules to constrain the temperature history during the chondrule formation process.

Goswami, J. N.↗
NASA NTRS1985

A New NASTRAN Capability for Data Reduction

A new module, MODB, for the data reduction of NASTRAN results is described. NASTRAN analysis results can be filtered and sorted for minimum/maximum values and the printed output resulting from large NASTRAN runs can be limited based on a number of available user options. The sorting is done on stresses, forces and vector quantities like displacements, velocity, and acceleration. The module can be accessed via DMAP alters to existing rigid formats, and has been used on a large number of statics and dynamics problems resulting in considerable savings in cost, time, and the amount of printing.

Gallo, M.↗
NASA NTRS1985

The primary cosmic ray mass composition at energies above 10(14) eV

It is shown in this paper that the experimental data on extensive air showers at the energy interval 10 to the 15th power - 10 to the 17th power eV seems to be described best if it is assumed that the Galactic cosmic rays are described by some sort of a two component picture. The first component is of a mixed composition similar to that at lower energies and the second is dominated by protons. Overall spectrum starts to be enriched in protons at energies about 10 to the 15th power eV bu the effective mass of the primaries remains constant up to energies around 10 to the 16th power eV. That results from the fact that composition gradually changes from multi-component to mixture of protons and heavies. That picture receives also some sort of support from recent observations of relatively high number of nergetic protons in JACEE and Concorde experiments.

Gawin, J.↗