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At least 217 records · Page 12

Design and Fabrication of the Superconducting Horizontal Bend Magnet for the Super High Momentum Spectrometer at Jefferson Lab

A collaboration exists between NSCL and JLab to design and build JLab's Super High Momentum Spectrometer (SHMS) horizontal bend magnet that allows the bending of the 12 GeV/c particles horizontally by 3° to allow SHMS to reach angles as low as 5.5°. Two full size coils have been wound and are cold tested for both magnetic and structural properties. Each coil is built from 90 layers of single-turn SSC outer conductor cable. An initial test coil with one third the turns was fabricated to demonstrate that the unique saddle shape with fully contoured ends could be wound with Rutherford superconducting cable. Learned lessons during the trial winding were integrated into the two complete full-scale coils that are now installed in the helium vessel. The fabrication of the iron yoke, cold mass, and thermal shield is complete, and assembly of the vacuum vessel is in progress. This paper presents the process and progress along with the modified magnet design to reduce the fringe field in the primary beam region and also includes the impact of the changes on coil forces and coil restraint system.

43 PARTICLE ACCELERATORS↗

The Value of Information From Horizontal Distributed Acoustic Sensing Compared to Multicomponent Geophones Via Machine Learning

Abstract Faults play an important role in recharging many geothermal reservoirs, and seismic information can image the locations of these faults. The value of information (VOI) metric is used to objectively quantify and compare the value of two types of seismic receiver data via a machine learning approach. The demonstrated VOI methodology is novel by including spatial models from seismic data and obtaining the information statistics from machine learning. Our two-dimensional numerical experiments compare images created from sparsely spaced (80 m), two-component geophone sampling to high spatial resolution (1 m), single-component DAS. We used a three-fold cross validation of a U-Net convolutional neural networks to achieve average classification statistics. The results suggest that when horizontal sources are utilized, geophones and DAS identify reflectors and non-reflectors at roughly the same rate. The average F1 score for horizontal DAS is 0.939 and 0.931 for geophones. For images created from a vertical source, DAS performed marginally better (F1 = 0.919) than geophones (F1 = 0.877). Our transferrable methodology can provide guidance on which acquisition scenarios can improve images of important structures in the subsurface and present an efficient method for obtaining reliability statistics from high-dimensional, spatial data.

Energy & Fuels↗

Horizontal transfer of chromosomal DNA mediated by an integrative and conjugative element generates frequent localized recombination in Novosphingobium aromaticivorans

Horizontal gene transfer is an important evolutionary process by which DNA is exchanged between cells that are physically co-located but not direct evolutionary descendants. Horizontal transfer of highly divergent DNA is relatively easy to detect and can produce major phenotypic changes, exemplified by the acquisition of antibiotic resistance determinants. However, transfer of high-identity DNA, for example, between strains of the same species, is likely to be more frequent, harder to detect, and highly impactful in aggregate. In this work, we demonstrate that soil isolates of the alphaproteobacterium Novosphingobium aromaticivorans can exchange chromosomal DNA, leading to multiple unselected recombination events spanning approximately 10% of the chromosome. Chromosomal recombination was directional and more efficient near an integrative and conjugative element (ICE), and required a relaxase found in the ICE. Recombination could not be observed in strains from closely related Novosphingobium species. In combination, these results suggest that ICE-mediated recombination can efficiently recombine DNA within N. aromaticivorans, increasing the adaptive potential of the species while also enforcing species boundaries through preferential intraspecific recombination.

Allemann, Marco [ORNL]↗

Horizontal Gene Transfer to a Defensive Symbiont with a Reduced Genome in a Multipartite Beetle Microbiome

Symbiotic mutualisms of bacteria and animals are ubiquitous in nature, running a continuum from facultative to obligate from the perspectives of both partners. The loss of functions required for living independently but not within a host gives rise to reduced genomes in many symbionts. Although the phenomenon of genome reduction can be explained by existing evolutionary models, the initiation of the process is not well understood. Here, we describe the microbiome associated with the eggs of the beetle Lagria villosa, consisting of multiple bacterial symbionts related to Burkholderia gladioli, including a reduced-genome symbiont thought to be the exclusive producer of the defensive compound lagriamide. We show that the putative lagriamide-producing symbiont is the only member of the microbiome undergoing genome reduction and that it has already lost the majority of its primary metabolism and DNA repair pathways. The key step preceding genome reduction in the symbiont was likely the horizontal acquisition of the putative lagriamide lga biosynthetic gene cluster. Unexpectedly, we uncovered evidence of additional horizontal transfers to the symbiont’s genome while genome reduction was occurring and despite a current lack of genes needed for homologous recombination. These gene gains may have given the genome-reduced symbiont a selective advantage in the microbiome, especially given the maintenance of the large lga gene cluster despite ongoing genome reduction.

59 BASIC BIOLOGICAL SCIENCES↗

Global Tilt Irradiance to Direct Normal and Diffuse Horizontal Irradiance

GTI-DIRINT is a separation model for deriving the direct normal irradiance (DNI) and the diffuse horizontal irradiance (DHI) from the global tilted irradiance (GTI). The model is based on the DIRINT model, but substitutes the use of the GTI in place of the global horizontal irradiance (GHI).

Source record↗

Influences of Environmental Relative Humidity and Horizontal Scale of Subcloud Ascent on Deep Convective Initiation

This study examines two factors impacting initiation of moist deep convection: free-tropospheric environmental relative humidity (φ E ) and horizontal scale of subcloud ascent (R sub ), the latter exerting a dominant control on cumulus cloud width. A simple theoretical model is used to formulate a “scale selection” hypothesis: that a minimum R sub is required for moist convection to go deep, and that this minimum scale decreases with increasing φ E . Specifically, the ratio of R 2 sub to saturation deficit (1 - φ E ) must exceed a certain threshold value that depends on cloud-layer environmental lapse rate. Idealized, large-eddy simulations of moist convection forced by horizontally varying surface fluxes show strong sensitivity of maximum cumulus height to both φ E and R sub consistent with the hypothesis. Increasing R sub by only 300–400 m can lead to a large increase (>5 km) in cloud height. A passive tracer analysis shows that the bulk fractional entrainment rate decreases rapidly with R sub but depends little on φ E . However, buoyancy dilution increases as either R sub or φ E decreases; buoyancy above the level of free convection is rapidly depleted in dry environments when R sub is small. While deep convective initiation occurs with an increase in relative humidity of the near environment from moistening by earlier convection, the importance of this moisture preconditioning is inconclusive as it is accompanied by an increase in R sub . Overall, it is concluded that small changes to R sub driven by external forcing or by convection itself could be a dominant regulator of deep convective initiation.

54 ENVIRONMENTAL SCIENCES↗

The Perchlorate Reduction Genomic Island: Mechanisms and Pathways of Evolution by Horizontal Gene Transfer

Background: Perchlorate is a widely distributed anion that is toxic to humans, but serves as a valuable electron acceptor for several lineages of bacteria. The ability to utilize perchlorate is conferred by a horizontally transferred piece of DNA called the perchlorate reduction genomic island (PRI). Methods: We compared genomes of perchlorate reducers using phylogenomics, SNP mapping, and differences in genomic architecture to interrogate the evolutionary history of perchlorate respiration. Results: Here we report on the PRI of 13 genomes of perchlorate-reducing bacteria from four different classes of Phylum Proteobacteria (the Alpha-, Beta-, Gamma- and Epsilonproteobacteria). Among the different phylogenetic classes, the island varies considerably in genetic content as well as in its putative mechanism and location of integration. However, the islands of the densely sampled genera Azospira and Magnetospirillum have striking nucleotide identity despite divergent genomes, implying horizontal transfer and positive selection within narrow phylogenetic taxa. We also assess the phylogenetic origin of accessory genes in the various incarnations of the island, which can be traced to chromosomal paralogs from phylogenetically similar organisms. Conclusion: These observations suggest a complex phylogenetic history where the island is rarely transferred at the class level but undergoes frequent and continuous transfer within narrow phylogenetic groups. This restricted transfer is seen directly by the independent integration of near-identical islands within a genus and indirectly due to the acquisition of lineage-specific accessory genes. The genomic reversibility of perchlorate reduction may present a unique equilibrium for a metabolism that confers a competitive advantage only in the presence of an electron acceptor, which although widely distributed, is generally present at low concentrations in nature.

59 BASIC BIOLOGICAL SCIENCES↗

PoroTomo Natural Laboratory Horizontal and Vertical Distributed Acoustic Sensing Data

This dataset includes links to the PoroTomo DAS data in both SEG-Y and hdf5 (via h5py and HSDS with h5pyd) formats with tutorial notebooks for use. Data are hosted on Amazon Web Services (AWS) Simple Storage Service (S3) through the Open Energy Data Initiative (OEDI). Also included are links to the documentation for the dataset, Jupyter Notebook tutorials for working with the data as it is stored in AWS S3, and links to data viewers in OEDI for the horizontal (DASH) and vertical (DASV) DAS datasets. Horizontal DAS (DASH) data collection began 3/8/16, paused, and then started again on 3/11/2016 and ended 3/26/2016 using zigzag trenched fiber optic cabels. Vertical DAS (DASV) data collection began 3/17/2016 and ended 3/28/16 using a fiber optic cable through the first 363 m of a vertical well. These are raw data files from the DAS deployment at (DASH) and below (DASV) the surface during testing at the PoroTomo Natural Laboratory at Brady Hot Spring in Nevada. SEG-Y and hdf5 files are stored in 30 second files organized into directories by day. The hdf5 files available via HSDS are stored in daily files. Metadata includes information on the timing of recording gaps and a file count is included that lists the number of files from each day of recording. These data are available for download without login credentials through the free and publicly accessible Open Energy Data Initiative (OEDI) data viewer which allows users to browse and download individual or groups of files.

15 GEOTHERMAL ENERGY↗

PoroTomo: Horizontal Distributed Acoustic Sensing (DAS) Measurements During an M 2.3 Explosion

Included here are Distributed Acoustic Sensing (DAS) data collected by the horizontal DAS array at Brady's Hot Springs Geothermal Field. The system recorded this data during an M 2.3 explosion at the Nevada Test Site (NTS), which is located approximately 400km southeast of the field. On December 19, 2018, a Silixa iDAS interrogator continuously acquired data on single-mode fibers, starting approximately two hours prior to the detonation and extending until 15 minutes after the event. This dataset comprises separate SEG-Y files that span the entire recording interval. The measurement fiber was part of the PoroTomo experiment and is horizontally emplaced in alluvial fan gravels at a depth of approximately one meter for a distance of more than 2.5 km in a zig-zag pattern. Exact coordinates of this DAS surface array are provided in the attached GDR dataset below. The iDAS interrogator channel spacing is 1.021 m and gauge length is 10 m. Concurrent Distributed Temperature Sensing (DTS) measurements made with a Silixa XT on the multi-mode fibers aided in calibrating the DAS measurements.

15 GEOTHERMAL ENERGY↗

First Horizontal Test Results of the HZB SRF Photoinjector for BERLinPro

The BERLinPro project, a small superconducting RF (SRF) c.w. energy recovery linac (ERL) is being built at Helmholtz-Zentrum Berlin in order to develop the technology required for operation of a high current, 100 mA, 50 MeV ERL. The electron source for the accelerator is a 1.4 cell SRF photoinjector fitted with a multi-alkali photocathode. As part of the HZB photoinjector development program three different SRF photoinjectors will be fabricated and tested. The photoinjector described herein is the second cavity that has been fabricated, and the first photoinjector designed for use with a multi-alkali photocathode. The photoinjector has been built and tested at JLab and subsequently shipped to HZB for testing in the horizontal test cryostat HoBiCaT prior to installation in the photoinjector cryomodule. This cryomodule will be used to measure the photocathode operation in a dedicated experiment called GunLab, the precursor to installation in the BERLinPro hall. This paper will report on the final results of the cavity installed in the helium vessel in the vertical testing dewar at Jefferson Lab as well as the first horizontal test in HoBiCaT

Burrill, A.↗

Interpretation of Fracture Initiation Points by In-Well Low-Frequency Distributed Acoustic Sensing in Horizontal Wells

Summary Low-frequency distributed acoustic sensing (LF-DAS) exploits the optical phase shift of Rayleigh backscatter in fiber-optic cables to obtain distributed measurements of changes in strain and temperature. Fiber-optic cables are often installed for multistage hydraulic fracture diagnostics in horizontal wells. LF-DAS in an untreated well provides far-field strain measurements, while offset wells are hydraulically fractured. Such a configuration is called crosswell LF-DAS sensing. Crosswell LF-DAS measurements have proved useful in diagnosing fracture hits, fracture azimuth, planarity, cluster efficiency, fracture propagation rates, and the dynamic distance to the fracture front. In contrast, in-well LF-DAS is conducted on the actively fractured well. Due to cool fracture fluid being injected at high injection rates, the strain component of the LF-DAS response is largely obscured by temperature changes. In permanent fiber-optic cable installations, distributed temperature sensing (DTS) is often conducted simultaneously with LF-DAS. An opportunity exists to decouple the temperature and strain components of LF-DAS sensors to observe strain changes on in-well LF-DAS. The LF-DAS response is modeled as linearly dependent on strain and temperature changes. Theoretical LF-DAS temperature and strain sensitivity coefficients are derived based on the changes to the index of refraction and length of the fiber. Using the DTS measurements, temperature changes are computed, smoothed, filtered, and compared to the LF-DAS response. Crossplots of the in-well LF-DAS measurements and temperature changes from DTS measurements far from the actively fractured region are used to validate the theoretical sensitivity coefficients. Uncertainty in the temperature component of the LF-DAS response is quantified. The difficulty in corresponding the different spatial and temporal resolutions of the DTS and LF-DAS measurements is overcome by comparing the responses over a moving temporal and spatial window. If the LF-DAS response at the center of the window agrees with the DTS response within uncertainty, the measurement is filtered out. After filtering, the remaining nonzero in-well LF-DAS measurements are due to changes in strain. The data are then visualized in waterfall plots. The results indicate that the theoretical and observed strain and temperature coefficients agree within 10%. After the temperature component of the in-well LF-DAS response is extracted, the remaining nonzero measurements are located primarily within the actively treated region. Locations with peaks in the strain response are interpreted to indicate fracture initiation points. These fracture initiation points are compared with in-well DTS and high-frequency DAS noise measurements across multiple stages to better understand fracture initiation along the horizontal well.

Engineering↗

Initial Assessment for a Generic Horizontal Fissile Solution Vessel with an Electron Beam-based Neutron Generator

The LANL NA-23 program office has provided an extensive design study for a medical isotope (i.e. Molybdenum-99) production facility based on a fissile solution vessel with various accelerator technologies. These concept designs and associated R&D have provided affordable pathways for system optimization for industry counter partner such as SHINE and Global Medial Isotope Supply (GMIS). A horizontal fissile solution vessel design with an electron beam (E-beam) accelerator is proposed and studied with modeling capabilities developed for previous concepts (i.e. a generic vertical fissile solution vessel with a deuterium-tritium (DT) accelerator). An initial system assessment is performed by estimating theoretical Molybdenum-99 production yield with MCNP6.2. A baseline system configuration is constructed based on early design discussions between LANL and GMIS. Since only an initial design concept is available at this stage, all associated system design parameters can be adjusted to fit the best system performance characteristics. In this work, unlike previous vertical system studies, the cooling configuration of the horizontal fissile solution vessel is not considered, and only fission reactions are used to quantify the theoretical product yield. Five parametric design studies are proposed, preliminary results are briefly reported, and extended design studies beyond what is proposed here are also discussed. It is concluded that a comprehensive system analysis via multiphysics coupled calculation developed by LANL should be further conducted for the final design.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Computational Fluid Dynamic Simulation for Electrolyte Flow in Horizontal EP of Nb SRF Elliptical Cavities

An electrolyte flow velocity on an electropolishingsurface affects a material removal rate where higherremoval is observed with a higher velocity of theelectrolyte. In horizontal EP (HEP) of an ellipticalcavity, an electrolyte flow near the equator positionmight remain slow and responsible for a lowerremoval compared to that on the iris and beam pipepositions. In order to understand the flow dynamicon an elliptical cavity surface in the horizontal EPprocess, a computational fluid dynamic simulationwas conducted. This study was conducted on 1.3GHz cavity with different cathodes and acid flowrates. Final aim of the study is to improve the flowuniformity to attain uniform removal especially inmulticell cavities.

Chouhan, V.↗

Fermilab’s horizontal test stand upgrade overview and commissioning

Cryo-assemblies with the low-beta Nb3Sn quadrupoles for the High Luminosity LHC (HL-LHC) upgrade will be tested at Fermilab’s magnet test facility. Total of 10 cryo-assemblies will be delivered to CERN within the US HL-LHC Accelerator Upgrade Project (AUP). The horizontal test stand at Fermilab already has been used for testing the existing LHC inner-triplet quadrupoles, but the stand and corresponding electrical and cryogenic sub-systems were not operational more than a decade. In order to restore the test stand functions and meet the design and test requirements for the high luminosity LHC magnets, the existing horizontal test facility at Fermilab underwent a significant refurbishment of the cryogenic and mechanical components. Most of the upgrades were completed and verified during so called zero-magnet test by late 2020, and then final commissioning of the upgraded test stand was completed during the first cryo-assembly test in 2023. These tests verified all the cryo-mechanical installations, as well as the test stand operations, including controlled cooldown and operation at 1.9 K, the helium recovery capabilities, magnet protection and process controls.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Global horizontal spectral irradiance and module spectral response measurements: an open dataset for PV research

This report describes the creation process and final content of a spectral irradiance dataset for Albuquerque, New Mexico accompanied by a set of spectral response measurements for modules deployed at the same location. The spectral irradiance measurements were made using horizontally mounted spectroradiometers; therefore, they represent global horizontal irradiance. The dataset combines non-continuous spectroradiometer and weather measurements from a two-year period into a single calendar year. The data files are accompanied by extensive metadata as well as example calculations and graphs to demonstrate the potential uses of this database. The spectral response measurements were carried out by the National Renewable Energy Laboratory using 12 commercial silicon modules types that are undergoing long-term evaluation at Sandia National Laboratories in Albuquerque.

14 SOLAR ENERGY↗

Convergent evolution and horizontal gene transfer in Arctic Ocean microalgae

Microbial communities in the world ocean are affected strongly by oceanic circulation, creating characteristic marine biomes. The high connectivity of most of the ocean makes it difficult to disentangle selective retention of colonizing genotypes (with traits suited to biome specific conditions) from evolutionary selection, which would act on founder genotypes over time. The Arctic Ocean is exceptional with limited exchange with other oceans and ice covered since the last ice age. To test whether Arctic microalgal lineages evolved apart from algae in the global ocean, we sequenced four lineages of microalgae isolated from Arctic waters and sea ice. Here we show convergent evolution and highlight geographically limited HGT as an ecological adaptive force in the form of PFAM complements and horizontal acquisition of key adaptive genes. Notably, ice-binding proteins were acquired and horizontally transferred among Arctic strains. A comparison with Tara Oceans metagenomes and metatranscriptomes confirmed mostly Arctic distributions of these IBPs. The phylogeny of Arctic-specific genes indicated that these events were independent of bacterial-sourced HGTs in Antarctic Southern Ocean microalgae.

59 BASIC BIOLOGICAL SCIENCES↗

Evolution of Phototrophy in the Chloroflexi Phylum Driven by Horizontal Gene Transfer

The evolutionary mechanisms behind the extant distribution of photosynthesis is a point of substantial contention. Hypotheses range from the presence of phototrophy in the last universal common ancestor and massive gene loss in most lineages, to a later origin in Cyanobacteria followed by extensive horizontal gene transfer into the extant phototrophic clades, with intermediate scenarios that incorporate aspects of both end-members. Here, we report draft genomes of 11 Chloroflexi: the phototrophic Chloroflexia isolate Kouleothrix aurantiaca as well as 10 genome bins recovered from metagenomic sequencing of microbial mats found in Japanese hot springs. Two of these metagenome bins encode photrophic reaction centers and several of these bins form a metabolically diverse, monophyletic clade sister to the Anaerolineae class that we term Candidatus Thermofonsia. Comparisons of organismal (based on conserved ribosomal) and phototrophy (reaction center and bacteriochlorophyll synthesis) protein phylogenies throughout the Chloroflexi demonstrate that two new lineages acquired phototrophy independently via horizontal gene transfer (HGT) from different ancestral donors within the classically phototrophic Chloroflexia class. These results illustrate a complex history of phototrophy within this group, with metabolic innovation tied to HGT. These observations do not support simple hypotheses for the evolution of photosynthesis that require massive character loss from many clades; rather, HGT appears to be the defining mechanic for the distribution of phototrophy in many of the extant clades in which it appears.

Microbiology↗

Assessing the sensitivity of aerosol mass budget and effective radiative forcing to horizontal grid spacing in E3SMv1 using a regional refinement approach

Abstract. Atmospheric aerosols have important impacts on air quality and the Earth–atmospheric energy balance. However, as computing power is limited, Earth system models generally use coarse spatial grids and parameterize finer-scale atmospheric processes. These parameterizations and the simulation of atmospheric aerosols are often sensitive to model horizontal resolutions. Understanding the sensitivities is necessary for the development of Earth system models at higher resolutions with the deployment of more powerful supercomputers. Using the Energy Exascale Earth System Model (E3SM) version 1, this study investigates the impact of horizontal grid spacing on the simulated aerosol mass budget, aerosol–cloud interactions, and the effective radiative forcing of anthropogenic aerosols (ERFaer) over the contiguous United States. We examine the resolution sensitivity by comparing the nudged simulation results for 2016 from the low-resolution model (LR) and the regional refinement model (RRM). As expected, the simulated emissions of natural dust, sea salt, and marine organic matter are substantially higher in the RRM than in the LR. In addition, RRM simulates stronger aqueous-phase production of sulfate through the enhanced oxidation of sulfur dioxide by hydrogen peroxide due to increased cloud liquid water content. In contrast, the gas-phase chemical production of sulfate is slightly suppressed. The RRM resolves more large-scale precipitation and produces less convective precipitation than the LR, leading to increased (decreased) aerosol wet scavenging by large-scale (convective) precipitation. Regarding aerosol effects on clouds, RRM produces larger temporal variabilities in the large-scale liquid cloud fractions than LR, resulting in increased microphysical cloud processing of aerosols (more interstitial aerosols are converted to cloud-borne aerosols via aerosol activation) in RRM. Water vapor condensation is also enhanced in RRM compared to LR. Consequently, the RRM simulation produces more cloud droplets, a larger cloud droplet radius, a higher liquid water path, and a larger cloud optical depth than the LR simulation. A comparison of the present-day and pre-industrial simulations indicates that, for this contiguous United States domain, the higher-resolution increases ERFaer at the top of the model by about 12 %, which is mainly attributed to the strengthened indirect effect associated with aerosol–cloud interactions.

54 ENVIRONMENTAL SCIENCES↗