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At least 217 records · Page 12

Co- and/or post-translational modifications are critical for TCH4 XET activity

TCH4 encodes a xyloglucan endotransglycosylase (XET) of Arabidopsis thaliana. XETs endolytically cleave and religate xyloglucan polymers; xyloglucan is one of the primary structural components of the plant cell wall. Therefore, XET function may affect cell shape and plant morphogenesis. To gain insight into the biochemical function of TCH4, we defined structural requirements for optimal XET activity. Recombinant baculoviruses were designed to produce distinct forms of TCH4. TCH4 protein engineered to be synthesized in the cytosol and thus lack normal co- and post-translational modifications is virtually inactive. TCH4 proteins, with and without a polyhistidine tag, that harbor an intact N-terminus are directed to the secretory pathway. Thus, as predicted, the N-terminal region of TCH4 functions as a signal peptide. TCH4 is shown to have at least one disulfide bond as monitored by a mobility shift in SDS-PAGE in the presence of dithiothreitol (DTT). This disulfide bond(s) is essential for full XET activity. TCH4 is glycosylated in vivo; glycosidases that remove N-linked glycosylation eliminated 98% of the XET activity. Thus, co- and/or post-translational modifications are critical for optimal TCH4 XET activity. Furthermore, using site-specific mutagenesis, we demonstrated that the first glutamate residue of the conserved DEIDFEFL motif (E97) is essential for activity. A change to glutamine at this position resulted in an inactive protein; a change to aspartic acid caused protein mislocalization. These data support the hypothesis that, in analogy to Bacillus beta-glucanases, this region may be the active site of XET enzymes.

NASA Discipline Cell Biology↗

Software Quality Assurance Metrics

Software Quality Assurance (SQA) is a planned and systematic set of activities that ensures conformance of software life cycle processes and products conform to requirements, standards and procedures. In software development, software quality means meeting requirements and a degree of excellence and refinement of a project or product. Software Quality is a set of attributes of a software product by which its quality is described and evaluated. The set of attributes includes functionality, reliability, usability, efficiency, maintainability, and portability. Software Metrics help us understand the technical process that is used to develop a product. The process is measured to improve it and the product is measured to increase quality throughout the life cycle of software. Software Metrics are measurements of the quality of software. Software is measured to indicate the quality of the product, to assess the productivity of the people who produce the product, to assess the benefits derived from new software engineering methods and tools, to form a baseline for estimation, and to help justify requests for new tools or additional training. Any part of the software development can be measured. If Software Metrics are implemented in software development, it can save time, money, and allow the organization to identify the caused of defects which have the greatest effect on software development. The summer of 2004, I worked with Cynthia Calhoun and Frank Robinson in the Software Assurance/Risk Management department. My task was to research and collect, compile, and analyze SQA Metrics that have been used in other projects that are not currently being used by the SA team and report them to the Software Assurance team to see if any metrics can be implemented in their software assurance life cycle process.

McRae, Kalindra A.↗

Lean direct wall fuel injection method and devices

A fuel combustion chamber, and a method of and a nozzle for mixing liquid fuel and air in the fuel combustion chamber in lean direct injection combustion for advanced gas turbine engines, including aircraft engines. Liquid fuel in a form of jet is injected directly into a cylindrical combustion chamber from the combustion chamber wall surface in a direction opposite to the direction of the swirling air at an angle of from about 50.degree. to about 60.degree. with respect to a tangential line of the cylindrical combustion chamber and at a fuel-lean condition, with a liquid droplet momentum to air momentum ratio in the range of from about 0.05 to about 0.12. Advanced gas turbines benefit from lean direct wall injection combustion. The lean direct wall injection technique of the present invention provides fast, uniform, well-stirred mixing of fuel and air. In addition, in order to further improve combustion, the fuel can be injected at a venturi located in the combustion chamber at a point adjacent the air swirler.

Choi, Kyung J.↗

Space Shuttle Program (SSP) Shock Test and Specification Experience for Reusable Flight Hardware Equipment

As commercial companies are nearing a preliminary design review level of design maturity, several companies are identifying the process for qualifying their multi-use electrical and mechanical components for various shock environments, including pyrotechnic, mortar firing, and water impact. The experience in quantifying the environments consists primarily of recommendations from Military Standard-1540, Product Verification Requirement for Launch, Upper Stage, and Space Vehicles. Therefore, the NASA Engineering and Safety Center (NESC) formed a team of NASA shock experts to share the NASA experience with qualifying hardware for the Space Shuttle Program (SSP) and other applicable programs and projects. Several team teleconferences were held to discuss past experience and to share ideas of possible methods for qualifying components for multiple missions. This document contains the information compiled from the discussions

Larsen, Curtis E.↗

Probing Aircraft Flight Test Hazard Mitigation for the Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Research Team

The Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Project Integration Manager requested in July 2012 that the NASA Engineering and Safety Center (NESC) form a team to independently assess aircraft structural failure hazards associated with the ACCESS experiment and to identify potential flight test hazard mitigations to ensure flight safety. The ACCESS Project Integration Manager subsequently requested that the assessment scope be focused predominantly on structural failure risks to the aircraft empennage raft empennage.

Kelly, Michael J.↗

Probing Aircraft Flight Test Hazard Mitigation for the Alternative Fuel Effects on Contrails and Cruise Emissions (ACCESS) Research Team : Appendices - Volume 2

The Alternative Fuel Effects on Contrails and Cruise Emissions (ACCESS) Project Integration Manager requested in July 2012 that the NASA Engineering and Safety Center (NESC) form a team to independently assess aircraft structural failure hazards associated with the ACCESS experiment and to identify potential flight test hazard mitigations to ensure flight safety. The ACCESS Project Integration Manager subsequently requested that the assessment scope be focused predominantly on structural failure risks to the aircraft empennage (horizontal and vertical tail). This report contains the Appendices to Volume I.

Kelly, Michael J.↗

Gas Turbine Engine Inlet Wall Design

A gas turbine engine has an inlet duct formed to have a shape with a first ellipse in one half and a second ellipse in a second half. The second half has an upstream most end which is smaller than the first ellipse. The inlet duct has a surface defining the second ellipse which curves away from the first ellipse, such that the second ellipse is larger at an intermediate location. The second ellipse is even larger at a downstream end of the inlet duct leading into a fan.

Florea, Razvan Virgil↗

Rotary balance data for a typical single-engine general aviation design for an angle of attack range of 8 deg to 90 deg. 1: Low wing model C

Aerodynamic characteristics obtained in a helical flow environment utilizing a rotary balance located in the Langley spin tunnel are presented in plotted form for a 1/6 scale, single engine, low wing, general aviation model (model C). The configurations tested included the basic airplane and control deflections, wing leading edge and fuselage modification devices, tail designs and airplane components. Data are presented without analysis for an angle of attack range of 8 deg to 90 deg and clockwise and counter clockwise rotations covering an omega b/2v range from 0 to .9.

Mulcay, W. J.↗

Rotary balance data for a typical single-engine general aviation design for an angle-of-attack range of 8 deg to 90 deg. 1: High-wing model B

Aerodynamic characteristics obtained in a rotational flow environment utilizing a rotary balance located in a spin tunnel are presented in plotted form for a 1/6.5 scale, single engine, high wing, general aviation airplane model. The configurations tested included the basic airplane, various wing leading-edge devices, tail designs, and rudder control settings as well as airplane components. Data are presented without analysis for an angle of attack range of 8 deg to 90 deg and clockwise and counter-clockwise rotations covering an omega b/2V range from 0 to 0.85.

Bihrle, W., Jr.↗

Rotary balance data for a typical single-engine general aviation design for an angle-of-attack range of 8 deg to 90 deg. 2: High-wing model A

Aerodynamic characteristics obtained in a rotational flow environment utilizing a rotary balance located in the Langley spin tunnel are presented in plotted form for a 1/5-scale, single-engine, high-wing, general aviation airplane model. The configurations tested included various tail designs and fuselage shapes. Data are presented without analysis for an angle of attack range of 8 to 90 degrees and clockwise and counter-clockwise rotations covering an Omega b/2 v range from 0 to 0.85.

Mulcay, W.↗

Rotary balance data for a typical single-engine general aviation design for an angle-of-attack range of 8 deg to 90 deg. 2: Low-wing model B

Aerodynamic characteristics obtained in a rotational flow environment utilizing a rotary balance located in the spin tunnel are presented in plotted form for a 1/6.5 scale, single engine, low wing, general aviation airplane model. The configurations tested included the basic airplane, various wing leading-edge devices, tail designs, and rudder control settings as well as airplane components. Data are presented without analysis for an angle-of-attack range of 8 deg to 90 deg and clockwise and counter-clockwise rotations covering an (omega)(b)/2V range from 0 to 0.85.

Bihrle, W., Jr.↗

Data engineering systems: Computerized modeling and data bank capabilities for engineering analysis

The Data Engineering System (DES) is a computer-based system that organizes technical data and provides automated mechanisms for storage, retrieval, and engineering analysis. The DES combines the benefits of a structured data base system with automated links to large-scale analysis codes. While the DES provides the user with many of the capabilities of a computer-aided design (CAD) system, the systems are actually quite different in several respects. A typical CAD system emphasizes interactive graphics capabilities and organizes data in a manner that optimizes these graphics. On the other hand, the DES is a computer-aided engineering system intended for the engineer who must operationally understand an existing or planned design or who desires to carry out additional technical analysis based on a particular design. The DES emphasizes data retrieval in a form that not only provides the engineer access to search and display the data but also links the data automatically with the computer analysis codes.

Kopp, H.↗

Method of fabricating a rocket engine combustion chamber

A process for making a combustion chamber for a rocket engine wherein a copper alloy in particle form is injected into a stream of heated carrier gas in plasma form which is then projected onto the inner surface of a hollow metal jacket having the configuration of a rocket engine combustion chamber is described. The particles are in the plasma stream for a sufficient length of time to heat the particles to a temperature such that the particles will flatten and adhere to previously deposited particles but will not spatter or vaporize. After a layer is formed, cooling channels are cut in the layer, then the channels are filled with a temporary filler and another layer of particles is deposited.

Holmes, Richard R.↗

Rotary balance data for a typical single-engine general aviation design for an angle-of-attack range of 8 deg to 90 deg. 2: High-wing model C

Aerodynamic characteristics obtained in a helical flow environment utilizing a rotary balance located in the Langley spin g tunnel are presented in plotted form for a 1/6 scale, single engine, high wing, general aviation model. The configurations tested included the basic airplane and control deflections, wing leading edge devices, tail designs, and airplane components. Data are presented without analysis for an angle of attack range of 8 deg to 90 deg and clockwise and counter clockwise rotations covering a spin coefficient range from 0 to 0.9.

Hultberg, R. S.↗

Space chemical engines technology

The topics presented are covered in viewgraph form. The programmatic objective is to provide the technology necessary to proceed in the late 1990's with development of moderate-thrust LOX/LH2 expander cycle engines for various space transportation applications.

Berkopec, Frank D.↗

Anomaly Detection Techniques with Real Test Data from a Spinning Turbine Engine-Like Rotor

Online detection techniques to monitor the health of rotating engine components are becoming increasingly attractive to aircraft engine manufacturers in order to increase safety of operation and lower maintenance costs. Health monitoring remains a challenge to easily implement, especially in the presence of scattered loading conditions, crack size, component geometry, and materials properties. The current trend, however, is to utilize noninvasive types of health monitoring or nondestructive techniques to detect hidden flaws and mini-cracks before any catastrophic event occurs. These techniques go further to evaluate material discontinuities and other anomalies that have grown to the level of critical defects that can lead to failure. Generally, health monitoring is highly dependent on sensor systems capable of performing in various engine environmental conditions and able to transmit a signal upon a predetermined crack length, while acting in a neutral form upon the overall performance of the engine system.

Abdul-Aziz, Ali↗

Preliminary Tests of Nose- and Side- Entrance Blower Cooling Systems for Radial Engines, Special Report

Two cowling systems intended to reduce the drag and improve the low-speed cooling characteristics of conventional radial engine cowlings were tested in model form to determine the practicability of the methods. One cowling included a blower mounted on the rear face of a large propeller spinner which drew cooling air in through side entrance ducts located behind the equivalent engine orifice plate. The air was passed through the equivalent engine orifice plate from rear to front and out through a slot between the spinner and the engine plate. The blower produced substantially all the power necessary to circulate the cooling air in some cases, so the quantity of air flowing was independent of the air speed, Two types of blowers were used, a centrifugal type and one using airfoil blades which forced the air outward from the center of rotation. The other cowling was similar to the conventional N.A.C.A. cowling except for the addition of a large propeller spinner nose. The spinner was provided with a hole in the nose to admit cooling air and blower blades to increase the pressure for cooling at low speeds. The tests show that with both cowling types the basic drag of the nacelle was reduced substantially below that for the N.A.C.A. cowling by virtue of the better nose shape made possible by the spinner . The drag due to the side-entrance ducts was nearly zero when the openings were closed or when the blower was drawing in a certain quantity of air in proportion to the air speed. The drag increased, however, when air mas allowed to spill from the openings. The nose-entrance blower showed considerable promise as a cooling means although the blower tested was relatively inefficient, owing to the fact that the blower compartments evidently were expanded too rapidly under the conditions imposed. by the design.

Biermann, David↗

NTRE extended life feasibility assessment

Results of a feasibility analysis of a long life, reusable nuclear thermal rocket engine are presented in text and graph form. Two engine/reactor concepts are addressed: the Particle Bed Reactor (PBR) design and the Commonwealth of Independent States (CIS) concept. Engine design, integration, reliability, and safety are addressed by various members of the NTRE team from Aerojet Propulsion Division, Energopool (Russia), and Babcock & Wilcox.

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