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At least 217 records · Page 12

Schneider Springs Fire Study 2023 for Ecosystem Respiration Rates: Surface Water Chemistry and Hydrologic Sensor Data across the Yakima River Basin, Washington, USA (v2)

This dataset supports a broader study examining the drivers of spatial variability in wildfire impacts across the Yakima River Basin. Data provided within this dataset were generated from sample collection across 17 total sites (8 sites affected by a recent wildfire, 9 sites unaffected by a recent wildfire) within multiple rivers throughout the Yakima River Basin in Washington, USA from May-July 2023. Fire affected sites are defined as those affected by the 2021 Schneider Springs Fire, based on the drainage area of the streams being within the 2021 Schneider Springs Fire burn perimeter or not (Figure 1, below). The contents include surface water geochemistry data (dissolved organic carbon; total dissolved nitrogen; total suspended solids); short-term sonde data (specific conductivity; turbidity; pH; chlorophyll A; temperature); stream depth data; stream velocity; manual chamber open channel respiration data; sensor time-series data (oxygen; water pressure; barometric pressure); field metadata (including qualitative information on in stream and river corridor characteristics); and environmental context photos taken in the field. The dataset also includes a summary file of the sensor data and plots of the sensor data. Sensors were only recovered at 15 out of the 17 sites, and not all sensors were recovered at all 15 sites (see Methods section for more details), therefore all data does not exist at all sites. Data from a 2022 study at the same sites, as well as additional sites, can be found at https://data.ess-dive.lbl.gov/view/doi:10.15485/1969566. The data package was originally published in November 2023. It was updated in June 2025 (v2; modified files). See the change history section in the readme for more details. For details on how to navigate data packages generated by this project, see https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About. This dataset is comprised of one folder with field photos and one main data folder with two subfolders. The main data folder consists of (1) file-level metadata; (2) data dictionary; (3) field metadata; (4) field protocol; (5) readme; (6) international generic sample number (IGSN) mapping file; and (7) stream depth and averages. The sensor data subfolder consists of (1) sensor installation methods summary; (2) stream velocity; and (3) six subfolders. The BarotrollAtm (barometric pressure; temperature), DepthHOBO (water pressure; temperature), MantaRiver (specific conductivity; turbidity; pH; chlorophyll A; temperature), EXO (specific conductivity; pH; temperature), miniDOT (dissolved oxygen; temperature), and miniDOTManualChamber (dissolved oxygen; temperature) contain time-series data, plots, and summary files. The sample data subfolder consists of (1) total suspended solids (TSS) data; (2) dissolved organic carbon (DOC, measured as non-purgeable organic carbon, NPOC) data and averages; (3) total dissolved nitrogen (TN) data and averages; and (4) methods codes. All files are .csv, .pdf, .jpg, .jpeg, or .mov.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux FLUXNET-1F US-LS2 San Pedro River Lewis Springs Savanna

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-LS2 San Pedro River Lewis Springs Savanna. This is the FLUXNET version of the carbon flux data for the site US-LS2 San Pedro River Lewis Springs Savanna produced by applying the standard ONEFlux (1F) software. Site Description - A riparian savanna located on an old alluvial terrace about 0.5 km from the banks of the entrenched San Pedro River in southeastern Arizona USA. Site climate is semiarid with monsoonal (Jul-Sep) rainfall. The trees access groundwater (depth of ~7 m)from an alluvial aquifer recharged elsewhere. Therefore, site water balance is not closed and ET greatly exceeds precipitation. The perennial bunchgrasses use primarily rainfall. This groundwater-using ecosystem is far more ebulliant and productive than the upland vegetation around it (shrublands and grasslands) outside of the San Pedro River corridor.

Scott, Russell↗

AmeriFlux FLUXNET-1F US-LS1 San Pedro River Lewis Springs Sacaton Grassland

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-LS1 San Pedro River Lewis Springs Sacaton Grassland. This is the FLUXNET version of the carbon flux data for the site US-LS1 San Pedro River Lewis Springs Sacaton Grassland produced by applying the standard ONEFlux (1F) software. Site Description - This is a riparian grassland located along an old alluvial terrace along the banks of the entrenched San Pedro River in southeastern Arizona. Site climate is semiarid with monsoonal (Jul-Sep) rainfall. The grasses access groundwater (depth of 2-4 m)from an alluvial aquifer recharged elsewhere. Site water balance is not closed and ET greatly exceeds precipitation. The perennial bunchgrasses use both rainfall, groundwater and occasional flood water. This groundwater-using ecosystem is far more ebulliant and productive than the upland vegetation around it (shrublands and grasslands) outside of the San Pedro River corridor.

Scott, Russell [USDA-ARS]↗

Evaluating the Effects of the Kingston Fly Ash Release on Fish Reproduction: Spring 2009 – 2010 Studies

On December 22, 2008, a dike containing fly ash and bottom ash at the Tennessee Valley Authority's (TVA) Kingston Fossil Plant in East Tennessee failed and released a large quantity of ash into the adjacent Emory River. Ash deposits from the spill extended 4 miles upstream of the facility to Emory River mile 6 and downstream to Tennessee River mile 564 ({approx}8.5 miles downstream of the confluence of the Emory River with the Clinch River, and {approx}4 miles downstream of the confluence of the Clinch River with the Tennessee River). A byproduct of coal combustion, fly ash contains a variety of metals and other elements which, at sufficient concentrations and in specific forms, can be harmful to biological systems. The ecological effects of fly ash contamination on exposed fish populations depend on the magnitude and duration of exposure, with the most significant risk considered to come from elevated levels of certain metals in the ash, particularly selenium, on fish reproduction and fish early life stages (Lemly 1993; Besser and others 1996). The ovaries of adult female fish in a lake contaminated by coal ash were reported to have an increased frequency of atretic oocytes (dead or damaged immature eggs) and reductions in the overall numbers of developing oocytes (Sorensen 1988) associated with elevated body burdens of selenium. Larval fish exposed to selenium through maternal transfer of contaminants to developing eggs in either contaminated bodies of water (Lemly 1999) or in experimental laboratory exposures (Woock and others 1987, Jezierska and others 2009) have significantly increased incidences of developmental abnormalities. Contact of fertilized eggs and developing embryos to ash in water and sediments may also pose an additional risk to the early life stages of exposed fish populations through direct uptake of metals and other ash constituents (Jezierska and others 2009). The establishment and maintenance of fish populations is intimately associated with the ability of individuals within a population to reproduce. Reproduction is thus generally considered to be the most critical life function affected by environmental contamination. From a regulatory perspective, the issue of potential contaminant-related effects on fish reproduction from the Kingston fly ash spill has particular significance because the growth and propagation of fish and other aquatic life is a specific classified use of the affected river systems. To address the potential effects of fly ash from the Kingston spill on the reproductive health of exposed fish populations, ORNL has undertaken a series of studies in collaboration with TVA that include: (1) a combined field study of metal bioaccumulation in ovaries and other fish tissues (Adams and others 2012) and the reproductive condition of sentinel fish species in reaches of the Emory and Clinch Rivers affected by the fly ash spill (the current report); (2) laboratory tests of the potential toxicity of fly ash from the spill area on fish embryonic and larval development (Greeley and others 2012); (3) additional laboratory experimentation focused on the potential effects of long-term exposures to fly ash on fish survival and reproductive competence (unpublished); and (4) a combined field and laboratory study examining the in vitro developmental success of embryos and larvae obtained from fish exposed in vivo for over two years to fly ash in the Emory and Clinch Rivers (unpublished). The current report focuses on the reproductive condition of adult female fish in reaches of the Emory and Clinch Rivers influenced by the fly ash spill at the beginning of the spring 2009 breeding season - the first breeding season immediately following the fly ash release - and during the subsequent spring 2010 breeding season. Data generated from this and related reproductive/early life stage studies provide direct input to ecological risk assessment efforts and complement and support other phases of the overall biomonitoring program associated with the fly ash spill.

01 COAL, LIGNITE, AND PEAT↗

Compliance Monitoring of Yearling Chinook Salmon and Juvenile Steelhead Survival and Passage at Bonneville Dam, Spring 2011

The purpose of this compliance study was to estimate dam passage survival of yearling Chinook salmon and steelhead smolts at Bonneville Dam during spring 2011. Under the 2008 Federal Columbia River Power System Biological Opinion (BiOp), dam passage survival should be greater than or equal to 0.96 and estimated with a standard error (SE) less than or equal to 0.015. The study also estimated smolt passage survival from the forebay 2 km upstream of the dam to the tailrace 1 km below the dam, as well as the forebay residence time, tailrace egress, and spill passage efficiency, as required in the Columbia Basin Fish Accords. A virtual/paired-release design was used to estimate dam passage survival at Bonneville Dam. The approach included releases of acoustic-tagged smolts above Bonneville Dam that contributed to the formation of a virtual release at the face of the dam. A survival estimate from this release was adjusted by a paired release below Bonneville Dam. A total of 7692 yearling Chinook salmon and 7766 steelhead smolts were tagged and released during the study. The Juvenile Salmon Acoustic Telemetry System (JSATS) tag model number ATS-156dB, weighing 0.438 g in air, was used in this investigation. The high flows during spring 2011 disrupted planned 100 kcfs spill operations at Bonneville Dam. Therefore, dam passage survival was estimated for the early part of the study (i.e., 30 April–13 May) when spill was about 100 kcfs and for the entire season, which included much higher spill levels from 18–31 May 2011.

Skalski, J. R.↗

Final Scientific/Technical Report – DE-EE0002960 Recovery Act. Detachment faulting and Geothermal Resources - An Innovative Integrated Geological and Geophysical Investigation of Pearl Hot Spring, Nevada

The Pearl Host Spring Geothermal Project funded by the DoE Geothermal Program was a joint academic (KU/UT & OU) and industry collaboration (Sierra and Ram Power) to investigate structural controls and the importance of low-angle normal faults on geothermal fluid flow through a multifaceted geological, geophysical, and geochemical investigation in west-central Nevada. The study clearly showed that the geothermal resources in Clayton Valley are controlled by the interplay between low-angle normal faults and active deformation related to the Walker Lane. The study not only identified potentially feasible blind geothermal resource plays in eastern Clayton Valley, but also provide a transportable template for exploration in the area of west-central Nevada and other regional and actively-deforming releasing fault bends. The study showed that deep-seated low-angle normal faults likely act as crustal scale permeability boundaries and could play an important role in geothermal circulation and funneling geothermal fluid into active fault zones. Not unique to this study, active deformation is viewed as an important gradient to rejuvenated fracture permeability aiding the long-term viability of blind geothermal resources. The technical approach for Phase I included the following components, (1) Structural and geological analysis of Pearl Hot Spring Resource, (2) (U-Th)/He thermochronometry and geothermometry, (3) detailed gravity data and modeling (plus some magnetic and resistivity), (4) Reflection and Refraction Seismic (Active Source), (5) Integration with existing and new geological/geophysical data, and (6) 3-D Earth Model, combining all data in an innovative approach combining classic work with new geochemical and geophysical methodology to detect blind geothermal resources in a cost-effective fashion.

15 GEOTHERMAL ENERGY↗

Business Metrics for High-Performance Homes: A Colorado Springs Case Study

The building industry needs to understand how energy ratings can impact homebuilders. Of interest is how energy efficiency may or may not have a positive impact on homebuilders’ business success. Focusing on Colorado Springs, Colorado, as a case study, the U.S. Department of Energy’s Building America research team IBACOS suggests a win–win between a builder’s investment in energy efficiency and that builder’s ability to sell homes. Although this research did not ultimately determine why a correlation may exist, a builder’s investment in voluntary energy-efficiency programs correlated with that builder’s ability to survive the Great Recession of 2007 to 2009. This report explores the relationship between energy-efficiency ratings and the market performance of several builders in Colorado Springs.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Self-Assembling Biological Springs Force Transducers on the Micron Nanoscale

In this project, we are developing a new system for measuring forces within and between nanoscale biological molecules based on mesoscopic springs made of cholesterol helical ribbons. These ribbons self-assemble in a wide variety of complex fluids containing sterol, a mixture of surfactants and water [1] and have spring constants in the range from 0.5 to 500 pN/nm [2-4]. By the end of this project, we have demonstrated that the cholesterol helical ribbons can be used for measuring forces between biological objects and for mapping the strain fields in hydrogels.

59 BASIC BIOLOGICAL SCIENCES↗

Integrated characterization of CO 2 storage reservoirs on the Rock Springs Uplift combining geomechanics, geochemistry, and flow modeling

This project improves understanding of the effects CO 2 injection and storage on geomechanical, petrophysical, and other properties on two potential reservoirs, the Madison Limestone and Weber Sandstone, at the Rock Springs Uplift, southwest Wyoming. Rock physics studies, laboratory experiments, and computer simulations were employed. Two groups of rock samples were prepared, one aged with formation brine and the second aged with CO 2 -saturated formation brine and subsequently used for geomechanical, geochemical, and petrophysical testing. Rock physics studies provided a facies classification to discriminate among different lithologies and to investigate the feasibility of predicting petrophysical properties using seismic inversion. By applying a zone-wise regression relation established in this facies analysis, a 3D reservoir model was developed. It was determined that time-lapse seismic data are not feasible for monitoring CO 2 displacement at the Rock Springs Uplift or to mitigate leakage-risks situations because pressure and fluid saturation have small effects on elastic velocities and synthetic seismograms. The small variations that do occur are due to the low porosity of the sandstone and the high stiffness of the dolomite. In the Madison Limestone, mineral dissolution due to reaction with CO 2 -saturated brine was observed in highly permeable pathways while mineral precipitation happened in intergranular or micropores. Changes in pore size were quantified via Time-Domain Nuclear Magnetic Resonance transverse relaxation time (T 2 ) and diffusion coefficient distributions. As opposed to continuous injection of CO 2 -saturated brine, a subtle increase in porosity after static ‘soaking’ with CO 2 -saturated brine increases permeability due to the heterogeneity of changes in pore and pore throat sizes. Porosity-permeability relationships are fitted using a power-law function that can be adopted to model time-evolved porosity and permeability in a transport-limited CO 2 injection process. The effect of CO 2 -saturated brine on the linear elastic properties of Weber Sandstone and Madison Limestone cannot be generally concluded. Considering horizontal and vertical orientations and different confining pressures, no consistent relationship between stress (or strain) data and reaction with CO 2 -saturated brine was observed in the nonlinear plastic and post-failure regime. The change of elastic constants due to reaction with CO 2 -saturated brine is more significant in Madison Limestone than in Weber Sandstone; however, no consistent trend was observed. Reservoir simulations suggest that injectivity for Weber Sandstone is adversely impacted by low permeability. Changes in confining pressure do not impact injection rate and can be ignored while the effect of changes to pore pressure cannot. The effect of confining pressure and pore pressure on the size of the CO 2 plume in the Madison Limestone is negligible because of higher permeability. Geomechanical and petrophysical properties induced by CO 2 -brine-rock reactions were time-dependent. Simulations considered these time-dependent properties and indicate that injectivity, porosity distribution, pressure distribution and CO 2 saturation were affected.

58 GEOSCIENCES↗

Behavior, Distribution, and Passage Metrics of Juvenile Chinook Salmon Above and Below Lookout Point Dam, Fall 2016 and Spring 2017 (Final Report)

This report presents the results of a fish behavior, distribution, and passage study conducted by the Pacific Northwest National Laboratory (PNNL) for the U.S. Army Corps of Engineers Portland District (USACE) at Lookout Point Dam (or Lookout) during the fall of 2016 (October–December) and spring of 2017 (March–June). The goal of this study was to provide biologists, engineers, resource managers, and regional decision-makers with information about the behavior, distribution, and passage of juvenile Chinook salmon (Oncorhynchus tshawytscha) through the reservoir and tailwaters of Lookout. The results of this study are intended to inform decision-making about long-term structural and operational measures to help rebuild populations of Upper Willamette River spring Chinook salmon listed as threatened under the Endangered Species Act.

59 BASIC BIOLOGICAL SCIENCES↗

Structure, mineralogy, and microbial diversity of geothermal spring microbialites associated with a deep oil drilling in Romania

Modern mineral deposits play an important role in evolutionary studies by providing clues to the formation of ancient lithified microbial communities. Here we report the presence of microbialite-forming microbial mats in different microenvironments at 32°C, 49°C, and 65°C around the geothermal spring from an abandoned oil drill in Ciocaia, Romania. The mineralogy and the macro- and microstructure of the microbialites were investigated, together with their microbial diversity based on a 16S rRNA gene amplicon sequencing approach. The calcium carbonate is deposited mainly in the form of calcite. At 32°C and 49°C, the microbialites show a laminated structure with visible microbial mat-carbonate crystal interactions. At 65°C, the mineral deposit is clotted, without obvious organic residues. Partial 16S rRNA gene amplicon sequencing showed that the relative abundance of the phylum Archaea was low at 32°C (<0.5%) but increased significantly at 65°C (36%). The bacterial diversity was either similar to other microbialites described in literature (the 32°C sample) or displayed a specific combination of phyla and classes (the 49°C and 65°C samples). Bacterial taxa were distributed among 39 phyla, out of which 14 had inferred abundances >1%. The dominant bacterial groups at 32°C were Cyanobacteria, Gammaproteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, Thermi, Actinobacteria, Planctomycetes, and Defferibacteres. At 49°C, there was a striking dominance of the Gammaproteobacteria, followed by Firmicutes, Bacteroidetes, and Armantimonadetes. The 65°C sample was dominated by Betaproteobacteria, Firmicutes, [OP1], Defferibacteres, Thermi, Thermotogae, [EM3], and Nitrospirae. Lastly, several groups from Proteobacteria and Firmicutes, together with Halobacteria and Melainabacteria were described for the first time in calcium carbonate deposits. Overall, the spring from Ciocaia emerges as a valuable site to probe microbes-minerals interrelationships along thermal and geochemical gradients.

54 ENVIRONMENTAL SCIENCES↗

The molecular dimension of microbial species: 3. Comparative genomics of Synechococcus strains with different light responses and in situ diel transcription patterns of associated putative ecotypes in the Mushroom Spring microbial mat

Genomes were obtained for three closely related strains of Synechococcus that are representative of putative ecotypes (PEs) that predominate at different depths in the 1 mm-thick, upper-green layer in the 60°C mat of Mushroom Spring, Yellowstone National Park, and exhibit different light adaptation and acclimation responses. The genomes were compared to the published genome of a previously obtained, closely related strain from a neighboring spring, and differences in both gene content and orthologous gene alleles between high-light-adapted and low-light-adapted strains were identified. Evidence of genetic differences that relate to adaptation to light intensity and/or quality, CO 2 uptake, nitrogen metabolism, organic carbon metabolism, and uptake of other nutrients were found between strains of the different putative ecotypes. In situ diel transcription patterns of genes, including genes unique to either low-light-adapted or high-light-adapted strains and different alleles of an orthologous photosystem gene, revealed that expression is fine-tuned to the different light environments experienced by ecotypes prevalent at various depths in the mat. This study suggests that strains of closely related PEs have different genomic adaptations that enable them to inhabit distinct ecological niches while living in close proximity within a microbial community.

59 BASIC BIOLOGICAL SCIENCES↗

The molecular dimension of microbial species: 2. Synechococcus strains representative of putative ecotypes inhabiting different depths in the Mushroom Spring microbial mat exhibit different adaptive and acclimative responses to light

Closely related strains of thermophilic Synechococcus were cultivated from the microbial mats found in the effluent channels of Mushroom Spring, Yellowstone National Park (YNP). These strains have identical or nearly identical 16S rRNA sequences but are representative of separate, predicted putative ecotype (PE) populations, which were identified by using the more highly resolving psaA locus and which predominate at different vertical positions within the 1-mm-thick upper-green layer of the mat. Pyrosequencing confirmed that each strain contained a single, predominant psaA genotype. Strains differed in growth rate as a function of irradiance. A strain with a psaA genotype corresponding to a predicted PE that predominates near the mat surface grew fastest at high irradiances, whereas strains with psaA genotypes representative of predominant subsurface populations grew faster at low irradiance and exhibited greater sensitivity to abrupt shifts to high light. The high-light-adapted and low-light-adapted strains also exhibited differences in pigment content and the composition of the photosynthetic apparatus (photosystem ratio) when grown under different light intensities. Cells representative of the different strains had similar morphologies under low-light conditions, but under high-light conditions, cells of lowlight-adapted strains became elongated and formed short chains of cells. Collectively, the results presented here are consistent with the hypothesis that closely related, but distinct, ecological species of Synechococcus occupy different light niches in the Mushroom Spring microbial mat and acclimate differently to changing light environments.

59 BASIC BIOLOGICAL SCIENCES↗

New Whole-House Solutions Case Study: Zero Energy Ready Home Multifamily Project: Mutual Housing at Spring Lake

Building cost effective, high performance homes that provide superior comfort, health, and durability is the goal of the Department of Energy's (DOE's) Zero Energy Ready Homes (ZERH) program. This case study describes the development of a 62-unit multifamily community constructed by nonprofit developer Mutual Housing at the Spring Lake subdivision in Woodland, California. The Spring Lake project is expected to be the first ZERH-certified multifamily project nationwide. Building America team Alliance for Residential Building Innovation worked with Mutual Housing throughout the project. An objective of this project was to gain a highly visible foothold for residential buildings built to the DOE ZERH specification that can be used to encourage participation by other California builders.

ARBI↗

Building America Case Study: Zero Energy Ready Home Multifamily Project: Mutual Housing at Spring Lake, Woodland, California

Building cost effective, high performance homes that provide superior comfort, health, and durability is the goal of the Department of Energy's (DOE's) Zero Energy Ready Homes (ZERH) program. Through Building America research and other innovative programs throughout the country, many of the technical challenges to building to the ZERH standard have been addressed. This case study describes the development of a 62-unit multifamily community constructed by nonprofit developer Mutual Housing at the Spring Lake subdivision in Woodland, CA. The Spring Lake project is expected to be the first ZERH-certified multifamily project nationwide. Building America team Alliance for Residential Building Innovation worked with Mutual Housing throughout the project. The case study discusses challenges encountered, lessons learned, and how obstacles were overcome. An objective of this project was to gain a highly visible foothold for residential buildings built to the DOE ZERH specification that can be used to encourage participation by other California builders.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Flange joint system for SRF cavities utilizing high force spring clamps for low particle generation

A flange joint system for SRF cavities. The flange joint system includes a set of high force spring clamps that produce high force on the simple flanges of Superconducting Radio Frequency (SRF) cavities to squeeze conventional metallic seals. The system establishes the required vacuum and RF-tight seal with minimum particle contamination to the inside of the cavity assembly. The spring clamps are designed to stay within their elastic range while being forced open enough to mount over the flange pair. Upon release, the clamps have enough force to plastically deform metallic seal surfaces and continue to a new equilibrium sprung dimension where the flanges remain held against one another with enough preload such that normal handling will not break the seal.

42 ENGINEERING↗