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Prognosticating Survival in Hepatocellular Carcinoma with Elevated Baseline Alpha-fetoprotein Treated with Radioembolization Using a Novel Laboratory Scoring System: Initial Development and Validation

Aims: To investigate laboratory parameters as predictors of overall survival (OS) for hepatocellular carcinoma (HCC) treated with radioembolization and develop/validate a scoring system. Methods: With IRB approval, we included all patients with baseline alpha-fetoprotein (AFP) > 100 ng/dL from our prospectively acquired HCC radioembolization database. Neutrophil–lymphocyte ratio, albumin–bilirubin (ALBI), and AFP were measured at baseline and at 1-, 3-, and 6-month post-radioembolization Landmarks. OS was assessed from these Landmarks. Univariate/multivariate analyses were performed to evaluate OS predictability of these parameters. Baseline Imaging, Laboratory, and Combination scoring systems were developed. Developing/validating groups were created to investigate/validate the score’s OS predictability. Time-dependent receiver operating characteristics (ROC) were evaluated. Patients were stratified into groups I, II, and III by using 25th and 75th percentile cutoffs according to change in Laboratory Score from baseline. Results: 345/401 (86%), 238/401 (59%), and 167/401 (42%) patients had laboratory parameters available at the 1-, 3-, and 6-month Landmarks, respectively. ALBI and AFP were significant OS prognosticators at all Landmarks. The Laboratory Score [ALBI + (0.3 × LnAFP)] was developed/internally validated to predict OS from these Landmarks. Areas under the curve of time-dependent ROCs of the Baseline Imaging vs. Laboratory scores in predicting patient OS post 3 and 6 months Landmarks were 0.56 versus 0.82 and 0.57 versus 0.77, respectively. OS differences in groups I, II, and III according to change in Laboratory Score from baseline were significant (p < 0.001). Conclusions: Post-radioembolization AFP and ALBI scores were significant OS prognosticators. A decrease in post-therapeutic Laboratory Score, which combines AFP and ALBI, correlates with an improved OS.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Enhancing laboratory capacity during Ebola virus disease (EVD) heightened surveillance in Liberia: lessons learned and recommendations

Following a declaration by the World Health Organization that Liberia had successfully interrupted Ebola virus transmission on May 9th, 2015; the country entered a period of enhanced surveillance. The number of cases had significantly reduced prior to the declaration, leading to closure of eight out of eleven Ebola testing laboratories. Enhanced surveillance led to an abrupt increase in demand for laboratory services. We report interventions, achievements, lessons learned and recommendations drawn from enhancing laboratory capacity. Using archived data, we reported before and after interventions that aimed at increasing laboratory capacity. Laboratory capacity was defined by number of laboratories with Ebola Virus Disease (EVD) testing capacity, number of competent staff, number of specimens tested, specimen backlog, daily and surge testing capacity, and turnaround time. Using Stata 14 (Stata Corporation, College Station, TX, USA), medians and trends were reported for all continuous variables. Our results show that between May and December 2015, interventions including recruitment and training of eight staff, establishment of one EVD laboratory facility, implementation of ten Ebola GeneXpert diagnostic platforms, and establishment of working shifts yielded an 8-fold increase in number of specimens tested, a reduction in specimens backlog to zero, and restoration of turn-around time to 24 hours. This enabled a more efficient surveillance system that facilitated timely detection and containment of two EVD clusters observed thereafter. In conclusion, effective enhancement of laboratory services during high demand periods requires a combination of context-specific interventions. Building and ensuring sustainability of local capacity is an integral part of effective surveillance and disease outbreak response efforts.

60 APPLIED LIFE SCIENCES↗

Laboratory Experiments and Instrument Intercomparison Studies of Carbonaceous Aerosol Particles

Aerosols containing black carbon (and some specific types of organic particulate matter) directly absorb incoming light, heating the atmosphere. In addition, all aerosol particles backscatter solar light, leading to a net-cooling effect. Indirect effects involve hydrophilic aerosols, which serve as cloud condensation nuclei (CCN) that affect cloud cover and cloud stability, impacting both atmospheric radiation balance and precipitation patterns. At night, all clouds produce local warming, but overall clouds exert a net-cooling effect on the Earth. The effect of aerosol radiative forcing on climate may be as large as that of the greenhouse gases, but predominantly opposite in sign and much more uncertain. The uncertainties in the representation of aerosol interactions in climate models makes it problematic to use model projections to guide energy policy. The objective of our program is to reduce the uncertainties in the aerosol radiative forcing in the two areas highlighted in the ASR Science and Program Plan. That is, (1) addressing the direct effect by correlating particle chemistry and morphology with particle optical properties (i.e. absorption, scattering, extinction), and (2) addressing the indirect effect by correlating particle hygroscopicity and CCN activity with particle size, chemistry, and morphology. In this connection we are systematically studying particle formation, oxidation, and the effects of particle coating. The work is specifically focused on carbonaceous particles where the uncertainties in the climate relevant properties are the highest. The ongoing work consists of laboratory experiments and related instrument inter-comparison studies both coordinated with field and modeling studies, with the aim of providing reliable data to represent aerosol processes in climate models. The work is performed in the aerosol laboratory at Boston College. At the center of our laboratory setup are two main sources for the production of aerosol particles: (a) two well-characterized source of soot particles and (b) a flow reactor for controlled OH and/or O3 oxidation of relevant gas phase species to produce well-characterized SOA particles. After formation, the aerosol particles are subjected to physical and chemical processes that simulate aerosol growth and aging. A suite of instruments in our laboratory is used to characterize the physical and chemical properties of aerosol particles before and after processing. The Time of Flight Aerosol Mass Spectrometer (ToF-AMS) together with a Scanning Mobility Particle Sizer (SMPS) measures particle mass, volume, density, composition (including black carbon content), dynamic shape factor, and fractal dimension. The–ToF-AMS was developed at ARI with Boston College participation. About 120 AMS instruments are now in service (including 5 built for DOE laboratories) performing field and laboratory studies world-wide. Other major instruments include a thermal denuder, two Differential Mobility Analyzers (DMA), a Cloud Condensation Nuclei Counter (CCN), a Thermal desorption Aerosol GC/MS (TAG) and the new Soot Particle Aerosol Mass Spectrometer (SP-AMS). Optical instrumentation required for the studies have been brought to our laboratory as part of ongoing and planned collaborative projects with colleagues from DOE, NOAA and university laboratories. Optical instruments that will be utilized include a Photoacoustic Spectrometer (PAS), a Cavity Ring Down Aerosol Extinction Spectrometer (CRD-AES), a Photo Thermal Interferometer (PTI), a new 7-wavelength Aethalometer and a Cavity Attenuated Phase Shift Extinction Monitor (CAPS). These instruments are providing aerosol absorption, extinction and scattering coefficients at a range of atmospherically relevant wavelengths. During the past two years our work has continued along the lines of our original proposal. We report on 12 completed and/or continuing projects conducted during the period 08/14 to 0814/2015. These projects are described in 17 manuscripts published in refereed journals.

54 ENVIRONMENTAL SCIENCES↗

Interim Report of the Commission to Review the Effectiveness of the National Energy Laboratories

The Commission to Review the Effectiveness of the National Energy Laboratories was charged by Congress in January 2014 to evaluate the mission, capabilities, size, performance, governance, and agency oversight of the 17 Department of Energy (DOE) laboratories. Given the incredibly broad scope and aggressive timeline (the original deadline was February 2015), the Secretary of Energy and Congress agreed to split the task into two phases. This interim report contains the preliminary observations and recommendations gleaned from Phase 1 of the study, which consisted of a literature review; visits to five of the National Laboratories; semi-structured interviews with staff from across the National Laboratories, DOE, other Federal agencies, companies, other non-governmental organizations, and additional interested parties; and presentations at monthly public Commission meetings. The Commission notes that the purpose of the National Laboratories is to provide critical capabilities and facilities in service of DOE’s mission and the needs of the broader national and international science and technology (S&T) community, including other Federal agencies, academia, and private industry. The National Laboratories are successfully fulfilling that mission today. While the Commission believes significant improvements can be made to many aspects of DOE management and governance of the laboratories, those issues do not detract from the National Laboratories’ remarkable contributions to the American public. In Phase 2 the Commission will focus on ways to make the process of carrying out their missions more efficient and effective.

99 GENERAL AND MISCELLANEOUS↗

FRMAC Laboratory Analysis RadResponder Gap Analysis and Web Portal Enhancements

The Federal Radiological Monitoring and Assessment Center (FRMAC) relies on accurate and defensible analytical laboratory data to support its mission. FRMAC Laboratory Analysis personnel are responsible for (1) receiving samples, (2) managing samples, and (3) providing data quality assurance. Currently, the RadResponder software application does not meet all these needs. With some modifications, RadResponder could meet the needs for sample receiving functions, but it does not meet the needs of sample management and data quality assurance functions. The FRMAC Laboratory Analysis team has discussed and reviewed the following options moving forward: Option 1: Make minor revisions to RadResponder to improve sample receiving capability, purchase and configure a commercial laboratory information management system (LIMS) to perform sample management and data quality assurance, and build an interface between RadResponder and the commercial-off-the-shelf LIMS. Option 2: Make major revisions to RadResponder for all FRMAC Laboratory Analysis functions to support required sample management and data quality assurance activities. Option 3: Create a custom-built LIMS system to interface with RadResponder. Note: All three options will require the development of a Laboratory Analysis web portal and will require funding for ongoing maintenance and training. The FRMAC Laboratory Analysis team highly recommends Option 1 as the best and most efficient path forward. Commercial-off-the-shelf LIMS products have been proven successful in the laboratory community for decades. Option 1 leverages these proven technologies and takes advantage of RadResponder's current strengths.

61 RADIATION PROTECTION AND DOSIMETRY↗

Oak Ridge National Laboratory Roane and Anderson Counties, Tennessee Historic Architectural Resource Survey June 2018

From February through September 2017, Cultural Resource Analysts, Inc. personnel completed a historic architectural resource survey of the Oak Ridge National Laboratory in Roane and Anderson Counties, Tennessee. The survey was completed at the request of UT-Battelle, LLC, the private not-for-profit company that manages and operates Oak Ridge National Laboratory for the U.S. Department of Energy. The purpose of the survey was to assist the Department of Energy in complying with the National Historic Preservation Act of 1966, as amended, by providing updated recommendations regarding the National Register of Historic Places eligibility of historic architectural resources at Oak Ridge National Laboratory. The survey included the entirety of Oak Ridge National Laboratory’s main campus that occupies approximately 1,100 acres in Bethel and Melton Valleys and on Chestnut Ridge. Based on Facilities Information Management System data and guidance provided by UT-Battelle, LLC, preservation and modernization staff, the survey team developed a list of resources for inclusion in the survey. The list included all substantial buildings constructed in or before 1980 in order to provide sufficient coverage of buildings that will turn 50 years of age within the next decade. Only those buildings with recorded area over 100 sq ft were included in the survey. Additionally, the UT-Battelle team identified 15 buildings for more intensive survey because such buildings are central to Oak Ridge National Laboratory’s future mission needs and are expected to undergo renovations in the foreseeable future. These building were subject to both exterior and interior documentation with particular focus on identifying character-defining features, and thus were subject to more detailed analysis than the other resources included in the survey. The findings of this historic resource survey build on the conclusions of the 1994 survey by DuVall & Associates, Inc., as well as the survey updates completed by Thomason and Associates in 2004 and 2015 in support of the Oak Ridge National Laboratory Historic Preservation Plan. Within 12 distinct areas of Oak Ridge National Laboratory, as well as outlying areas, the survey recorded 195 buildings and structures constructed in or before 1980. In consideration of the role and significance of each area and each individual resource within the context of Oak Ridge National Laboratory, and in light of their current integrity and condition, each recorded resource was evaluated to determine if it is eligible for listing in the National Register of Historic Places.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Laboratory Directed Research and Development Program: FY 2023 Completed Projects Report

Oak Ridge National Laboratory (ORNL) is the US Department of Energy’s (DOE’s) largest multiprogram science, technology, and energy laboratory. It possesses distinctive capabilities in a variety of fields, such as neutron science, computing, advanced materials, and nuclear science and technology. Using these capabilities, ORNL conducts basic and applied research and development (R&D) to support DOE’s overarching mission “to ensure America’s security and prosperity by addressing its energy, environmental and nuclear challenges through transformative science and technology solutions.” As a national resource, ORNL also applies its capabilities and skills to the specific needs of other federal agencies and customers through the DOE Strategic Partnership Projects (SPP) Program. Information about the laboratory and its programs is available on the ORNL website. The Laboratory Directed Research and Development (LDRD) Program at ORNL operates under the authority of the DOE Order 413.2C, Laboratory Directed Research and Development, which establishes DOE’s requirements for the program while providing the laboratory director broad flexibility for program implementation. The LDRD Program funds are obtained through a charge to all laboratory programs. Although it represents a relatively small portion of the overall research budget, the LDRD Program plays an essential role in maintaining the laboratory’s ability to respond to national needs. The program allows ORNL to improve its distinctive capabilities and to enhance its ability to conduct cutting-edge R&D. This report provides an overview of the LDRD Program at ORNL in FY 2023 and contains summaries of all the LDRD research projects that concluded between October 1, 2022, and September 30, 2023.

99 GENERAL AND MISCELLANEOUS↗

Assessment of the Idaho National Laboratory Remote Handled Low Level Waste Disposal Facility Hydraulic Performance

This document provides a summary of the expected hydraulic performance of the vault drainage system installed at Idaho National Laboratory’s Remote Handled Low-Level Waste disposal facility. Hydraulic performance was assessed through analysis of the mechanical properties of the drainage system and backfill materials, including material gradation and proctor data; laboratory data, including bulk density, porosity, unsaturated hydraulic conductivity, moisture-retention data, and van Genuchten parameters; and field-data collected using in-situ instrumentation with data collected during a vault-scale infiltration characterization test. Hydraulic performance of the vault drainage system was designed to promote a greater than 500-year vault concrete longevity. The predicted hydraulic performance is, in part, a function of the materials used in construction, sequencing of the hydraulic drainage materials, and the as-built condition of the materials following compaction necessary to ensure vault stability. Specifying specific as-built hydraulic properties is exceedingly difficult at the facility specification/design phase. Instead, material mechanical properties were specified and confirmed early during the construction process. As the drainage materials were installed, samples were sent to a laboratory for hydraulic property testing and, after drainage system installation was complete around the performance assessment vaults, a field-scale infiltration test was conducted with data collection to verify the as-built hydraulic performance. This report provides summary data for the measured mechanical properties, laboratory data, and field infiltration characterization test data. Mechanical data have relatively little variability because of the sieve system used when the materials were crushed onsite. Laboratory data were obtained for a relatively small number of material samples, but the data obtained for each sample are extensive. However, laboratory data provide the moisture retention relationships and relative hydraulic conductivity-moisture content relationships for uninstalled materials. Conducting field-scale infiltration tests with collection of moisture retention data and wetting front propagation data allows extension of laboratory data to the as-installed conditions of the vault drainage system. These combined data types are integrated using a numerical simulation of the infiltration characterization test to refine model parameters. The infiltration characterization model will be used to support the hydraulic and concrete performance of the vault system provided in the facility performance assessment.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Teaching Laboratory Renovation

Scientists at universities across Iraq are actively working to report actual incidents and accidents occurring in their laboratories, as well as structural improvements made to improve safety and security, to raise awareness and encourage openness, leading to widespread adoption of robust Chemical Safety and Security (CSS) practices. The improvement of students’ understanding of concepts in science and its applications, practical scientific skills and understanding of how science and scientists work in laboratory experiences have been considered key aspects of education in science for over 100 years. Facility requirements for the necessary level of safety and security combined with specific requirements relevant to the course to be conducted dictate the structural design of a particular laboratory, and the design process must address both. This manuscript is the second in a series of five case studies describing laboratory incidents, accidents, and laboratory improvements. We summarize the process used to guide a major renovation of the chemistry instructional laboratory facilities at Al-Nahrain University and discuss lessons learned from the project.

chemistry laboratory↗

Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models

We are developing a laboratory-scale model to improve our understanding and capacity to assess the biological risks of genetically engineered bacteria and their genetic elements in the natural environment. Our hypothetical scenario concerns an industrial bioreactor failure resulting in the introduction of genetically engineered bacteria to a downstream municipal wastewater treatment plant (MWWTP). As the first step towards developing a model for this scenario, we sampled microbial communities from the aeration basin of a MWWTP at three seasonal time points. Having established a baseline for community composition, we investigated how the community changed when propagated in the laboratory, including cell culture media conditions that could provide selective pressure in future studies. Specifically, using PhyloChip 16S-rRNA-gene targeting microarrays, we compared the compositions of sampled communities to those of inocula propagated in the laboratory in simulated wastewater conditionally amended with various carbon sources (glucose, chloroacetate, D-threonine) or the ionic liquid 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl). Proteobacteria , Bacteroidetes , and Actinobacteria were predominant in both aeration basin and laboratory-cultured communities. Laboratory-cultured communities were enriched in γ- Proteobacteria . Enterobacteriaceae , and Aeromonadaceae were enriched by glucose, Pseudomonadaceae by chloroacetate and D-threonine, and Burkholderiacea by high (50 mM) concentrations of chloroacetate. Microbial communities cultured with chloroacetate and D-threonine were more similar to sampled field communities than those cultured with glucose or [C2mim]Cl. Although observed relative richness in operational taxonomic units (OTUs) was lower for laboratory cultures than for field communities, both flask and reactor systems supported phylogenetically diverse communities. These results importantly provide a foundation for laboratory models of industrial bioreactor failure scenarios.

59 BASIC BIOLOGICAL SCIENCES↗

Report on Sustainability Program Peer Reviews at NNSA Laboratories

In FY 2012 peer reviews were conducted at three of the National Nuclear Security Administration (NNSA) Laboratories on the implementation of sustainability programs to achieve the DOE Strategic Sustainability Performance Plan goals. The three Laboratories selected include Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL), and Sandia National Laboratory (SNL). These reviews were initiated by the LANS/LLNS Board of Governors Business & Operations Committee and were joined by Sandia National Laboratories to get a broad understanding of the challenges and successes experienced at the three Labs. Each review used a common approach and process for inquiry. The peer review process provided a beneficial benchmarking opportunity for each of the Laboratories. The purpose of this report summary is to bring together the mutual challenges faced across the three NNSA national labs to deepen the conversations between the sites and NNSA as the customer to work constructively toward achieving progress on the sustainability goals. The team identified many noteworthy practices and common challenges faced at each of the three sites. At each site the review team also saw a strong connection between the Environmental Management System (EMS) and the implementation of the site’s sustainability program. One common challenge was that in spite of the linkage to the EMS program the implementation efforts to achieve the sustainability goals were perceived as being the sole responsibility of the facilities and infrastructure management organizations vice an expectation for strong employee engagement. Another common challenge was perceived within the procurement organizations and demonstrated effort toward meeting the sustainable acquisition requirements throughout the organization and the various contract instruments used at each site.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Economic Impact of Pacific Northwest National Laboratory on the State of Washington in Fiscal Year 2013

Pacific Northwest National Laboratory (PNNL) is a large economic entity, with $1.06 billion in annual funding, $936 million in total spending, and 4,344 employees in fiscal year (FY) 2013. Four thousand, one hundred and one (4,101) employees live in Washington State. The Laboratory directly and indirectly supports almost $1.31 billion in economic output, 6,802 jobs, and $514 million in Washington State wage income from current operations. The state also gains more than $1.21 billion in output, more than 6,400 jobs, and $459 million in income through closely related economic activities, such as visitors, health care spending, spending by resident retirees, and spinoff companies. PNNL affects Washington’s economy through commonly recognized economic channels, including spending on payrolls and other goods and services that support Laboratory operations. Less-commonly recognized channels also have their own impacts and include company-supported spending on health care for its staff members and retirees, spending of its resident retirees, Laboratory visitor spending, and the economic activities in a growing constellation of “spinoff” companies founded on PNNL research, technology, and managerial expertise. PNNL also has a significant impact on science and technology education and community nonprofit organizations. PNNL is an active participant in the future scientific enterprise in Washington with the state’s K-12 schools, colleges, and universities. The Laboratory sends staff members to the classroom and brings hundreds of students to the PNNL campus to help train the next generation of scientists, engineers, mathematicians, and technicians. This investment in human capital, though difficult to measure in terms of current dollars of economic output, is among the important lasting legacies of the Laboratory. Finally, PNNL contributes to the local community with millions of dollars’ worth of cash and in-kind corporate and staff contributions, all of which strengthen the economy. This report quantifies these effects, providing detailed information on PNNL’s revenues and expenditures, as well as the impacts of its activities on the rest of the Washington State economy. This report also describes the impacts of the four closely related activities: health care spending, spinoff companies with roots in PNNL, visitors to the Laboratory, and PNNL retirees.

99 GENERAL AND MISCELLANEOUS↗

LDRD Highlights at the National Laboratories

To meet the nation’s critical challenges, the Department of Energy (DOE) national laboratories have always pushed the boundaries of science, technology, and engineering. The Atomic Energy Act of 1954 provided the basis for these laboratories to engage in the cutting edge of science and technology and respond to technological surprises, while retaining the best scientific and technological minds. To help re-energize this commitment, in 1991 the U.S. Congress authorized the national laboratories to devote a relatively small percentage of their budget to creative and innovative work that serves to maintain their vitality in disciplines relevant to DOE missions. Since then, this effort has been formally called the Laboratory Directed Research and Development (LDRD) Program. LDRD has been an essential mechanism to enable the laboratories to address DOE’s current and future missions with leading-edge research proposed independently by laboratory technical staff, evaluated through expert peer-review committees, and funded by the individual laboratories consistent with the authorizing legislation and the DOE LDRD Order 413.2C.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Air Flow Modeling in the Wind Tunnel of the FHWA Aerodynamics Laboratory at Turner-Fairbank Highway Research Center

Computational fluid dynamics (CFD) modeling is widely used in industry for design and in the research community to support, compliment, and extend the scope of experimental studies. Analysis of transportation infrastructure using high performance cluster computing with CFD and structural mechanics software is done at the Transportation Research and Analysis Computing Center (TRACC) at Argonne National Laboratory. These resources, available at TRACC, were used to perform advanced three-dimensional computational simulations of the wind tunnel laboratory at the Turner-Fairbank Highway Research Center (TFHRC). The goals were to verify the CFD model of the laboratory wind tunnel and then to use versions of the model to provide the capability to (1) perform larger parametric series of tests than can be easily done in the laboratory with available budget and time, (2) to extend testing to wind speeds that cannot be achieved in the laboratory, and (3) to run types of tests that are very difficult or impossible to run in the laboratory. Modern CFD software has many physics models and domain meshing options. Models, including the choice of turbulence and other physics models and settings, the computational mesh, and the solver settings, need to be validated against measurements to verify that the results are sufficiently accurate for use in engineering applications. The wind tunnel model was built and tested, by comparing to experimental measurements, to provide a valuable tool to perform these types of studies in the future as a complement and extension to TFHRC’s experimental capabilities. Wind tunnel testing at TFHRC is conducted in a subsonic open-jet wind tunnel with a 1.83 m (6 foot) by 1.83 m (6 foot) cross section. A three component dual force-balance system is used to measure forces acting on tested models, and a three degree of freedom suspension system is used for dynamic response tests. Pictures of the room are shown in Figure 1-1 to Figure 1-4. A detailed CAD geometry and CFD model of the wind tunnel laboratory at TFHRC was built and tested. Results were compared against experimental wind velocity measurements at a large number of locations around the room. This testing included an assessment of the air flow uniformity provided by the tunnel to the test zone and assessment of room geometry effects, such as influence of the proximity the room walls, the non-symmetrical position of the tunnel in the room, and the influence of the room setup on the air flow in the room. This information is useful both for simplifying the computational model and in deciding whether or not moving, or removing, some of the furniture or other movable objects in the room will change the flow in the test zone.

97 MATHEMATICS AND COMPUTING↗

Laboratory Directed Research and Development Program - FY 2017 ORNL Annual Summary of Completed Projects

This report includes summaries of all ORNL LDRD research activities completed by September 30, 2017. It is sent to the ORNL Technical Officer for export control review and classification review prior to being delivered to the Office of Scientific and Technical Information. LDRD is a relatively small but vital program whose purpose is to meet the DOE mission. It allows ORNL and other DOE laboratories to improve their distinctive capabilities and to enhance their ability to conduct cutting-edge R&D for DOE and SPP sponsors. R&D projects funded through LDRD support the following general goals: maintain the scientific and technical vitality of the Laboratory; enhance the Laboratory’s ability to address future DOE missions; foster creativity and stimulate exploration of forefront areas of science and technology; serve as a proving ground for new concepts in R&D; and support high-risk, potentially high-value R&D. ORNL has established a program with three components to meet its LDRD objectives and to fulfill the particular needs of the Laboratory: the Director’s R&D Fund, the Seed Money Fund, and the Distinguished Fellowship Fund (Householder, Russell, Weinberg, and Wigner Fellowships). As outlined in Table 1, these three funds are complementary. The Director’s R&D Fund develops new capabilities in support of the Laboratory initiatives, the Seed Money Fund is open to all innovative ideas that have the potential for enhancing the Laboratory’s core scientific and technical competencies, and the Distinguished Fellowship Fund allows the Laboratory to hire exceptional early career scientists to refresh its scientific and technical capabilities. Provision for multiple routes of access to ORNL LDRD funds maximizes the likelihood that novel ideas with scientific and technological merit will be recognized and supported.

99 GENERAL AND MISCELLANEOUS↗

Site-wide occupancy assessment using camera traps for seven mammalian species at Los Alamos National Laboratory

Los Alamos National Laboratory (LANL or Laboratory) is committed to solving national security challenges through scientific excellence and has been serving the nation and northern New Mexico for over 70 years. Being located on the Pajarito Plateau in the eastern flanks of the Jemez Mountains, the Laboratory is surrounded by a rich diversity of plants and animals. It is common to see many different species of wildlife on Laboratory property; however, sometimes interactions with wildlife can be negative. Vehicle accidents with wildlife have become a common occurrence. With the current and ongoing expansion of the Laboratory on the Pajarito Plateau, it has the potential to further impact wildlife movement including large game species. Local agencies and tribal Pueblos rely on large game species and do not want these species to be restricted from moving across property boundaries. Temporal and spatial aspects of where wildlife occur on the site is a phenomena that is either not well understood in uncommon species or needs periodic reevaluation for common species. Estimating the distribution of multiple species across the landscape provides wildlife biologists with crucial information for monitoring and conserving animal populations in a particular area. Utilizing motion activated wildlife cameras, also known as camera traps, to monitor wildlife populations has become an essential tool for biologists. Camera traps are non-invasive and cost-effective and can document multiple elusive or uncommon wildlife species, such as carnivores, simultaneously. Occupancy modeling provides a flexible framework for the analysis of the distribution for multiple wildlife species. It explicitly recognizes whether a species is spatially common or rare (occupancy = ψ) and if that species is easy or hard to detect (detection probability = p ). Multispecies and multi-season occupancy models can detect trends in species occupancy because individual species may vary in seasonal movements, detection probability, and transition rates between habitats. In this study, we assessed the site as a whole to ascertain when and where medium and large mammal species are present. Understanding wildlife patterns at the Laboratory will better inform future management decisions regarding land use and development strategies. We placed motion activated wildlife cameras in a random systematic sampling design and used these data to create occupancy models. We tested for differences in single-species occupancy and detection probability by season of mammal species captured on 20 camera traps placed across the Laboratory in a 40 mi² (103 km²) area. We focus the interpretation of our findings on seven mammal species found during this study. They are Rocky Mountain elk ( Cervus canadensis nelsoni ; hereafter “elk”), mule deer ( Odocoileus hemionus ; hereafter “deer”), mountain lion ( Puma concolor ; hereafter “lion”), American black bear ( Ursus americanus ; hereafter “bear”), coyote ( Canis latrans ), bobcat ( Lynx rufus ), and gray fox ( Urocyon cinereoargenteus ; hereafter “fox”).

59 BASIC BIOLOGICAL SCIENCES↗

Laboratory Directed Research and Development Program (FY 2019 Annual Summary of Completed Projects)

Oak Ridge National Laboratory (ORNL) is the US Department of Energy’s (DOE’s) largest multiprogram science, technology, and energy laboratory with distinctive capabilities in neutron science, computing, advanced materials, and nuclear science and technology. Using these capabilities, ORNL conducts basic and applied research and development (R&D) to support DOE’s overarching mission to “ensure America’s security and prosperity by addressing its energy, environmental and nuclear challenges through transformative science and technology solutions.” As a national resource, ORNL also applies its capabilities and skills to specific needs of other federal agencies and customers through the DOE Strategic Partnership Projects (SPP) program. Information about the laboratory and its programs is available at the ORNL website. The Laboratory Directed Research and Development (LDRD) program at ORNL operates under the authority of the DOE Order 413.2C, “Laboratory Directed Research and Development,” which establishes DOE’s requirements for the program while providing the laboratory director broad flexibility for program implementation. The LDRD program funds are obtained through a charge to all laboratory programs. ORNL reports on the program status to DOE each year.

99 GENERAL AND MISCELLANEOUS↗

PPPL Laboratory Directed Research and Development (Project Final Reports, FY2018 - FY2020)

The U.S. Department of Energy’s (DOE) Princeton Plasma Physics Laboratory (PPPL) is a collaborative national center for fusion energy science, basic sciences, and advanced technology. The Laboratory has three major missions: (1) to develop the scientific knowledge and advanced engineering to enable fusion to power the U.S. and the world; (2) to advance the science of nanoscale fabrication for technologies of tomorrow; and (3) to further the development of the scientific understanding of the plasma universe from laboratory to astrophysical scales. PPPL’s Laboratory Directed Research and Development (LDRD) program supports and encourages creativity and innovation and contributes to its long-term viability. New scientific and technical research areas emerge and are nurtured through the program. Furthermore, new capabilities are developed to enable the Laboratory to meet its and DOE’s missions. The program is used to systematically diversify the Laboratory’s programs and mission. In the last few years, the program has started projects in nanomaterial synthesis, microelectronics, advanced x-ray spectroscopy, high-energy-density physics, superconducting magnet technology, machine learning and artificial intelligence, 3D magnetic fields to optimize fusion plasmas, integration of permanent magnets with simple high-field magnets to reduce the cost of producing complex 3D magnetic fields, advanced computational methods for predictive understanding and control of fusion plasma, development of quantum computing algorithms for plasma physics, liquid metal plasma-facing components for fusion reactors, virtual engineering, and plasma-based space propulsion. The program is also the vehicle to recruit and train talented scientists and engineers with the new skills needed to perform the Laboratory’s mission. Many of the new hires through the program go on to become world-class scientists and engineers in their fields. This report provides descriptions and accomplishments of those LDRD projects that were completed during fiscal years 2018 through 2020.

36 MATERIALS SCIENCE↗