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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 217 records · Page 12

Transient dissociation of polyribosomes and concurrent recruitment of calreticulin and calmodulin transcripts in gravistimulated maize pulvini

The dynamics of polyribosome abundance were studied in gravistimulated maize (Zea mays) stem pulvini. During the initial 15 min of gravistimulation, the amount of large polyribosomes transiently decreased. The transient decrease in polyribosome levels was accompanied by a transient decrease in polyribosome-associated mRNA. After 30 min of gravistimulation, the levels of polyribosomes and the amount of polyribosome-associated mRNA gradually increased over 24 h up to 3- to 4-fold of the initial value. Within 15 min of gravistimulation, total levels of transcripts coding for calreticulin and calmodulin were elevated 5-fold in maize pulvinus total RNA. Transcripts coding for calreticulin and calmodulin were recruited into polyribosomes within 15 min of gravistimulation. Over 4 h of gravistimulation, a gradual increase in the association of calreticulin and calmodulin transcripts with polyribosomes was seen predominantly in the lower one-half of the maize pulvinus; the association of transcripts for vacuolar invertase with polyribosomes did not change over this period. Our results suggest that within 15 min of gravistimulation, the translation of the majority of transcripts associated with polyribosomes decreased, resembling a general stress response. Recruitment of calreticulin and calmodulin transcripts into polyribosomes occurred predominantly in the lower pulvinus one-half during the first 4 h when the presentation time for gravistimulation in the maize pulvinus is not yet complete.

NASA Discipline Plant Biology↗

Consequence analyses of sabotage-induced radiological releases in high-temperature helium-cooled prismatic microreactors

Here, this study analyzes the radiological dose consequences of sabotage-induced accidents at three high-temperature helium-cooled prismatic microreactors (HTPMs) with thermal power ratings of 1, 10, and 50 MWt. Each HTPM employs uranium oxycarbide tristructural isotropic fuel enriched to 19.75 wt% high-assay low-enriched uranium. Simulations were conducted to estimate reactor core inventory at the point of fuel discharge––when the effective multiplication factor reduced to less than 1––representing peak radionuclide inventory. Postulated sabotage scenarios leading to reactor shutdown were analyzed at two intervals: immediately post-shutdown (0 h) and 3 days after shutdown using the SCALE code for radionuclide inventories and the RASCAL tool for dose consequences. Results show that although HTPMs benefit from inherent safety features and robust fuel design, radiological consequences scale with reactor power because of increased source term inventories. Smaller microreactors exhibited proportionally lower dose consequences. To support the economic and regulatory feasibility of microreactor deployment, this study emphasizes the value of a risk-informed, performance-based approach, as supported by regulations like 10 CFR Parts 100 and 53 in the United States. Microreactor developers should perform site-specific assessments of potential sabotage or low-probability, high-consequence events, especially when considering minimal on-site or full off-site emergency response.

Consequence↗

Rapid discovery and evolution of nanosensors containing fluorogenic amino acids

Binding-activated optical sensors are powerful tools for imaging, diagnostics, and biomolecular sensing. However, biosensor discovery is slow and requires tedious steps in rational design, screening, and characterization. Here we report on a platform that streamlines biosensor discovery and unlocks directed nanosensor evolution through genetically encodable fluorogenic amino acids (FgAAs). Building on the classical knowledge-based semisynthetic approach, we engineer ~15 kDa nanosensors that recognize specific proteins, peptides, and small molecules with up to 100-fold fluorescence increases and subsecond kinetics, allowing real-time and wash-free target sensing and live-cell bioimaging. An optimized genetic code expansion chemistry with FgAAs further enables rapid (~3 h) ribosomal nanosensor discovery via the cell-free translation of hundreds of candidates in parallel and directed nanosensor evolution with improved variant-specific sensitivities (up to ~250-fold) for SARS-CoV-2 antigens. Altogether, this platform could accelerate the discovery of fluorogenic nanosensors and pave the way to modify proteins with other non-standard functionalities for diverse applications.

Biosensors↗

Variable redundancy product codes.

Variable redundancy tensor product codes, discussing encoder modes implementation for adaptive coding and feedback transmission

Sollman, G. H.↗

Midcourse maneuver operations program

Midcourse Maneuver Operations Program /MMOP/ computes the required velocity change to correct a spacecraft trajectory. The program establishes the existence of maneuvers which satisfy spacecraft constraints, explores alternate trajectories in the event that some out-of-tolerance condition forces a change in plans, and codes the maneuvers into commands.

Gordon, H. J.↗

Optimization techniques for FORTRAN 4 (G and H) programs written for the IBM 360 under OS

A fairly complete list is reported of programming techniques which are available to the programmer for optimizing the execution time of production programs written in FORTRAN IV (G and H) for the IBM 360 under OS. After the program has been complied under FORTRAN H, OPT=2, then the process of actually changing code begins. The bulk of execution time of FORTRAN programs can almost always be attributed to a few loops, and primary consideration is given to these.

Dean, J. L.↗

A digital communications system for manned spaceflight applications.

A highly efficient, all-digital communications signal design employing convolutional coding and PN spectrum spreading is described for two-way transmission of voice and data between a manned spacecraft and ground. Variable-slope delta modulation is selected for analog/digital conversion of the voice signal, and a convolutional decoder utilizing the Viterbi decoding algorithm is selected for use at each receiving terminal. A PN spread spectrum technique is implemented to protect against multipath effects and to reduce the energy density (per unit bandwidth) impinging on the earth's surface to a value within the guidelines adopted by international agreement. Performance predictions are presented for transmission via a TDRS (tracking and data relay satellite) system and for direct transmission between the spacecraft and earth. Hardware estimates are provided for a flight-qualified communications system employing the coded digital signal design.

Batson, B. H.↗

VHF command system study

Solutions are provided to specific problems arising in the GSFC VHF-PSK and VHF-FSK Command Systems in support of establishment and maintenance of Data Systems Standards. Signal structures which incorporate transmission on the uplink of a clock along with the PSK or FSK data are considered. Strategies are developed for allocating power between the clock and data, and spectral analyses are performed. Bit error probability and other probabilities pertinent to correct transmission of command messages are calculated. Biphase PCM/PM and PCM/FM are considered as candidate modulation techniques on the telemetry downlink, with application to command verification. Comparative performance of PCM/PM and PSK systems is given special attention, including implementation considerations. Gain in bit error performance due to coding is also considered.

Gee, T. H.↗

Nearest-neighbor doublets in protein-coding regions of MS2 RNA

'Nearest neighbor' base pairs ('doublets') in the protein-coding regions of MS2 RNA have been tabulated with respect to their positions in the first two bases of amino acid codons, in the second two bases, or paired by contact between adjoining codons. Considerable variation is evident between numbers of doublets in each of these three possible positions, but the totals of each of the 16 doublets in the coding regions of the MS2 RNA molecule show much less variation. Compilations of doublets in nucleic acid strands have no predictive value for the amino acid composition of proteins coded by such strands.

Jukes, T. H.↗

The proposed coding standard at GSFC

As part of the continuing effort to introduce standardization of spacecraft and ground equipment in satellite systems, NASA's Goddard Space Flight Center and other NASA facilities have supported the development of a set of standards for the use of error control coding in telemetry subsystems. These standards are intended to ensure compatibility between spacecraft and ground encoding equipment, while allowing sufficient flexibility to meet all anticipated mission requirements. The standards which have been developed to date cover the application of block codes in error detection and error correction modes, as well as short and long constraint length convolutional codes decoded via the Viterbi and sequential decoding algorithms, respectively. Included are detailed specifications of the codes, and their implementation. Current effort is directed toward the development of standards covering channels with burst noise characteristics, channels with feedback, and code concatenation.

Morakis, J. C.↗

A connection between block and convolutional codes

Convolutional codes of any rate and any constraint length give rise to a sequence of quasi-cyclic codes. Conversely, any quasi-cyclic code may be convolutionally encoded. Among the quasi-cyclic codes are the quadratic residue codes, Reed-Solomon codes and optimal BCH codes. The constraint length K for the convolutional encoding of many of these codes (Golay, (48, 24) QR, etc.) turns out to be surprisingly small. Thus using the soft decoding techniques for convolutional decoding, a new maximum likelihood decoding algorithm for many block codes is established. Conversely an optimal quasi-cyclic code will yield a convolutional encoding with optimal local properties and therefore with good infinite convolutional coding properties.

Solomon, G.↗

Calculation of two-dimensional inlet flow fields in a supersonic free stream: Program documentation and test cases

The use of a computer code for the calculation of two dimensional inlet flow fields in a supersonic free stream and a nonorthogonal mesh-generation code are illustrated by specific examples. Input, output, and program operation and use are given and explained for the case of supercritical inlet operation at a subdesign Mach number (M Mach free stream = 2.09) for an isentropic-compression, drooped-cowl inlet. Source listings of the computer codes are also provided.

Biringen, S. H.↗

Polar symmetry in three-dimensional analysis of laminates with angle plies

An improved procedure for the three dimensional finite element analysis of an angle ply laminate with a circular hole is discussed. The procecure exploits polar symmetry and is the basis for a new finite element computer code. For the broad class of laminates that contain angle plies, this code requires only one half as much computing capacity as conventional codes.

Raju, I. S.↗

Coherence bandwidth loss in transionospheric radio propagation

In this report a theoretical model is developed that predicts the single-point, two-frequency coherence function for transionospheric radio waves. The theoretical model is compared to measured complex frequency correlation coefficients using data from the seven equispaced, phase-coherent UHF signals transmitted by the Wideband satellite. The theory and data are in excellent agreement. The theory is critically dependent upon the power-law index, and the frequency coherence data clearly favor the comparatively small spectral indices that have been consistently measured from the wideband satellite phase data. A model for estimating the pulse delay jitter induced by the coherence bandwidth loss is also developed and compared with the actual delay jitter observed on synthesized pulses obtained from the Wideband UFH comb. The results are in good agreement with the theory. The results presented in this report, which are based on an asymptotic theory, are compared with the more commonly used quadratic theory. The model developed and validated in this report can be used to predict the effects of coherence bandwidth loss in disturbed nuclear environments. Simple formulas for the resultant pulse delay jitter are derived that can be used in predictive codes.

Rino, C. L.↗

Experimental and theoretical study of three interacting, closely-spaced, sharp-edged 60 deg delta wings at low speeds

Wind tunnel tests were conducted to determine the subsonic longitudinal aerodynamic characteristics of lifting configuration consisting of a 60 deg delta main wing with two smaller 60 deg delta wings (called sub-wings) attached underneath. The test was designed to determine the effects on lift, drag, and pitching moment due to various placement of the subwings in relation to the main wing. Test results indicate the increasing vertical separation between the main wing and the sub-wings produced the most significant results; a 23.1% increase in maximum lift coefficient, a reduction in drag coefficient at high lift coefficients, and an increase in longitudinal stability. Lateral separation of the sub-wings produced no significant changes. Placement of the sub-wings rearward increases the initial lift curve slope and maximum lift coefficient and also increase the longitudinal stability. Results of a computer study using a vortex lattice code supported the experimental conclusions.

Faery, H. F., Jr.↗

Validation of a three-dimensional viscous analysis of axisymmetric supersonic inlet flow fields

A three-dimensional viscous marching analysis for supersonic inlets was developed. To verify this analysis several benchmark axisymmetric test configurations were studied and are compared to experimental data. Detailed two-dimensional results for shock-boundary layer interactions are presented for flows with and without boundary layer bleed. Three dimensional calculations of a cone at angle of attack and a full inlet at attack are also discussed and evaluated. Results of the calculations demonstrate the code's ability to predict complex flow fields and establish guidelines for future calculations using similar codes.

Benson, T. J.↗