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At least 217 records · Page 12

A synthetic co-culture for bioproduction of ammonia from methane and air

Abstract Fixed nitrogen fertilizers feed 50% of the global population, but most fixed nitrogen production occurs using energy-intensive Haber–Bosch-based chemistry combining nitrogen (N2) from air with gaseous hydrogen (H2) from methane (CH4) at high temperatures and pressures in large-scale facilities sensitive to supply chain disruptions. This work demonstrates the biological transformation of atmospheric N2 into ammonia (NH3) using CH4 as the sole carbon and energy source in a single vessel at ambient pressure and temperature, representing a biological “room-pressure and room-temperature” route to NH3 that could ultimately be developed to support compact, remote, NH3 production facilities amenable to distributed biomanufacturing. The synthetic microbial co-culture of engineered methanotroph Methylomicrobium buryatense (now Methylotuvimicrobium buryatense) and diazotroph Azotobacter vinelandii converted three CH4 molecules to l-lactate (C3H6O3) and powered gaseous N2 conversion to NH3. The design used division of labor and mutualistic metabolism strategies to address the oxygen sensitivity of nitrogenase and maximize CH4 oxidation efficiency. Media pH and salinity were central variables supporting co-cultivation. Carbon concentration heavily influenced NH3 production. Smaller-scale NH3 production near dispersed, abundant, and renewable CH4 sources could reduce disruption risks and capitalize on untapped energy resources. One-Sentence Summary Co-culture of engineered microorganisms Methylomicrobium buryatense and Azotobacter vinelandii facilitated the use of methane gas as a sole carbon feedstock to produce ammonia in an ambient temperature, atmospheric pressure, single-vessel system.

Biotechnology & Applied Microbiology↗

Protecting rain forests and forager's rights using LANDSAT imagery

Creating rain forest reserves is vital given the global decline in biodiversity. Yet, the plants and animals that will be protected from untrammeled commercial exploitation within such reserves constitute essential resources for indigenous foragers and farmers. Balancing the needs of local subsistence level populations with the goals of national and international conservation agencies requires a thorough understanding of the mutual impacts that arise from the interaction of park and people. In the Ituri forest of Zaire, LANDSAT TM image analysis and GPS ground truth data were used to locate human settlements so that boundaries of the proposed Okapi Reserve could be chosen to minimize its impact on the subsistence practices of the local foragers and farmers. Using satellite imagery in conjunction with cultural information should help to ensure traditional resource exploitation rights of indigenous peoples whilst simultaneously protecting the largest contiguous area of undisturbed forest.

Wilkie, David S.↗

Cross-Cultural Barriers to Effective Communication in Aviation

Communication is essential to safe flight, as evidenced by several accidents in which crew communicates was found to have contributed to the accidents. This chapter documents the essential role of explicit efficient communication to flight safety with a global context. It addresses communication between flight crews and air traffic controllers in regions a the world where pilots and controllers speak different native languages, as well as cases in which crew members within the flight deck represent different native languages and cultures. It also addresses problems associated with "exporting" crew resource management training programs to parts of the world which values and norms differ from those of the United States, where these programs were initially developed. This chapter is organized around several central questions: (1) What are various kinds of communication failures and what are their consequences; (2) What are the causes of communication failure; (3) What are features of effective crew communication; (4) What can be done to enhance communication success? To explore a wider range of communication failures than available from accident reports, we examined a set of incident reports from the Aviation Safety Reporting System. These could be classified into three major categories: those in which language actually interfered with transmission of a message; those in which transmission was adequate but the context was not expressed unambiguously and thus the message received was not the same as the message intended; and those in which the message was received as intended, but was not adequately understood or acted upon, mainly because of cultural factors. The consequences of failed communication can be flight errors (such as when a clearance is not received correctly), loss of situation awareness, or failure of crew members (or ATC and pilots) to build a shared understanding of a situation. Causes of misunderstanding can be traced to a number of sources, often grounded in faulty assumptions held by one or both parties to a conversation. Speakers and listeners often experience "illusionary understanding" in which they think they understand each other, but in fact do not. While this problem can exist within a single culture, it is much more serious across cultures. Training in effective explicit communication is a component of Crew Resource Management training programs developed in the U.S. These programs are being adopted by airlines around the world, with varying degrees of success. The level of success in part depends on how similar the conversational and social styles of those cultures are to those of the U.S. A factor that influences conversational style is a culture's relative positioned on two major dimensions that distinguish national cultural groups: individualism vs. collectivism and degree of power distance. The chapter concludes with a discussion of techniques for overcoming the various classes of communication failures and for effectively adapting training programs to fit the values and norms of cultures around the globe.

Fischer, U.↗

Prism: Who, What, How [Slides]

Prism, the Lab’s Lesbian, Gay, Bisexual, Transgender, and Queer+ (LGBTQ+) Employee Resource Group (ERG), fosters an inclusive workplace culture that supports the LGBTQ+ employee base. Prism provides and promotes LGBTQ+ visibility among staff and with management about current workplace and social issues that affect the LGBTQ+ community. This report also details 2022 accomplishments and ongoing initiatives.

99 GENERAL AND MISCELLANEOUS↗

Citizen's actions

The role played by individual citizens as consumers of energy was examined, with emphasis on studying ways in which their action could result in energy conservation. It was shown that there are ways that energy can be conserved in this way, with citizens acting either individually or in groups. The potential savings are significant, but the actual savings may be quite small. The citizens need to be motivated to save and to believe in a conservation ethic; developing such an ethic is difficult, and perhaps not responsive to the shotgun approach now being attempted. The true course of action may be to synthesize new societal structures that provide the maximum evolution of culture within the limitation of scarce energy resources.

Source record↗

Marshall Space Flight Center Propulsion Systems Department (PSD) KM Initiative

NASA Marshall Space Flight Center s Propulsion Systems Department (PSD) is four months into a fifteen month Knowledge Management (KM) initiative to support enhanced engineering decision making and analyses, faster resolution of anomalies (near-term) and effective, efficient knowledge infused engineering processes, reduced knowledge attrition, and reduced anomaly occurrences (long-term). The near-term objective of this initiative is developing a KM Pilot project, within the context of a 3-5 year KM strategy, to introduce and evaluate the use of KM within PSD. An internal NASA/MSFC PSD KM team was established early in project formulation to maintain a practitioner, user-centric focus throughout the conceptual development, planning and deployment of KM technologies and capabilities with in the PSD. The PSD internal team is supported by the University of Alabama's Aging Infrastructure Systems Center Of Excellence (AISCE), Intergraph Corporation, and The Knowledge Institute. The principle product of the initial four month effort has been strategic planning of PSD KM implementation by first determining the "as is" state of KM capabilities and developing, planning and documenting the roadmap to achieve the desired "to be" state. Activities undertaken to support the planning phase have included data gathering; cultural surveys, group work-sessions, interviews, documentation review, and independent research. Assessments and analyses have been performed including industry benchmarking, related local and Agency initiatives, specific tools and techniques used and strategies for leveraging existing resources, people and technology to achieve common KM goals. Key findings captured in the PSD KM Strategic Plan include the system vision, purpose, stakeholders, prioritized strategic objectives mapped to the top ten practitioner needs and analysis of current resource usage. Opportunities identified from research, analyses, cultural/KM surveys and practitioner interviews include: executive and senior management sponsorship, KM awareness, promotion and training, cultural change management, process improvement, leveraging existing resources and new innovative technologies to align with other NASA KM initiatives (convergence: the big picture). To enable results based incremental implementation and future growth of the KM initiative, key performance measures have been identified including stakeholder value, system utility, learning and growth (knowledge capture, sharing, reduced anomaly recurrence), cultural change, process improvement and return-on-investment. The next steps for the initial implementation spiral (focused on SSME Turbomachinery) have been identified, largely based on the organization and compilation of summary level engineering process models, data capture matrices, functional models and conceptual-level systems architecture. Key elements include detailed KM requirements definition, KM technology architecture assessment, evaluation and selection, deployable KM Pilot design, development, implementation and evaluation, and justifying full implementation (estimated Return-on-Investment). Features identified for the notional system architecture include the knowledge presentation layer (and its components), knowledge network layer (and its components), knowledge storage layer (and its components), User Interface and capabilities. This paper provides a snapshot of the progress to date, the near term planning for deploying the KM pilot project and a forward look at results based growth of KM capabilities with-in the MSFC PSD.

Caraccioli, Paul↗

Marshall Space Flight Center Propulsion Systems Department (PSD) Knowledge Management (KM) Initiative

NASA Marshall Space Flight Center's Propulsion Systems Department (PSD) is four months into a fifteen month Knowledge Management (KM) initiative to support enhanced engineering decision making and analyses, faster resolution of anomalies (near-term) and effective, efficient knowledge infused engineering processes, reduced knowledge attrition, and reduced anomaly occurrences (long-term). The near-term objective of this initiative is developing a KM Pilot project, within the context of a 3-5 year KM strategy, to introduce and evaluate the use of KM within PSD. An internal NASA/MSFC PSD KM team was established early in project formulation to maintain a practitioner, user-centric focus throughout the conceptual development, planning and deployment of KM technologies and capabilities within the PSD. The PSD internal team is supported by the University of Alabama's Aging Infrastructure Systems Center of Excellence (AISCE), lntergraph Corporation, and The Knowledge Institute. The principle product of the initial four month effort has been strategic planning of PSD KNI implementation by first determining the "as is" state of KM capabilities and developing, planning and documenting the roadmap to achieve the desired "to be" state. Activities undertaken to suppo~th e planning phase have included data gathering; cultural surveys, group work-sessions, interviews, documentation review, and independent research. Assessments and analyses have beon pedormed including industry benchmarking, related local and Agency initiatives, specific tools and techniques used and strategies for leveraging existing resources, people and technology to achieve common KM goals. Key findings captured in the PSD KM Strategic Plan include the system vision, purpose, stakeholders, prioritized strategic objectives mapped to the top ten practitioner needs and analysis of current resource usage. Opportunities identified from research, analyses, cultural1KM surveys and practitioner interviews include: executive and senior management sponsorship, KM awareness, promotion and training, cultural change management, process improvement, leveraging existing resources and new innovative technologies to align with other NASA KM initiatives (convergence: the big picture). To enable results based incremental implementation and future growth of the KM initiative, key performance measures have been identified including stakeholder value, system utility, learning and growth (knowledge capture, sharing, reduced anomaly recurrence), cultural change, process improvement and return-on-investment. The next steps for the initial implementation spiral (focused on SSME Turbomachinery) have been identified, largely based on the organization and compilation of summary level engineering process models, data capture matrices, functional models and conceptual-level svstems architecture. Key elements include detailed KM requirements definition, KM technology architecture assessment, - evaluation and selection, deployable KM Pilot design, development, implementation and evaluation, and justifying full implementation (estimated Return-on-Investment). Features identified for the notional system architecture include the knowledge presentation layer (and its components), knowledge network layer (and its components), knowledge storage layer (and its components), User Interface and capabilities. This paper provides a snapshot of the progress to date, the near term planning for deploying the KM pilot project and a forward look at results based growth of KM capabilities with-in the MSFC PSD.

Caraccioli, Paul↗

Metabolic engineering of Synechococcus elongatus 7942 for enhanced sucrose biosynthesis

The capability of cyanobacteria to produce sucrose from CO 2 and light has a remarkable societal and biotechnological impact since sucrose can serve as a carbon and energy source for a variety of heterotrophic organisms and can be converted into value-added products. However, most metabolic engineering efforts have focused on understanding local pathway alterations that drive sucrose biosynthesis and secretion in cyanobacteria rather than analyzing the global flux re-routing that occurs following induction of sucrose production by salt stress. Here, we investigated global metabolic flux alterations in a sucrose-secreting (cscB-overexpressing) strain relative to its wild-type Synechococcus elongatus 7942 parental strain. Here we used targeted metabolomics, 13 C metabolic flux analysis (MFA), and genome-scale modeling (GSM) as complementary approaches to elucidate differences in cellular resource allocation by quantifying metabolic profiles of three cyanobacterial cultures - wild-type S. elongatus 7942 without salt stress (WT), wild-type with salt stress (WT/NaCl), and the cscB-overexpressing strain with salt stress (cscB/NaCl) - all under photoautotrophic conditions. We quantified the substantial rewiring of metabolic fluxes in WT/NaCl and cscB/NaCl cultures relative to WT and identified a metabolic bottleneck limiting carbon fixation and sucrose biosynthesis. This bottleneck was subsequently mitigated through heterologous overexpression of glyceraldehyde-3-phosphate dehydrogenase in an engineered sucrose-secreting strain. Our study also demonstrates that combining 13 C-MFA and GSM is a useful strategy to both extend the coverage of MFA beyond central metabolism and to improve the accuracy of flux predictions provided by GSM.

09 BIOMASS FUELS↗

Moving Beyond Bias

The benefits of diverse work groups have been well documented, and our leaders speak of the need for our laboratories to become more diverse and inclusive. Despite these motivators, the field of accelerator controls remains strikingly homogeneous. This trend continues despite many long standing programs to attract underrepresented groups to STEM careers and the explicit desire of leadership to create more inclusive organizations. Research consistently points to the strong role implicit bias plays in preventing organizations from truly providing equal opportunities. The desire to become more diverse must be coupled with a strong culture, cultivated to change deeply rooted practices which influence recruiting, hiring, development, and promotion decisions based on stereotypes rather than accomplishments and abilities. Real change in this arena requires intentional action across the board, not just from human resources and underrepresented groups. This paper discusses practical approaches to changing organizational culture to enable diverse work groups to grow and thrive.

White, Karen S.↗

An overview: recycling nutrients from crop residues for space applications

Without some form of regenerative life support system, long duration space habitation or travel will be limited severely by the prohibitive costs of resupplying air, water, and food from Earth. Components under consideration for inclusion in a regenerative life support system are based on either physicochemical or biological processes. Physicochemical systems would use filtration and elemental phase changes to convert waste materials into usable products, while biological systems would use higher plants and bioreactors to supply crew needs. Neither a purely biological nor strictly a physicochemical approach can supply all crew needs, thus, the best each approach can offer will be combined into a hybrid regenerative life support system. Researchers at Kennedy Space Center (KSC) Advanced Life Support Breadboard Project have taken the lead on bioregenerative aspects of space life support. The major focus has been on utilization of higher plants for production of food, oxygen, and clean water. However, a key to any regenerative life support system is recycling and recovery of resources (wastes). In keeping with the emphasis at KSC on bioregenerative systems and with the focus on plants, this paper focuses on research with biologically-based options for resource recovery from inedible crop residues.

Biomass↗

Origin of biogeographically distinct ecotypes during laboratory evolution

Resource partitioning is central to the incredible productivity of microbial communities, including gigatons in annual methane emissions through syntrophic interactions. Previous work revealed how a sulfate reducer (Desulfovibrio vulgaris, Dv) and a methanogen (Methanococcus maripaludis, Mm) underwent evolutionary diversification in a planktonic context, improving stability, cooperativity, and productivity within 300-1000 generations. Here, we show that mutations in just 15 Dv and 7 Mm genes within a minimal assemblage of this evolved community gave rise to co-existing ecotypes that were spatially enriched within a few days of culturing in a fluidized bed reactor. The spatially segregated communities partitioned resources in the simulated subsurface environment, with greater lactate utilization by attached Dv but partial utilization of resulting H 2 by low affinity hydrogenases of Mm in the same phase. The unutilized H 2 was scavenged by high affinity hydrogenases of planktonic Mm, producing copious amounts of methane. Our findings show how a few mutations can drive resource partitioning amongst niche-differentiated ecotypes, whose interplay synergistically improves productivity of the entire mutualistic community.

59 BASIC BIOLOGICAL SCIENCES↗

CCE Phase 1: Consequence Prioritization

Idaho National Laboratory (INL) developed the Consequence-driven Cyber-informed Engineering (CCE) methodology to provide public and private organizations with steps to work collaboratively and establish a working relationship to protect critical infrastructure and other national assets. This process is a considerable undertaking, iterative in nature, and—as time and resources allow—should become a part of a company’s culture. By focusing on the impact of potentially negative Events, CCE provides a better understanding of how and why adversaries can affect critical functions and services using cyber-enabled sabotage. This document describes Phase 1 of the CCE process.

99 GENERAL AND MISCELLANEOUS↗

NSRC New Hire Orientation: Resources and What to Expect

Hello, New Hire! Right now, you are likely very excited to begin your position at the Laboratory. We are happy you are here! This document will walk you through different facets of your pre-security clearance interval at the Lab. This is a general guide not firm documentation. Its contents were compiled by National Security Research Center (NSRC) team members to make your transition easier. To begin, you will need a security clearance to do your job. A security clearance is essentially an extremely detailed background check. After you complete and turn in your security paperwork, the clearance process can take as little as three months or as long as one year. You may wonder, “If I can’t do my job until after I receive a clearance, what will I be doing?” There is plenty of unclassified work and professional development to be done. Waiting for a clearance can be trying, but we are here to help. This guide details what to expect during pre-clearance, resources to stay industrious, entry points to Lab networking and culture, and tips for success. Patience, flexibility, and productivity are key. Your workday responsibilities and priorities will vary depending on the self-study, assigned duties, and client-driven projects designated by your manager. This guide is here to help, and so are your NSRC colleagues.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗

Meaningful experiences in science education: Engaging the space researcher in a cultural transformation to greater science literacy

The visceral appeal of space science and exploration is a very powerful emotional connection to a very large and diverse collection of people, most of whom have little or no perspective about what it means to do science and engineering. Therein lies the potential of space for a substantially enhanced positive impact on culture through education. This essay suggests that through engaging more of the space research and development community in enabling unique and 'meaningful educational experiences' for educators and students at the pre-collegiate levels, space science and exploration can amplify its positive feedback on society and act as an important medium for cultural transformation to greater science literacy. I discuss the impact of space achievements on people and define what is meant by a 'meaningful educational experience,' all of which points to the need for educators and students to be closer to the practice of real science. I offer descriptions of two nascent science education programs associated with NASA which have the needed characteristics for providing meaningful experiences that can cultivate greater science literacy. Expansion of these efforts and others like it will be needed to have the desired impact on culture, but I suggest that the potential for the needed resources is there in the scientific research communities. A society in which more people appreciate and understand science and science methods would be especially conducive to human progress in space and on Earth.

Morrow, Cherilynn A.↗

Experiences with Testing the Largest Ground System NASA Has Ever Built

In the 1980s, the National Aeronautics and Space Administration (NASA) embarked upon a major Earth-focused program called Mission to Planet Earth. The Goddard Space Flight Center (GSFC) was selected to manage and develop a key component - the Earth Observing System (EOS). The EOS consisted of four major missions designed to monitor the Earth. The missions included 4 spacecraft. Terra (launched December 1999), Aqua (launched May 2002), ICESat (Ice, Cloud, and Land Elevation Satellite, launched January 2003), and Aura (scheduled for launch January 2004). The purpose of these missions was to provide support for NASA s long-term research effort for determining how human-induced and natural changes affect our global environment. The EOS Data and Information System (EOSDIS), a globally distributed, large-scale scientific system, was built to support EOS. Its primary function is to capture, collect, process, and distribute the most voluminous set of remotely sensed scientific data to date estimated to be 350 Gbytes per day. The EOSDIS is composed of a diverse set of elements with functional capabilities that require the implementation of a complex set of computers, high-speed networks, mission-unique equipment, and associated Information Technology (IT) software along with mission-specific software. All missions are constrained by schedule, budget, and staffing resources, and rigorous testing has been shown to be critical to the success of each mission. This paper addresses the challenges associated with the planning, test definition. resource scheduling, execution, and discrepancy reporting involved in the mission readiness testing of a ground system on the scale of EOSDIS. The size and complexity of the mission systems supporting the Aqua flight operations, for example, combined with the limited resources available, prompted the project to challenge the prevailing testing culture. The resulting success of the Aqua Mission Readiness Testing (MRT) program was due in no small measure to re-structuring the traditional programmatic and technical approach to a more efficient and robust program. Programmatically, it meant gaining the endorsement, commitment, and cooperation of the numerous subsystem element managers and other stakeholder organizations. Technically, it required an MRT program that was agile, could rapidly adapt to requirements changes, and was flexible in its overall approach. Furthermore, this paper addresses the following questions: 1. What are the key ingredients (e.g., test tools, organization) needed to conduct a successful MRT program? 2. What distinguishes EOS MRT from the traditional system testing approach? 3. Where should the focus of testing be since it is infeasible to test every element or subsystem? 4. How can MRT be applied effectively to other systems or missions? To provide answers to these questions, this paper relies heavily on real-life, hands-on experiences ("lessons learned") gained during mission readiness testing of the Terra ground system and, most recently, the Aqua and ICESat missions. Moreover, this paper explores how lessons learned were turned into lessons applied for the upcoming Aura mission. Although derived from the EOS missions, MRT techniques and strategies can be applied to enhance the testing of other missions.

Lehtonen, Ken↗

Evaluating Rock Pool Hydroperiod Fluctuation using Climate Variables to Inform Habitat Monitoring and Protection in the Western Sonoran Desert

As warming and drying trends continue to impact the greater American Southwest, effective ecosystem management increasingly relies upon understanding the relationships between climate and water resources. Ephemeral freshwater rock pools, known as tinajas, have great ecological and cultural importance as some of the only sources of surface water in the western Sonoran Desert (WSD). Tinaja flooding and drying cycles, known as hydroperiods, vary based on meteorologic and climatologic conditions; however, a lack of extensive research relating climatic impacts to tinajas puts these critical ecosystems further at risk. Tinajas throughout the WSD are monitored by the National Park Service (NPS) using resource intensive strategies including in situ trail camera observation and direct measurement. To aid NPS monitoring efforts, this research used remotely sensed climate data to analyze spatiotemporal climate trends and relationships between climate variables and tinaja hydroperiods in the WSD between 1979–2022. Using Aqua and Terra Moderate Resolution Imaging Spectroradiometers (MODIS), University of Idaho Gridded Surface Meteorological Dataset (gridMET), and OpenET data, the project analyzed land surface temperature, evapotranspiration, precipitation, wind velocity, and solar radiation. The team generated climate anomaly time series and climate normal maps for the WSD, identifying statistically significant spatial and temporal trends. These data were then compared to daily, qualitative in situ hydroperiod observations taken between 2019–2022. This work will be used by the NPS to inform the monitoring and protection of tinajas in the WSD. Findings contribute to a limited body of research concerning climate and tinajas, which are often overlooked despite their disproportionate ecological importance.

Annie Britton↗

Applications of satellite image processing to the analysis of Amazonian cultural ecology

This paper examines the application of satellite image processing towards identifying and comparing resource exploitation among indigenous Amazonian peoples. The use of statistical and heuristic procedures for developing land cover/land use classifications from Thematic Mapper satellite imagery will be discussed along with actual results from studies of relatively small (100 - 200 people) settlements. Preliminary research indicates that analysis of satellite imagery holds great potential for measuring agricultural intensification, comparing rates of tropical deforestation, and detecting changes in resource utilization patterns over time.

Behrens, Clifford A.↗

Integrated risk management

The purpose of this report is to first present a basis or foundation for the building of an integrated risk management plan and them to present the plan. The integration referred to is across both the temporal and the hierarchical dimensions. Complexity, consequence, and credibility seem to be driving the need for the consideration of risk. Reduction of personal bias and reproducibility of the decision making process seem to be driving the consideration of a formal risk plan. While risk can be used as either a selection tool or a control tool, this paper concentrates on the selection usage. Risk relies on stated purpose. The tightness of the definition of purpose and success is directly reflected in the definition and control of risk. Much of a risk management plan could be designed by the answers to the questions of why, what, who, when, and where. However, any plan must provide the following information about a threat or risk: likelihood, consequence, predictability, reliability, and reproducibility. While the environment at NASA is seen as warm, but not hot, for the introduction of a risk program, some encouragement is seen if the following problems are addressed: no champion, no commitment of resource, confused definitions, lack of direction and focus, a hard sell, NASA culture, many choices of assessment methods, and cost. The plan is designed to follow the normal method of doing work and is structured to follow either the work break down structure or a functional structure very well. The parts of the plan include: defining purpose and success, initial threat assessment, initial risk assessment, reconciling threats and parameters, putting part of the information down and factoring the information back into the decision process as it comes back up, and developing inferences. Two major suggestions are presented. One is to build an office of risk management to be used as a resource by managers in doing the risk process. Another is to form a pilot program to try out the details in the plan and modify the method where needed.

Hunsucker, J. L.↗