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At least 217 records · Page 12

Phosphoproteomics profiling of human skin fibroblast cells reveals pathways and proteins affected by low doses of ionizing radiation

Background: High doses of ionizing radiation result in biological damage, however the precise relationships between long term health effects, including cancer, and low dose exposures remain poorly understood and are currently extrapolated using high dose exposure data. Identifying the signaling pathways and individual proteins affected at the post-translational level by radiation should shed valuable insight into the molecular mechanisms that regulate dose dependent responses to radiation. Principle Findings: We have identified 6845 unique phosphopeptides (2566 phosphoproteins) from control and irradiated (2 and 50 cGy) primary human skin fibroblasts one hour post-exposure. Dual statistical analyses based on spectral counts and peak intensities identified 287 phosphopeptides (from 231 proteins) and 244 phosphopeptides (from 182 proteins) that varied significantly following exposure to 2 and 50 cGy respectively. This screen identified phosphorylation sites on proteins with known roles in radiation responses including TP53BP1 as well as previously unidentified radiation responsive proteins such as the candidate tumor suppressor SASH1. Bioinformatics analyses suggest that low and high doses of radiation affect both overlapping and unique biological processes and suggest a role of MAP kinase and protein kinase A (PKA) signaling in the radiation response as well as differential regulation of p53 networks at low and high doses of radiation. Conlcusions: Our results represent the most comprehensive analysis of the phosphoproteomes of human primary fibroblasts exposed to multiple doses of ionizing radiation published to date and provides a basis for the systems level identification of biological processes, molecular pathways and individual proteins regulated in a dose dependent manner by ionizing radiation. Further study of these modified proteins and affected networks should help to define the molecular mechanisms that regulate biological responses to radiation at different radiation doses and elucidate the impact of low dose radiation exposure on human health.

59 BASIC BIOLOGICAL SCIENCES↗

Background and radiation resistance tests of neutral particle analyzer detectors for ITER by using a fast neutron beam

The radiation resistance and background sensitivity of scintillation (Hamamatsu H8500D photo-multiplier) and semiconductor (ORTEC BF-018-100-60 and BU-012-050-100) detectors to neutron and gamma radiation were investigated. Conclusions are drawn concerning the possibility of using such detectors in neutral particle analyzers that are being developed for ITER at the Ioffe Institute.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Development of a New Analysis Tool for Evaluating and Correcting for Weather Conditions that Constrain Radiation Portal Monitor Performance

Researchers at Oak Ridge National Laboratory (ORNL) developed the Adaptable, Multiplatform, Real-Time Analysis Package (AMRAP) for the continuous measurement of environmental radionuclide decay. AMRAP is a completely open source visualization and analysis package capable of combining a variety of data streams into an array of real-time plots. Once acquired, data streams are analyzed to store static images and extract data based on previously defined thresholds. AMRAP is currently used at ORNL to combine data streams from an Ortec Detective high-purity germanium (HPGe) detector, a TSA Systems radiation portal monitor (RPM), and an Orion weather station. The combined data are used to study the rain-induced increase in RPM background radiation levels. RPMs experience an increase in background radiation during precipitation due to the deposition of atmospheric radionuclides on the ground. Using AMRAP results in a real-time analysis workstation specifically dedicated to the study of RPM background radiation levels. By means of an editable library of common inputs, AMRAP is adaptable to remote monitoring applications that would benefit from the real-time visualization and analysis of radiation measurements. To study rain-induced increases in background radiation levels observed in radiation portal monitors (RPMs), researchers at Oak Ridge National Laboratory (ORNL) developed a software package that allows data with different formats to be analyzed and plotted in near real time. The Adaptable, Multiplatform, Real-Time Analysis Package (AMRAP) was developed to operate in the background and capture plots of important data based on previously defined thresholds. After executing AMRAP, segments of a data stream can be captured without additional post-processing. AMRAP can also display previously recorded data to facilitate a detailed offline analysis. Without access to these capabilities in a single software package, analyzing multiple continuously recorded data streams with different formats is impractical. Commercially available acquisition software packages record and analyze radiation measurements but are not designed to perform real-time analysis in conjunction with data from other vendors. The lack of collaboration between vendors is problematic when research requires different data streams to be correlated in time and immediately analyzed. AMRAP was specifically developed to provide a solution to this problem. AMRAP is a completely open source visualization and analysis package capable of plotting and analyzing data from different vendors in near real time.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Initial Modeling of Urban Search Measurements

Detection of illicit nuclear materials in urban environments is difficult due to the large amount of background radiation from naturally occurring radioactive materials (NORM) in the roadways and buildings. Mobile searches suffer from low count rates, so the detection algorithms must be carefully balanced between missing real sources and reporting too many false alarms. The Modeling Urban Scenarios and Experiments (MUSE) project aims to create a virtual testbed for simulation of radiation detection to predict realistic background and threat source detection events so that detection algorithms can be better optimized to find illicit sources. The MUSE project is a collaboration of Oak Ridge National Laboratory (ORNL), Lawrence Berkeley National Laboratory (LBNL), and the Remote Sensing Laboratory (RSL). The project also works with staff at Lawrence Livermore National Laboratory (LLNL) to support the development of the Optimization Planning Tool for Urban Search (OPTUS) software package. The first step in creating a virtual testbed is to determine the level of detail required in the models to match actual measurements. Several measurement campaigns were conducted in 2015 and 2016 at the Fort Indiantown Gap (FTIG) Combined Arms Collective Traming Facility (CACTF). This facility contains a representative urban environment, with a dozen buildings and several streets. Detectors commonly used in search operations - 2 in x 4 in x 16 in NaI(Tl) - were used to measure count rates from background and sources. Measurements with a shielded high-purity germanium detector of the roadways, sidewalks, and building walls were made to determine the concentration of NORM that makes up the bulk of the background radiation. This paper shows some comparisons of modeling simulations to measurements taken during the OPTUS-3 campaign in November 2015. Measurements with and without an 81 {mu}Ci cesium source were made at several points along the center line of the main road. Initial results show good agreement between the measured and simulated spectra above 300 keV. Additional studies to determine the causes of mismatch below 300 keV have been started and will also be discussed.

07 ISOTOPE AND RADIATION SOURCES↗

Calibration facility for environment dosimetry instruments

In the last ten years, the nuclear activities, as well as the major nuclear events (see Fukushima accident) had an increasing impact on the environment, merely by contamination with radioactive materials. The most conferment way to quickly identify the presence of some radioactive elements in the environment, is to measure the dose-equivalent rate H. In this situation, information concerning the values of H due only to the natural radiation background must exist. Usually, the values of H due to the natural radiation background, are very low (∼10{sup −9} - 10{sup −8} Sv/h). A correct measurement of H in this range involve a performing calibration of the measuring instruments in the measuring range corresponding to the natural radiation background lead to important problems due to the presence of the natural background itself the best way to overlap this difficulty is to set up the calibration stand in an area with very low natural radiation background. In Romania, we identified an area with such special conditions at 200 m dept, in a salt mine. This paper deals with the necessary requirements for such a calibration facility, as well as with the calibration stand itself. The paper includes also, a description of the calibration stand (and images) as well as the radiological and metrological parameters. This calibration facilities for environment dosimetry is one of the few laboratories in this field in Europe.

61 RADIATION PROTECTION AND DOSIMETRY↗

Estimating radiological background using imaging spectroscopy

Optical imaging spectroscopy is investigated as a method to estimate radiological background by spectral identification of soils, sediments, rocks, minerals and building materials derived from natural materials and assigning tabulated radiological emission values to these materials. Radiological airborne surveys are undertaken by local, state and federal agencies to identify the presence of radiological materials out of regulatory compliance. Detection performance in such surveys is determined by (among other factors) the uncertainty in the radiation background; increased knowledge of the expected radiation background will improve the ability to detect low-activity radiological materials. Radiological background due to naturally occurring radiological materials (NORM) can be estimated by reference to previous survey results, use of global 40K, 238U, and 232Th (KUT) values, reference to existing USGS radiation background maps, or by a moving average of the data as it is acquired. Each of these methods has its drawbacks: previous survey results may not include recent changes, the global average provides only a zero-order estimate, the USGS background radiation map resolutions are coarse and are accurate only to 1 km – 25 km sampling intervals depending on locale, and a moving average may essentially low pass filter the data to obscure small changes in radiation counts. Imaging spectroscopy from airborne or spaceborne platforms can offer higher resolution identification of materials and background, as well as provide imaging context information. AVIRIS hyperspectral image data is analyzed using commercial exploitation software to determine the usefulness of imaging spectroscopy to identify qualitative radiological background emissions when compared to airborne radiological survey data.

background radiation estimation↗

New detector for use in fast neutron radiography

We have developed and tested a new type detector for use in the fast neutron (FN) imaging radiography applications. FN radiography is generally used for nondestructive material testing, medical and biology applications, border patrol, transportation and cargo screening tasks. It is complementary to other types of radiography, providing additional information on light element content of the material samples. Distinct from other FN imagers presently known, our device implements a neutron-sensitive scintillator attached to a position-sensitive photomultiplier tube (PSPMT), and operates in an event-by-event readout mode, acquiring energy, timing, and pulse shape information for all detected radiation events. The information is used to help separate events of FN interactions in the scintillator from the background events caused by the electronics noise and by the other types of background radiation. Selection of pure fast neutron events in the final image allows us to achieve ultimate image contrast and resolution, as compared with other types of FN imaging devices operating most commonly in an integration mode, in which the detector?s dark noise and radiation background dilute the images. The detector performance for FN imaging application was tested using D-D neutron generator, designed and manufactured by Adelphi Technology, Inc. This essentially point-like neutron source operates in continuous mode producing up to 109 of 2.5 MeV neutrons per second. Samples made of metals plastic and other material were used to measure the detector resolution, efficiency and uniformity. Results of these tests are presented and discussed. Fig. 1 shows one of the test FN radiographic images obtained using the sample made of 11 styrene plastic strips. All strips are squares 4.8 x 4.8 mm2 with six different lengths 10 to 60 mm with 10 mm increment. [A] [B] [C] Fig. 1. [A]-layout of the test sample; [B]-raw FN shadow image of the sample; [C]-map of the plastic strips as they appear on the image, with measured FN absorption factor shown in percent. Both X and Y position resolution of the FN imaging detector are estimated to be less than 0.5 mm (sigma) based on the analysis of the edges of the strip shadows in the plot. Because this detector shows the fraction-of-a-millimeter resolution desirable for most of FN applications, and is built using radiation hard materials, we believe it could be a good alternative to other FN imaging systems based on CCD or solid state detectors. In addition, having a sub-nanosecond timing resolution, it is suitable for the time-of-flight energy-resolved FN imaging.

Popov, V.↗

Astronomy in the region 1-0.1mm wavelengths

Measurements in five different passbands in the spectral region below 20/cm were made with a liquid helium-cooled radiometer in two flights at approximately 40 km altitude. The equivalent temperatures are given for passbands of 1 to 5.4, 7.8, 7.9, 11.1, and 18.5/cm. The results are consistent with a 2.7 K thermal radiation background. Atmospheric radiation of uncertain magnitude dominates the region above 11/cm.

Weiss, R.↗

The formation of massive primordial stars in the presence of moderate UV backgrounds

Radiative feedback produced by stellar populations played a vital role in early structure formation. In particular, photons below the Lyman limit can escape the star-forming regions and produce a background ultraviolet (UV) flux, which consequently may influence the pristine halos far away from the radiation sources. These photons can quench the formation of molecular hydrogen by photodetachment of H{sup –}. In this study, we explore the impact of such UV radiation on fragmentation in massive primordial halos of a few times 10{sup 7} M {sub ☉}. To accomplish this goal, we perform high resolution cosmological simulations for two distinct halos and vary the strength of the impinging background UV field in units of J {sub 21} assuming a blackbody radiation spectrum with a characteristic temperature of T {sub rad} = 10{sup 4} K. We further make use of sink particles to follow the evolution for 10,000 yr after reaching the maximum refinement level. No vigorous fragmentation is observed in UV-illuminated halos while the accretion rate changes according to the thermal properties. Our findings show that a few 10{sup 2}-10{sup 4} solar mass protostars are formed when halos are irradiated by J {sub 21} = 10-500 at z > 10 and suggest a strong relation between the strength of the UV flux and mass of a protostar. This mode of star formation is quite different from minihalos, as higher accretion rates of about 0.01-0.1 M {sub ☉} yr{sup –1} are observed by the end of our simulations. The resulting massive stars are potential cradles for the formation of intermediate-mass black holes at earlier cosmic times and contribute to the formation of a global X-ray background.

79 ASTRONOMY AND ASTROPHYSICS↗

On the source function of the soft X-ray diffuse background

Radiation transfer theory has been used recently to derive the source function of the soft X-ray diffuse background, resulting in the claim of evidence for 10 exp 6 K gas in the Galactic halo. We show that this analysis has several errors that invalidate its conclusions. We argue that the case for an extensive hot halo remains open, pending further work, but may be settled by the continuing series of Rosat observations of high-latitude soft X-ray shadows.

Burrows, David N.↗

Infrared observations of contaminants from Shuttle flight 51-F

A small helium-cooled infrared telescope, IRT, was flown on the Shuttle in July/August 1985. The principal astrophysical objectives were to measure the large scale structure of sources and the background radiation. A cold shutter was incorporated to permit absolute flux measurements. Additionally, the engineering objectives included setting upper limits on the infrared radiation from the local environment. Even though the local background overwhelmed the astrophysical background, astronomical sources were still detectable superimposed on this background radiation. Data are presented covering the spectral range from 2 microns to 120 microns. The spatial, spectral, and temporal variations are described. Based on the spectral character and variability in different wavelength bands, the background radiation does not appear to have a single origin. In this paper, the results on the Shuttle environment are presented.

Koch, D. G.↗

Evaluating the Impact of a Canadian National Anatomy and Radiology Contouring Boot Camp for Radiation Oncology Residents

Background: Radiation therapy treatment planning has advanced over the past 2 decades, with increased emphasis on 3-dimensional imaging for target and organ-at-risk (OAR) delineation. Recent studies suggest a need for improved resident instruction in this area. We developed and evaluated an intensive national educational course (“boot camp”) designed to provide dedicated instruction in site-specific anatomy, radiology, and contouring using a multidisciplinary (MDT) approach. Methods: The anatomy and radiology contouring (ARC) boot camp was modeled after prior single-institution pilot studies and a needs-assessment survey. The boot camp incorporated joint lectures from radiation oncologists, anatomists, radiologists, and surgeons, with hands-on contouring instruction and small group interactive seminars using cadaveric prosections and correlative axial radiographs. Outcomes were evaluated using pretesting and posttesting, including anatomy/radiology multiple-choice questions (MCQ), timed contouring sessions (evaluated relative to a gold standard using Dice similarity metrics), and qualitative questions on satisfaction and perceived effectiveness. Analyses of pretest versus posttest scores were performed using nonparametric paired testing. Results: Twenty-nine radiation oncology residents from 10 Canadian universities participated. As part of their current training, 29%, 75%, and 21% receive anatomy, radiology, and contouring instruction, respectively. On posttest scores, the MCQ knowledge scores improved significantly (pretest mean 60% vs posttest mean 80%, P<.001). Across all contoured structures, there was a 0.20 median improvement in students' average Dice score (P<.001). For individual structures, significant Dice improvements occurred in 10 structures. Residents self-reported an improved ability to contour OARs and interpret radiographs in all anatomic sites, 92% of students found the MDT format effective for their learning, and 93% found the boot camp more effective than educational sessions at their own institutions. All of the residents (100%) would recommend this course to others. Conclusions: The ARC boot camp is an effective intervention for improving radiation oncology residents' knowledge and understanding of anatomy and radiology in addition to enhancing their confidence and accuracy in contouring.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Energy spectrum of extragalactic gamma-ray sources

The result of Monte Carlo electron photon cascade calculations for propagation of gamma rays through regions of extragalactic space containing no magnetic field are given. These calculations then provide upper limits to the expected flux from extragalactic sources. Since gamma rays in the 10 to the 14th power eV to 10 to the 17th power eV energy range are of interest, interactions of electrons and photons with the 3 K microwave background radiation are considered. To obtain an upper limit to the expected gamma ray flux from sources, the intergalactic field is assumed to be so low that it can be ignored. Interactions with photons of the near-infrared background radiation are not considered here although these will have important implications for gamma rays below 10 to the 14th power eV if the near infrared background radiation is universal. Interaction lengths of electrons and photons in the microwave background radiation at a temperature of 2.96 K were calculated and are given.

Protheroe, R. J.↗

CARBON IONIZATION STATES AND THE COSMIC FAR-UV BACKGROUND WITH He II ABSORPTION

We constrain the spectrum of the cosmic ultraviolet background radiation by fitting the observed abundance ratios of carbon ions at z {approx} 2-3 with those expected from different models of the background radiation. We use the recently calculated modulation of the background radiation between 3 and 4 Ryd due to resonant line absorption by intergalactic He II and determine the ratios of C III to C IV expected at these redshifts, as functions of metallicity, gas density, and temperature. Our analysis of the observed ratios shows that 'delayed reionization' models, which assume a large fraction of He II at z {approx} 3, are not favored by data. Our results suggest that He II reionization was inhomogeneous, consistent with the predictions from recent simulations.

79 ASTRONOMY AND ASTROPHYSICS↗

Management of Sentinel Lymph Node Metastasis in Merkel Cell Carcinoma: Completion Lymphadenectomy, Radiation, or Both?

Background: Approximately 30% of patients with clinically localized Merkel cell carcinoma (MCC) show nodal involvement on sentinel lymph node biopsy (SLNB). Optimal management of SLNB-positive disease has not been defined. This study compared outcomes after completion lymphadenectomy (CLND), radiation, and combined CLND plus radiation after a positive SLNB. Methods: All patients treated at a single institution for SLNB-positive MCC (1998–2015) were retrospectively evaluated, with examination of patient demographics, clinicopathologic characteristics, outcomes, and regional toxicity. Results: The study identified 71 evaluable patients with SLNB-positive disease. The median age of these patients was 76 years, and 76.1% were men. Of the 71 patients, 11 (15.5%) underwent CLND, 40 (56.3%) received radiation, and 20 (28.2%) underwent CLND plus postoperative radiation. Lymphovascular invasion was significantly more common in the radiation-alone cohort (p = 0.04). For the three cohorts, the median percentages of nodal involvement were respectively 2, 10, and 30% (p = 0.06). After a median follow-up period of 22.3 months, four patients had recurrence in their regional nodal basin (3 radiation-alone patients and 1 CLND + radiation patient). The three cohorts did not differ significantly in the development of distant metastases (p = 0.68) or overall survival (p = 0.72). Six patients experienced surgical-site infections (2 CLND and 4 CLND + radiation patients), and three patients experienced symptomatic lymphedema (1 CLND patient and 2 CLND + radiation patients). Conclusions: Regional failure was infrequent (≤ 10%) regardless of treatment, and morbidity appeared to be low with all approaches. Given that multiple treatment approaches can be successful in treating micrometastatic MCC, future efforts should be directed at refining criteria for allocating patients to a specific method, or possibly no further nodal basin treatment, in an effort to maximize regional control at the lowest cost and morbidity.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Predicting Future Solar Modulation and Implementation in MCNP6

The application of detecting low level radiation in the field requires quantification of the natural radiation background specific to that location. This background is made up two primary components: naturally decaying radionuclides in the soil and atmosphere known as terrestrial background, and the particle cascades caused by high energy cosmic radiation interacting within the earth's atmosphere known as cosmic background. The terrestrial component dominates the background spectrum below approximately 2 MeV, however, the cosmic component comprises the entirety of the high-energy spectrum. Furthermore, the terrestrial component is relatively constant for a given location while the cosmic component is heavily dependent on solar activity, an effect known as solar modulation. Annual values of the solar modulation parameter, φ, have been determined since 1936, and their fluctuations are seen to be roughly sinusoidal over time. If one wished to predict or simulate the outcome of an experiment in the future, a prediction for the solar modulation would be necessary to accurately determine the cosmic background contribution. The previous version 6.1.1 of MCNP contained solar modulation data for 46 years between 1960 and 2005. This data was updated with newer solar modulation data, from I. G. Usoskin et al., which reports solar modulation data for an additional 33 years between 1936 and 2014. In order to use past solar modulation data to predict cosmic background radiation fluctuations for dates beyond 2014 an algorithm was developed to forecast future solar modulation data based on the sinusoidal behavior exhibited in recorded data. This algorithm was implemented into MCNP 6.1.2 to be used for cosmic background simulations and tested against benchmark data to assess its accuracy. (authors)

54 ENVIRONMENTAL SCIENCES↗

Proposal of a Nomogram for Predicting Survival in Patients with Siewert Type II Adenocarcinoma of the Esophagogastric Junction After Preoperative Radiation

Background: Preoperative radiotherapy tends to be more frequently used for patients with adenocarcinoma of the esophagogastric junction (AEG); however, the prognostic values of postoperative pathologic characteristics in these patients remain unclear. This study aimed to examine the outcomes in Siewert type II AEG patients receiving preoperative radiotherapy to identify the predictive factors for overall survival (OS). Methods and Results: A total of 1818 AEG patients undergoing preoperative radiotherapy were reviewed. Univariate analyses showed that age, sex, histology, tumor grade, positive lymph node (PLN), lymph node ratio, and log odds of positive lymph nodes (LODDS) were significantly correlated with OS; however, only age, grade, PLN, and LODDS were identified as independent risk factors in a multivariate regression model. Subsequently, patients were randomly grouped into training and validation cohorts (1:1 ratio), and the beta coefficients of these variables in the training set were used to generate the nomogram. The composite nomogram showed improved prognostic accuracy in the training, validation, and entire cohorts compared with that of TNM stage alone. Conclusions: In conclusion, our proposed nomogram represents a promising tool for estimating OS in Siewert type II AEG patients after preoperative radiotherapy.

62 RADIOLOGY AND NUCLEAR MEDICINE↗