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At least 199 records · Page 11

Thermal loading on self-seeding monochromators in x-ray free electron lasers

One approach to achieve longitudinally coherent hard X-ray free electron laser (XFEL) radiation is to introduce a single-crystal monochromator in the beam path to generate a co-propagating, time-synchronized self-seeding radiation pulse. For modern high-repetition rate XFELs, however, thermal loading on the crystal can degrade the quality of the seed pulse. To assess limitations on the maximum XFEL pulse repetition rate imposed by the seeding crystal, a comprehensive study of thermomechanical, and X-ray diffraction effects within the crystal is presented in this paper for two consecutive self-amplified spontaneous emission pulses incident on both reflective and transmissive crystal monochromators.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Molecular dynamics simulation of seeded crystal growth in glass

The mechanism of non-congruent growth of a crystal from glass has been sought using molecular dynamics simulations. Specifically, as a model of this process, the growth of a lithium niobate (LiNbO 3 ) crystal seed sandwiched between two lithium niobosilicate (LNS) glass slabs has been simulated as a function of time and temperature. The growth of pre-existing crystal is strongly affected by the orientation of crystal seed, temperature, and the SiO 2 concentration in the surrounding LNS glass matrix. The orientation of LiNbO 3 seed surface that has inherently larger interplanar distance results in a relatively slower crystal growth. The addition of SiO 2 to LNS system significantly decreases the crystal growth, which primarily occurs in the region devoid of Si. The suppressive effect of SiO 2 on growth rate can be traced to the existence of defect complex comprising of Si substituted at the Nb site and a nearby Nb vacancy.

36 MATERIALS SCIENCE↗

Long-term, large-scale experiment reveals the effects of seed limitation, climate, and anthropogenic disturbance on restoration of plant communities in a biodiversity hotspot

Ecological restoration is essential for maintaining biodiversity in the face of dynamic, global changes in climate, human land use, and disturbance regimes. Effective restoration requires understanding bottlenecks in plant community recovery that exist today, while recognizing that these bottlenecks may relate to complex histories of environmental change. Such understanding has been a challenge because few long-term, well-replicated experiments exist to decipher the demographic processes influencing recovery for numerous species against the backdrop of multiyear variation in climate and management. Here we address this challenge through a long-term and geographically expansive experiment in longleaf pine savannas, an imperiled ecosystem and biodiversity hotspot in the southeastern United States. Using 48 sites at three locations spanning 480 km, the 8-y experiment manipulated initial seed arrival for 24 herbaceous plant species and presence of competitors to evaluate the impacts of climate variability and management actions (e.g., prescribed burning) on plant establishment and persistence. Adding seeds increased plant establishment of many species. Cool and wet climatic conditions, low tree density, and reduced litter depth also promoted establishment. Once established, most species persisted for the duration of the 8-y experiment. Plant traits were most predictive when tightly coupled to the process of establishment. Our results illustrate how seed additions can restore plant diversity and how interannual climatic variation affects the dynamics of plant communities across a large region. The significant effects of temperature and precipitation inform how future climate may affect restoration and conservation via large-scale changes in the fundamental processes of establishment and persistence.

60 APPLIED LIFE SCIENCES↗

Radiative divertor detachment and impurity transport with nitrogen and neon seeding in KSTAR H-mode plasmas

Achieving core-edge compatible divertor detachment is a critical requirement for stable operation in future fusion devices. This study compares nitrogen and neon seeding in KSTAR H-mode plasmas with carbon walls, combining experiments and SOLPS-ITER modelling to evaluate their radiative dissipation and core-edge compatibility. Experimentally, N seeding achieved stronger divertor detachment, with larger reductions in target particle and heat fluxes, a higher divertor radiation fraction, and a lower core radiation fraction than Ne. In contrast, Ne seeding triggered a significant rise in core radiation followed by H–L back transitions, limiting the maximum total radiated power fraction to roughly half that of N. SOLPS-ITER simulations reproduced the experimental trends and revealed that the better core-edge compatibility of N arises from its higher divertor retention in addition to its higher cooling factor. The relative positions of the stagnation points of impurity poloidal velocity and ionization sources did not explain the different divertor compression. Instead, in the present modelling, the higher impurity parallel particle flux, resulting from the higher parallel impurity velocity, explains the stronger nitrogen impurity compression in the divertor region. The parallel temperature distribution with N was more favourable for achieving higher impurity parallel velocity than with Ne, because the impurity velocity is governed by modifications of the main ion flow due to friction and thermal forces, both of which strongly depend on the temperature. Ultimately, this behaviour is attributed to the strongly divertor-localized radiation of N. These results demonstrate that N is more effective than Ne in achieving radiative divertor detachment while maintaining low core contamination in KSTAR, consistent with observations in other present tokamaks.

KSTAR↗

Dynamic pulse-to-pulse thermal load effects in pulse-train-mode self-seeded X-ray free-electron laser

Thermal load has been a haunting factor that undermines the brightness and coherence of the self-seeded X-ray free-electron laser. Additionally, different from uniformly pulsed mode, in pulse train mode a thermal quasi-steady state of the crystal monochromator may not be reached. This leads to a dynamic thermal distortion of the spectral transmission curves and seed quality degradation. In this paper, the pulse-to-pulse thermal load effects on the spectral transmission curves and seed quality are shown, and some instructive information for the tuning process is provided.

47 OTHER INSTRUMENTATION↗

Two-stage reflective self-seeding scheme for high-repetition-rate X-ray free-electron lasers

X-ray free-electron lasers (XFELs) open a new era of X-ray based research by generating extremely intense X-ray flashes. To further improve the spectrum brightness, a self-seeding FEL scheme has been developed and demonstrated experimentally. As the next step, new-generation FELs with high repetition rates are being designed, built and commissioned around the world. A high repetition rate would significantly speed up the scientific research; however, alongside this improvement comes new challenges surrounding thermal management of the self-seeding monochromator. Here, a new configuration for self-seeding FELs is proposed, operated under a high repetition rate which can strongly suppress the thermal effects on the monochromator and provides a narrow-bandwidth FEL pulse. Three-dimension time-dependent simulations have been performed to demonstrate this idea. Finally, with this proposed configuration, high-repetition-rate XFEL facilities are able to generate narrow-bandwidth X-ray pulses without obvious thermal concern on the monochromators.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

MYC transcription factors coordinate tryptophan–dependent defence responses and compromise seed yield in Arabidopsis

Robust plant immunity negatively affects other fitness traits, including growth and seed production. Jasmonate (JA) confers broad-spectrum protection against plant consumers by stimulating the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins, which in turn relieves repression on transcription factors (TFs) coincident with reduced growth and fecundity. The molecular mechanisms underlying JA-mediated decreases in fitness remain largely unknown. To assess the contribution of MYC TFs to growth and reproductive fitness at high levels of defence, we mutated three MYC genes in a JAZ-deficient mutant (jazD) of Arabidopsis thaliana that exhibits strong defence and low seed yield. Genetic epistasis analysis showed that de-repression of MYC TFs in jazD not only conferred strong resistance to insect herbivory but also reduced shoot and root growth, fruit size and seed yield. We also provided evidence that the JAZ–MYC module coordinates the supply of tryptophan with the production of indole glucosinolates and the proliferation of endoplasmic reticulum bodies that metabolise glucosinolates through the action of b-glucosidases. Our results establish MYCs as major regulators of growth- and reproductive–defence tradeoffs and further indicate that these factors coordinate tryptophan availability with the production of amino acid-derived defence compounds.

59 BASIC BIOLOGICAL SCIENCES↗

Characterization of Kariya ( Hildegardia barteri (Mast.) Kosterm) Seed Oil Fatty Acid Methyl Ester Prepared from Basic Catalytic Transesterification

The rising global energy demand, alongside concerns regarding environmental deterioration due to the use of fossil fuels, has spurred extensive investigation into renewable energy alternatives. Biomass-derived biodiesel, especially from lesser-known oil sources, emerges as a promising option. This research focuses on analyzing the fatty acid methyl esters (FAMEs) derived from Kariya (Hildegardia barteri (Mast.) Kosterm) seed oil through basic catalytic transesterification using gas chromatography–flame ionization detector (GC–FID) analysis, assessing its potential as a biodiesel feedstock. Oil extraction from Kariya seeds was carried out using three solvents (n-hexane, ethanol, and a 1:1 blend of hexane and ethanol), followed by transesterification with methanol. Gas chromatography–mass spectrometry (GC–MS) and GC–FID analyses were utilized to identify and quantify FAMEs in the resulting biodiesel. The results revealed various FAMEs, including methyl myristate, methyl palmitate, methyl stearate, methyl oleate, methyl linoleate, and methyl linolenate. Significant differences in FAME composition were observed among the samples, with hexane–ethanol Kariya oil biodiesel (HE-KOB) showing the highest FAME content (76.1%). This combination of solvents exhibited synergistic effects on the composition of HE-KOB, suggesting potential optimization strategies for biodiesel production. Fourier transform infrared spectroscopy (FTIR) provided additional insights into the molecular composition of the biodiesel samples, confirming their biodiesel nature through the identified functional groups such as methyl, methylene, hydrocarbon, ester, aldehyde, and alkene. Thermogravimetric analysis (TGA) for thermal decomposition also gave an insight into FAME composition and its contribution to the degree of conversion of biodiesel to energy. These findings highlight the feasibility of utilizing Kariya seed oil as a biodiesel feedstock, emphasizing the importance of solvent selection and transesterification conditions in optimizing FAME yield and composition. This research contributes to the exploration of underutilized oil sources for sustainable biodiesel production, aligning with the global shift towards cleaner and renewable energy sources.

09 BIOMASS FUELS↗

Assessing the use of UAS-related terms in ASRS using Seed Topic Modeling

Context: The NASA Aviation Safety Reporting System (ASRS) is a voluntary confidential aviation safety reporting system. The ASRS receives reports from pilots, air traffic controllers, flight attendants and other involved in aviation operations. The reports are de-identified and coded by ASRS expert safety analysts. The de-identified reports are then disseminated to the aviation community in a number of ways including entry into an online database. Augmenting the discovery of topics of user interest in this online database would therefore be beneficial to the community it serves. Aim: We propose and execute an experiment to assess the use of seed term topic modeling using the database narratives to identify UAS reports. The use of seed term topic modeling would enable users to identify groups of related narratives associated to a topic of their interest. Method: We use a newly curated field in ASRS reports which identify UAS from non-UAS reports in combination of different set of UAS related terms to assess if seed topic modeling can be used in ASRS.

LDA↗

Seeding Advanced Treated Wastewater for Purposes of Direct Potable Reuse

Direct potable reuse (DPR) is a promising solution to address water scarcity. However, a better understanding of how introducing advanced treated water (ATW) affects microbial communities present in distribution systems is needed. Here, in this study, we measured changes to the microbial water quality in simulated distribution systems that were conditioned using treated, unimpaired surface water (SW) and then transitioned to ATW. In addition, we investigated whether adding a biological filtration step would seed the microbial community of the ATW and whether the influence would persist in the simulated distribution systems. We found that the bulk water in the ATW-fed distribution systems had lower cell counts and ATP concentrations and a distinct microbial community (based on 16S amplicon sequencing) compared to the SW-fed or the seeded ATW-fed systems. However, biofilm community composition and biomass remained consistent regardless of the feedwater. Increased microbial biomass and diversity were present in the seeded ATW, with several amplicon sequence variants identified as being introduced by the biological filter. Our results suggest that directly introducing ATW to distribution systems could disturb the existing microbial community. Preparing ATW for distribution via biological filtration may deliver more predictable and stable microbial water quality than introducing unseeded ATW.

16S↗

Revisiting the Effects of Seed Liquid Water Content on Isoprene Secondary Organic Aerosol Growth

Aerosol liquid water content (LWC) has been shown to enhance partitioning of water-soluble oxygenated organic vapors into the aerosol particles and facilitate aqueous chemistry, impact SOA formation, composition, and physicochemical properties. Here, this study examines the effect of aerosol liquid water content (LWC) on isoprene SOA formation using effloresced and deliquesced ammonium sulfate ((NH 4 ) 2 SO 4 ) and sodium chloride (NaCl) seed particles in an environmental chamber with photo-oxidation extending over hours under moderate relative humidity (50%-60%). Our findings indicate that isoprene SOA mass yield is not significantly influenced (p value >> 0.05) by the phase state or the chemical identity of the seed particles within a 3–4-hour reaction time scale. Detailed molecular analysis using advanced high resolution mass spectrometry indicates that while both effloresced and deliquesced seed types produced similar molecular formula distributions, deliquesced (NH 4 ) 2 SO 4 slightly favored the production of less volatile compounds. Depth-profiling analysis by single particle mass spectrometry confirmed that isoprene SOA predominantly located on the surfaces of particles with core-shell morphologies, with partial dissolution of the effloresced inorganic salt observed even below the deliquescence relative humidity (DRH) of salt. This finding suggests that assuming dry salt core particles do not interact with SOA coatings, based on salt deliquescence points, may overlook important aqueous chemistry. These results emphasize the critical role of reaction time scale and mixing state on SOA formation and call for further research to understand LWC effects on SOA from different precursors.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Twin-pulse seeding enables pump-probe capabilities in the EUV to soft X-ray spectrum at synchrotron light sources

Having previously reported that separating the two stages of echo-enabled harmonic generation (EEHG) with one or more bending magnet (BM) sections allows the BMs to serve as the desired source of momentum compaction, here we demonstrate that this arrangement can greatly reduce the total energy modulation required by any 4th generation synchrotron light source, leading to higher repetition rates as well as stronger coherent radiation output power, with significant benefits. Since the EEHG beamline performance is mainly determined by the momentum compaction, beam emittances and beta functions of a storage ring lattice, allowing for different separations between the two stages is a straightforward way to increase the momentum compaction of chicane 1. This also enables pump-probe capabilities in a novel context, where twin-pulse seeding on the same electron bunch would allow two distinct radiation pulses with an adjustable delay in the range of 0.1 to 10 ps. In the twin-pulse seeding scheme, the same electron bunch could undergo modulation from two distinct laser pulses. Later stages would produce independent harmonics in subsequent straight sections. There are two variations of this twin-pulse seeding scheme, supporting different scientific applications. With a common modulation in stage 1, the first option allows simultaneously two independent radiation sources, with a full coverage of the EUV (2.5 to 50 nm) to soft X-ray (1.25 to 2.5 nm) spectrum; for the second option, the same stage 2 undulator could generate two coherent pulses both fitting within the FEL bandwidth, or at distinct harmonics. We present particle tracking simulation studies based on the APS-U lattice, including quantum excitation and radiation damping. These simulations indicate that there is no degradation of the modulated longitudinal phase space even when the two stages are separated by as many as 10 BM sections.

36 MATERIALS SCIENCE↗

Improved core-edge compatibility using impurity seeding in the small angle slot (SAS) divertor at DIII-D

Impurity seeding studies in the slot divertor (SAS) at DIII-D have revealed a strong relationship between the detachment onset and pedestal characteristics with both target geometry and impurity species. N 2 seeding in the slot has led to the first simultaneous observation of detachment on the entire suite of boundary diagnostics viewing the SAS without degradation of core confinement. SOLPS-ITER simulations with D+C+N, full cross-field drifts and n-n collisions activated are performed for the first time in DIII-D to interpret the behavior. This illustrates a strong effect of divertor configuration and plasma drifts on the recycling source distribution with significant consequences on plasma flows. Flow reversal is found for both main ions and impurities affecting strongly the impurity transport and providing an explanation for the observed dependence on strike point location of the detachment onset and impurity leakage found in the experiments. Matched discharges with either nitrogen or neon injection show that while nitrogen does not significantly affect the pedestal, neon leads to increased pedestal pressure gradient and improved pedestal stability. Little nitrogen penetrates in the core, but a great amount of neon is found in the pedestal consistent with the different ionization potentials of the two impurities. This research demonstrates that neutral and impurity distributions in the divertor can be controlled through variations in strike point locations in a fixed baffle structure. Divertor geometry combined with impurity seeding enables mitigated divertor heat flux balancing core contamination and thus leading to enhanced divertor dissipation and improved core-edge compatibility which are essential for ITER and for future fusion reactors.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A physics-informed deep learning model of the hot tail runaway electron seed

A challenging aspect of the description of a tokamak disruption is evaluating the hot tail runaway electron seed that emerges during the thermal quench. This problem is made challenging due to the requirement of describing a strongly non-thermal electron distribution, together with the need to incorporate a diverse range of multiphysics processes, including magnetohydrodynamic instabilities, impurity transport, and radiative losses. Here this work develops a physics-informed neural network (PINN) tailored to the solution of the hot tail seed during an axisymmetric thermal quench. Here, a PINN is developed to identify solutions to the adjoint relativistic Fokker–Planck equation in the presence of a rapid quench of the plasma's thermal energy. It is shown that the PINN is able to accurately predict the hot tail seed across a range of parameters, including the thermal quench timescale, initial plasma temperature, and local current density, in the absence of experimental or simulation data. The hot tail PINN is verified by comparison with direct Monte Carlo simulations, with excellent agreement found across a broad range of thermal quench conditions.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Structure-based design of nanobodies that inhibit seeding of Alzheimer’s patient–extracted tau fibrils

Despite much effort, antibody therapies for Alzheimer’s disease (AD) have shown limited efficacy. Challenges to the rational design of effective antibodies include the difficulty of achieving specific affinity to critical targets, poor expression, and antibody aggregation caused by buried charges and unstructured loops. To overcome these challenges, we grafted previously determined sequences of fibril-capping amyloid inhibitors onto a camel heavy chain antibody scaffold. These sequences were designed to cap fibrils of tau, known to form the neurofibrillary tangles of AD, thereby preventing fibril elongation. The nanobodies grafted with capping inhibitors blocked tau aggregation in biosensor cells seeded with postmortem brain extracts from AD and progressive supranuclear palsy (PSP) patients. The tau capping nanobody inhibitors also blocked seeding by recombinant tau oligomers. Another challenge to the design of effective antibodies is their poor blood–brain barrier (BBB) penetration. In this study, we also designed a bispecific nanobody composed of a nanobody that targets a receptor on the BBB and a tau capping nanobody inhibitor, conjoined by a flexible linker. We provide evidence that the bispecific nanobody improved BBB penetration over the tau capping inhibitor alone after intravenous administration in mice. Our results suggest that the design of synthetic antibodies that target sequences that drive protein aggregation may be a promising approach to inhibit the prion-like seeding of tau and other proteins involved in AD and related proteinopathies.

60 APPLIED LIFE SCIENCES↗

The achievement of the T e,div feedback control by CD 4 seeding on EAST

A multi-function divertor feedback control system has been built on Experimental Advanced Superconducting Tokamak (EAST) to treat the divertor heat load issue. With the real-time data of the Langmuir probes and the impurity seeding, the divertor electron temperature (T e,div ) is well controlled to achieve the partial detachment phase. The first trial of the CD 4 seeding for the T e,div reduction has been achieved on EAST long-pulse discharge. In the seeding phase, the T e,div was maintained close to 5 eV, and the surface temperature of the target plate (T surface,div ) had a reduction of about 150° C. The plasma stored energy had a reduction in the control phase, so it is necessary to find a way to keep the good plasma confinement in the next step. The CD 4 injection also mitigated the low hybrid wave coupling rate in some degree. The big volume of the CD 4 injection lifted the Greenwald density fraction from ~0.4 to ~0.7, which made the SOL into high recycling state. As a result, most of the injected carbon particles were in the high ionized state, and with the lower of the T e,div , the tungsten line emission was suppressed obviously.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Targeted seed EMS mutagenesis reveals a basic helix–loop–helix transcription factor underlying male sterility in sorghum

Abstract Forward genetic screens of mutant populations are fundamental for functional genomics studies. However, isolating independent mutant alleles to molecularly identify causal genes is challenging in species recalcitrant to genetic manipulation. Here, we demonstrate that classic seed ethyl methanesulfonate (EMS) mutagenesis coupled with genome sequencing can overcome this limitation in sorghum. We used this method to generate new mutant alleles of sorghum MALE STERILE 8 (MS8) and identified the causal locus for the ms8 phenotype as Sobic.004G270900, which encodes the sorghum ortholog of maize bhlh122, a basic helix–loop–helix (bHLH) transcription factor required for male fertility in maize. Bulked segregant analysis mapped ms8-1 to a region on chromosome 4 containing Sobic.004G270900. Seeds from heterozygous MS8/ms8-1 plants were mutagenized and screened for chimeric inflorescences containing sectors with white, sterile anthers resembling the ms8-1 homozygous phenotype. DNA sequencing of sterile and fertile sectors from a single chimeric inflorescence revealed two mutations in Sobic.004G270900 within the sterile sector, but not the fertile sector. Isolation of this loss-of-function allele (ms8-2) established Sobic.004G270900 as the causative locus for male sterility in the ms8 mutant. We generated additional alleles of MS8 in a different genetic background using CRISPR/Cas9-based gene editing, where deletions in Sobic.004G270900 also resulted in male sterility. Our work identified a gene underlying male sterility in sorghum and provides a novel and straightforward genetic tool for researchers who lack access to advanced transformation facilities to validate gene candidates. Unlike gene editing, no prior knowledge of candidate genes is required for targeted seed EMS mutagenesis to aid identification of causal loci.

Genetics & Heredity↗

Massive black holes seeded by dark matter: Implications for little red dots and gravitational wave signatures

Observations of supermassive black holes (SMBHs) at high redshifts challenge standard seeding scenarios. We examine a dissipative self-interacting dark matter (dSIDM) model in which gravothermal collapse leads to the formation of massive BH seeds ab initio. We utilize a semianalytical framework to predict properties of the dSIDM-seeded SMBH population. Billion solar mass quasars are reproduced along with low-mass faint active galactic nuclei (known as little red dots) with SMBH-to-galaxy stellar mass ratios consistent with recent James Webb Space Telescope observations. To match the abundance of the observed bright quasars, a subpercent-level duty cycle is suggested, implying a large population of dormant SMBHs. The gravitational wave (GW) signals from mergers of these massive SMBHs can be detected by LISA while remaining within the NANOGrav constraints on the GW background. These results provide testable signatures of DM-driven SMBH formation, offering a pathway to probe hidden-sector physics through SMBH and GW observables.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗