Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “linked open data”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 199 records · Page 11

Joint emulation of Earth System Model temperature-precipitation realizations with internal variability and space-time and cross-variable correlation: fldgen v2.0 software description

Earth System Models (ESMs) are excellent tools for quantifying many aspects of future climate dynamics but are too computationally expensive to produce large collections of scenarios for downstream users of ESM data. In particular, many researchers focused on the impacts of climate change require large collections of ESM runs to rigorously study the impacts to both human and natural systems of low-frequency high-importance events, such as multi-year droughts. Climate model emulators provide an effective mechanism for filling this gap, reproducing many aspects of ESMs rapidly but with lower precision. The fldgen v1.0 R package quickly generates thousands of realizations of gridded temperature fields by randomizing the residuals of pattern scaling temperature output from any single ESM, retaining the spatial and temporal variance and covariance structures of the input data at a low computational cost. The fldgen v2.0 R package described here extends this capability to produce joint realizations of multiple variables, with a focus on temperature and precipitation in an open source software package available for community use (https://github.com/jgcri/fldgen). This substantially improves the fldgen package by removing the requirement that the ESM variables be normally distributed, and will enable researchers to quickly generate covarying temperature and precipitation data that are synthetic but faithful to the characteristics of the original ESM.

54 ENVIRONMENTAL SCIENCES↗

GeneLab: A Systems Biology Platform for Spaceflight Omics Data

NASA's mission includes expanding our understanding of biological systems to improve life on Earth and to enable long-duration human exploration of space. Resources to support large numbers of spaceflight investigations are limited. NASA's GeneLab project is maximizing the science output from these experiments by: (1) developing a unique public bioinformatics database that includes space bioscience relevant "omics" data (genomics, transcriptomics, proteomics, and metabolomics) and experimental metadata; (2) partnering with NASA-funded flight experiments through bio-sample sharing or sample augmentation to expedite omics data input to the GeneLab database; and (3) developing community-driven reference flight experiments. The first database, GeneLab Data System Version 1.0, went online in April 2015. V1.0 contains numerous flight datasets and has search and download capabilities. Version 2.0 will be released in 2016 and will link to analytic tools. In 2015 Genelab partnered with two Biological Research in Canisters experiments (BBRIC-19 and BRIC-20) which examine responses of Arabidopsis thaliana to spaceflight. GeneLab also partnered with Rodent Research-1 (RR1), the maiden flight to test the newly developed rodent habitat. GeneLab developed protocols for maxiumum yield of RNA, DNA and protein from precious RR-1 tissues harvested and preserved during the SpaceX-4 mission, as well as from tissues from mice that were frozen intact during spaceflight and later dissected. GeneLab is establishing partnerships with at least three planned flights for 2016. Organism-specific nationwide Science Definition Teams (SDTs) will define future GeneLab dedicated missions and ensure the broader scientific impact of the GeneLab missions. GeneLab ensures prompt release and open access to all high-throughput omics data from spaceflight and ground-based simulations of microgravity and radiation. Overall, GeneLab will facilitate the generation and query of parallel multi-omics data, and deep curation of metadata for integrative analysis, allowing researchers to uncover cellular networks as observed in systems biology platforms. Consequently, the scientific community will have access to a more complete picture of functional and regulatory networks responsive to the spaceflight environment.. Analysis of GeneLab data will contribute fundamental knowledge of how the space environment affects biological systems, and enable emerging terrestrial benefits resulting from mitigation strategies to prevent effects observed during exposure to space. As a result, open access to the data will foster new hypothesis-driven research for future spaceflight studies spanning basic science to translational science.

proteomics↗

Spatio-temporal dynamics of evapotranspiration on the Tibetan Plateau from 2000 to 2010

Evapotranspiration (ET) is a key process of the climate system because it links water, energy and carbon cycles. In this study we modified a Penman–Monteith based algorithm to estimate ET on the Tibetan Plateau at a 1 km spatial resolution for the period 2000–2010 using meteorological and satellite remote sensing data. The results showed that the average annual ET on the Tibetan Plateau was 350.3 mm year–1 and decreased from the southeast toward the northwest. The highest ET value was found in open water bodies (680.9 mm year–1) and the lowest ET value was found in open shrubland (254.0 mm year–1). Overall, the inter-annual ET decreased from 2000 to 2010 and there was significant negative ET trend over 42% of the region, primarily in the northwest of the Tibetan Plateau. Relative humidity was the dominant factor in controlling long-term variations of ET in the arid northwest plateau. But under moist conditions, leaf area index or temperature drove ET. In addition, P -ET on the Tibetan Plateau significantly increased and about 37% of the region showed strong positive P -ET trend primarily in the central of the Tibetan Plateau. In conclusion, the positive P -ET trend in four seasons suggested that the Tibetan Plateau might have become wetter during the past decade.

58 GEOSCIENCES↗

Status of the Fiber Optic Control System Integration (FOCSI) program

This report presents a discussion of the progress made in the NASA/NAVY Fiber Optic Control System Integration (FOCSI) program. This program will culminate in open-loop flight tests of passive optical sensors and associated electro-optics on an F-18 aircraft. Currently, the program is in the final stages of hardware fabrication and environmental testing of the passive optical sensors and electro-optics. This program is a foundation for future Fly-by-Light (FBL) programs. The term Fly-by-Light is used to describe the utilization of passive optical sensors and fiber optic data links for monitoring and control of aircraft in which sensor and actuation signals are transmitted optically. The benefits of this technology for advanced aircraft include the following: improved reliability and reduced certification cost due to greater immunity to EME (electromagnetic effects); reduced harness volume and weight; elimination of short circuits and sparking in wiring due to insulation deterioration; lower maintenance costs (fewer components); greater flexibility in data bus protocol and architecture; absence of ground loops; and higher operating temperatures for electrically passive optical sensors.

Baumbick, Robert J.↗

Resolving the Discrepancies Between Empirical and Rayleigh Charge Limiting Models for Globular Proteins

Starting with the Rayleigh charge limiting model, a slightly different approach is used to account for the well-known discrepancy that exists between the said model and experimental ESI MS data for globular proteins. It is shown using published datasets that for globular proteins, the mass density ρ exhibits a weak second-order dependence on its mass M, according to ρ(M)∝M{sup -α}, α ~ 0.14. A direct equivalence established between ESI MS and x-ray techniques suggests a minimum but critical surface tension of 15.6 ± 5.2 mN/m for the droplet at the liquid-to-gas phase transition point. The packing density factor η for globular proteins is believed to lie between 1 (very tightly packed) and 4.6 (less tight, natively packed). While the Rayleigh charge limiting model has been linked historically to the CRM (J. Chem. Phys. 49:2240–2249, 1968; Anal. Chim. Acta 406:93–104, 2000), this paper does not expressly seek to justify the CRM, but rather uses empirical data and existing knowledge across subfields to help build a consistent picture of ESI MS phenomena that might be difficult to explain otherwise. These results would be useful in molecular dynamics (MD) simulations, understanding liquid-to-gas phase transitions and in opening up new routes for cross-calibration between ESI MS, IM MS, NMR and x-ray crystallography studies. .

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Practical constraints on network architecture and signalling in the MSAT system

Telesat Mobile Inc. plans to provide mobile satellite communications services in Canada in 1993/4, in close cooperation with the American Mobile Satellite Consortium Inc., which will be providing services in the U.S.A. L-band frequencies will be used in multiple beams for communication with mobile terminals. Ku-band frequencies will be used for the feeder-links to fixed stations. The system will support voice and data communications. The baseline Canadian system will support approximately 450 assignable voice channels, some fraction of which will be the equivalent in data channels. The method of multiple access will be Frequency Division Multiple Access/Single Channel Per Carrier. The availability of frequencies, the availability of technology and the time scale for implementation all constrain the network architecture for the system. Further, it is important to have an open specification to encourage multiple equipment vendors. The interplay of these constraints is discussed.

Davies, N. George↗

A digital seismogram archive of nuclear explosion signals, recorded at the Borovoye Geophysical Observatory, Kazakhstan, from 1966 to 1996

Seismologists from Kazakhstan, Russia, and the United States have rescued the Soviet-era archive of nuclear explosion seismograms recorded at Borovoye in northern Kazakhstan during the period 1966–1996. The signals had been stored on about 8000 magnetic tapes, which were held at the recording observatory. After hundreds of man-years of work, these digital waveforms together with significant metadata are now available via the project URL, namely http://www.ldeo.columbia.edu/res/pi/Monitoring/Data/ as a modern open database, of use to diverse communities. Three different sets of recording systems were operated at Borovoye, each using several different seismometers and different gain levels. For some explosions, more than twenty different channels of data are available. A first data release, in 2001, contained numerous glitches and lacked many instrument responses, but could still be used for measuring accurate arrival times and for comparison of the strengths of different types of seismic waves. The project URL also links to our second major data release, for nuclear explosions in Eurasia recorded in Borovoye, in which the data have been deglitched, all instrument responses have been included, and recording systems are described in detail. This second dataset consists of more than 3700 waveforms (digital seismograms) from almost 500 nuclear explosions in Eurasia, many of them recorded at regional distances. It is important as a training set for the development and evaluation of seismological methods of discriminating between earthquakes and underground explosions, and can be used for assessment of three-dimensional models of the Earth’s interior structure.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Tracking materials science data lineage to manage millions of materials experiments and analyses

Abstract In an era of rapid advancement of algorithms that extract knowledge from data, data and metadata management are increasingly critical to research success. In materials science, there are few examples of experimental databases that contain many different types of information, and compared with other disciplines, the database sizes are relatively small. Underlying these issues are the challenges in managing and linking data across disparate synthesis and characterization experiments, which we address with the development of a lightweight data management framework that is generally applicable for experimental science and beyond. Five years of managing experiments with this system has yielded the Materials Experiment and Analysis Database (MEAD) that contains raw data and metadata from millions of materials synthesis and characterization experiments, as well as the analysis and distillation of that data into property and performance metrics via software in an accompanying open source repository. The unprecedented quantity and diversity of experimental data are searchable by experiment and analysis attributes generated by both researchers and data processing software. The search web interface allows users to visualize their search results and download zipped packages of data with full annotations of their lineage. The enormity of the data provides substantial challenges and opportunities for incorporating data science in the physical sciences, and MEAD’s data and algorithm management framework will foster increased incorporation of automation and autonomous discovery in materials and chemistry research.

Soedarmadji, Edwin↗

Structural insights into plasmalemma vesicle-associated protein (PLVAP): Implications for vascular endothelial diaphragms and fenestrae

In many organs, small openings across capillary endothelial cells (ECs) allow the diffusion of low–molecular weight compounds and small proteins between the blood and tissue spaces. These openings contain a diaphragm composed of radially arranged fibers, and current evidence suggests that a single-span type II transmembrane protein, plasmalemma vesicle-associated protein-1 (PLVAP), constitutes these fibers. Here, we present the three-dimensional crystal structure of an 89-amino acid segment of the PLVAP extracellular domain (ECD) and show that it adopts a parallel dimeric alpha-helical coiled-coil configuration with five interchain disulfide bonds. The structure was solved using single-wavelength anomalous diffraction from sulfur-containing residues (sulfur SAD) to generate phase information. Biochemical and circular dichroism (CD) experiments show that a second PLVAP ECD segment also has a parallel dimeric alpha-helical configuration—presumably a coiled coil—held together with interchain disulfide bonds. Overall, ~2/3 of the ~390 amino acids within the PLVAP ECD adopt a helical configuration, as determined by CD. We also determined the sequence and epitope of MECA-32, an anti-PLVAP antibody. Taken together, these data lend strong support to the model of capillary diaphragms formulated by Tse and Stan in which approximately ten PLVAP dimers are arranged within each 60- to 80-nm-diameter opening like the spokes of a bicycle wheel. Passage of molecules through the wedge-shaped pores is presumably determined both by the length of PLVAP—i.e., the long dimension of the pore—and by the chemical properties of amino acid side chains and N-linked glycans on the solvent-accessible faces of PLVAP.

59 BASIC BIOLOGICAL SCIENCES↗

Investigating the interconnectedness of active transportation and public transit usage as a primer for Mobility-as-a-Service adoption and deployment

With the advent of Mobility-as-a-Service packages to reduce car usage and (by extension) greenhouse gas emissions, it is crucial that researchers and practitioners consider mutual determinants and outcomes that link the adoption of multiple alternative modes. This study therefore investigates the joint usage of active travel (walking, cycling, bike-sharing) and public transit (bus, rail) modes with respect to personal and situational contexts. Online survey data (n = 826) were collected across six Midwestern U.S. states using Amazon MTurk. Respondents indicated their average weekly usage of eight travel modes across three trip purposes so that multimodality could be assessed. Several psychological constructs were extracted via (a) the stages of change framework, used to indicate willingness to adopt new behavior, and (b) confirmatory factor analysis conducted on Likert scales rooted in theories of community, identity, norms, personality, and well-being. A multiple-indicators multiple-causes structural equation model is then employed to investigate the process of adopting a modality style that incorporates active and transit modes. The model confirms that compatible physical and social contexts, as well as navigational skills and openness to learning, are key primers of multimodalism. However, a path juncture stemming from neighborhood support for mobility innovation illustrates a potential polarity in outcomes between individuals and communities. In addition, the stage of active mobility adoption is linked to identity and norm activation, offering further guidance on what influences readiness for change. The seamless integration of mobility services is critical to matching the convenience and comfort of the private vehicle; understanding potential pathways to sustainable mobility, though, requires analyses and interventions that are driven by well-being outcomes and grounded in rigorous psychological frameworks. Here, the research findings offer practical guidance for identifying intervention opportunities to be linked with MaaS enrollment while demonstrating the need to illuminate how mobility might relate to social cohesion, identity expression, and various sources of satisfaction.

42 ENGINEERING↗

Assessing complexity and dynamics in epidemics: geographical barriers and facilitators of foot-and-mouth disease dissemination

Introduction: Physical and non-physical processes that occur in nature may influence biological processes, such as dissemination of infectious diseases. However, such processes may be hard to detect when they are complex systems. Because complexity is a dynamic and non-linear interaction among numerous elements and structural levels in which specific effects are not necessarily linked to any one specific element, cause-effect connections are rarely or poorly observed. Methods: To test this hypothesis, the complex and dynamic properties of geo-biological data were explored with high-resolution epidemiological data collected in the 2001 Uruguayan foot-and-mouth disease (FMD) epizootic that mainly affected cattle. County-level data on cases, farm density, road density, river density, and the ratio of road (or river) length/county perimeter were analyzed with an open-ended procedure that identified geographical clustering in the first 11 epidemic weeks. Two questions were asked: (i) do geo-referenced epidemiologic data display complex properties? and (ii) can such properties facilitate or prevent disease dissemination? Results: Emergent patterns were detected when complex data structures were analyzed, which were not observed when variables were assessed individually. Complex properties–including data circularity–were demonstrated. The emergent patterns helped identify 11 counties as ‘disseminators’ or ‘facilitators’ (F) and 264 counties as ‘barriers’ (B) of epidemic spread. In the early epidemic phase, F and B counties differed in terms of road density and FMD case density. Focusing on non-biological, geographical data, a second analysis indicated that complex relationships may identify B-like counties even before epidemics occur. Discussion: Geographical barriers and/or promoters of disease dispersal may precede the introduction of emerging pathogens. If corroborated, the analysis of geo-referenced complexity may support anticipatory epidemiological policies.

60 APPLIED LIFE SCIENCES↗

Carbon Storage Site Mapping Inquiry Tool (MapIT)

To date, 48 projects, consisting of 139 wells, are currently under review with the Environmental Protection Agency’s (EPA) Underground Injection Control (UIC) Program for Class VI – wells used for geologic sequestration of carbon dioxide. The number of applications submitted is expected to increase in coming years with the increase of the 45Q tax credit available to projects that initiate construction prior to 2033. The amount of data collected to submit a Class VI permit is vast, and often disparate, coming from state, federal, and commercial entities, as well as field-specific data collected within an area of interest. When preparing for site selection and permitting, the initial aggregation of relevant public data can be time intensive. The Carbon Storage Site Mapping Inquiry tool (MapIT) was created to support and accelerate the discovery and accessibility of open-source data and information available across the USA. Data was aggregated and organized based on data types described within the EPA UIC Class VI permit documentation. The online tool enables users to explore hundreds of geospatial data layers and connect to additional external resources, leveraging API and REST services where possible to ensure updates to data in real time. MapIT enables users to explore state and federal data related to geologic, geophysical, structural, hydrologic, and contextual information. In addition to displaying spatial data and linking to external resources, MapIT leverages custom widgets to ensure that internal data and external data are discoverable and accessible. The widgets connect users to resources such as the USGS publications and the USGS Earthquake Catalog based on a user-defined location. This talk will describe data aggregation workflows, data types, data preparation, and tool development for MapIT. The Carbon Storage Site Mapping Inquiry Tool and underlying database are valuable, intuitive resources that empower government, academic, commercial and industry stakeholders to explore, analyze, and acquire carbon storage related data.

Morkner, Paige↗

The emergence of hydrogeophysics for improved understanding of subsurface processes over multiple scales

Geophysics provides a multidimensional suite of investigative methods that are transforming our ability to see into the very fabric of the subsurface environment, and monitor the dynamics of its fluids and the biogeochemical reactions that occur within it. Here we document how geophysical methods have emerged as valuable tools for investigating shallow subsurface processes over the past two decades and offer a vision for future developments relevant to hydrology and also ecosystem science. The field of “hydrogeophysics” arose in the late 1990s, prompted, in part, by the wealth of studies on stochastic subsurface hydrology that argued for better field-based investigative techniques. These new hydrogeophysical approaches benefited from the emergence of practical and robust data inversion techniques, in many cases with a view to quantify shallow subsurface heterogeneity and the associated dynamics of subsurface fluids. Furthermore, the need for quantitative characterization stimulated a wealth of new investigations into petrophysical relationships that link hydrologically relevant properties to measurable geophysical parameters. Development of time-lapse approaches provided a new suite of tools for hydrological investigation, enhanced further with the realization that some geophysical properties may be sensitive to biogeochemical transformations in the subsurface environment, thus opening up the new field of “biogeophysics.” Early hydrogeophysical studies often concentrated on relatively small “plot-scale” experiments. More recently, however, the translation to larger-scale characterization has been the focus of a number of studies. In conclusion, geophysical technologies continue to develop, driven, in part, by the increasing need to understand and quantify key processes controlling sustainable water resources and ecosystem services.

58 GEOSCIENCES↗

AENET–LAMMPS and AENET–TINKER : Interfaces for accurate and efficient molecular dynamics simulations with machine learning potentials

Machine-learning potentials (MLPs) trained on data from quantum-mechanics based first-principles methods can approach the accuracy of the reference method at a fraction of the computational cost. To facilitate efficient MLP-based molecular dynamics and Monte Carlo simulations, an integration of the MLPs with sampling software is needed. Here, we develop two interfaces that link the atomic energy network (ænet) MLP package with the popular sampling packages TINKER and LAMMPS. The three packages, ænet, TINKER, and LAMMPS, are free and open-source software that enable, in combination, accurate simulations of large and complex systems with low computational cost that scales linearly with the number of atoms. Scaling tests show that the parallel efficiency of the ænet–TINKER interface is nearly optimal but is limited to shared-memory systems. The ænet–LAMMPS interface achieves excellent parallel efficiency on highly parallel distributed memory systems and benefits from the highly optimized neighbor list implemented in LAMMPS. We demonstrate the utility of the two MLP interfaces for two relevant example applications: the investigation of diffusion phenomena in liquid water and the equilibration of nanostructured amorphous battery materials.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Closed Loop Geothermal Working Group: GeoCLUSTER App, Subsurface Simulation Results, and Publications

To better understand the heat production, electricity generation performance, and economic viability of closed-loop geothermal systems in hot-dry rock, the Closed-Loop Geothermal Working Group -- a consortium of several national labs and academic institutions has tabulated time-dependent numerical solutions and levelized cost results of two popular closed-loop heat exchanger designs (u-tube and co-axial). The heat exchanger designs were evaluated for two working fluids (water and supercritical CO2) while varying seven continuous independent parameters of interest (mass flow rate, vertical depth, horizontal extent, borehole diameter, formation gradient, formation conductivity, and injection temperature). The corresponding numerical solutions (approximately 1.2 million per heat exchanger design) are stored as multi-dimensional HDF5 datasets and can be queried at off-grid points using multi-dimensional linear interpolation. A Python script was developed to query this database and estimate time-dependent electricity generation using an organic Rankine cycle (for water) or direct turbine expansion cycle (for CO2) and perform a cost assessment. This document aims to give an overview of the HDF5 database file and highlights how to read, visualize, and query quantities of interest (e.g., levelized cost of electricity, levelized cost of heat) using the accompanying Python scripts. Details regarding the capital, operation, and maintenance and levelized cost calculation using the techno-economic analysis script are provided. This data submission will contain results from the Closed Loop Geothermal Working Group study that are within the public domain, including publications, simulation results, databases, and computer codes. GeoCLUSTER is a Python-based web application created using Dash, an open-source framework built on top of Flask that streamlines the building of data dashboards. GeoCLUSTER provides users with a collection of interactive methods for streamlining the exploration and visualization of an HDF5 dataset. The GeoCluster app and database are contained in the compressed file geocluster_vx.zip, where the "x" refers to the version number. For example, geocluster_v1.zip is Version 1 of the app. This zip file also contains installation instructions. **To use the GeoCLUSTER app in the cloud, click the link to "GeoCLUSTER on AWS" in the Resources section below. To use the GeoCLUSTER app locally, download the geocluster_vx.zip to your computer and uncompress this file. When uncompressed this file comprises two directories and the geocluster_installation.pdf file. The geo-data app contains the HDF5 database in condensed format, and the GeoCLUSTER directory contains the GeoCLUSTER app in the subdirectory dash_app, as app.py. The geocluster_installation.pdf file provides instructions on installing Python, the needed Python modules, and then executing the app.

15 GEOTHERMAL ENERGY↗

NASA GeneLab Concept of Operations

NASA's GeneLab aims to greatly increase the number of scientists that are using data from space biology investigations on board ISS, emphasizing a systems biology approach to the science. When completed, GeneLab will provide the integrated software and hardware infrastructure, analytical tools and reference datasets for an assortment of model organisms. GeneLab will also provide an environment for scientists to collaborate thereby increasing the possibility for data to be reused for future experimentation. To maximize the value of data from life science experiments performed in space and to make the most advantageous use of the remaining ISS research window, GeneLab will apply an open access approach to conducting spaceflight experiments by generating, and sharing the datasets derived from these biological studies in space.Onboard the ISS, a wide variety of model organisms will be studied and returned to Earth for analysis. Laboratories on the ground will analyze these samples and provide genomic, transcriptomic, metabolomic and proteomic data. Upon receipt, NASA will conduct data quality control tasks and format raw data returned from the omics centers into standardized, annotated information sets that can be readily searched and linked to spaceflight metadata. Once prepared, the biological datasets, as well as any analysis completed, will be made public through the GeneLab Space Bioinformatics System webb as edportal. These efforts will support a collaborative research environment for spaceflight studies that will closely resemble environments created by the Department of Energy (DOE), National Center for Biotechnology Information (NCBI), and other institutions in additional areas of study, such as cancer and environmental biology. The results will allow for comparative analyses that will help scientists around the world take a major leap forward in understanding the effect of microgravity, radiation, and other aspects of the space environment on model organisms. These efforts will speed the process of scientific sharing, iteration, and discovery.

Space Life Science↗

Building a FAIR data ecosystem for incorporating single-cell transcriptomics data into agricultural genome to phenome research

Introduction The agriculture genomics community has numerous data submission standards available, but the standards for describing and storing single-cell (SC, e.g., scRNA- seq) data are comparatively underdeveloped. Methods To bridge this gap, we leveraged recent advancements in human genomics infrastructure, such as the integration of the Human Cell Atlas Data Portal with Terra, a secure, scalable, open-source platform for biomedical researchers to access data, run analysis tools, and collaborate. In parallel, the Single Cell Expression Atlas at EMBL-EBI offers a comprehensive data ingestion portal for high-throughput sequencing datasets, including plants, protists, and animals (including humans). Developing data tools connecting these resources would offer significant advantages to the agricultural genomics community. The FAANG data portal at EMBL-EBI emphasizes delivering rich metadata and highly accurate and reliable annotation of farmed animals but is not computationally linked to either of these resources. Results Herein, we describe a pilot-scale project that determines whether the current FAANG metadata standards for livestock can be used to ingest scRNA-seq datasets into Terra in a manner consistent with HCA Data Portal standards. Importantly, rich scRNA-seq metadata can now be brokered through the FAANG data portal using a semi-automated process, thereby avoiding the need for substantial expert curation. We have further extended the functionality of this tool so that validated and ingested SC files within the HCA Data Portal are transferred to Terra for further analysis. In addition, we verified data ingestion into Terra, hosted on Azure, and demonstrated the use of a workflow to analyze the first ingested porcine scRNA-seq dataset. Additionally, we have also developed prototype tools to visualize the output of scRNA-seq analyses on genome browsers to compare gene expression patterns across tissues and cell populations. This JBrowse tool now features distinct tracks, showcasing PBMC scRNA-seq alongside two bulk RNA-seq experiments. Discussion We intend to further build upon these existing tools to construct a scientist-friendly data resource and analytical ecosystem based on Findable, Accessible, Interoperable, and Reusable (FAIR) SC principles to facilitate SC-level genomic analysis through data ingestion, storage, retrieval, re-use, visualization, and comparative annotation across agricultural species.

Genetics & Heredity↗

Afterglow Polarization from Off-axis Gamma-Ray Burst Jets

As we further our studies on gamma-ray bursts (GRBs), via both theoretical models and observational tools, more and more options begin to open for exploration of its physical properties. As GRBs are transient events primarily dominated by synchrotron radiation, it is expected that the synchrotron photons emitted by GRBs should present some degree of polarization throughout the evolution of the burst. Whereas observing this polarization can still be challenging due to the constraints on observational tools, especially for short GRBs, it is paramount that the groundwork is laid for the day we have abundant data. In this work, we present a polarization model linked with an off-axis spreading top-hat jet synchrotron scenario in a stratified environment with a density profile n(r) ∝ r ⁻k . We present this model's expected temporal polarization evolution for a realistic set of afterglow parameters constrained within the values observed in the GRB literature for four degrees of stratification k = 0, 1, 1.5, and 2 and two magnetic field configurations with high extreme anisotropy. We apply this model and predict polarization from a set of GRBs exhibiting off-axis afterglow emission. In particular, for GRB 170817A, we use the available polarimetric upper limits to rule out the possibility of an extremely anisotropic configuration for the magnetic field.

79 ASTRONOMY AND ASTROPHYSICS↗