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At least 199 records · Page 11

Ecological Study of Lagoons Surrounding the John F. Kennedy Space Center, Brevard County, Florida : Theses and Project Reports - Volume 2

Many of the detailed studies done in connection with Ecological Study of the Lagoons surrounding the John F. Kennedy Space Port were performed as Master's Thesis investigations by various graduate students enrolled at F. I. T. during and subsequent to the report. The scope and purpose of what we came to call "the KSC Baseline Study" caught the imagination and interest of our student body. Many who were not financially or otherwise connected with the project found their inspiration in studies that were directly connected with project, and thus added materially to the totality of the knowledge gained. An example of one such study is the first article included in this Volume, A Master's Thesis study performed by Shen Phillip Chen, who chose the site for his investigation so that his results would correlate with and extend the results of others. In addition to the Master's Theses contained in this Volume, six other graduate studies must be acknowledged here as contributing to this Report, although they have not reached the stage of final publication. Ms. Sandra Fettes has completed a study of the amounts of five trace metals in mangrove leaves from plants at various locations around the Kennedy Space Center. Mr. Bernard Cohenour has isolated and identified a number of oil consuming bacteria endemic in the waters of the Indian Rivers. Mr. Charles Waterhouse has analyzed historic data of tidal gauges in the lagoonal area and correlated it with wind field records. Mr. Renkert Meyer has measured the vertical and horizontal currents of the lagoons and is attempting an interpretation of them in terms of the wind field as a driving force. Mr. Richard Campbell has measured the rate of nitrogen fixation in both the water columns and the sediments under them in the lagoons. Mr. Craig Weiderhold has measured thl3 annual variations in the populations of benthic invertebrates in the lagoons. An integral part of the F. I. T. curriculum is a requirement that each undergraduate student perform an independent study during his Senior year and submit his results in the form and format of a professional scientific report. While none of these senior Project Reports have been included in this Volume because of space limitations, many of them aided significantly in the overall study. Typical of these were: light and dark bottle studies of the blue-green algae respiration; writing and executing computer studies comparing the water chemistry data from various sites; detection and identification of nitrogen fixation bacteria in the Indian River; measurement of dissolved oils and greases in the river, photographically recording the benthic life-forms found in the river bottom; measuring the vertical structure of drift and slope currents in too lagoons; and measurement of water level variations along the long axis of a lagoon as a function of wind stress. These articles are published as a part of this Final Report in an effort to make available for the record as much of the basic data as possible. The few articles published on the Indian River have in general not contained much detailed data, so that comparisons of current status to conditions of the past are difficult or impossible. A major effort of both the Oceanographic and Ecological communities for the past decade has been the retrieval and storage of basic data in order to permit determinations of trends or changes in baseline conditions. It is believed that the information compiled here will be of great value in any future investigations.

Source record↗

Microbial Ecology of NASA Curation Clean Rooms

Clean room standards like ISO 14644 used for facilities that construct spacecraft and store returned samples do not explicitly account for microbial contamination. While there are associated ISO standards for monitoring and controlling bio-contamination in clean rooms it is not always standard practice to do so. The NASA Astromaterials Acquisition and Curation Office maintains seven separate clean labs for storing extraterrestrial samples from the Moon, meteorites, cosmic dust, asteroids, comets, solar wind particles, and microparticle impact samples. These labs are routinely monitored for particulate and trace metal contamination. However, the sample collections are either non-sterile at the time of collection (e.g., meteorites) or are no longer being used to address scientific questions that could be affected by non-sterile conditions (e.g., Lunar samples). Outside of isolated studies there has not been a systematic, longitudinal characterization of the microbial ecology of NASA curation clean rooms. In accordance with the advanced curation initiative, and to prepare for future sample return missions, we have initiated a routine microbiological monitoring program in the Antarctic Meteorite Lab. This monitoring program will be used to determine what microbes are capable of surviving in these oligotrophic environments and whether or not they are capable of altering the sample collections in any significant manner. Repeat sampling will allow us to understand how routine use of these labs affects the microbial ecology over time.

Regberg, A. B.↗

Integrating the Social, Hydrological and Ecological Dimensions of Freshwater Health: The Freshwater Health Index

Degradation of freshwater ecosystems and the services they provide is a primary cause of increasing water insecurity, raising the need for integrated solutions to freshwater management. While methods for characterizing the multi-faceted challenges of managing freshwater ecosystems abound, they tend to emphasize either social or ecological dimensions and fall short of being truly integrative. This paper suggests that management for sustainability of freshwater systems needs to consider the linkages between human water uses, freshwater ecosystems and governance. We present a conceptualization of freshwater resources as part of an integrated social-ecological system and propose a set of corresponding indicators to monitor freshwater ecosystem health and to highlight priorities for management. We demonstrate an application of this new framework -the Freshwater Health Index (FHI) - in the Dongjiang River Basin in southern China, where stakeholders are addressing multiple and conflicting freshwater demands. By combining empirical and modeled datasets with surveys to gauge stakeholders' preferences and elicit expert information about governance mechanisms, the FHI helps stakeholders understand the status of freshwater ecosystems in their basin, how ecosystems are being manipulated to enhance or decrease water-related services, and how well the existing water re-source management regime is equipped to govern these dynamics over time. This framework helps to operationalize a truly integrated approach to water resource management by recognizing the interplay between governance, stakeholders, freshwater ecosystems and the services they provide

ecosystem services↗

Southern Bhutan Ecological Forecasting II: Utilizing NASA Earth Observations to Model Land Cover Change and Elephant Wildlife Corridors in Southern Bhutan

The diverse landscapes of Bhutan host a rich biodiversity of animal and plant species. Asian elephants (Elephas maximus) are a flagship keystone wildlife species whose conservation is essential for the functioning of Bhutan’s forest ecosystems. Despite this, increasing habitat loss and human-elephant conflict continue to be detrimental to the survival of Asian elephants. The lack of information on Bhutan’s elephants and land use and land cover (LULC) trends present major challenges for Bhutan in modeling locations with suitable habitat and biological corridors for elephants. The DEVELOP Southern Bhutan Ecological Forecasting II team at NASA Goddard Space Flight Center partnered with the Bhutan Foundation, Bhutan Tiger Center, and Bhutan Ecological Society to help address this problem. The team mapped LULC in Bhutan for 2010 and 2015 by utilizing NASA Earth observations, including Landsat 5 Thematic Mapper (TM) and Landsat 8 Operational Land Imager (OLI) to acquire information on historical LULC patterns and view apparent land cover change. The team used the Linkage Pathways Tool of the Linkage Mapper Toolbox in ArcMap to derive biological corridor maps from habitat suitability model outputs of the previous term and known locations of protected areas in Bhutan. The corridor maps were used to view and assess corridor suitability and connectivity between protected parks. Project results are being provided to partners to help make informed decisions on the placement and conservation of elephant movement corridors.

Thinley Yidzin Wangden↗

Insights of Extreme Desert Ecology to the Habitats and Habitability of Mars

Desert ecosystems are a key repository for important Mars analog habitats and the extant or extinct life within them. We provide an overview of four main desert habitat types—soils, sediments, salts, and rocks—and the extreme microbiology living within them, with a particular focus on the hyperarid Atacama Desert and Dry Valleys of Antarctica, the driest and coldest limits for life on Earth. We construct habitat maps of Mars from an ecological perspective and the first estimates of study sample sizes of key habitats from historical and recent Mars orbiter and lander imagery and data. We review the lessons that can be drawn for the search for life on Mars from decades of microbial ecology work in end-member terrestrial deserts.

Deserts↗

Vegetation and vantage point influence visibility across diverse ecosystems: Implications for animal ecology

Visual information can influence animal behavior and habitat use in diverse ways. Visibility is the property that relates 3D habitat structure to accessibility of visual information. Despite the importance of visibility in animal ecology, this property remains largely unstudied. Our objective was to assess how habitat structure from diverse environments and animal position within that structure can influence visibility. We gathered terrestrial lidar data (1 cm at 10 m) in four ecosystems (forest, shrub-steppe, prairie, and desert) to characterize viewsheds (i.e., estimates of visibility based on spatially explicit sightlines) from multiple vantage points. Both ecosystem-specific structure and animal position influenced potential viewsheds. Generally, as height of the vantage point above the ground increased, viewshed extent also increased, but the relationships were not linear. In low-structure ecosystems (prairie, shrub-steppe, and desert), variability in viewsheds decreased as vantage points increased to heights above the vegetation canopy. In the forest, however, variation in viewsheds was highest at intermediate heights, and markedly lower at the lowest and highest vantage points. These patterns are likely linked to the amount, heterogeneity, and distribution of vegetation structure occluding sightlines. Our work is the first to apply a new method that can be used to estimate viewshed properties relevant to animals (i.e., viewshed extent and variability). We demonstrate that these properties differ across terrestrial landscapes in complex ways that likely influence many facets of animal ecology and behavior.

Rachel Stein↗

Elucidating the morphology and ecology of Eoandromeda octobrachiata from the Ediacaran of South Australia

Eoandromeda octobrachiata is a poorly understood Ediacaran organism, with spiral octoradial arms, found in South Australia and South China. The informal Nilpena member of the Rawnsley Quartzite, Flinders Ranges in South Australia preserves more than 200 specimens of Eoandromeda. Here we use the novel application of rotational geometric morphometrics together with palaeoenvironmental information to provide a better insight into their palaeobiology and ecology, and to address conflicting hypotheses regarding mode of life and taxonomic affinity. We find that Eoandromeda probably had a radially symmetrical shape in life, was cone shaped and had a high relief off the microbial mat. Analysis of the symmetric and asymmetric shape components showed that they deform strongly in the direction of palaeocurrent, therefore they are thought to be made of a flexible material. Almost all specimens are compressed flat. Specimens that appear to have not fully collapsed support the idea that Eoandromeda was probably cone shaped and suggest that they possibly collapsed spirally. Our shape analysis, along with observed morphological features, support a benthic rather than pelagic mode of life. Morphological and ecological inconsistencies do not fully support the hypothesis of a Ctenophora taxonomic affinity.

Tory L. Botha↗

Utilizing digitized occurrence records of Midwestern feral Cannabis sativa to develop ecological niche models

Hemp (Cannabis sativa L.) has historically played a vital role in agriculture across the globe. Feral and wild populations have served as genetic resources for breeding, conservation, and adaptation to changing environmental conditions. However, feral populations of Cannabis, specifically in the Midwestern United States, remain poorly understood. This study aims to characterize the abiotic tolerances of these populations, estimate suitable areas, identify regions at risk of abiotic suitability change, and highlight the utility of ecological niche models (ENMs) in germplasm conservation. The Maxent algorithm was used to construct a series of ENMs. Validation metrics and MOP (Mobility-oriented Parity) analysis were used to assess extrapolation risk and model performance. We also projected the final projected under current and future climate scenarios (2021–2040 and 2061–2080) to assess how abiotic suitability changes with time. Climate change scenarios indicated an expansion of suitable habitat, with priority areas for germplasm collection in Indiana, Illinois, Kansas, Missouri, and Nebraska. This study demonstrates the application of ENMs for characterizing feral Cannabis populations and highlights their value in germplasm conservation and breeding efforts. Populations of feral C. sativa in the Midwest are of high interest, and future research should focus on utilizing tools to aid the collection of materials for the characterization of genetic diversity and adaptation to a changing climate.

59 BASIC BIOLOGICAL SCIENCES↗

Sustainable Supply Chains by Integrating Life Cycle Modeling and Techno-Ecological Synergy with Application to Mitigation of Harmful Algal Blooms

Harmful algal blooms (HABs) are a challenge in water bodies across the planet and are often caused by fertilizer runoff from intensively farmed lands. This chapter describes a framework by which producers of biomass-based products could manage their supply chain to encourage mitigation of HABs and their life cycle environmental impacts, while also considering economic implications. The emphasis is on corn-based products such as ethanol in the region around Lake Erie in Northwest Ohio. It shows how wetland ecosystems could be designed synergistically with farming practices, transportation of the corn, and conversion and use of ethanol. The resulting techno-ecologically synergistic supply chains can be environmentally and economically superior to conventional techno-centric supply chains. The resulting designs can help ethanol manufacturers choose between farms, encourage farmers to adopt wetland ecosystems for intercepting and mitigating nutrient runoff, and determine the location of biorefineries for optimizing environmental and economic objectives.

air quality regulation↗

Spatial ecology of translocated raccoons

Raccoons (Procyon lotor) are routinely translocated both legally and illegally to mitigate conflicts with humans, which has contributed to the spread of rabies virus across eastern North America. The movement behavior of translocated raccoons has important ramifications for disease transmission yet remains understudied and poorly quantified. To examine the spatial ecology of raccoons following experimental translocation, we performed reciprocal 16 km-distance translocations of 30 raccoons between habitats of high and low raccoon density (bottomland hardwood and upland pine, respectively) across the Savannah River Site (SRS) in Aiken, South Carolina, USA (2018–2019). Translocation influenced patterns of raccoon space use, with translocated animals exhibiting a 13-fold increase in 95% utilization distributions (UDs) post- compared to pre-translocation (mean 95% UD 35.8 ± 36.1 km 2 vs 1.96 ± 1.17 km 2 ). Raccoons originating from upland pine habitats consistently had greater space use and larger nightly movement distances post-translocation compared to raccoons moved from bottomland hardwood habitats, whereas these differences were generally not observed prior to translocation. Estimated home ranges of male raccoons were twice the area as estimated for female raccoons, on average, and this pattern was not affected by translocation. After a transient period lasting on average 36.5 days (SD = 30.0, range = 3.25–92.8), raccoons often resumed pre-experiment movement behavior, with 95% UD sizes not different from those prior to translocation (mean = 2.27 ± 1.63km 2 ). Most animals established new home ranges after translocation, whereas three raccoons moved > 16 km from their release point back to the original capture location. Four animals crossed a 100-m wide river within the SRS post-translocation, but this behavior was not documented among collared raccoons prior to translocation. Large increases in space use combined with the crossing of geographic barriers such as rivers may lead to elevated contact rates with conspecifics, which can heighten disease transmission risks following translocation. These results provide additional insights regarding the potential impacts of raccoon translocation towards population level risks of rabies outbreaks and underscore the need to discourage mesocarnivore translocations to prevent further spread of wildlife rabies.

59 BASIC BIOLOGICAL SCIENCES↗

Soil enzymes as indicators of soil function: A step toward greater realism in microbial ecological modeling

Soil carbon (C) and nitrogen (N) cycles and their complex responses to environmental changes have received increasing attention. However, large uncertainties in model predictions remain, partially due to the lack of explicit representation and parameterization of microbial processes. One great challenge is to effectively integrate rich microbial functional traits into ecosystem modeling for better predictions. Here, using soil enzymes as indicators of soil function, we developed a competitive dynamic enzyme allocation scheme and detailed enzyme-mediated soil inorganic N processes in the Microbial-ENzyme Decomposition (MEND) model. We conducted a rigorous calibration and validation of MEND with diverse soil C-N fluxes, microbial C:N ratios, and functional gene abundances from a 12-year CO 2 × N grassland experiment (BioCON) in Minnesota, USA. In addition to accurately simulating soil CO 2 fluxes and multiple N variables, the model correctly predicted microbial C:N ratios and their negative response to enriched N supply. Model validation further showed that, compared to the changes in simulated enzyme concentrations and decomposition rates, the changes in simulated activities of eight C-N-associated enzymes were better explained by the measured gene abundances in responses to elevated atmospheric CO 2 concentration. In conclusion, our results demonstrated that using enzymes as indicators of soil function and validating model predictions with functional gene abundances in ecosystem modeling can provide a basis for testing hypotheses about microbially mediated biogeochemical processes in response to environmental changes. Further development and applications of the modeling framework presented here will enable microbial ecologists to address ecosystem-level questions beyond empirical observations, toward more predictive understanding, an ultimate goal of microbial ecology.

59 BASIC BIOLOGICAL SCIENCES↗

Foliar element determination from field survey in association with the National Ecological Observatory Network Airborne Observation Platform survey, East River, Colorado 2018

The purpose of this dataset is to support research aimed at understanding the coupling between hydrologic and biogeochemical processes at watershed scale, particularly the relationship between aboveground vegetation characteristics and subsurface soil properties. These data are intended to inform and calibrate models of catchment-scale biogeochemical fluxes, including rock-derived nutrient cycling, and they were procured to address the following questions: (1) What is the distribution of vegetation characteristics across the study catchments? (2) Are foliar concentrations of rock-derived nutrients related to underlying lithology and soil availability, or are these signals masked by biotic nutrient cycling and retention processes?This data package contains foliar elemental data collected during the 2018 National Ecological Observatory Networks (NEON) Airborne Observation Platform (AOP) imaging spectroscopy and lidar surveys in Gunnison County, Colorado. Folair samples were collected across the East River, Washington Gulch, Slate River, and Coal Creek watersheds and contain a mixture of vegetation including meadow, shrub, and tree foliar samples. The samples were processed using aqua regia digestion and analyzed for elemental determination on inductively coupled plasma optical emission spectrometry (ICP-OES).The data package includes: (1) raw foliar elemental data files in CSV and PDF formats, (2) quality control certificates in PDF format, and (3) an aggregated CSV file containing all elemental measurements compiled across samples. No specialized software is required to access or use these files.

2018 National Ecological Observatory Network Campa↗

Ecological Trait-Based Digital Categorization of Microbial Genomes for Denitrification Potential

Microorganisms encode proteins that function in the transformations of useful and harmful nitrogenous compounds in the global nitrogen cycle. The major transformations in the nitrogen cycle are nitrogen fixation, nitrification, denitrification, anaerobic ammonium oxidation, and ammonification. The focus of this report is the complex biogeochemical process of denitrification, which, in the complete form, consists of a series of four enzyme-catalyzed reduction reactions that transforms nitrate to nitrogen gas. Denitrification is a microbial strain-level ecological trait (characteristic), and denitrification potential (functional performance) can be inferred from trait rules that rely on the presence or absence of genes for denitrifying enzymes in microbial genomes. Despite the global significance of denitrification and associated large-scale genomic and scholarly data sources, there is lack of datasets and interactive computational tools for investigating microbial genomes according to denitrification trait rules. Therefore, our goal is to categorize archaeal and bacterial genomes by denitrification potential based on denitrification traits defined by rules of enzyme involvement in the denitrification reduction steps. We report the integration of datasets on genome, taxonomic lineage, ecosystem, and denitrifying enzymes to provide data investigations context for the denitrification potential of microbial strains. We constructed an ecosystem and taxonomic annotated denitrification potential dataset of 62,624 microbial genomes (866 archaea and 61,758 bacteria) that encode at least one of the twelve denitrifying enzymes in the four-step canonical denitrification pathway. Our four-digit binary-coding scheme categorized the microbial genomes to one of sixteen denitrification traits including complete denitrification traits assigned to 3280 genomes from 260 bacteria genera. The bacterial strains with complete denitrification potential pattern included Arcobacteraceae strains isolated or detected in diverse ecosystems including aquatic, human, plant, and Mollusca (shellfish). The dataset on microbial denitrification potential and associated interactive data investigations tools can serve as research resources for understanding the biochemical, molecular, and physiological aspects of microbial denitrification, among others. The microbial denitrification data resources produced in our research can also be useful for identifying microbial strains for synthetic denitrifying communities.

59 BASIC BIOLOGICAL SCIENCES↗

Relationship between isotope ratios in precipitation and stream water across watersheds of the National Ecological Observation Network

The timescales associated with precipitation moving through watersheds reveal processes that are critical to understanding many hydrologic systems. Measurements of environmental stable water isotope ratios (δ 2 H and δ 18 O) have been used as tracers to study hydrologic timescales by examining how long it takes for incoming precipitation tracers become stream discharge, yet limited measurements both spatially and temporally have bounded macroscale evaluations so far. Here in this observation driven study across North American biomes within the National Ecological Observation Network (NEON), we examined δ 18 O and δ 2 H stable water isotope in precipitation (δP) and stream water (δQ) at 26 co-located sites. With an average 54 precipitation samples and 139 stream water samples per site collected over 2014–2022, assessment of local meteoric water lines and local stream water lines showed geographic variation across North America. Taking the ratio of estimated seasonal amplitudes of δP and δQ to calculate young water fractions (F yw ), showed a Fyw range from 1% to 93% with most sites having F yw below 20%. Calculate d mean transit times (MTT) based on a gamma convolution model showed a MTT range from 0.10 to 13.2 years, with half of the sites having MTT estimates lower than 2 years. Significant correlations were found between the F yw and watershed area, longest flow length, and the longest flow length/slope. Significant correlations were found between MTT and site latitude, longitude, slope, clay fraction, temperature, precipitation magnitude, and precipitation frequency. The significant correlations between water timescale metrics and the environmental characteristics we report share some similarities with those reported in prior studies, demonstrating that these quantities are primarily driven by site or area specific factors. The analysis of isotope data presented here provides important constraints on isotope variation in North American biomes and the timescales of water movement through NEON study sites.

54 ENVIRONMENTAL SCIENCES↗

‘The Cloud is Not Not IT’: Ecological Change in Research Computing in the Cloud

Along with a number of other computing technologies, cloud computing services are increasingly being promoted as a way of enabling openness, reproducibility, and the acceleration of scientific work. While there have been a variety of studies of the cloud in terms of computing performance, there has been little empirical attention to the changes going on around cloud computing at the level of work and practice. Through a qualitative, ethnographic study, we follow a cosmology research group’s transition from a shared high performance computing cluster to a cloud computing service, and examine the cloud service as a coordinative artifact being integrated into a larger ecology of existing practices and artifacts. We find that the transition involves both change and continuity in the group’s coordinative work and maintenance work, and point out some of the effects this adoption has on the group’s larger set of practices. Finally, we discuss practical implications this has for the broader adoption of cloud computing in university-based scientific work.

97 MATHEMATICS AND COMPUTING↗

HydroEcoLSTM: A Python package with graphical user interface for hydro-ecological modeling with long short-term memory neural network

Machine learning (ML) is emerging as a promising tool for modeling hydro-ecological processes due to the increasing availability of large environmental data. However, the use of ML requires sufficient programming knowledge due to a lack of a graphical user interface (GUI). In this study, we introduced a GUI package, named HydroEcoLSTM, with the long short-term memory network (LSTM) as the core model, that allows non-ML experts to utilize their domain knowledge to construct complex ML models. We demonstrated the functionalities of HydroEcoLSTM with two practical examples, including (1) predictions of streamflow in both gauged and ungauged catchments and (2) predictions of multiple outputs (i.e., streamflow and isotope transport from two catchments). The simulation results obtained in both case experiments are satisfactory. In the first example, the average Nash–Sutcliffe Efficiency (NSE) for streamflow simulation during the testing period is 0.79 while the application of the trained model in two assumed ungauged catchments also achieves the average NSE of 0.68. In the second example, the average NSE for streamflow and instream isotope simulation during the testing period is 0.71. Ultimately, applications of HydroEcoLSTM with real-world examples demonstrate its potential use for practical applications and research without requiring extensive coding skills.

54 ENVIRONMENTAL SCIENCES↗

Hedonic Price Estimates of Lake Water Quality: Valued Attribute, Instrumental Variables, and Ecological-Economic Benefits

We estimate the effect of lake water quality on residential housing prices using cross-sectional data on water clarity from 113 lakes across the United States. An instrumental-variables approach is developed to address potential endogeneity bias in the water-clarity variable. Three lake-based physical variables serve as instrumental variables: total nitrogen concentration in lake water, total phosphorus concentration in lake water, and water temperature near the lake surface. The econometric results include three methodological findings: the instruments are valid and strong; the ordinary-least-squares estimate of the coefficient on the water-clarity variable is unbiased; and water clarity is the attribute of water quality valued in the housing market. The estimated water-clarity effect shows that a one-tenth of a meter change in water clarity leads to a one-percent change in housing price, or an elasticity of 0.20 at mean clarity. Coupling this effect with estimates of an ecological production function, a lake-specific benefit index is developed that shows the effect on housing values of bringing lakes into compliance with the US Environmental Protection Agency's regional recommendations for phosphorous concentration in lake waters.

54 ENVIRONMENTAL SCIENCES↗

Towards smarter green infrastructure: Fusing bark ecology and stemflow hydrodynamics on tree stems

A wide array of bark surfaces sheath wooded plants in rural and urban areas alike. Much work has examined the function and role of bark in different contexts and different environs, including urban areas, finding that it is rich in life and can play a role in the transfer of water and matter to the ground surface. Accordingly, this paper presents a first step to weld and fuse bark ecology and stemflow hydrodynamics. It is an effort to develop a physically-based understanding of the transport of water and matter (e.g., solutes, particulates, microorganisms) along tree stems using relevant equations to allow a more informed consideration of bark in green infrastructure initiatives. In particular, the hydrodynamical equations are based on the conservation of water mass, conservation of momentum, and conservation of scalar mass. These equations, coupled with contemplation of corticular life, underpin and substantiate bark’s unifying role as a modulator and cultivator. By elucidating the ‘black box’ of the tree stem and utilizing the formulations set forth in this paper, urban foresters and planners can develop green infrastructure to help advance ecosystem services and sustainability development goals (SDG), especially SDG 11 and SDG 15.

60 APPLIED LIFE SCIENCES↗