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All sourcesNASA NTRSDOE OSTI
DOE OSTI2026

Exploring life’s hidden majority: microbial dark matter symposium highlights

The Microbial Dark Matter Symposium held on August 28–29, 2025, in Laguna Beach, Orange County, CA, convened a multidisciplinary group of scientists to address the vast unknowns in microbial life—from uncultured taxa and uncharacterized proteins to elusive viruses and spacefaring microbes. Set against a scenic coastal backdrop, the symposium highlighted advances in single-cell genomics, proximity ligation sequencing, and artificial intelligence-ready bioinformatics, while also probing the limits of microbial persistence, metabolism, and ecological distribution. Sessions explored microbial dark matter from multiple dimensions: cultivability, where new strategies are enabling recovery of elusive microbes; functional ambiguity, where metagenomic dark zones are illuminated by computational annotation; and genomic representation, where single-cell methods bridge gaps left by shotgun community sequencing. Researchers shared breakthroughs in identifying atmospheric microbiomes, “dark oxygen” production in groundwater ecosystems, and microbial survival on the International Space Station. The symposium emphasized integration of methods, disciplines, and ecosystems, advancing a collective push to illuminate the microbial dark matter on Earth and beyond. By highlighting emerging tools, pressing questions, and cross-domain insights, the symposium underscored the need for collaborative, open, and adaptive approaches to study the microbial unknown. The meeting marks a pivotal moment in microbiology, where cultivating knowledge of the uncultivated promises transformative understanding of life, everywhere.

Podar, Mircea [ORNL] (ORCID:0000000327760205)↗
DOE OSTI2024

Light Dark Matter Analysis Using NO$\nu$A Near Detector

Dark matter (DM) is believed to account for 85$\%$ of the matter content of the Universe. The leading dark matter candidate is the WIMP (weakly interacting massive particles). Light dark matter (LDM) refers to WIMP candidates with a mass of less than 1 GeV. The concept of LDM has been developed in order to explain the 511 keV $\gamma$-rays from the galactic bulge, as observed by the INTEGRAL satellite. There are a lot of candidates for light DM, and these candidates span a wide range of potential masses and couplings to the visible sector. Probing the vast parameter space of light-dark matter requires a correspondingly broad experimental program that can include neutrino fixed target experiments. NOvA is a high luminosity long-baseline fixed-target accelerator neutrino experiment at Fermilab that can provide a potentially interesting probe in searching for signatures of DM scattering with electrons in its near detectors. We aim to search for the MeV-scale dark matter particles that might be generated within the NuMI beam and produce detectable electron scattering signals in NOvA Near Detector. In this talk, we present our analysis of the single electron events using a simulated sample and show the sensitivity of the NOvA experiment.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Neutrino constraints on inelastic dark matter captured in the Sun

Abstract The flux of neutrinos from annihilation of gravitationally captured dark matter in the Sun has significant constraints from direct-detection experiments. However, these constraints are relaxed for inelastic dark matter as inelastic dark matter interactions generate less energetic nuclear recoils compared to elastic dark matter interactions. In this paper, we explore the possibility for large volume underground neutrino experiments to detect the neutrino flux from captured inelastic dark matter in the Sun. The neutrino spectrum has two components: a mono-energetic “spike” from pion and kaon decays at rest and a broad-spectrum “shoulder” from prompt primary meson decays. We focus on detecting the shoulder neutrinos from annihilation of hadrophilic inelastic dark matter with masses in the range 4–100 GeV and the mass splittings in up to 300 keV. We determine the event selection criterion for DUNE to identify GeV-scale muon neutrinos and anti-neutrinos originating from hadrophilic dark matter annihilation in the Sun, and forecast the sensitivity from contained events. We also map the current bounds from Super-Kamiokande and IceCube on elastic dark matter, as well as the projected limits from Hyper-Kamiokande, to the parameter space of inelastic dark matter. We find that there is a region of parameter space that these neutrino experiments are more sensitive to than the direct-detection experiments. For dark matter annihilation to heavy-quarks, the projected sensitivity of DUNE is weaker than current (future) Super (Hyper) Kamiokande experiments. However, for the light-quark channel, only the spike is observable and DUNE will be the most sensitive experiment.

Astronomy & Astrophysics↗
DOE OSTI2026

Scalable dark matter searches using integrated photonics

Dark matter (DM) with masses of order an electronvolt or below can have a nonzero coupling to electromagnetism while being compatible with cosmological observations. In these models, the ambient DM behaves as a new classical source in Maxwell’s equations, which can excite potentially detectable electromagnetic (EM) fields in the laboratory. We propose a new integrated-photonics–based approach to search for dark matter candidates in the 0.1–few eV mass range. This approach offers a wide range of wavelength-scale devices like resonators and wave guides that are readily fabricated in large quantities, enabling a scalable and novel search. In particular, we demonstrate that refractive index-modulated resonators, such as etched/grooved microrings, or patterned slabs, support EM modes with efficient coupling to DM. When excited by DM, these modes are read out by coupling the resonators to a wave guide that terminates on a micron-scale–sized single photon detector, such as a single pixel of a low-noise charge-coupled device or a superconducting nanowire. We then estimate the sensitivity of this experimental concept in the context of axionlike particle and dark photon models of DM, demonstrating that nanophotonic confinement and scalability can extend dark matter sensitivity into previously unexplored parameter space.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
NASA NTRS1994

A hydrodynamic approach to cosmology: The mixed dark matter cosmological scenario

We compute the evolution of spatially flat, mixed cold and hot dark matter models containing both baryonic matter and two kinds of dark matter. Hydrodynamics is treated with a highly developed Eulerian hydrodynamic code (see Cen 1992). A standard particle-mesh (PM) code is also used in parallel to calculate the motion of the dark matter components. We adopt the following parameters: h equivalent to (sub 0)/100 km/s Mpc(exp -1) = 0.5, OMEGA(sub C) = 0.3, and OMEGA(sub B) = 0.06, with amplitude of the perturbation spectrum fixed by the Cosmic Background Explorer Satellite (COBE) Dark Matter Radiation (DMR) measurements (Smoot et al. 1992) being sigma (sub 8) = 0.67. Four different boxes are simulated with box sizes of L = (64, 16, 4, 1) h(exp -1) Mpc, respectively, the two small boxes providing good resolution but little valid information due to absence of large-scale power. We use 128(exp 3) approximate 10(exp 6.3) baryonic cells, 128(exp .3) cold dark matter particles, and 2 x 128(exp 3) hot dark matter particles. In addition to the dark matter we follow separately six baryonic species (H, H(+), He, He(+), He(++), e(-)) with allowance for both (nonequilibrium) collisional and radiative ionization in every cell. The background radiation field is also followed in detail with allowance made for both continuum and line processes, to allow nonequilibrium heating and cooling processes to be followed in detail. The mean final Zeldovich-Sunyaev y parameter is estimated to be y Bar = (5.4 + or - 2.7) x 10(exp -7) below currently attainable observations, with a rms fluctuation of approximately delta bar y = (0.6 + or - 3.0) x 10(exp -7) on arcminute scales. The rate of galaxy formation peaks at an even later epoch (z approximate 0.3) than in the standard (OMEGA = 1, sigma sub 8 = 0.67) cold dark matter (CDM) model (z approximate 0.5) and, at a redshift of z = 4, is nearly a factor of 100 lower than for the CDM model with the same value of sigma sub 8. With regard to mass function, the smallest objects are stabilized against collapse by thermal energy: the mass-weighted mass spectrum has a broad peak in the vicinity of M(sub B) = 10(exp 9.5) solar mass with a reasonable fit to the Schechter luminosity function if the ratio of baryon mass to blue light is approximately 4. In addition, one very large PM simulation was made in a box with size (320 h(exp - 1) Mpc) containing 3 x 200(exp 3) = 10(exp 7.4) particles. Utilizing this simulation we find that the model yields a cluster mass function which is about a factor of 4 higher than observed, but a cluster-cluster correlation length marginally lower than observed, but that both are closer to observations than in the (COBE) normalized CDM model. The one-dimensional pairwise velocity dispersion is 605 + or - 8 km/s at 1/h separation, lower than that of the DCM model normalized to COBE, but still significant higher than observations (Davis & Peebles 1983). A plausible velocity bias b(sub v) = 0.8 + or - 0.1 on this scale will reduce but not remove the discrepancy. The velocity auto-correlat ion function has a coherence length of 40/h Mpc, which is somewhat lower than the observed counterpart. In all these respects the model would be improved by decreasing the cold fraction of the dark OMEGA(sub CDM)/ (OMEGA(sub CDM) + OMEGA(sub HDB). But formation of galaxies and clusters of galaxies is much later in this model than in COBE-normalized CDM, perhaps too late. To improve on these constraints a larger ratio of OMEGA(sub CDM)/ (OMEGA(sub CDM) + OMEGA(sub HDM)) is required than the value of 0.67 adopted here. It does not seem possible to find a value for this ratio which would satisfy all tests. Overall, the model is similar both on large and intermediate scales to the standard CDM model normalized to the same value of sigma(sub B), but the problem with regard to late formation of galaxies is more severe in this model than in that CDM model. Adding hot dark matter, significantly improves the ability of the COBE-normalized CDM scenario to fit existing observations, but the model is in fact not as good as the CDM model with the same sigma(sub 8) and is still probably unsatisfactory with regard to several critical tests.

Cen, Renyue↗
DOE OSTI2024

Thermalization and annihilation of dark matter in neutron stars

The capture of dark matter, and its subsequent annihilation, can heat old, isolated neutron stars. In order for kinetic heating to be achieved, the captured dark matter must undergo sufficient scattering to deposit its kinetic energy in the star. We find that this energy deposit typically occurs quickly, for most of the relevant parameter space. In order for appreciable annihilation heating to also be achieved, the dark matter must reach a state of capture-annihilation equilibrium in the star. We show that this can be fulfilled for all types of dark matter - baryon interactions. This includes cases where the scattering or annihilation cross sections are momentum or velocity suppressed in the non-relativistic limit. Importantly, we find that capture-annihilation equilibrium, and hence maximal annihilation heating, can be achieved without complete thermalization of the captured dark matter. For scattering cross sections that saturate the capture rate, we find that capture-annihilation equilibrium is typically reached on a timescale of less than 1 year for vector interactions and 10 4 years for scalar interactions.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2023

Pushing the limits of detectability: mixed dark matter from strong gravitational lenses

One of the frontiers for advancing what is known about dark matter lies in using strong gravitational lenses to characterize the population of the smallest dark matter haloes. There is a large volume of information in strong gravitational lens images – the question we seek to answer is to what extent we can refine this information. To this end, we forecast the detectability of a mixed warm and cold dark matter scenario using the anomalous flux ratio method from strong gravitational lensed images. The halo mass function of the mixed dark matter scenario is suppressed relative to cold dark matter but still predicts numerous low-mass dark matter haloes relative to warm dark matter. Since the strong lensing signal receives a contribution from a range of dark matter halo masses and since the signal is sensitive to the specific configuration of dark matter haloes, not just the halo mass function, degeneracies between different forms of suppression in the halo mass function, relative to cold dark matter, can arise. Here, we find that, with a set of lenses with different configurations of the main deflector and hence different sensitivities to different mass ranges of the halo mass function, the different forms of suppression of the halo mass function between the warm dark matter model and the mixed dark matter model can be distinguished with 40 lenses with Bayesian odds of 30:1.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Faint galaxies and small halos : probes of galaxy formation and dark matter

This thesis addresses the intersection of dark matter and galaxy formation physics by modeling the faintest galaxies in the Universe in a cosmological context. In the first part of this work, I present an empirical model for the connection between faint galaxies and small dark matter halos. Combining this model with tailored cosmological simulations of Milky Way analogs and state-of-the-art satellite galaxy observations yields new insights into dwarf galaxy formation and hierarchical structure formation. Next, I show that these same dwarf galaxy observations place stringent constraints on microphysical dark matter properties including its warmth, self-interactions, Standard Model interactions, minimum particle mass, and formation epoch. Finally, I describe a unified framework that combines dark matter constraints from dwarf galaxies and strong gravitational lensing, paving the way for next-generation surveys of small-scale cosmic structure to deliver unprecedented insights into dark matter physics.

Nadler, Ethan Oliver↗
DOE OSTI2021

Skipper CCDs for the search of a daily modulation of Dark Matter signal in the DMSQUARE experiment

The Dark Matter Daily Modulation experiment (DMSQUARE) seeks for dark matter interactions with a Skipper CCD. It is currently running at surface level in Bariloche, Argentina, and will be moved to a shallow underground site at Sierra Grande, Argentina in November 2021. The low threshold achieved by Skipper CCDs allows to search for electron recoil events with an ionization energy down to 1.2 eV. In order to extract a potential dark matter signal from noise at the single electron level, we propose to search for a diurnal modulation of events, resulting from the potential interaction of the dark matter wind with the particles in the Earth. Depending on the model, mass and cross-section, this modulation can be maximum at 40deg of latitude in the Southern Hemisphere, where DMSQUARE is operated. In this article we present the experiment, report preliminary results with a prototype Skipper CCD taking data at surface level and comment on future prospects.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2024

New constraints on ultraheavy dark matter from the LZ experiment

Searches for dark matter with liquid xenon time projection chamber experiments have traditionally focused on the region of the parameter space that is characteristic of weakly interacting massive particles, ranging from a few GeV / c 2 to a few TeV / c 2 . Models of dark matter with a mass much heavier than this are well motivated by early production mechanisms different from the standard thermal freeze-out, but they have generally been less explored experimentally. In this work, we present a reanalysis of the first science run of the LZ experiment, with an exposure of 0.9 tonne × yr , to search for ultraheavy particle dark matter. The signal topology consists of multiple energy deposits in the active region of the detector forming a straight line, from which the velocity of the incoming particle can be reconstructed on an event-by-event basis. Zero events with this topology were observed after applying the data selection calibrated on a simulated sample of signal-like events. New experimental constraints are derived, which rule out previously unexplored regions of the dark matter parameter space of spin-independent interactions beyond a mass of 10 17 GeV / c 2 . Published by the American Physical Society 2024

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2025

Dark showers from sneaky dark matter

We present a minimal composite dark matter model, based on a SU(Nd) dark sector with nf dark quarks and a heavy t-channel mediator. For nf ≥ 4, the dark flavor symmetry guarantees the stability of a subset of the dark pions, which serve as our dark matter candidates. Their relic abundance is determined by dark sector annihilation with the remaining dark pions, which are unstable and decay. Due to their degenerate masses, the annihilation cross section is suppressed at low temperatures, thereby avoiding stringent constraints from indirect detection and opening up the GeV mass window. The decaying dark pions are naturally long lived. We obtain limits on the model from semi-visible or emerging jet searches and estimate the reach of future probes.

ALPs↗
DOE OSTI2020

Dark photon dark matter in the presence of inhomogeneous structure

Dark photon dark matter will resonantly convert into visible photons when the dark photon mass is equal to the plasma frequency of the ambient medium. In cosmological contexts, this transition leads to an extremely efficient, albeit short-lived, heating of the surrounding gas. Existing work in this field has been predominantly focused on understanding the implications of these resonant transitions in the limit that the plasma frequency of the Universe can be treated as being perfectly homogeneous, \ie neglecting inhomogeneities in the electron number density. In this work we focus on the implications of heating from dark photon dark matter in the presence of inhomogeneous structure (which is particularly relevant for dark photons with masses in the range $10^{-15} \, {\rm eV} \, \lesssim m_{A^\prime} \lesssim 10^{-12}$ eV), emphasizing both the importance of inhomogeneous energy injection, as well as the sensitivity of cosmological observations to the inhomogeneities themselves. More specifically, we derive modified constraints on dark photon dark matter from the Ly-$\alpha$ forest, and show that the presence of inhomogeneities allows one to extend constraints to masses outside of the range that would be obtainable in the homogeneous limit, while only slightly relaxing their strength. We then project sensitivity for near-future cosmological surveys that are hoping to measure the 21cm transition in neutral hydrogen prior to reionization, and demonstrate that these experiments will be extremely useful in improving sensitivity to masses near $\sim 10^{-14}$ eV, potentially by several orders of magnitude. Finally, we discuss implications for reionization, early star formation, and late-time $y$-type spectral distortions, and show that probes which are inherently sensitive to the inhomogeneous state of the Universe could resolve signatures unique to the light dark photon dark matter scenario, and thus offer a fantastic potential for a positive detection.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2020

Confronting Dark Matter with the Multiwavelength Sky (Final Technical Report)

The existence of dark matter is a key clue to new fundamental physics, yet its nature and non-gravitational interactions remain mysterious. In the course of this five-year project (spanning July 2015 - July 2020), we have developed novel analysis techniques to separate dark matter signals from astrophysical backgrounds in the inner Galaxy, and measured hitherto-unknown properties of the Fermi Bubbles and the Galactic Center excess in gamma-rays from the inner Milky Way. We have improved predictions for the spatial distribution and energy spectra of photon signals from dark matter, and set new limits from astrophysical and cosmological datasets on dark matter inhabiting complex dark sectors, multi-particle dark matter annihilation, and tiny primordial black holes. We have successfully adapted effective field theory techniques from collider physics to make high-precision predictions for signals from heavy weakly-interacting DM, allowing us to essentially exclude the possibility that DM is a thermal wino, and have discovered novel theoretical and observational implications for dark matter bound states and self-interactions. We have developed a comprehensive public code package for modeling the effects of exotic energy injections, such as from dark matter annihilation and decay, on the ionization and temperature history of the early cosmos, and have used these tools to set robust, powerful and broadly-applicable constraints on models of light dark matter.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2019

Constraints on bosonic dark matter from ultralow-field nuclear magnetic resonance

The nature of dark matter, the invisible substance making up over 80% of the matter in the universe, is one of the most fundamental mysteries of modern physics. Ultralight bosons such as axions, axion-like particles, or dark photons could make up most of the dark matter. Couplings between such bosons and nuclear spins may enable their direct detection via nuclear magnetic resonance (NMR) spectroscopy: As nuclear spins move through the galactic dark-matter halo, they couple to dark matter and behave as if they were in an oscillating magnetic field, generating a dark-matter–driven NMR signal. As part of the cosmic axion spin precession experiment (CASPEr), an NMR-based dark-matter search, we use ultralow-field NMR to probe the axion-fermion “wind” coupling and dark-photon couplings to nuclear spins. No dark matter signal was detected above background, establishing new experimental bounds for dark matter bosons with masses ranging from 1.8 × 10 -16 to 7.8 × 10 -14 eV.

Science & Technology - Other Topics↗
DOE OSTI2026

Simplified spin dependence in dark matter direct detection

The interactions of dark matter with Standard Model particles can be systematically studied in the language of effective field theories. We investigate dark matter interactions with Standard Model particles, including spin-dependent interactions, for direct detection experiments and demonstrate that, although the scattering rate generally depends on multiple types of material response functions, certain linear combinations of these material response functions vanish if the initial and final electronic states share the same Hamiltonian. We also find that several other response functions vanish in parity-symmetric materials, making these systems as simple as isotropic detectors in some respects. Finally, we present the scattering rate for an anisotropic, possibly chiral detector, for generic dark matter-electron spin interactions. These relations reduce the number of independent response functions needed, thereby simplifying the computational complexity for a broad class of dark matter models. Our results provide a complete and efficient toolkit for analyzing electron recoil signals in diverse detector materials.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2022

Calibrating the scintillation and ionization responses of xenon recoils for high-energy dark matter searches

We report liquid xenon-based direct detection dark matter experiments have recently expanded their searches to include high-energy nuclear recoil events as motivated by effective field theory dark matter and inelastic dark matter interaction models, but few xenon recoil calibrations above 100 keV are currently available. In this work, we measured the scintillation and ionization yields of xenon recoils up to 426 keV. The experiment uses 14.1 MeV neutrons to scatter off xenon in a compact liquid xenon time projection chamber and produce quasimonoenergetic xenon recoils between 39 and 426 keV. We report the xenon recoil responses and their electric field dependence for recoil energies up to 306 keV; due to the low event statistics and the relatively mild field dependence, the yield values at higher energies are reported as the average of xenon responses for electric fields between 0.2 and 2.0 kV/cm. This result will enable xenon-based dark matter experiments to significantly increase their high-energy dark matter sensitivities by including energy regions that were previously inaccessible due to lack of calibrations.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2026

Search for GeV-scale dark matter from the Galactic Center with IceCube-DeepCore

Models describing dark matter as a novel particle often predict that its annihilation or decay into Standard Model particles could produce a detectable neutrino flux in regions of high dark matter density, such as the Galactic Center. In this work, we search for these neutrinos using ∼9 years of IceCube-DeepCore data with an event selection optimized for energies between 15 to 200 GeV. We considered several annihilation and decay channels and dark matter masses ranging from 15 GeV up to 8 TeV. No significant deviation from the background expectation from atmospheric neutrinos and muons was found. The most significant result was found for a dark matter mass of 201.6 GeV annihilating into a pair of bb¯ quarks assuming the Navarro–Frenk–White halo profile with a post-trial significance of 1.08σ. We present upper limits on the thermally averaged annihilation cross section of the order of 10-24 cm3 s-1, as well as lower limits on the dark matter decay lifetime up to 1026 s for dark matter masses between 5 GeV up to 8 TeV. These results strengthen the current IceCube limits on dark matter masses above 20 GeV and provide an order of magnitude improvement at lower masses. In addition, they represent the strongest constraints from any neutrino telescope on GeV-scale dark matter and are among the world-leading limits for several dark matter scenarios.

Abbasi, R↗
DOE OSTI2021

Prospects for measuring dark matter microphysics with observations of dwarf spheroidal galaxies

Dark matter annihilation in dwarf spheroidal (dSph) galaxies near the Milky Way has the potential to produce a detectable signature in gamma-rays. The amplitude of this signal depends on the dark matter density in a dSph, the dark matter particle mass, the number of photons produced in an annihilation, and the possibly velocity-dependent dark matter annihilation cross section. We argue that if the amplitude of the annihilation signal from multiple dSphs can be measured, it is possible to determine the velocity-dependence of the annihilation cross section. However, we show that doing so will require improved constraints on the dSph density profiles, including control of possible sources of systematic uncertainty. Making reasonable assumptions about future improvements, we make forecasts for the ability of current and future experiments — including Fermi, CTA and AMEGO — to constrain the dark matter annihilation velocity dependence.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗