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At least 199 records · Page 11

Microbial Tracking-2, a metagenomics analysis of bacteria and fungi onboard the International Space Station

The International Space Station (ISS) is a unique and complex built environment with the ISS surface microbiome originating from crew and cargo or from life support recirculation in an almost entirely closed system. The Microbial Tracking 1 (MT-1) project was the first ISS environmental surface study to report on the metagenome profiles without using whole-genome amplification. The study surveyed the microbial communities from eight surfaces over a 14-month period. The Microbial Tracking 2 (MT-2) project aimed to continue the work of MT-1, sampling an additional four flights from the same locations, over another 14 months. Eight surfaces across the ISS were sampled with sterile wipes and processed upon return to Earth. DNA extracted from the processed samples (and controls) were treated with propidium monoazide (PMA) to detect intact/viable cells or left untreated and to detect the total DNA population (free DNA/compromised cells/intact cells/viable cells). DNA extracted from PMA-treated and untreated samples were analyzed using shotgun metagenomics. Samples were cultured for bacteria and fungi to supplement the above results. Staphylococcus sp. and Malassezia sp. were the most represented bacterial and fungal species, respectively, on the ISS. Overall, the ISS surface microbiome was dominated by organisms associated with the human skin. Multi-dimensional scaling and differential abundance analysis showed significant temporal changes in the microbial population but no spatial differences. The ISS antimicrobial resistance gene profiles were however more stable over time, with no differences over the 5-year span of the MT-1 and MT-2 studies. Twenty-nine antimicrobial resistance genes were detected across all samples, with macrolide/lincosamide/streptogramin resistance being the most widespread. Metagenomic assembled genomes were reconstructed from the dataset, resulting in 82 MAGs. Functional assessment of the collective MAGs showed a propensity for amino acid utilization over carbohydrate metabolism. Co-occurrence analyses showed strong associations between bacterial and fungal genera. Culture analysis showed the microbial load to be on average 3.0 × 10 5 cfu/m 2 . Utilizing various metagenomics analyses and culture methods, we provided a comprehensive analysis of the ISS surface microbiome, showing microbial burden, bacterial and fungal species prevalence, changes in the microbiome, and resistome over time and space, as well as the functional capabilities and microbial interactions of this unique built microbiome. Data from this study may help to inform policies for future space missions to ensure an ISS surface microbiome that promotes astronaut health and spacecraft integrity.

59 BASIC BIOLOGICAL SCIENCES↗

Single-shot picosecond pump coherent Rayleigh scattering thermometry

A single-shot coherent Rayleigh scattering (CRS) technique capable of measurement times less than 10 ns is presented. Here, the use of a mode-locked picosecond pump laser yields repeatable electrostrictive forcing compared to previous CRS experiments using unseeded nanosecond pump pulses, which are beset by shot-to-shot variations. Quantitative measurements are achieved by dispersing the CRS signal onto an EMCCD sensor using a virtually imaged phased array and comparing the experimental spectra to an existing kinetic model with a least-squares fitting routine. Measurements are demonstrated at ambient and low-pressure (2 Torr), low-temperature (100 K) conditions where the CRS measurement is within the collisionless regime. Single-shot statistics indicated precision and accuracy within 4% at ambient conditions and within 8% at low density and temperature conditions. This single-shot CRS technique is a powerful diagnostic tool with potential for multi-parameter measurements in complex flow environments, including high-speed aerodynamic ground test facilities.

Senior, William Charles Bowman [Sandia National La↗

Crewmember microbiome may influence microbial composition of ISS habitable surfaces

The International Space Station (ISS) is a complex built environment physically isolated from Earth. Assessing the interplay between the microbial community of the ISS and its crew is important for preventing biomedical and structural complications for long term human spaceflight missions. In this study, we describe one crewmember’s microbial profile from body swabs of mouth, nose, ear, skin and saliva that were collected at eight different time points pre-, during and post-flight. Additionally, environmental surface samples from eight different habitable locations in the ISS were collected from two flights. Environmental samples from one flight were collected by the crewmember and samples from the next flight were collected after the crewmember departed. The microbial composition in both environment and crewmember samples was measured using shotgun metagenomic sequencing and processed using the Livermore Metagenomics Analysis Toolkit. Ordination of sample to sample distances showed that of the eight crew body sites analyzed, skin, nostril, and ear samples are more similar in microbial composition to the ISS surfaces than mouth and saliva samples; and that the microbial composition of the crewmember’s skin samples are more closely related to the ISS surface samples collected by the crewmember on the same flight than ISS surface samples collected by other crewmembers on different flights. In these collections, species alpha diversity in saliva samples appears to decrease during flight and rebound after returning to Earth. This is the first study to compare the ISS microbiome to a crewmember’s microbiome via shotgun metagenomic sequencing. We observed that the microbiome of the surfaces inside the ISS resemble those of the crew’s skin. These data support future crew and ISS microbial surveillance efforts and the design of preventive measures to maintain crew habitat onboard spacecraft destined for long term space travel.

59 BASIC BIOLOGICAL SCIENCES↗

Enabling AI in synthetic biology through Construction File specification

The Construction File (CF) specification establishes a standardized interface for molecular biology operations, laying a foundation for automation and enhanced efficiency in experiment design. It is implemented across three distinct software projects: PyDNA_CF_Simulator, a Python project featuring a ChatGPT plugin for interactive parsing and simulating experiments; ConstructionFileSimulator, a field-tested Java project that showcases 'Experiment' objects expressed as flat files; and C6-Tools, a JavaScript project integrated with Google Sheets via Apps Script, providing a user-friendly interface for authoring and simulation of CF. The CF specification not only standardizes and modularizes molecular biology operations but also promotes collaboration, automation, and reuse, significantly reducing potential errors. The potential integration of CF with artificial intelligence, particularly GPT-4, suggests innovative automation strategies for synthetic biology. While challenges such as token limits, data storage, and biosecurity remain, proposed solutions promise a way forward in harnessing AI for experiment design. This shift from human-driven design to AI-assisted workflows, steered by high-level objectives, charts a potential future path in synthetic biology, envisioning an environment where complexities are managed more effectively.

59 BASIC BIOLOGICAL SCIENCES↗

Mauka Energy FEVER Tool Dataset

Mauka Energy’s dataset, developed under the Forestry Electric Vehicle Energy Routing (FEVER) project and funded by the U.S. Department of Energy’s Small Business Innovation Research program, is a high-resolution geospatial resource designed to support energy modeling for electric log trucks in complex forestry environments. The dataset integrates detailed spatial and road network data to enable accurate simulation of vehicle performance across varied terrain. At its core, the dataset incorporates lidar-derived elevation models, road alignments, and surface classifications from Oregon State University’s McDonald-Dunn Research Forest. These data capture fine-scale variations in slope, curvature, and surface conditions across forest road systems, allowing for vehicle-level analysis of energy consumption and recovery. The dataset also includes data collected on the surrounding public and private road networks in Benton County, Oregon, used in real-world haul routes. These connecting segments provide critical context for modeling transitions between forest operations and regional transportation infrastructure, incorporating attributes such as grade profiles, elevation change, and speed constraints. This combined dataset underpins the development of Mauka Energy’s rolldown tool, which quantifies energy use and regenerative braking potential on downhill and variable-grade segments. By leveraging high-resolution terrain and road data, the FEVER project enables more accurate assessment of electric vehicle feasibility and performance in forestry applications.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

American WAKE experimeNt (AWAKEN)

The national laboratories, directed by the U.S. Department of Energy Wind Energy Technologies Office, will organize, design, and execute a landmark international wake observation and validation campaign known as the American WAKE experimeNt or AWAKEN. This document describes the vision and purpose for AWAKEN and describes some of the organizational activities that have taken place to date. The driving need for this experimental campaign is that wake interactions are among the least understood physical phenomena in wind plants today, leading to unexpected power and financial losses. New observation data gathered will be used to further validate wind plant models and lead to both improved layout and more optimal operation of wind farms with greater power production and improved reliability, ultimately leading to lower wind energy costs. This campaign will occur in the U.S. Midwest, where the largest concentration of wind farms in the U.S. is located and where significant future growth is most likely. The field campaign will provide a data set that is unique among wake studies, based on location, scope, and observational fidelity: most wake studies have largely focused on offshore wind farms, which are not currently critical to the U.S. wind energy supply, and with a limited number of observations. While AWAKEN is focused on land-based wind farms in flat terrain, many of the observations will help researchers understand fundamental wake behavior applicable in both offshore and complex terrain environments.

17 WIND ENERGY↗

DUNE and MINERvA Flux Studies and a Measurement of the Charged-Current Quasielastic Antineutrino Scattering Cross Section with $<E_{\nu}> \sim 6 $ GeV on a CH Target

One of the biggest open questions in physics is the prevalence of matter over anti-matter in the present universe. One of possible answers lies in the violation of charge and parity symmetry in the lepton sector that would favor matter over anti-matter so that the universe becomes dominated by matter over time. Recent results from neutrino experiments like T2K and NOvA have indicated that there is CP violation in the leptonic sector \citep{Abe:2019vii}. However, the precise measurement of CP violation will require an unprecedented level of accuracy in these oscillation experiments. One of the challenges lies with the systematic uncertainties that come with the measurements, which include uncertainties related to the neutrino flux modeling, interaction modeling and detector related systematic uncertainties.\\ The first part of this thesis will go through the challenges related to flux uncertainties. It will discuss the flux uncertainties related to hadron production in the LBN F beamli ne for the DUNE experiment. Hadron production models used in flux simulations vary widely depending on the simulation and choice of physics models used to simulate the hadron production. This thesis will explain the method of using existing hadron production data to constrain these uncertainties, a method which has been used in MINERvA experiment.\\ The second part of the thesis goes through the effect of possible flux mismodeling and MINERvA's approach to address this effect. In doing so, we discovered that the suspected mismodeling could also result from incorrect estimation of the energy scale of the muons. This project not only demonstrated the challenges of flux modeling but also led to a novel approach of using neutrino energy spectra to understand the correlation between detector and neutrino beamline parameters. \\ A high statistics measurement of the anti-neutrino scattering cross section in the charged current quasielastic (CCQE) channel is the final part of the th esis. Th e 2-body interactions in this channel have the advantage of allowing reconstruction of the interaction kinematics from the outgoing muon trajectory which can be measured very well. Double differential cross sections as a function of muon kinematics, one of the deliverables of this analysis, will help future oscillation experiments understand their data. The cross-section as a function of the four-momentum transferred from the leptonic system to the hadronic system ($Q^{2}_{QE}$) can be used to test models used in simulating antineutrino interactions and the nuclear effects that can modify the predicted cross sections. Nuclear effects arise from the complex nuclear environment and both modify the initial scattering and change the fate of the particles produced from in neutrino-nucleus interactions. Study of nuclear effects will help to understand the structure of the atomic nucleus and its impact on neutrino interactions.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Evaluation of Structural Lattices for a Davis Gun Earth Penetrator Impact Experiment

The advanced materials team investigated the use of additively manufactured metallic lattice structures for mitigating impact response in a Davis gun earth penetrator impact experiment. High-fidelity finite element models were developed and validated with quasistatic experiments. These models were then used to simulate the response of such lattices when subjected to the acceleration loads expected in the Davis gun experiment. Results reveal how the impact mitigation performance of lattices can change drastically at a certain relative density. Based on these observations, an experiment deck was designed to probe the response of lattices with different relative densities during the Davis gun phase 2 shots. The expected performance of these lattices is predicted before testing based on simulation results. The results of the Davis gun phase 2 shots are expected to provide data which will be used to assess the predictive capability of the finite element simulations in such a complex impact environment.

36 MATERIALS SCIENCE↗

Permutation-adapted complete and independent basis for atomic cluster expansion descriptors

In many recent applications, particularly in the field of atom-centered descriptors for interatomic potentials, tensor products of spherical harmonics have been used to characterize complex atomic environments. When coupled with a radial basis, the atomic cluster expansion (ACE) basis is obtained. However, symmetrization with respect to both rotation and permutation results in an overcomplete set of ACE descriptors with linear dependencies occurring within blocks of functions corresponding to particular generalized Wigner symbols. All practical applications of ACE employ semi-numerical constructions to generate a complete, fully independent basis. While computationally tractable, the resultant basis cannot be expressed analytically, is susceptible to numerical instability, and thus has limited reproducibility. Here we present a procedure for generating explicit analytic expressions for a complete and independent set of ACE descriptors. The procedure uses a coupling scheme that is maximally symmetric w.r.t. permutation of the atoms, exposing the permutational symmetries of the generalized Wigner symbols, and yields a permutation-adapted rotationally and permutationally invariant basis (PA-RPI ACE). Theoretical support for the approach is presented, as well as numerical evidence of completeness and independence. A summary of explicit enumeration of PA-RPI functions up to rank 6 and polynomial degree 32 is provided. The PA-RPI blocks corresponding to particular generalized Wigner symbols may be either larger or smaller than the corresponding blocks in the simpler rotationally invariant basis. Finally, we demonstrate that basis functions of high polynomial degree persist under strong regularization, indicating the importance of not restricting the maximum degree of basis functions in ACE models a priori.

74 ATOMIC AND MOLECULAR PHYSICS↗

In-situ thermodynamics measurements at metal oxides-solution interfaces using Flow Adsorption Microcalorimetry.

Mineral-fluid interfaces are the principal sites of geochemical processes near Earth’s surface, hosting chemical reactions that play a fundamental role in (bio)geochemical cycles and in the fate and transport of anthropogenic contaminants, and that effectively control the compositions of soil and water environments. These complex interfaces are critical for our energy and environmental future. The mineral-fluid interface has been studied in an unprecedented level of detail with both experimental and computational approaches, separately and in combination. However, conspicuously missing from studies of the mineral-fluid interface are direct measurements of the energies of ion sorption and exchange. The literature on energetics and enthalpies of exchange, adsorption, dissolution, precipitation, and surface protonation reactions, especially those directly supported by experimental data, remains scarce despite their fundamental nature. The overarching goal of this project is to complete a systematic study of the thermodynamics properties of interfacial reactions at four MO surfaces (Rutile (α-TiO2), Quartz (SiO2), boehmite (γ-AlOOH) and goethite (α-FeOOH)) through the application and construction of novel flow adsorption microcalorimetry techniques and instrumentations. These unique and specialized microcalorimeters will operate at various temperatures and solution chemical compositions allowing for in-situ measurements across metal oxides and ligands of various characteristics. The overall research goal will be accomplished by completing the following three specific objectives (O): O1) Determine the energetics of surface protonation and deprotonation, ion exchange and ligand sorption reactions; O2) Investigate the surface charge thermodynamic properties under a range of temperature and solution chemical compositions; and O3) Develop predictive trends of the interplay between MO structure, surface coverage and surface reactivity. In addition to key thermodynamics parameters, calorimetric measurements provide a wealth of mechanistic information about reactions energetics and kinetics, surface charge characteristics, and structure-reactivity or selectivity relationships, all obtained in-situ and in real-time. This report includes science highlights from various projects completed over the performance period of the project. Also listed are dissemination opportunities, people supported on the grant and the impact on available physical resources and the discipline as a whole.

58 GEOSCIENCES↗

Use of Stable Mercury Isotopes to Assess Mercury and Methylmercury Transformation and Transport across Critical Interfaces from the Molecular to the Watershed Scale (Final Report)

This project titled “Use of Stable Mercury Isotopes to Assess Mercury and Methylmercury Transformation and Transport across Critical Interfaces from the Molecular to the Watershed Scale” represents a collaborative effort between the University of Michigan (Jason Demers, PI) and Oak Ridge National Laboratory (Scott Brooks, co-I). Much has been learned about mercury (Hg) cycling in stream ecosystems, and East Fork Poplar Creek (EFPC) in particular, through decades of previous research. Nevertheless, some of the most fundamental questions regarding the sources of bioavailable Hg and its transformation to toxic methylmercury (MeHg) have remained unanswered. These fundamental questions include: (1) what are the sources and biogeochemical processes that lead to the input of dissolved Hg to stream water across critical subsurface interfaces within stream ecosystems, and EFPC in particular? and (2) what are the sources and biogeochemical processes that control the production and fate of bioaccumulative MeHg within stream ecosystems, and in EFPC in particular? To address these fundamental questions, our project aimed to couple laboratory experiments and field observations, both utilizing natural abundance Hg stable isotope techniques, to identify the processes responsible for generating mobile, bioavailable dissolved Hg from recalcitrant legacy sources within critical subsurface zones (e.g., streambed hyporheic zone, riparian floodplain subsurface). We used the isotopic signature of this bioavailable dissolved Hg to track its mobilization across these critical interfaces in order to link diffuse subsurface sources of dissolved Hg with increases in surface water dissolved Hg flux measured at the watershed scale. Additionally, our research aimed to determine the isotopic composition of MeHg within these same critical subsurface zones. We directly assessed the isotopic composition of MeHg within biota in order to gain insight into which subsurface sources of inorganic Hg and toxic MeHg are available for bioaccumulation within the EFPC ecosystem. Net fluxes of dissolved Hg along the flow path of EFPC were shown to vary spatially and temporally. In the Upper EFPC, within the Y12 boundary, stream water flux of dissolved Hg consistently decreased between the outfall and the downstream boundary of Y12 (57% ± 29%, 1SD). Within the Upper EFPC, an assessment of Hg isotopic composition suggested that losses were strongly reaction-driven, although isotopic diagnostics did not conform to any known processes. Downstream of Y12, in the upper reach of the Lower EFPC, dissolved Hg fluxes tended to increase during the dormant season (net gain of 11-120%), and decrease during the growing season (net loss of 23% +/- 18%, 1SD). In the downstream-most reach of Lower EFPC, dissolved Hg fluxes increased by 12-108% in 9 out of 10 monthly assessments. Overall, diffuse fluxes from the non-Y12 watershed accounted for 34% (+/- 17%, 1SD) of all dissolved Hg exported during base flow. Within Lower EFPC, an assessment of Hg isotopic composition was consistent with the contribution of diffuse Hg inputs from high-concentration hotspots within riparian floodplains and streambed hyporheic pore water. To investigate remobilization of recalcitrant Hg from legacy sediment sources, we developed procedures that coupled isotopic analysis with sequential extractions of streambed sediment. We found that the proportion of weakly-bound Hg within EFPC streambed sediment was relatively small, but could still account for a large proportion of the annual flux of dissolved Hg from EFPC. These sequential extractions also showed that this weakly-bound Hg fraction could be replenished from the much larger fraction of recalcitrant Hg in sediment. The isotopic composition of these weakly-bound and remobilized recalcitrant Hg fractions within the sediment was consistent with high-concentration dissolved Hg hotspots within hyporheic pore water. Thus, this research provided novel evidence that legacy mercury sources within streambed sediment could provide an ongoing contribution of dissolved Hg to surface waters. Finally, we developed new methods for the direct determination of the MeHg isotopic composition of organisms, which allowed a more direct evaluation of inorganic Hg and MeHg sources accumulating in the food web. We found that fish and aquatic invertebrates in both EFPC and a regional background site obtained inorganic Hg and MeHg from multiple isotopically distinct sources, including sediment, suspended particulates, and periphyton. Photodemethylation was found to be an important reaction influencing MeHg dynamics at both sites. However, the balance of microbial methylation and demethylation processes differed between the two streams, with fractionation resulting from methylation and demethylation processes being relatively in balance within the regional background site, whereas microbial methylation appeared to be dominant over microbial demethylation within the EFPC ecosystem. Broadly, the application of Hg isotopic analysis in this study led to numerous novel insights regarding the biogeochemical cycling of Hg in stream ecosystems. This research project promoted the development of two new approaches, including the coupling of sequential extractions with Hg isotopic analysis to assess remobilization of recalcitrant Hg within sediments, and a new method for the isotopic analysis of MeHg isolated from environmental samples. Both of these efforts represent advances in capacity for the field of mercury isotopic analysis and environmental assessment. Overall, this study demonstrates that the application of Hg stable isotope techniques continues to provide new insights into the biogeochemical cycling of Hg in complex aquatic environments, both within the EFPC and beyond.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Frequency Emitter Geolocation Using Signal Strength Fingerprinting Informed By 3D Propagation Modeling

This work focuses on the problem of RF geolocation in complex multipath environments. Using 3D electromagnetic propagation modeling to characterize environments of interest will enable more accurate RF geolocation. Specifically, a path-loss radio map can be generated in simulation for use in received signal strength indicators (RSSI) fingerprinting, or pattern matching. RSSI fingerprinting is an example of data-based method that takes site specific information into account which should allow for better performance than other model-based methods that use a generalized model of electromagnetic propagation. This modeling capability will also be used to evaluate the relative performance of RSSI fingerprinting, pathloss model based RSSI methods such as differential received signal strength circles (DRSS), RSSI joint gaussian estimation, and time-difference of arrival (TDOA). New methods using this simulation derived electromagnetic characterization could improve the efficacy of currently deployed and future RF spectral monitoring solutions. Wireless InSite developed by Remcom is used as the simulation tool of choice in this work. An indoor location is simulated with a grid of fixed receivers and a grid of transmit locations. Using the output of the Wireless InSite simulation the response from a given transmit location to a given receive location can be generated. During the first year of the project various geolocation methods evaluated on purely synthetic, but realistic, data. The second year focused on testing and validating the efficacy of simulation informed RF geolocation using two physical testbeds. This work has shown that data-based approaches are more accurate than model-based ones at the expensive of requiring measured or simulated site-specific training data.

47 OTHER INSTRUMENTATION↗

Deciphering controls on metal migration within floodplains: The critical role of redox environments on metal-organic complexes (Final Technical Report)

Our project elucidates the effect of differing hydrologic regimes on nutrient and contaminant dynamics within floodplains, and we explore the impact of varying sources of hydrologic extremes on nutrient-contaminant fate and transport. We use a combination of field measurements and laboratory experiments to examine the relationships between redox conditions, functionality of dissolved organic matter, metal speciation (specifically examining metal-ligand complexes), and nutrient-contaminant fate, particularly during hydrologic extremes. Floodplain biogeochemical variation through climatic extremes and beaver-induced hydrologic variation provide a unique look at controls on nutrient-contaminant fate and transport. Through our work, we have developed a novel approach that provides an unprecedented ability to resolve aqueous metal-organic complexes that reveals metal binding preferences for natural organic matter based on metal-ligand chemistry. We have developed novel methods to separate, quantify, and characterize organic-metal complexes in natural DOM that pair with field experiments comprising floodplains having systematic variation in biogeochemical conditions. We also developed a comprehensive computational approach for molecular characterization of metal-organic species that merges LC-coupled high resolution (HR) mass spectrometry and ICP-MS analysis. Importantly, we show that beaver dams have an outsized influence on hydrologic extremes, dwarfing the impact of climatic variation, that ultimate control biogeochemical conditions and water quality. Additionally, beaver dams impose a hydrologic driver that leads to improved, river water quality by divert water through soils and the hyporheic zone. We further show that iron(III) minerals have a dominant control on carbon mineralization across floodplains independent of hydrologic conditions, and that iron and aluminum associations with organic matter are a controlling factor in carbon mineralization. Ultimately, our work is helping to advancing a robust predictive understanding of how hydrologic changes in watersheds affect water quality and carbon and metal cycling in riparian zones.

54 ENVIRONMENTAL SCIENCES↗

Control of Energy Transport and Transduction in Photosynthetic Down-Conversion (Final Technical Report)

The research conducted under this contract focused on three areas related to control of energy transport and transduction in natural and synthetic light harvesting systems: (1) experimental and theoretical studies exploring the use of ultrafast laser pulse shaping to manipulate the initially excited states of multi-chromophore biological light harvesting networks and influence the early time energy transfer and electronic and vibrational relaxation pathways, (2) fundamental theoretical developments aimed at computing and analyzing the dynamics of excitations of complex heterogeneous environments and nano structures, and (3) joint experimental and theoretical design studies of artificial light harvesting materials exploring the influence of incorporating different types of organic layers in low dimensional lead halide perovskite materials on their hot carrier relaxation dynamics, and theoretical studies of the design of nano structured light harvesting antenna systems. These research projects supported the training of three theoretical and computational graduate students, one experimental graduate student, and one experimental post-doctoral researcher. The research has been published in seven peer reviewed papers and presented at several international meetings and workshops.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Real-time neutron multiplicity and source localization for criticality safety during fuel debris removal

Advancing neutron detection and analysis techniques for complex radiation environments is an ongoing focus in nuclear instrumentation and monitoring. This proposal presents research and development of a generalized real-time neutron monitoring and analysis system, applicable to any detector capable of producing time-tagged neutron count data. While the work is demonstrated using the Neutron Multiplication Analysis Detector (NoMAD), a modular 15-tube helium-3 (He-3) array, due to its availability, spatial resolution, and flexible deployment, the methods developed are extensible to other systems, including organic scintillators and fast digital detectors. This research investigates two complementary analytical techniques for real-time characterization of neutron emitting sources: neutron multiplicity estimation based on the Hage-Cifarelli formalism and spatial localization using supervised machine learning applied to spatial count rate patterns. These methods are designed to operate under dynamic, evolving conditions such as fuel debris retrieval or reactor startup, where neutron-emitting material geometries may be partially unknown or changing over time. By integrating statistical neutron emission data with spatial localization, this research aims to develop and evaluate methods for real time neutron monitoring, source characterization, and material verification. Key contributions include implementation of a low-latency data pipeline for continuous neutron multiplicity analysis, development and validation of machine learning models for spatial inference, and experimental evaluation of system performance under variable measurement conditions. The outcomes are intended to support applications in nuclear safeguards, verification, emergency response, and reactor startup.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Track Reconstruction using Graph Neural Networks in the EMPHATIC Experiment

Track reconstruction is essential for extracting physics observables from detector data in high-energy and nuclear physics experiments. In this work, we investigate the use of graph neural networks (GNNs) to reconstruct particle momentum in the EMPHATIC experiment using simulated data. The model takes raw hit information from the silicon strip detectors (SSDs) as input and is trained to predict momentum components and the scattering angle of the particle. We describe the GNN architecture, training procedure, and performance metrics, and present results showing improved resolution in momentum reconstruction. These results demonstrate the potential of GNN-based approaches in track reconstruction tasks within complex detector environments like EMPHATIC.

Bhattarai, Aayush [Notre Dame U.]↗

Risks to the DOW Mission From Global Advanced Energy Adoption

Advanced energy technologies have the potential to enhance energy security and resilience; however, they can introduce new vulnerabilities even as they mitigate existing ones. This dichotomy highlights that both action and inaction carry strategic risks in a contested and logistically complex operating environment. Regardless of U.S. civilian or military adoption of such technologies, key allies and adversaries are on adoption paths that will impact the U.S. military at the tactical, operational, and strategic level. The global adoption of advanced energy technologies presents the potential for both positive and negative consequences for U.S. Department of Defense (DOD) missions in the next 10-20 years. Three categories of risk drivers are presented in this analysis: infrastructure-related, adoption-related, and response-related. Each risk type can generate consequences for DOD, including power disruptions, suboptimal DOD mission performance and operational effectiveness, higher costs and shortages for both legacy and advanced energy technologies, and loss of U.S. influence and strategic deterrent value. Specific impacts could include tactical impacts, operational impacts, and strategic impacts. Mitigation strategies could include energy resource and technology planning, cybersecurity, supply chain resilience, workforce development, exercises and simulations, investments in commercialized technology, operational testing and pilot programs, research into emerging technologies, partnerships and working groups, common standards, budget planning, and repurposing infrastructure.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Cully Community Solar

This report summarizes the outcomes, accomplishments, challenges, and lessons learned from Verde’s Cully Community Solar project, funded by the U.S. Department of Energy’s Solar Energy Technologies Office. The project focused on advancing community solar development through engineering, permitting, interconnection, ecological site design, workforce development, and community engagement. During the project period, Verde and its partners successfully developed and commissioned a 1.25 MW ground-mounted community solar system while also advancing planning for additional community solar capacity and developing approaches that can inform future projects in complex urban environments.

14 SOLAR ENERGY↗