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At least 199 records · Page 11

Plant Chlorophyll Content Imager with Reference Detection Signals

A portable plant chlorophyll imaging system is described which collects light reflected from a target plant and separates the collected light into two different wavelength bands. These wavelength bands, or channels, are described as having center wavelengths of 700 nm and 840 nm. The light collected in these two channels is processed using synchronized video cameras. A controller provided in the system compares the level of light of video images reflected from a target plant with a reference level of light from a source illuminating the plant. The percent of reflection in the two separate wavelength bands from a target plant are compared to provide a ratio video image which indicates a relative level of plant chlorophyll content and physiological stress. Multiple display modes are described for viewing the video images.

Spiering, Bruce A.↗

Plant Responses to Increased UV-B Radiation: A Research Project

Ozone decrease implies more ultraviolet-B (UV-B) radiation reaching the surface of the Earth. Increased UV-B radiation triggers responses by living organisms. Despite the large potential impacts on vegetation, little is known about UV-B effects on terrestrial ecosystems. Long-term ecological studies are needed to quantify the effects of increased UV radiation on terrestrial ecosystems, asses the risks, and produce reliable data for prediction. Screening pigments are part of one of the protective mechanism in plants. Higher concentrations of screening pigments in leaves may be interpreted as a response to increased UV radiation. If the screening effect is not sufficient, important molecules will be disturbed by incoming radiation. Thus, genetics, photosynthesis, growth, plant and leaf shape and size, and pollen grains may be affected. This will have an impact on ecosystem dynamics, structure and productivity. It is necessary to monitor selected terrestrial ecosystems to permit detection and interpretation of changes attributable to global climate change and depleted ozone shield. The objectives of this project are: (1) To identify and measure indicators of the effects of increased solar UV-B radiation on terrestrial plants; (2) to select indicators with the greatest responses to UV-B exposure; (3) to test, adapt or create ecosystem models that use the information gathered by this project for prediction and to enhance our understanding of the effects of increased UV-B radiation on terrestrial ecosystems. As a first step to achieve these objectives we propose a three-year study of forest and steppe vegetation on the North slope of the Brooks Range (within the Arctic circle, in Alaska), in the Saguaro National Monument (near Tucson, Arizona) and in the forests and steppes of Patagonia (Argentina). We selected (1) vegetation north of the Polar Circle because at 70N there is 8% risk of plant damage due to increased UV-B radiation; (2) the foothills of Catalina Mountains because there is anecdotal evidence of plant damage on the saguaros that has been linked to increased UV radiation, and (3) the forests of Nothofagus spp. and the steppe of Patagonia where the risk of plant damage at 35S is 5% and increases to as much as 15% at 55S due to increased UV-B radiation. Measurements of UV-B radiation impinging on the surface at 55S largely exceed the predicted UV-B radiation values at 50 latitude and 0% ozone depletion. Preliminary HPLC analyses of UV-B absorbing compounds in Nothofagus antartica, N. pumilio, N. betuloides and Rumex sp. in natural conditions show species-specific patterns. The spectrum of N. antartica grown at 38S differs significantly from that of N. antartica in natural conditions in Ushuaia (55S). These results suggest that the selected main area (Patagonia) is appropriate for assessing the problem and its magnitude and that Nothofagus is appropriate for our study.

DAntoni, H. L.↗

Making Sense of Plant Health

Ciencia, Inc. created a new device, known as a Portable Photosynthesis Analyzer, or Phase Fluorometer, that provides real-time data about the photochemical efficiency of phytoplankton and other plant forms. The commercial version of this technology is used for photosynthesis research and offers major benefits to the field of life science. This new instrument is the first portable instrument of its kind. Through a license agreement with Ciencia, Oriel Instruments, of Stratford, Connecticut, manufactures and markets the commercial version of the instrument under the name LifeSense.TMLifeSense is a 70 MHz single-frequency fluorometer that offers unrivaled capabilities for fluorescence lifetime sensing and analysis. LifeSense provides information about all varieties of photosynthetic systems. Photosynthesis research contributes important health assessments about the plant, be it phytoplankton or a higher form of plant life. With its unique sensing capabilities, LifeSense furnishes data regarding the yield of a plant's photochemistry, as well as its levels of photosynthetic activity. The user can then gain an extremely accurate estimate of the plant's chlorophyll biomass, primary production rates, and a general overview of the plant's physiological condition.

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Detecting Plant Stress

Through an exclusive patent license from NASA Stennis Space Center, Spectrum Technologies, Inc., has developed a hand-held tool that helps farmers, foresters and other growers detect unhealthy crops before the human eye can see the damage. Developed by two NASA researchers, the Observer,TM shows the viewer which plants are under stress through multispectral imaging, a process that uses specific wavelengths of the light spectrum to obtain information about objects-in this case, plants. With this device, several wavelengths of light collect information about the plant and results are immediately processed and displayed. NASA research found that previsible signs of stress, such as such as a lack of nutrients, insufficient water, disease, or insect damage, can be detected by measuring the chlorophyll content based on light energy reflected from the plant. The Observer detects stress up to 16 days before deterioration is visible to the eye. Early detection provides an opportunity to reverse stress and save the plant. The hand-held, easily operated unit works in both natural and artificial light, making it suitable for outdoor or indoor planting.

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Space Magnets Attracting Interest on Earth: Applications of Physical and Biological Techniques In the Study of Gravisensing and Response System of Plants

The BioTube/Magnetic Field Apparatus (MFA) research is designed to provide insight into the organization and operation of the gravity sensing systems of plants and other small organisms. This experiment on STS-107 uses magnetic fields to manipulate sensory cells in plant roots, thus using magnetic fields as a tool to study gravity-related phenomena. The experiment will be located in the SPACEHAB module and is about the size of a household microwave oven. The goal of the experiment is to improve our understanding of the basic phenomenon of how plants respond to gravity. The BioTube/MFA experiment specifically examines how gravitational forces serve as a directional signal for growth in the low-gravity environment of space. As with all basic research, this study will contribute to an improved understanding of how plants grow and will have important implications for improving plant growth and productivity on Earth. In BioTube/MFA, magnetic fields will be used to determine whether the distribution of subcellular starch grains, called amyloplasts, within plant cells predicts the direction in which roots will grow and curve in microgravity.

Karl H Hasenstein↗

The Plant Research Unit: An International Space Station Habitat

The Plant Research Unit (PRU) is one of six life science habitats being developed as part of the Space Station Biological Research Program. The PRU is designed for experiments in microgravity and will utilize the ISS Centrifuge Facility to provide gravity levels between microgravity and 29. The PRU will provide and control all aspects of a plant s needs in a nearly closed system. In other words, the shoot and root environments will not be open to the astronaut s environment except for experiment maintenance such as planting, harvesting and plant sampling. This also means that all lighting, temperature and humidity control, "watering," and air filtering and cleaning .must be done within strict limitations of volume, weight, power, and crew time while at the same time providing a very high level of reliability and a service life in excess of 10 years. The PRU will contain two plant chambers 31.5 cm tall, each with independent control of temperature, humidity, light level and photoperiod, CO2 level, nutrient and water delivery, and video and data acquisition. The PRU is currently in the preliminary design phase and a number of subsystem components have been prototyped for testing, including the temperature and humidity control systems, the plant chambers, the LED lighting system, the atmospheric control system and a variety of nutrient delivery systems. The LED prototype provides independent feedback control of 5 separate spectral bands and variable output between 0 and 1000 micro-mol sq m/sec. The water and nutrient delivery system (WNDS) prototypes have been used to test particulate based, thin film, and gel-based WNDS configurations.

Morrow, Robert↗

Development of a Self-calibrating Dissolved Oxygen Microsensor Array for the Monitoring and Control of Plant Growth in a Space Environment

Plant experiments in space will require active nutrient delivery concepts in which water and nutrients are replenished on a continuous basis for long-term growth. The goal of this study is to develop a novel microsensor array to provide information on the dissolved oxygen environment in the plant root zone for the optimum control of plant cultivation systems in the space environment. Control of water and oxygen is limited by the current state-of-the-art in sensor technology. Two capabilities of the new microsensor array were tested. First, a novel in situ self-diagnosis/self-calibration capability for the microsensor was explored by dynamically controlling the oxygen microenvironment in close proximity to an amperometric dissolved oxygen microsensors. A pair of integrated electrochemical actuator electrodes provided the microenvironments based on water electrolysis. Miniaturized thin film dissolved oxygen microsensors on a flexible polyimide (Kapton(Registered Trademark)? substrate were fabricated and their performances were tested. Secondly, measurements of dissolved oxygen in two representative plant growth systems were made, which had not been performed previously due to lack of proper sensing technology. The responses of the oxygen microsensor array on a flexible polymer substrate properly reflected the oxygen contents on the surface of a porous tube nutrient delivery system and within a particulate substrate system. Additionally, we demonstrated the feasibility of using a 4-point thin film microprobe for water contents measurements for both plant growth systems. mechanical flexibility, and self-diagnosis. The proposed technology is anticipated to provide a reliable sensor feedback plant growth nutrient delivery systems in both terrestrial environment and the microgravity environment during long term space missions. The unique features of the sensor include small size and volume, multiple-point sensing,

Kim, Chang-Soo↗

Flexible Microsensor Array for the Monitoring and Control of Plant Growth System

Testing for plant experiments in space has begun to explore active nutrient delivery concepts in which water and nutrients are replenished on a continuous basis for long-term growth. The goal of this study is to develop a novel microsensor array to provide information on the dissolved oxygen environment in the plant root zone for the optimum control of hydroponics and solid substrate plant cultivation systems in the space environment. Miniaturized polarographic dissolved oxygen sensors have been designed and fabricated on a flexible Kapton (trademark) (polyimide) substrate. Two capabilities of the new microsensor array were explored. First, measurements of dissolved oxygen in the plant root zone in hydroponics and solid substrate culture systems were made. The microsensor array was fabricated on a flexible substrate, and then cut out into a mesh type to make a suspended array that could be placed either in a hydroponics system or in a solid substrate cultivation system to measure the oxygen environments. Second, the in situ self-diagnostic and self-calibration capability (two-point for oxygen) was adopted by dynamically controlling the microenvironment in close proximity to the microsensors. With a built-in generating electrode that surrounds the microsensor, two kinds of microenvironments (oxygen-saturated and oxygen-depleted phases) could be established by water electrolysis depending on the polarity of the generating electrode. The unique features of the new microsensor array (small size, multiple sensors, flexibility and self-diagnosis) can have exceptional benefits for the study and optimization of plant cultivation systems in both terrestrial and microgravity environments. The in situ self-diagnostic and self-calibration features of the microsensor array will also enable continuous verification of the operability during entire plant growth cycles. This concept of automated control of a novel chemical monitoring system will minimize crew time required for maintenance, as well as reduce volume, mass, and power consumption by eliminating bulky diagnosis systems including calibrant (fluid and gas) reservoir and flow system hardware.

Kim, Chang-Soo↗

Plant Responses to Rarified Atmospheres

Reduced atmospheric pressures will likely be used to minimize mass and engineering requirements for plant growth habitats used in extraterrestrial applications. This report provides a brief survey of key literature related to responses of plants to atmospheric variables and a broad rationale for designing minimal atmospheres for future plant growth structures on the Martian surface. The literature and recent work suggest that atmospheric pressure limits for normal plant function are likely to be 10 kPa or perhaps slightly lower. At Kennedy Space Center, a chamber with high vacuum capability was used to design and begin construction of a system for testing plant responses to reduced pressure atmospheres. A test rack with lighting provided by 3, high-pressure sodium vapor lamps was built to conduct measurements of short-term plant responses. Initial experiments with lettuce showed that a pressure of 10 kPa resulted in a 6.1-fold increase in the rate of water loss compared to water loss at ambient pressure (101 kPa).

Corey, Kenneth A.↗

Plants Clean Air and Water for Indoor Environments

Wolverton Environmental Services Inc., founded by longtime government environmental scientist B.C. "Bill" Wolverton, is an environmental consulting firm that gives customers access to the results of his decades of cutting-edge bioremediation research. Findings about how to use plants to improve indoor air quality have been published in dozens of NASA technical papers and in the book, "How to Grow Fresh Air: 50 Houseplants That Purify Your Home or Office." The book has now been translated into 12 languages and has been on the shelves of bookstores for nearly 10 years. A companion book, "Growing Clean Water: Nature's Solution to Water Pollution," explains how plants can clean waste water. Other discoveries include that the more air that is allowed to circulate through the roots of the plants, the more effective they are at cleaning polluted air; and that plants play a psychological role in welfare in that people recover from illness faster in the presence of plants. Wolverton Environmental is also working in partnership with Syracuse University, to engineer systems consisting of modular wicking filters tied into duct work and water supplies, essentially tying plant-based filters into heating, ventilation, and air conditioning (HVAC) systems. Also, the company has recently begun to assess the ability of the EcoPlanter to remove formaldehyde from interior environments. Wolverton Environmental is also in talks with designers of the new Stennis Visitor's Center, who are interested in using its designs for indoor air-quality filters

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Diffuse-Illumination Systems for Growing Plants

Agriculture in both terrestrial and space-controlled environments relies heavily on artificial illumination for efficient photosynthesis. Plant-growth illumination systems require high photon flux in the spectral range corresponding with plant photosynthetic active radiation (PAR) (400 700 nm), high spatial uniformity to promote uniform growth, and high energy efficiency to minimize electricity usage. The proposed plant-growth system takes advantage of the highly diffuse reflective surfaces on the interior of a sphere, hemisphere, or other nearly enclosed structure that is coated with highly reflective materials. This type of surface and structure uniformly mixes discrete light sources to produce highly uniform illumination. Multiple reflections from within the domelike structures are exploited to obtain diffuse illumination, which promotes the efficient reuse of photons that have not yet been absorbed by plants. The highly reflective surfaces encourage only the plant tissue (placed inside the sphere or enclosure) to absorb the light. Discrete light sources, such as light emitting diodes (LEDs), are typically used because of their high efficiency, wavelength selection, and electronically dimmable properties. The light sources are arranged to minimize shadowing and to improve uniformity. Different wavelengths of LEDs (typically blue, green, and red) are used for photosynthesis. Wavelengths outside the PAR range can be added for plant diagnostics or for growth regulation

May, George↗

NASA Space Biology Plant Research for 2010-2020

The U.S. National Research Council (NRC) recently published "Recapturing a Future for Space Exploration: Life and Physical Sciences Research for a New Era" (http://www.nap.edu/catalog.php?record id=13048), and NASA completed a Space Biology Science Plan to develop a strategy for implementing its recommendations ( http://www.nasa.gov/exploration/library/esmd documents.html). The most important recommendations of the NRC report on plant biology in space were that NASA should: (1) investigate the roles of microbial-plant systems in long-term bioregenerative life support systems, and (2) establish a robust spaceflight program of research analyzing plant growth and physiological responses to the multiple stimuli encountered in spaceflight environments. These efforts should take advantage of recently emerged analytical technologies (genomics, transcriptomics, proteomics, metabolomics) and apply modern cellular and molecular approaches in the development of a vigorous flight-based and ground-based research program. This talk will describe NASA's strategy and plans for implementing these NRC Plant Space Biology recommendations. New research capabilities for Plant Biology, optimized by providing state-of-the-art automated technology and analytical techniques to maximize scientific return, will be described. Flight experiments will use the most appropriate platform to achieve science results (e.g., ISS, free flyers, sub-orbital flights) and NASA will work closely with its international partners and other U.S. agencies to achieve its objectives. One of NASA's highest priorities in Space Biology is the development research capabilities for use on the International Space Station and other flight platforms for studying multiple generations of large plants. NASA will issue recurring NASA Research Announcements (NRAs) that include a rapid turn-around model to more fully engage the biology community in designing experiments to respond to the NRC recommendations. In doing so, NASA's Space Biology research will optimize ISS research utilization, develop and demonstrate technology and hardware that will enable new science, and contribute to the base of fundamental knowledge that will facilitate development of new tools for human space exploration and Earth applications. By taking these steps, NASA will energize the Space Biology user community and advance our knowledge of the effect of the space flight environment on living systems.

Levine, H. G.↗

Preparation for the Proof of Concept Flight of the Veggie Plant Growth Chamber

Veggie is a small plant growth chamber designed and built by ORBITEC that will fly to the International Space Station on SpaceX-3, scheduled for the summer of 2013. Ultimately Veggie will be used for research, education and outreach, and crew recreation. We want to demonstrate the functionality of this hardware by testing a scenario that could allow the crew to grow and consume fresh vegetables. Veggie will be collapsed and transported flat in a cargo transfer bag, and deployed on orbit, where it will be installed in an EXPRESS rack. The chamber consists of three subsystems: an LED light cap, a transparent bellows, and a root mat reservoir assembly. The bellows and flexible support arms allow the distance between plants and light cap to be adjusted for different ages and types of plants. Researchers at Kennedy Space Center and ORBITEC have been working to develop the plant growth interfaces for the proof of concept flight. We have developed a rooting pillow, consisting of a small bag containing media, time release fertilizer, seeds, and a wicking surface to conduct water from the root mat reservoir. Prototype pillows have been tested and results have influenced the design of flight pillows, which will be modified for microgravity from flight-approved materials. Several studies have been conducted selecting species and comparing media types in analog systems. Water content seems to be the most important factor differentiating media types in these small growth volumes (100 mL). Media type also influenced microbial levels on plants. Since produce sanitizing agents are not currently approved for growing food crops on orbit, plants and media types having very low microbial levels are being selected. Lettuce, mizuna, and other salad greens typically have microbial counts less than 10(exp 4) colony forming units and thus are good candidates for spaceflight. As we approach flight verification testing, we will finalize species, media selection, harvesting, and microbial sampling procedures. Next steps include testing of Veggie flight and ground hardware and associated equipment. This research was funded by NASA.

Massa, Gioia↗

Key Gaps for Enabling Plant Growth in Future Missions

Growing plants to provide food or psychological benefits to crewmembers is a common vision for the future of human spaceflight, often represented in media and in serious concept studies. The complexity of controlled environment agriculture, and plant growth in microgravity have and continue to be the subject of dedicated scientific research. However, actually implementing these systems in a way that will be cost effective, efficient, and sustainable for future space missions is a complex, multi-disciplinary problem. Key questions exist in many areas: human medical research in nutrition and psychology, horticulture, plant physiology and microbiology, multi-phase microgravity fluid physics, hardware design and technology development, and system design, operations and mission planning. This paper describes key knowledge gaps identified by a multi-disciplinary working group within the National Aeronautics and Space Administration (NASA). It also begins to identify solutions to the simpler questions identified by the group based on work initiated in 2017. Growing plants to provide food or psychological benefits to crewmembers is a common vision for the future of human spaceflight, often represented in media and in serious concept studies. The complexity of controlled environment agriculture, and plant growth in microgravity have and continue to be the subject of dedicated scientific research. However, actually implementing these systems in a way that will be cost effective, efficient, and sustainable for future space missions is a complex, multi-disciplinary problem. Key questions exist in many areas: human medical research in nutrition and psychology, horticulture, plant physiology and microbiology, multi-phase microgravity fluid physics, hardware design and technology development, and system design, operations and mission planning. This paper describes key knowledge gaps identified by a multi-disciplinary working group within the National Aeronautics and Space Administration (NASA). It also begins to identify solutions to the simpler questions identified by the group based on work initiated in 2017.

Anderson, Molly↗

Growing Beyond Earth; Students Exploring Plant Varieties for Future Space Exploration

Future space exploration and long duration space flight will pose an array of challenges to the health and wellbeing of astronauts. Since 2015, Fairchild Tropical Botanic Garden (FTBG), in partnership with NASA's Veggie team, has been testing edible crops for space flight potential through a series of citizen science experiments. FTBG's interest in classroom-based science projects, along with NASA's successful operation of the Veggie system aboard the International Space Station (ISS), led to a NASA-FTBG partnership that gave rise to the Growing Beyond Earth STEM Initiative (GBE). Established in 2015, GBE now involves 131 middle and high school classrooms in South Florida, all conducting simultaneous plant science experiments. The results of those experiments (both numeric and visual) are directly shared with the space food production researchers at KSC. Through this session, we will explore the successful classroom implementation and integration into the curriculum, how the data is being used and the impact of the project on participating researchers, teachers, and students. Participating schools were supplied with specialized LED-lit growth chambers, mimicking the Veggie system on ISS, for growing edible plants under similar physical and environmental constraints. Research protocols were provided by KSC scientists, while edible plant varieties were selected mainly by the botanists at FTBG. In a jointly-led professional development workshop, participating teachers were trained to conduct GBE experiments in their classrooms. Teachers were instructed to not only teach basic botany concepts, but to also demonstrate practical applications of math, physics and chemistry. As experiments were underway, students shared data on plant germination, growth, and health in an online spreadsheet. Results from the students research show a promising selection of new plant candidates for possible further testing. Over a two year period, more than 5000 South Florida students, ages 11 to 18, participated in GBE. Evaluation of the program shows an increased knowledge of and interest in science and science careers among students. The program has also boosted the demand for summer high school internships at FTBG, further developing expertise in plant research and science related to space exploration. Supported by a grant from NASA (NNX16AM32G) to Fairchild Tropical Botanic Garden.

Veggie↗

Plant Water Management (PWM)

The NASA Plant Water Management technology demonstration exploits recent advances in microgravity capillary fluidics to develop techniques for plant growth in a space environment. Plant growth in the low-g environment is often hampered by inadequate aeration and over-saturation in the root zone. The present effort aims to make passive use of poorly wetting liquids (i.e., contaminated water) within unique system geometries that effectively replace the role of gravity in providing sufficient aeration and hydration for simulated plants. Several ISS demonstrations are in preparation for launch in 2019, including hydroponic and soil approaches. Supportive terrestrial and low-g drop tower tests are conducted to aid in experiment design via proof-of-concepts, limits of operation, system stability, and others. The implications to short and long term applications are discussed in relation to plant growth facilities for both near-term microgravity plant science research and long duration human exploration missions.

Hatch, Tyler↗

Persistence of a Potential Pathogen on a Plant Microbiome-Does Seed Sanitization Matter?

Microbial interactions on, in, and around the seeds can have profound effects on plant growth, development, and productivity. These interactions can be casual or intimate in nature, but ultimately they all contribute in varying degrees to an ever-evolving microbiome. Plant microbiomes have been investigated over decades and new data continue to reveal how microbiomes play an important role in the plant’s success. Many eubacteria and fungi have been found to have a symbiotic relationship with plants and other microorganisms. The source of the plant microbiome on adult plants is provided by the seed via vertical transmission to leaf, root, flowers or fruit, as well as the surrounding environment.

Anirudha R. Dixit↗

Veggie on Ice: The Effects of Plant Production on Human Behavioral Health Long-Duration Antarctic Overwintering Missions

Exploration class missions pose an increased risk of adverse crew behavioral health and psychiatric conditions. Identifying and developing effective and efficient countermeasure strategies are necessary to maintain crew health and performance. Previous studies (VEG-04A and VEG-04B) in the Veggie vegetable-production system on the International Space Station have evaluated the behavioral health effects of caring for plants as a potential countermeasure for crew stresses in spaceflight. The presence of plants can augment sensory stimulation (visual, tactile, and olfactory), provide a psychological link to Earth, reduce stress, and increase happiness. However, further research is needed to understand this strategy for longer-duration missions in isolated, confined, extreme (ICE) environments. The Neumayer III Station in Antarctica is one such environment that hosts a small crew for fourteen-month missions, of which eight to nine months are the remote overwintering phase. Neumayer III also features EDEN ISS, a greenhouse facility with 12.5 m2 plant cultivation space that, like Veggie, currently supplements the crew diet with a variety of fresh produce. Up to nine crew members of the 2021 overwintering mission will complete behavioral health surveys that are designed to capture enjoyment and time spent conducting various plant-related activities, sensory stimulation, and active and passive psychological effects of having live plants and available fresh food in an ICE environment. Data will be collected once pre-mission, up to ten times in-mission at monthly increments, and once post-mission. This study aims to quantify the impact of plant care as a countermeasure strategy for human spaceflight and Antarctic exploration missions.

J M Bunchek↗