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At least 199 records · Page 11

Culture-Based Environmental Microbiology Monitoring of Crop-Based Space Food Systems (veggie Monitoring)

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. Quarterly monitoring of the microorganisms in the International Space Station (ISS) environment supports crew safety and contributes to a large set of microbial concentration and diversity data from air, surfaces and water samples. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Longer exploration missions may require spaceflight-based systems for growth of plants, and this investigation is expected to provide additional data to help establish requirements to protect these systems, plants, and crew, mitigating adverse microbial exposure.

Hamilton, Tanner↗

Microbiological Cleanliness of the Mars Pathfinder Spacecraft

The recently launched Mars Pathfinder spacecraft, involving a lander and a rover scheduled to land on Mars in July 1997, was required to comply with the National Aeronautics and Space Administration planetary protection regulations for such space missions.

Mars↗

Clean is not Sterile: A Planetary Science and Planetary Protection Perspective on Cleanroom Microbiology at NASA

The Astromaterials Acquisition and Curation Office at NASA is responsible for the curation of extraterrestrial samples from NASA’s past and future sample return missions. Our office curates samples from the moon, meteorites, comets, asteroids, cosmic dust and solar wind particles. All these samples are kept in cleanrooms to limit particulate and trace metal contamination, but none of these cleanrooms are specifically designed to control microbial contamination. During the early Apollo missions NASA scientists were very concerned with protecting the Earth from potential microbial contamination from the moon and with protecting the lunar samples from terrestrial microbes. NASA developed specialized equipment and clean rooms to keep these collections pristine. However, as we learned more about the lunar environment our concerns about microbial contamination lessened. Today none of the existing collections have microbial contamination requirements because they are not considered susceptible to microbial alteration under curation conditions (e.g. solar wind samples, and lunar samples) or have already been contaminated by terrestrial biology (meteorites collected in Antarctica). However, NASA’s OSIRIS-REx mission will land in 2023 with samples from a carbon rich asteroid that will be susceptible to microbial alteration. The Perseverance rover on Mars will begin to collect and cache samples that will be returned to Earth as soon as 2031. Martian samples may contain signs of extraterrestrial life and will have to be treated like the early Apollo samples. Martian samples will be isolated to protect the Earth, and must also be protected from terrestrial contamination. I will present microbial monitoring data from existing NASA cleanrooms and discuss how NASA is planning to use techniques from the pharmaceutical industry and academia to design new laboratories and equipment that will protect astromaterials and the earth from unwanted microbial contamination. I will also discuss a project to sample the external microbiome of the International Space Station. Results from this research will be used to design facilities for use on Mars that limit the amount of contamination associated with crewed missions.

Aaron B Regberg↗

Continued Environmental Microbiology Monitoring of the International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. To ensure occupants’ health and safety during their spaceflight residency, Environmental Health System (EHS) microbial samples including air, surface, and water, are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. Based upon data historically collected over the years, in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Microbial surface samples collected from the Veggie plant production system will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie locker, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond. This presentation will include a review of the study procedures and evaluations of the current results.

Christian Mena↗

Continued Environmental Microbiology Monitoring of The International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

The International Space Station is a closed environment where rotating sets of Crewmembers live and work. This environment is monitored to ensure occupants’ health and safety during their spaceflight residency by routine Environmental Health System (EHS) collection of microbial samples including air, surface, and water. The microbial samples are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. The current in-inflight microbial requirements were developed using this historical data collected by the routine environmental monitoring. These in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational EHS sampling by collecting additional microbial samples from the surface of the Veggie plant production system on ISS. These samples will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie system, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond.

Christian Mena↗

Developing a Tool to Collect Microbiological Samples in Space

We developed and tested a tool to collect aseptic samples during extravehicular activity or space walks. This tool can withstand the extreme temperature (-92 – 90 ˚C) and pressure variations without compromising the sterility of the collection device or the integrity of the sample. Results from ground testing the tool demonstrate that external surfaces of space suits are not sterile and that a wide variety of human associated bacteria and some fungi can survive at least seven hours of exposure to vacuum. These bacteria include non-spore forming organisms like Staphylococcus that are not normally considered extremophiles We plan to use this tool to characterize the exterior of the International Space Station. We expect the results of this work to guide the planetary protection requirements and contamination control strategy for crewed missions to Mars. This tool could also be used to monitor contamination associated with the Artemis missions to the moon or collect aseptic samples robotically in environments that are not suitable for human exploration.

A B Regberg↗