Results for “Lettuce” — Page 11

NASA NTRS2017

Space Plants for Astronaut Consumption

Growing plants in space will be an essential part of sustaining astronauts during long-range missions. During the summer of 2017, three female NASA interns, have been engaged in research relevant to food production in space, and will present their projects to an all female program known as Girls in STEM camp. Ayla Grandpre, a senior from Rocky Mountain College, has performed data mining and analysis of crop growth results gathered through Fairchild Botanical Gardens program, Growing Beyond Earth. Ninety plants were downselected to three for testing in controlled environment chambers at KSC. Ayla has also managed an experiment testing a modified hydroponics known as PONDS, to grow mizuna mustard greens and red robin cherry tomatoes. Emma Boehm, a senior from the University of Minnesota, has investigated methods to sterilize seeds and analyzed the most common microbial communities on seed surfaces. She has tested a bleach fuming method and an ethanol treatment. Emma has also tested Tokyo bekana Chinese cabbage seeds from four commercial seed vendors to identity differences in germination and growth variability. Lastly, Payton Barnwell, a junior from Florida Polytechnic University has shown that light recipes provided by LEDs can alter the growth and nutrition of 'Outredgeous' lettuce, Chinese cabbage, and Mizuna. The results of her light quality experiments will provide light recipe recommendations for space crops that grown in the Advanced Plant Habitat currently aboard the International Space Station.

VEGGIE↗

NASA NTRS2018

New Crop Selection

For extended space flight, reliable food supplies are a necessity. Most of the food products consumed by astronauts today are stored for flight via freeze drying. Fresh food is needed to supplement known national deficiencies in the stored food diet (Cooper et al.). This is so because stored foods can lose nutritional value. Fresh food is the answer to the nutritional demands of space flight. Kennedy Space Center's Utilization and Life Sciences Office (UB-A), under the Exploration Research and Technology Program (UB), conducts research on plant growth and development under International Space Station (ISS) conditions. UB-A analyzes the growth responses of leafy greens in microgravity and through the manipulation of environmental conditions (CO2 levels, light intensity, relative humidity, and water delivery). By manipulating growing conditions researchers can optimize food production using minimal/restricted resources. The New Crop Selection experiments are testing the suitability of leafy crops to ISS conditions. Results from this study showed that 'Dragoon' Lettuce and 'Red Russian' Kale have the largest fresh mass.

Bhuiyan, Ruqayah H.↗

NASA NTRS2018

A Microbial Analysis of Space-Grown Produce

Before space crops become a permanent staple of the astronaut diet, we must first understand how plants and pathogenic microbes interact in microgravity. Crops such as red romaine lettuce and Chinese cabbage were grown on the International Space Station and sent back to Kennedy Space Center for microbial analysis. Aerobic plate counts and metagenomic sequencing were used to characterize bacterial and fungal communities for plants and their respective ''pillows''. These data will be used to create new guidelines for the microbial safety of space-grown produce, and will help us better protect astronauts from food-borne pathogens like E.coli, Staphylococcus, and Salmonella.

Barash, Eric L.↗

NASA NTRS2018

Baseline Microbial Assessment of Fresh Produce

Currently no standards or requirements exist for microbial food safety for space grown produce (fresh plant foods). Without standards it is difficult to assess produce handling and sanitization options for the ISS and future exploration missions. We are conducting a literature review of microbial levels on fresh food and then carrying out measurements (microbial counts) of grocery store purchased and controlled environment-grown crops. Testing will include lettuce, mizuna, cherry tomato, pepper, and radish, all candidate crops for pick-and-eat testing on ISS and near term exploration missions. Growth chamber conditions will be set to mimic an ISS or spacecraft environment. Assays will include specific pathogens (Enterobacteriacea, Salmonella sp., and Aspergillus flavus) and total culturable microorganisms using aerobic plate counts, and total yeast and mold counts. Analyses will follow the FDA Bacteriological Analytical Manual methods. The goal of the project is to establish a baseline for expected microbial levels found on fresh plant foods that might be grown on ISS and near term missions, and develop risk assessment and microbial safety recommendations for these types of fresh foods.

Hummerick, M. E.↗

NASA NTRS2018

Plant Growth Research for Future Food Production - Seed Microbiome Project

Humanity is currently on the precipice of a new era: one where human civilization is no longer bound to the confines of a single planet. Now, organizations like the National Aeronautics and Space Administration (NASA) have extended their areas of research and are beginning to focus not just on getting humans into space, but also to keep them safe, healthy, and sane. This focus falls under the Human Research Program (HRP). A focus of this program is "dedicated to discovering the best methods and technologies to support safe, productive human space travel" [1]. For "safe and productive space travel" to occur, astronauts must have proper nutrition [1]. While sending up large amounts of dried and packaged food with every shuttle might work fairly well in the short term, when only a few people need to be fed, it will not be sustainable, especially as NASA looks toward longer space journeys beyond the Earth's orbit. Research into this area falls under Advanced Life Support (ALS), whose mission is to develop regenerative life support systems to support future NASA long-duration missions [2]. This would involve growing crops in space to supplement astronaut diets [2]. An important, yet often overlooked, part of growing crops in any environment is the microbial organisms that inhabit the plants' microbiomes. The Seed Microbiome Project aims to investigate the microbial presence throughout the life stages of three crops, Mizuna Mustard, 'Outredgeous' Red Romaine Lettuce, and 'Red Robin' Tomato, that have either been or will be grown on the ISS.

Birtell, Eva↗

NASA NTRS2019

Does Seed Sanitization Affect the Plant Rhizosphere Microbiome and Its Ability to Compete with the Human Associated Pathogen, E. coli on Salad Crops?

Cultivation of crops in controlled environmental agricultural systems may limit microbial colonization and reduce diversity of the microbial communities. Practices like seed and growth medium sanitization may further impact microbial communities in the mature plant and the plant’s capacity to limit the growth of pathogens through competition. As humans expand their travels to space, understanding plant growth, health, and development in closed environments will be critical to the success of producing a safe, supplemental food source for astronauts. To determine the persistence of a potential human pathogen in plant growth and development, sanitized and unsanitized seeds from, mizuna (Brassica rapa var japonica) and red romaine lettuce (Lactuca sativa cultivar ‘Outredgeous’), were inoculated with Escherichia coli, ATCC 21445, germinated under simulated International Space Station (ISS) environmental conditions and harvested every 7 days until maturity. The persistence of E. coli in the rhizosphere was determined by plating on selective media, real time PCR (Polymerase Chain Reaction) and community sequencing of the rhizosphere communities. E. coli was detected in the crops’ roots and leaves for several weeks post germination. At day 28, plants from sanitized seeds had significantly higher counts of E. coli on the roots than those from unsanitized seeds. E. coli was also detected on a few uninoculated plants indicating airborne cross contamination among plants in the same growth chamber and suggesting an influence of the natural microbiome on human pathogen survival and persistence in leafy greens. Sequencing analysis revealed variations in composition and diversity between the communities. Understanding the microbial community of the rhizospheric microbiome is only the first step in determining the relationships between plants. Additional studies to include genotypic and phenotypic variations in the plants should be considered to determine if the natural microbes in the rhizosphere may contribute to the health and therefore, safety of the edible plants.

Khodadad, Christina L. M.↗

NASA NTRS2019

How Does Water Delivery System Design Impact the Microbial Load of Salad Crops?

In a microgravity setting, such as the environment aboard the International Space Station (ISS), an ideal plant water delivery system is one that can grow edible crops with minimal resource consumption and minimal risk to crew members. There are also concerns associated with the ability to control fluid escape and biofilm formation resulting in potential dangers to systems, crops, or crewmembers. To identify an appropriate system, candidate systems were assembled and operated under simulated ISS environmental conditions (T,CO2,and RH) with red romaine lettuce (Lactuca sativa cultivar 'Outredgeous') as a model crop. Fluid reservoirs and randomly selected planting sites were sampled every seven days until maturity at which point edible plant biomass and root samples were also taken. Heterotrophic bacteria and fungi growth patterns throughout each planting cycle were determined by plate counts on appropriate agar media. The candidate systems were compared to a classic hydroponics system as a control and harvested crops were compared to controls as well as Veggie-grown and market produce. Plants harvested from candidate systems yielded lower average heterotrophic bacteria and fungi per gram of plant mass levels when compared to market and Veggie samples as well as those from the control system. Additional studies to evaluate the system sanitation regimen as well as testing additional crops should be considered to aid in the selection of an ideal system.

Curry, Aaron B.↗

NASA NTRSDate not supplied

Exposure of Plant Seeds to the Space Environment outside the International Space Station

If crop seeds are to be stored on space vehicles for long-duration missions, the effects of the space environment on the seeds must be understood for developing mitigation strategies to prevent seed degradation. The MISSE-Seed project was designed to investigate the effects of space exposure on seed quality and storage. The project also tested the Materials International Space Station Experiment (MISSE) platform for exposing biological samples to the space environment and evaluated the capability of a newly designed passive sample containment vessel for the provision of acceptable storage conditions for seeds, or other biological samples, to be preserved upon exposure to the space environment outside the ISS. The experiment was launched to the ISS on NG-15and returned to Earth aboard SpX-24. The specimens consisted of eleven seed varieties (Lettuce, Scarlet Frills, Amara, Garnet Giant, Pac Choi, Radish, Mizuna, Tomato, Cauliflower, Pepper, and Arabidopsis). The exposure lasted eight months outside the ISS in the MISSE hardware at the Zenith position. Data-loggers and thermoluminescent dosimeters (TLDs) were included in each container to record the environmental data. Temperature profiles and radiation/UV exposure data were also provided by Aegis Aerospace, the MISSE Implementation Partner. In this presentation, the hardware and experimental design, the environmental profiles, and the results of post-flight germination tests will be presented.

Jeffrey T Richards↗

NASA NTRSDate not supplied

Exposure of Plant Seeds to the Space Environment Outside the International Space Station

If crop seeds are to be stored on space vehicles for long-duration missions, the effects of the space environment on the seeds must be understood for developing mitigation strategies to prevent seed degradation. The MISSE-Seed project was designed to investigate the effects of space exposure on seed quality and storage. The project also tested the Materials International Space Station Experiment (MISSE) platform for exposing biological samples to the space environment and evaluated the capability of a newly designed passive sample containment vessel for the provision of acceptable storage conditions for seeds, or other biological samples, to be preserved upon exposure to the space environment outside the ISS. The experiment was launched to the ISS on NG-15and returned to Earth aboard SpX-24. The specimens consisted of eleven seed varieties (Lettuce, Scarlet Frills, Amara, Garnet Giant, Pac Choi, Radish, Mizuna, Tomato, Cauliflower, Pepper, and Arabidopsis). The exposure lasted eight months outside the ISS in the MISSE hardware at the Zenith position. Data-loggers and thermoluminescent dosimeters (TLDs) were included in each container to record the environmental data. Temperature profiles and radiation/UV exposure data were also provided by Aegis Aerospace, the MISSE Implementation Partner. In this presentation, the hardware and experimental design, the environmental profiles, and the results of post-flight germination tests will be presented.

Jeffrey T Richards↗

NASA NTRSDate not supplied

3D Printed Materials Characterization for Rapid Prototyping and Plant Growth

Space Biology experimentation but their effects during plant growth and sanitation have not been documented. With further testing we can determine how certain 3D printed materials affect our plants. The way we tested germination included laying two pieces of germination paper down with the printed petri dish insert of the various material and 14 seeds of Outrageous lettuce to fit in the holes of the insert. We kept an even moisture in all parts by wrapping the petri dishes in two layers of parafilm tape. This project tests over 15 different materials to document performance during plant growth scenarios. This research will not only help space biology but also citizens who use these materials to understand the potential of leaching and ill effects. 3D printing is a recent generally accessed technology with new materials coming out every day. It is important to understand how materials could impact the way the plants grow, and the safety of the food grown. As we continue testing these materials, we will also test engineering properties like tension and elasticity. We have a microbiology team working on this project as well to test biofilm formation. From the germination tests we have completed we have seen little impact on germination rate of these seeds. However, some parts absorb moisture. An ideal material is one that doesn’t leach chemicals or microplastics, can withstand high tension and compression, and doesn’t promote microbial growth. This work is funded by NASA. M. Gandhi was funded by MAIANSE.

Mily Gandhi↗

NASA NTRSDate not supplied

Exposure of Plant Seeds to the Space Environment Outside the International Space Station

Crop seeds may prove to be a vital component of future long-term spaceflight missions, and the impacts of extended exposure on seed degradation is not well understood. The MISSE-Seed project was designed to investigate the effects of space exposure on seed quality and storage. The project also served as a test of biological materials aboard the Materials International Space Station Experiment (MISSE) platform, and evaluated the capability of a newly designed passive sample containment vessel for the provision of acceptable storage conditions for seed and other biological samples to be preserved upon exposure to the space environment outside the ISS. MISSE-Seed was launched to the ISS on NG-15 in July of 2021 and returned to Earth aboard SpX-24 after approximately 8 months of exposure to the space environment. The specimens consisted of eleven seed varieties (Lettuce, Scarlet Frills, Amara, Garnet Giant, Pac Choi, Radish, Mizuna, Tomato, Cauliflower, Pepper, and Arabidopsis). Data-loggers and thermoluminescent dosimeters (TLDs) were included in each container to record environmental data. Temperature profiles and radiation/UV exposure data were also provided by Aegis Aerospace, the MISSE Implementation Partner. Hardware and experimental design, environmental profiles, and post-flight germination and growth results will be presented.

Alexander D Meyers↗

NASA NTRS2015

Mars OASIS (Mars Operational Agricultural System for In-Situ Specialization) System Reference Manual

In order to enable long term habitation on planetary surfaces, a means of sustainable food production must be developed. Addressing this need for surface habitats on Mars, the MarsOASIS team has developed a concept for a Martian surface greenhouse for unmanned crop production research as a proof of concept for larger scale food production facilities for manned surface missions. Utilizing in-situ resources such as the Martian atmosphere, sunlight, and UV-C radiation, the greenhouse aims to provide a sustainable method of long-term food production requiring minimal consumable resources. The MarsOASIS system is capable of growing a full life cycle of Outredgeous lettuce with its autonomous control system designed for a unmanned environment, only requiring teleoperation in extreme circumstances. A reduced-scope prototype of MarsOASIS is being developed to test technologies such as a natural/artificial hybrid lighting system, a closed water recycling system, remote teleoperation, and fully autonomous monitoring and control of the greenhouse. The prototype is currently in the final stages of design, with a full demonstration of plant life cycle testing set to occur in summer 2015. Results from this prototype demonstration will help quantify the feasibility of the innovative approaches seen in the MarsOASIS design.

Asa Darnell↗

NASA NTRS2023

3D Printed Substrate

In preparation for long-term, manned, deep space missions, NASA requires a sustainable system for crop and food production. This system has a variety of benefits, including a fresh food supply, improvements in air quality, a lower need to resupply and psychological benefits for the gardeners. Specifically, NASA is attempting to improve and iterate their Vegetable Production System (VEGGIE), a plant growth unit currently aboard the International Space Station (ISS). The VEGGIE unit is highly dependent on a variety of factors, including passive water delivery, growth lights, rooting pillows and cabin conditions. While previous teams have improved and redesigned water reservoirs, the primary objective of the project was to create a new substrate unit to replace the current rooting pillow design. The current design consists of an electrostatic bag filled with arcillite and a slow-release fertilizer pellet. The prototype design retains the fertilizer pellet for nutrient consistency, however the outer bag and arcillite fillings have been replaced with a 3D printed lattice block. This lattice block has several key points that allow it to function in similar ways to the current design: 1. The lattice planes are porous, with each pore offset such that the overall porosity is the same as the arcillite filling. 2. The block demonstrates a wicking nature and is able to pull water upwards towards the roots. This minimizes time needed to integrate with the existing water reservoir. 3. The filament used is highly flexible and is able to pull apart to accommodate root growth. 4. The lattice planes are connected by microfibers left behind from the printing process. These fibers provide support for the growing roots and keep the lattice planes properly aligned. The substrate block design seeks to reduce the payload costs by being entirely 3D printed. While filament would still need to be provided to the ISS, it is far less expensive than shipping arcillite due to the significantly lower weight. The final design has iterated the lattice plane concept and utilizes vertically placed planes with pores running parallel to the water reservoir. This design has reliably shown water uptake and retention and has been successful in growing multiple romaine lettuce plants. Future work should include further growth testing using multiple plant species, compost and reusability testing, food safety testing and microgravity growth testing.

Affan Bhutta↗

NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Space Environments

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesized to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

Luke Leslie Fountain↗

NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Spaceflight Cabin Environments

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesised to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

Luke Fountain↗

NASA NTRSDate not supplied

Understanding Plant Nitrogen Form Preference Responses to Elevated CO2 Earth and Spaceflight Cabin Environments.

Future long-duration missions to the International Space Station (ISS) and beyond will require a sustainable supply of food to support human crews. The spaceflight cabin environment often contains very high concentrations of CO2, and it is therefore crucial to understand plant responses to elevated CO2 (eCO2) environments. Much focus has been given to plant photosynthetic and performance parameters in response to eCO2, but studies on the effects of eCO2 on nitrogen uptake are poorly understood. Our previous work at The University of Sheffield, UK using novel stable isotope approaches has shown enhanced ammonium uptake and preference compared to nitrate at eCO2 in varieties of spring barley, hypothesised to be an indirect consequence of changes in photosynthesis and photorespiration. However, several varieties did not display altered preference under eCO2 and the universality of this response remains to be understood. In this NASA NPP project, several candidate crop species such as lettuce, radish and tomato will be screened using stable isotopes to assess whether N preference changes in favor of ammonium in response to eCO2 and whether this remains true at super-elevated CO2 (seCO2), which is often experienced on the ISS. Photosynthetic and related measurements will help to disentangle the relationship between these responses and photosynthesis. This work will enable the development of optimized nutrient regimes for candidate crops in space and in future Lunar and Martian habitats and will pave the way for selection of crop varieties adapted to an eCO2 and/or seCO2 environment. Moreover, this research will further our understanding of plant responses to the eCO2 environment brought about by climate change, allowing the development of future-proof crops that will help to maintain food security. This NPP Fellowship is funded by NASA Space Biology.

space plant biology↗

NASA NTRSDate not supplied

Nutrition of Antarctic-grown Crops to Supplement the Crew Diet, with Applications for Spaceflight

The availability of fresh produce during longer-duration spaceflight missions is being explored as a countermeasure for human biobehavioral health and performance, including how fresh fruits and vegetables can supplement the crew diet with nutrients that are predicted to become deficient throughout the missions. Crop production in the Veggie and Advanced Plant Habitat vegetable production chambers on the International Space Station (ISS) have tested pick-and-eat crops in spaceflight. However, the limited plant cultivation volume of these chambers has restricted the sample sizes and biomass allocated for nutrition assessment. To gain a better understanding of the nutrient composition of pick-and-eat crops, leafy greens and fruiting plants were grown in the EDEN ISS plant cultivation facility near the German Neumayer Station III in Antarctica from March 2021 to January 2022. Target crops for nutrition sampling aligned with cultivars grown in spaceflight, including ‘Outredgeous’ red romaine lettuce, Mizuna mustard, ‘Red Robin’ dwarf cherry tomato, and NuMex ‘Española Improved’ chili pepper. Plants subsamples were taken at harvest; in the event of multiple harvests from the same plants, subsamples were taken at both the first and final harvests. ‘Outredgeous’ and Mizuna were also grown multiple times throughout the year, allowing multiple grow-outs to be analyzed for potential effects across the growing season. Subsamples were first weighed for fresh mass, dried in a dedicated oven at 70°C for 96 h, weighed again, and stored in air-tight containers inside Neumayer Station III. At the beginning of 2022, the samples were shipped to NASA’s Kennedy Space Center, where they are currently being analyzed with ion chromatography for nutrients of interest in the astronaut diet, including calcium, iron, magnesium, and potassium. This study aims to increase our understanding of how the crops that have successfully grown on the ISS can supplement the crew diet, as well as how other factors like plant age and the number of days the facility has been in operation may impact nutrient concentrations. Such findings can improve the crop selection process and how pick-and-eat crops are cultivated during spaceflight missions.

Jess M. Bunchek↗

NASA NTRSDate not supplied

Nutrition of Antarctic-grown Crops to Supplement the Crew Diet, with Applications for Spaceflight

The availability of fresh produce during longer-duration spaceflight missions is being explored as a countermeasure for human biobehavioral health and performance, including how fresh fruits and vegetables can supplement the crew diet with nutrients that are predicted to become deficient throughout the missions. Crop production in the Veggie and Advanced Plant Habitat vegetable production chambers on the International Space Station (ISS) have tested pick-and-eat crops in spaceflight. However, the limited plant cultivation volume of these chambers has restricted the sample sizes and biomass allocated for nutrition assessment. To gain a better understanding of the nutrient composition of pick-and-eat crops, leafy greens and fruiting plants were grown in the EDEN ISS plant cultivation facility near the German Neumayer Station III in Antarctica from March 2021 to January 2022. Target crops for nutrition sampling aligned with cultivars grown in spaceflight, including ‘Outredgeous’ red romaine lettuce, Mizuna mustard, ‘Red Robin’ dwarf cherry tomato, and NuMex ‘Española Improved’ chili pepper. Plants subsamples were taken at harvest; in the event of multiple harvests from the same plants, subsamples were taken at both the first and final harvests. ‘Outredgeous’ and Mizuna were also grown multiple times throughout the year, allowing multiple grow-outs to be analyzed for potential effects across the growing season. Subsamples were first weighed for fresh mass, dried in a dedicated oven at 70°C for 96 h, weighed again, and stored in air-tight containers inside Neumayer Station III. At the beginning of 2022, the samples were shipped to NASA’s Kennedy Space Center, where they are currently being analyzed with ion chromatography for nutrients of interest in the astronaut diet, including calcium, iron, magnesium, and potassium. This study aims to increase our understanding of how the crops that have successfully grown on the ISS can supplement the crew diet, as well as how other factors like plant age and the number of days the facility has been in operation may impact nutrient concentrations. Such findings can improve the crop selection process and how pick-and-eat crops are cultivated during spaceflight missions.

Jess M. Bunchek↗