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At least 199 records · Page 11
All sourcesNASA NTRSDOE OSTI
NASA NTRS1996

Vitamin D endocrine system after short-term space flight

The exposure of the body to microgravity during space flight causes a series of well-documented changes in Ca(2+) metabolism, yet the cellular/molecular mechanisms leading to these changes are poorly understood. There is some evidence for microgravity-induced alterations in the vitamin D endocrine system, which is known to be primarily involved in the regulation of Ca(2+) metabolism. Vitamin D-dependent Ca(2+) binding proteins, or calbindins, are believed to have a significant role in maintaining cellular Ca(2+) homeostasis. We used immunocytochemical, biochemical and molecular approaches to analyze the expression of calbindin-D(sub 28k) and calbindin-D(sub 9k) in kidneys and intestines of rats flown for 9 days aboard the Spacelab 3 mission. The effects of microgravity on calbindins in rats in space vs. 'grounded' animals (synchronous Animal Enclosure Module controls and tail suspension controls) were compared. Exposure to microgravity resulted in a significant decrease in calbindin-D(sub 28k) content in kidneys and calbindin-D(sub 9k) in the intestine of flight and suspended animals, as measured by enzyme-linked immunosorbent assay (ELISA). Immunocytochemistry (ICC) in combination with quantitative computer image analysis was used to measure in situ the expression of calbindins in kidneys and intestine, and insulin in pancreas. There was a large decrease in the distal tubular cell-associated calbindin-D(sub 28k) and absorptive cell-associated calbindin-D(sub 9k) immunoreactivity in the space and suspension kidneys and intestine, as compared with matched ground controls. No consistent differences in pancreatic insulin immunoreactivity between space, suspension and ground controls was observed. There were significant correlations between results by quantitative ICC and ELISA. Western blot analysis showed no consistent changes in the low levels of intestinal and renal vitamin D receptors. These findings suggest that a decreased expression of calbindins after a short-term exposure to microgravity and modelled weightlessness, may affect cellular Ca(2+) homeostasis and contribute to Ca(2+) and bone metabolism disorders induced by space flight.

Rhoten, William B.↗
NASA NTRS1997

Glacier Bay, Alaska, from the Ground, Air, and Space

This tape uses a combination of video, three-dimensional computer imaging, and still photographs to provide a descriptive overview of the life-cycle and environmental effects of glaciers. An historical prospective of researchers and the contribution that they have made to the understanding of glaciers and Glacier Bay is presented. The data collected from these scientists have been documented and used by means of scientific visualization in the hope of learning how glacial activity relates to climate changes.

Hall, Dorothy K.↗
NASA NTRS1997

Planetary Aeronomy and Related Studies

Mercury atmosphere - Sprague and Hunten, in collaboration with Katharina Lodders of Washington University, proposed, mainly on cosmochemical grounds, that S atoms are an important constituent of the atmosphere (30 times more abundant than sodium). This paper has appeared in Icarus. We also suggest that condensed sulfur is an excellent candidate for the radar-bright polar caps, more plausible than water ice because the latter is only barely stable even in permanently-shadowed craters. The best prospect for detection of the vapor is through its resonance lines, a triplet near 1814 A. Mercury is too close to the Sun to be observed by any existing space telescope, but there is some prospect that the search could be made from a Shuttle-based spectrograph such as Lyle Broadfoot's USTAR. Sprague and Hunten have completed an elaborate data analysis of over 100 measurements of the Na D lines, obtained with the 61-inch telescope and our echelle spectrograph. Full account has been taken of the radiative-transfer problem that arises because the Na atmosphere is not optically thin. The output of this code is used in another program that makes an elaborate inverse interpolation in two angles and optical depth and computes the effect of the seeing (always bad for Mercury). The seeing is determined by fitting cuts across a computed image to part of the spectrum adjacent to the sodium lines, and typically ranges from slightly less than 4 arcsec to worse than 6 (diameter at l/e of a Gaussian). The final result is a list of Na abundances, with some information on spatial distribution. One particularly interesting result of further analysis is a strong abundance maximum in the morning relative to the afternoon, confirming an earlier result for potassium, based on much fewer measurements. The analysis are completed during the extension of the present grant. This work depends heavily on the Hapke parameters used to estimate the reflectance of Mercury's surface. The paper by Domingue et al. examines the credibility of the available parameters, which are derived from disk-unresolved photometry, and concludes that errors in the derived Na abundances could be as great as 30%.

Hunten, D. M.↗
NASA NTRS1985

Visualization of Coronary Arteries from Intravenous Angiograms

Under most circumstances, the coronary arteries are not satisfactorily visualized in intravenous angiograms. The objective of this study is to develop computer image enhancement methods that will improve the quality of the latent coronary images to a degree sufficient to detect an obstructive lesion. Such a technique, if successful, could be used as a first step alternative to conventional coronary angiography for individuals with ambiguous noninvasive cardiac tests. The determination of no lesion from the intravenous procedure would relieve the need for the conventional angiogram, while verification of an obstructive lesion could be followed by a conventional angiogram. The nature of the imaging problem and a description of the methods and initial processing results are described in this paper.

Selzer, Robert H.↗
NASA NTRS1995

Gravity Sensors and the Role of 3-D Visualization and Simulation in Biomedical Research

Ross 3.0 software was developed in the Biocomputation Center for semi-automated reconstruction of objects from serial thin sections. Data are captured directly from a transmission electron microscope via a video camera to a graphics workstation where the sections are mosaicked and contours are traced, registered and displayed by semi-automated methods. For the first time, macular type II cells are described completely for their innervation patterns. The purposes are to learn more about the fundamental circuitry of the macula and to demonstrate whether the terminals are altered morphologically by space flight. Current examples, from the medial part of the macula, are from maculas collected in-flight on the Space Life Sciences-2 mission, 4.5 hrs post-flight, and from a ground control. Results show that the typical type 11 cell receives processes from up to six nearby calyces or afferents. Nearly all the processes are elongated; some have bouton-like swellings and numerous vesicles. Multiple (2 to 4) processes from a single calyx to a type II cell are common, and approx. 1/3 of the processes innervate 2 type II cells of a neighboring cluster of 3 cells. About 2% of type II cells resemble type I cells morphologically and are surrounded by demicalyces. Differences in size or shape of the terminals under flight conditions could not be determined because the sample size is still too small; but it is clear that reconstruction methods provide insights into macular circuitry not obtainable by other techniques. The results demonstrate a morphological basis for interactions between adjacent receptive fields, through feedback-feedforward connections, during preprocessing of linear acceleratory information by the maculas. While the methods are currently being tested using vestibular maculas as the model system, it is clear that the technology is applicable to any tissue that can be physically or optically sectioned. ROSS software has already been implemented for reconstructing objects from tissues studied by confocal and by transmitted light microscopy, and research into magnetic resonance imaging-computational tomography combined visualization are underway.

Ross, Muriel D.↗
NASA NTRS2003

Microstructure and Properties of Thermally Sprayed Functionally Graded Coatings for Polymeric Substrates

The use of polymer matrix composites (PMCs) in the gas flow path of advanced turbine engines offers significant benefits for aircraft engine performance but their useful lifetime is limited by their poor erosion resistance. High velocity oxy-fuel (HVOF) sprayed polymer/cermet functionally graded (FGM) coatings are being investigated as a method to address this technology gap by providing erosion and oxidation protection to polymer matrix composites. The FGM coating structures are based on a polyimide matrix filled with varying volume fractions of WC-Co. The graded coating architecture was produced using a combination of internal and external feedstock injection, via two computer-controlled powder feeders and controlled substrate preheating. Porosity, coating thickness and volume fraction of the WC-Co filler retained in the coatings were determined using standard metallographic techniques and computer image analysis. The pull-off strength (often referred to as the adhesive strength) of the coatings was evaluated according to the ASTM D 4541 standard test method, which measured the greatest normal tensile force that the coating could withstand. Adhesive/cohesive strengths were determined for three different types of coating structures and compared based on the maximum indicated load and the surface area loaded. The nature and locus of the fractures were characterized according to the percent of adhesive and/or cohesive failure, and the tested interfaces and layers involved were analyzed by Scanning Electron Microscopy.

Ivosevic, M.↗
NASA NTRS1996

Calbindins decreased after space flight

Exposure of the body to microgravity during space flight causes a series of well-documented changes in Ca2+ metabolism, yet the cellular and molecular mechanisms leading to these changes are poorly understood. Calbindins, vitamin D-dependent Ca2+ binding proteins, are believed to have a significant role in maintaining cellular Ca2+ homeostasis. In this study, we used biochemical and immunocytochemical approaches to analyze the expression of calbindin-D28k and calbindin-D9k in kidneys, small intestine, and pancreas of rats flown for 9 d aboard the space shuttle. The effects of microgravity on calbindins in rats from space were compared with synchronous Animal Enclosure Module controls, modeled weightlessness animals (tail suspension), and their controls. Exposure to microgravity resulted in a significant and sustained decrease in calbindin-D28k content in the kidney and calbindin-D9k in the small intestine of flight animals, as measured by enzyme-linked immunosorbent assay (ELISA). Modeled weightlessness animals exhibited a similar decrease in calbindins by ELISA. Immunocytochemistry (ICC) in combination with quantitative computer image analysis was used to measure in situ the expression of calbindins in the kidney and the small intestine, and the expression of insulin in pancreas. There was a large decrease of immunoreactivity in renal distal tubular cell-associated calbindin-D28k and in intestinal absorptive cell-associated calbindin-D9k of space flight and modeled weightlessness animals compared with matched controls. No consistent difference in pancreatic insulin immunoreactivity between space flight, modeled weightlessness, and controls was observed. Regression analysis of results obtained by quantitative ICC and ELISA for space flight, modeled weightlessness animals, and their controls demonstrated a significant correlation. These findings after a short-term exposure to microgravity or modeled weightlessness suggest that a decreased expression of calbindins may contribute to the disorders of Ca2+ metabolism induced by space flight.

Non-NASA Center↗
NASA NTRS1989

Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneurons

The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.

NASA Discipline Musculoskeletal↗
NASA NTRS2006

Acceleration of Stereo Correlation in Verilog

To speed up vision processing in low speed, low power devices, embedding FPGA hardware is becoming an effective way to add processing capability. FPGAs offer the ability to flexibly add parallel and/or deeply pipelined computation to embedded processors without adding significantly to the mass and power requirements of an embedded system. This paper will discuss the JPL stereo vision system, and describe how a portion of that system was accelerated by using custom FPGA hardware to process the computationally intensive portions of JPL stereo. The architecture described takes full advantage of the ability of an FPGA to use many small computation elements in parallel. This resulted in a 16 times speedup in real hardware over using a simple linear processor to compute image correlation and disparity.

machine vision↗
NASA NTRS2010

Modeling Regular Replacement for String Constraint Solving

Bugs in user input sanitation of software systems often lead to vulnerabilities. Among them many are caused by improper use of regular replacement. This paper presents a precise modeling of various semantics of regular substitution, such as the declarative, finite, greedy, and reluctant, using finite state transducers (FST). By projecting an FST to its input/output tapes, we are able to solve atomic string constraints, which can be applied to both the forward and backward image computation in model checking and symbolic execution of text processing programs. We report several interesting discoveries, e.g., certain fragments of the general problem can be handled using less expressive deterministic FST. A compact representation of FST is implemented in SUSHI, a string constraint solver. It is applied to detecting vulnerabilities in web applications

Fu, Xiang↗
NASA NTRS2019

Data Validation: Difference Imaging and Centroid Analysis

This Design Note describes the theory and application of difference imaging for automated validation of planet candidates in the Data Validation (DV) CSCI. This is one of the diagnostic tools employed in DV for planet candidate validation. The other DV diagnostics are described in separate KADNs and in the reference documents listed below. This document describes the difference image generation process, PRF-based centroiding of the out-of-transit and difference images, computation of quarterly centroid offsets and associated uncertainties, and determination of robust weighted mean centroid offsets/uncertainties over all available quarterly data sets. PRF-based centroiding is performed with calls to a library of functions that is shared with thePhotometric Analysis (PA) and Photometer Data Quality (PDQ) CSCIs. The centroiding library will be the subject of a separate Design Note.

Twicken, Joseph↗
NASA NTRS2020

Using X-Ray Computed Tomography to Image Apollo Drive Tube 73002

The Apollo missions collected 382 kg of rock, regolith, and core samples from six locations on the nearside of the Moon. Today, just over 84% by mass of the Apollo collection remains in pristine condition within the curation facility at Johnson Space Center. Most Apollo samples have been well characterized, however there are several types of samples that have remained wholly or largely unstudied since their return, and/or that have been curated under special conditions. These sample types are: (1) unopened samples sealed under vacuum on the Moon; (2) unopened (but unsealed) drive tubes; (3) Apollo 17 samples frozen shortly after their return; and (4) Apollo 15 samples opened and stored in a helium atmosphere since their return. Last summer, NASA solicited proposals for the Apollo Next Generation Sample Analysis Program (ANGSA), and 9 teams were selected to study: (1) unsealed, unopened drive tube 73002; (2) sealed, unopened drive tube 73001 (paired with 73002); and (3) a subset of the frozen and He-purged samples [1]. The first sample opened as part of the ANGSA program was drive tube 73002. This is a 30 cm long, 4 cm diameter drive tube collected on a landslide deposit near Lara Crater at the Apollo 17 landing site. It was part of a 60 cm long double drive tube collected, and the bottom half of the tube (73001) was sealed under vacuum on the Moon [2]. Prior to opening sample 73002, the sample was imaged with a high resolution Xray Computed Tomography (XCT) scan of the entire tube. Additional XCT scans have been made of “large” clasts removed from the core as part of the dissection process [3]. Here we present a first look at the XCT data from 73002, and talk about the utility of the scans as part of the curation process, including the potential for future science returns from the high resolutions scans.

Zeigler, R. A.↗
NASA NTRSDate not supplied

Development of a Computer Tool to Analyze MISSE Images

The Materials International Space Station Experiment (MISSE) contains individual experiments focused on the investigation of the effects that occur because of the exposure of the material to the space environment. The specimens are mounted on the exterior of the International Space Station (ISS) on the MISSE-Flight Facility (MISSE-FF) and are exposed to the extreme environmental conditions. There have been 14 MISSE missions since the establishment in 2001. This project will evaluate MISSE 9 - MISSE 13 specimens and how they change due to the space environment.

MISSE↗
NASA NTRS1988

Knowledge-based low-level image analysis for computer vision systems

Two algorithms for entry-level image analysis and preliminary segmentation are proposed which are flexible enough to incorporate local properties of the image. The first algorithm involves pyramid-based multiresolution processing and a strategy to define and use interlevel and intralevel link strengths. The second algorithm, which is designed for selected window processing, extracts regions adaptively using local histograms. The preliminary segmentation and a set of features are employed as the input to an efficient rule-based low-level analysis system, resulting in suboptimal meaningful segmentation.

Dhawan, Atam P.↗
NASA NTRS1983

Texture functions in image analysis: A computationally efficient solution

A computationally efficient means for calculating texture measurements from digital images by use of the co-occurrence technique is presented. The calculation of the statistical descriptors of image texture and a solution that circumvents the need for calculating and storing a co-occurrence matrix are discussed. The results show that existing efficient algorithms for calculating sums, sums of squares, and cross products can be used to compute complex co-occurrence relationships directly from the digital image input.

Cox, S. C.↗
NASA NTRS2009

Spatial Modulation Improves Performance in CTIS

Suitably formulated spatial modulation of a scene imaged by a computed-tomography imaging spectrometer (CTIS) has been found to be useful as a means of improving the imaging performance of the CTIS. As used here, "spatial modulation" signifies the imposition of additional, artificial structure on a scene from within the CTIS optics. The basic principles of a CTIS were described in "Improvements in Computed- Tomography Imaging Spectrometry" (NPO-20561) NASA Tech Briefs, Vol. 24, No. 12 (December 2000), page 38 and "All-Reflective Computed-Tomography Imaging Spectrometers" (NPO-20836), NASA Tech Briefs, Vol. 26, No. 11 (November 2002), page 7a. To recapitulate: A CTIS offers capabilities for imaging a scene with spatial, spectral, and temporal resolution. The spectral disperser in a CTIS is a two-dimensional diffraction grating. It is positioned between two relay lenses (or on one of two relay mirrors) in a video imaging system. If the disperser were removed, the system would produce ordinary images of the scene in its field of view. In the presence of the grating, the image on the focal plane of the system contains both spectral and spatial information because the multiple diffraction orders of the grating give rise to multiple, spectrally dispersed images of the scene. By use of algorithms adapted from computed tomography, the image on the focal plane can be processed into an image cube a three-dimensional collection of data on the image intensity as a function of the two spatial dimensions (x and y) in the scene and of wavelength (lambda). Thus, both spectrally and spatially resolved information on the scene at a given instant of time can be obtained, without scanning, from a single snapshot; this is what makes the CTIS such a potentially powerful tool for spatially, spectrally, and temporally resolved imaging. A CTIS performs poorly in imaging some types of scenes in particular, scenes that contain little spatial or spectral variation. The computed spectra of such scenes tend to approximate correct values to within acceptably small errors near the edges of the field of view but to be poor approximations away from the edges. The additional structure imposed on a scene according to the present method enables the CTIS algorithms to reconstruct acceptable approximations of the spectral data throughout the scene.

Bearman, Gregory H.↗
NASA NTRS1995

DREAMS and IMAGE: A Model and Computer Implementation for Concurrent, Life-Cycle Design of Complex Systems

Computing architectures are being assembled that extend concurrent engineering practices by providing more efficient execution and collaboration on distributed, heterogeneous computing networks. Built on the successes of initial architectures, requirements for a next-generation design computing infrastructure can be developed. These requirements concentrate on those needed by a designer in decision-making processes from product conception to recycling and can be categorized in two areas: design process and design information management. A designer both designs and executes design processes throughout design time to achieve better product and process capabilities while expanding fewer resources. In order to accomplish this, information, or more appropriately design knowledge, needs to be adequately managed during product and process decomposition as well as recomposition. A foundation has been laid that captures these requirements in a design architecture called DREAMS (Developing Robust Engineering Analysis Models and Specifications). In addition, a computing infrastructure, called IMAGE (Intelligent Multidisciplinary Aircraft Generation Environment), is being developed that satisfies design requirements defined in DREAMS and incorporates enabling computational technologies.

Hale, Mark A.↗