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Brushing Your Spacecrafts Teeth: A Review of Biological Reduction Processes for Planetary Protection Missions

Much like keeping your teeth clean, where you brush away biofilms that your dentist calls plaque, there are various methods to clean spaceflight hardware of biological contamination, known as biological reduction processes. Different approaches clean your hardwares teeth in different ways and with different levels of effectiveness. We know that brushing at home with a simple toothbrush is convenient and has a different level of impact vs. getting your teeth cleaned at the dentist. In the same way, there are some approaches to biological reduction that may require simple tools or more complex implementation approaches (think about sonicating or just soaking your dentures, vs. brushing them). There are also some that are more effective for different degrees of cleanliness and still some that have materials compatibility concerns. In this article, we review known and NASA-certified approaches for biological reduction, pointing out materials compatibility concerns and areas where additional research is needed.

Pugel, D.E. (Betsy)↗

The Quest for Engineering Innovation at NASA's Marshall Space Flight (MSFC)

A recent NASA team, chartered to examine innovation within the Agency, captured the meaning of the word innovation as the "application of creative ideas to improve and generate value for the organization". The former NASA Administrator Charles Bolden shared his own thoughts about innovation in a memo with all employees that stated, "At NASA, we are dedicated to innovation, bold ideas, and excellence." Innovation turns out to be one of the major driving forces behind the work produced at NASA. It seems failure is often what has driven NASA to be more innovative. Fifty years ago, the Apollo 1 tragedy killed three astronauts when fire erupted in their command module. NASA had to bear the responsibility of such loss and at the same time work smarter in order to obtain the dream to reach the moon by the end of the 1960s. Through this circumstance, NASA engineers developed a revolutionary replacement for the combustible nylon astronaut suits so the Apollo program could continue. A material called Beta Cloth was born. This material was used to produce noncombustible space suits for all Apollo astronauts, enabling the United States to ultimately land 12 Americans on the moon. Eventually this material was used as the roof system in the Denver International Airport, showing relevance and applications of NASA innovations to real-world need. Innovative ideas are also driven by the need to accomplish NASA missions and to improve the way we produce our products. MSFC engineers are advancing technologies in additive manufacturing of liquid rocket engines in order to reduce the number of parts, design time, and the cost of the engines. NASA is working with academia to eliminate the need for miles of sensor cables by investigating innovations in wireless sensors. In order to enable future exploration missions to Mars, MSFC engineers are pursuing innovative approaches in diverse areas such as the use of ionic liquids for life support systems and composite cryogenic tanks, very low leakage valves to contain propulsion fluids, and natural, non-toxic inhibitors to eliminate the buildup of biofilms in the water systems planned for future crewed Mars missions. Although results are encouraging, we cannot rest on our past accomplishments. In order to overcome breathtaking technical challenges in space exploration, we must continue to promote a culture supporting growth, breakthroughs and disruptive innovations.

Turner, James E.↗

Marshall Space Flight Center Faculty Fellowship Program

The 2017 Marshall Faculty Fellowship Program involved 21 faculty in the laboratories and departments at Marshall Space Flight Center. These faculty engineers and scientists worked with NASA collaborators on NASA projects, bringing new perspectives and solutions to bear. This Technical Memorandum is a compilation of the research reports of the 2017 Marshall Faculty Fellowship program, along with the Program Announcement (Appendix A) and the Program Description (Appendix B). The research affected the following six areas: (1) Materials (2) Propulsion (3) Instrumentation (4) Spacecraft systems (5) Vehicle systems (6) Space science The materials investigations included composite structures, printing electronic circuits, degradation of materials by energetic particles, friction stir welding, Martian and Lunar regolith for in-situ construction, and polymers for additive manufacturing. Propulsion studies were completed on electric sails and low-power arcjets for use with green propellants. Instrumentation research involved heat pipes, neutrino detectors, and remote sensing. Spacecraft systems research was conducted on wireless technologies, layered pressure vessels, and two-phase flow. Vehicle systems studies were performed on life support-biofilm buildup and landing systems. In the space science area, the excitation of electromagnetic ion-cyclotron waves observed by the Magnetospheric Multiscale Mission provided insight regarding the propagation of these waves. Our goal is to continue the Marshall Faculty Fellowship Program funded by Center internal project offices. Faculty Fellows in this 2017 program represented the following minority-serving institutions: Alabama A&M University and Oglala Lakota College.

Six, N. F.↗

Investigation of Silver Biocide as a Disinfection Technology for Spacecraft-An Early Literature Review

An ideal spacecraft water disinfection system should prevent or control microbial growth, inhibit or prevent biofilm formation, and prevent microbial-induced corrosion. In addition, the selected biocide system should be chemically compatible with materials used in the water storage and distribution system, have minimal maintenance requirements, especially for long-duration missions, and should be safe for crew consumption at levels appropriate for biocidal control. Silver ion is a proven broad spectrum biocide. There has been an increased interest in the biocidal function of silver, both due to its potential to control biocide resistant species and due to advances in silver and nanosilver biocide technologies. NASA is considering silver as the future biocide for exploration over the iodine biocide system. In order to select and design a successful silver biocide delivery system to meet NASA's requirements, it is essential to understand the advantages and disadvantages of moving to a silver disinfection system. To enhance the knowledge base for the application of silver biocides in spacecraft water systems, this paper provides a first compilation of review data related to: (1) Silver as a biocide technology, (2) Options and concepts for silver biocide delivery, and (3) Silver biocide compatibility studies for spacecraft systems.

Li, Wenyan↗

Upgrades to the International Space Station Water Processor Assembly

The International Space Station Water Processor Assembly provides contaminant control and deionization to the Water Recovery System. The Water Processor Assembly presently utilizes sorbent-based Multifiltration Beds and a downstream Catalytic Reactor for these operations. Upgrades and process improvements are desired to improve performance, increase reliability, and decrease consumable resupply. To this end, reverse osmosis membrane separation technologies were evaluated to reduce influent contaminant loads, candidate additives to inhibit wastewater biofilm formation were studied, and life stability testing was completed for a recently developed high-activity catalyst. Evaluation of an adsorption media integration concept was also completed. The performance and applicability of these new technologies within the Water Processor Assembly, as well as their suitability for exploration missions, are discussed herein.

Kayatin, Matthew J.↗

Spaceflight Microbiology

Microbiological Areas of Concern: Astronaut Health; Vehicle integrity; Life support and other systems failure -Biofilm formation/biofouling -Bio-corrosion and biodegradation -Trash and human waste containment -Risk of condensation -Astronaut hygiene areas; Spaceflight foods -Impact of “pick and eat” foods on the environment -Impact of the environment on “pick and eat” foods -Rinsing food with potable water is impractical; Planetary protection -How do we track what we are leaving versus what we are finding in our search for life on other planets?

Ott, C. Mark↗

Silver Foam as Long-Term Passive Biocide for Potable Water Systems

A spacecraft water disinfection system, suitable for extended length space exploration missions, should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically-influenced corrosion. In addition, the system should have minimal maintenance requirements, the effluent should be chemically compatible with all materials in contact with the water, be safe for human consumption, and suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad spectrum biocide. Silver is also the potable water biocide of choice for future exploration missions. Currently, the proposed method for implementing silver biocide in spacecraft systems relies on silver electrode technology to produce a controlled amount of silver ions. Unfortunately, electrolytic-based silver dosing presents multiple inherent challenges that affect performance and increase maintenance requirements over time. To decrease the risk of failure, an alternative silver biocide delivery method is needed. Control-release technology is an attractive option for developing a passive high-reliability silver dosing device. The concept of a nanoparticle/polyurethane (PU) composite foam for the controlled release of silver was prompted by the controlled release technology developed by NASA for the delivery of corrosion inhibitors and indicators. This paper presents the technical background and results from the synthesis and properties testing of the silver nanoparticles (AgNPs)/PU composite foam that is being developed for use in spacecraft potable water systems.

Irwin, Tesia D.↗

How Does Water Delivery System Design Impact the Microbial Load of Salad Crops?

In a microgravity setting, such as the environment aboard the International Space Station (ISS), an ideal plant water delivery system is one that can grow edible crops with minimal resource consumption and minimal risk to crew members. There are also concerns associated with the ability to control fluid escape and biofilm formation resulting in potential dangers to systems, crops, or crewmembers. To identify an appropriate system, candidate systems were assembled and operated under simulated ISS environmental conditions (T,CO2,and RH) with red romaine lettuce (Lactuca sativa cultivar 'Outredgeous') as a model crop. Fluid reservoirs and randomly selected planting sites were sampled every seven days until maturity at which point edible plant biomass and root samples were also taken. Heterotrophic bacteria and fungi growth patterns throughout each planting cycle were determined by plate counts on appropriate agar media. The candidate systems were compared to a classic hydroponics system as a control and harvested crops were compared to controls as well as Veggie-grown and market produce. Plants harvested from candidate systems yielded lower average heterotrophic bacteria and fungi per gram of plant mass levels when compared to market and Veggie samples as well as those from the control system. Additional studies to evaluate the system sanitation regimen as well as testing additional crops should be considered to aid in the selection of an ideal system.

Curry, Aaron B.↗

Results of the Micro-12 Flight Experiment: Effects of Microgravity on Shewanella oneidensis MR-1

The Micro-12 flight experiment was launched on SpaceX-15 and completed during berthing on the International Space Station. The goal of this experiment was to understand the effects of spaceflight and microgravity on the physiology of the model exoelectrogen Shewanella oneidensis MR-1. BioServe Fluid Processing Apparatus (FPA) and Group Activation Pack (GAP) hardware systems were used for both flight and ground control tests. Under spaceflight conditions, extracellular electron transfer (EET) rates were found to be significantly increased on insoluble substrates, while biofilm development appeared to be unchanged under the conditions tested; these processes are critical for microbial-assisted bioelectrochemical systems. Additionally, RNAseq analysis, proteomic profiling, and competitive mutant fitness profiling were performed to gain further understanding of microbial physiology under EET-respiring conditions during spaceflight. Overall, the results of the Micro-12 project support the idea that Shewanella oneidensis MR-1, in particular, and exoelectrogens in general could be useful chassis organisms for synthetic biology applications using microbial bioelectrochemical systems. These findings will assist bioengineering and synthetic biology development efforts harnessing the unique capabilities of exoelectrogens for life support and in situ resource utilization.

Dougherty, Michael↗

Silver Foam A Long-Term Passive Biocide for Potable Water Systems: 2020 Update

A spacecraft water disinfection system suitable for extended length space exploration missions should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically-influenced corrosion. In addition, the system should have minimal maintenance requirements, the effluent should be chemically compatible with all materials in contact with the water, be safe for human consumption, and suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad spectrum biocide and the chosen potable water biocide for future exploration missions. The leading technology for actively dosing silver in future water systems is based on electrolytic production. Several challenges remain with this approach which have prompted additional investigations into alternative dosing techniques. Control-release technology is an attractive option for developing a high-reliability passive silver dosing device. This paper describes the development of a nanoparticle (NP)/polyurethane (PU) composite foam for the controlled release of silver ions, and is intended to build upon the 2019 International Conference on Environmental Systems (ICES) paper of the same name. In this paper, the technical background and results from the updated silver chloride (AgCl) NP/PU composite foam synthesis and property testing is provided. The ultimate goal of the project is to develop a stable and reliable passive dosing silver ion release device for use in future spacecraft potable water systems.

Irwin, Tesia D.↗

Silver Foam: A Novel Approach for Long-Term Passive Dosing of Biocide in Spacecraft Potable Water Systems

A spacecraft water disinfection system suitable for extended length space exploration should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically influenced corrosion. In addition, the system should have minimal maintenance requirements,should be chemically compatible with all materials in contact with the water, be safe for human consumption, and suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad-spectrum biocide under consideration as a potable water biocide for future exploration missions. The leading technology for actively dosing silver in future water systems is based on electrolytic production. Several challenges remain with this approach that have prompted additional investigations into alternative dosing techniques. Control-release technology is an attractive option for developing a high-reliability passive silver dosing device. This paper describes the development of a silver chloride (AgCl) nanoparticle (NP)/polyurethane (PU) composite foam for the controlled release of silver ions, and is intended to build upon the 2019 International Conference on Environmental Systems (ICES) paper number 272.This paper provides the technical background and results from the updated AgClNP/PU composite foam (AgFoam) synthesis and property testing. The ultimate goal of the project is to develop a stable and reliable passive dosing silver ion release device for use in future spacecraft potable water systems.

Tesia Dawn Irwin↗

Algorithmic Detection of Elemental Biosignatures

Machine learning models that classify a sample as indicative or non-indicative of life could play an important role in life-detection missions. Their predictions result from agnostic algorithms and thereby add redundancy to judgements resulting from human expertise. Additionally, their important features can reveal the most informative measurements within the operational constraints of a life-detection mission. The Ladder of Life Detection (Neveu 2018) identifies the need for an understanding of how combinations of multiple biosignatures affect overall confidence. The present work provides a starting point to answer this need, and future work will expand the data types to obtain even more predictive combinations of features. Elemental abundance was chosen as a starting set of features due to its availability in diverse sample types, which are needed to train a generalizable model. A standardized dataset was collected, including 35 non-indicative, e.g., lunar rock, basalt; 19 indicative mixed, e.g., seawater, agricultural soil; 46 indicative non-alive, e.g., coal, chalk; and 10 indicative alive, e.g., biofilm, bacteria. This dataset could be valuable for complementary biosignature research. The samples were standardized to the same limit of detection of a simulated mission scenario. Four classification models were used: k-nearest neighbors (KNN), logistic regression (LR), linear support vector machines (SVM), and Gaussian naïve Bayes (GNB). To obtain feature importances, KNN was run on three principal components of the training data and LR and SVM were run with L1 and L2 regularization. The performances and feature importances of the six model variants on 40:60 train to validation ratios were assessed with Monte Carlo simulations. ROC AUC and mean accuracy scores ranged between 82% - 94%, with sensitivity greater than specificity. For indicative of life predictors, all models had C and Ca as strong and Cl as medium; a majority of models had N, K, and P as medium. For non-indicative of life predictors, all models had Si as strong, and a majority of models had Mg, Al, and Ti as medium. Varied elements were Fe (slightly non-indicative), H (slightly indicative), O (widely varied), Na, Mn, and S. These results serve as a proof of concept and suggest important elemental signals beyond merely the CHNOPS of Earth-based life.

Algorithmic↗

Development Requirements for the Exploration PLSS (xPLSS) Thermal Loop Filter

The fluid thermal control loop and auxiliary thermal control loop contain multiple filters including the Thermal Filters (F-448/F-548), which are used to scrub precipitates and biofilms from the cooling loops in order to protect components that may be sensitive to such contamination. The F-448/F-548 filters are comprised of multiple machined piece parts to create the filter housing as well as the removable pressure cap assembly to allow for the thermal filter cartridge replacement.

Kristina Noelle Todd↗

Common Scientific and Technological Interests Between Astrobiology and Space Biology

The disciplines of astrobiology (AB) and space biology (SB) clearly have common interests, however they have not been pursued jointly. SB and AB are inextricably linked, both intellectually and technologically. They can now be effectively linked operationally. Cross-cutting joint collaborations will enhance innovation and increase cost effectiveness. Session topics include joint science questions, technologies, instrumentation, and missions. Examples include life detection, overlapping planetary protection concerns, biofilms, radiation, hyper- and hypogravity, applications of artificial intelligence and machine learning, interoperable databases, facilities (i.e., spacecraft, lunar surface efforts, simulation chambers, analog sites, etc.), training opportunities, and other topics relevant to AB and SB joint ventures. We welcome contributions on this very broad topical area to facilitate cross-fertilization of these disciplines that are of great importance to NASA.

astrobiology↗

Spaceflight-Induced Changes in Microbial Virulence and the Impact to the Host Immune Response

Over the past 50 years, microorganisms have displayed unexpected responses relevant to infectious disease when grown in spaceflight and spaceflight analogues, including changes in final cell concentration, biofilm production, stress resistance, antibiotic sensitivity, gene expression, and virulence. •Seminal studies demonstrated that the foodborne pathogen, Salmonella enterica serovar Typhimurium, increased its virulence and pathogenesis-related characteristics in response to both spaceflight and spaceflight analogue culture. Since those experiments, alterations in the pathogenesis-related characteristics of other pathogens have been documented in response to growth in these environments4,5,6, and a recent study has demonstrated an increase in virulence of Serratia marcescens cultured during spaceflight7. However, our overall knowledge of which microorganisms will alter their virulence in response to spaceflight and spaceflight analogue culture remains very limited

C. M. Ott↗

Worswick Hot Springs: A Radioactive Hydrothermal Field Site.

Introduction: We report on a systematic characterization of the environmental conditions at Worswick Hot Springs, a hydrothermal system in Idaho, USA. Because localized “hot spots” of elevated radiation and biofilms are easily accessible, various biological studies of radiation resistance and biosignature formation are possible, making this fieldsite relevant for analog field studies that consider microbiology, geo-chemistry, and ionizing radiation. In addition to Worswick being a natural radiation biology laboratory that may also be relevant for space biology applications, we assert that these unusual environmental conditions may inform us about locations on Mars that are also enriched in radioactive elements and their poten-tial for hosting biosignatures. Methods: We carried out repeated temperature and radiation measurements at the same hot spring locations to observe the system over time (Figure 2). Radiation: A Bicron Micro Analyst micro-r-meter (Bicron NE, Saint-Gobain Industrial Ceramics, Inc.) capable of sensing x-rays and gamma-rays (0-5000 µR/hr), was used to gather radiation data at twenty-four locations around and above the region of the two main stream channels. Water Temperature: A digital hand-held infrared thermometer (Oakton WD-39642-00 Mini-Temp Tester) was used for all measurements. ICP-MS aqueous geochemistry: Water samples were analyzed at the ISU Center for Archaeology, Materials and Applied Spectroscopy (CAMAS). Results: We have discovered localized areas of elevated radiation that are approximately 4 to 5 times greater than background radiation, and we have ob-served that both radioactivity and temperature of the spring waters vary over time. ICP-MS reveal the presence of thorium and uranium, which are correlated with elevated radioactivity. Several point sources of elevated radioactivity have been identified in both Stream A and Stream B.

Analog↗

Silver Foam: A Novel Approach for Long-Term Passive Dosing of Biocide in Spacecraft Potable Water Systems

A spacecraft water disinfection system, suitable for extended length space exploration, should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically influenced corrosion. In addition, the system should have minimal maintenance requirements, be chemically compatible with all materials in contact with the water, be safe for human consumption, and be suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad-spectrum potable water biocide under investigation for future exploration missions. The competing technology for dosing silver ions in future water systems is based on actively dosing the ions via electrolytic production. Several challenges with this approach have prompted additional investigations into alternative dosing techniques. Control-release technology is an attractive option for developing a high-reliability passive silver dosing device. This paper describes the development of a nanoparticle/polyurethane (NP/PU) composite foam for the controlled release of silver ions, and is intended to build upon the 2020 International Conference onEnvironmental Systems (ICES) paper number 128. This paper provides the technical background and performance results from the updated silver chloride (AgCl) NP/PU. The ultimate goal of the project is to develop a stable and reliable passive dosing silver ion release device for use in future spacecraft potable water systems.

Tesia D. Irwin↗

Water Shelf Life for Long-Duration Rodent Research Missions

The National Aeronautics and Space Administration Rodent Research Project habitat is a highly-specialized rodent husbandry caging system that enables mice to be flown to the International Space Station for a variety of bioscience studies in the unique microgravity and radiation environment. The habitat accommodates an internal water supply box containing four water access points and two one liter flexible plastic sterile blood bags for storage. Two access points are provided on each side of the habitat, which accommodates five animals per side. Due to the nature of operations on the Space Station – including limited fluid handling – water cannot be changed out weekly as in ground-based vivariums. Water is added to the system a maximum of eighteen months prior to being provided to rodents. The dependable provision of high-quality drinking water is essential for both the welfare of rodents and the scientific integrity of the studies in which they are involved. The robust, multi-stage water preparation process involves the autoclave sterilization of deionized water, the addition of iodine to minimize biofilms, and filtration through a sterile filter to ensure removal of bacteria and other contaminants. The water is then loaded into the sterile blood bags as part of the automated delivery system. A water storage test determined that the shelf life of this water in the habitat could be increased up to four years from a previous eighteen months. This facilitates the ability to pre-position habitats on Station as well as demonstrates that the habitat’s water system can be utilized on future long-duration and deep space missions involving rodents.

Tyler Marsh↗