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Ultrahigh-resolution mass spectrometry data associated with the manuscript “A functional microbiome catalog crowdsourced from North American rivers"

This data package is associated with the publication “A functional microbiome catalog crowdsourced from North American rivers” submitted to Nature (Borton et al., 2024); (https://www.biorxiv.org/content/10.1101/2023.07.22.550117v1). Predicting elemental cycles and maintaining water quality under increasing anthropogenic influence requires understanding the spatial drivers of river microbiomes. However, the unifying microbial determinants governing river biogeochemistry are hindered by a lack of genome-resolved functional insights and sampling across multiple rivers. Here we employed a community science effort to accelerate the sampling of river microbiomes to create the Genome Resolved Open Watersheds database (GROWdb). GROWdb is a publicly available resource that paves the way for watershed predictive modeling and microbiome-based management practices. This resource profiled the identity, distribution, function, and expression of thousands of microbial genomes across rivers covering 90% of United States watersheds. We identified the most cosmopolitan microbiome members, while also revealing local drivers of strain endemism across ecological dimensions. We provide the first evidence that microbial functional trait expression followed the tenets of the River Continuum Concept, suggesting the structure and function of river microbiomes is predictable. The Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) data were one of many different data types used in establishing the ecological dimensions along which different microbes were detected .This data package only contains the processed FTICR-MS data associated with this manuscript; all other data is accessible via Zenodo (https://zenodo.org/records/8173287), GitHub (https://github.com/jmikayla1991/Genome-Resolved-Open-Watersheds-database-GROWdb), KBase (https://doi.org/10.25982/109073.30/1895615), and NCBI via Bioproject PRJNA946291.This dataset consists of (1) a file-level metadata (flmd) file; (2) a data dictionary (dd) file; (3) a readme; (4) three Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) processed data files (a ‘data’ file containing peak-by-sample observations, a ‘mol’ file containing peak metadata, and a transformation profile containing transformation-by-sample observations). All files are .csv or .pdf.

54 ENVIRONMENTAL SCIENCES↗

Determining Unabated Airborne Radionuclide Emissions Monitoring Requirements Using Inventory-Based Methods

Compliance with the airborne radionuclide emission monitoring requirements in the National Emission Standards for Hazardous Air Pollutants (NESHAP; Title 40 of the U.S. Code of Federal Regulations Part 61, Subpart H) and State requirements in Washington Administrative Code 246–247: Radiation Protection – Air Emissions and 173-480: Ambient Air Quality Standards and Emission Limits for Radionuclides were evaluated for Pacific Northwest National Laboratory (PNNL) operations. Additional guidance may be found in the U.S. Department of Energy Handbook, Environmental Radiological Effluent Monitoring and Environmental Surveillance. To meet regulatory requirements, reviews of planned or proposed projects and activities provide the basis for implementing necessary monitoring adjustments or for implementing changes to projects and activities in a timely manner. Potential unabated off-site doses were evaluated for emission locations managed by PNNL and licensed to the Department of Energy. These locations were at facilities in Richland, Washington (i.e., the Hanford Site and PNNL–Richland campus) and in Sequim, Washington, (PNNL–Sequim campus). This report describes the inventory-based methods and provides the results for the NESHAP assessment performed in January 2024 for calendar year ending 2023.

40 CFR 61 Subpart H↗

Towards a standard approach to the analysis of carbon products generated from Natural Gas pyrolysis

Conference presentation for the work entitled “A standard approach to the analysis of carbon products generated from Natural Gas pyrolysis”. Pyrolysis of natural gas (NGP) has been proposed as a near term solution for the production of direct CO2 free (turquoise) hydrogen, to help fuel a more sustainable energy future. NGP can serve as a bridging technology while other (green) hydrogen production technologies mature. In order for NGP to be successful, the management of the generated carbon products needs to be carefully considered. Necessary markets need to be considered to incorporate the (turquoise) carbon products that are generated. A large hurdle in addressing the channeling of carbon products into desired markets is determining exactly what is manufactured from the NGP process. In this work an analysis method is presented that combines a suite of techniques for the assessment of the carbon product from Natural Gas Pyrolysis for appropriate identification and quality control.

Riley, Jarrett↗

The Effects of Beta Irradiation on the Performance of ATR Fiber Probes

The use of real-time inline monitoring to track the chemical composition of slurries is recognized as a promising method to reduce the processing time of Hanford tank waste. The conversion of waste into glass suitable for long-term storage requires adherence to waste acceptance criteria and process control limits that respectively ensure safe operation of the vitrification facility and suitable glass quality. Real-time measurements of blended waste materials can ensure that the various criteria are met or can be addressed in a timely manner.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Scientific and technical photography at NASA Langley Research Center

As part of my assignment connected with the Scientific and Technical Photography & Lab (STPL) at the NASA Langley Research Center I conducted a series of interviews and observed the day to day operations of the STPL with the ultimate objective of becoming exposed first hand to a scientific and technical photo/imaging department for which my school prepares its graduates. I was also asked to share my observations with the staff in order that these comments and observations might assist the STPL to better serve its customers. Meetings with several individuals responsible for various wind tunnels and with a group that provides photo-optical instrumentation services at the Center gave me an overview of the services provided by the Lab and possible areas for development. In summary form these are some of the observations that resulted from the interviews and daily contact with the STPL facility. (1) The STPL is perceived as a valuable and almost indispensable service group within the organization. This comment was invariably made by everyone. Everyone also seemed to support the idea that the STPL continue to provide its current level of service and quality. (2) The STPL generally is not perceived to be a highly technically oriented group but rather as a provider of high quality photographic illustration and documentation services. In spite of the importance and high marks assigned to the STPL there are several observations that merit consideration and evaluation for possible inclusion into the STPL's scope of expertise and future operating practices. (1) While the care and concern for artistic rendition of subjects is seen as laudable and sometimes valuable, the time that this often requires is seen as interfering with keeping the tunnels operating at maximum productivity. Tunnel managers would like to shorten down-time due to photography, have services available during evening hours and on short notice. It may be of interest to the STPL that tunnel managers are incorporating ever greater imaging capabilities in their facilities. To some extent this could mean a reduced demand for traditional photographic services. (2) The photographic archive is seen as a Center resource. Archiving of images, as well as data, is a matter of concern to the investigators. The early holdings of the Photographic Archives are quickly deteriorating. The relative inaccessibility of the material held in the archives is problematic. (3) In certain cases delivery or preparation of digital image files instead of, or along with, hardcopy is already being perceived by the STPL's customers as desirable. The STPL should make this option available, and the fact that it has, or will have this capability widely known. (4) The STPL needs to continue to provide expert advice and technical imaging support in terms of application information to users of traditional photographic and new electronic imaging systems. Cooperative demo projects might be undertaken to maintain or improve the capabilities of the Lab. (5) STPL personnel do not yet have significant electronic imaging or electronic communication skills and improvements in this is an area could potentially have a positive impact on the Center. (6) High speed photographic or imaging services are often mentioned by the STPL as being of primary importance to their mission but the lab supports very few projects calling for high speed imaging services. Much high speed equipment is in poor state of repair. It is interesting to note that when the operation of lasers, digital imaging or quantitative techniques are requested these are directed to another NASA department. Could joint activities be initiated to solve problems? (7). The STPL could acquire more technical assignments if examples of the areas where they posses expertise would be circulated around the center. The fact that the STPL owns high speed video capability could be 'advertised' among its customer base if there truly was an interest in building up a customer base in this area. The STPL could participate in events like TOPS as an exhibitor, as well as a documenter, of the event.

Davidhazy, Andrew↗

Land Use Planning

Computer technology, aerial photography and space imagery are being combined in a NASA community services program designed to help solve land use and natural resource planning problems. As urban areas grow, so grows the need for comprehensive, up-to-date information on which to base intelligent decisions regarding land use. State and local planners need information such as the nature of urban change, where the changes are occurring, how they affect public safety, transportation, the economy, tax assessment, sewer systems, water quality, flood hazard, noise impact and a great variety of other considerations. Most importantly they need continually updated maps. Preparing timely maps, gathering the essential data and maintaining it in orderly fashion are becoming matters of increasing difficulty. The NASA project, which has nationwide potential for improving efficiency in the planning process, is a pilot program focused on Tacoma, Washington and surrounding Pierce County. Its key element, developed by Jet Propulsion Laboratory (JPL), is a computerized Land Use Management Information System (LUMIS).

Source record↗

The Application of NASA Remote Sensing Technology to Human Health

With the help of satellites, the Earth's environment can be monitored from a distance. Earth observing satellites and sensors collect data and survey patterns that supply important information about the environment relating to its affect on human health. Combined with ground data, such patterns and remote sensing data can be essential to public health applications. Remote sensing technology is providing information that can help predict factors that affect human health, such as disease, drought, famine, and floods. A number of public health concerns that affect Earth's human population are part of the current National Aeronautics and Space Administration (NASA) Earth Science Applications Plan to provide remotely gathered data to public health decision-makers to aid in forming and implementing policy to protect human health and preserve well-being. These areas of concern are: air quality; water quality; weather and climate change; infectious, zoonotic, and vector-borne disease; sunshine; food resource security; and health risks associated with the built environment. Collaborations within the Earth Science Applications Plan join local, state, national, or global organizations and agencies as partners. These partnerships engage in projects that strive to understand the connection between the environment and health. The important outcome is to put this understanding to use through enhancement of decision support tools that aid policy and management decisions on environmental health risks. Future plans will further employ developed models in formats that are compatible and accessible to all public health organizations.

Watts, C. T.↗

Monitoring Phenology as Indicator for Timing of Nutrient Inputs in Northern Gulf Watersheds

Nutrient over-enrichment defined by the U.S. Environmental Protection Agency as the anthropogenic addition of nutrients, in addition to any natural processes, causing adverse effects or impairments to the beneficial uses of a water body has been identified as one of the most significant environmental problems facing sensitive estuaries and coastal waters. Understanding the timing of nutrient inputs into those waters through remote sensing observables helps define monitoring and mitigation strategies. Remotely sensed data products can trace both forcings and effects of the nutrient system from landscape to estuary. This project is focused on extracting nutrient information from the landscape. The timing of nutrients entering coastal waters from the land boundary is greatly influenced by hydrologic processes, but can also be affected by the timing of nutrient additions across the landscape through natural or anthropogenic means. Non-point source nutrient additions to watersheds are often associated with specific seasonal cycles, such as decomposition of organic materials in fall and winter or addition of fertilizers to crop lands in the spring. These seasonal cycles or phenology may in turn be observed through the use of satellite sensors. Characterization of the phenology of various land cover types may be of particular interest in Gulf of Mexico estuarine systems with relatively short pathways between intensively managed systems and the land/estuarine boundary. The objective of this study is to demonstrate the capability of monitoring phenology of specific classes of land, such as agriculture and managed timberlands, at a refined watershed level. The extraction of phenological information from the Moderate Resolution Imaging Spectroradiometer (MODIS) data record is accomplished using analytical tools developed for NASA at Stennis Space Center: the Time Series Product Tool and the Phenological Parameters Estimation Tool. MODIS reflectance data (product MOD09) were used to compute the Normalized Difference Vegetation Index, which is sensitive to changes in vegetation canopies. The project team is working directly with the Mississippi Department of Environmental Quality to understand end-user requirements for this type of information product. Initial focus areas are identification of time frames for pre-plant fertilizer applications (prior to start of season), side-dress fertilizer applications (during rapid green-up), and periods of plant decomposition (during and after senescence). Prototypical maps of phenological stages related to these time frames have been generated for watersheds in the northern Gulf of Mexico. Where feasible, these maps have been compared to existing in situ nutrient monitoring data, but the in situ data is temporally sparse (monthly frequency or less), which makes interpretation challenging. Future work will include integrating effects of rainfall and seeking couplings with estuarine remote sensing.

Ross, Kenton W.↗

Orion Capsule Handling Qualities for Atmospheric Entry

Two piloted simulations were conducted at NASA's Johnson Space Center using the Cooper-Harper scale to study the handling qualities of the Orion Command Module capsule during atmospheric entry flight. The simulations were conducted using high fidelity 6-DOF simulators for Lunar Return Skip Entry and International Space Station Return Direct Entry flight using bank angle steering commands generated by either the Primary (PredGuid) or Backup (PLM) guidance algorithms. For both evaluations, manual control of bank angle began after descending through Entry Interface into the atmosphere until drogue chutes deployment. Pilots were able to use defined bank management and reversal criteria to accurately track the bank angle commands, and stay within flight performance metrics of landing accuracy, g-loads, and propellant consumption, suggesting that the pilotability of Orion under manual control is both achievable and provides adequate trajectory performance with acceptable levels of pilot effort. Another significant result of these analyses is the applicability of flying a complex entry task under high speed entry flight conditions relevant to the next generation Multi Purpose Crew Vehicle return from Mars and Near Earth Objects.

Tigges, Michael A.↗

Performance of Water Recirculation Loop Maintentance Components for the Advanced Spacesuit Water Membrane Evaporator

Water loop maintenance components to maintain the water quality of the Advanced Spacesuit Water Membrane Evaporation (SWME) water recirculation loop have undergone a comparative performance evaluation with a second SWME water recirculation loop with no water quality maintenance. Results show the benefits of periodic water maintenance. The SWME is a heat rejection device under development at the NASA Johnson Space Center to perform thermal control for advanced spacesuits. One advantage to this technology is the potential for a significantly greater degree of tolerance to contamination when compared to the existing Sublimator technology. The driver for the evaluation of water recirculation maintenance components was to further enhance this advantage through the leveraging of fluid loop management lessonslearned from the International Space Station (ISS). A bed design that was developed for a UTAS military application, and considered for a potential ISS application with the Urine Processor Assembly, provided a low pressure drop means for water maintenance in a recirculation loop. The bed design is coupled with high capacity ion exchange resins, organic adsorbents, and a cyclic methodology developed for the Extravehicular Mobility Unit (EMU) Transport Water loop. The maintenance cycle included the use of a biocide delivery component developed for ISS to introduce a biocide in a microgravity-compatible manner for the Internal Active Thermal Control System (IATCS). The leveraging of these water maintenance technologies to the SWME recirculation loop is a unique demonstration of applying the valuable lessons learned on the ISS to the next generation of manned spaceflight Environmental Control and Life Support System (ECLSS) hardware.

Rector, Tony↗

Performance of Water Recirculation Loop Maintenance Components for the Advanced Spacesuit Water Membrane Evaporator

Water loop maintenance components to maintain the water quality of the Advanced Spacesuit Water Membrane Evaporation (SWME) water recirculation loop have undergone a comparative performance evaluation with a second SWME water recirculation loop with no water quality maintenance. Results show the benefits of periodic water maintenance. The SWME is a heat rejection device under development at the NASA Johnson Space Center to perform thermal control for advanced spacesuits. One advantage to this technology is the potential for a significantly greater degree of tolerance to contamination when compared to the existing Sublimator technology. The driver for the evaluation of water recirculation maintenance components was to further enhance this advantage through the leveraging of fluid loop management lessons learned from the International Space Station (ISS). A bed design that was developed for a UTAS military application, and considered for a potential ISS application with the Urine Processor Assembly, provided a low pressure drop means for water maintenance in a recirculation loop. The bed design is coupled with high capacity ion exchange resins, organic adsorbents, and a cyclic methodology developed for the Extravehicular Mobility Unit (EMU) Transport Water loop. The maintenance cycle included the use of a biocide delivery component developed for ISS to introduce a biocide in a microgravity compatible manner for the Internal Active Thermal Control System (IATCS). The leveraging of these water maintenance technologies to the SWME recirculation loop is a unique demonstration of applying the valuable lessons learned on the ISS to the next generation of manned spaceflight Environmental Control and Life Support System (ECLSS) hardware.

Rector, Tony↗

Space Robot Operating System (Space ROS)

Space Robot Operating System (Space ROS) is an open-source software framework for flight-quality robotic and autonomous space systems. It combines the benefits of open-source software and flight software best-practices into a single framework. Space ROS is predicated on ROS2 and is therefore reusable, modular and adoptable. As an open-source framework, it is accessible to the global space community. Space ROS is intended to be compliant with NASA flight software engineering practices in order to facilitate meeting mission and safety standards. Space ROS has fault management and real-time capabilities at its core.

Will Chambers↗

National Energy Water Treatment & Speciation (NEWTS): A Water & Critical Mineral Database and Dashboard

The scarcity of water resources, the need for beneficial water reuse, and the challenges of wastewater treatment are becoming increasingly pressing in economic, social, and environmental domains. Addressing these concerns requires effective treatment strategies to manage wastewater streams and tackle environmental and economic issues. Furthermore, the recovery of critical minerals from the waste streams associated with energy production holds the promise of offsetting treatment costs and securing local sources of valuable minerals. However, relevant data on these waste streams are dispersed and challenging to locate. The process of ingesting such data into modeling software often involves multiple steps, requiring data restructuring to meet software-input requirements. The non-standardized reporting of water data makes data aggregation and reformatting a time-consuming process. Additionally, essential attributes necessary for modeling water treatment and mineral scale formation are frequently missing. Moreover, data gaps vary depending on the region of interest. Consequently, there is a pressing need for high-quality energy-water composition data that can be easily imported into water chemistry modeling software. To address this need, the National Energy Technology Laboratory has created the National Energy Water Treatment and Speciation (NEWTS) Database and Dashboard—a free online tool catering to community leaders and water researchers. NEWTS facilitates a comprehensive understanding of the composition of energy-related wastewater streams in the United States. The datasets provide detailed concentrations and speciation of major and minor aqueous compounds in energy-related wastewater streams, including power plant leachate, acid mine drainage, brackish water, and oil and gas produced water across the United States. Many of the aqueous species are critical minerals (Li, REEs) in high demand to modernize the world’s energy infrastructure. Many of the datasets also contain volumetric flow-rates needed to model the treatment and reuse scenarios in advanced aqueous chemistry software programs. The NEWTS Database and Dashboard offer public access to hitherto challenging-to-access datasets, presented in a standardized format that is tailored for easy input into aqueous chemistry modeling software. By performing the work needed to transform dispersed, disparate data sources into unified, model-ready datasets, NEWTS serves as an essential resource in advancing water treatment research and sustainable water resource management.

produced water management↗

How deep to dig: effects of web-scraping search depth on hyperlink network analysis of environmental stewardship organizations

Abstract Social network analysis (SNA) tools and concepts are essential for addressing many environmental management and sustainability issues. One method to gather SNA data is to scrape them from environmental organizations’ websites. Web-based research can provide important opportunities to understand environmental governance and policy networks while potentially reducing costs and time when compared to traditional survey and interview methods. A key parameter is ‘search depth,’ i.e., how many connected pages within a website to search for information. Existing research uses a variety of depths and no best practices exist, undermining research quality and case study comparability. We therefore analyze how search depth affects SNA data collection among environmental organizations, if results vary when organizations have different objectives, and how search depth affects social network structure. We find that scraping to a depth of three captures the majority of relevant network data regardless of an organization’s focus. Stakeholder identification (i.e., who is in the network) may require less scraping, but this might under-represent network structure (i.e., who is connected). We also discuss how scraping web-pages of local programs of larger organizations may lead to uncertain results and how our work can combine with mixed methods approaches.

Sayles, Jesse S. (ORCID:0000000218378920)↗

Evidence for the existence and ecological relevance of fast-cycling mineral-associated organic matter

Longstanding theories and models classify mineral-associated organic matter as the large ( ~ 60%) but slow-cycling and persistent portion of soil organic matter. Strong physico-chemical interactions and diffusion limitations restrict the turnover of mineral-associated organic matter, allowing carbon and nitrogen bound therein to persist in soil for as long as centuries to millennia. However, mineral-associated organic matter is a chemically and functionally diverse pool with a substantial portion cycling at relatively fast (i.e., minutes to years) timescales. Despite a growing body of evidence for the heterogenous and multi-pool nature of mineral-associated organic matter, we lack consensus on how to conceptualize and directly quantify fast-cycling mineral-associated organic matter and its ecological significance. We demonstrate that the dynamic qualities of fast-cycling mineral-associated organic matter vary based on 1) the chemistry of the mineral particles and organic matter, 2) the complex set of interactions between organic matter and the mineral matrix, and 3) the presence and strength of destabilizing forces that lead to decomposition or loss of mineral-associated organic matter (i.e., plant-microbe interactions, agricultural intensification, and climate change). Finally, we discuss potential implications and research opportunities for how we measure, manage, and model the dynamic subfraction of this otherwise persistent pool of soil organic matter.

Biological and medical sciences↗

Pacific Northwest National Laboratory Annual Site Environmental Report for Calendar Year 2024

The report provides a synopsis of ongoing environmental management performance and compliance activities for operations that occur at the PNNL-Richland campus in Richland, Washington, and at the PNNL-Sequim campus near Sequim, Washington. It describes the location of and background for each facility; addresses compliance with applicable DOE, federal, state, and local regulations, and site-specific permits; documents environmental monitoring efforts and their status; presents potential radiation doses to staff and the public in the surrounding areas; and describes DOE-required data quality assurance methods used for data verification.

54 ENVIRONMENTAL SCIENCES↗

National Renewable Energy Laboratory, South Table Mountain Campus, Golden, Colorado

The National Renewable Energy Laboratory (NREL) South Table Mountain campus, located in Golden, Colorado, has multiyear datasets from buildings with approximately 1,100,000 sq ft of floor area. These 16 buildings include a large office, nine laboratories, and other education or public assembly facilities such as an education center, a warehouse, quick service restaurants, two site entrance security buildings, and a parking garage—all constructed and improved from 1982 to 2014. These datasets are also available from the NREL data storage and management platform SkySparks. The datasets contain electricity meter data (poser, voltage, and current) with 1-minute resolution and building automation system (BAS) data with resolutions of 5 or 15 minutes. Some subsystems (e.g., lighting) or equipment electricity data have the same intervals as above. Some mechanical equipment state or condition data, such as flow and temperature, are captured with 5- to 15-minute intervals. The types of measurement points (i.e., electrical meter power and zone temperature) and associated data quality across the campus are typical, which helps reduce the unreliability of data quality.

STM, Systems and equipment operational, Energy use↗

Teachers’ Beliefs and Their Influence on Math Instructions for Gifted English Learners

The dynamic interplay between teachers’ beliefs and practices significantly impact the quality of instruction and the trajectory of talent development in young students. This case study explores the beliefs and practices of two elementary teachers instructing gifted ELs in mathematics. The constant comparison method was used to analyze data collected from classroom observations, semi-structured interviews, and field notes. Three factors were found to affect the (in)consistency between teachers’ expressed beliefs and observed practices: compatibility among core and peripheral beliefs, knowledge about evidence-based practices, and classroom management skills. Students exhibit higher levels of participation, communication, and engagement in critical thinking skills when their teacher embraces constructive perspectives in teaching mathematics, demonstrates pedagogical expertise, and employs a proactive classroom management approach. Conversely, students encounter restricted opportunities to independently construct their own understanding of mathematics when their teacher holds maladaptive beliefs about teaching mathematics, has limited knowledge of evidence-based practices, and has an authoritarian classroom management style. These findings underscore the need for a new approach to professional development (PD) that encourages teachers to critically examine the connection between their beliefs and instructional practices and their impact on the student’s mathematical talent development.

Yang, Jenny (ORCID:0000000296905466)↗

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