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Temporal Dependence of Chromosomal Aberration on Radiation Quality and Cellular Genetic Background

Radiation induced cancer risks are driven by genetic instability. It is not well understood how different radiation sources induce genetic instability in cells with different genetic background. Here we report our studies on genetic instability, particularly chromosome instability using fluorescence in situ hybridization (FISH), in human primary lymphocytes, normal human fibroblasts, and transformed human mammary epithelial cells in a temporal manner after exposure to high energy protons and Fe ions. The chromosome spread was prepared 48 hours, 1 week, 2 week, and 1 month after radiation exposure. Chromosome aberrations were analyzed with whole chromosome specific probes (chr. 3 and chr. 6). After exposure to protons and Fe ions of similar cumulative energy (??), Fe ions induced more chromosomal aberrations at early time point (48 hours) in all three types of cells. Over time (after 1 month), more chromosome aberrations were observed in cells exposed to Fe ions than in the same type of cells exposed to protons. While the mammary epithelial cells have higher intrinsic genetic instability and higher rate of initial chromosome aberrations than the fibroblasts, the fibroblasts retained more chromosomal aberration after long term cell culture (1 month) in comparison to their initial frequency of chromosome aberration. In lymphocytes, the chromosome aberration frequency at 1 month after exposure to Fe ions was close to unexposed background, and the chromosome aberration frequency at 1 month after exposure to proton was much higher. In addition to human cells, mouse bone marrow cells isolated from strains CBA/CaH and C57BL/6 were irradiated with proton or Fe ions and were analyzed for chromosome aberration at different time points. Cells from CBA mice showed similar frequency of chromosome aberration at early and late time points, while cells from C57 mice showed very different chromosome aberration rate at early and late time points. Our results suggest that relative biological effectiveness (RBE) of radiation are different for different radiation sources, for different cell types, and for the same cell type with different genetic background at different times after radiation exposure. Caution must be taken in using RBE value to estimate biological effects from radiation exposure.

Lu, Tao↗

Improvements to Lunar BRDF-Corrected Nighttime Satellite Imagery: Uses and Applications

Observations made by the VIIRS day/night band (DNB) provide daily, nighttime measurements to monitor Earth surface processes.However, these observations are impacted by variations in reflected solar radiation on the moon's surface. As the moon transitions from new to full phase, increasing radiance is reflected to the Earth's surface and contributes additional reflected moonlight from clouds and land surface, in addition to emissions from other light sources observed by the DNB. The introduction of a bi-directional reflectance distribution function (BRDF) algorithm serves to remove these lunar variations and normalize observed radiances. Provided by the Terrestrial Information Systems Laboratory at Goddard Space Flight Center, a 1 km gridded lunar BRDF-corrected DNB product and VIIRS cloud mask can be used for a multitude of nighttime applications without influence from the moon. Such applications include the detection of power outages following severe weather events using pre-and post-event DNB imagery, as well as the identification of boat features to curtail illegal fishing practices. This presentation will provide context on the importance of the lunar BRDF correction algorithm and explore the aforementioned uses of this improved DNB product for applied science applications.

NWS↗

State of the Carbon Cycle - Consequences of Rising Atmospheric CO2

The rise of atmospheric CO2, largely attributable to human activity through fossil fuel emissions and land-use change, has been dampened by carbon uptake by the ocean and terrestrial biosphere. We outline the consequences of this carbon uptake as direct and indirect effects on terrestrial and oceanic systems and processes for different regions of North America and the globe. We assess the capacity of these systems to continue to act as carbon sinks. Rising CO2 has decreased seawater pH; this process of ocean acidification has impacted some marine species and altered fundamental ecosystem processes with further effects likely. In terrestrial ecosystems, increased atmospheric CO2 causes enhanced photosynthesis, net primary production, and increased water-use efficiency. Rising CO2 may change vegetation composition and carbon storage, and widespread increases in water use efficiency likely influence terrestrial hydrology and biogeochemical cycling. Consequences for human populations include changes to ecosystem services including cultural activities surrounding land use, agricultural or harvesting practices. Commercial fish stocks have been impacted and crop production yields have been changed as a result of rising CO2. Ocean and terrestrial effects are contingent on, and feedback to, global climate change. Warming and modified precipitation regimes impact a variety of ecosystem processes, and the combination of climate change and rising CO2 contributes considerable uncertainty to forecasting carbon sink capacity in the ocean and on land. Disturbance regime (fire and insects) are modified with increased temperatures. Fire frequency and intensity increase, and insect lifecycles are disrupted as temperatures move out of historical norms. Changes in disturbance patterns modulate the effects of rising CO2 depending on ecosystem type, disturbance frequency, and magnitude of events. We discuss management strategies designed to limit the rise of atmospheric CO2 and reduce uncertainty in forecasts of decadal and centennial feedbacks of rising atmospheric CO2 on carbon storage.

Carbon Cycle↗

Applications of VIC for Climate Land Cover Change Imapacts

Study focuses on the Lower Mekong Basin (LMB), the LMB is an economically and ecologically important region: (1) One of the largest exporters of rice and fish products, (2) Within top three most biodiverse river basins in the world. Natural climate variability plays an important role in water supply within the region: (1) Short-term climate variability (ENSO, MJO), (2) Long-term climate variability (climate change). Projections of climate change show there will be a decrease in water availability world wide which has implications for food security and ecology. Additional studies show there may be socioeconomic turmoil due to water wars and food security in developing regions such as the Mekong Basin. Southeast Asia has experienced major changes in land use and land cover from 1980 – 2000. Major economic reforms resulting in shift from subsistence farming to market-based agricultural production. Changes in land cover continue to occur which have an important role within the land surface aspect of hydrology.

remote sensing↗

Fluid Lensing and Applications to Remote Sensing of Aquatic Environments

The use of fluid lensing technology on UAVs is presented as a novel means for 3D imaging of aquatic ecosystems from above the water's surface at the centimeter scale. Preliminary results are presented from airborne fluid lensing campaigns conducted over the coral reefs of Ofu Island, American Samoa (2013) and the stromatolite reefs of Shark Bay, Western Australia (2014), covering a combined area of 15km2. These reef ecosystems were revealed with centimetre-scale 2D resolution, and an accompanying 3D bathymetry model was derived using fluid lensing, Structure from Motion and UAV position data. Data products were validated from in-situ survey methods including underwater calibration targets, depth measurements and millimetre-scale high-dynamic range gigapixel photogrammetry. Fluid lensing is an experimental technology that uses water transmitting wavelengths to passively image underwater objects at high-resolution by exploiting time-varying optical lensing events caused by surface waves. Fluid lensing data are captured from low-altitude, cost-effective electric UAVs to achieve multispectral imagery and bathymetry models at the centimetre scale over regional areas. As a passive system, fluid lensing is presently limited by signal-to-noise ratio and water column inherent optical properties to approximately 10 m depth over visible wavelengths in clear waters. The datasets derived from fluid lensing present the first centimetre-scale images of a reef acquired from above the ocean surface, without wave distortion. The 3D multispectral data distinguish coral, fish and invertebrates in American Samoa, and reveal previously undocumented, morphologically distinct, stromatolite structures in Shark Bay. These findings suggest fluid lensing and multirotor electric drones represent a promising advance in the remote sensing of aquatic environments at the centimetre scale, or 'reef scale' relevant to the conservation of reef ecosystems. Pending further development and validation of fluid lensing methods, these technologies present a solution for large-scale 3D surveys of shallow aquatic habitats with centimetre-scale spatial resolution and hourly temporal sampling.

Aquatic↗

Computational Model Prediction and Biological Validation Using Simplified Mixed Field Exposures for the Development of a GCR Reference Field

The yield of chromosomal aberrations has been shown to increase in the lymphocytes of astronauts after long-duration missions of several months in space. Chromosome exchanges, especially translocations, are positively correlated with many cancers and are therefore a potential biomarker of cancer risk associated with radiation exposure. Although extensive studies have been carried out on the induction of chromosomal aberrations by low- and high-LET radiation in human lymphocytes, fibroblasts, and epithelial cells exposed in vitro, there is a lack of data on chromosome aberrations induced by low dose-rate chronic exposure and mixed field beams such as those expected in space. Chromosome aberration studies at NSRL will provide the biological validation needed to extend the computational models over a broader range of experimental conditions (more complicated mixed fields leading up to the galactic cosmic rays (GCR) simulator), helping to reduce uncertainties in radiation quality effects and dose-rate dependence in cancer risk models. These models can then be used to answer some of the open questions regarding requirements for a full GCR reference field, including particle type and number, energy, dose rate, and delivery order. In this study, we designed a simplified mixed field beam with a combination of proton, helium, oxygen, and iron ions with shielding or proton, helium, oxygen, and titanium without shielding. Human fibroblasts cells were irradiated with these mixed field beam as well as each single beam with acute and chronic dose rate, and chromosome aberrations (CA) were measured with 3-color fluorescent in situ hybridization (FISH) chromosome painting methods. Frequency and type of CA induced with acute dose rate and chronic dose rates with single and mixed field beam will be discussed. A computational chromosome and radiation-induced DNA damage model, BDSTRACKS (Biological Damage by Stochastic Tracks), was updated to simulate various types of CA induced by acute exposures of the mixed field beams used for the experiments. The chromosomes were simulated by a polymer random walk algorithm with restrictions to their respective domains in the nucleus [1]. The stochastic dose to the nucleus was calculated with the code RITRACKS [2]. Irradiation of a target volume by a mixed field of ions was implemented within RITRACKs, and the fields of ions can be delivered over specific periods of time, allowing the simulation of dose-rate effects. Similarly, particles of various types and energies extracted from a pre-calculated spectra of galactic cosmic rays (GCR) can be used in RITRACKS. The number and spatial location of DSBs (DNA double-strand breaks) were calculated in BDSTRACKS using the simulated chromosomes and local (voxel) dose. Assuming that DSBs led to chromosome breaks, and simulating the rejoining of damaged chromosomes occurring during repair, BDSTRACKS produces the yield of various types of chromosome aberrations as a function of time (only final yields are presented). A comparison between experimental and simulation results will be shown.

Hada, M.↗

Discreditation of Bobdownsite and the Establishment of Criteria for the Identification of Minerals with Essential Monofluorophosphate (PO3F2-)

Bobdownsite, IMA number 2008-037, was approved as a new mineral by the Commission on New Minerals, Nomenclature and Classification (CNMNC) as the fluorine endmember of the mineral whitlockite. The type locality of bobdownsite is in Big Fish River, Yukon Canada, and bobdownsite was reported to be the first naturally occurring mineral with essential monofluorophosphate (PO3F2-). The type specimen of bobdownsite has been reinvestigated by electron probe microanalysis (EPMA), and our data indicate that fluorine abundances are below detection in the mineral. In addition, we conducted detailed analysis of bobdownsite from the type locality by gas chromatography isotope ratio mass spectrometry, Raman spectroscopy, EPMA, and NMR spectroscopy. These data were compared with previously published data on synthetic monofluorophosphate salts. Collectively, these data indicate that bobdownsite is indistinguishable from whitlockite with a composition along the whitlockite-merrillite solid solution. Bobdownsite is therefore discredited as a valid mineral species. An additional mineral, krásnoite, has been purported to have monofluorophosphate components in its structure, but reexamination of those data indicate that F- in krásnoite forms bonds with Al, similar to OH- bonded to Al in perhamite. Consequently, krásnoite also lacks monofluorophosphate groups, and there are currently no valid mineral species with monofluorophosphate in their structure. We recommend that any future reports of new minerals that contain essential monofluorophosphate anions be vetted by abundance measurements of fluorine, vibrational spectroscopy (both Raman and FTIR), and where paramagnetic components are permissibly low, NMR spectroscopy. Furthermore, we emphasize the importance of using synthetic compounds containing monofluorophosphate anions as a point of comparison in the identification of minerals with essential monofluorophosphate. Structural data that yield satisfactory P-F bond lengths determined by X-ray crystallography, coupled with direct chemical analyses of fluorine in a material do not constitute sufficient evidence alone to identify a new mineral with essential monofluorophosphate anions.

McCubbin, Francis M.↗

Biology IS the Technology: the Microbial Ecology of Space Food Production and the Power of Aquaponics as a Learning Tool

To accomplish the objective of human missions to Mars and/or the long-term colonization of the moon, bioregenerative life support systems and food production systems will be absolutely necessary. Microbes are an essential and unavoidable component of these systems. In fact, these systems are driven by complex microbial communities about which we know very little, a glaring strategic knowledge gap in our ability to support extended human exploration in closed systems. Our laboratory has been working to use molecular ecological methods, including nanopore sequencing technology already deployed on the International Space Station, to understand the microbes in food production systems on Earth. Our ultimate goal is to inform the implementation of food production systems off-world. To date, we have sampled and sequenced the microbiomes of aquaponics systems, hydroponics systems, and fish ponds. Our results have revealed that the microbial communities in these systems are extremely diverse, and highly variable between systems. Along the way, we have discovered the power of aquaponics systems as teaching tools, and the capacity of students to perform high quality citizen science. By designing, constructing, and operating aquaponics systems, students better understand the role of microbes in the cycling of the elements in natural ecosystems, and in the human built environment. In partnership with schools and colleges, contributing new knowledge as citizen scientists, we are now exploring the relationships between the functioning of these systems and their microbial flora.

Bebout, Brad↗

Food Quality and Phytoplankton Community Composition in San Francisco Bay using Imaging Spectroscopy Data from the California HyspIRI Airborne Campaign

The San Francisco Bay (SFB) is the largest estuary on the west coast of the United States. It is an important transition zone between marine, freshwater, and inland terrestrial watersheds. The SFB is an important region for the cycling of nutrients and pollutants and it supports nurseries of ecologically and commercially important fisheries, including some threatened species. Phytoplankton community structure influences food web dynamics, and the taxonomy of the phytoplankton may be more important in determining primary food quality than environmental factors. As such, estimating food quality from phytoplankton community composition can be a robust tool to understand trophic transfer of energy. Recent work explores phytoplankton food quality in SFB through the use of microscopy and phytoplankton chemotaxonomy to evaluate how changes in phytoplankton composition may have influenced the recent trophic collapse of pelagic fishes in the northern part of the SFB. The objective of this study is to determine if the approach can also be applied to imaging spectroscopy data in order to quantify phytoplankton food quality from space. Imaging spectroscopy data of SFB from the Airborne VisibleInfrared Imaging Spectrometer (AVIRIS) was collected during the Hyperspectral Infrared (HyspIRI) Airborne Campaign in California (2013 2015) and used in this study. Estimates of ocean chlorophyll and phytoplankton community structure were determined using standard ocean chlorophyll algorithms and the PHYtoplankton Detection with Optics (PHYDOTax) algorithms. These were validated using in situ observations of phytoplankton composition using microscopic cell counts and phytoplankton chemotaxonomy from the US Geological Surveys ship surveys of the SFB. The findings from this study may inform the use of future high spectral resolution satellite sensors with the spatial resolution appropriate for coastal systems (e.g., HyspIRI) to assess food quality from space.

HyspIRI↗

Prioritizing Aquatic Science and Applications Needs in the Chesapeake Bay for a Space-Borne Hyperspectral Mission

The Chesapeake Bay is the largest estuary in North America, benefiting a growing population through its ecosystem services, fishing, recreations, and transportation routes. Studies indicate the health of the Bay as seen some improvement in recent years, yet threats to its health persist (e.g. warming, pollution nutrient run-off). Increasing human activities in coastal regions requires constant vigilance by agencies managing water quality, to ensure the safety of the population. Since April 2018, an interagency working group has been meeting monthly and a daylong workshop was convened with science and applications stakeholders around the overall theme of monitoring water quality from space. Current ocean color images indicate bloom locations used to guide in situ sampling efforts, despite limited spatial, spectral and temporal resolution. High resolution hyperspectral remote sensing provides a potential opportunity to measure additional indicators of ecological health and water quality. Assessing the needs of the aquatic user community around the Chesapeake Bay will inform science and applications recommendations during the current architecture study for a Surface Biology and Geology (SBG) Mission, as well as future scoping studies of other coastal and inland water bodies.

Schollaert Uz, Stephanie↗

A Predictive Model for Lake Chad Total Surface Water Area Using Remotely Sensed and Modeled Hydrological and Meteorological Parameters and Multivariate Regression Analysis

Lake Chad is an endorheic lake in the Sahel region of Africa at the southern edge of the Sahara Desert. The lake, which is well known for its dramatic decrease in surface area during the 1970s and 1980s, experiences an annual flood resulting in a maximum total surface water area generally during February or March, though sometimes earlier or later. People along the shores of Lake Chad make their living fishing, farming, and raising livestock and have a vested interest in knowing when and how extensive the annual flooding will be, particularly those practicing recession farming in which the fertile ground of previously flooded area is used for planting crops. In this study, the authors investigate the relationship between lake and basin parameters, including rainfall, basin evapotranspiration, lake evapotranspiration, lake elevation, total surface water area, and the previous year’s total surface water area, and develop equations for each dry season month (except November) linking total surface water area to the other parameters. The resulting equations allow the user to estimate the December average monthly total surface water area of the lake in late November, and to make the estimates for January to May in early December. Based on the results of a Leave One Out Cross Validation analysis, the equations for lake area are estimated to have an average absolute error ranging from 5.3 percent (for February estimates) to 7.6 percent (for May estimates).

Lake Chad↗

Flight Demonstration of Novel Atmospheric Satellite Concept

The major focus of the Phase II effort described herein is to develop and demonstrate an aircraft capable of autonomously sailing (i.e., to cruise without propulsion or external assistance), and thereby prove that the dual-aircraft platform (DAP) atmospheric satellite concept is potentially viable. This sailing mode of flight was identified as the number-1 enabling technology required for the stratospheric DAP concept (also known as Stratosat) in the NIAC (NASA Innovative Advanced Concept) Phase I effort. No scientific demonstration of this technology has ever been done or documented to our knowledge. This report describes efforts to take a major step towards the sailing mode of flight capability using a single aircraft connected by cable to a moving ground vehicle which uses sufficient crosswind to cruise without propulsion while "pulling" the ground vehicle forward (i.e., without external assistance). The development of a prototype aircraft is described in terms of novel and key hardware and software elements. A specialized prototype aircraft is described, including a novel cable release mechanism, novel "lateron" control surfaces, and a highly-accurate onboard wind measurement system. Additionally, a novel means to safely connect the aircraft to the moving ground vehicle is described involving a fishing rod/reel and integrated load cell. All of these devices were designed and developed in-house and validated in flight testing. Software is developed to provide look-up tables that give the flight condition targets (i.e., 3-D position relative to ground vehicle, forward speed, aircraft orientation, etc.), based on current wind speed and direction. These tables are successfully validated in flight simulation and used onboard the aircraft. High fidelity analysis of the aircraft aerodynamics are described - required to produce accurate target sailing flight conditions. A novel wind tunnel measurement technique is developed to accurately assess the aerodynamics of the ultra-thin cable. A new specialized flight simulator is described which is utilized to develop and verify the flight software required onboard the aircraft, and to support training of pilots for flying the aircraft while tethered to a ground vehicle. The DAP flight simulator was developed within the Matlab-Simulink framework and included detailed treatment of aircraft/cable aerodynamics, cable dynamics, experimentally-derived propeller-motor thrust curves, actuator responsiveness, and realistic air turbulence. The specialized formation flight controller algorithm, developed using this flight simulator, and onboard the aircraft is described. Finally, a novel auto-tuning software is described and verified within the flight simulator that is shown to refine the sailing flight condition targets during flight using an optimization technique involving doublet maneuvers. Virtual flights using the auto-tuning software indicate that the prototype aircraft should be able to reach and hold sailing conditions despite moderate levels of turbulence provided there is sufficient mean wind available. An overview of the flight testing program is provided. Hundreds of short flights were conducted, primarily using a “dead” short runway at Deland Municipal Airport which permitted use of a moving ground vehicle. Additional flight tests at Space Florida’s Shuttle Landing Facility are also described. First year results from these tests in which the aircraft is controlled manually, demonstrated that excessive flight testing would be required for a pilot to learn to sail with visual cues. However, second year results from autonomous flight these tests included successful demonstration of the closed-loop autonomous formation flight capability (i.e., autonomously determine, reach, and hold the required 3-D location relative to the ground vehicle required for sailing). The next step of using the auto-tune software to autonomously refine the aircraft orientation targets to finally achieve sailing remains the primary goal of future work.

Flight↗

Bloom Modeling and Prediction of the Harmful Algae Alexandrium in Bellingham Bay, WA

Bellingham, Washington is located near the American-Canadian border in the northwestern part of Washington state, and is bordered on its west side by the Lummi reservation. Between Lummi and Bellingham lies Bellingham bay, which has a history of harmful algae related closures dating back to 1978. The subject of this work is a genus of dinoflagellates: Alexandrium, within which many species have the capacity to produce a suite of toxins known as saxitoxin. These toxins bioaccumulate in bivalves, which in turn cause paralytic shellfish poisoning in marine consumers (mammals, birds, and fish), including humans. Symptoms in humans can range from tingling and numbness to difficulty or inability to breathe, resulting in death. Because of the longstanding history of shellfish gathering among Salish tribes and the fact that Washington is the leading U.S. producer of farmed bivalves, harmful algae blooms impact both native and non-native peoples living in the Salish Sea area negatively. The objective of this work is to identify factors which influence Alexandrium blooms in Bellingham Bay, as well as predict Alexandrium blooms in the future. The method of doing so involves two processes: an initial statistical modeling phase to find in situ and remote sensing observations correlated to bloom density (including, but not limited to: water temperature, chlorophyll-a, salinity, color dissolved organic matter, and discharge rate of local rivers), followed by use of that data as a training set for a recursive neural network. This predictive capacity may inform future closures, help ensure the safety of shellfish consumers, and act as a baseline for future modeling efforts in the region.

Harmful↗

2018 NISAR Applications Workshop: Wetlands; Workshop Report

Wetland ecosystems are a critical part of our natural environment, providing socioeconomic benefits to human communities and habitats to a rich diversity of plant and animal life. Socioeconomic benefits include improved water quality, flood control, foods, shoreline stabilization, groundwater recharge, and recreational opportunities. Wetlands also have a major role as carbon sinks and sources through processes that are influenced by the duration and timing of soil saturation and inundation. Thus, carbon and water cycle models must take into account wetland extent and seasonal patterns of wetland inundation. The joint NASA, US Geological Survey (USGS) and Fish and Wildlife Service (FWS) workshop focused on advancing wetland applications of the spaceborne NASA-ISRO Synthetic Aperture Radar (SAR) mission (NISAR), a jointly developed satellite between NASA and the Indian Space Research Organisation (ISRO) expected for launch early 2022. Participants from 15 national and international organizations --including US Federal Agencies, nonprofits, academics, and the private sector-- had been identified as key-players in facilitating integration of Earth Observations into decision support workflows. Discussions were held over two and a half days to convey the knowledge and measurement needs of the wetlands community and discuss the delivery of relevant geospatial products that could be derived from NISAR data. While the community typically characterizes wetlands by their hydrological process, vegetation and soil types, a central defining characteristic is that a wetland is a land area inundated or saturated in the rootzone for at least 2 weeks of the average vegetation growing season.

FWS↗

TPSAS-NF1676L-31944-DND

The Plum Island Estuary (PIE) in Massachusetts is New England’s largest salt marsh. This dynamic ecosystem plays an important role in the surrounding communities by providing ecosystem and social services, acting as a center for education, research, and recreation. However, marshes around the world are threatened by sea level rise. As the equilibrium between sediment supply, erosion rates, and vegetation growth is unbalanced by rising waters, marshes are liable to recede, or even drown. Because sediment is so crucial, researchers use sediment budgets as a metric to assess Plum Island Estuary’s vulnerability to rising seas; however, established data collection methods are limited in scope. The project employed five years of imagery from Landsat 8 Operational Land Imager (OLI) and three years of imagery from Sentinel-2 Multispectral Instrument (MSI), in conjunction with in situ data, to generate and refine a local algorithm that derives suspended sediment concentration from remote sensing reflectance. This information was applied to a flux model to analyze transport patterns and possible sediment sources, particularly the Merrimack River. The use of remote sensing techniques will provide our partners at the US Geological Survey, US Fish and Wildlife Service, and Plum Island Ecosystems Long Term Ecological Research Network with higher spatial and temporal resolution data, which will allow for the development of more effective management practices.

Sydney Neugebauer↗

Great Lakes Water Resources II: A Google Earth Engine Tool to Automate Wetland Mapping Using Optical and Radar Satellite Sensors in the Great Lakes Basin for Wetland Management and Monitoring

The Great Lakes Basin is one of the world’s largest freshwater ecosystems. The Basin harbors over 200,000 acres of wetlands. These wetlands provide a variety of environmental, ecological, and recreational functions to over 30 million people in the region. Some of these functions include improving water quality, mitigating flood impacts, providing wildlife habitat, and housing recreational activities. However, due to anthropogenic activities, habitat conversion and degradation threaten to disrupt or destroy remaining wetland ecosystems. Maps of wetland distribution based on ground surveys are costly and labor-intensive, prohibiting timely evaluations of wetland loss and gain. The Great Lakes Water Resources II team at the NASA Jet Propulsion Laboratory developed the Wetlands Extent Tool 2.0 (WET 2.0) in Google Earth Engine to automate mapping of wetland distribution in the Great Lakes Basin. The team partnered with the US Fish and Wildlife Service (USFWS), Environmental Protection Agency (EPA), Minnesota Department of Natural Resources (MDNR), the National Oceanic and Atmospheric Administration (NOAA), and Ducks Unlimited (DU). WET 2.0 incorporates Landsat 8 Operational Land Imager (OLI), Sentinel-1 C-band Synthetic Aperture Radar (C-SAR), and Sentinel-2 Multispectral Instrument (MSI) satellite data. WET 2.0 is trained to classify anywhere in the Great Lakes Basin. Utilizing a Random Forest classifier, WET 2.0 is capable of automatically mapping wetland extent in the entire Great Lakes Basin, achieving a mean overall accuracy of 80.12% when tested in Michigan. Findings and maps produced in WET 2.0 will enable our partners to identify areas of ecosystem degradation and wetland destruction in order to enact environmental practices and policy initiatives to maintain environmental and economic health in the area.

Water Resources↗

Colorado & New Mexico Water Resources: Mapping Wetland and Riparian Areas to Support Rio Grande Cutthroat Trout Habitat Restoration

Over the last century, the Rio Grande cutthroat trout (Oncorhynchus clarki virginalis; RGCT) population has declined significantly due to habitat loss, competition, and hybridization with non-native trout species; the species currently occupies roughly 11% of its historic habitat. Conservation efforts led by governmental and private actors have succeeded in increasing RGCT populations since the early 2000s. Vermejo Park Ranch, a privately owned 560,000-acre property, partnered with US Fish and Wildlife Service and Colorado Parks and Wildlife (CPW) to begin the largest native trout restoration initiative in North America. Since 2002, these efforts have included wetland and riparian area restoration and RGCT reintroduction. Current restoration efforts focus on restoring the Costilla Creek Watershed to provide cool water temperatures, improve water quality, and create the necessary habitat requirements for the trout species. To guide these restoration efforts, the Colorado – Fort Collins NASA DEVELOP team produced maps to locate and characterize wetland and riparian areas in the Costilla Creek watershed. The team utilized NASA data from Landsat 8 Operational Land Imager (OLI), as well as Sentinel-2 MultiSpectral Instrument (MSI), Sentinel-1 Synthetic Aperture Radar (SAR), and additional ancillary data for May 2016 to October 2019. In order to produce probability maps of wetland presence, the team used the Software for Assisted Habitat Modeling (SAHM) incorporating predictor variables generated from topographic indices, spectral indices, and radar. The resulting maps allowed Vermejo Ranch and CPW to determine the extent of possible RGCT habitat and identify where habitat restoration efforts may be needed.

Water Resources↗

Colorado & New Mexico Water Resources: Mapping Wetland and Riparian Areas to Support Rio Grande Cutthroat Trout Habitat Restoration

Over the last century, the Rio Grande cutthroat trout (Oncorhynchus clarki virginalis; RGCT) population has declined significantly due to habitat loss, competition, and hybridization with non-native trout species; the species currently occupies roughly 11% of its historic habitat. Conservation efforts led by governmental and private actors have succeeded in increasing RGCT populations since the early 2000s. Vermejo Park Ranch, a privately owned 560,000-acre property, partnered with US Fish and Wildlife Service and Colorado Parks and Wildlife (CPW) to begin the largest native trout restoration initiative in North America. Since 2002, these efforts have included wetland and riparian area restoration and RGCT reintroduction. Current restoration efforts focus on restoring the Costilla Creek Watershed to provide cool water temperatures, improve water quality, and create the necessary habitat requirements for the trout species. To guide these restoration efforts, the Colorado –Fort Collins NASA DEVELOP team produced maps to locate and characterize wetland and riparian areas in the Costilla Creek watershed. The team utilized NASA data from Landsat 8 Operational Land Imager, and Landsat 5 Thematic Mapper in conjunction with Sentinel-2 MultiSpectral Instrument, Sentinel-1 Synthetic Aperture Radar, and additional ancillary data for May 2016 to October 2019. In order to produce probability maps of wetland presence, the team used the Software for Assisted Habitat Modeling (SAHM) incorporating predictor variables generated from topographic indices, spectral indices, and radar. The resulting maps allowed Vermejo Ranch and CPW to determine the extent of possible RGCT habitat and identify where habitat restoration efforts are needed.

Health & Air Quality↗

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