Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “FILAMENTS”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,801 records · Page 100

Tracing the Magnetic Field in Orion A

We use extensive 350 micron polarimetry and continuum maps obtained with Hertz and SHARC II along with HCN and HCO(sup +) spectroscopic data to trace the orientation of the magnetic field in the Orion A star-forming region. Using the polarimetry data, we find that the direction of the projection of the magnetic field in the plane of the sky relative to the orientation of the integral-shaped filament varies considerably as one moves from north to south. While in IRAS 05327-0457 and OMC-3 MMS 1-6 the projection of the field is primarily perpendicular to the filament it becomes better aligned with it at OMC-3 MMS 8-9 and well aligned with it at OMC-2 FIR 6. The OMC-2 FIR 4 cloud, located between the last two, is a peculiar object where we find almost no polarization. There is a relatively sharp boundary within its core where two adjacent regions exhibiting differing polarization angles merge. The projected angle of the field is more complicated in OMC-1 where it exhibits smooth variations in its orientation across the face of this massive complex. We also note that while the relative orientation of the projected angle of the magnetic field to the filament varies significantly in the OMC-3 and OMC-2 regions, its orientation relative to a fixed position on the sky shows much more stability. This suggests that, perhaps, the orientation of the field is relatively unaffected by the mass condensations present in these parts of the molecular cloud. By combining the polarimetry and spectroscopic data we were able to measure a set of average d u e s for the inclination angle of the magnetic field relative to the line of sight. We find that the field is oriented quite close to the plane of the sky in most places. More precisely, the inclination of the magnetic field is approx. = 73 deg around OMC-3 MMS 6, approx. = 74 deg at OMC-3 MMS 8-9, approx. = 80 deg at OMC-2 FIR 4, approx. = 65 deg in the northeastern part of OMC-1, and approx. = 49 deg in the Bas. The small difference in the inclination of the field between OMC-3 and OMC-2 seems to strengthen the idea that the orientation of the magnetic field is relatively unaffected by the agglomeration of matter located in these regions. We also present polarimetry data for the OMC-4 region located some 13 min. south of OMC-1.

Dowell, C. Darren↗

CME Eruption Onset Observations from EIT and SXT

Why CMEs erupt is a major outstanding puzzle of solar physics. Signatures observable at the earliest stages of eruption onset may hold precious clues about the onset mechanism. We present observations in EUV from SOHO/EIT and in soft X-rays from Yohkoh/SXT of the re-eruption and eruption phases of CME expulsion, along with the eruption's magnetic setting found from SOHO/MDI magnetograms. Most of our events involve clearly-observable filament eruptions and multiple neutral lines, and we use the magnetic settings and motions of the filaments to help infer the geometry and behavior of the associated erupting magnetic fields. Pre-eruption and early-eruption signatures include a relatively slow filament rise prior to eruption, and intensity "dimmings" and brightenings, both in the immediate neighborhood of the "core" (location of greatest magnetic shear) of the erupting fields and at locations remote from the core. These signatures and their relative timings place observational constraints on eruption mechanisms; our recent work has focused on implications for the so-called "tether cutting" and "breakout" models, but the same observational constraints are applicable to any model.

Sterling, A. C.↗

Overexpression of neurofilament H disrupts normal cell structure and function

Studying exogenously expressed tagged proteins in live cells has become a standard technique for evaluating protein distribution and function. Typically, expression levels of experimentally introduced proteins are not regulated, and high levels are often preferred to facilitate detection. However, overexpression of many proteins leads to mislocalization and pathologies. Therefore, for normative studies, moderate levels of expression may be more suitable. To understand better the dynamics of intermediate filament formation, transport, and stability in a healthy, living cell, we inserted neurofilament heavy chain (NFH)-green fluorescent protein (GFP) fusion constructs in adenoviral vectors with tetracycline (tet)-regulated promoters. This system allows for turning on or off the synthesis of NFH-GFP at a selected time, for a defined period, in a dose-dependent manner. We used this inducible system for live cell imaging of changes in filament structure and cell shape, motility, and transport associated with increasing NFH-GFP expression. Cells with low to intermediate levels of NFH-GFP were structurally and functionally similar to neighboring, nonexpressing cells. In contrast, overexpression led to pathological alterations in both filament organization and cell function. Copyright 2002 Wiley-Liss, Inc.

Non-NASA Center↗

Bacterial mineralization patterns in basaltic aquifers: implications for possible life in martian meteorite ALH84001

To explore the formation and preservation of biogenic features in igneous rocks, we have examined the organisms in experimental basaltic microcosms using scanning and transmission electron microscopy. Four types of microorganisms were recognized on the basis of size, morphology, and chemical composition. Some of the organisms mineralized rapidly, whereas others show no evidence of mineralization. Many mineralized cells are hollow and do not contain evidence of microstructure. Filaments, either attached or no longer attached to organisms, are common. Unattached filaments are mineralized and are most likely bacterial appendages (e.g., prosthecae). Features similar in size and morphology to unattached, mineralized filaments are recognized in martian meteorite ALH84001.

NASA Discipline Exobiology↗

Technical advance: identification of plant actin-binding proteins by F-actin affinity chromatography

Proteins that interact with the actin cytoskeleton often modulate the dynamics or organization of the cytoskeleton or use the cytoskeleton to control their localization. In plants, very few actin-binding proteins have been identified and most are thought to modulate cytoskeleton function. To identify actin-binding proteins that are unique to plants, the development of new biochemical procedures will be critical. Affinity columns using actin monomers (globular actin, G-actin) or actin filaments (filamentous actin, F-actin) have been used to identify actin-binding proteins from a wide variety of organisms. Monomeric actin from zucchini (Cucurbita pepo L.) hypocotyl tissue was purified to electrophoretic homogeneity and shown to be native and competent for polymerization to actin filaments. G-actin, F-actin and bovine serum albumin affinity columns were prepared and used to separate samples enriched in either soluble or membrane-associated actin-binding proteins. Extracts of soluble actin-binding proteins yield distinct patterns when eluted from the G-actin and F-actin columns, respectively, leading to the identification of a putative F-actin-binding protein of approximately 40 kDa. When plasma membrane-associated proteins were applied to these columns, two abundant polypeptides eluted selectively from the F-actin column and cross-reacted with antiserum against pea annexins. Additionally, a protein that binds auxin transport inhibitors, the naphthylphthalamic acid binding protein, which has been previously suggested to associate with the actin cytoskeleton, was eluted in a single peak from the F-actin column. These experiments provide a new approach that may help to identify novel actin-binding proteins from plants.

Non-NASA Center↗

Physiology of a microgravity environment invited review: microgravity and skeletal muscle

Spaceflight (SF) has been shown to cause skeletal muscle atrophy; a loss in force and power; and, in the first few weeks, a preferential atrophy of extensors over flexors. The atrophy primarily results from a reduced protein synthesis that is likely triggered by the removal of the antigravity load. Contractile proteins are lost out of proportion to other cellular proteins, and the actin thin filament is lost disproportionately to the myosin thick filament. The decline in contractile protein explains the decrease in force per cross-sectional area, whereas the thin-filament loss may explain the observed postflight increase in the maximal velocity of shortening in the type I and IIa fiber types. Importantly, the microgravity-induced decline in peak power is partially offset by the increased fiber velocity. Muscle velocity is further increased by the microgravity-induced expression of fast-type myosin isozymes in slow fibers (hybrid I/II fibers) and by the increased expression of fast type II fiber types. SF increases the susceptibility of skeletal muscle to damage, with the actual damage elicited during postflight reloading. Evidence in rats indicates that SF increases fatigability and reduces the capacity for fat oxidation in skeletal muscles. Future studies will be required to establish the cellular and molecular mechanisms of the SF-induced muscle atrophy and functional loss and to develop effective exercise countermeasures.

short duration↗

[The growth movements of moss protonemata under clinostatic and microgravity conditions]

Populations of dark-grown protonemata of moss Ceratodon purpureus wt-4 (Germany) and wt-U (Ukraine) were rotated on clinostat or flown in space (experiment "Protonema" aboard Bion-11, December 24, 1996-January 7, 1997) to determine the effects of altered gravity on orientation of protonemata growing filaments. Protonemata had been cultivated 8 days in vertical stationary position at dark to be transported to microgravity or placed in clinostat for the period of 14 days. In the ground control, protonemata demonstrated the negatively gravitropic growth (straight upwards in a bundle of compact filaments). The horizontal or circular rotation in clinostat and exposure to microgravity made filaments grow every each way within the substrate plane but with an apparent trend to rightward curling resulting in "spiral galaxies".

NASA Discipline Plant Biology↗

X-ray and EUV Observations of CME Eruption Onset

Why Coronal Mass Ejections (CMEs) erupt is a major outstanding puzzle of solar physics. Signatures observable at the earliest stages of eruption onset may hold precious clues about the onset mechanism. We present observations from SOHO/EIT and from TRACE in EUV, and from Yohkoh/SXT in soft X-rays of the pre-eruption and eruption phases of CME expulsion, along with the eruption's magnetic setting found from SOHO/MDI magnetograms. Most of our events involve clearly-observable filament eruptions and multiple neutral lines, and we use the magnetic settings and motions of the filaments to help infer the geometry and behavior of the associated erupting magnetic fields. Pre-eruption and early-eruption signatures include a relatively slow filament rise prior to eruption, and intensity "dimmings" and brightenings, both in the immediate neighborhood of the "core" (location of greatest magnetic shear) of the erupting fields and at locations remote from the core. These signatures and their relative timings place observational constraints on eruption mechanisms; our recent work has focused on implications for the so-called "tether cutting" and "breakout" models, but the same observational constraints are applicable to any model.

Sterling, A. C.↗

The Arthromitus stage of Bacillus cereus: intestinal symbionts of animals

In the guts of more than 25 species of arthropods we observed filaments containing refractile inclusions previously discovered and named "Arthromitus" in 1849 by Joseph Leidy [Leidy, J. (1849) Proc. Acad. Nat. Sci. Philadelphia 4, 225-233]. We cultivated these microbes from boiled intestines of 10 different species of surface-cleaned soil insects and isopod crustaceans. Literature review and these observations lead us to conclude that Arthromitus are spore-forming, variably motile, cultivable bacilli. As long rod-shaped bacteria, they lose their flagella, attach by fibers or fuzz to the intestinal epithelium, grow filamentously, and sporulate from their distal ends. When these organisms are incubated in culture, their life history stages are accelerated by light and inhibited by anoxia. Characterization of new Arthromitus isolates from digestive tracts of common sow bugs (Porcellio scaber), roaches (Gromphodorhina portentosa, Blaberus giganteus) and termites (Cryptotermes brevis, Kalotermes flavicollis) identifies these flagellated, spore-forming symbionts as a Bacillus sp. Complete sequencing of the 16S rRNA gene from four isolates (two sow bug, one hissing roach, one death's head roach) confirms these as the low-G+C Gram-positive eubacterium Bacillus cereus. We suggest that B. cereus and its close relatives, easily isolated from soil and grown on nutrient agar, enjoy filamentous growth in moist nutrient-rich intestines of healthy arthropods and similar habitats.

Non-NASA Center↗

Effect of 17 days of bed rest on peak isometric force and unloaded shortening velocity of human soleus fibers

The purpose of this study was to examine the effect of prolonged bed rest (BR) on the peak isometric force (P0) and unloaded shortening velocity (V0) of single Ca(2+)-activated muscle fibers. Soleus muscle biopsies were obtained from eight adult males before and after 17 days of 6 degrees head-down BR. Chemically permeabilized single fiber segments were mounted between a force transducer and position motor, activated with saturating levels of Ca2+, and subjected to slack length steps. V0 was determined by plotting the time for force redevelopment vs. the slack step distance. Gel electrophoresis revealed that 96% of the pre- and 87% of the post-BR fibers studied expressed only the slow type I myosin heavy chain isoform. Fibers with diameter > 100 microns made up only 14% of this post-BR type I population compared with 33% of the pre-BR type I population. Consequently, the post-BR type I fibers (n = 147) were, on average, 5% smaller in diameter than the pre-BR type I fibers (n = 218) and produced 13% less absolute P0. BR had no overall effect on P0 per fiber cross-sectional area (P0/CSA), even though half of the subjects displayed a decline of 9-12% in P0/CSA after BR. Type I fiber V0 increased by an average of 34% with BR. Although the ratio of myosin light chain 3 to myosin light chain 2 also rose with BR, there was no correlation between this ratio and V0 for either the pre- or post-BR fibers. In separate fibers obtained from the original biopsies, quantitative electron microscopy revealed a 20-24% decrease in thin filament density, with no change in thick filament density. These results raise the possibility that alterations in the geometric relationships between thin and thick filaments may be at least partially responsible for the elevated V0 of the post-BR type I fibers.

STS-78 Shuttle Project↗

Miniature Instrumentation for SIPR (Subsurface Ice PRobe)

Ice coring has proved to be a valuable scientific tool for determining climate history on Earth. The goal of the SIPR project is to develop a simple extraterrestrial ice sampling method of comparable value to coring. The SIPR probe works by melting its way through glacial ice, pumping the melt water to the surface for analysis as it descends hundreds of meters. The specific geometry of the probe, along with size and power constraints, requires creative diagnostic instrumentation. A thin, vertically strung heated filament will provide continuous-level monitoring of water in down-hole containers. The filament has an appreciable temperature coefficient to resistance (TCR), so as water cools the wire, its resistance decreases. At a constant electrical current, the voltage across the filament varies linearly with water level.

Ostmo, Karl P.↗

The Strings of Eta Carina: The HST/STIS Spectra and [Ca II]

Long linear, filamentary ejecta, are found to move at very high velocity external to the Homunculus, the circumstellar hourglass-shaped ejecta surrounding Eta Carinae. The origin of the strings is a puzzle. As an example, the Weigelt Blobs have N at 10X solar and C, O at 0.01X solar abundance, along with He/H significantly enhanced. This abundance pattern is evidence for extreme CNO-processing. Similarly, the Strontium Filament has Ti/Ni at 100X solar, presumably due to the lack of oxygen to form Ti-oxide precipitates onto dust grains. We have obtained 2-D spectra with the HST/STIS of the Strontium Filament and a portion of a string. These deep spectral exposures, at moderate dispersion, span much of the near red spectral region from 5000 to 9000A. We have identified twelve emission lines in these spectra with proper velocities and spatial structure of this string and obtained line ratios for [Ca II] (7293/7325A) and [Fe Ill (7157/8619A) which are useful for determining physical conditions in this nebulosity. In an attempt to use the [Ca II] ratio to determine the physical parameters, and ultimately the abundances in the strings, we have constructed a statistical equilibrium model for Ca II , including radiative and collisional rates. These results incorporate our newly calculated atomic data for levels n = 3,4,5 and 6 configurations of Ca II. The aim is to compute the [Ca II] line ratios and use them as a diagnostic of the physical parameters. Using the [Fe II] ratio we find that for Te=10,000 K, the electron density is Ne approx.10(exp 6)/cu cm. We plan to use the [Ca II] ratio to confirm this result. Then, we will extend the use of this multilevel model Ca II atom to study the physical conditions of the Strontium filament where eight lines of Ca II, both allowed and forbidden, had been identified. With the physical conditions determined, we will be able to derive reliable estimates for the gas phase abundances in the strings.

Melendez, M. B.↗

Initiation of Solar Eruptions: Recent Observations and Implications for Theories

Solar eruptions involve the violent disruption of a system of magnetic field. Just how the field is destabilized and explodes to produce flares and coronal mass ejections (CMEs) is still being debated in the solar community. Here I discuss recent observational work into these questions by ourselves (me and my colleagues) and others. Our work has concentrated mainly on eruptions that include filaments. We use the filament motion early in the event as a tracer of the motion of the general erupting coronal field in and around the filament, since that field itself is hard to distinguish otherwise. Our main data sources are EUV images from SOHO/EIT and TRACE, soft Xray images from Yohkoh, and magnetograms from SOHO/MDI, supplemented with coronagraph images from SOHO/LASCO, hard X-ray data, and ground-based observations. We consider the observational findings in terms of three proposed eruption-initiation mechanisms: (i) runaway internal tether-cutting reconnection, (ii) slow external tether-cutting reconnection ("breakout"), and (iii) ideal MHD instability.

Sterling, A. C.↗

Dry powder process for preparing uni-tape prepreg from polymer powder coated filamentary towpregs

A process for preparing uni-tape prepreg from polymer powder coated filamentary towpregs is provided. A plurality of polymer powder coated filamentary towpregs are provided. The towpregs are collimated so that each towpreg is parallel. A material is applied to each side of the towpreg to form a sandwich. The sandwich is heated to a temperature wherein the polymer flows and intimately contacts the filaments and pressure is repeatedly applied perpendicularly to the sandwich with a longitudinal oscillating action wherein the filaments move apart and the polymer wets the filaments forming a uni-tape prepreg. The uni-tape prepreg is subsequently cooled.

Wilkinson, Steven P.↗

NanoSIMS Reveals New Structural and Elemental Signatures of Early Life

The young technology of NanoSIMS is unlocking new information from organic matter in ancient sediments. We have used this technique to characterize sub-micron scale element composition of Proterozoic organics that are clearly biogenic as a guide for interpreting problematic structures in terrestrial or extraterrestrial samples. We used the NanoSIMS 50 of the National Museum of Natural History in Paris to map carbon, nitrogen (as CN), and sulfur in organic structures from the approximately 0.8 Ga Bitter Springs Formation. We analyzed spheroidal and filamentous microfossils as well as organic laminae that appeared amorphous by optical and scanning electron microscopy. In clear-cut microfossils, a coincidence between optical images and NanoSIMS element maps suggests a biological origin for the mapped carbon, sulfur, and nitrogen; this conclusion is supported by high resolution NanoSIMS maps showing identical spatial distributions of C, CN and S. High resolution images also demonstrate distinctive nano structure of the filaments and spheroids. In the amorphous laminae, NanoSIMS reveals morphologies reminiscent of compressed microfossils. Distinct CN/C ratios of the spheroids, filaments, and laminae may reflect their biological precursors (cell walls, cyanobacterial sheaths, and microbial communities/biofilms, respectively). Similar amorphous laminae comprise a preponderance of the organic matter in many Precambrian deposits. Thus it is possible that NanoSIMS will provide fresh insight into a large body of previously uninterpretable material. Additionally, NanoSIMS analysis may establish new biosignatures that will be helpful for assessing the origin and biogenicity of controversial Archean structures and any organic materials that may occur in Martian or other extraterrestrial samples.

Oehler, Dorothy Z.↗

The Connection between the Corona and Chromosphere during a Multiple Event Observation

NOAA AR 10940 (~Jan 25 2007 - Feb 09 2007) rotated into view producing a CME and EIT wave, and followed by at least 27 B and C class flares as rotated across the disk. As it reached the west limb it proceeded to produce a sequence of jets and filament eruptions that were observed in unprecedented detail. I present observations from TRACE, EIT, HINODE XRT / SOT / EIS, RHESSI, GOES 12 and STEREO-A COR 1 to investigate part of the sequence of events that lead to a filament eruption and sympathetic jet at ~02:30 - 03:00 02/09/07. I concentrate on the topology changes and infer that external breakout reconnection was the mechanism that lead to the removal of overlying field and subsequent filament eruption I will also discuss the instrument development activities at MSFC.

Cirtain, Jonathan↗

Blowout Jets: Hinode X-Ray Jets that Don't Fit the Standard Model

Nearly half of all H-alpha macrospicules in polar coronal holes appear to be miniature filament eruptions. This suggests that there is a large class of X-ray jets in which the jet-base magnetic arcade undergoes a blowout eruption as in a CME, instead of remaining static as in most solar X-ray jets, the standard jets that fit the model advocated by Shibata. Along with a cartoon depicting the standard model, we present a cartoon depicting the signatures expected of blowout jets in coronal X-ray images. From Hinode/XRT movies and STEREO/EUVI snapshots in polar coronal holes, we present examples of (1) X-ray jets that fit the standard model, and (2) X-ray jets that do not fit the standard model but do have features appropriate for blowout jets. These features are (1) a flare arcade inside the jet-base arcade in addition to the small flare arcade (bright point) outside that standard jets have, (2) a filament of cool (T is approximately 80,000K) plasma that erupts from the core of the jetbase arcade, and (3) an extra jet strand that should not be made by the reconnection for standard jets but could be made by reconnection between the ambient unipolar open field and the opposite-polarity leg of the filament-carrying flux-rope core field of the erupting jet-base arcade. We therefore infer that these non-standard jets are blowout jets, jets made by miniature versions of the sheared-core-arcade eruptions that make CMEs

Moore, Ronald L.↗

Synthesizing Diamond from Liquid Feedstock

A relatively economical method of chemical vapor deposition (CVD) has been developed for synthesizing diamond crystals and films. Unlike prior CVD methods for synthesizing diamond, this method does not require precisely proportioned flows of compressed gas feedstocks or the use of electrical discharges to decompose the feedstocks to obtain free radicals needed for deposition chemical reactions. Instead, the feedstocks used in this method are mixtures of common organic liquids that can be prepared in advance, and decomposition of feedstock vapors is effected simply by heating. The feedstock used in this method is a solution comprising between 90 and 99 weight percent of methanol and the balance of one or more other oxyhydrocarbons that could include ethanol, isopropanol, and/or acetone. This mixture of compounds is chosen so that dissociation of molecules results in the desired proportions of carbon-containing radicals (principally, CH3) and of OH, H, and O radicals. Undesirably, the CVD temperature and pressure conditions thermodynamically favor the growth of graphite over the growth of diamond. The H radicals are desirable because they help to stabilize the growing surface of diamond by shifting the thermodynamic balance toward favoring the growth of diamond. The OH and O radicals are desirable because they preferentially etch graphite and other non-diamond carbon, thereby helping to ensure the net deposition of pure diamond. The non-methanol compounds are included in the solution because (1) methanol contains equal numbers of C and O atoms; (2) an excess of C over O is needed to obtain net deposition of diamond; and (3) the non-methanol molecules contain multiple carbon atoms for each oxygen atom and thus supply the needed excess carbon A typical apparatus used in this method includes a reservoir containing the feedstock liquid and a partially evacuated stainless-steel reaction chamber. The reservoir is connected to the chamber via tubing and a needle valve or other suitable flow controller. When the liquid enters the low-pressure environment inside the chamber, it evaporates to form a vapor mixture of the same chemical composition. In addition to the inlet for the feedstock liquid, the chamber is fitted with an outlet connected to a vacuum pump (not shown) through a throttle valve (also not shown) that is automatically controlled to keep the pressure at or near the required value throughout the deposition process. Inside the chamber, a spiral filament made of tungsten, tantalum, graphite, or other high-melting-temperature material is electrically heated to a temperature >2,000 C high enough to cause dissociation of vapor molecules into the aforementioned radicals. A deposition substrate typically, a diamond-polished silicon wafer about 2.5 cm square is positioned about 2 cm away from the filament. The exact location of the substrate is chosen so that the substrate becomes heated by the filament to a deposition temperature in the approximate range of 800 to 1,000 C.

Tzeng, Yonhua↗

Refine your search to explore more results.