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At least 181 records · Page 10
All sourcesNASA NTRSDOE OSTI
DOE OSTI2026

MassIVE MSV000100641 - Multilayered regulation by RNA thermometers enables precise control of Cas9 expression in E. coli: Cas9-based genome editing technologies can rapidly generate mutations to probe a diverse array of mutant genotypes. However, aberrant Cas9 nuclease translation and activity can occur despite the use of inducible promoters to control expression, leading to extensive cell death. This background killing caused by promoter leakiness severely limits the application of Cas9 for generating mutant libraries because of the potential for population skew. We demonstrate the utility of temperature sensitive RNA elements as a layer of post-transcriptional regulation to reduce the impact of promoter leak. We observe significant temperature-dependent increases in cell survival when certain RNA thermometers (RNATs) are placed upstream of the cas9 coding sequence. We also show that the most highly repressing RNAT, hsp17rep, significantly reduces population skew with a library of characterized guide RNAs in Escherichia coli. This strategy should be applicable to all bacterial Cas9-based methods and technologies.

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Alexander, William G. [Oak Ridge National Laborato↗
DOE OSTI2020

Integrated Circuit Design for Radiation-Hardened Charge-Sensitive Amplifier Survived up to 2 Mrad

According to the continuous development of metal-oxide semiconductor (MOS) fabrication technology, transistors have naturally become more radiation-tolerant through steadily decreasing gate-oxide thickness, increasing the tunneling probability between gate-oxide and channel. Unfortunately, despite this radiation-hardened property of developed transistors, the field of nuclear power plants (NPPs) requires even higher radiation hardness levels. Particularly, total ionizing dose (TID) of approximately 1 Mrad could be required for readout circuitry under severe accident conditions with 100 Mrad around a reactor in-core required. In harsh radiating environments such as NPPs, sensors such as micro-pocket-fission detectors (MPFD) would be a promising technology to be operated for detecting neutrons in reactor cores. For those sensors, readout circuits should be fundamentally placed close to sensing devices for minimizing signal interferences and white noise. Therefore, radiation hardening ability is necessary for the circuits under high radiation environments. This paper presents various integrated circuit designs for a radiation hardened charge-sensitive amplifier (CSA) by using SiGe 130 nm and Si 180 nm fabrication processes with different channel widths and transistor types of complementary metal-oxide-semiconductor (CMOS) and bipolar CMOS (BiCMOS). These circuits were tested under γ–ray environment with Cobalt-60 of high level activity: 490 kCi. The experiment results indicate amplitude degradation of 2.85%–34.3%, fall time increase of 201–1730 ns, as well as a signal-to-noise ratio (SNR) of 0.07–11.6 dB decrease with irradiation dose increase. These results can provide design guidelines for radiation hardening operational amplifiers in terms of transistor sizes and structures.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2023

Hydrodynamics and Survivability during Post-main-sequence Planetary Engulfment

The engulfment of substellar bodies (SBs), such as brown dwarfs and planets, by giant stars is a possible explanation for rapidly rotating giants, lithium-rich giants, and the presence of SBs in close orbits around subdwarfs and white dwarfs. We perform three-dimensional hydrodynamical simulations of the flow in the vicinity of an engulfed SB. We model the SB as a rigid body with a reflective surface because it cannot accrete. This reflective boundary changes the flow morphology to resemble that of engulfed compact objects with outflows. We measure the drag coefficients for the ram-pressure and gravitational drag forces acting on the SB, and use them to integrate its trajectory inside the star. We find that engulfment can increase the luminosity of a 1 M ⊙ star by up to a few orders of magnitude. The time for the star to return to its original luminosity is up to a few thousand years when the star has evolved to ≈10 R ⊙ and up to a few decades at the tip of the red giant branch (RGB). No SBs can eject the envelope of a 1 M ⊙ star before it evolves to ≈10 R ⊙ if the orbit of the SB is the only energy source contributing to the ejection. In contrast, SBs as small as ≈10 MJup can eject the envelope at the tip of the RGB. The numerical framework we introduce here can be used to study planetary engulfment in a simplified setting that captures the physics of the flow at the scale of the SB.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2023

Locoregional Treatment of Glioblastoma With Targeted α Therapy: [213Bi]Bi-DOTA–Substance P Versus [225Ac]Ac-DOTA–Substance P—Analysis of Influence Parameters

Background Glioblastoma (GB) is the most malignant primary brain tumor. Therefore, introduction of new treatment options is critically important. The aim of this study was to assess local treatment with α emitters [ 213 Bi]Bi-DOTA–substance P (SP) and [ 225 Ac]Ac-DOTA-SP. Methods Treatment was performed as salvage therapy in patients with recurrent primary and secondary GB. [ 213 Bi]Bi-DOTA-SP with injected activity 1.85 GBq per cycle was used in 20 primary (48.2 ± 11.8 years old) and in 9 secondary (38.8 ± 10.8 years old) GB patients and [ 225 Ac]Ac-DOTA-SP in 15 primary (45.1 ± 9.9 years old) and in 6 secondary (37.8 ± 6.4 years old) GB patients with a dose escalation scheme (10, 20, and 30 MBq). Results Local treatment with [ 213 Bi]Bi-DOTA-SP and [ 225 Ac]Ac-DOTA-SP was well tolerated with only few adverse effects. There was no statistically significant difference between [ 213 Bi]Bi-DOTA-SP and [ 225 Ac]Ac-DOTA-SP groups in survival parameters. For primary GB, survival parameters of patients treated with [ 213 Bi]Bi-DOTA-SP and [ 225 Ac]Ac-DOTA-SP were as follows(in months): progression-free survival time, 2.7 versus 2.4; OS-d (overall survival from time of diagnosis to death from any cause), 23.6 versus 21.0; OS-t (overall survival from the start of treatment to death from any cause), 7.5 versus 5.0; and OS-r (overall survival from recurrence in primary tumors to death from any cause), 10.9 versus 12.0. Survival parameters of secondary GB patients treated with [ 213 Bi]Bi-DOTA-SP and [ 225 Ac]Ac-DOTA-SP were as follows (in months): progression-free survival time, 5.8 versus 2.4; OS-d, 52.3 versus 65.0; OS-t, 16.4 versus 16.0; and OS-c (overall survival from conversion into secondary GB multiforme to death from any cause), 18.4 versus 36.0. Conclusions The similarity results of 213 Bi or 225 Ac may suggest that the local treatment of brain tumors can be greatly simplified. The experience to date shows that local radioisotope treatment of brain tumors requires further dosimetry studies, taking into account the complexity of biological processes.

Radiology, Nuclear Medicine & Medical Imaging↗
DOE OSTI2021

Data from: "Colonisation of the alpine tundra by trees: alpine neighbours assist late-seral but not early-seral conifer seedlings"

This archive contains data used to support conclusions made in “Colonisation of the alpine tundra by trees: alpine neighbours assist late-seral but not early-seral conifer seedlings”, by Jabis et al., 2020. Data were collected in the alpine field location of the Alpine Treeline Warming Experiment (ATWE), on Niwot Ridge, in the Front Range of the Colorado Rocky Mountains, USA.This package includes survivorship and physiology data for limber pine (Pinus flexilis), Engelmann spruce (Picea engelmannii), and Rocky Mountain snowlover (Chionophila jamesii). Site climate data such as soil moisture and temperature are also included. This data package contains ten comma-separated-values (.csv) files, and two rich-text-format (.rtf) files all compressed within one folder named “Neighbor_data_repository.zip”. Both file types can be opened by text-edit softwares such as TextEdit (Mac) and Notepad (Windows). The files are also compatible with analyses softwares such as R. .csv files can also be opened by Microsoft Excel. Two geospatial datasets are also included in this archive: one keyhole markup language (.kml) file with four points marking the corners of the study site, and a compressed file containing two ESRI shapefiles (.shp). The .kml files can be opened with Google Earth or Google Maps, and the shapefiles can be opened using any geographic information system applications, including the entire ArcGIS suite, and QGIS. -------------------------------------------------------------------------------------------------------------------------------------------------------The elevation mountain treeline is expected to shift upward with climate warming, and seed germination and seedling survival are critical local controls on treeline expansion. Neighboring alpine plants, either through competition for resources or through altering the microclimate, can also affect seedling emergence and survival. We asked whether establishing tree seedlings and an alpine herb are similarly sensitive to alpine plant neighbours under ambient and altered climate. We imposed active heating, watering, and neighbor removal experiments for emerging conifer seedlings and an alpine herb.We compared target plant survival, photosynthetic efficiency, and water use efficiency under ambient and experimental conditions. Picea engelmannii seedlings showed lower survival compared with Pinus flexilis three weeks following neighbour removal, and after 1 year only survived in watered plots. Pinus seedlings responded to neighbour removal by lowering the quantum yield of photosynthesis (ϕPSII). Contrary to expectations from the stress gradient hypothesis, survival was reduced without neighbours near the low-elevation range limit of Chionophila jamesii.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Development of optimal methods for collection, transportation, holding, handling, and tagging of juvenile American shad

American shad (Alosa sapidissima) are an anadromous fish species native to the United States that have an extensive range along the east coast, but their populations are declining worldwide. Acoustic telemetry can play a vital role in better understanding the behavior and survival of this sensitive species. A stepwise, systematic approach was used to assess juvenile shad survival during multiple stressors typically associated with an acoustic telemetry study. Minimizing out-of-water handling and the use of saltwater during collection increased 24-hour survival (78 to 99%) between the first two shad transports. Several experiments were conducted to determine the best transmitter design method for implanting juvenile shad. Saltwater was fundamental in keeping tagged shad alive overnight and during the tagging process. Shad as small as 50 mm were tagged using the pectoral incision method with no suture. In a 60-d holding evaluation, tagged fish survived at a comparable rate as their non-tagged counterparts (81.5% for tagged, 70% for untagged). Also, when tagged and untagged shad were exposed to a tank of predators, there was no difference in their survival. Results from this study are encouraging for improving conservation efforts for small, sensitive species of fish, like American shad. The techniques identified may help broaden the application of telemetry studies by informing researchers of optimal methods to ensure survival of tagged fish in future field studies looking to evaluate survival and entrainment rates of juvenile American shad.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Evaluation of Foster Dam Juvenile Fish Passage, 2018 (Final Report)

This report presents the results of a fish passage study conducted by the Pacific Northwest National Laboratory (PNNL) for the U.S. Army Corps of Engineers Portland District (USACE) at Foster Dam (herein, Foster) during the spring (March–June) and fall (October–December) of 2018. The goal of this study was to provide biologists, engineers, resource managers, and regional decision-makers with information about passage of juvenile Chinook salmon and juvenile steelhead (or appropriate surrogates) through specific routes at Foster. The results of this study are intended to help facilitate decision-making regarding long-term measures for enhancing salmonid passage at Foster. Radio telemetry (RT) methods were instituted at low winter reservoir forebay elevation (low pool, 613 ft mean sea level (msl); spring 2018 and fall 2018) and high summer reservoir forebay elevation (high pool, 635 ft msl; late spring and early summer 2018) to accomplish the following objectives: I. Estimate the following passage metrics under typical operations (i.e., baseline conditions): a. Overall dam-passage survival and survival specific to each route of dam passage, calculated using two different methods: i. Cormack-Jolly-Seber (CJS) estimate: Probability of survival from dam passage to detection at the Primary Array, 19 river kilometers (rkm) downstream of Foster. ii. Virtual Release/Dead Fish Correction (ViRDCt) estimate: Corrects the bias that occurs from misidentifying fish that did not survive dam-passage as alive at the tailrace Egress Array, allowing an estimate of survival from dam passage to this array, 2.5 rkm downstream of Foster. b. Route distribution (passage proportions by route; also known as “passage efficiencies”). c. Efficiency metrics i. Dam-passage efficiency (DPE), the proportion of total fish passing the dam relative to the number of total fish detected in the near forebay of the dam and therefore available to pass. ii. Fish passage efficiency (FPE), the proportion of fish passing via a non-turbine route, relative to the number of total fish in the near forebay and available to pass. iii. Fish weir efficiency (FWE) and spill bay efficiency (SBE), the proportion of fish passing the weir or spill bays, respectively, relative to the total number of fish passing the dam. d. Reservoir residency time (time elapsed between release into reservoir and dam passage) and travel times through the system. II. Estimate efficiency and effectiveness of the new fish weir, compared to the turbines and the spillway. III. Under alternative operations, compare passage through each route (turbines, weir, spillway) using a block/treatment (on/off) design similar to the 2015 and 2016 studies. IV. Estimate efficiency of the new fish weir operating at a low level of discharge (< 500 cfs) and a high level of discharge (= 500 cfs), as well as dam passage survival at each weir discharge level. V. Evaluate survival and behavior of hatchery summer steelhead to wild surrogate winter steelhead for potential use of summer steelhead as a surrogate species winter steelhead. VI. Evaluate juvenile passage rates in the auxiliary water supply (AWS), forebay water supply (FWS), and hatchery water supply (HWS).

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Population Dynamics and Temporal Trends of Bull Trout in the East Fork Salmon River, Idaho

Abstract Because of their long-term listing under the Endangered Species Act, much interest has been placed on estimating population vital rates for Bull Trout Salvelinus confluentus, but the biotic and abiotic factors that influence the interannual variability in those vital rates have rarely been evaluated. We used mark–recapture data to estimate fish growth, survival, and trends in abundance for fluvial adult Bull Trout in the East Fork Salmon River, Idaho. Over an 8-year period, a total of 1,205 individual Bull Trout were collected at a weir on the East Fork Salmon River (29 km upstream of its confluence with the Salmon River) during June–September, of which 420 were recaptures from prior years. Bull Trout varied in length from 215 to 756 mm and achieved a slightly larger asymptotic length and a slightly lower rate of growth relative to other fluvial and adfluvial Bull Trout populations. Apparent survival averaged 0.42 across all years, which was similar to previous studies estimating apparent survival for Bull Trout. The number of emigrating anadromous salmonid smolts in the upper Salmon River basin positively influenced East Fork Salmon River Bull Trout growth and survival, and survival was higher in years with lower annual discharge. Assessment of population growth via linear regression analysis indicated that the Bull Trout population was increasing during the study period (λ = 1.08; 95% confidence interval = 1.03–1.14). Our findings highlight the ecological link between the abundance of wild and hatchery Chinook Salmon Oncorhynchus tshawytscha, Sockeye Salmon O. nerka, and steelhead O. mykiss smolts and growth and survival of adult Bull Trout in systems where these species occur in sympatry.

Roth, Curtis J.↗