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All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

A Roadmap for Edge Computing Enabled Automated Multidimensional Transmission Electron Microscopy

The advent of modern, high-speed electron detectors has made the collection of multidimensional hyperspectral transmission electron microscopy datasets, such as 4D-STEM, a routine. However, many microscopists find such experiments daunting since analysis, collection, long-term storage, and networking of such datasets remain challenging. Some common issues are their large and unwieldy size that often are several gigabytes, non-standardized data analysis routines, and a lack of clarity about the computing and network resources needed to utilize the electron microscope. The existing computing and networking bottlenecks introduce significant penalties in each step of these experiments, and thus, real-time analysis-driven automated experimentation for multidimensional TEM is challenging. One solution is to integrate microscopy with edge computing, where moderately powerful computational hardware performs the preliminary analysis before handing off the heavier computation to high-performance computing (HPC) systems. In this work, we trace the roots of computation in modern electron microscopy, demonstrate deep learning experiments running on an edge system, and discuss the networking requirements for tying together microscopes, edge computers, and HPC systems.

47 OTHER INSTRUMENTATION↗
NASA NTRS2022

QWIP: A Quantitative Metric for Quality Control of Aquatic Reflectance Spectral Shape using the Apparent Visible Wavelength

The colors of the ocean and inland waters span clear blue to turbid brown, and the corresponding spectral shapes of the waterleaving signal are diverse depending on the various types and concentrations of phytoplankton, sediment, detritus and colored dissolved organic matter. Here we present a simple metric developed from a global dataset spanning blue, green and brown water types to assess the quality of a measured or derived aquatic spectrum. The Quality Water Index Polynomial (QWIP) is founded on the Apparent Visible Wavelength (AVW), a one-dimensional geophysical metric of color that is inherently correlated to spectral shape calculated as a weighted harmonic mean across visible wavelengths. The QWIP represents a polynomial relationship between the hyperspectral AVW and a Normalized Difference Index (NDI) using red and green wavelengths. The QWIP score represents the difference between a spectrum’s AVW and NDI and the QWIP polynomial. The approach is tested extensively with both raw and quality controlled field data to identify spectra that fall outside the general trends observed in aquatic optics. For example, QWIP scores less than or greater than 0.2 would fail an initial screening and be subject to additional quality control. Common outliers tend to have spectral features related to: 1) incorrect removal of surface reflected skylight or 2) optically shallow water. The approach was applied to hyperspectral imagery from the Hyperspectral Imager for the Coastal Ocean (HICO), as well as to multispectral imagery from the Visual Infrared Imaging Radiometer Suite (VIIRS) using sensor-specific extrapolations to approximate AVW. This simple approach can be rapidly implemented in ocean color processing chains to provide a level of uncertainty about a measured or retrieved spectrum and flag questionable or unusual spectra for further analysis.

remote sensing reflectance↗
DOE OSTI2025

High-throughput micro-scale bandgap mapping for perovskite-inspired materials with complex composition space

Abstract To realize the full promise of high-throughput experimental workflows, the rate of sample synthesis must be matched by that of characterization. Of growing interest are contactless optical techniques that can rapidly measure material homogeneity and properties. Here, we present a hyperspectral imaging method to measure local optical bandgap distributions within samples, utilizing spatially-resolved reflectance spectra coupled with automated data analysis. We collect approximately one million optical bandgap data across the compositional space of Cs 3 (Bi x Sb 1-x ) 2 (Br y I 1-y ) 9 perovskite-inspired materials. Our results show non-monotonic bandgap variations (i.e., bandgap bowing) along six composition gradient sequences, in addition to identifying samples with multiple bandgaps in statistics. High-throughput transient absorption spectroscopy reveals that within these compositions, the depletion of the ground state carriers to excited states occurred at discrete energy levels with independent carrier dynamics, consistent with the bandgap observation and indicative of phase separation. This work demonstrates the potential for rapid optical measurements to assess material quality and homogeneity in a high-throughput experimental setting, supporting screening and recipe optimization of optoelectronic material candidates with desired carrier dynamics and optical properties.

Science & Technology - Other Topics↗
DOE OSTI2025

SELKIE Spectral Extraction Results

This document describes results from the WAMOR Chesapeake Bay collection in October 2024, a data collection from the Arkeus HSOR hyperspectral sensor. The calibration array was staged at USCG Station Little Creek, Virginia. With the provided HSOR imagery and data, our analysis does not show a capability to effectively detect materials of interest from non-materials of interest as well as differentiate materials of interest from each other, the materials of interest being polypropylene, polyester, and cotton. The fundamental reasons are (1) the spectral bands of HSOR do not reside in specific wavelength locations to allow for material identification and (2) the provided imagery appears to not have consistent band-to-band relationships indicative of a lack of calibration (gain/offsets) or some other calibration issues. With regard to analyzing a subset of the delivered HSOR, HSOR can make general statements about materials not being water and have the capability to make color assessments.

36 MATERIALS SCIENCE↗
NASA NTRS1998

Diurnal Reflectance Changes in Vegetation Observed with AVIRIS

Among the most important short-term dynamic biological processes are diurnal changes in canopy water relations. Plant regulation of water transport through stomatal openings affects other gaseous transport processes, often dramatically decreasing photosynthetic fixation of carbon dioxide during periods of water stress. Water stress reduces stomatal conductance of water vapor through the leaf surface and alters the diurnal timing of stomatal opening. Under non-water stressed conditions, stomates typically open soon after dawn and transpire water vapor throughout the daylight period. During stress periods, stomates may close for part of the day, generally near mid-day. Under prolonged stress conditions, stomatal closure shifts to earlier times during the day; stomates may close by mid-morning and remain closed until the following morning - or remain closed entirely. Under these conditions the relationship between canopy greenness (e.g., measured with a vegetation index or by spectral mixture analysis) and photosynthetic fixation of carbon is lost and the remotely sensed vegetation metric is a poor predictor of gas exchange. Prediction of stomatal regulation and exchange of water and trace gases is critical for ecosystem and climate models to correctly estimate budgets of these gases and understand or predict other processes like gross and net ecosystem primary production. Plant gas exchange has been extensively studied by physiologists at the leaf and whole plant level and by biometeorologists at somewhat larger scales. While these energy driven processes follow a predictable if somewhat asymmetric diurnal cycle dependent on soil water availability and the constraints imposed by the solar energy budget, they are nonetheless difficult to measure at the tree and stand levels using conventional methods. Ecologists have long been interested in the potential of remote sensing for monitoring physiological changes using multi-temporal images. Much of this research has focused on day-to-day changes in water use, especially for agricultural applications. Ustin et al. showed seasonal changes in canopy water content in chaparral shrub could be estimated using optical methods. Vanderbilt et al. followed asymmetric diurnal changes in the reflectance of a walnut orchard, but could not attribute specific reflectance changes to specific changes in canopy architecture or physiology. Forests and shrub lands in California experience prolonged periods of drought, sometimes extending six months without precipitation. The conifer and evergreen chaparral communities common to the foothill region around the central valley of California retain their foliage throughout the summer and have low transpiration rates despite high net radiation and temperature conditions. In contrast, grasslands and drought resistant deciduous species in the same habitat are seasonally dormant in summer. Because of differences in the mechanisms of drought tolerance, rooting depth and physiology between different plant communities in the region, it is likely that they display differences in diurnal water relations. The presence of diverse plant communities provides an opportunity to investigate possible diurnal landscape patterns in water relations that could be observed by an airborne hyperspectral scanner. This investigation of AVIRIS data collected over forest and shrub land represents the continuation of a prior investigation involving spectral mixture analysis of diurnal effects in the same AVIRIS data set.

Vanderbilt, V. C.↗
NASA NTRS2015

A Module for Assimilating Hyperspectral Infrared Retrieved Profiles into the Gridpoint Statistical Interpolation System for Unique Forecasting Applications

Hyperspectral infrared sounder radiance data are assimilated into operational modeling systems however the process is computationally expensive and only approximately 1% of available data are assimilated due to data thinning as well as the fact that radiances are restricted to cloud-free fields of view. In contrast, the number of hyperspectral infrared profiles assimilated is much higher since the retrieved profiles can be assimilated in some partly cloudy scenes due to profile coupling other data, such as microwave or neural networks, as first guesses to the retrieval process. As the operational data assimilation community attempts to assimilate cloud-affected radiances, it is possible that the use of retrieved profiles might offer an alternative methodology that is less complex and more computationally efficient to solve this problem. The NASA Short-term Prediction Research and Transition (SPoRT) Center has assimilated hyperspectral infrared retrieved profiles into Weather Research and Forecasting Model (WRF) simulations using the Gridpoint Statistical Interpolation (GSI) System. Early research at SPoRT demonstrated improved initial conditions when assimilating Atmospheric Infrared Sounder (AIRS) thermodynamic profiles into WRF (using WRF-Var and assigning more appropriate error weighting to the profiles) to improve regional analysis and heavy precipitation forecasts. Successful early work has led to more recent research utilizing WRF and GSI for applications including the assimilation of AIRS profiles to improve WRF forecasts of atmospheric rivers and assimilation of AIRS, Cross-track Infrared and Microwave Sounding Suite (CrIMSS), and Infrared Atmospheric Sounding Interferometer (IASI) profiles to improve model representation of tropopause folds and associated non-convective wind events. Although more hyperspectral infrared retrieved profiles can be assimilated into model forecasts, one disadvantage is the retrieved profiles have traditionally been assigned the same error values as the rawinsonde observations when assimilated with GSI. Typically, satellitederived profile errors are larger and more difficult to quantify than traditional rawinsonde observations (especially in the boundary layer), so it is important to appropriately assign observation errors within GSI to eliminate potential spurious innovations and analysis increments that can sometimes arise when using retrieved profiles. The goal of this study is to describe modifications to the GSI source code to more appropriately assimilate hyperspectral infrared retrieved profiles and outline preliminary results that show the differences between a model simulation that assimilated the profiles as rawinsonde observations and one that assimilated the profiles in a module with the appropriate error values.

Berndt, Emily↗
NASA NTRSDate not supplied

Tools for Performing SBG hyperspectral Observing System Simulation Experiment

One of NASA’s Decadal Survey mission, Surface Biology and Geology (SBG), will include a hyperspectral remote sensing imager, which has a very high spatial resolution and a wide spectral coverage (from UV to Near IR). Unprecedented large data volumes will be generated by the SBG hyperspectral instrument. Before the launch of the new satellite, an Observing System Simulation Experiment (OSSE) can be used to study different designs of the new satellite system. One of the key components in an OSSE study is a radiative transfer model (RTM) or forward model. In this presentation, we will describe a Principal Component-based Radiative Transfer Model (PCRTM) which is capable of simulating atmospheric (TOA) radiance or reflectance spectra from far IR to visible and UV spectral regions (50 wavenumber to 30000 wavenumber) quickly and accurately. Multiple scattering from multiple layers of clouds/aerosols are included in the model. The PCRTM has a very good accuracy relative to reference line-by-line radiative transfer models (LBLRTM), and it saves 3-4 orders of magnitude computational time relative to LBLRTM or MODTRAN. The PCRTM model has been successfully used to analyze large volumes of data from hyperspectral sensors such as AIRS, CrIS, and IASI. It has also been used to perform OSSE studies for the Climate Absolute Radiance and Refractivity Observatory (CLARREO) mission. Another useful tool for the OSSE is surface Bidirectional Reflectance Distribution Function (BRDF) database. It is very crucial for the SBG OSSE to include realistic BRDF spectra. Currently, most of the surface reflectance spectra such as those in the ECOSIS and ECOSTRESS are measured at specific observation geometries. We have developed a hyperspectral bidirectional reflectance (HSBR) model which combines Ross-Li BRDF model with the existing reflectance spectral libraries using a principal component analysis. This HSBR model can provide realistic BRDF spectra under various observation conditions. It can also be used to generate realistic BRDF spectra using measurements from multi-band imagers or spectrometers such as MODIS or VIIRS.

Xu Liu↗
NASA NTRS2022

Verification and Validation of Hybridspectral Radiometry Obtained from an Unmanned Surface Vessel (USV) in the Open and Coastal Oceans

The hardware and software capabilities of the compact-profiling hybrid instrumentation for radiometry and ecology (C-PHIRE) instruments on an unmanned surface vessel (USV) are evaluated. Both the radiometers and USV are commercial-off-the-shelf (COTS) products, with the latter being only minimally modified to deploy the C-PHIRE instruments. The hybridspectral C-PHIRE instruments consist of an array of 18 multispectral microradiometers with 10 nm wavebands spanning 320–875 nm plus a hyperspectral compact grating spectrometer (CGS) with 2048 pixels spanning 190–1000 nm. The C-PHIRE data were acquired and processed using two architecturally linked software packages, thereby allowing lessons learned in one to be applied to the other. Using standard data products and unbiased statistics, the C-PHIRE data were validated with those from the well-established compact-optical profiling system (C-OPS) and verified with the marine optical buoy (MOBY). Agreement between algorithm variables used to estimate colored dissolved organic matter (CDOM) absorption and chlorophyll a concentration were also validated. Developing and operating novel technologies, such as the C-PHIRE series of instruments, deployed on a USV increase the frequency and coverage of optical observations, which are required to fully support the present and next-generation validation exercises in radiometric remote sensing of aquatic ecosystems.

Hybridspectral↗
NASA NTRSDate not supplied

Status and Progress of Observation Usages in the GMAO GEOS Atmospheric Data Assimilation System

The Global Modeling and Assimilation Office (GMAO) continuously works to enhance the use of observations in the Goddard Earth Observing System – Forward Processing (GEOS-FP) model and analysis system. Updates to GEOS-FP in March 2022 and February 2023 focused on the analysis component, adding new capabilities to ingest radiances and other new observing systems, such as SPIRE GNSS-RO data. Concurrent activities enhance aspects of the radiance assimilation, such as the use of hyperspectral radiances in the stratosphere and lower troposphere, the introduction of microwave radiances over land, and a revised treatment of GMI observations. Furthermore, GMAO has been contributing to the Joint Effort for Data assimilation Integration (JEDI), including development and testing of various components of the observing system and GEOS-specific background error covariances that will allow migration of GEOS-FP from a GSI- to a JEDI-based analysis system. A number of examples are shown.

Y Zhu↗
NASA NTRS2011

Demonstrating the Operational Value of Atmospheric Infrared Sounder (AIRS) Retrieved Profiles in the Pre-Convective Environment

The Short-term Prediction Research and Transition Center (SPoRT) is a collaborative partnership between NASA and operational forecasting partners, including a number of National Weather Service offices. SPoRT provides real-time NASA products and capabilities to its partners to address specific operational forecast challenges. One operational forecast challenge is forecasting convective weather in data-void regions such as large bodies of water (e.g. Gulf of Mexico). To address this forecast challenge, SPoRT produces a twice-daily three-dimensional analysis that blends a model first-guess from the Advanced Research Weather Research and Forecasting (WRF-ARW) model with retrieved profiles from the Atmospheric Infrared Sounder (AIRS) -- a hyperspectral sounding instrument aboard NASA's Aqua satellite that provides temperature and moisture profiles of the atmosphere. AIRS profiles are unique in that they give a three dimensional view of the atmosphere that is not available through the current rawinsonde network. AIRS has two overpass swaths across North America each day, one valid in the 0700-0900 UTC timeframe and the other in the 1900-2100 UTC timeframe. This is helpful because the rawinsonde network only has data from 0000 UTC and 1200 UTC at specific land-based locations. Comparing the AIRS analysis product with control analyses that include no AIRS data demonstrates the value of the retrieved profiles to situational awareness for the pre-convective (and convective) environment. In an attempt to verify that the AIRS analysis was a good representation of the vertical structure of the atmosphere, both the AIRS and control analyses are compared to a Rapid Update Cycle (RUC) analysis used by operational forecasters. Using guidance from operational forecasters, convective available potential energy (CAPE) was determined to be a vital variable in making convective forecasts and is used herein to demonstrate the utility of the AIRS profiles in changing the vertical thermodynamic structure of the atmosphere in the pre-convective and convective environment. CAPE is an important metric because of it is a quantitative measure of atmospheric stability, which is necessary information when forecasting for convective weather. Case studies from the summer of 2010 were examined, and most impact from the AIRS retrieved profiles occurred over the data-void Gulf of Mexico with fields of convective potential closer to the RUC than the CNTL. Mixed results were found when AIRS retrieved profiles were used over land, so more cases need to be examined to determine whether AIRS would be an effective tool over land. Additional analyses of problematic convective forecasts over the Gulf Coast will be needed to determine the operational impact of AIRS. SPoRT eventually plans to transition the AIRS product to select Weather Forecast Office (WFO) partners, pending the outcome of these additional analyses.

Kozlowski, Danielle M.↗
DOE OSTI2022

Simulation-guided analysis of resonant soft X-ray scattering for determining the microstructure of triblock copolymers

Resonant soft X-ray scattering (RSoXS) probes structure with chemical sensitivity that is useful for determining the morphology of multiblock copolymers. However, the hyperspectral scattering data produced by this technique can be challenging to interpret. Here, in this study, we use computational scattering simulations to extract the microstructure of a model triblock copolymer from the energy-dependent scattering from RSoXS. An ABC triblock terpolymer formed from poly(4-methylcaprolactone) (P4MCL), poly(2,2,2-trifluoroethylacrylate) (PTFEA), and poly (dodecylacrylate) (PDDA), P4MCL-block-PTFEA-block-PDDA, was synthesized as the model triblock system. Through quantitative evaluation of simulated scattering data from a physics-informed set of candidate structure models against experimental RSoXS data, we find the best agreement with hexagonally packed core–shell cylinders. This result is also consistent with electron-density reconstruction from hard X-ray scattering data evaluated against electron-density maps generated with the same model set. These results demonstrate the utility of simulation-guided scattering analysis to study complex microstructures that are challenging to image by microscopy.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS2009

Recent Advances in Techniques for Hyperspectral Image Processing

Imaging spectroscopy, also known as hyperspectral imaging, has been transformed in less than 30 years from being a sparse research tool into a commodity product available to a broad user community. Currently, there is a need for standardized data processing techniques able to take into account the special properties of hyperspectral data. In this paper, we provide a seminal view on recent advances in techniques for hyperspectral image processing. Our main focus is on the design of techniques able to deal with the highdimensional nature of the data, and to integrate the spatial and spectral information. Performance of the discussed techniques is evaluated in different analysis scenarios. To satisfy time-critical constraints in specific applications, we also develop efficient parallel implementations of some of the discussed algorithms. Combined, these parts provide an excellent snapshot of the state-of-the-art in those areas, and offer a thoughtful perspective on future potentials and emerging challenges in the design of robust hyperspectral imaging algorithms

Plaza, Antonio↗
NASA NTRS2017

Climate Data Guide - Modern Era Retrospective Analysis for Research and Applications, version 2 (MERRA-2)

The Modern-Era Retrospective analysis for Research and Applications, version 2 (MERRA-2) is a global atmospheric reanalysis produced by the NASA Global Modeling and Assimilation Office (GMAO). It spans the satellite observing era from 1980 to the present. The goals of MERRA-2 are to provide a regularly-gridded, homogeneous record of the global atmosphere, and to incorporate additional aspects of the climate system including trace gas constituents (stratospheric ozone), and improved land surface representation, and cryospheric processes. MERRA-2 is also the first satellite-era global reanalysis to assimilate space-based observations of aerosols and represent their interactions with other physical processes in the climate system. The inclusion of these additional components are consistent with the overall objectives of an Integrated Earth System Analysis (IESA). MERRA-2 is intended to replace the original MERRA product, and reflects recent advances in atmospheric modeling and data assimilation. Modern hyperspectral radiance and microwave observations, along with GPS-Radio Occultation and NASA ozone datasets are now assimilated in MERRA-2. Much of the structure of the data files remains the same in MERRA-2. While the original MERRA data format was HDF-EOS, the MERRA-2 supplied binary data format is now NetCDF4 (with lossy compression to save space).

MERRA-2↗
NASA NTRS2023

Calibration of the SNPP and NOAA 20 VIIRS Sensors for Continuity of the MODIS Climate Data Records

Accurate long-term sensor calibration and periodic re-processing to ensure consistency and continuity of atmospheric, land and ocean geophysical retrievals from space within the mission period and across different missions is a major requirement of climate data records. In this work, we applied the Multi-Angle Implementation of Atmospheric Correction (MAIAC)-based vicarious calibration technique over Libya-4 desert site to perform calibration analysis of Visible Infrared Imaging Radiometer Suite (VIIRS) on Suomi National Polar-orbiting Partnership (SNPP) and NOAA-20 satellites. For both VIIRS sensors we characterized residual linear calibration trends and cross-calibrated both sensors to MODerate resolution Imaging Spectroradiometer (MODIS) Aqua regarded as a calibration standard. The relative spectral response (RSR) differences were accounted for using the German Aerospace Center (DLR) Earth Sensing Imaging Spectrometer (DESIS) hyperspectral surface reflectance data. Our results agree with independent vicarious calibration results of both the MODIS/VIIRS Characterization Support Team as well as the CERES Imager and Geostationary Calibration Group within estimated uncertainty of 1–2%. Analysis of MAIAC geophysical products with the new calibration shows a high level of agreement of MAIAC aerosol, surface reflectance and NDVI records between MODIS and VIIRS. Excluding high aerosol optical depth (AOD), all three sensors agree in AOD with mean difference (MD) less than 0.01 and residual mean squared difference rmsd ∼ 0.04. Spectral geometrically normalized surface reflectance agrees within rmsd of 0.003–0.005 in the visible and 0.01–0.012 at longer wavelengths. The residual surface reflectance differences are fully explained by differences in spectral filter functions. Finally, difference in NDVI is characterized by rmsd ∼ 0.02 and MD less than 0.003 for NDVI based on VIIRS imagery bands I1/I2 and less than 0.01 for NDVI based on VIIRS radiometric bands M5/M7. In practical sense, these numbers indicate consistency and continuity in MAIAC records ensuring the smooth transition from MODIS to VIIRS.

MAIAC↗
NASA NTRS2012

Improved Impact of Atmospheric Infrared Sounder (AIRS) Radiance Assimilation in Numerical Weather Prediction

Improvements to global and regional numerical weather prediction (NWP) have been demonstrated through assimilation of data from NASA s Atmospheric Infrared Sounder (AIRS). Current operational data assimilation systems use AIRS radiances, but impact on regional forecasts has been much smaller than for global forecasts. Retrieved profiles from AIRS contain much of the information that is contained in the radiances and may be able to reveal reasons for this reduced impact. Assimilating AIRS retrieved profiles in an identical analysis configuration to the radiances, tracking the quantity and quality of the assimilated data in each technique, and examining analysis increments and forecast impact from each data type can yield clues as to the reasons for the reduced impact. By doing this with regional scale models individual synoptic features (and the impact of AIRS on these features) can be more easily tracked. This project examines the assimilation of hyperspectral sounder data used in operational numerical weather prediction by comparing operational techniques used for AIRS radiances and research techniques used for AIRS retrieved profiles. Parallel versions of a configuration of the Weather Research and Forecasting (WRF) model with Gridpoint Statistical Interpolation (GSI) that mimics the analysis methodology, domain, and observational datasets for the regional North American Mesoscale (NAM) model run at the National Centers for Environmental Prediction (NCEP)/Environmental Modeling Center (EMC) are run to examine the impact of each type of AIRS data set. The first configuration will assimilate the AIRS radiance data along with other conventional and satellite data using techniques implemented within the operational system; the second configuration will assimilate AIRS retrieved profiles instead of AIRS radiances in the same manner. Preliminary results of this study will be presented and focus on the analysis impact of the radiances and profiles for selected cases.

Zavodsky, Bradley↗
DOE OSTI2025

Spatially Resolved Raman Spectroscopy of Thin Carbon Interphase in SiC Ceramic Matrix Composites

A dedicated analysis method is presented to extract the Raman spectrum of an interphase layer thinner than the laser spot size. We focused on spatial correlations between the contrast of optical micrographs and Raman hyperspectral data to predict the constituents of the mixed spectra measured near the interphase. By employing a mapping step size of 0.1 μm, the Raman spectrum of approximately 0.3-μm-thick carbon interphase in a SiC fiber-reinforced SiC matrix composite was extracted from data acquired with a theoretical spot size of about 0.7 μm. Notably, conventional chemometrics procedures were unable to isolate the interphase signal, instead producing a spectrum representing a mixture of interphase and matrix. This study used another composite with approximately 0.9-μm-thick interphase to validate the analysis method, enabling direct measurement of the interphase spectrum. The proposed Raman analysis method has advantages in specimen volume and turnaround time compared to traditional characterization methods, such as transmission electron microscopy. In conclusion, this study also evaluates the applicability of the analysis method to different composite materials and identifies key requirements of the measurements, including the ratio of interphase thickness to spot size and the homogeneity of the surrounding matrix.

ceramic matrix composite↗
DOE OSTI2023

Longitudinal Single‐Cell Imaging of Engineered Strains with Stimulated Raman Scattering to Characterize Heterogeneity in Fatty Acid Production

Abstract Understanding metabolic heterogeneity is critical for optimizing microbial production of valuable chemicals, but requires tools that can quantify metabolites at the single‐cell level over time. Here, longitudinal hyperspectral stimulated Raman scattering (SRS) chemical imaging is developed to directly visualize free fatty acids in engineered Escherichia coli over many cell cycles. Compositional analysis is also developed to estimate the chain length and unsaturation of the fatty acids in living cells. This method reveals substantial heterogeneity in fatty acid production among and within colonies that emerges over the course of many generations. Interestingly, the strains display distinct types of production heterogeneity in an enzyme‐dependent manner. By pairing time‐lapse and SRS imaging, the relationship between growth and production at the single‐cell level are examined. The results demonstrate that cell‐to‐cell production heterogeneity is pervasive and provides a means to link single‐cell and population‐level production.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRSDate not supplied

PyroCbs from Australia Fires and its Impact Using Satellite Observations from CrIS and TROPOMI and Reanalysis Data

Pyrocumulonimbus (pyroCb) clouds are thunder clouds created by intense heat from the Earth’s surface. They are formed similarly to cumulonimbus clouds, but the intense heat that results in the vigorous updraft comes from fire, either large wildfires or volcanic eruptions. Australia’s unprecedented fire disasters at the end of 2019 to early 2020 emitted huge amounts of carbon monoxide (CO) and fire aerosol particles to the atmosphere, particularly during the pyroCb outbreak that occurred in southeast Australia between 29 December 2019 and 4 January 2020. It was estimated that at least 18 pyroCbs were generated during this episode, and some of them injected ice, smoke, and biomass burning gases above the local tropopause. An unprecedented abundance of H2O and CO in the stratosphere, and the displacement of background ozone (O3) and N2O from rapid ascent of air from the troposphere and lower stratosphere were found from satellite observations. Some other studies also found that the fire emissions and their long-range transport resulted in stratospheric aerosol, temperature, and O3 anomalies after the 2020 Australian bushfires and altered the Antarctic ozone and vortex, posing great impact to local air quality and climate change. Further study on the atmospheric thermodynamic status of atmosphere associated with these pyroCbs, and the change of the cloud properties and trace gases during this unprecedented Australia fires will be made using a new single Field of View (SFOV) Sounder Atmospheric Products (SiFSAP). SiFSAP was developed by NASA using the Cross-track Infrared Sounder (CrIS) and Advanced Technology Microwave Sounder (ATMS) onboard SNPP and JPSS-1, and will soon be available to the public at NASA DAAC. Since this product has a spatial resolution of 15 km at nadir, which is better than most global weather and climate models and other current operational sounding products, a process-oriented analysis of the dynamic transport of CO and fire plumes during this unprecedented fire disasters will be made in this study. Based on a Principal Component Radiative Transfer Model (PCRTM) and an optimized estimation retrieval algorithm, a simultaneously retrieval is made using the whole spectral information measured by CrIS, and the derived SiFSAP include temperature, water vapor, trace gases (such as O3, CO2, CO, CH4 and N2O), cloud properties and surface properties. Use of ATMS together with CrIS allows SiFSAP to get accurate retrieval products under thick pyroCb conditions, and an algorithm to detect pyroCb based on the hyperspectral infrared sounder spectrum from CrIS will be developed and verified. In addition to SiFSAP sounding products, other products like CO, O3, NO2 from TROPOMI, O3 from OMPS will be used for retrospective analysis. The wind fields from the NASA’s Modern-Era Retrospective Analysis for Research and Applications Version-2 (MERRA-2) and ERA5 will be used to characterize the transport, and the SiFSAP temperature and water vapor profiles within and around pyroCbs will be compared with MERRA-2 and ERA5 products.

Xiaozhen (Shawn) Xiong↗