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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 181 records · Page 10
All sourcesNASA NTRSDOE OSTI
NASA NTRS2017

Field Exploration and Life Detection Sampling Through Planetary Analogue Sampling (FELDSPAR).

Exploration missions to Mars rely on rovers to perform analyses over small sampling areas; however, landing sites for these missions are selected based on large-scale, low-resolution remote data. The use of Earth analogue environments to estimate the multi-scale spatial distributions of key signatures of habitability can help ensure mission science goals are met. A main goal of FELDSPAR is to conduct field operations analogous to Mars sample return in its science, operations, and technology from landing site selection, to in-field sampling location selection, remote or stand-off analysis, in situ analysis, and home laboratory analysis. Lava fields and volcanic regions are relevant analogues to Martian landscapes due to desiccation, low nutrient availability, and temperature extremes. Operationally, many Icelandic lava fields are remote enough to require that field expeditions address several sampling constraints that are experienced in robotic exploration, including in situ and sample return missions. The Fimmvruhls lava field was formed by a basaltic effusive eruption associated with the 2010 Eyjafjallajkull eruption. Mlifellssandur is a recently deglaciated plain to the north of the Myrdalsjkull glacier. Holuhraun was formed by a 2014 fissure eruptions just north of the large Vatnajkull glacier. Dyngjusandur is an alluvial plain apparently kept barren by repeated mechanical weathering. Informed by our 2013 expedition, we collected samples in nested triangular grids every decade from the 10 cm scale to the 1 km scale (as permitted by the size of the site). Satellite imagery is available for older sites, and for Mlifellssandur, Holuhraun, and Dyngjusandur we obtained overhead imagery at 1 m to 200 m elevation. PanCam-style photographs were taken in the field by sampling personnel. In-field reflectance spectroscopy was also obtained with an ASD spectrometer in Dyngjusandur. All sites chosen were 'homogeneous' in apparent color, morphology, moisture, grain size, and reflectance spectra at all scales greater than 10 cm. Field lab assays were conducted to monitor microbial habitation, including ATP quantification, qPCR for fungal, bacterial, and archaeal DNA, and direct cell imaging using fluorescence microscopy. Home laboratory analyses include Raman spectroscopy and community sequencing. ATP appeared to be significantly more sensitive to small changes in sampling location than qPCR or fluorescence microscopy. Bacterial and archaeal DNA content were more consistent at the smaller scales, but similarly variable across more distant sites. Conversely, cell counts and fungal DNA content have significant local variation but appear relatively homogeneous over scales of 1 km. ATP, bacterial DNA, and archaeal DNA content were relatively well correlated at many spatial scales. While we have observed spatial variation at various scales and are beginning to observe how that variation fluctuates over time as biodiversity recovers after an eruption, we do not yet fully understand what parameters lead to the observed spatial variation. Home laboratory analyses will help us further understand the elemental and structural composition of the basaltic matrices, but further field analyses are vital for the understanding how temperature, moisture, incident radiation, and so forth influence the habitability of a microclimate.

Field↗
NASA NTRS2017

High-Spatial-Resolution OH and CH2O PLIF Visualization in a Dual-Mode Scramjet Combustor

A high-spatial-resolution planar laser-induced fluorescence (PLIF) imaging system was constructed and used to image a cavity-stabilized, premixed ethylene-air flame. The flame was created within a continuous flow, electrically-heated supersonic combustion facility consisting of a Mach 2 nozzle, an isolator with flush-wall fuel injectors, a combustor with a cavity flameholder of height 9 mm and optical access, and an extender. Tests were conducted at total temperature 1200 K, total pressure 300 kPa, equivalence ratio near 0.4 in the combustor, and Mach number near 0.6 in the combustor. A frequency-doubled Nd:YAG laser pumped a dye laser, which produced light at 283.55 nm. The beam was shaped into a light sheet with full width half-maximum 25 microns, which illuminated a streamwise plane that bisected the cavity. An intensified camera system imaged OH in this plane with a square 6.67 mm field of view and in-plane resolution 39 microns. Images were taken between the backward-facing step and 120 mm downstream of the step. OH structures as small as 110 microns were observed. CH2O was excited using 352.48 nm light; the smallest observed CH2O structures were approximately 200 microns wide. Approximately 15,000 images per species were processed and used to compute composite images.

Geipel, Clayton M.↗
NASA NTRS2018

Curating NASA's Past, Present, and Future Extraterrestrial Sample Collections

As codified in NASA Policy Directive 7100.10F, the Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (hereafter JSC Curation) is charged with curation of all extraterrestrial material under NASA control, including future NASA missions. JSC Curation curates all or part of nine astromaterial collections in seven clean room suites: (1) Apollo Samples (1969; ISO 6-7), (2) Luna Samples (from USSR; 1972; ISO 7), (3) Antarctic Meteorites (1976; ISO 7), (4) Cosmic Dust (1981; ISO 5), (5) Microparticle Impact Collection (formerly called Space Exposed Hardware; 1985; ISO 5), (6) Genesis Solar Wind Atoms (2004; ISO 4); (7) Stardust Comet Particles (2006; ISO 5), (8) Stardust Interstellar Particles (2006; ISO 5), (9) Hayabusa Asteroid Particles (from JAXA; 2010; ISO 5). In addition to the labs that house the samples, we have installed and maintained a wide variety of facilities and infrastructure required to support the clean-rooms: more than 10 different HEPA-filtered air-handling systems, ultrapure dry gaseous nitrogen systems, an ultrapure water system (UPW) and cleaning facilities to provide clean tools and equipment for the labs. We also have sample preparation facilities for making thin sections, microtome sections, and even focused ion-beam (FIB) sections to meet the research requirements of scientists. To ensure that we are keeping the samples as pristine as possible, we routinely monitor the cleanliness of our clean rooms and infrastructure systems. This monitoring includes: daily monitoring of the quality of our UPW, weekly airborne particle counts in the labs, monthly monitoring of the stable isotope composition of the gaseous N2 system, and annual measurements of inorganic or organic contamination in processing cabinets. We track within our databases the current and ever-changing characteristics of more than 250,000 individual samples across our various collections (including the 19,141 samples on loan to 433 Principal Investigators in 24 countries). The next sample return missions that NASA will participate in are Hayabusa2 and OSIRIS-REx (Origins Spectral Interpretation Resource Identification Security - Regolith Explorer). The designs for a new state-of-the-art suite of clean rooms to house these samples at JSC have been finalized. This includes separate ISO class 5 clean rooms to house each collection, a common ISO class 7 area for general use, an ISO class 7 microtome laboratory, and a separate thin section lab. Additionally, a new cleaning facility is being designed and procedures developed that will allow for enhanced cleaning of cabinets and tools in an inorganically, organically, and biologically clean manner. We are also designing a large multi-purpose Advanced Curation laboratory that will allow us to develop the techniques necessary to fully support the Hayabusa2 and OSIRIS-REx missions, as well as future possible sample return missions (e.g., Lunar Polar Volatiles, Mars, Comet Surface). A micro-CT (micro Computed Tomography) laboratory dedicated to the study of astromaterials has come online within JSC Curation, and we plan to add additional facilities that will enable non-destructive (or minimally-destructive) analyses of astromaterials in the near future (e.g., micro-XRF (micro X-Ray Fluorescence), confocal imaging Raman Spectroscopy). These facilities will be available to: (1) develop sample handling and storage techniques for future sample return missions, (2) be utilized by PET (Positron Emission Tomography) for future sample return missions, (3) for retroactive PET-style analyses of our existing collections, and (4) for periodic assessments of the existing sample collections.

Zeigler, Ryan A.↗
NASA NTRS2019

The Effect of Turbulence Modeling on the Mixing Characteristics of Several Fuel Injectors at Hypervelocity Flow Conditions

CFD analysis is presented on the effects of turbulence modeling choices on the mixing characteristics and performance of three fuel injectors at hypervelocity flow conditions. The analyses were carried out with the VULCAN-CFD solver using Reynolds-Averaged Simulations (RAS). The hypervelocity flow conditions match the high Mach number flow of the experiments conducted as a part of the Enhanced Injection and Mixing Project (EIMP) at the NASA Langley Research Center. The three injectors are the baseline configurations used in the experiments and represent three categories of injectors typically considered individually or in combination for fueling high-speed propulsive devices. The current work discusses the impact of the turbulence model and the turbulent Schmidt number on the mixing flow field behavior and the mixing performance as described by the one-dimensional values of the Mach number, total pressure recovery, and the mixing efficiency. Because planar laser induced fluorescence (PLIF) images are available from the EIMP experiments, the sensitivity of the synthetic LIF signal to turbulence modeling choices is also examined to determine whether PLIF can be extended beyond its intended qualitative visualization purpose and used to guide CFD turbulence model and parameter selections. It is found that the mixing performance, as quantified using mixing efficiency, exhibits a strong sensitivity to both turbulence model choice and turbulent Schmidt number value. However, the synthetic LIF signal only demonstrates a modest level of sensitivity, which suggests that PLIF is of limited use for guiding CFD turbulence model and parameter selections.

Drozda, Tomasz G.↗
NASA NTRS2021

SMD Technology Highlights

Six technology highlights from the Science Mission Directorate (SMD) Heliophysics, Astrophysics, Earth, and Biological and Physical Sciences divisions. Technologies featured include 1. the 1653 m2 Solar Cruiser Solar Sail Propulsion system to enable missions to reach novel and otherwise difficult or impossible destinations for observing the Sun. 2. Technology sponsored by NASA and developed by MIT Space Nanotechnology Lab is developing high-performance space instrumentation for more powerful future X-ray telescopes that will study the dynamics of the high-energy universe. 3. Active thermal control technology that will help enable large ultra-stable telescopes needed to detect and characterize Earth-like planets around other stars. 4. SMD sponsored high-performance infrared sensors with reduced requirements for cooling onboard satellites and these sensors could potentially be flown on small satellite platforms like CubeSats. 5. A new technology that will utilize electron beams/ultraviolet light to remove dust that could help protect future space assets and astronauts from dangerous effects that arise from Moon/Mars dust exposure. 6. Studies using model organisms on ISS to understand the impact of the spaceflight environment on organisms in preparation for long-duration missions.

solar sail↗
NASA NTRS2022

Simultaneous 100-kHz Acetone Planar Laser-Induced Fluorescence and OH* Chemiluminescence in a Linear Non-Premixed Detonation Channel

Reactant mixing and combustion are investigated in an optically accessible, self-excited linear detonation combustor. The mixing field is captured using 100 kHz planar laser-induced fluorescence (PLIF) imaging of acetone as a tracer in the fuel supply, while 100 kHz chemiluminescence imaging of excited-state hydroxyl (OH*) radicals simultaneously resolves the evolution of the detonation wave. Time sequences are acquired over multiple detonation cycles in each test, with acetone-PLIF images collected along multiple orthogonal planes to reveal the complex three-dimensional topography of the fuel distribution. The instantaneous and phase averaged acetone-PLIF images enable measurement of key fuel injection characteristics, such as the injector recovery time, fuel jet velocity, and refill height for a range of operating conditions. Instantaneous and phase-averaged measurements of acetone-PLIF with the time-coincident OH* chemiluminescence images also reveal a number key features, such as fuel stratification and weak detonation in the injector near field, incomplete combustion and deflagration behind the detonation wave, vitiation and deflagration of reactants ahead of the detonation wave, and fuel and oxidizer recovery time mismatch leading to combustion inefficiency. These measurements significantly enhance the ability to obtain detailed information on the intracycle and intercycle spatiotemporal evolution of the reactant refill process and its coupled effects on the detonation wave structure and propagation.

Propulsion↗
NASA NTRSDate not supplied

Time-Resolved OH-PLIF Assessment of Deflagrations Levels in a CH4-O2 Rotating Detonation Rocket Combustor

The parasitic loss incurred by deflagrative pre-burning is considered to be one of the key challenges to the effective implementation of rotating detonation engine (RDE) systems. Thus far, there have been relatively limited high fidelity, spatio-temporally resolved measurements of the pre-burning process as a function of the reactant conditions and composition within an RDE. In this work, simultaneous high-repetition-rate broadband OH* chemiluminescence and OH planar laser-induced fluorescence (PLIF) imaging are employed to investigate deflagrative burning dynamics in a fully optically accessible CH4-O2 RDE as compared to a H2-air systems under the same conditions. A custom-built optical parametric oscillator (OPO) is coupled with a high-repetition-rate burst-mode laser to generate the 284 nm source for the excitation of the Q1(9) transition of the OH radical. Significant deflagrative burning is observed throughout the chamber as a consequence of the oxygen-rich environment. Trailing wave systems that consume unburned reactants in the region immediately following the primary detonation wave are observed. The formation of a product gas recirculation zone that entrains and combusts incoming reactants is observed, and quantitative analysis is performed to gain valuable insight into deflagration characteristics. At an oxidizer mass flux of Gox ≈ 350kg/m2/s, it is found that the levels of pre-wave burning in CH4-O2 are a factor of ∼2-3 times higher than in H2-air in the region directly ahead of the detonation wave at a global equivalence ratio Φ ≈ 1.0. This study highlights the key roles of ignition delay and turbulent mixing of the combustion product and reactant fields on the levels of pre-burning and establishes a methodology for further investigation, such as for the effects of various RDE inlet configurations.

Propulsion↗
DOE OSTI2024

A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging

Abstract Genetically encoded fluorescent calcium indicators allow cellular-resolution recording of physiology. However, bright, genetically targetable indicators that can be multiplexed with existing tools in vivo are needed for simultaneous imaging of multiple signals. Here we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several that efficiently label the brain in animals. When bound to a near-infrared dye-ligand, WHaloCaMP shows a 7× increase in fluorescence intensity and a 2.1-ns increase in fluorescence lifetime upon calcium binding. We use WHaloCaMP1a to image Ca 2+ responses in vivo in flies and mice, to perform three-color multiplexed functional imaging of hundreds of neurons and astrocytes in zebrafish larvae and to quantify Ca 2+ concentration using fluorescence lifetime imaging microscopy (FLIM).

Biochemistry & Molecular Biology↗
NASA NTRS1981

Ultraviolet /UV/ sensitive phosphors for silicon imaging detectors

The fluorescence properties of UV sensitive organic phosphors and the radiometric properties of phosphor coated silicon detectors in the VUV, UV, and visible wavelengths are described. With evaporated films of coronene and liumogen, effective quantum efficiencies of up to 20% have been achieved on silicon photodiodes in the vacuum UV. With thin films of methylmethacrylate (acrylic), which are doped with organic laser dyes and deposited from solution, detector quantum efficiencies of the order of 15% for wavelengths of 120-165 nm and of 40% for wavelengths above 190 nm have been obtained. The phosphor coatings also act as antireflection coatings and thereby enhance the response of coated devices throughout the visible and near IR.

Viehmann, W.↗
DOE OSTI2024

Conical Intersection Accessibility Dictates Brightness in Red Fluorescent Proteins

Red fluorescent protein (RFP) variants are highly sought after for in-vivo imaging since longer wavelengths improve depth and contrast in fluorescence imaging. However, the lower energy emission wavelength usually correlates with a lower fluorescent quantum yield compared to their green emitting counterparts. To guide the rational design of bright variants, we have theoretically assessed two variants (mScarlet and mRouge) which are reported to have very different brightness. Using an α-CASSCF QM/MM framework (chromophore and all protein residues within 6 Å of it in the QM region, for a total of more than 450 QM atoms), we identify key points on the ground and first excited state potential energy surfaces. The brighter variant mScarlet has a rigid scaffold, and the chromophore stays largely planar on the ground state. The dimmer variant mRouge shows more flexibility and can accommodate a pre-twisted chromophore conformation which provides easier access to conical intersections. Notably, the main difference between the variants lies in the intersection seam regions, which appear largely inaccessible in mScarlet but partially accessible in mRouge. This observation is mainly related with changes in the cavity charge distribution, the hydrogen-bonding network involving the chromophore and a key ARG/THR mutation (which changes both charge and steric hindrance).

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1994

Spectral imaging of the Orion Bar at 3.3, 8.4, and 11.3 microns: Comparison with a fluorescent polycyclic aromatic hydrocarbon model

Spectral images were obtained of the Orion Bar which sample polycyclic aromatic hydrocarbon (PAH) emission at 3.3, 8.4, and 11.3 micrometers. The images are strikingly different even though they all sample PAH emission. In particular, the 3.3 and 11.3 micrometers images sample PAH emission from C-H bonds, yet the 3.3 micrometers image contains many small bright knots while the 11.3 micrometers image is much more uniform. For comparison with a fluorescent PAH model, a data set was created from the measured intensities of 250 locations in each image. From the comparison, we conclude that: (1) the size distribution of PAHs varies within the Bar, with the bright 3.3 micrometers knots containing the largest proportion of small PAHs; (2) the points along the front of the Bar have emission cross sections characteristic of neutral PAHs while within the Bar, the emission cross sections are different, consistent with the PAHs being charged; (3) the PAHs along the front of the Bar are larger than average for the Bar; (4) emission along the back of the Bar is consistent with PAH emission in an attenuated UV radiation field; (5) there is no evidence for PAH dehydrogenation.

Bregman, Jesse↗
NASA NTRS1994

Electron-Beam Diagnostic Methods for Hypersonic Flow Diagnostics

The purpose of this work was the evaluation of the use of electron-bean fluorescence for flow measurements during hypersonic flight. Both analytical and numerical models were developed in this investigation to evaluate quantitatively flow field imaging concepts based upon the electron beam fluorescence technique for use in flight research and wind tunnel applications. Specific models were developed for: (1) fluorescence excitation/emission for nitrogen, (2) rotational fluorescence spectrum for nitrogen, (3) single and multiple scattering of electrons in a variable density medium, (4) spatial and spectral distribution of fluorescence, (5) measurement of rotational temperature and density, (6) optical filter design for fluorescence imaging, and (7) temperature accuracy and signal acquisition time requirements. Application of these models to a typical hypersonic wind tunnel flow is presented. In particular, the capability of simulating the fluorescence resulting from electron impact ionization in a variable density nitrogen or air flow provides the capability to evaluate the design of imaging instruments for flow field mapping. The result of this analysis is a recommendation that quantitative measurements of hypersonic flow fields using electron-bean fluorescence is a tractable method with electron beam energies of 100 keV. With lower electron energies, electron scattering increases with significant beam divergence which makes quantitative imaging difficult. The potential application of the analytical and numerical models developed in this work is in the design of a flow field imaging instrument for use in hypersonic wind tunnels or onboard a flight research vehicle.

Source record↗
NASA NTRS2000

Non-Intrusive, Laser-Based Imaging of Jet-A Fuel Injection and Combustion Species in High Pressure, Subsonic Flows

The emphasis of combustion research efforts at NASA Glenn Research Center (GRC) is on collaborating with industry to design and test gas-turbine combustors and subcomponents for both sub- and supersonic applications. These next-generation aircraft combustors are required to meet strict international environmental restrictions limiting emissions. To meet these goals, innovative combustor concepts require operation at temperatures and pressures far exceeding those of current designs. New and innovative diagnostic tools are necessary to characterize these flow streams since existing methods are inadequate. The combustion diagnostics team at GRC has implemented a suite of highly sensitive, nonintrusive optical imaging methods to diagnose the flowfields of these new engine concepts. By using optically accessible combustors and flame-tubes, imaging of fuel and intermediate combustion species via planar laser-induced fluorescence (PLIF) at realistic pressures are now possible. Direct imaging of the fuel injection process through both planar Mie scattering and PLIF methods is also performed. Additionally, a novel combination of planar fuel fluorescence imaging and computational analysis allows a 3-D examination of the flowfield, resulting in spatially and temporally resolved fuel/air volume distribution maps. These maps provide detailed insight into the fuel injection process at actual conditions, thereby greatly enhancing the evaluation of fuel injector performance and other combustion phenomena. Stable species such as CO2, O2, N2, H2O, and hydrocarbons are also investigated by a newly demonstrated 1-D, spontaneous Raman spectroscopic method. This visible wavelength Raman technique allows the acquisition of quantitative, stable species concentration measurements from the flow.

Locke, R. J.↗
NASA NTRS2001

Non-Intrusive, Laser-Based Imaging of Jet-A Fuel Injection and Combustion Species in High Pressure, Subsonic Flows

The emphasis of combustion research efforts at NASA Glenn Research Center (GRC) is on collaborating with industry to design and test gas-turbine combustors and subcomponents for both sub- and supersonic applications. These next-generation aircraft combustors are required to meet strict international environmental restrictions limiting emissions. To meet these goals, innovative combustor concepts require operation at temperatures and pressures far exceeding those of cur-rent designs. New and innovative diagnostic tools are necessary to characterize these flow streams since existing methods are inadequate. The combustion diagnostics team at GRC has implemented a suite of highly sensitive, nonintrusive optical imaging methods to diagnose the flowfields of these new engine concepts. By using optically accessible combustors and flametubes, imaging of fuel and intermediate combustion species via planar laser-induced fluorescence (PLIF) at realistic pressures are now possible. Direct imaging of the fuel injection process through both planar Mie scattering and PLIF methods is also performed. Additionally, a novel combination of planar fuel fluorescence imaging and computational analysis allows a 3-D examination of the flowfield, resulting in spatially and temporally resolved fuel/air volume distribution maps. These maps provide detailed insight into the fuel injection process at actual conditions, thereby greatly enhancing the evaluation of fuel injector performance and other combustion phenomena. Stable species such as CO2, O2, N2O. and hydrocarbons are also investigated by a newly demonstrated 1-D, spontaneous Raman spectroscopic method. This visible wavelength Raman technique allows the acquisition of quantitative. stable species concentration measurements from the flow.

Locke, Randy J.↗
NASA NTRS2011

NO PLIF Imaging in the CUBRC 48 Inch Shock Tunnel

Nitric Oxide Planar Laser-Induced Fluorescence (NO PLIF) imaging is demonstrated at a 10 kHz repetition rate in the Calspan-University at Buffalo Research Center s (CUBRC) 48-inch Mach 9 hypervelocity shock tunnel using a pulse burst laser-based high frame rate imaging system. Sequences of up to ten images are obtained internal to a supersonic combustor model, located within the shock tunnel, during a single approx.10-millisecond duration run of the ground test facility. This represents over an order of magnitude improvement in data rate from previous PLIF-based diagnostic approaches. Comparison with a preliminary CFD simulation shows good overall qualitative agreement between the prediction of the mean NO density field and the observed PLIF image intensity, averaged over forty individual images obtained during several facility runs.

Jiang, N.↗
DOE OSTI2024

High-Fidelity Velocity and Concentration Measurements of Turbulent Buoyant Jets

Accurate models of turbulent buoyant flows are essential for the design of the cooling circuit of nuclear reactors and passive safety systems. However, available models fail to fully capture the physics of turbulent mixing when buoyancy becomes predominant with respect to momentum. Therefore, high-fidelity experiments of well-controlled fundamental flows are needed to develop and validate more accurate models. We analyze experiments of positive and negative turbulent buoyant jets, both in uniform and stratified environments, with the aim of understanding the thermal hydraulics of turbulent mixing with variable density and providing high-fidelity data for the development and validation of turbulence models. Non-intrusive, simultaneous particle image velocimetry and laser-induced fluorescence measurements were carried out to acquire instantaneous velocity and concentration fields on a vertical section parallel to the axis of a jet in the self-similar region. The refractive index matching method was applied to measure high-resolution buoyant jets with up to 8.6% density difference. These data are free of the typical errors that characterize optical measurements of buoyancy-driven flows (e.g. natural and mixed convection) where the refractive index of the fluid is inhomogeneous throughout the measurement domain. Turbulent statistics and entrainment of buoyant jets in uniform and stratified environments are presented. These data are compared with non-buoyant jets in a uniform environment, as a reference to investigate the effects of buoyancy and stratification on turbulent mixing. The results will be used for the assessment of current turbulence models and as a basis for the development of a new model that captures turbulent mixing.

laser induced fluorescence↗
DOE OSTI2026

Toward Intelligent Multimodal Holography for Real-Time Chemical Imaging of Dynamic Ion Separation

Molecular-level visualization of ion transport and separation dynamics in complex environments is crucial for advancing energy systems, water purification, and critical materials recovery. Achieving this requires imaging platforms that combine structural sensitivity, chemical specificity, and real-time operation. Digital off-axis holography (DOAH) provides high-throughput, label-free quantitative phase imaging but inherently lacks chemical selectivity. Integrating DOAH with complementary spectroscopic channels such as fluorescence or hyperspectral imaging introduces the needed molecular specificity, while also creating challenges in multimodal data fusion, synchronization, and computational throughput. Artificial intelligence offers a powerful route to address these limitations by uniting physics-based reconstruction with data-driven interpretation. In this Perspective, we outline a framework for intelligent multimodal holography and demonstrate its potential using a preliminary AI-driven test case. Raw DOAH holograms of lanthanide solutions subjected to magnetic field gradients were analyzed using multi-agent AI workflows that autonomously selected reconstruction tools, extracted NMF components, and generated scientific claims consistent with true paramagnetic and diamagnetic behavior. This demonstration shows how AI-enabled reasoning can deliver real-time chemical–structural interpretation directly from raw holograms. Together, these advances define a path toward adaptive, intelligent holography platforms capable of supporting in situ chemical separations, dynamic ion transport analysis, and next-generation interfacial science.

Ricchiuti, Giovanna↗
NASA NTRS2002

A comparison of imaging methods for use in an array biosensor

An array biosensor has been developed which uses an actively-cooled, charge-coupled device (CCD) imager. In an effort to save money and space, a complementary metal-oxide semiconductor (CMOS) camera and photodiode were tested as replacements for the cooled CCD imager. Different concentrations of CY5 fluorescent dye in glycerol were imaged using the three different detection systems with the same imaging optics. Signal discrimination above noise was compared for each of the three systems.

Evaluation Studies↗