Results for “cell envelope” — Page 10

DOE OSTI2020

A small-angle neutron scattering study of the physical mechanism that drives the action of a viral fusion peptide

Viruses have evolved a variety of ways for delivering their genetic cargo to a target cell. One mechanism relies on a short sequence from a protein of the virus that is referred to as a fusion peptide. In some cases, the isolated fusion peptide is also capable of causing membranes to fuse. Infection by HIV-1 involves the 23 amino acid N-terminal sequence of its gp41 envelope protein, which is capable of causing membranes to fuse by itself, but the mechanism by which it does so is not fully understood. In this study, a variant of the gp41 fusion peptide that does not strongly promote fusion was studied in the presence of vesicles composed of a mixture of unsaturated lipids and cholesterol by small-angle neutron scattering and circular dichroism spectroscopy to improve the understanding of the mechanism that drives vesicle fusion. The peptide concentration and cholesterol content govern both the peptide conformation and its impact on the bilayer structure. The results indicate that the mechanism that drives vesicle fusion by the peptide is a strong distortion of the bilayer structure by the peptide when it adopts the β-sheet conformation.

60 APPLIED LIFE SCIENCES↗

NASA NTRS1992

Phase noise in pulsed Doppler lidar and limitations on achievable single-shot velocity accuracy

The smaller sampling volumes afforded by Doppler lidars compared to radars allows for spatial resolutions at and below some sheer and turbulence wind structure scale sizes. This has brought new emphasis on achieving the optimum product of wind velocity and range resolutions. Several recent studies have considered the effects of amplitude noise, reduction algorithms, and possible hardware related signal artifacts on obtainable velocity accuracy. We discuss here the limitation on this accuracy resulting from the incoherent nature and finite temporal extent of backscatter from aerosols. For a lidar return from a hard (or slab) target, the phase of the intermediate frequency (IF) signal is random and the total return energy fluctuates from shot to shot due to speckle; however, the offset from the transmitted frequency is determinable with an accuracy subject only to instrumental effects and the signal to noise ratio (SNR), the noise being determined by the LO power in the shot noise limited regime. This is not the case for a return from a media extending over a range on the order of or greater than the spatial extent of the transmitted pulse, such as from atmospheric aerosols. In this case, the phase of the IF signal will exhibit a temporal random walk like behavior. It will be uncorrelated over times greater than the pulse duration as the transmitted pulse samples non-overlapping volumes of scattering centers. Frequency analysis of the IF signal in a window similar to the transmitted pulse envelope will therefore show shot-to-shot frequency deviations on the order of the inverse pulse duration reflecting the random phase rate variations. Like speckle, these deviations arise from the incoherent nature of the scattering process and diminish if the IF signal is averaged over times greater than a single range resolution cell (here the pulse duration). Apart from limiting the high SNR performance of a Doppler lidar, this shot-to-shot variance in velocity estimates has a practical impact on lidar design parameters. In high SNR operation, for example, a lidar's efficiency in obtaining mean wind measurements is determined by its repetition rate and not pulse energy or average power. In addition, this variance puts a practical limit on the shot-to-shot hard target performance required of a lidar.

Mcnicholl, P.↗

NASA NTRS1987

An analysis of artificial viscosity effects on reacting flows using a spectral multi-domain technique

Standard techniques used to model chemically-reacting flows require an artificial viscosity for stability in the presence of strong shocks. The resulting shock is smeared over at least three computational cells, so that the thickness of the shock is dictated by the structure of the overall mesh and not the shock physics. A gas passing through a strong shock is thrown into a nonequilibrium state and subsequently relaxes down over some finite distance to an equilibrium end state. The artificial smearing of the shock envelops this relaxation zone which causes the chemical kinetics of the flow to be altered. A method is presented which can investigate these issues by following the chemical kinetics and flow kinetics of a gas passing through a fully resolved shock wave at hypersonic Mach numbers. A nonequilibrium chemistry model for air is incorporated into a spectral multidomain Navier-Stokes solution method. Since no artificial viscosity is needed for stability of the multidomain technique, the precise effect of this artifice on the chemical kinetics and relevant flow features can be determined.

Macaraeg, M. G.↗

NASA NTRS1989

Analysis of artificial viscosity effects on reacting flows using a spectral multidomain technique

Standard techniques used to model chemically-reacting flows require an artificial viscosity for stability in the presence of strong shocks. The resulting shock is smeared over at least three computational cells, so that the thickness of the shock is dictated by the structure of the overall mesh and not the shock physics. A gas passing through a strong shock is thrown into a nonequilibrium state and subsequently relaxes down over some finite distance to an equilibrium end state. The artificial smearing of the shock envelops this relaxation zone which causes the chemical kinetics of the flow to be altered. A method is presented which can investigate these issues by following the chemical kinetics and flow kinetics of a gas passing through a fully resolved shock wave at hypersonic Mach numbers. A nonequilibrium chemistry model for air is incorporated into a spectral multidomain Navier-Stokes solution method. Since no artificial viscosity is needed for stability of the multidomain technique, the precise effect of this artifice on the chemical kinetics and relevant flow features can be determined.

Macaraeg, Michele G.↗

DOE OSTI2025

On the role of self-generated magnetic fields in focused ion beams driven by intense picosecond laser pulses

We investigate the physics of ion beam focusing in the interaction of intense picosecond laser pulses with concave foils, using two-dimensional particle-in-cell simulations. Curved targets have been observed to deliver brighter multi-MeV ion beams than flat targets, and they could be used to ignite pre-compressed ICF fusion capsules in a scheme called ion fast ignition. Focusing is achieved by shaping the target and relying on an ion acceleration mechanism in which the ions are accelerated normal to the target surface; subsequently, the plasma expansion grows magnetic fields that advect with the plasma and deflect ions away from the target symmetry axis, causing the focal spot to shift downstream from its geometric location. The drop in both electric and magnetic field strengths in the expanding plasma makes the ion beam envelope asymmetric with respect to the focusing plane.

Focused ion beam↗

NASA NTRS2017

The Lightweight Integrated Solar Array and anTenna (LISA-T) - Big Power for Small Spacecraft

NASA is developing a space power system using lightweight, flexible photovoltaic devices originally developed for use here on Earth to provide low cost power for spacecraft. The Lightweight Integrated Solar Array and anTenna (LISA-T) is a launch-stowed, orbit-deployed array on which thin-film photovoltaic and antenna elements are embedded. The LISA-T system is deployable, building upon NASA's expertise in developing thin-film deployable solar sails such the one being developed for the Near Earth Asteroid Scout project which will fly in 2018. One of the biggest challenges for the NEA Scout, and most other spacecraft, is power. There simply isn't enough of it available, thus limiting the range of operation of the spacecraft from the Sun (due to the small surface area available for using solar cells), the range of operation from the Earth (low available power with inherently small antenna sizes tightly constrain the bandwidth for communication), and the science (you can only power so many instruments with limited power). The LISA-T has the potential to mitigate each of these limitations. Inherently, small satellites are limited in surface area, volume, and mass allocation; driving competition between their need for power and robust communications with the requirements of the science or engineering payload they are developed to fly. LISA-T is addressing this issue, deploying large-area arrays from a reduced volume and mass envelope - greatly enhancing power generation and communications capabilities of small spacecraft and CubeSats. The problem is that these CubeSats can usually only generate between 7 watts and 50 watts of power. The power that can be generated by the LISA-T ranges from tens of watts to several hundred watts. A matrix of options are in development, including planar (pointed) and omnidirectional (non-pointed) arrays. The former is seeking the highest performance possible while the latter is seeking GN&C (Guidance, Navigation and Control) simplicity. In both cases, power generation ranges from tens of watts to several hundred with an expected specific power greater than 250 watts per kilogram and a stowed power density greater than 200 kilowatts per cubic meter. Options for leveraging both high performance, 'typical cost' triple junction thin-film solar cells as well as moderate performance, low cost cells are being developed. Alongside, both UHF (ultra high frequency) and S-band antennas are being integrated into the array to move their space claim away from the spacecraft and open the door for omnidirectional communications and electronically steered phase arrays.

Johnson, Les↗

DOE OSTI2024

Positioning Accuracy in a Concurrent Robot-CNC Hybrid Manufacturing System

Abstract Additive manufacturing (AM) has gained notoriety for offering advantages over traditional manufacturing methods, such as increased design complexity and flexibility. However, it has not found widespread use beyond rapid prototyping. One hindrance to the acceptance of AM processes in industry is the time and cost of fabrication per component. While metal AM by itself can be inexpensive, extra manufacturing steps in the form of subtractive manufacturing (SM) may need to be performed to reach final part tolerances, leading to hybrid additive-subtractive manufacturing (HASM) of a part, which increases time and cost. A potential area to reduce cost is through increasing the efficiency of the HASM process by conducting additive and subtractive manufacturing simultaneously. Usually, HASM is performed in a process where AM is completed in one machine or cell and transferred to another machine or cell for SM in a sequential assembly line process. This efficiency decreases part cost, but high aspect ratio parts or parts with internal geometry that require interleaved additive deposition and machining cannot be produced. One unexplored solution to simultaneous HASM that allows for interleaved operations is to operate the deposition head and machining spindle concurrently within the same machine envelope, known as concurrent HASM (CHASM). In this type of process, both AM and SM occur simultaneously on a batch of small parts or a single large part, maintaining a high efficiency without sacrificing the full range of complex geometries that AM allows for. A potential approach to the single-machine method could be to combine a robot and mill within the same envelope. A challenge to this approach, however, is control of both systems. Most machine controllers have limited external communication or, if a robot has been integrated, only offer movement of either the robot or mill at any given time. As a result, systems must pause either the AM or SM process to switch between them rather than working simultaneously. The present work investigates the positional accuracy of such a CHASM system comprised of a robotic arm and a 3-axis mill. Open-loop tests with limited communication between machines are performed on the system to verify positional error during concurrent robot-mill movements. Under certain conditions, it is demonstrated that position error can stay within 2 mm for the duration of a single layer; however, these tests show that, generally, the open-loop positioning performance of the system is inadequate for CHASM without part-specific hand-tuning of parameters. Based on these results, a set of requirements for successful robot-CNC CHASM is proposed for future integrations.

Goodwin, Jesse↗

NASA NTRS1990

Low thrust rocket test facility

A low thrust chemical rocket test facility has recently become operational at the NASA-Lewis. The new facility is used to conduct both long duration and performance tests at altitude over a thruster's operating envelope using hydrogen and oxygen gas for propellants. The facility provides experimental support for a broad range of objectives, including fundamental modeling of fluids and combustion phenomena, the evaluation of thruster components, and life testing of full rocket designs. The major mechanical and electrical systems are described along with aspects of the various optical diagnostics available in the test cell. The electrical and mechanical systems are designed for low down time between tests and low staffing requirements for test operations. Initial results are also presented which illustrate the various capabilities of the cell.

Arrington, Lynn A.↗

NASA NTRS1990

Low thrust rocket test facility

A low thrust chemical rocket test facility has recently become operational at the NASA-Lewis. The new facility is used to conduct both long duration and performance tests at altitude over a thruster's operating envelope using hydrogen and oxygen gas for propellants. The facility provides experimental support for a broad range of objectives, including fundamental modeling of fluids and combustion phenomena, the evaluation of thruster components, and life testing of full rocket designs. The major mechanical and electrical systems are described along with aspects of the various optical diagnostics available in the test cell. The electrical and mechanical systems are designed for low down time between tests and low staffing requirements for test operations. Initial results are also presented which illustrate the various capabilities of the cell.

Arrington, Lynn A.↗

NASA NTRS2015

Synthetic Biology and the Search for Extraterrestrial Life

"Are we alone?" is one of the primary questions of astrobiology, and whose answer defines our significance in the universe. Unfortunately, this quest is hindered by the fact that we have only one confirmed example of life, that of earth. While this is enormously helpful in helping to define the minimum envelope for life, it strains credulity to imagine that life, if it arose multiple times, has not taken other routes. To help fill this gap, our lab has begun using synthetic biology - the design and construction of new biological parts and systems and the redesign of existing ones for useful purposes - as an enabling technology. One theme, the "Hell Cell" project, focuses on creating artificial extremophiles in order to push the limits for Earth life, and to understand how difficult it is for life to evolve into extreme niches. In another project, we are re-evolving biotic functions using only the most thermodynamically stable amino acids in order to understand potential capabilities of an early organism with a limited repertoire of amino acids.

amino acids↗

NASA NTRS2015

Synthetic Biology and the Search for Extraterrestrial Life

"Are we alone?" is one of the primary questions of astrobiology, and whose answer defines our significance in the universe. Unfortunately, this quest is hindered by the fact that we have only one confirmed example of life, that of earth. While this is enormously helpful in helping to define the minimum envelope for life, it strains credulity to imagine that life, if it arose multiple times, has not taken other routes. To help fill this gap, our lab has begun using synthetic biology - the design and construction of new biological parts and systems and the redesign of existing ones for useful purposes - as an enabling technology. One theme, the "Hell Cell" project, focuses on creating artificial extremophiles in order to push the limits for Earth life, and to understand how difficult it is for life to evolve into extreme niches. In another project, we are re-evolving biotic functions using only the most thermodynamically stable amino acids in order to understand potential capabilities of an early organism with a limited repertoire of amino acids.

exobiology↗

NASA NTRS2015

Synthetic Astrobiology

'Are we alone?' is one of the primary questions of astrobiology, and whose answer defines our significance in the universe. Unfortunately, this quest is hindered by the fact that we have only one confirmed example of life, that of earth. While this is enormously helpful in helping to define the minimum envelope for life, it strains credulity to imagine that life, if it arose multiple times, has not taken other routes. To help fill this gap, our lab has begun using synthetic biology - the design and construction of new biological parts and systems and the redesign of existing ones for useful purposes - as an enabling technology. One theme, the "Hell Cell" project, focuses on creating artificial extremophiles in order to push the limits for Earth life, and to understand how difficult it is for life to evolve into extreme niches. In another project, we are re-evolving biotic functions using only the most thermodynamically stable amino acids in order to understand potential capabilities of an early organism with a limited repertoire of amino acids.

universe↗

NASA NTRS2017

Synthetic Astrobiology

"Are we alone?" is one of the primary questions of astrobiology, and whose answer defines our significance in the universe. Unfortunately, this quest is hindered by the fact that we have only one confirmed example of life, that of earth. While this is enormously helpful in helping to define the minimum envelope for life, it strains credulity to imagine that life, if it arose multiple times, has not taken other routes. To help fill this gap, our lab has begun using synthetic biology - the design and construction of new biological parts and systems and the redesign of existing ones for useful purposes - as an enabling technology. One theme, the "Hell Cell" project, focuses on creating artificial extremophiles in order to push the limits for Earth life, and to understand how difficult it is for life to evolve into extreme niches. In another project, we are re-evolving biotic functions using only the most thermodynamically stable amino acids in order to understand potential capabilities of an early organism with a limited repertoire of amino acids.

universe↗

NASA NTRS2014

Synthetic Biology and the Search for Extraterrestrial Life

Are we alone? is one of the primary questions of astrobiology, and whose answer defines our significance in the universe. Unfortunately, this quest is hindered by the fact that we have only one confirmed example of life, that of earth. While this is enormously helpful in helping to define the minimum envelope for life, it strains credulity to imagine that life, if it arose multiple times, has not taken other routes. To help fill this gap, our lab has begun using synthetic biology, the design and construction of new biological parts and systems and the redesign of existing ones for useful purposes, as an enabling technology. One theme, the Hell Cell project, focuses on creating artificial extremophiles in order to push the limits for Earth life, and to understand how difficult it is for life to evolve into extreme niches (http:2012.igem.orgTeam:Stanford-BrownHellCellIntroduction). In another project, we are re-evolving biotic functions using only the most thermodynamically stable amino acids in order to understand potential capabilities of an early organism with a limited repertoire of amino acids. This should lead to a more universal theory of the origin of life based on materials found commonly in meteorites and other pre-biotic settings.

Hell Cell↗

NASA NTRS1995

Micromechanical models for textile structural composites

The objective is to develop micromechanical models for predicting the stiffness and strength properties of textile composite materials. Two models are presented to predict the homogeneous elastic constants and coefficients of thermal expansion of a textile composite. The first model is based on rigorous finite element analysis of the textile composite unit-cell. Periodic boundary conditions are enforced between opposite faces of the unit-cell to simulate deformations accurately. The second model implements the selective averaging method (SAM), which is based on a judicious combination of stiffness and compliance averaging. For thin textile composites, both models can predict the plate stiffness coefficients and plate thermal coefficients. The finite element procedure is extended to compute the thermal residual microstresses, and to estimate the initial failure envelope for textile composites.

Marrey, Ramesh V.↗

NASA NTRS1989

Rain core structure statistics derived from radar and disdrometer measurements in the mid-Atlantic coast of the US

During a period spanning more than 5 years, low elevation radar measurements of rain were systematically obtained in the mid-Atlantic coast of the U.S. Drop size distribution measurements with a disdrometer were also acquired on the same rain days. The drop size data were utilized to convert the radar reflectivity factors to estimated rain rates for the respective rain days of operation. Applying high level algorithms to the rain data, core values of rain intensities were identified (peak rain rates), and families of rain rate isopleths analyzed. In particular, equicircle diameters of the family of isopleths enveloping peak rain intensities were statistically characterized. The presented results represents the analysis of two rain days, 12 radar scans, corresponding to 430 culled rain rate isopleths from an available data base of 22,000 contours, approximately 100 scans encompassing 17 rain days. The results presented show trends of the average rain rate vs. contour scale dimensions, and cumulative distributions of rain cell dimensions which belong to core families of precipitation.

Goldhirsh, Julius↗

NASA NTRS2003

Furrow microtubules and localized exocytosis in cleaving Xenopus laevis embryos

In dividing Xenopus eggs, furrowing is accompanied by expansion of a new domain of plasma membrane in the cleavage plane. The source of the new membrane is known to include a store of oogenetically produced exocytotic vesicles, but the site where their exocytosis occurs has not been described. Previous work revealed a V-shaped array of microtubule bundles at the base of advancing furrows. Cold shock or exposure to nocodazole halted expansion of the new membrane domain, which suggests that these microtubules are involved in the localized exocytosis. In the present report, scanning electron microscopy revealed collections of pits or craters, up to approximately 1.5 micro m in diameter. These pits are evidently fusion pores at sites of recent exocytosis, clustered in the immediate vicinity of the deepening furrow base and therefore near the furrow microtubules. Confocal microscopy near the furrow base of live embryos labeled with the membrane dye FM1-43 captured time-lapse sequences of individual exocytotic events in which irregular patches of approximately 20 micro m(2) of unlabeled membrane abruptly displaced pre-existing FM1-43-labeled surface. In some cases, stable fusion pores, approximately 2 micro m in diameter, were seen at the surface for up to several minutes before suddenly delivering patches of unlabeled membrane. To test whether the presence of furrow microtubule bundles near the surface plays a role in directing or concentrating this localized exocytosis, membrane expansion was examined in embryos exposed to D(2)O to induce formation of microtubule monasters randomly under the surface. D(2)O treatment resulted in a rapid, uniform expansion of the egg surface via random, ectopic exocytosis of vesicles. This D(2)O-induced membrane expansion was completely blocked with nocodazole, indicating that the ectopic exocytosis was microtubule-dependent. Results indicate that exocytotic vesicles are present throughout the egg subcortex, and that the presence of microtubules near the surface is sufficient to mobilize them for exocytosis at the end of the cell cycle.

Non-NASA Center↗

DOE OSTI2025

Graphene reinforced UHMWPE fibers

Thermoplastic polymers are increasingly used in electric vehicles, hydrogen fuel cell vehicles, and other decarbonization applications due to their lightweight and formability. Higher-strength polymers are needed to supplant metals in the vehicle structure, thereby reducing mass and improving efficiency. Ultra-high molecular weight polyethylene (UHMWPE) fibers possess one of the highest strength-to-weight ratios of technical polymers, and further improvement via reinforcement by nanofillers, such as graphene, will expand their performance envelope. Here, in this work, UHMWPE/graphene nanocomposite fibers were gel spun and characterized for their morphological, microstructural, thermal, and mechanical properties. The addition of a low fraction of graphene improved the tensile strength of the fibers by 25% and tensile modulus by 32%. Differential scanning calorimetry showed an increase in melting temperature and degree of crystallinity, which indicates improved coordination of the molecular chains induced by the addition of graphene. The reinforcement also affected the cross-sectional shape of the fibers; the aspect ratio of the fibers’ elliptical shape declined with increasing graphene content showing the skeletal effect of the graphene nanofillers in the polymer matrix. The reinforcing effect of graphene declined above a threshold concentration, and theoretical modeling was applied to demonstrate that increased agglomerates led to reduced properties. This work demonstrates a simple, effective method to produce graphene-reinforced UHMWPE fibers and lays a foundation for understanding the potential for leveraging graphene to form ultra-high-performance nanocomposite fibers for myriad engineering applications.

36 MATERIALS SCIENCE↗