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At least 181 records · Page 10

Vegetation Warming Experiment: 15N Uptake Experiment Arctagrostis latifolia Canopy Traits, Utqiagvik (Barrow), Alaska, 2018

This dataset consists of measured Arctagrostis latifolia canopy traits harvested from vegetation warming experiment enclosures and paired control plots located on the BEO, Utqiavik, Alaska. Harvest types include both natural abundance and enriched A. latifolia. Canopy traits measured include leaf area index (LAI), specific leaf area (SLA) and maximum height. Vegetation warming chambers (Zero Power) were deployed on the Barrow Environmental Observatory (BEO), Utqiavik, Alaska. These chambers (Figure 1) consistently elevated air temperatures by approximately 4°C using a self-venting system described by Lewin et al (2017). Five chambers were deployed from June 17, 2018 to September 24, 2018 on the BEO within a 1 km2 area centered on 71.275N, -156.641W. Each chamber was co-located with an ambient plot where temperatures were not manipulated on patches of tundra containing the target species Arctagrostis latifolia. An intensive field campaign in late July investigated the impact of warming had on A. latifolia biomass, chemistry, and uptake of 15N labeled ammonia that was injected into the surface soils for one week. Initial measurements were taken on July 21, 2018. Harvest occurred on July 27, 2018. Water-extractable nutrients in soils were measured in July following harvests of A. latifolia plants and underlying soils. Availability of ammonia, nitrate, and phosphate throughout the growing season was measured by extracting nutrients bound to anion and cation binding resins deployed from July through September. Environmental variables (thaw depth, surface soil temperatures, surface soil moisture) were measured. Leaf traits and root traits of A. latifolia were also measured. This dataset includes one csv file and one pdf file. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

A quest for porphyrins in lunar soil - Samples from Apollo 11, 12 and 14.

Analyses for porphyrins in the lunar samples were carried out by extracting the lunar soils as received by organic solvents followed by analytical demetallation using methanesulfonic acid after which free-base porphyrins, if present, were recovered and demonstrably recomplexed with divalent cations. Samples from Apollo 11 showed the presence of fluorescent substances attributed to exhaust from the descent engine. One sample from Apollo 12 showed pigments resembling porphyrins. The Apollo 14 results were negative.

Hodgson, G. W.↗

Highly evolved and ultramafic lithologies from Apollo 14 soils

Eighteen 2-4-mm fragments were extracted from several Apollo 14 soil samples and examined by instrumental neutron activation analysis, and were studied petrographically. Results indicate that these rock fragments span almost the whole range of magma evolution, from ultramafic cumulates to extreme differentiates that were apparently produced by silicate liquid immiscibility. Three of the fragments are dimict granitic breccias composed of granitic clasts intruded by a ferropyroxenitic glass, and may represent an immmiscible melt pair that has been remixed by impact. Two fragments are ultramafic rocks, including a dunite with very low REE abundances and a peridotite/impact melt breccia.

Morris, R. W.↗

A Reexamination of Amino Acids in Lunar Soils: Implications for the Survival of Exogenous Organic Material During Impact Delivery

Using a sensitive high performance liquid chromatography technique, we have analyzed both the hot water extract and the acid hydrolyzed hot water extract of lunar soil collected during the Apollo 17 mission. Both free amino acids and those derived from acid labile precursors are present at a level of roughly 15 ppb. Based on the D/L amino acid ratios, the free alanine and aspartic acid observed in the hot water extract can be entirely attributed to terrestrial biogenic contamination. However, in the acid labile fraction, precursors which yield amino acids are apparently present in the lunar soil. The amino acid distribution suggests that the precursor is probably solar wind implanted HCN. We have evaluated our results with regard to the meteoritic input of intact organic compounds to the moon based on an upper limit of less than or equal to 0.3 ppb for alpha-aminoisobutyric acid, a non-protein amino acid which does not generally occur in terrestrial organisms and which is not a major amino acid produced from HCN, but which is a predominant amino acid in many carbonaceous chondrites. We find that the survival of exogenous organic compounds during lunar impact is less than or equal to 0.8%. This result represents an example of minimum organic impact survivability. This is an important first step toward a better understanding of similar processes on Earth and on Mars, and their possible contribution to the budget of prebiotic organic compounds on the primitive Earth.

Brinton, Karen L. F.↗

Taurus Littrow Pyroclastic Deposit-An Optimum Feedstock for Lunar Oxygen

Future human habitation of the Moon will likely require the use of locally derived materials because of the high cost of transportation from Earth. Oxygen, extracted from oxides and silicates, is a potentially abundant lunar resource vital for life support and spacecraft propulsion. The anticipated costs of supplying all oxygen needs for a lunar base from Earth are high enough to warrant serious study of oxygen production from local resources. Over 20 different processes have been proposed for oxygen production on the Moon. Among the simplest and best studied of these processes is the reduction of oxides in lunar minerals and glass using hydrogen gas. Oxygen can be extracted from lunar soils and pyroclastic glass beads by exposing the samples to flowing hydrogen at subsolidus temperatures (approx. 1050 C). Total oxygen yield is directly correlated to the sample's abundance of FeO, but is not correlated to the abundance of any other oxide. Oxygen is extracted predominantly from FeO, with lesser contributions from TiO2 and SiO2. Oxygen yield is independent of soil maturity. All major FeO-bearing phases contribute oxygen, with extraction from ilmenite and glass significantly more efficient than from olivine and pyroxene. This study demonstrates that the optimum location for a lunar resources demonstration mission can be identified, and that the oxygen yield can be predicted, using a combination of high-resolution imaging and thermal-infrared data. A mission to Taurus Littrow will encounter a deposit at least 10 m in depth with few landing hazards, a uniform composition, and a predicted oxygen yield of approximately 3 wt. %, among the highest values on the Moon.

Allen, Carlton C.↗

Fine Root and Soil Organic Carbon Depth Distributions are Inversely Related Across Fertility and Rainfall Gradients in Lowland Tropical Forests

Humid tropical forests contain some of the largest soil organic carbon (SOC) stocks on Earth. Much of this SOC is in subsoil, yet variation in the distribution of SOC through the soil profile remains poorly characterized across tropical forests. We used a correlative approach to quantify relationships among depth distributions of SOC, fine root biomass, nutrients and texture to 1 m depths across 43 lowland tropical forests in Panama. The sites span rainfall and soil fertility gradients, and these are largely uncorrelated for these sites. We used fitted b parameters to characterize depth distributions, where b is a numerical index based on an asymptotic relationship, such that larger b values indicate greater concentrations of root biomass or SOC at depth in the profile. Root b values ranged from 0.82 to 0.95 and were best predicted by soil pH and extractable potassium (K) stocks. For example, the three most acidic (pH < 4) and Kpoor (< 20 g K m -2 ) soils contained 76 ± 5% of fine root biomass from 0 to 10 cm depth, while the three least acidic (pH > 6.0) and most K-rich (> 50 g K m -2 ) soils contained only 41 ± 9% of fine root biomass at this depth. Root b and SOC b values were inversely related, such that a large fine root biomass in surface soils corresponded to large SOC stocks in subsoils (50–100 cm). SOC b values were best predicted by soil pH and base cation stocks, with the three most base-poor soils containing 34 ± 8% of SOC from 50 to 100 cm depth, and the three most base-rich soils containing just 9 ± 2% of SOC at this depth. Nutrient depth distributions were not related to Root b or SOC b values. Here, these data show that large surface root biomass stocks are associated with large subsoil C stocks in strongly weathered tropical soils. Further studies are required to evaluate why this occurs, and whether changes in surface root biomass, as may occur with global change, could in turn influence SOC storage in tropical forest subsoils.

54 ENVIRONMENTAL SCIENCES↗

Soil viruses: Understudied agents of soil ecology

Over the past couple decades there has been intense exploration of the soil microbiome using modern sequencing and bioinformatics approaches. These studies have revealed a vast diversity of microorganisms across a variety of habitats (Thompson et al., 2017) and how differences in the environment, for example with climate change, influence the soil microbial community composition (Jansson and Hofmockel, 2020). The focus of these studies has primarily been on microbial cellular organisms, including bacteria, archaea and fungi. By contrast, in depth study of soil viruses has been largely neglected, until recently. There are several reasons that soil viruses are less studied, including difficulties with extraction of viruses from soil particles, difficulties with classification of soil viruses based on microscopic imaging, and reliance on the small proportion of microbial hosts that can be cultivated. Now that comprehensive soil metagenomes are available the current trend has been to survey the metagenomes for viral sequences. This approach has revealed that soil viruses are incredibly abundant, highly diverse, and largely uncharacterized (Paez-Espino et al., 2016). This so-called ‘viral dark matter’ presents an incredible research opportunity for the future. Here I will highlight some of these potential research avenues based on what is known to date.

54 ENVIRONMENTAL SCIENCES↗

Biological potential of extraterrestrial materials. 2. Microbial and plant responses to nutrients in the Murchison carbonaceous meteorite

Meteoritic materials are investigated as potential early planetary nutrients. Aqueous extracts of the Murchison C2 carbonaceous meteorite are utilized as a sole carbon source by microorganisms, as demonstrated by the genetically modified Pseudomonas fluorescence equipped with the lux gene. Nutrient effects are observed also with the soil microorganisms Nocardia asteroides and Arthrobacter pascens that reach populations up to 5 x 10(7) CFU/ml in meteorite extracts, similar to populations in terrestrial soil extracts. Plant tissue cultures of Asparagus officinalis and Solanum tuberosum (potato) exhibit enhanced pigmentation and some enhanced growth when meteorite extracts are added to partial nutrient media, but inhibited growth when added to full nutrient solution. The meteorite extracts lead to large increases in S, Ca, Mg, and Fe plant tissue contents as shown by X-ray fluorescence, while P, K, and Cl contents show mixed effects. In both microbiological and plant tissue experiments, the nutrient and inhibitory effects appear to be best balanced for growth at about 1:20 (extracted solid : H2O) ratios. The results suggest that solutions in cavities in meteorites can provide efficient concentrated biogenic and early nutrient environments, including high phosphate levels, which may be the limiting nutrient. The results also suggest that carbonaceous asteroid resources can sustain soil microbial activity and provide essential macronutrients for future space-based ecosystems.

Non-NASA Center↗

A Blind Convolutional Deep Autoencoder for Spectral Unmixing of Hyperspectral Images Over Waterbodies

Harmful algal blooms have dangerous repercussions for biodiversity, the ecosystem, and public health. Automatic identification based on remote sensing hyperspectral image analysis provides a valuable mechanism for extracting the spectral signatures of harmful algal blooms and their respective percentage in a region of interest. This paper proposes a new model called a non-symmetrical autoencoder for spectral unmixing to perform endmember extraction and fractional abundance estimation. The model is assessed in benchmark datasets, such as Jasper Ridge and Samson. Additionally, a case study of the HSI2 image acquired by NASA over Lake Erie in 2017 is conducted for extracting optical water types. The results using the proposed model for the benchmark datasets improve unmixing performance, as indicated by the spectral angle distance compared to five baseline algorithms. Improved results were obtained for various metrics. In the Samson dataset, the proposed model outperformed other methods for water (0.060) and soil (0.025) endmember extraction. Moreover, the proposed method exhibited superior performance in terms of mean spectral angle distance compared to the other five baseline algorithms. The non-symmetrical autoencoder for the spectral unmixing approach achieved better results for abundance map estimation, with a root mean square error of 0.091 for water and 0.187 for soil, compared to the ground truth. For the Jasper Ridge dataset, the non-symmetrical autoencoder for the spectral unmixing model excelled in the tree (0.039) and road (0.068) endmember extraction and also demonstrated improved results for water abundance maps (0.1121). The proposed model can identify the presence of chlorophyll-a in waterbodies. Chlorophyll-a is an essential indicator of the presence of the different concentrations of macrophytes and cyanobacteria. The non-symmetrical autoencoder for spectral unmixing achieves a value of 0.307 for the spectral angle distance metric compared to a reference ground truth spectral signature of chlorophyll-a. The source code for the proposed model, as implemented in this manuscript, can be found at https://github.com/EstefaniaAlfaro/autoencoder_owt_spectral.git.

hyperspectral imaging↗

Partitioning sources of CO 2 emission after soil wetting using high-resolution observations and minimal models

Wetting dry soil triggers a pulse of microbial respiration. Respiration pulses after wetting can be complex, evolving through several stages before subsiding—possibly reflecting contributions from multiple carbon (C) sources. In this work we hypothesized that respiration after wetting combines C from cellular sources (notably trehalose, a cellular osmo-protectant) and soluble extracellular C made available by the physical effects of wetting, with cellular sources contributing early during the pulse and extracellular C dominating later. We tested this hypothesis by making measurements of soil respiration, microbial biomass, trehalose, and water extractable organic C after wetting dry soil in the laboratory. We then devised several alternative minimal models to evaluate the potential contributions of trehalose and extracellular C to the respiration pulse. We found that the chloroform-labile fraction of the microbial biomass was comprised of 50% trehalose in dry soil. Both trehalose and water extractable organic C pools declined rapidly after wetting; trehalose disappeared within 3 h of water addition. Chloroform-labile glucose increased 3 h after water addition and then declined, suggesting rapid hydrolysis of trehalose and consumption of the resulting glucose. Respiration dynamics after wetting could be best explained by models that included two C sources, combining rapid metabolism of trehalose with slower consumption of extracellular soluble C. These results suggest that pulses of respiration after wetting reflect both osmolyte consumption within the microbial biomass and enhanced extracellular C availability; hence, models of microbial drought response must account for both processes to fully resolve soil C dynamics.

54 ENVIRONMENTAL SCIENCES↗

The quality of organic amendments affects soil microbiome and nitrogen-cycling bacteria in an organic farming system

Organic amendments are applied in organic farming systems to provide nutrients for crop uptake and to improve soil health. Compost is often favored over fresh manure for food safety reasons, while fresh manure can be a valuable source of readily available nitrogen (N). However, the potential for fresh versus composted manure to differentially affect soil microbial and N-cycling functional communities over multiple seasons remains unknown. We compared the effect of composted vs. fresh cattle manure on soil microbial communities using taxonomic and functional approaches. Soils were collected from field plots with three organic N treatments: control (no amendment), composted manure (compost, 224 kg/ha total N), and fresh manure (manure, 224 kg/ha total N) in an organic production system. Illumina amplicon sequencing was used to comprehensively assess the bacterial community (16S rRNA genes), fungal community (ITS), ureolytic community ( ureC ), chitinolytic community ( chiA ), bacterial ammonia oxidizers (AOB amoA ), and nitrite oxidizers ( Nitrospira nxrB ). The results showed that both compost and manure treatment significantly changed the soil microbial communities. Manure had a stronger effect than compost on soil bacterial and fungal community composition, as well as on the ureolytic and chitinolytic communities, while compost treated soils had higher microbial richness than manure treated soils. Both taxonomic and functional approaches showed that the microbial community was more responsive to fresh manure than to compost. Manure treated soil also had more complex microbial interactions than compost treated soil. The abundance and community composition of N-cycling functional groups often played more limited roles than soil chemical properties (soil organic carbon, extractable organic carbon, and pH) in driving N-cycling processes. Results from our study may guide strategies for the management of organic amendments in organic farming systems and provide insights into the linkages between soil microbial communities and soil function.

Ouyang, Yang↗

Genome collection processing for “Conserved upper thermal limits and small safety margins in soil copiotrophic bacteria”

We extracted the genomic DNA of 400 randomly selected isolates using a Quick-DNA Microprep Kit (Zymo Research D3020) according to the manufacturer’s protocol. We then submitted the extracted gDNA samples for short-read Illumina sequencing (200 Mbp) at SeqCoast Genomics (Portsmouth, NH, USA). After preprocessing the sequences using Trimmommatic (Bolger et al. 2014), we assembled the genomes using SPADES (Bankevich et al. 2012) and checked the quality of each assembly using QUAST (Gurevich et al. 2013). We processed the genome assemblies using a KBase (v1.4.0) pipeline (Allen et al. 2017; Arkin et al. 2018). Briefly, we used DRAM (v0.1.2) with default settings to annotate the genome assemblies. We then evaluated genome quality and possible contamination levels using CheckM (v1.0.18) (Parks et al. 2015) and retained genomes with completeness above 98% and contamination below 5% (n = 354), following the authors' guidelines. We then obtained taxonomic assignments for all remaining isolates using the Genome Taxonomy Database tool GTDB-Tk (v2.3.2, database version r214) (Chaumeil et al. 2019). We constructed a phylogenetic tree using the tool SpeciesTree (v2.2.0). We then trimmed the tree (using Trim SpeciesTree to GenomeSet- v1.4.0), retaining only tips within our collection with measured thermal performance.

59 BASIC BIOLOGICAL SCIENCES↗

Lunar hydrogen: A resource for future use at lunar bases and space activities

Hydrogen abundances were determined for grain size separates of five lunar soils and one soil breccia. The hydrogen abundance studies have provided important baseline information for engineering models undergoing study at the present time. From the studies is appears that there is sufficient hydrogen present in selected lunar materials which could be recovered to support future space activities. It is well known that hydrogen can be extracted from lunar soils by heating between 400 and 800 C. Recovery of hydrogen for regolith materials would involve heating with solar mirrors and collecting the released hydrogen. Current baseline models for the lunar base are requiring the production of 1000 metric tons of oxygen per year. From this requirement it follows that around 117 metric tons per year of hydrogen would be required for the production of water. The ability to obtain hydrogen from the lunar regolith would assist in lowering the operating costs of any lunar base.

Gibson, Everett K., Jr.↗

Biomarkers Detection With the Autonomous and Remotely Operated SOLID-LDChip Instrument in A Mars Drilling Simulating Campaign

One of the main goals in Mars exploration is to determine whether life has ever existed on the red planet. To achieve this goal, it is important to verify the performance, robustness, and maturity of instrumentation devoted to life detection, for example through field-testing in Mars analog environments.The Atacama Desert is considered one of the best terrestrial analogs of the surface of Mars due to abrupt temperature shifts, dryness, high UV radiation, and extremely low biomass. This, together with the lack of vegetation and the geochemistry of the regolith, make it an ideal scenario for testing instrumentation, performance, and concepts of operations. The Atacama Rover Astrobiology Drilling Studies (ARADS) is a NASA PSTAR project conceived for maturing and testing life-detection instrumentation in the Atacama Desert(1). The project is based on the K-REX2 rover equipped with a robotic 1-meter arm that delivers samples toon board instruments. This includes SOLID3.1 (Signs of Life Detector), designed as a life-detection instrument for finding complex organic molecules by means of the LDCHip (Life Detector Chip), an antibody microarray sensor(2). The instrument can extract organic compounds from soil, rocks, and sediments into a liquid solvent in the extraction cell (EC), allowing the search for hundreds of microbial molecular biomarkers at once (3). The robustness and reliability of SOLID were tested in the 2019 ARADS Mars drilling simulating campaign and confirmed with other analytical methods.

M Moreno-Paz↗

Scripts and data associated with a manuscript linking soil and sediment elemental composition with dissolved organic matter chemistry across CONUS

This data package provides scripts and geochemical data for a manuscript titled “Linkages between mineral element composition of soils and sediments with hyporheic zone dissolved organic matter chemistry across the contiguous United States” (preprint: doi: 10.22541/essoar.169447343.31694990/v1). This data is associated with the Worldwide Hydrobiogeochemistry Observation Network for Dynamic River Systems (WHONDRS, https://whondrs.pnnl.gov) and is an extension of the Summer 2019 Sampling campaign which crowdsourced samples from rivers and sediment across the continental United States. Data from this study can be found at https://data.ess-dive.lbl.gov/datasets/doi:10.15485/1603775 and https://data.ess-dive.lbl.gov/datasets/doi:10.15485/1729719. The main objective of this manuscript was to couple sediment water extractable dissolved organic matter chemistry, defined by ultra-high resolution mass spectrometry, with localized sediment elemental composition and watershed scale soil elemental characteristics. This data package contains one main folder with four subfolders. The main data folder contains (1) readme; (2) data dictionary (dd); (3) file-level metadata (flmd); (4) an R markdown to reproduce manuscript figures and analyses; (5) a pdf of instructions to reproduce NGS interpolations with ArcGIS software; and (6) a python script to reproduce NGS extrapolations with python. The four subfolders contain files required to reproduce NGS extrapolations include (1) ‘CONUS_boundaries’ containing boundary layers (.shp) for the Continental United States; (2) ‘ngs_project’ containing files (.shp) with point level NGS soil elemental data (Grossman et al., 2004); (3) ‘raster_outputs’ containing the interpolated raster output files for various soil elements; and (4) ‘NGS_Chemistry_Final’ contain final extracted soil elemental data.

54 ENVIRONMENTAL SCIENCES↗

Application of manufactured products

A wide range of products can be manufactured from the following materials: (1) lunar regolith or basalt; (2) regolith or rock beneficiated to concentrate plagioclase or other minerals; (3) iron, extracted from lunar soil or rocks by various means; (4) naturally occurring or easily obtained materials that have cementitious properties; and (5) byproducts of the above materials. Among the products that can be produced from these materials are the following: beams; plates and sheets; transparent plates (windows); bricks and blocks; pipes and tubes; low-density materials (foams); fiber, wire, and cables; foils and reflective coatings; hermetic seals (coatings); and formed objects. In addition to oxygen, which can be obtained by several processes, either from unbeneficiated regolith or by reduction of concentrated ilmenite, these materials make the simplest requirements of the lunar resource extraction system. A thorough analysis of the impact of these simplest products on the economics of space operations is not possible at this point. Research is necessary both to define optimum techniques and adapt them to space and to determine the probable market for the products so that the priority of various processes can be assessed. Discussions of the following products are presented: aerobraking heat shields; pressurized habitats; lunar photovoltaic farms; and agricultural systems.

Sastri, Sankar↗

207Pb-206Pb Geochronology and Microstructures of Phosphates From Apollo 14 and 15 Soils

Phosphates (apatite and merrillite) are common accessory minerals in many Apollo basalt and regolith samples. Phosphates typically have lower closure temperatures than their companion accessory phase zircon for their U-Pb and (U-Th)/He geochronologic systems [e.g., 1, 2]. As such, the ages of phosphates in impact melt-bearing breccias have typically been used to constrain the ages of impact events [3]. Phosphates have also served as anchors for 207Pb-206Pb isochrons allowing for precise ages of basaltic samples to be determined [4]. Apatite is also a reservoir for volatile species (e.g., F, Cl, OH, S) [e.g., 5, 6] which are important for investigating the formation and evolution of the lunar interior [7-11]. Recent work by [11] and [12] has shown that deformation and exposure to high temperatures, especially those in impact environments, can influence both apatite chronology and chemistry. Here we present the first chronology and microstructure results of a large consortium study aimed at unraveling the impact and endogenous volatile record of phosphates extracted from lunar soils. These soils potentially sample a larger range of lithologies than the rocks returned by Apollo astronauts, and should, therefore, represent a more global phosphate record. For more information on low temperature (U-Th)/He thermochronology, please see the companion abstract [13].

C A Crow↗

Kiloton Class ISRU Systems for LO2/LCH4 Propellant Production on the Mars Surface

As part of the 2023 strategic analysis cycle to explore the trade space, the NASA Mars Architecture Team wanted to explore what it takes to produce in situ on Mars many hundreds of tons of propellants for a large all-chemical transportation system. The conceptual operations and design of the LO 2 /LCH 4 in situ resource utilization (ISRU) water acquisition, propellant production and liquification system was assigned to the NASA Compass concurrent engineering team with support from various NASA ISRU, cryogenic fluid management, and surface power experts. The conceptual point design examined one case producing 300 t of LO 2 /LCH 4 from the Mars atmosphere and delivered water in 20 months and storing the liquified propellants in a to-be-reused lander. Several of these large single-stage, all-chemical class large vertical landers would deliver the required ISRU equipment. The required 150 t of water stock for the ISRU system was traded between three options: delivered, pumped from subsurface ice deposits or extracted from surface soils. The large propellant production systems consist of atmospheric CO 2 collection scroll pumps, a combined solid oxide electrolysis and methanation system to convert the CO 2 and water into gaseous O 2 and CH 4 , and various dryers, scrubbers, and separators to remove the excess water, CO 2 and H 2 . The liquefaction system consisted of 90 K cryocoolers to provide cold Ne to the launch vehicle tanks to liquify these CH 4 and O 2 gases and store them as rocket propellants. The systems are deployed using a 6 t (payload) capable chassis derived from conceptual pressurized rover designs. In total, the propellant production and liquefaction systems required three propellant production pallets, two liquefaction pallets, two water tankers, and six 40 kW-fission surface power systems (FSPS) with cabling. All this equipment was found to notionally fit inside two- 75 metric ton payload capacity Mars ascent and landing vehicles (MALV). For the case where 150 t of water delivered from Earth, four cargo MALVs are required for the full system. The same is true when the 150 t of water is extracted through surface mining. For the borehole system, only 3 cargo MALVs are necessary. A comparison of approaches in terms of number of landers, number and type of elements, power and time is made.

ISRU↗