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Uses of Computed Tomography in the NASA Materials Science Program

Computed Tomography (CT) has proved to be of inestimable use in providing a rapid evaluation of a variety of samples from Mechanics of Granular Materials (MGM) to electronic materials (Ge-Si alloys) to space grown materials such as meteorites. The system at Kennedy Space Center (KSC), because of its convenient geographical location, is ideal for examining samples immediately after returning to Earth. It also has the advantage of the choice of fluxes, and in particular the use of a radioactive cobalt source, which is basically monochromatic. This permits a reasonable measurement of density to be made from which chemical composition can be determined. Due to the current dearth of long duration space grown materials, the CT instrument has been used to characterize materials in preparation for flight, to determine thermal expansion values, and to examine long duration space grown materials, i.e. meteorites. The work will first describe the establishment of the protocol for obtaining the optimum density readings for any material. This will include both the effects of the hardware or instrumental parameters that can be controlled, and the techniques used to process the CT data. Examples will be given of the compositional variation along single crystals of germanium-silicon alloys. Density variation with temperature has been measured in preparation for future materials science experiments; this involved the fabrication and installation of a single zone furnace incorporating a heat pipe to ensure of high temperature uniformity. At the time of writing the thermal expansion of lead has been measured from room temperature to 900 C. Three methods are available. Digital radiography enable length changes to be determined. Prior to melting the sample is small than the container and the diameter change can be measured. Most critical, however, is the density change in solid, through the melting region, and in the liquid state. These data are needed for engineering purposes to aid in the design of containment cartridges, and for enabling fluid flow calculations. A second sample, with the lead alloyed with antimony is ready for scanning. This corresponds to the planned composition of Dr. Poirier's flight experiment. Finally, three-dimensional results will be shown of the structure of a two-phase metallic meteorite of metal and sulfide, in which the iron-nickel phase has coarsened during slow cooling over hundreds of millions of years.

Engel, H. Peter↗

Mars 2005 Sample Return Workshop

Convened at the request of Dr. Jurgen Rahe of the NASA Office of Space Science, the purpose of this workshop was to reexamine the science issues that will determine how an optimum sample return mission would be carried out in 2005 given the new context that has emerged for Mars exploration since the last such workshop was held (in 1987). The results and summary of discussion that took place at the meeting are contained in this volume. The community was invited to participate in the preparation of the final written report by browsing through the agenda and reading the text and viewgraphs provided by workshop participants and submitting comments for that section.

Gulick, V. C.↗

Microchemical and Structural Evidence for Space Weathering in Soils from Asteroid Itokawa

The chemistry, microstructure and optical properties of grains on the surfaces of airless bodies are continu-ously modified due to their interactions predominantly with solar energetic ions and micrometeorite impacts. Collectively known as space weathering, this phenomenon results in a discrepancy between remotely sensed spectra from asteroids and those ac-quired directly from meteorites. The return of pristine samples from the asteroid Itokawa provides insight into surface processes on airless bodies and will help in correlating remote sensing data with laboratory analysis of meteorites. Samples and Methods: We examined Itokawa samples RA-QD02-0042-01 and RA-QD-02-0042-02, ultramicrotomed sec-tions of a singular grain prepared by the Hayabusa sample cura-tion team. We analyzed these slices using a 200 keV JEOL 2010F transmission electron microscope (TEM) at Arizona State Uni-versity and a 200 keV JEOL 2500SE TEM at NASA JSC. Both field emission TEMs are equipped with energy-dispersive X-ray spectrometers (EDS) and scanning TEM (STEM) detectors. Results and Discussion: TEM observations reveal that the sectioned grain predominantly consists of a single crystal of low-Ca orthopyroxene, with subsidiary smaller regions of olivine, Fe-Ni sulfide, and Fe-Ni metal. EDS-spectrum imaging and high-resolution TEM (HRTEM) show local, nanocrystalline regions of the outermost 2 to 5 nm of the pyroxene are composed of an Fe-Mg-S-rich and Si- and O-depleted layer that is underlain by a 2- to 5-nm thick amorphous zone enriched in Si. These layers occur in multiple microtome slices and have uniform thicknesses. We also observe localized 'islands' of material on the surface of the pyroxene which HRTEM imaging indicates are amorphous and EDS measurements show are compositionally heterogeneous. A 10- to 60-nm thick partially amorphous zone occurs below the compositionally distinct rim. While this this zone is associated with the compositionally heterogeneous outer layer, it also occurs as a local stand-alone feature on the exterior rim of the grain. Ar-eas of the pyroxene grain rim also exhibit a vesicular texture. The TEM data indicate a complex history of space weather-ing for samples RA-QD02-0042-01 and -02. The outermost layer of nanocrystalline material with varied composition is consistent with previously suggested [3-4] chemical and structural pro-cessing by solar wind ions, with a possible additional role for im-pact vapor deposition [3-4]. The amorphous and compositionally distinct islands on the surface of this grain, similar to lunar glasses, suggest formation through vapor deposition via micrometeor-ite impact events. In comparison, the amorphization and vesicula-tion textures are likely a product of radiation damage from the solar wind. The depth and degree of amorphization, in conjunction with model calculations, will help provide an upper limit on exposure time for these particles.

Thompson, M. S.↗

Uses of Computed Tomography for Characterizing Materials Grown Terrestrially and in Microgravity

Tomography (CT) has proved to be of inestimable use in providing a rapid evaluation of a variety of samples from Mechanics of Granular Materials (MGM) to electronic materials (Ge-Si alloys) to space grown materials such as meteorites. The system at Kennedy Space Center (KSC), because of its convenient geographical location, is ideal for examining samples before launch and immediately after returning to Earth. It also has the advantage of the choice of fluxes, and in particular the use of a radioactive cobalt source, which is basically monochromatic. This permits a reasonable measurement of density to be made from which chemical composition can be determined. Due to the current dearth of long duration space grown materials, the CT instrument has been used: (1) to characterize materials in preparation for flight, (2) to determine thermal expansion values, and (3) to examine long duration space grown materials, i.e. meteorites. This work will first describe the establishment of the protocol for obtaining the optimum density readings for any material. Included will be the effects of the hardware or instrumental parameters that can be controlled, and the techniques used to process the CT data. Examples will be given of the compositional variation along a single crystal of Ge-Si alloy. Density variation with temperature has been measured in preparation for future materials science experiments; this involved the fabrication at MSFC and installation of a single zone furnace at KSC incorporating a heat pipe to ensure high temperature uniformity. At the time of writing the thermal expansion of lead (Pb) has been measured from room temperature to 900 C. Three methods are available. Digital radiography enables length changes to be determined. Prior to melting the sample is smaller than the container and the diameter change can be measured. Most critical, however, is the density change in solid, through the melting region, and in the liquid state. These data are needed for engineering purposes to aid in the design of containment cartridges, and for enabling fluid flow calculations. A second sample, with the Pb alloyed with Sb is ready for scanning. This corresponds to the planned composition of Dr. Poirier s flight experiment. Other materials pertinent to NASA programs such as Al-Cu (Trivedi), CdTe (Banish), HgCdTe (Lehoczky) will be examined below and above the melting point. Finally, three-dimensional results will be shown of the structure of a two-phase metallic meteorite of metal and sulfide, in which the Fe-Ni phase has coarsened during slow cooling over hundreds of millions of years.

Source record↗

Lunar Transport Vehicle Ocupant Protection Requirements

INTRODUCTION The National Aeronautics and Space Administration (NASA) is preparing for future Artemis missions that will return humans to the lunar surface. A critical piece of these future missions is the Lunar Transport Vehicle (LTV), a transportation device to be used on the lunar surface. A vehicular method of transportation will allow for longer duration missions with increased capabilities to conduct research and transport soil, geological samples, or other materials of interest. The objective of this project is to provide occupant protection guidance for the design of the LTV. METHODS At the time of writing of occupant protection requirements, much of the LTV design is unknown. However, several general assumptions have been established. It is assumed that the LTV will be an unpressurized crewed rover with the occupant(s) wearing an EVA suit. Additionally, the occupant must be protected using established health limits as governing criteria to protect the occupant from hazardous levels of acceleration, jerk, and vibration. In addition to the health limits, it is expected that the LTV shall provide a comfortable transportation solution to Lunar operations. Several restraint configurations have been considered to allow for seated and standing operation of the vehicle. RESULTS Preliminary recommendations and metrics were put forward by a cross functional working group with members from Johnson Space Center and Ames Research Center. Exposure to acceleration, vibration, and jerk metrics above the proposed limits could create a risk of occupant injury. In addition to satisfying crew health needs, these limits permit the occupant to meet performance objectives including operating the vehicle and reading displays while traversing the Lunar surface. Initial guidance was refined during a Technical Interchange Meeting with external subject matter experts in industry, academia, and United States Air Force. CONCLUSION Operating a crewed vehicle in a lunar environment is a novel problem with few analogs to provide data. Based on terrestrial data from ISO & MIL Standards, occupant protection recommendations have been identified with several assumptions made about reduced gravity and deconditioned occupants. If the design of the vehicle attenuates energetics to levels below those put forward, there is high confidence the occupant will be able to repetitively operate the vehicle without physical harm or operational issues.

K Dolick↗

Lunar Transport Vehicle Occupant Protection Requirements

The National Aeronautics and Space Administration (NASA) is preparing for future Artemis missions that will return humans to the lunar surface. A critical piece of these future missions is the Lunar Transport Vehicle (LTV), a transportation device to be used on the lunar surface. A vehicular method of transportation will allow for longer duration missions with increased capabilities to conduct research and transport soil, geological samples, or other materials of interest. Multiple missions to the lunar surface throughout the Apollo program also deployed the use of a lunar vehicle, known as the Lunar Roving Vehicle (LRV). The LRV was used during three of the six Apollo missions that successfully landed humans on the moon. Unfortunately, the LRV did not have any onboard instrumentation, and a large portion of the lunar performance evaluation relied on photographic documentation and crew reports. Therefore, we do not know the actual accelerations and vibrations experienced by the crew when driving on the lunar surface. The objective of this document is to provide occupant protection guidance when designing the LTV. Acceleration, vibration, and jerk metrics imparted by the vehicle relative to the occupant shall not exceed those listed in this document. At the time of writing of these requirements, much of the LTV design is unknown. This document details requirements based on multiple possible restraint configurations and crew orientations.

Kevin R. Dolick↗

Use of Computed Tomography for Characterizing Materials Grown Terrestrially and in Microgravity

Computed Tomography (CT) has advanced considerably since being responsible for such dramatic advances in diagnostics within the medical field. It has become a major tool in non destructive evaluation (NDE), and is used in many fields as diverse as coal-mining to metal solidification to examination of rock cores. A review of industrial applications has been written by Dennis. It is only recently that the technique has been seriously used to determine composition through precise measurement of density. While such applications are restricted to cases when there is no ambiguity in the relationship of composition to density. Thus alloy solid solutions lend themselves to compositional analysis provided there is a large change in density with composition. The technique is most useful when rapid non-destructive evaluation is needed. Such cases will occur with samples returning from the International Space Station (ISS) when knowledge of the results could affect future strategies for processing of on-board samples. Experiments from those Principal Investigators (PI) that are most likely to benefit from early CT scanning are discussed. With a dearth of samples, the major emphasis in the first part of this project has been on preparing suitable standards, optimizing the CT technique for these applications, and using the CT system to determine density variations with temperature. An interesting application of CT has been in the examination of meteorites, which can be classified as space-grown materials and will certainly have solidified in a low gravity environment.

Gillies, Donald C.↗

Connecting Returned Apollo Soils and Remote Sensing: Application to the Diviner Lunar Radiometer

The Diviner Lunar Radiometer, onboard NASA's Lunar Reconnaissance Orbiter, has produced the first global, high resolution, thermal infrared observations of an airless body. The Moon, which is the most accessible member of this most abundant class of solar system objects, is also the only body for which we have extraterrestrial samples with known spatial context, returned Apollo samples. Here we present the results of a comprehensive study to reproduce an accurate simulated lunar environment, evaluate the most appropriate sample and measurement conditions, collect thermal infrared spectra of a representative suite of Apollo soils, and correlate them with Diviner observations of the lunar surface. It has been established previously that thermal infrared spectra measured in simulated lunar environment (SLE) are significantly altered from spectra measured under terrestrial or martian conditions. The data presented here were collected at the University of Oxford Simulated Lunar Environment Chamber (SLEC). In SLEC, we simulate the lunar environment by: (1) pumping the chamber to vacuum pressures (less than 10‐4 mbar) sufficient to simulate lunar heat transport processes within the sample, (2) cooling the chamber with liquid nitrogen to simulate radiation to the cold space environment, and (3) heating the samples with heaters and lamp to set‐up thermal gradients similar to those experienced in the upper hundreds of microns of the lunar surface. We then conducted a comprehensive suite of experiments using different sample preparation and heating conditions on Apollo soils 15071 (maria) and 67701 (highland) and compared the results to Diviner noontime data to select the optimal experimental conditions. This study includes thermal infrared SLE measurements of 10084 (A11 - LM), 12001 (A12 - LM), 14259 (A14 - LM), 15071 (A15 - S1), 15601 (A15 - S9a), 61141 (A16 - S1), 66031 (A16 - S6), 67701 (A16 - S11), and 70181 (A17 - LM). The Diviner dataset includes all six Apollo sites at approximately 200 m spatial resolution We find that analyses of Diviner observations of individual sampling stations and SLE measurements returned Apollo soils show good agreement, while comparisons to thermal infrared reflectance under ambient conditions do not agree well, which underscores the need for SLE measurements and validates the Diviner compositional measurement technique.

Greenhagen, B. T.↗

A spaceflight study of synaptic plasticity in adult rat vestibular maculas

Behavioral signs of vestibular perturbation in altered gravity have not been well correlated with structural modifications in neurovestibular centers. This ultrastructural research investigated synaptic plasticity in hair cells of adult rat utricular maculas exposed to microgravity for nine days on a space shuttle. The hypothesis was that synaptic plasticity would be more evident in type II hair cells because they are part of a distributed modifying macular circuitry. All rats were shared with other investigators and were subjected to treatments unrelated to this experiment. Maculas were obtained from flight and control rats after shuttle return (R + 0) and nine days post-flight (R + 9). R + 9 rats had chromodacryorrhea, a sign of acute stress. Tissues were prepared for ultrastructural study by conventional methods. Ribbon synapses were counted in fifty serial sections from medial utricular macular regions of three rats of each flight and control group. Counts in fifty additional consecutive sections from one sample in each group established method reliability. All synapses were photographed and located to specific cells on mosaics of entire sections. Pooled data were analyzed statistically. Flown rats showed abnormal posture and movement at R + 0. They had statistically significant increases in total ribbon synapses and in sphere-like ribbons in both kinds of hair cells; in type II cells, pairs of synapses nearly doubled and clusters of 3 to 6 synapses increased twelve-fold. At R + 9, behavioral signs were normal. However, synapse counts remained high in both kinds of hair cells of flight maculas and were elevated in control type II cells. Only counts in type I cells showed statistically significant differences at R + 9. High synaptic counts at R + 9 may have resulted from stress due to experimental treatments. The results nevertheless demonstrate that adult maculas retain the potential for synaptic plasticity. Type II cells exhibited more synaptic plasticity, but space flight induced synaptic plasticity in type I cells.

Weightlessness↗

The OSIRIS-Rex Asteroid Sample Return: Mission Operations Design

The OSIRIS-REx mission employs a methodical, phased approach to ensure success in meeting the missions science requirements. OSIRIS-REx launches in September 2016, with a backup launch period occurring one year later. Sampling occurs in 2019. The departure burn from Bennu occurs in March 2021. On September 24, 2023, the SRC lands at the Utah Test and Training Range (UTTR). Stardust heritage procedures are followed to transport the SRC to Johnson Space Center, where the samples are removed and delivered to the OSIRIS-REx curation facility. After a six-month preliminary examination period the mission will produce a catalog of the returned sample, allowing the worldwide community to request samples for detailed analysis.Traveling and returning a sample from an Asteroid that has not been explored before requires unique operations consideration. The Design Reference Mission (DRM) ties together space craft, instrument and operations scenarios. The project implemented lessons learned from other small body missions: APLNEAR, JPLDAWN and ESARosetta. The key lesson learned was expected the unexpected and implement planning tools early in the lifecycle. In preparation to PDR, the project changed the asteroid arrival date, to arrive one year earlier and provided additional time margin. STK is used for Mission Design and STKScheduler for instrument coverage analysis.

Astromaterials Acquisition and Curation Office (KT) Overview

The Astromaterials Acquisition and Curation Office has the unique responsibility to curate NASA's extraterrestrial samples - from past and forthcoming missions - into the indefinite future. Currently, curation includes documentation, preservation, physical security, preparation, and distribution of samples from the Moon, asteroids, comets, the solar wind, and the planet Mars. Each of these sample sets has a unique history and comes from a unique environment. The curation laboratories and procedures developed over 40 years have proven both necessary and sufficient to serve the evolving needs of a worldwide research community. A new generation of sample return missions to destinations across the solar system is being planned and proposed. The curators are developing the tools and techniques to meet the challenges of these new samples. Extraterrestrial samples pose unique curation requirements. These samples were formed and exist under conditions strikingly different from those on the Earth's surface. Terrestrial contamination would destroy much of the scientific significance of extraterrestrial materials. To preserve the research value of these precious samples, contamination must be minimized, understood, and documented. In addition, the samples must be preserved - as far as possible - from physical and chemical alteration. The elaborate curation facilities at JSC were designed and constructed, and have been operated for many years, to keep sample contamination and alteration to a minimum. Currently, JSC curates seven collections of extraterrestrial samples: (a)) Lunar rocks and soils collected by the Apollo astronauts, (b) Meteorites collected on dedicated expeditions to Antarctica, (c) Cosmic dust collected by high-altitude NASA aircraft,t (d) Solar wind atoms collected by the Genesis spacecraft, (e) Comet particles collected by the Stardust spacecraft, (f) Interstellar dust particles collected by the Stardust spacecraft, and (g) Asteroid soil particles collected by the Japan Aerospace Exploration Agency (JAXA) Hayabusa spacecraft Each of these sample sets has a unique history and comes from a unique environment. We have developed specialized laboratories and practices over many years to preserve and protect the samples, not only for current research but for studies that may be carried out in the indefinite future.

Allen, Carlton↗

Inhalation Toxicity of Ground Lunar Dust Prepared from Apollo-14 Soil

Within the decade one or more space-faring nations intend to return humans to the moon for more in depth exploration of the lunar surface and subsurface than was conducted during the Apollo days. The lunar surface is blanketed with fine dust, much of it in the respirable size range (<10 micron). Eventually, there is likely to be a habitable base and rovers available to reach distant targets for sample acquisition. Despite designs that could minimize the entry of dust into habitats and rovers, it is reasonable to expect lunar dust to pollute both as operations progress. Apollo astronauts were exposed briefly to dust at nuisance levels, but stays of up to 6 months on the lunar surface are envisioned. Will repeated episodic exposures to lunar dust present a health hazard to those engaged in lunar exploration? Using rats exposed to lunar dust by nose-only inhalation, we set out to investigate that question.

James, John T.↗

Preliminary Examination Process of Apollo Core 73002 - Insights and Lessons Learned From ANGSA for Future Sample Return Missions

Apollo Sample 73002 is part of a 2-foot long “drive tube” (73001/73002) of regolith that was collected from a landslide deposit near Lara Crater at the Apollo 17 site, Station 3. The double drive tube is believed to have penetrated a lunar landslide deposit that was transported from the slope of the South Massif into the TLV [1]. As part of the ANGSA (Apollo Next Generation Sample Analyses) initiative, preparing preliminary examination (PE) catalog of 73002 is a crucial first step for the early identification of material types such as rock fragments, and potential stratigraphy within the core. PE of Apollo core 73002 is distinct from science activities with the main goal to produces a sample catalog with a level of detail about sample characterization that is sufficient for the ANGSA PIs (and later on the lunar sample community) to select and request the samples to conduct their individual, scientific studies. Ultimately, the PE catalog of 73002 will help to establish a better understanding of the stratigraphy of the land slide deposit; the processes of the landslide including the trigger(s) and possibly number of landslide events, as well as the role of volatiles [1] and will aid in the careful preservation of the material for future studies [2].

Apollo↗

Rationale for Mars Rover/Sampler Return mission

A Mars Rover/Sample Return (MRSR) mission is currently being studied for the late 1990's. The objectives of the mission are to better understand the origin and evolution of Mars, to search for evidence of former life, and to improve the knowledge of the Martian environment in preparation for subsequent human exploration. Having formed in a different part of the Solar System from Earth, Mars will provide clues that will better enable the discrimination between conflicting theories of Solar System formations. Mars is also a natural laboratory on which a wide range of geologic and meteorological processes have operated under conditions very different from those on Earth. Samples are needed so that the full range of analytical techniques available here on Earth can be applied to the study of these issues. The rover provides the mobility needed to access different materials, and can be equipped with an analytical capability so that the planet can be sampled intelligently. The rover will also provide the means of exploring the planet on a human scale and performing a wide range of in situ measurements at different locations. Different mission scenarios are currently being studied with the goal of achieveing sample return before the end of the century.

Carr, Michael H.↗

Clean and Cold Sample Curation

Curation of Mars samples includes both samples that are returned to Earth, and samples that are collected, examined, and archived on Mars. Both kinds of curation operations will require careful planning to ensure that the samples are not contaminated by the instruments that are used to collect and contain them. In both cases, sample examination and subdivision must take place in an environment that is organically, inorganically, and biologically clean. Some samples will need to be prepared for analysis under ultra-clean or cryogenic conditions. Inorganic and biological cleanliness are achievable separately by cleanroom and biosafety lab techniques. Organic cleanliness to the <50 ng/sq cm level requires material control and sorbent removal - techniques being applied in our Class 10 cleanrooms and sample processing gloveboxes.

Allen, C. C.↗

Culture-Independent Microbial Air Profiling using a Spaceflight-Compatible Nanopore Sequencing Method

Microbial monitoring of spacecraft air is critical toward assessing the efficacy of microbial controls within the environmental control and life support systems to protect both the crew and the vehicle environment. Additionally, understanding of the atmospheric microbial profiles will be essential toward Mars-forward planetary protection planning. Currently, onboard the International Space Station (ISS), the air is monitored on a quarterly basis using an impaction air sampler. With this method, bacterial and fungal cells and spores are pulled onto plates containing growth medium. Following five days of ambient incubation onboard the ISS, the crew compares the growth to density charts and provides the approximations to the ground. Upon return of the culture plates to the Johnson Space Center’s Microbiology Laboratory, the isolates present are identified for crew health risk assessments. As NASA moves beyond the low-Earth orbit of ISS, sample return will be impractical. As such, a near real-time monitoring capability for the assessment of the spacecraft atmosphere is necessary. Significant strides have been made in recent years to utilize a molecular-based method for microbial profiling of ISS surfaces. The developed method is not dependent on microbial culture, thus removing the associated risk to the crew from high microbial levels, the bias toward detecting only the culturable organisms, and eliminates the need for sample return. The work described here details the evaluation of three different air sampling platforms whose product is amenable to downstream molecular processing and nanopore sequencing. The three samplers were compared in terms of mass and power requirements, ease-of -use, and the resulting data. For the two highest ranking samplers, a basic concept of operations was developed to transfer the sample into the already established preparation and sequencing process. Using these concepts of operations, an in-depth comparison of the molecular data generated was compared to the historical culture-based method. Data from both methods detailed similar microbial profiles, while the molecular method detailed microbial identifications that could not be identified through the culture-based method. The developed method will enable the generation of near real-time microbial profiles of the spacecraft atmosphere.

Brandon J Dunbar↗

Insights into Regolith Dynamics from the Irradiation Record Preserved in Hayabusa Samples

The rates of space weathering processes are poorly constrained for asteroid surfaces, with recent estimates ranging over 5 orders of magnitude. The return of the first surface samples from a space-weathered asteroid by the Hayabusa mission and their laboratory analysis provides "ground truth" to anchor the timescales for space weathering. We determine the rates of space weathering on Itokawa by measuring solar flare track densities and the widths of solar wind damaged rims on grains. These measurements are made possible through novel focused ion beam (FIB) sample preparation methods.

Keller, Lindsay P.↗

Finding Interstellar Particle Impacts on Stardust Aluminium Foils: The Safe Handling, Imaging, and Analysis of Samples Containing Femtogram Residues

Impact ionisation detectors on a suite of spacecraft have shown the direction, velocity, flux and mass distribution of smaller ISP entering the Solar System. During the aphelion segments of the Stardust flight, a dedicated collector surface was oriented to intercept ISP of beta = 1, and returned to Earth in January 2006. In this paper we describe the probable appeareance and size of IS particle craters from initial results of experimental impacts and numerical simulation, explain how foils are being prepared and mounted for crater searching by automated acquisition of high magnification electron images (whilst avoiding contamination of the foils) and comment on appropriate analytical techniques for Preliminary Examination (PE).

Kearsley, A. T.↗