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DOE OSTI2021

Powering Post-COVID-19 Resilient Recovery with Green Stimulus

As the coronavirus disease (COVID-19) pandemic spread globally in early 2020, many governments enacted lockdown measures as an initial containment response. These lockdowns, while effective in slowing infection rates, have also had substantial economic consequences. A series of supply and demand shocks, workforce and supply chain disruptions, and other impacts of the pandemic and ensuing lockdowns have upended the livelihoods for much of the world's population. This quick read spotlights how green stimulus plans and other recovery measures can support near-term recovery and enable longer-term power system resilience against future threats. The term “green stimulus” encompasses fiscal measures (i.e., governmental tax and spending actions) that support short-term economic activity that enhances environmental and natural resource quality over a longer term. The deployment of energy efficiency (EE) and renewable energy (RE) technologies, as well as associated initiatives (e.g., grid modernization, electrification of transport, and so on) have been demonstrably effective in providing this kind of short-run economic boost while simultaneously strengthening the resilience of the power sector. Along with green stimulus packages, concurrent action on the regulatory side—for example, the development of green building codes, updating grid interconnection standards, creating programs and marketplaces for demand-side efficiency, and others—can be a force multiplier in ensuring that public spending achieves maximum impact in near-term recovery and long-term power sector transformation

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

DOE OSTI2023

Accident-Mitigation-Based Safety Adequacy Evaluation at MSRs [Slides]

Accident mitigation based safety adequacy evaluation is anticipated to be less costly and time consuming than accident prevention for molten salt reactors due characteristics of the reactor class. The presentation provides an overview of the technical basis for developing accident mitigation-based regulatory guidance for future commercial molten salt reactor nuclear power plants.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2024

Assessment of Technical Information Needs and Considerations for Front-End Activities for Molten Salt Fuel Types

This report details an assessment of the hazards and safety impacts related to front-end operations of molten salt reactors (MSR) as well as an assessment of existing safety guidance. Publicly available information was evaluated to make recommendations to the U.S. Nuclear Regulatory Commission (NRC) for guidance development and information gathering needs. Five different MSR technologies were evaluated along with information from 1960’s about the Atomic Energy Commission programs for the Aircraft Reactor Experiment and Molten Salt Reactor Experiment. Primary areas of evaluation include fuel cycle facilities, transportation and at reactor fuel mixing operations up to reactor startup. In general, there was difficulty synthesizing publicly available information due to the early stage of the MSR development and proprietary information associated with the reactor technology companies analyzed. There are a few specific recommendations from this report. One overarching paradigm shift that the NRC needs to evaluate is the possibility of collocating fuel cycle facilities with operating MSRs. Although there is no specific prohibition to this in the NRC regulations, the collocation of the fuel cycle facilities with the operating MSR presents a novel approach compared to the light water reactor baseline. More guidance and research into thorium operations and regulation is needed. The hazards associated with the stability of stored fuel constituents need to be evaluated. Some reactor fuel cycles use beryllium, and its use and the associated hazards need further analysis. NRC-approved designs for transportation packages for shipping molten salt fuels and factory-built and fueled reactors are not available.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2024

Alaska Hydrogen Opportunities Report

This inaugural report on Alaska's hydrogen energy opportunities introduces key themes for developing a hydrogen economy in Alaska, establishes a baseline understanding of Alaska's infrastructure and resources relevant to hydrogen, and contextualizes this baseline in terms of national and global initiatives focused on hydrogen. This report could be further developed into a hydrogen roadmap or a statewide hydrogen strategy in the future. The driving group behind this report, the Alaska Hydrogen Working Group, was formed in 2022 to address the increasing interest in hydrogen energy opportunities in Alaska. As of December 2023, there were nearly 200 members representing industry, government, academia, communities, tribal entities, and interested citizens from both inside and outside Alaska. Since its inception, the group has met monthly to discuss issues of interest and to share insights and questions. One task is to synthesize Alaska's hydrogen energy opportunities and potential into a report. This task not only reflects grassroots interest but also responds to objectives outlined in Alaska Statewide: Comprehensive Economic Development Strategy 2022-2027 (Alaska Department of Commerce, Community, and Economic Development, 2022), one of which is to develop and implement a hydrogen roadmap for Alaska. The goals of this report are to enhance knowledge and information sharing, spur innovation and investment that could help transform Alaska's energy systems, and identify economic and workforce development opportunities across the state. Following the elements of the hydrogen economy outlined in the U.S. National Clean Hydrogen Strategy and Roadmap, published in June 2023, this report explores the production, storage/delivery, and end-use options in evolving markets for Alaska, both within and outside the state. It also outlines key opportunities to identify and develop infrastructure investments, policy and regulatory frameworks, future research and demonstration priorities, economic and investment considerations, and workforce programs. These goals and opportunities are intended to support a statewide hydrogen energy economy that is equitable, diverse, environmentally just, and supportive of Indigenous rights and value systems.

08 HYDROGEN↗

DOE OSTI2022

Editorial: Nanotechnology for natural products

Humans around the globe have lived through an unprecedented time. The emergence and rapid spread of coronavirus disease 2019 (COVID-19) have resulted in the loss of millions of lives, and this number is still climbing every day and hour. As a consequence, there have been drastic changes over the past two years in how we live, communicate, and work, some of which may have changed forever: remote conferencing has become a new norm for businesses, schools, and academia; work-from-home and hybrid working is now more than just acceptable in many industries; most importantly, COVID-19 has raised global awareness of infectious diseases and pandemic responses to an exceptional level. Certainly, COVID-19 is not the only disease that is causing human suffering at this moment. Other highly contagious and lethal viruses, such as the human immunodeficiency virus (HIV), Ebola, and the recent addition to the list, Monkeypox, pose extraordinary challenges to modern civilizations. On the other hand, non-communicable diseases, such as heart disease, stroke, and cancer, are still claiming the majority of deaths on a global level (World Health Organization, 2020). Before the availability of synthetic drugs, civilizations worldwide relied on natural products extracted from the environment and various organisms for healthcare. Natural products and their derivatives continue to be critical components in the drug library available today. Indeed, almost a quarter of the new drugs approved by the US Food and Drug Administration (FDA) since 1981 are natural products, botanical mixtures, or their derivatives (Newman and Cragg, 2020). Recent developments in analytical characterization, genome mining, and engineering have further increased interest in drug discovery from the pool of natural products (Atanasov et al., 2021). This Research Topic celebrates a collection of studies at the intersection of natural product research and nanotechnological advances. As a successor and extension of the Research Topic in “Nanotechnology in Traditional Medicines and Natural Products” published last year (Zhang et al., 2021), the present collection focused more on recent developments in employing the synthesis and characterization of nanostructures for the delivery of functional natural products. Specifically, the use of carrier nanostructures to conjugate or encapsulate therapeutically active natural products with carrier nanostructures could enable their robust, targeted delivery tailored to either tackle a specific disease or unlock a drug administration pathway. Over the last decade, nanostructures such as liposomes (Pattni et al., 2015) and lipid nanoparticles (Hou et al., 2021) have been developed as versatile platforms for the inclusion and delivery of a wide range of therapeutical compounds; a significant advance in this aspect is the stabilization of mRNA strands for COVID-19 vaccination recently produced by Pfizer/BioNTech and Moderna (Tenchov et al., 2021). Such engineered nanomaterials are now widely used in the development of new drugs, while regulatory forces, such as the US FDA, remain cautious with the evaluation of each product on a case-by-case basis (Wang and Grainger, 2022). In this collection, Zeng et al. provided a timely review of natural products conjugated to and contained within nanomaterials for the ongoing COVID-19 pandemic. Natural products that are potentially useful in the mitigation of this disease, such as chloroquine and curcumin, supported in nanocarrier systems, have been discussed in detail. More broadly, Cheng et al. comprehensively reviewed the use of liposomes as a nanocarrier for natural products. This article provides a summary of natural product compounds and extracts used to form liposomal structures. Readers can further enjoy the practical aspects of these engineered drugs for the treatment of specific diseases as well as insights into future directions in this field.

peptides↗

DOE OSTI2025

Gene editing to enhance biotic stress tolerance in sugarcane

Sugarcane (Saccharum spp.) contributes approximately 80% of global sugar production and 40% of biofuel while serving as a promising feedstock for bioproducts (Brant et al., 2025). However, productivity faces mounting challenges from biotic threats including fungal, viral and bacterial diseases (Rott, 2018). Traditional breeding approaches are severely constrained by sugarcane’s complex polyploid genome containing 10–12 copies of "hom(oe)ologous genes within an approximately 10 Gb genome (Healey et al., 2024), extending breeding cycles to 12–15 years. Genome editing has revolutionized crop improvement by enabling targeted modifications without necessarily introducing foreign DNA, potentially circumventing regulatory hurdles while accelerating variety development (Li et al., 2022a). For sugarcane, these technologies offer unprecedented opportunities to enhance biotic stress resilience while improving yield and quality. This article examines current progress and prospects for developing biotic stress-tolerant sugarcane through gene editing, emphasizing technical advances, promising gene targets, and strategic approaches for trait stacking.

CRISPR-Cas systems↗

DOE OSTI2024

Defining Detection Limits for Continuous Monitoring Systems for Methane Emissions at Oil and Gas Facilities

Networks of fixed-point continuous monitoring systems are becoming widely used in the detection and quantification of methane emissions from oil and gas facilities in the United States. Regulatory agencies and operators are developing performance metrics for these systems, such as minimum detection limits. Performance characteristics, such as minimum detection limits, would ideally be expressed in emission rate units; however, performance parameters such as detection limits for a continuous monitoring system (CMS) will depend on meteorological conditions, the characteristics of emissions at the site where the CMS is deployed, the positioning of CMS devices in relation to the emission sources, and the amount of time allowed for the CMS to detect an emission source. This means that certifying the performance of a CMS will require test protocols with well-defined emission rates and durations; initial protocols are now being used in field tests. Field testing results will vary, however, depending on meteorological conditions and the time allowed for detection. This work demonstrates methods for evaluating CMS performance characteristics using dispersion modeling and defines an approach for normalizing test results to standard meteorological conditions using dispersion modeling.

Chen, Qining (ORCID:0000000316908091)↗

DOE OSTI2022

Flexible Plant Operation and Generation Technical Program Plan for FY2023

This report presents the Technical Program Plan for Fiscal Years 2023-2027 (FY2023 to FY2027) for the U.S. Department of Energy (DOE) Light Water Reactor Sustainability Program—Flexible Plant Operation and Generation Research Pathway. The objective of this pathway is to carry out the research needed to help nuclear power plants diversify revenue generation for the life of these plants. The purpose of these research and development activities is two fold: (1) to reduce the technical and economic risks of implementing FPOG applications and (2) to provide guidance on relevant safety and environmental operating license reviews, amendments, and renewals. This pathway provides a clear understanding of the benefits of nuclear energy beyond electricity markets. A detailed description of the research and development activities that have been completed and that are planned for FY2023—FY2027 are presented in this report. These activities include completing the development of analysis tools to perform technical and economic assessments of realistic market opportunities for producing secondary energy products near nuclear power plants. They also include developing and demonstrating engineering systems and control concepts to dispatch thermal and electrical power to an industrial user. Additionally, this plan includes developing guidance for addressing potential regulatory and licensing requirements. In addition, the formation, purpose, and activities of a group referred to as the Hydrogen Regulatory Research and Review Group is discussed. An overview is also provided on the potential benefits of the Infrastructure Investment and Jobs Act (IIJA) Bill that will support the commencement of Regional Clean Hydrogen Hubs, and the Inflation Reduction Act (IRA) that provides compelling production tax credits for nuclear electricity and clean hydrogen using nuclear energy.

08 HYDROGEN↗

DOE OSTI2023

Blockchain cybersecurity audit platform

A cybersecurity distributed ledger is provided herein for managing, tracking, auditing, and securing assets in a power infrastructure. The cybersecurity distributed ledger may include a blockchain, and may combine with smart contract or smart negotiation technology, such as in a permissioned proof of authority blockchain. The cybersecurity distributed ledger may manage the complete life cycle of a grid asset, from asset requirement and specification, through production, testing, deployment, maintenance, and retirement. The cybersecurity distributed ledger may create an immutable record of the grid asset, which may be audited for regulatory compliance or build or development compliance. Further, the cybersecurity distributed ledger may store unique identifying information for a grid asset, which may be used to detect a security breach or other tampering with a grid asset. The cybersecurity distributed ledger may also track control or ownership of the grid asset, as well as changes or updates to the grid asset.

Mylrea, Michael E.↗

DOE OSTI2023

Complete Evaluation of ION Cost Reduction Opportunities for LWRS Pathways

The Light Water Reactor Sustainability (LWRS) Program, sponsored by the U.S. Department of Energy (DOE), plays a pivotal role in enhancing the sustainability, safety, and economic viability of the nation's fleet of nuclear power plants. This program collaborates extensively with industry, vendors, suppliers, regulatory agencies, and research and development organizations within the nuclear energy sector. The overarching goals of the LWRS program are twofold: to provide innovative science and technology-based solutions to the nuclear industry, surpassing current performance models, and to manage the aging of systems, structures, and components (SSCs) to extend the operational lifetimes of nuclear power plants safely, efficiently, and economically. The program focuses on five research and development pathways: Plant Modernization: This pathway seeks to enhance the economic viability of nuclear power plants in evolving energy markets through innovation, efficiency improvements, and digital transformation. The integration of digital technologies into plant operations is a central theme, aiming to create a seamless digital environment that improves plant economics and safety. Flexible Plant Operation and Generation: Research efforts in this pathway explore opportunities for light-water reactors (LWRs) to directly supply energy to industrial processes, diversifying revenue generation approaches and improving economic sustainability.

42 ENGINEERING↗

DOE OSTI2020

Reducing Cost of Chlorinated Volatile Organic Compound Remediation by Transitioning from Active to Passive Soil Vapor Extraction - 20157

Areas of high chlorinated volatile organic compound (cVOC) contamination at the Savannah River Site (SRS) have been undergoing remediation via soil vapor extraction, sometimes coupled with thermal treatments to enhance extraction rates. These active systems are effective in removing large amounts of contaminant mass from the subsurface and mitigating the impacts to groundwater. However, as extraction rates decline, costs must be evaluated with respect to the benefit of continued active operation. A decision framework for identifying conditions when a transition to a more passive remediation is appropriate has been developed with state and federal regulatory agencies. Two remediation areas have recently been transitioned from active soil vapor extraction (ASVE) to passive soil vapor extraction (PSVE) at the SRS. Performance evaluation goals including plume stabilization, overall mass removal trends, environmental sustainability and costs were considered in transitioning from active remediation to passive technologies at both sites. At the Dynamic Underground Stripping (DUS) project at the M-Area Settling Basin, ASVE was combined with steam injection to extract cVOCs during active operations. Steam injection occurred from September 2005 to September 2009. DUS utilized 63 steam injection wells, 34 active vapor extraction wells, and 3 active soil vapor extraction units (SVEUs). Two active SVEUs had 60 horsepower blowers; the third had a 25-horsepower blower. Mass removal was closely tracked during DUS operations; over 181,437 kilograms (400,000 pounds) of cVOCs were removed while active steam injection occurred. After steaming was stopped, ASVE continued. The 34 active wells were evaluated in 2012. The ASVE wells were grouped into categories of high, medium, and low extraction rates. High producing wells remained connected to a single active SVEU. Low producing wells were abandoned, and the medium producing wells were transitioned to PSVE (Microblowers{sup TM}). Microblowers{sup TM} utilize a dedicated blower per well and are solar powered. This passive technology provides energy, maintenance, and operation costs savings while still providing an efficient reduction in cVOC migration to groundwater. In 2018, the remaining ASVE wells were evaluated again. The purpose of this testing was to identify which wells removed the most mass. An optimal well configuration was determined. The criteria to discontinue ASVE was removal of less than 18 kilograms (40 pounds) per week of cVOCs. After 3 months of shutdown (rebound conditions), 2.7 kilograms (5.9 pounds) of cVOCs per week were being removed. Data from the rebound test justified ending ASVE and transitioning wells with higher extraction rates to PSVE. Wells that had depleted the cVOC mass within their zone of influence were abandoned. Performance data from existing PSVE wells justified ending PSVE at wells with depleted extraction rates. Currently the system has 16 PSVE wells operating. Another ASVE system was being used to treat cVOC contaminated soil at the A-Area Miscellaneous Rubble Pile (AMRP) at SRS. System operation began in 2004, with 7 ASVE wells connected to a 60- horsepower blower. Mass removal rates and contaminant concentrations remained consistently low over the ASVE lifespan at AMRP. This indicated that mass removal was diffusion limited. With this data, the 7 ASVE wells were transitioned to PSVE in 2017. Twelve pressure monitoring points were also transitioned to PSVE wells. AMRP currently has 19 PSVE wells operating. Both transitions from active to passive remediation had concurrence from the United States Environmental Protection Agency and the South Carolina Department of Health and Environmental Control. These transitions ensure that only the necessary amount of energy is being exerted to remediate the environment. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2024

The NREL Sensor Laboratory: Hydrogen Leak Detection for Large Scale Deployments: Preprint

The NREL Hydrogen Sensor Laboratory was commissioned in 2010 as a resource for sensor developers, end-users, and regulatory agencies within the national and international hydrogen community. The Laboratory continues to provide as its core capability the unbiased verification of hydrogen sensor performance to assure sensor availability and their proper use. However, the mission and strategy of the NREL Sensor Laboratory has evolved to meet the needs of the growing hydrogen market. The Sensor Laboratory program has expanded to support research in conventional and alternative detection methods as hydrogen use expands to large-scale markets as envisioned by the DOE National Clean Hydrogen Strategy and Roadmap. Current research encompasses advanced methods of hydrogen leak detection including stand-off and wide area monitoring approaches for large scale and distributed applications. In addition to safety applications, low-level detection strategies to support the potential environmental impacts of hydrogen and hydrogen product losses along the value chain are being explored. Many of these applications utilize detection strategies that supplement and may supplant the use of traditional point sensors. The latest results of the hydrogen detection strategy research at NREL will be presented.

detection↗

DOE OSTI2020

Team Dynamics and Development in Chemical Emergency Response

This presentation is focused on Team Dynamics and Development in Chemical Emergency Response. While regulatory requirements focus on defining the performance requirements associated with this category of response team, there is very little information and guidance provided to team leaders on the criticality of team dynamics of development in performance. These elements are essential to achieving successfully integrated team operations. NREL has worked to expand chemical response team participation to include operational personnel from research, support operations, and various technical areas. Since this team expansion has encompassed integration from numerous levels and areas within the organization, a systematic approach was adopted to better understand how organization culture, dynamics, and diversity can be used to be more effective. Benchmarking other organizations and reviewing relevant literature assisted in laying the foundation to develop a strategy that incorporates team development principles, dynamics, and leadership practices to increase the effectiveness of emergency response operations. This presentation focuses on the valuable lessons learned and the team-based approaches that can be used to develop a successful response team.

emergency response↗

DOE OSTI2023

Developing energy flexibility in clusters of buildings: A critical analysis of barriers from planning to operation

This paper examines building energy flexibility at an aggregated level and addresses the main barriers and research gaps for the development of this resource across three design and development phases: market and policy, early planning and design, and operation. We review methodologies and tools and discuss barriers, challenges, and opportunities, incorporating policy, economic, technical, professional, and social perspectives. Although various legal and regulatory frameworks exist to foster the development of energy flexibility for small buildings, financing mechanisms are limited with a significant number of perceived risks undermining private investment. For the early planning and design phase, planners and designers lack appropriate tools and face interoperability challenges, which often results in insufficient consideration of demand response programs. The review of the operational phase highlighted the socio-technical challenges related to both the complexity of deployment and communication, as well as privacy and acceptability issues. Finally, the paper proposes a number of targeted research directions to address challenges and promote greater energy flexibility deployments, including capturing building demand side dynamics, improving baseline estimations and developing seamless connectivity between buildings and districts.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

DOE OSTI2020

Policy and Regulatory Environment for Utility-Scale Energy Storage: India

This report is part of a series investigating the potential for utility-scale energy storage in South Asia. The first part of the work is focused on opportunities and barriers for energy storage within existing policy and regulatory frameworks in the region. This report applies the previously-developed Energy Storage Readiness Assessment to evaluate the policy and regulatory environment for energy storage in India. Forthcoming reports will expand this analysis to other countries in South Asia. These evaluations are designed to provide insights on the opportunities and barriers for energy storage growth and deployment in each country. The second part of this work consists of techno-economic analysis to understand the drivers of energy storage investments in South Asia. Using NREL’s power system planning and operational models of South Asia, this analysis will identify potential storage applications and growth under various cost, policy, and demand growth scenarios. In addition, the regulatory and policy barriers and incentive mechanisms identified in the first task will be incorporated into the modeling task to understand their impact on energy storage deployment and operation. Together these studies will inform the applications and value of energy storage on South Asia power systems and policy and regulatory pathways to realize this value.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

DOE OSTI2020

Sensitive Resources Assessment and Forest Analysis for the Proposed Versatile Test Reactor, Oak Ridge, Tennessee

The US Department of Energy’s (DOE’s) Oak Ridge National Laboratory (ORNL) is a leading institution in advanced materials, supercomputing, neutrons, and nuclear science. As a research laboratory managed by UT-Battelle, LLC for DOE, ORNL has national priorities in energy, security, and scientific discovery that necessitate facility improvements and expansions. DOE is also committed to environmental stewardship. The laboratory is located on the ~32,000-acre (~13,000-ha) Oak Ridge Reservation (ORR), much of which is categorized as a National Environmental Research Park (NERP) and a state Wildlife Management Area. DOE works with the Tennessee Wildlife Resources Agency (TWRA), Tennessee Department of Environment and Conservation (TDEC), US Fish and Wildlife Service (USFWS), US Department of Agriculture, and other agencies to serve as an effective steward of the ORR. Accordingly, project managers must conform to environmental regulations, agreements, and policies at the federal, state, and institutional levels. Per 40 CFR (Code of Federal Regulations) 1508.14, potential effects on research and science education also represent potential effects of federal actions on the NERP, and impacts on, e.g., deer harvest, must be considered on the Oak Ridge Wildlife Management Area when other aspects of the human environment are affected. The United States currently has no fast neutron testing capability to support advanced nuclear research and development. The proposed Versatile Test Reactor (VTR) will take advantage of current investments by the US government and private industry in nuclear reactors to expedite the design and construction process, using proven technology to create a world-class scientific infrastructure. The VTR will take advantage of fast neutrons provided by this proven technology, along with a capability to rapidly insert, conduct, and remove state-of-the-art experiments. An advantage of the VTR is that it can support future innovations in experimental capabilities without modifying the facility. The VTR will support progress in a variety of science and technology areas, including testing and qualification of advanced reactor fuels; testing and qualification of innovative structural materials; testing of innovative components and instruments; validation of advanced modeling and simulation tools; and versatility for future technical missions. Through proven technology, the VTR can take advantage of existing reactor designs and operating experience to reduce the risk, cost, and time for design and construction. The top available resources of DOE laboratories, industry, and universities will be used to expedite reactor design and construction toward developing the scientific infrastructure that affords a strong testing capability that can be sustained over many years. This report summarizes current knowledge of natural and cultural resources primarily within the VTR construction area. At the time of this report, the proposed VTR site design includes a construction area of ~150.4 acres (~69.9 ha), which contains an ~51.3-acre (~20.8-ha) operations area, located within forested natural areas of the ORR. The primary goal of the work presented here was to evaluate potential effects on sensitive resources that might result from development and construction activities associated with VTR. In addition to on-the-ground surveys during spring and summer 2020 by the ORNL Natural Resources Management Program and Aquatic Ecology Group staff, this report makes use of historical (pre-1995) and contemporary (1995 to present) data from additional confirmed sources (e.g., TDEC). Likewise, forest conditions were compiled from a 2011 forest inventory and supplemented with limited ground observations in 2020. The individuals who obtained and compiled the data presented here are familiar with and routinely assess sensitive resources on the ORR. Anyone who references this report must consider that the timing of surveys did not permit a complete delineation of the resources that will be affected. If the VTR project proceeds, additional surveys will be required to account for the seasonal patterns of various threatened and endangered species. Data deficiencies and potential resources that likely went undetected are indicated where possible. Accordingly, this report should facilitate more environmentally sound decisions during planning and 2 development of the VTR site, provide a foundation for further assessment of sensitive and cultural resources, and help project managers better address regulatory guidance and DOE policies on sustainable development in compliance with, for example, the US Endangered Species Act (ESA), Migratory Bird Treaty Act (MBTA), Tennessee Rare Plant Protection and Conservation Act of 1985, Tennessee Nongame and Endangered or Threatened Wildlife Species Conservation Act of 1974, several federal and state regulations regarding aquatic resource protection, and site-specific policies as outlined in various ORR management plans developed by ORNL and TWRA for DOE.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2026

Cis-regulatory evolution shapes facial diversity in birds and mammals

Birds and mammals exhibit extraordinary facial diversity, reflecting adaptations to distinct ecological niches and feeding strategies. While core face-building developmental programs are conserved and orchestrated by interactions between ectodermal organizers and the underlying mesenchyme, mechanisms driving facial shape variation remain poorly understood. Here, we integrate single-cell transcriptomic and chromatin accessibility profiling of mouse and chicken developing face to construct a comparative regulatory map. Although both ectodermal and mesenchymal populations display distinct regulatory features in each species, the mesenchyme exhibits markedly greater divergence, pointing to its central role in shaping facial morphology. We further reveal unexpected molecular complexity in the main face-shaping organizer, including a mouse-specific Shh/Wnt5a expression domain. At key morphogen loci (Bmp4, Fgf8, and Wnt5a), conserved and lineage-specific enhancers exhibit spatially restricted activity patterns that mirror divergent signaling domains. These findings demonstrate how cis-regulatory evolution modulates conserved developmental programs to generate morphological novelty, providing a valuable resource for studying vertebrate facial evolution.

Kyomen, Stella [Max Planck Institute for Evolution↗

DOE OSTI2025

Multi-Omics Reveals Temporal Scales of Carbon Metabolism in Synechococcus Elongatus PCC 7942 Under Light Disturbance

Central carbon metabolism in model cyanobacteria involves multiple pathways to adapt to energy-light limitations across diel cycles. However, the success in mechanistic modeling for phenotypic prediction of the protein regulators in the metabolic state depends on capturing the vast possibilities emerging from multiple regulatory pathways in complex biological processes. Here, we developed a physics-informed machine learning approach based on energy-landscape concepts to predict regulatory proteins responding to cyclic circadian and unforeseen light perturbations in cyanobacterial metabolic networks. Our approach provides interpretable de novo models for inferring gene expression dynamics from Synechococcus elongatus over diel cycles and using redox proteome analysis to distinguish immediate light-responsive elements from circadian-regulated processes in carbon metabolism pathways. We identified distinct temporal signatures with the analysis of the redox proteome: there was an immediate shift in cysteine redox states accompanied by a limited change in protein abundance under constant illumination and after 2 hours of darkness. This discovery indicates that the generation of reductants coordinates photoinduced electron transport with redox metabolic pathways in two discernable molecular mechanisms: fast redox-based protein modifications occur immediately after the light disturbance, followed by slow transcriptional regulations across networks. This temporal regulation reveals how metabolic networks integrate rapid light responses with programmed circadian rhythms to maintain cellular homeostasis under the light-energy limitations over the diel cycle.

Biomolecular & subcellular processes↗