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At least 181 records · Page 10

Detection of DNA Damage by Space Radiation in Human Fibroblasts Flown on the International Space Station

Although charged particles in space have been detected with radiation detectors on board spacecraft since the discovery of the Van Allen Belts, reports on the effects of direct exposure to space radiation in biological systems have been limited. Measurement of biological effects of space radiation is challenging due to the low dose and low dose rate nature of the radiation environment, and due to the difficulty in distinguishing the radiation effects from microgravity and other space environmental factors. In astronauts, only a few changes, such as increased chromosome aberrations in their lymphocytes and early onset of cataracts, are attributed primarily to their exposure to space radiation. In this study, cultured human fibroblasts were flown on the International Space Station (ISS). Cells were kept at 37 degrees Centigrade in space for 14 days before being fixed for analysis of DNA damages with the gamma-H2AX assay. The 3-dimensional gamma-H2AX foci were captured with a laser confocal microscope. Quantitative analysis revealed several foci that were larger and displayed a track pattern only in the Day 14 flight samples. To confirm that the foci data from the flight study was actually induced from space radiation exposure, cultured human fibroblasts were exposed to low dose rate gamma rays at 37 degrees Centigrade. Cells exposed to chronic gamma rays showed similar foci size distribution in comparison to the non-exposed controls. The cells were also exposed to low- and high-LET (Linear Energy Transfer) protons, and high-LET Fe ions on the ground. Our results suggest that in G1 human fibroblasts under the normal culture condition, only a small fraction of large size foci can be attributed to high-LET radiation in space.

Lu, Tao↗

X-ray observations of pulsars.

The X-ray pulsations from NP 0532, discovered by Fritz et al. (1969), have been studied. The detectors in the investigations had wide fields of view, and the entire Crab Nebula NP 0532 radiation was detected. The power density spectrum of X-radiation observed is presented together with the shapes of X-ray and optical pulses. Searches for X-ray pulsations from other X-ray sources have also been carried out. The summarized results are shown in a table.

Bradt, H. V.↗

Detection of 2.6-millimeter radiation probably due to nitrogen sulfide

Results are reported for observations of millimeter-wavelength radiation in Sgr B2 at two frequencies corresponding to the transitions between the J = 5/2 and J = 3/2 Lambda-doublet sublevels in the 2 Pi(1/2) state of nitrogen sulfide. Difficulties in making a positive identification of the source of this radiation are discussed which result from the apparently anomalous ratio of the hyperfine components. However, it is noted that observations of the d-state by Gottlieb et al. (1975) showing the hyperfine components more exactly in their optically thin ratio confirm that the observed radiation is due to nitrogen sulfide.

Kuiper, T. B. H.↗

Detecting a gravitational-radiation background using spacecraft Doppler tracking

The sensitivity of NASA's Deep Space Network spacecraft tracking system to an isotropic cosmological background of gravitational radiation is analyzed. It is found that by using the autocovariance function of the Doppler record in the so-called 'three-way' tracking mode to dig into the noise it is possible to put significant limits on this background with current and future planned deep-space missions.

Hellings, R. W.↗

Far-Infrared and Millimeter Continuum Studies of K-Giants: Alpha Boo and Alpha Tau

We have imaged two normal, non-coronal, infrared-bright K-giants, alpha Boo and alpha Tau, in the 1.4-millimeter and 2.8-millimeter continuum using BIMA. These stars have been used as important absolute calibrators for several infrared satellites. Our goals are: (1) to probe the structure of their upper photospheres; (2) to establish whether these stars radiate as simple photospheres or possess long-wavelength chromospheres; and (3) to make a connection between millimeter-wave and far-infrared absolute flux calibrations. To accomplish these goals we also present ISO Long Wavelength Spectrometer (LWS) measurements of both these K-giants. The far-infrared and millimeter continuum radiation is produced in the vicinity of the temperature minimum in a Boo and a Tau, offering a direct test of the model photospheres and chromospheres for these two cool giants. We find that current photospheric models predict fluxes in reasonable agreement with those observed for those wavelengths which sample the upper photosphere, namely less than or equal to 170 micrometers in alpha Tau and less than or equal to 125 micrometers in alpha Boo. It is possible that alpha Tau is still radiative as far as 0.9 - 1.4 millimeters. We detect chromospheric radiation from both stars by 2.8 millimeters (by 1.4 millimeters in alpha Boo), and are able to establish useful bounds on the location of the temperature minimum. An attempt to interpret the chromospheric fluxes using the two-component "bifurcation model" proposed by Wiedemann et al. (1994) appears to lead to a significant contradiction.

Cohen, Martin↗

Plasma radiation diagnostics of the primary energy release region in solar flares

The possibility is investigated that the plasma turbulence used in many recent models of the primary energy release and acceleration in solar flares should be detectable by radiation near the fundamental and second harmonic of the plasma frequency. Formulae are derived for fundamental emission due to the combination of ion-acoustic and Langmuir plasma turbulence and for second harmonic emission due to the combination of two Langmuir waves. These results are applied to recent primary energy release and acceleration models which shows that either such radiation should be detectable and possibly distinguishable with suitable microwave interferometers or that its absence places fairly stringent constraints on the possible level of Langmuir or Langmuir and ion-acoustic waves in these models.

Smith, D. F.↗

Detection of DNA Damage by Space Radiation in Human Fibroblast Cells Flown on the International Space Station

Although charged particles in space have been detected with radiation detectors on board spacecraft since the early discovery of the Van Allen Belt, reports on effects of direct exposure to space radiation in biological systems have been limited. Measurement of biological effects of space radiation has been difficult due to the low dose and low dose rate nature of the radiation environment, and the difficulty in separating the radiation effects from microgravity and other space environmental factors. In astronauts, only a small number of changes, such as increased chromosome aberrations in lymphocytes and early onset of cataracts, attributed primarily to the exposure to space radiation. In a recent experiment, human fibroblast cells were flown on the International Space Station (ISS). Cells fixed on Days 3 and 14 after reaching orbit were analyzed for phosphorylation of a histone protein H2AX by immunofluorescent staining of cells, which is a widely used marker for DNA double strand breaks. The 3-dimensional gamma-H2AX foci were captured with a laser confocal microscope. Quantitative analysis revealed a small fraction of foci that were larger and displayed a track pattern in the flight samples in comparison to the ground control. Human fibroblast cells were also exposed to low dose rate gamma rays, as well as to protons and Fe ions. Comparison of the pattern and distribution of the foci after gamma ray and charged particle exposure to our flight results confirmed that the foci found in the flown cells were indeed induced by space radiation.

Wu, Honglu↗

In search of Uranian decametric emission

An experiment to detect decametric radiation from Uranus is described. It is argued that in light of recent discoveries of a magnetic field on Uranus, it may be possible to detect low-frequency radio emission similar to the decametric emission from Jupiter. The experiment consisted of 106 hours of monitoring using the 26.3 MHz array of the University of Florida Dixie County Radio Observatory. No radiation from Uranus was detected. An upper flux density limit of 400 Jy was obtained.

Lebo, G. R.↗

The Impact of Lidar Detection Sensitivity on Assessing Aerosol Direct Radiative Effects

Spaceborne lidar observations have great potential to provide accurate global estimates of the aerosol direct radiative effect (DRE) in both clear and cloudy conditions. However, comparisons between observations from the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite (CALIPSO) and multiple years of Atmospheric Radiation Measurement (ARM) program’s ground-based Raman lidars (RL) show that CALIPSO does not detect all radiatively significant aerosol, i.e. aerosol that directly modifies the Earth’s radiation budget. We estimated that using CALIPSO observations results in an underestimate of the magnitude of the global mean aerosol DRE by up to 54%. The ARM RL datasets along with NASA Langley airborne high spectral resolution lidar (HSRL) data from multiple field campaigns are used to compute the detection sensitivity required to accurately resolve the aerosol DRE. This shows that a lidar with a backscatter coefficient detection sensitivity of about 1−2x10(exp -4)km(exp -1)sr(exp -1) at 532nm would resolve all the aerosol needed to derive the DRE to within 1%.

Tyler J. Thorsen↗

A new thermal radiation detector using optical heterodyne detection of absorbed energy

The operating principles of a new kind of room-temperature thermal radiation detector are described. In this device modulated light heats a gas, either directly or by conduction from a thin absorbing membrane, and the resultant change in density of the gas is detected by optical heterodyning. The performance of a membrane device of this kind agrees well with the predictions of theory.

Davis, C. C.↗

Yeast Strain Development and Hardware Testing in Preparation of a Lunar BioSensor

With Artemis missions underway, it is clear we are going back to the Moon to stay. Before sending Astronauts for long-duration missions, it is crucial to understand the technological and biomedical countermeasures needed to protect them before they get there. We can use knowledge gained from biological CubeSats to guide the next generation of experiments to support human habitation on the Moon. Lunar Explorer Instrument for space biology Applications (LEIA) is NASA’s latest BioSensor, adapted BioSentinel, the only CubeSat to travel Beyond Low Earth Orbit. BioSentinel launched on Artemis I and is currently >50 million kilometers from Earth (as of July 2024). LEIA aims to identify biological responses to the Lunar environment, which unprotected against would pose a threat to astronauts (cancer, cardiovascular disease, neurological impairment). The suite of instruments within LEIA detects Lunar radiation using two on-board radiation sensors (ARES charged particle detector, Mini-Fast Neutron Detector), then monitors real-time biological responses to the Lunar environment via an autonomous microfluidic system, fit with 3-LED emitter and detector boards and the alamarBlue metabolic indicator dye. LEIA will use a genetic approach in addition to synthetic biology to test counter-measure production in space, with the goal to inform and protect astronauts for future Moon missions. We have conducted preliminary tests in preparation for launch to the anticipated South Pole of the Moon, optimizing the biology (strain down-selection, desiccation tolerance, radiation sensitivity) and improving the hardware (including a blue LED to detect the beta-carotene countermeasure product). Our team will discuss these findings in several parts – an overview of the LEIA mission (Mark Settles), adapting flexible CubeSat platforms for deep-space applications (Sergio Santa Maria, Kira Rienecker), developing new technologies to support LEIA ground studies (Chinmayee Govinda Raj), and yeast strain development and hardware testing in preparation for LEIA (presented here).

synthetic biology↗

The pyroelectric properties of TGS for application in infrared detection

The pyroelectric property of triglycine sulfate and its application in the detection of infrared radiation are described. The detectivities of pyroelectric detectors and other types of infrared detectors are compared. The thermal response of a pyroelectric detector element and the resulting electrical response are derived in terms of the material parameters. The noise sources which limit the sensitivity of pyroelectric detectors are described, and the noise equivalent power for each noise source is given as a function of frequency and detector area.

Kroes, R. L.↗