[Data Set] East Fork Poplar Creek Discharge at Kilometer 5.4 Water Year 2019
Water level, discharge and temperature data for East Fork Poplar Creek at creek kilometer 5.4
RIVER DISCHARGE, FRESH WATER RIVER DISCHARGE, ELEV↗
SEARCH · Engineering Papers
Results for “POPLARS”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
[Data Set] East Fork Poplar Creek Sonde Data at Kilometer 5.4 Water Year 2019
This data set reports stream water temperature, specific conductance, pH, dissolved oxygen percent saturation and dissolved oxygen concentration for East Fork Poplar Creek at a location approximately 5.4 kilometers upstream from the mouth of the creek.
East Fork Poplar Creek↗
[Data Set] East Fork Poplar Creek Discharge at Kilometer 16.2 Water Year 2019
This data set reports water temperature, water depth, water surface elevation, and stream discharge for East Fork Poplar Creek at a location approximately 16.2 kilometers upstream from the mouth of the creek.
East Fork Poplar Creek↗
[Data Set] East Fork Poplar Creek Discharge at Kilometer 5.4 Water Year 2022
This data set reports stream discharge and water temperature for East Fork Poplar Creek at a location approximately 5.4 kilometers upstream from the mouth of the creek.
discharge↗
[Data Set] East Fork Poplar Creek Discharge at Kilometer 16.2 Water Year 2022
This data set reports stream discharge and water temperature for East Fork Poplar Creek at a location approximately 5.4 kilometers upstream from the mouth of the creek.
discharge↗
Ultraclean hybrid poplar lignins via liquid–liquid fractionation using ethanol–water solutions
As recovered from the byproducts stream of a cellulosic ethanol biorefinery, the renewable biopolymer lignin is too impure and polydisperse for many proposed applications. By mixing a hybrid poplar lignin with hot ethanol–water solutions, two liquid phases, one polymer-rich and one solvent-rich, are created. This liquid–liquid equilibrium phenomenon was used to generate solvated (and thus liquefied) lignin fractions of controlled molecular weight for which the impurities analyses for sugars and ash were near or below the limits of detection. Furthermore, those carbohydrates and metals impurities end up highly concentrated in a single process stream also having potential value.
09 BIOMASS FUELS↗
Extreme Expression of Cellulases in Poplar
Plant cell walls provide a vast, untapped source of sugars in the form of cellulose that can be fermented for the production of ethanol and other biofuels. However, there are several roadblocks to developing an economically viable cellulosic ethanol production process based on biochemical conversion, one of which is the cost of the enzymes required to change the cellulose to glucose. Historically, cellulase enzymes for digesting plant biomass have been produced and purified using bacteria. An alternative and potentially more economic approach is the production of the enzymes within the plant itself. The goal of this research was to verify a transgenic technology (In Plant Activation Technology – INPACT) in poplar which allows for the controlled high level accumulation of enzymes within the plant (Dugdale et al., 2013), and to assess the impact of the resulting cellulases on the efficiency of converting cellulose to fermentable sugars. INPACT is an inducible, gene amplification system which is unique in two ways. First, the INPACT gene cassette is designed so that the gene of interest is only transcribed in the presence of an initiation protein and as a result, no functional protein expression can occur without the initiation protein. Second, expression of the initiation protein is regulated by an inducible promoter such that INPACT acts as a switch to turn on high-level expression of proteins within the plant, and is particularly suited to proteins that may be toxic or hinder plant development as expression is tightly controlled by the INPACT trigger. The INPACT expression system combines the advantages of both stable and transient expression, and significantly, can be used to produce “difficult” proteins that cannot be expressed within the plant using conventional approaches.
09 BIOMASS FUELS↗
A novel poplar biomass germplasm resource for functional genomics and breeding. Final report addendum
In this report, we report and update activities associated with co-PI Groover. This report is an addendum to the full report detailing genomic objectives associated with the project, submitted by PI Luca Comai and co-PI Isabelle Henry. The Groover lab is leading the efforts to produce and replicate poplar irradiation and control hybrids genotypes, and phenotype the hybrids. These efforts are coordinated with Comai and Henry to ensure the most promising hybrids are included for field and greenhouse-based studies, and to link the genomic and phenotypic analyses.
09 BIOMASS FUELS↗
HSQC spectra of lignin isolated from poplar stems
Here we present a curated dataset of two-dimensional heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectra of lignin isolated from stems of genetically engineered poplar through auxin signaling gene modification. The plants were grown in greenhouse with temperatures between 21 and 23 °C. Plants were harvested and the aboveground stems were cut off an approximately five-inch-long segment from the bottom end of the plant stem, debarked and air-dried for three weeks. The dried stem samples were Wiley milled (mesh size 20), Soxhlet-extracted with toluene/ethanol for 24 h to remove extractives. The extracted biomass was ball-milled in a Retsch PM100 planetary ball mill using a porcelain jar with ceramic balls at 600 rpm for 2 h (in 5 min on and 5 min off cycles to avoid excessive sample heating). The ball-milled materials were then subjected to enzymatic hydrolysis for 48 h followed by centrifugation and washing with deionized water. The solid residue was freeze-dried to recover the lignin. The dry stem lignin samples were dissolved in deuterated dimethyl sulfoxide (d6) and transferred into a 5 mm tube. 13C–1H HSQC experiments were performed in a Bruker Avance III HD 500 MHz NMR spectrometer operating at a frequency of 125.12 MHz for the 13C nucleus using a standard Bruker pulse sequence on a Prodigy platform cryoprobe. The NMR spectra were acquired under the following acquisition conditions: 230 ppm spectral width in F1 (13C) dimension with 256 data points and 12 ppm spectral width in F2 (1H) dimension with 2048 data points, a 90° pulse, a one bond C–H coupling constant of 145 Hz, a 1.0 s pulse delay, and 64 scans. Spectra were processed using the Bruker TopSpin 3.6 software. Additional meta data is embedded in the raw spectra figures.
HSQC, lignin, poplar, stems, CBI↗
Supporting Information for manuscript: “A latitudinal gradient in S/G lignin monomer ratio driven by laccase in natural poplar variants”
Lignin composition plays a crucial role in plant structural integrity and environmental adaptation. However, the genetic and molecular mechanisms underlying natural variation in lignin composition remain poorly understood. This study investigates the syringyl-to-guaiacyl (S/G) lignin monomer ratio across a natural population of Populus trichocarpa spanning a latitudinal gradient along the Northwest coast of North America. By integrating biochemical, genomic, and geographic analysis, we identify key gene variants associated with S/G ratio differences. These datasets provide valuable insights into the evolutionary and functional genomics of lignin composition and serve as a resource for developing poplar variants optimized for forestry and bioenergy applications.
Poplar, lignin composition, laccases, latitude, ad↗
23_POPLAR_EXUDATES
This data is derived from sampling poplar trees at the GWAS common garden in Clatskanie, Oregon. This is intermediate level data from LCMS experiments that were done at ORNL from root tissue and soluble root exudates collected from 24 trees in the common garden in October of 2022.
CBI↗
Lignin engineering in poplar via heterologous expression of dehydroshikimate dehydratase induces distinct transcriptional and metabolic changes in the shikimate and phenylpropanoid pathways
Understanding how crops respond to such genetic modifications at the transcriptional and metabolic levels is needed to facilitate further improvement and field deployment. In this work, we gathered some fundamental knowledge on lignin-modified QsuB poplar grown in a greenhouse using RNA-seq and metabolomics.
Aromatics↗
Finding Fingerprints of Selection in Poplar Genomes
Jerry Tuskan of Oak Ridge National Laboratory and the DOE JGI talks about poplar trees as models for selective adaptation to an environment. This video complements a study published ahead online August 24, 2014 in Nature Genetics.
54 ENVIRONMENTAL SCIENCES↗
Phasing Variants in Poplar Trees using a Hybrid of Short & Long Read Technologies
Poplar grow throughout the West coast & are adapted to extremely variable conditions. To examine what allows for this wide range of growth conditions, Jerry Tuskan's team has collected 1000 different individuals from British Columbia to California. In 2009, three Common Gardens were established where each individual was cloned in triplicate. Nearly all of these trees have been sequenced using short read technology, revealing a huge degree of variation in genotype. Correlating this genomic variation to phenotype would be greatly be strengthened if the variants could be phased into long haplotype blocks.
99 GENERAL AND MISCELLANEOUS↗
Recent Approaches to Modeling Transport of Mercury in Surface Water and Groundwater - Case Study in Upper East Fork Poplar Creek, Oak Ridge, TN - 13349
In this case study, groundwater/surface water modeling was used to determine efficacy of stabilization in place with hydrologic isolation for remediation of mercury contaminated areas in the Upper East Fork Poplar Creek (UEFPC) Watershed in Oak Ridge, TN. The modeling simulates the potential for mercury in soil to contaminate groundwater above industrial use risk standards and to contribute to surface water contamination. The modeling approach is unique in that it couples watershed hydrology with the total mercury transport and provides a tool for analysis of changes in mercury load related to daily precipitation, evaporation, and runoff from storms. The model also allows for simulation of colloidal transport of total mercury in surface water. Previous models for the watershed only simulated average yearly conditions and dissolved concentrations that are not sufficient for predicting mercury flux under variable flow conditions that control colloidal transport of mercury in the watershed. The transport of mercury from groundwater to surface water from mercury sources identified from information in the Oak Ridge Environmental Information System was simulated using a watershed scale model calibrated to match observed daily creek flow, total suspended solids and mercury fluxes. Mercury sources at the former Building 81-10 area, where mercury was previously retorted, were modeled using a telescopic refined mesh with boundary conditions extracted from the watershed model. Modeling on a watershed scale indicated that only source excavation for soils/sediment in the vicinity of UEFPC had any effect on mercury flux in surface water. The simulations showed that colloidal transport contributed 85 percent of the total mercury flux leaving the UEFPC watershed under high flow conditions. Simulation of dissolved mercury transport from liquid elemental mercury and adsorbed sources in soil at former Building 81-10 indicated that dissolved concentrations are orders of magnitude below a target industrial groundwater concentration beneath the source and would not influence concentrations in surface water at Station 17. This analysis addressed only shallow concentrations in soil and the shallow groundwater flow path in soil and unconsolidated sediments to UEFPC. Other mercury sources may occur in bedrock and transport though bedrock to UEFPC may contribute to the mercury flux at Station 17. Generally mercury in the source areas adjacent to the stream and in sediment that is eroding can contribute to the flux of mercury in surface water. Because colloidally adsorbed mercury can be transported in surface water, actions that trap colloids and or hydrologically isolate surface water runoff from source areas would reduce the flux of mercury in surface water. Mercury in soil is highly adsorbed and transport in the groundwater system is very limited under porous media conditions. (authors)
12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
Fractionated and purified hybrid poplar lignins as a polyol replacement in rigid polyurethane/polyisocyanurate foams
This study introduces fractionated lignin as an innovative component in the formulation of rigid polyurethane/polyisocyanurate (PUR/PIR) foams. Low-density PUR/PIR rigid foams were prepared by replacing 80% of the petrochemical-based polyol with a hybrid poplar (HP) lignin, recovered via alkaline pretreatment, and also with a fraction of this lignin, isolated via the Aqueous Lignin Purification with Hot Agents (ALPHA) process. The as-recovered HP and ALPHA-fractionated HP lignins were characterized to determine hydroxyl content, molecular weight (Mw) distribution, and pH. Both lignin-based foams met minimum standard requirements in terms of closed cell content, compression strength, and thermal conductivity. Notably, the foams made with ALPHA-based lignin outperformed the as-recovered lignin-based foams in all measured foam properties. Moreover, the ALPHA-based foam had comparable performance to the control foam (without lignin) except for density and even surpassed the control foam in closed cell content and compressive strength. This investigation of the molecular properties of lignin suggests that significant reductions in lignin average molecular weight and polydispersity (PDI) can positively impact the properties of lignin-based rigid foams.
09 BIOMASS FUELS↗
Stream discharge and water quality data for East Fork Poplar Creek beginning 2012
This data note describes 15-min discharge and in situ water quality data at two locations along East Fork Poplar Creek in east Tennessee, USA. Data records include temperature, gauge height, water surface elevation above mean sea level, and volumetric discharge. Water quality measurements include temperature, specific conductance, pH, dissolved oxygen (percent saturation and concentration), turbidity, and less extensive fDOM data at one site. The data records begin in 2012 at one site and 2015 at the second site; monitoring at both sites is ongoing (as of 2021). The goal of this data collection is to improve understanding of watershed functions, hydrologic dynamics, and material flux. The data will contribute to site conceptual and numerical models, exposure and risk evaluation, remediation selection and design, and performance monitoring. The data are publicly available and can be accessed via unique url or DOI.
54 ENVIRONMENTAL SCIENCES↗
Increasing temperature and flow management alter mercury dynamics in East Fork Poplar Creek
East Fork Poplar Creek (EFPC) is a mercury (Hg) contaminated creek in east Tennessee, USA. Stream restoration activities included the initiation of a flow management programme in 1996 in which water from a nearby lake was pumped to the head of the creek. We conducted regular water sampling for 2 years along the length of EFPC during active flow management and for 5 years after flow management stopped. Total Hg and total monomethylmercury (MMHg) concentration and flux decreased in the uppermost reaches of EFPC that were closest to the point of water addition. Most water quality parameters, including DOC concentration, remained unchanged after flow management termination. Nevertheless, SUVA 254 , a measure of dissolved organic matter (DOM) composition, increased and coincided with increased dissolved Hg (Hg D ) concentration and flux and decreased Hg solid-water partitioning coefficients throughout EFPC. Higher SUVA 254 and Hg D concentration have potential implications for bioavailability and MMHg production. Total and dissolved MMHg concentrations increased in lower reaches of EFPC after the end of flow management and these increases were most pronounced during spring and early summer when biota are more susceptible to exposure and uptake. A general warming trend in the creek after active flow management ended likely acted in concert with higher Hg D concentration to promote higher MMHg concentration. Total and dissolved MMHg concentrations were positively correlated with water temperature above a threshold value of 10°C. Concentration changes for Hg and MMHg could not be accounted for by changes in creek discharge that accompanied the cessation of flow management. In addition to the changing DOM composition in-stream, other watershed-scale factors likely contributed to the observed patterns, as these changes occurred over months rather than instantaneously after flow management stopped. Nevertheless, similar changes in MMHg have not been observed in a tributary to EFPC.
54 ENVIRONMENTAL SCIENCES↗