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NASA NTRS2010

NASA Spacecraft Fault Management Workshop Results

Fault Management is a critical aspect of deep-space missions. For the purposes of this paper, fault management is defined as the ability of a system to detect, isolate, and mitigate events that impact, or have the potential to impact, nominal mission operations. The fault management capabilities are commonly distributed across flight and ground subsystems, impacting hardware, software, and mission operations designs. The National Aeronautics and Space Administration (NASA) Discovery & New Frontiers (D&NF) Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for 5 missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that 4 out of the 5 missions studied had significant overruns due to underestimating the complexity and support requirements for fault management. As a result of this and other recent experiences, the NASA Science Mission Directorate (SMD) Planetary Science Division (PSD) commissioned a workshop to bring together invited participants across government, industry, academia to assess the state of the art in fault management practice and research, identify current and potential issues, and make recommendations for addressing these issues. The workshop was held in New Orleans in April of 2008. The workshop concluded that fault management is not being limited by technology, but rather by a lack of emphasis and discipline in both the engineering and programmatic dimensions. Some of the areas cited in the findings include different, conflicting, and changing institutional goals and risk postures; unclear ownership of end-to-end fault management engineering; inadequate understanding of the impact of mission-level requirements on fault management complexity; and practices, processes, and tools that have not kept pace with the increasing complexity of mission requirements and spacecraft systems. This paper summarizes the findings and recommendations from that workshop, as well as opportunities identified for future investment in tools, processes, and products to facilitate the development of space flight fault management capabilities.

Newhouse, Marilyn↗
NASA NTRS2010

Results from the NASA Spacecraft Fault Management Workshop: Cost Drivers for Deep Space Missions

Fault Management, the detection of and response to in-flight anomalies, is a critical aspect of deep-space missions. Fault management capabilities are commonly distributed across flight and ground subsystems, impacting hardware, software, and mission operations designs. The National Aeronautics and Space Administration (NASA) Discovery & New Frontiers (D&NF) Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for five missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that four out of the five missions studied had significant overruns due to underestimating the complexity and support requirements for fault management. As a result of this and other recent experiences, the NASA Science Mission Directorate (SMD) Planetary Science Division (PSD) commissioned a workshop to bring together invited participants across government, industry, and academia to assess the state of the art in fault management practice and research, identify current and potential issues, and make recommendations for addressing these issues. The workshop was held in New Orleans in April of 2008. The workshop concluded that fault management is not being limited by technology, but rather by a lack of emphasis and discipline in both the engineering and programmatic dimensions. Some of the areas cited in the findings include different, conflicting, and changing institutional goals and risk postures; unclear ownership of end-to-end fault management engineering; inadequate understanding of the impact of mission-level requirements on fault management complexity; and practices, processes, and tools that have not kept pace with the increasing complexity of mission requirements and spacecraft systems. This paper summarizes the findings and recommendations from that workshop, particularly as fault management development issues affect operations and the development of operations capabilities.

Newhouse, Marilyn E.↗
NASA NTRS2010

Heritage and Advanced Technology Systems Engineering Lessons Learned from NASA Deep Space Missions

In the design and development of complex spacecraft missions, project teams frequently assume the use of advanced technology systems or heritage systems to enable a mission or reduce the overall mission risk and cost. As projects proceed through the development life cycle, increasingly detailed knowledge of the advanced and heritage systems within the spacecraft and mission environment identifies unanticipated technical issues. Resolving these issues often results in cost overruns and schedule impacts. The National Aeronautics and Space Administration (NASA) Discovery & New Frontiers (D&NF) Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for 5 missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that optimistic hardware/software inheritance and technology readiness assumptions caused cost and schedule growth for four of the five missions studied. The cost and schedule growth was not found to result from technical hurdles requiring significant technology development. The projects institutional inheritance and technology readiness processes appear to adequately assess technology viability and prevent technical issues from impacting the final mission success. However, the processes do not appear to identify critical issues early enough in the design cycle to ensure project schedules and estimated costs address the inherent risks. In general, the overruns were traceable to: an inadequate understanding of the heritage system s behavior within the proposed spacecraft design and mission environment; an insufficient level of development experience with the heritage system; or an inadequate scoping of the system-wide impacts necessary to implement an advanced technology for space flight applications. The paper summarizes the study's lessons learned in more detail and offers suggestions for improving the project's ability to identify and manage the technology and heritage risks inherent in the design solution.

Barley, Bryan↗
NASA NTRS2010

Heritage Systems Engineering Lessons from NASA Deep Space Missions

In the design and development of complex spacecraft missions, project teams frequently assume the use of advanced technology systems or heritage systems to enable a mission or reduce the overall mission risk and cost. As projects proceed through the development life cycle, increasingly detailed knowledge of the advanced and heritage systems within the spacecraft and mission environment identifies unanticipated technical issues. Resolving these issues often results in cost overruns and schedule impacts. The National Aeronautics and Space Administration (NASA) Discovery & New Frontiers (D&NF) Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for 5 missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that optimistic hardware/software inheritance and technology readiness assumptions caused cost and schedule growth for all five missions studied. The cost and schedule growth was not found to be the result of technical hurdles requiring significant technology development. The projects institutional inheritance and technology readiness processes appear to adequately assess technology viability and prevent technical issues from impacting the final mission success. However, the processes do not appear to identify critical issues early enough in the design cycle to ensure project schedules and estimated costs address the inherent risks. In general, the overruns were traceable to: an inadequate understanding of the heritage system s behavior within the proposed spacecraft design and mission environment; an insufficient level of development experience with the heritage system; or an inadequate scoping of the systemwide impacts necessary to implement an advanced technology for space flight applications. The paper summarizes the study s lessons learned in more detail and offers suggestions for improving the project s ability to identify and manage the technology and heritage risks inherent in the design solution.

Barley, Bryan↗
NASA NTRS2011

COMPASS Final Report: Radioisotope Electric Propulsion (REP) Centaur Orbiter New Frontiers Mission

Radioisotope Electric Propulsion (REP) has been shown in past studies to enable missions to outer planetary bodies including the orbiting of Centaur asteroids. Key to the feasibility for REP missions are long life, low power electric propulsion (EP) devices, low mass Radioisotope Power System (RPS) and light spacecraft (S/C) components. In order to determine the key parameters for EP devices to perform these REP missions a design study was completed to design an REP S/C to orbit a Centaur in a New Frontiers (NF) cost cap. The design shows that an orbiter using several long lived (approx.200 kg xenon (Xe) throughput), low power (approx.700 W) Hall thrusters teamed with six (150 W each) Advanced Stirling Radioisotope Generators (ASRG) can deliver 60 kg of science instruments to a Centaur in 10 yr within the NF cost cap. Optimal specific impulses (Isp) for the Hall thrusters were found to be around 2000 s with thruster efficiencies over 40 percent. Not only can the REP S/C enable orbiting a Centaur (when compared to an all chemical mission only capable of flybys) but the additional power from the REP system can be used to enhance science and simplify communications. The mission design detailed in this report is a Radioisotope Power System (RPS) powered EP science orbiter to the Centaur Thereus with arrival 10 yr after launch, ending in a 1 yr science mapping mission. Along the trajectory, approximately 1.5 yr into the mission, the REP S/C does a flyby of the Trojan asteroid Tlepolemus. The total (Delta)V of the trajectory is 8.9 km/s. The REP S/C is delivered to orbit on an Atlas 551 class launch vehicle with a Star 48 B solid rocket stage

Oleson, Steven R.↗
NASA NTRS2011

Effects and Responses to Spaceflight in the Mouse Retina

Several stress environmental factors are combined in a unique fashion during spaceflight, affecting living beings widely across their physiological systems. Recently, attention has been placed on vision changes in astronauts returning from long duration missions. Alterations include hyperoptic shift, globe flattening, choroidal folds and optic disc edema, which are probably associated with increased intracranial pressure. These observations justify a better characterization of the ocular health risks associated with spaceflight. This study investigates the impact of spaceflight on the biology of the mouse retina. Within a successful tissue sharing effort, eyes from albino Balb/cJ mice aboard STS-133 were collected for histological analysis and gene expression profiling of the retina at 1 and 7 days after landing. Both vivarium and AEM (Animal Enclosure Module) mice were used as ground controls. Oxidative stress-induced DNA damage was higher in the flight samples compared to controls on R+1, and decreased on R+7. A trend toward higher oxidative and cellular stress response gene expression was also observed on R+1 compared to AEM controls, and these levels decreased on R+7. Several genes coding for key antioxidant enzymes, namely, heme-oxygenase-1, peroxiredoxin, and catalase, were among those upregulated after flight. Likewise, NF B and TGFbeta1, were upregulated in one flight specimen that overall showed the most elevated oxidative stress markers on R+1. In addition, retinas from vivarium control mice evidenced higher oxidative stress markers, NF B and TGFbeta1, likely due to the more intense illumination in vivarium cages versus the AEM. These preliminary data suggest that spaceflight represents a source of environmental stress that translates into oxidative and cellular stress in the retina, which is partially reversible upon return to Earth. Further work is needed to dissect the contribution of the various spaceflight factors (microgravity, radiation) and to evaluate the impact of the stress response on retinal health.

Zanello, Susana B.↗
NASA NTRS2014

F Ring Core Stability: Corotation Resonance Plus Antiresonance

The decades-or-longer stability of the narrow F Ring core in a sea of orbital chaos appears to be due to an unusual combination of traditional corotation resonance and a novel kind of "antiresonance". At a series of specific locations in the F Ring region, apse precession between synodic encounters with Prometheus allows semimajor axis perturbations to promptly cancel before significant orbital period changes can occur. This cancellation fails for particles that encounter Prometheus when it is near its apoapse, especially during periods of antialignment of its apse with that of the F Ring. At these times, the strength of the semimajor axis perturbation is large (tens of km) and highly nonsinusoidal in encounter longitude, making it impossible to cancel promptly on a subsequent encounter and leading to chaotic orbital diffusion. Only particles that consistently encounter Prometheus away from its apoapse can use antiresonance to maintain stable orbits, implying that the true mean motion nF of the stable core must be defined by a corotational resonance of the form nF = nP(-kappa)P/m, where (nP, kappaP) are Prometheus' mean motion and epicycle frequency. To test this hypothesis we used the fact that Cassini RSS occultations only sporadically detect a "massive" F Ring core, composed of several-cm-and-larger particles. We regressed the inertial longitudes of 24 Cassini RSS (and VGR) detections and 43 nondetections to a common epoch, using a comb of candidate nP, and then folded them modulo the anticipated m-number of the corotational resonance (Prometheus m = 110 outer CER), to see if clustering appears. We find the "true F Ring core" is actually arranged in a series of short longitudinal arcs separated by nearly empty longitudes, orbiting at a well determined semimajor axis of 140222.4 km (from 2005-2012 at least). Small particles seen by imaging and stellar occultations spread quickly in azimuth and obscure this clumpy structure. Small chaotic variations in the mean motion and/or apse longitude of Prometheus quickly become manifest in the F Ring core, and we suggest that the core must adapt to these changes for the F Ring to maintain stability over timescales of decades and longer

Cassini RSS occultations↗
NASA NTRS2015

Transient Gene and miRNA Expression Profile Changes of Confluent Human Fibroblast Cells in Space

Microgravity or an altered gravity environment from the static 1 gravitational constant has been shown to influence global gene expression patterns and protein levels in cultured cells. However, most of the reported studies conducted in space or using simulated microgravity on the ground have focused on the growth or differentiation of the cells. Whether non-dividing cultured cells will sense the presence of microgravity in space has not been specifically addressed. In an experiment conducted on the International Space Station, confluent human fibroblast cells were fixed after being cultured in space for 3 and 14 days for investigations of gene and miRNA (microRNA) expression profile changes in these cells. A fibroblast is a type of cell that synthesizes the extracellular matrix and collagen, the structural framework for tissues, and plays a critical role in wound healing and other functions. Results of the experiment showed that on Day 3, both the flown and ground cells were still proliferating slowly even though they were confluent, as measured by the expression of the protein Ki-67 positive cells, and the cells in space grew slightly faster. Gene and miRNA expression data indicated activation of NF(sub kappa)B (nuclear factor kappa-light-chain-enhancer of activated B cells) and other growth related pathways involving HGF and VEGF in the flown cells. On Day 14 when the cells were mostly non-dividing, the gene and miRNA expression profiles between the flight and ground samples were indistinguishable. Comparison of gene and miRNA expressions in the Day 3 samples in respect to Day 14 revealed that most of the changes observed on Day 3 were related to cell growth for both the flown and ground cells. Analysis of cytoskeleton changes by immunohistochemistry staining of the cells with antibodies for alpha-tubulin showed no difference between the flight and ground samples. Results of our study suggest that in true non-dividing human fibroblast cells, microgravity in space has little effect on the gene and miRNA expression. Gene and miRNA expression changes were observed in cells that were confluent, but still proliferating slowly. The faster growth in the flown cells was associated with the activation of NF(sub kappa)B pathways which triggers the expression of several growth factors and the suppression of the cell cycle checkpoint.

Zhang, Ye↗
NASA NTRS2019

The Lunar Geophysical Network Mission

Overarching Principles: Must be better than Apollo (coverage, duration, instrument performance); Learn from the Apollo experience. Lunar Geophysical Network (LGN) New Frontiers (NF)-class mission, as part of the NF-5 call. “This mission consists of several identical landers distributed across the lunar surface, each carrying geophysical instrumentation. The primary science objectives are to characterize the Moon’s internal structure, seismic activity, global heat flow budget, bulk composition, & magnetic field.” Global distribution of multiple stations. Each station should contain a seismometer, heat flow probe, electromagnetic sounder, laser retroreflector (lunar nearside). Each station must be long-lived (e.g., approximately10 years)to allow other stations (from other countries?) to be integrated with the anchor nodes to form the International Lunar Network. Why LGN? Planetary Science: Moon represents an end-member in planetary evolution (large small body, small rocky planet); Primary planetary differentiation preserved; Key to understanding terrestrial planet initial differentiation. Lunar Science: Heat flow probes yield crustal heat budget estimates; Combined with EMS (ElectroMagnetic Sounding), the temperature profile of the deep interior can be modeled along with mineralogy; Seismic and LLR (Lunar Laser Ranging) data also yield structure and compositional information of the lunar interior; High fidelity data from LGN would enhance the usefulness of the GRAIL (Gravity Recovery and Interior Laboratory) and SELENE (Selenological and Engineering Explorer) gravity data. Human Exploration: LGN must be established prior to renewed human lunar activity - we do not know the exact locations or causes of the shallow moonquakes (SMQs) - the largest magnitude seismic events recorded by Apollo (1 event per year of magnitude greater than or equal to 5); Establishing surface infrastructure near SMQ epicenters must be avoided.

Neal, C. R.↗
NASA NTRS2022

Exploring the Clouds of Venus: Science Driven Aerobot Missions to Our Sister Planet

Venus is essential to our understanding of the evolution and habitability of Earth-size planets throughout the galaxy. The selection of the VERITAS, EnVision, and DAVINCI missions by NASA and ESA in June 2021 is an important step in advancing the science. However, addressing many of the most challenging questions will require in situ platforms that can operate in the Venus environment for extended periods in order to capture the full complexity of our sister planet. Aerobots are aerial vehicles that exploit buoyancy to achieve long-duration operation in the Venus cloud layer where environmental conditions are comparatively benign. Buoyancy control, explained in more detail in a companion paper at this conference, allows aerobots to change altitude with little energy expenditure enabling new scientific measurement opportunities. These include atmospheric chemistry, dynamics, geophysical measurements of the crust and interior and geological investigations enabled by high resolution surface imaging. One aspect to our approach to defining missions that fit within the resource constraints of competitive missions is keeping the scale small. Today’s science-driven appetite for sophisticated measurements and large volumes of data is driving size upwards but advances in technology can enable aerobots that can be delivered to Venus at manageable costs. The other aspect is supporting the aerobot at Venus with orbiters providing data relay, localization and synergistic science. The recently selected orbiters, equipped with low-cost proximity relay systems routinely used at Mars may obviate the need for dedicated orbiters thereby enabling Discovery mission candidates. Four aerobot mission concepts have been defined which fit comfortably within the current New Frontiers (NF) cost cap ($900M in $FY22). One of these concepts would also be a candidate for a Discovery mission if that cost cap ($500M in $FY19) were raised. Raising the NF cost cap would enable more capable aerobot missions combining both altitude control with synergistic orbital observations. Investigations of surface geology at high resolution with subcloud NIR nightside imaging and dropsondes on the dayside of Venus could also benefit from collaborations with foreign contributions.

"O'Rourke, Joseph"↗
DOE OSTI2020

An acidic polysaccharide (AGC3) isolated from North American ginseng ( Panax quinquefolius ) suspension culture as a potential immunomodulatory nutraceutical

Polysaccharides isolated from Panax quinquefolius roots are widely used as nutraceuticals due to their immunomodulatory properties. Despite their popularity, several challenges exist in isolating ginseng root polysaccharides such as batch-to-batch structural inconsistencies and bacterial endotoxin contamination. A plant tissue culture-based platform offers a potential solution to isolate natural polysaccharide fractions with consistent chemical characteristics and reduced endotoxin content. In this study, an acidic polysaccharide fraction (AGC3) with immunomodulatory properties was isolated from Panax quinquefolius suspension cultures. The heterogeneous fraction (molecular weight: 4.81 and 32.14 kDa), purified by anion exchange chromatography, was predominantly composed of galactose (>60%) along with the presence of rhamnose, arabinose, glucose, glucuronic acid and galacturonic acid. The major glycosidic linkages were found to be t-Galp (47.7%), 4-Galp (15.6%), 2,4-Rhap (8.1%), 6-Galp (8.1%) and 4-GalAp (6.8%). Structural analyses indicated the presence of a pectic rhamnogalacturonan I polysaccharide in AGC3. AGC3 significantly (p < 0.05) stimulated RAW 264.7 murine macrophage cells and primary murine splenocytes by enhancing the production of several immunomodulatory mediators such as IL-6, TNF-α, GM-CSF and MCP-1. The results also indicated the putative roles of NF-κB (p65/RelA) and MAPK (p38) signaling pathways in the immunostimulatory response. Additionally, AGC3 induced murine splenocyte proliferation, another major indicator of immunostimulation. Overall, AGC3 has the potential to be used as an immunostimulatory nutraceutical.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Improving Miscibility of Polymer Donor and Polymer Acceptor by Reducing Chain Entanglement for Realizing 18.64 % Efficiency All Polymer Solar Cells

All-polymer solar cells have experienced rapid development in recent years by the emergence of polymerized small molecular acceptors (PSMAs). However, the strong chain entanglements of polymer donors (P D s) and polymer acceptors (P A s) decrease the miscibility of the resulting polymer mixtures, making it challenging to optimize the blend morphology. Herein, we designed three P A s, namely PBTPICm-BDD, PBTPICγ-BDD and PBTPICF-BDD, by smartly using a BDD unit as the polymerized unit to copolymerize with different Y-typed non-fullerene small molecular acceptors (NF-SMAs), thus achieving a certain degree of distortion and giving the polymer system enough internal space to reduce the entanglements of the polymer chains. Such effects increase the chances of the P D being interspersed into the acceptor material, which improve the solubility between the P D and P A . The PBTPICγ-BDD and PBTPICF-BDD displayed better miscibility with PBQx-TCl, leading to a well optimized morphology. As a result, high power conversion efficiencies (PCEs) of 17.50 % and 17.17 % were achieved for PBQx-TCl : PBTPICγ-BDD and PBQx-TCl : PBTPICF-BDD devices, respectively. In conclusion, with the addition of PYFT-o as the third component into PBQx-TCl : PBTPICγ-BDD blend to further extend the absorption spectral coverage and finely tune microstructures of the blend morphology, a remarkable PCE of 18.64 % was realized finally.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Helicity at small x: oscillations generated by bringing back the quarks

We construct a numerical solution of the recently-derived large-N c &N f small-x helicity evolution equations with the aim to establish the small-x asymptotics of the quark helicity distribution beyond the large-N c limit explored previously in the same framework. (Here N c and N f are the numbers of quark colors and flavors.) While the large-N c helicity evolution involves gluons only, the large-N c &N f evolution includes contributions from quarks as well. We find that adding quarks to the evolution makes quark helicity distribution oscillate as a function of x. Our numerical results in the large-N c &N f limit lead to the x-dependence of the flavor-singlet quark helicity distribution which is well-approximated by $$ {\left.\Delta \Sigma \left(x,{Q}^2\right)\right|}_{\mathrm{large}\hbox{-} {N}_c\&{N}_f}\sim {\left(\frac{1}{x}\right)}^{\alpha_h^q}\cos \left[{\omega}_q\ln \left(\frac{1}{x}\right)+{\varphi}_q\right]. $$. The power $α^{q}_{h}$ exhibits a weak N f -dependence, and, for all Nf values considered, remains very close to $α^{q}_{h}$(N f =0)=$\left(4/\sqrt{3}\right)\sqrt{\alpha_s{N}_c/\left(2\pi \right)}$ obtained earlier in the large-N c limit. The novel oscillation frequency ω q and phase shift φ q depend more strongly on the number of flavors N f (with ω q = 0 in the pure-glue large-N c limit). The typical period of oscillations for ΔΣ is rather long, spanning many units of rapidity. We speculate whether the oscillations we find are related to the sign variation with x seen in the strange quark helicity distribution extracted from the data.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2023

Chemical-free production of multiple high-value bioproducts from metabolically engineered transgenic sugarcane ‘oilcane’ bagasse and their recovery using nanofiltration

Bioenergy crops have potential for being a sustainable and renewable feedstock for biofuels and various value- added bioproducts. The study utilizes recently developed transgenic sugarcane (‘oilcane’) bagasse for chemical- free coproduction of high-value bioproducts, i.e., furfurals, HMF, acetic acid, cellulosic sugars, and vegetative lipids. Hydrothermal pretreatment was optimized at 210°C for 5 min to coproduce 6.91%, 2.67%, 5.07%, 2.42% and 37.82% (w/w) furfurals, HMF, acetic acid, vegetative lipids, and cellulosic sugars, respectively from lignocellulosic biomass. Additionally, nanofiltration system in-series was successfully established to recover sugars, furfurals, HMF, and acetic acid from the pretreatment liquor. 1st nanofiltration with Duracid NF mem- brane rejected ~99% sugars. Concentrated sugars with significantly reduced inhibitory products were obtained in retentate for fermentation. 2nd nanofiltration with NF90 membrane used permeate of 1st nanofiltration as feed and rejected ~ 86% furfurals. Furthermore, the work demonstrates the feasibility of coproducing and recovering multiple biochemicals from lignocellulosic biomass.

09 BIOMASS FUELS↗
DOE OSTI2022

Improving the performance of PM6 donor polymer by random ternary copolymerization of BDD and DTBT segments

Developing high-performance wide bandgap polymer donors to match the rapidly growing non-fullerene small molecule acceptors (NF-SMAs) is of great importance to further improve the photovoltaic performances of organic solar cells (OSCs). PM6 and D18 are two leading wide bandgap donor polymers, but their performances are highly sensitive to the molecular weights, which have distinct impact on the morphology and thereby the charge generation and recombination processes. Here, in this work, we reported the random ternary copolymerization of PM6 and D18 segments to develop random terpolymers with finely tuned photoelectronic properties and optimized blend morphology for high-performance OSCs. The resulting random terpolymers (PMD-5, PMD-10, PMD-15 and PMD-20) exhibited finely tuned absorptions, energy levels and blend morphology. The optimal morphology of PMD-15:L8-BO contributed to the fast exciton diffusion and dissociation, efficient charge extraction with suppressed recombination. Thus, the power conversion efficiency was improved from 17.22% of the control PM6 devices and 17.47% of the control D18 devices to 18.12% of PMD-15 based devices, which is the top performance of random terpolymers. Moreover, the PMD-15 exhibited much higher batch-to-batch reproducibility at a broad molecular weight range than that of PM6 and D18, showing promising for practical applications.

36 MATERIALS SCIENCE↗
DOE OSTI2024

Integrative Multi-PTM Proteomics Reveals Dynamic Global, Redox, Phosphorylation, and Acetylation Regulation in Cytokine-treated Pancreatic Beta Cells

Studying regulation of protein function at a systems level necessitates an understanding of the interplay among diverse post-translational modifications (PTMs). A variety of proteomics sample processing workflows are currently used to study specific PTMs but rarely characterize multiple types of PTMs from the same sample inputs. Method incompatibilities and laborious sample preparation steps complicate large-scale physiological investigations and can lead to variations in results. The single-pot, solid-phase-enhanced sample preparation (SP3) method for sample cleanup is compatible with different lysis buffers and amenable to automation, making it attractive for high-throughput multi-PTM profiling. Herein, we describe an integrative SP3 workflow for multiplexed quantification of protein abundance, cysteine thiol oxidation, phosphorylation, and acetylation. The broad applicability of this approach is demonstrated using cell and tissue samples, and its utility for studying interacting regulatory networks is highlighted in a time-course experiment of cytokine-treated ß-cells. We observed a swift response in global regulation of protein abundances consistent with rapid activation of JAK-STAT and NF-?B signaling pathways. Regulators of these pathways as well as proteins involved in their target processes displayed multi-PTM dynamics indicative of a complex cellular response stages: acute, adaptation, and chronic (prolonged stress). PARP14, a negative regulator of JAK-STAT, had multiple co-localized PTMs that may be involved in intraprotein regulatory crosstalk. Our workflow provides a high-throughput platform that can profile multi-PTMomes from the same sample set, which is valuable in unraveling the functional roles of PTMs and their co-regulation.

proteomics, PTM, automation, SP3, cysteine thiol o↗
DOE OSTI2022

Ultrathin-film composite (uTFC) membranes based on amorphous perfluoropolymers for liquid separations

Thin-film composite membranes based on ~100nm cross-linked polyamides (PAs) are state-of-the-art membranes for liquid separations such as desalination. However, the PA layer shows poor antifouling properties and instability in chlorine solutions. Herein, we show for the first time that glassy amorphous perfluoropolymers (PFPs, such as Teflon AF and Hyflon AD) can be fabricated into ultrathin film composite (uTFC) membranes with a selective layer of <20nm for desalination. Increasing the polymer concentration in the coating solutions from 0.05 mass% to 0.3 mass% increases the selective layer thickness from 9 to 25nm. When Teflon AF2400 was fabricated into 16-nm selective layers on various porous supports, the water permeance decreases from 0.37 LMH/bar for PES10k to 0.07 LMH/bar for PES1k, and the Na 2 SO 4 rejection increases from 86.6% to 95.2%. While the water permeance is lower than the commercial PA-based NF membranes, the rejection in these PFP-based membranes is comparable. Furthr, the effect of the porous support on the water permeance can be ascribed to the geometric restriction and satisfactorily described using an empirical model. Interestingly, the membranes also exhibit good Na + /Li + separation performance and stable performance for organic solvent nanofiltration, showcasing the versatility of the PFP-based uTFC membranes for various liquid separations.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗