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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 181 records · Page 10
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

Hsa{sub c}irc{sub 0}007534/miR-761/ZIC5 regulatory loop modulates the proliferation and migration of glioma cells

Highlights: • Hsa{sub c}irc{sub 0}007534 knockdown inhibited glioma cell proliferation and migration. • Hsa{sub c}irc{sub 0}007534 regulated cell proliferation and migration by sponging miR-761. • ZIC5 was a target of miR-761. • Hsa{sub c}irc{sub 0}007534 promoted glioma cell proliferation and migration via upregulating ZIC5 expression by sponging miR-761. Increasing evidences demonstrate the essential roles of circular RNAs (circRNAs) in human cancers. However, the study about the functions of circRNAs in glioma remains very limited. In the present study, we found that circRNA hsa{sub c}irc{sub 0}007534 was highly expressed in glioma tissues compared to normal brain tissues. Furthermore, we found that knockdown of hsa{sub c}irc{sub 0}007534 significantly inhibited the proliferation and migration of glioma cells. In mechanism, we showed that hsa{sub c}irc{sub 0}007534 could sponge miR-761 to repress its availability in glioma cells. We found that inhibition of miR-761 could rescue the suppressed proliferation and migration of glioma cells by hsa{sub c}irc{sub 0}007534 knockdown. Moreover, we explored the downstream mechanism and found that ZIC5 was a target of miR-761. We showed that hsa{sub c}irc{sub 0}007534 promoted the expression of ZIC5 by inhibiting miR-761 in glioma cells. And restoration of ZIC5 expression significantly reversed the effects of hsa{sub c}irc{sub 0}007534 knockdown on glioma cell proliferation and migration. In summary, our results demonstrated that hsa{sub c}irc{sub 0}007534 serves as an oncogene in glioma via promoting ZIC5 expression by repressing miR-761 availability. Our results suggested that hsa{sub c}irc{sub 0}007534/miR-761/ZIC5 regulatory loop might be a promising therapeutic target for glioma treatment.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

ST6GAL1 negatively regulates monocyte transendothelial migration and atherosclerosis development

Highlights: • ST6GAL1 expression is inversely related to atherosclerosis development. • Knockdown of ST6GAL1 in vascular endothelial cells directly promotes monocyte transendothelial migration. • ST6GAL1 overexpression inhibits TNFα-induced monocyte transendothelial migration. • β-catenin sialylation level is downregulated in inflammatory endothelial cells. Monocyte transendothelial migration is a critical step in the initial stage of atherosclerosis, in which the involvement of α-2,6 sialyltransferase 1 (ST6GAL1) has been confirmed by increasing evidence. But the direct relationship between ST6GAL1 and atherosclerosis remains incompletely uncertain. In this study, we demonstrated that the expression level of ST6GAL1 in vascular endothelium was significantly decreased in atherosclerosis development process, while obviously recovered after atherosclerosis regression. Further analysis showed that knockdown of ST6GAL1 by RNA interference in vascular endothelial cells EA. hy926 obviously promoted TNFα-triggered monocyte-transendothelial migration, whereas overexpression of ST6GAL1 strongly inhibited monocyte-transendothelial migration. Moreover, we firstly found β-catenin is a sialylated protein and its sialylation level is decreased in TNFα-treated EA. hy926 cells, suggesting that the function of ST6GAL1 in preventing both atherosclerosis development and monocyte transendothelial migration might result from the sialylation of β-catenin of endothelium. Our results suggested ST6GAL1 might be a potential target for atherosclerosis prevention and treatment.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

TRPV4 promotes the migration and invasion of glioma cells via AKT/Rac1 signaling

Highlights: • TRPV4 is upregulated in glioma, and high-level of TRPV4 indicates a worse prognosis. • TRPV4 activation enhances the cell migration and invasion of glioma. • TRPV4-induced cell motility via AKT/Rac1 signaling pathway. Experimental evidence indicates a critical role of TRPV4 (Transient Receptor Potential Vanilloid 4) in controlling the cell migratory activity of multiple tumors. However, the oncogenic role of TRPV4 in glioma still remains elusive. In this study, we tried to investigate the oncogenic role of TRPV4 in glioma. We found that the expression levels of TRPV4 were upregulated in glioma and the high levels of TRPV4 indicated a worse prognosis in patients with glioma. TRPV4 was critical for glioma migration and invasion: activating TRPV4 by agonist GSK1016790 A enhanced glioma migration and invasion, while, the specific TRPV4 antagonist HC-067047 suppressed glioma migration and invasion. Mechanically, activated TRPV4 promoted the activation of Rac1 (Ras-related C3 botulinum toxin substrate 1) by targeting the AKT for phosphorylation, then enhanced glioma migration and invasion. All these results suggested that TRPV4 accelerates glioma migration and invasion through the AKT/Rac1 signaling, and TRPV4 might be considered as a potential target for glioma therapy.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Advancing spatiotemporal forecasts of CO 2 plume migration using deep learning networks with transfer learning and interpretation analysis

Accurate and timely forecasts of CO 2 plume distribution throughout the injection and post-injection phases are crucial for detecting plume migration, assessing leakage risks, and supporting operational decisions in geologic carbon storage (GCS). Current convolutional neural network-based approaches primarily focus on spatial information and overlook temporal dependencies in plume distributions, thus limiting their ability to capture dynamic movement effects and provide accurate predictions of plume migration. In this work, we propose two deep learning models, Auto-Encoder (AE)-LSTM and Encoder-Decoder (ED)-ConvLSTM, each uniquely designed to capture both spatial and temporal features. We apply the proposed methods to forecast the dynamic distribution of CO 2 plumes based on 108 reservoir simulations over a 30-year injection and a 30-year post-injection period. The results indicate that the ED-ConvLSTM model outperforms the AE-LSTM model in accurately predicting the spatiotemporal dynamics of CO 2 plume migration, achieving R 2 values above 0.99. To provide a deeper understanding of these model predictions, we employ a gradient-based explanation method on the trained models. This approach provides insights into the influence of input variables on plume migration forecasts and uncovers the underlying prediction mechanisms of the proposed models. Furthermore, we introduce a transfer learning technique, enabling fast and accurate plume migration forecasting in the post-injection phase by leveraging the trained model during the injection phase. This reduces the necessity for extensive data collection or re-training. In conclusion, the methods proposed in our work enhances the performance and interpretability of CO 2 plume migration forecasts, thereby facilitating informed decision-making throughout the entire lifecycle of GCS applications.

58 GEOSCIENCES↗
DOE OSTI2013

Magnolol inhibits migration of vascular smooth muscle cells via cytoskeletal remodeling pathway to attenuate neointima formation

Background: Increased proliferation and migration of vascular smooth muscle cells (VSMCs) contribute importantly to the formation of both atherosclerotic and restenotic lesions. The objective of this study was to investigate the effect of magnolol on VSMC migration. Methods: The proteolytic activity of matrix metalloproteinases (MMPs) in tumor necrosis factor alpha (TNF-α) stimulated VSMCs was performed by gelatin zymography. VSMC migration was assessed by wound healing and Boyden chamber methods. Collagen induced VSMC adhesion was determined by spectrofluorimeter and stress fibers formation was evaluated by fluorescence microscope. The expression of signaling molecules involved in stress fibers formation was determined by western blot. The phosphorylation of myosin light chain (MLC20) was determined by urea-glycerol polyacrylamide gel electrophoresis. Immunohistochemistry was performed to determine the expression of β1-integrin and collagen type I in the injured carotid arteries of rats on day 35 after vascular injury. Results: VSMC migration was strongly inhibited by magnolol without affecting MMPs expression. Also, magnolol inhibited β1-integrin expression, FAK phosphorylation and RhoA and Cdc42 activation to inhibit the collagen induced stress fibers formation. Moreover, magnolol inhibited the phosphorylation of MLC20. Our in vivo results showed that magnolol inhibited β1-integrin expression, collagen type I deposition and FAK phosphorylation in injured carotid arteries without affecting MMP-2 activity. Conclusions: Magnolol inhibited VSMC migration via inhibition of cytoskeletal remodeling pathway to attenuate neointima formation. General significance: This study provides a rationale for further evaluation of magnolol for the management of atherosclerosis and restenosis. - Highlights: • Magnolol strongly inhibited migration of VSMCs. • Magnolol inhibited stress fibers formation. • MLC20 phosphorylation was also inhibited by magnolol. • Anti-migratory effect of magnolol was cytoskeletal dependent. • Magnolol inhibited β1-integrin and collagen expression in vivo.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2015

MiR-145, a microRNA targeting ADAM17, inhibits the invasion and migration of nasopharyngeal carcinoma cells

MiR-145 is downregulated and functions as a tumor suppressor in many malignancies. In this study, the biological function, molecular mechanism, and direct target genes of miR-145 in nasopharyngeal carcinoma (NPC) cells were investigated. Cell survival was detected by cell viability assay, and cell cycle was determined through flow cytometry. Invasion and migration of NPC cells were examined using cell invasion and wound healing assays, respectively. A disintegrin and metalloproteinase 17 (ADAM17) was verified as the target of miR-145 through luciferase reporter assay, qRT-PCR, and Western blot analysis. In NPC cell lines, miR-145 expression was significantly downregulated and ADAM17 protein expression was upregulated. ADAM17 was downregulated at the post-transcriptional level by miR-145 via the binding site of ADAM17-3′UTR. Transfection with miR-145 mimic suppressed cell growth and induced cell cycle arrest in the G0/G1 phase by upregulating key G0/G1 phase regulators, namely, p53 and p21. MiR-145 also inhibited cellular migration and invasion through targeting ADAM17 involving the regulation of EGFR and E-cadherin. Knockdown of ADAM17 elicited similar effects to that of miR-145 on NPC cells. This study reveals that miR-145 suppressed the invasion and migration of NPC cells by targeting ADAM17. Thus, miR-145 could be a therapeutic target for NPC. - Highlights: • MiR-145 inhibits proliferation, invasion, and migration in NPC cells. • Knockdown of ADAM17 inhibits invasion and migration in NPC cells. • MiR-145 inhibits invasion and migration in NPC cells by targeting ADAM17.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

Collective epithelial cell sheet adhesion and migration on polyelectrolyte multilayers with uniform and gradients of compliance

Polyelectrolyte multilayers (PEMUs) are tunable thin films that could serve as coatings for biomedical implants. PEMUs built layer by layer with the polyanion poly(acrylic acid) (PAA) modified with a photosensitive 4-(2-hydroxyethoxy) benzophenone (PAABp) group and the polycation poly(allylamine hydrochloride) (PAH) are mechanically tunable by UV irradiation, which forms covalent bonds between the layers and increases PEMU stiffness. PAH-terminated PEMUs (PAH-PEMUs) that were uncrosslinked, UV-crosslinked to a uniform stiffness, or UV-crosslinked with an edge mask or through a neutral density optical gradient filter to form continuous compliance gradients were used to investigate how differences in PEMU stiffness affect the adhesion and migration of epithelial cell sheets from scales of the fish Poecilia sphenops (Black Molly) and Carassius auratus (Comet Goldfish). During the progressive collective cell migration, the edge cells (also known as ‘leader’ cells) in the sheets on softer uncrosslinked PEMUs and less crosslinked regions of the gradient formed more actin filaments and vinculin-containing adherens junctions and focal adhesions than formed in the sheet cells on stiffer PEMUs or glass. During sheet migration, the ratio of edge cell to internal cell (also known as ‘follower’ cells) motilities were greater on the softer PEMUs than on the stiffer PEMUs or glass, causing tension to develop across the sheet and periods of retraction, during which the edge cells lost adhesion to the substrate and regions of the sheet retracted toward the more adherent internal cell region. These retraction events were inhibited by the myosin II inhibitor Blebbistatin, which reduced the motility velocity ratios to those for sheets on the stiffer PEMUs. Blebbistatin also caused disassembly of actin filaments, reorganization of focal adhesions, increased cell spreading at the leading edge, as well as loss of edge cell-cell connections in epithelial cell sheets on all surfaces. Interestingly, cells throughout the interior region of the sheets on uncrosslinked PEMUs retained their actin and vinculin organization at adherens junctions after treatment with Blebbistatin. Like Blebbistatin, a Rho-kinase (ROCK) inhibitor, Y27632, promoted loss of cell-cell connections between edge cells, whereas a Rac1 inhibitor, NSC23766, primarily altered the lamellipodial protrusion in edge cells. Compliance gradient PAH-PEMUs promoted durotaxis of the cell sheets but not of individual keratocytes, demonstrating durotaxis, like plithotaxis, is an emergent property of cell sheet organization. - Highlights: • Fish scale cell sheets migrate on PAH-PAABp polyelectrolyte multilayers. • Sheets migrating on softer PEMUs periodically retract. • Sheets durotax on modulus gradients. • Myosin II inhibitors inhibit sheet integrity and migration.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

Pediatric glioblastoma cells inhibit neurogenesis and promote astrogenesis, phenotypic transformation and migration of human neural progenitor cells within cocultures

Highlights: • Interactions between human NPCs and human pediatric GBM-derived cells investigated using a coculture setup. • NPC morphology, phenotype, differentiation, neurite outgrowth, and migration patterns significantly influenced by GBMs. • Cumulative migration distance fit to Furth's formula derived formally from Ornstein-Uhlenbeck process. • Significant applications in NPC transplantation efforts to replace lesions of excised tumor sites. • Cocultures helped elucidate the underlying pathways involved in GBM-NPC interactions in a tumor microenvironment. Neural progenitor cell (NPC) fate is influenced by a variety of biological cues elicited from the surrounding microenvironment and recent studies suggest their possible role in pediatric glioblastoma multiforme (GBM) development. Since a few GBM cells also display NPC characteristics, it is not clear whether NPCs transform to tumor cell phenotype leading to the onset of GBM formation, or NPCs migrate to developing tumor sites in response to paracrine signaling from GBM cells. Elucidating the paracrine interactions between GBM cells and NPCs in vivo is challenging due to the inherent complexity of the CNS. Here, we investigated the interactions between human NPCs (ReNcell) and human pediatric GBM-derived cells (SJ-GBM2) using a Transwell{sup ®} coculture setup to assess the effects of GBM cells on ReNcells (cytokine and chemokine release, viability, phenotype, differentiation, migration). Standalone ReNcell or GBM cultures served as controls. Qualitative and quantitative results from ELISA{sup ®}, Live/Dead{sup ®} and BrdU assays, immunofluorescence labeling, western blot analysis, and scratch test suggests that although ReNcell viability remained unaffected in the presence of pediatric GBM cells, their morphology, phenotype, differentiation patterns, neurite outgrowth, migration patterns (average speed, distance, number of cells) and GSK-3β expression were significantly influenced. The cumulative distance migrated by the cells in each condition was fit to Furth's formula, derived formally from Ornstein-Uhlenbeck process. ReNcell differentiation into neural lineage was compromised and astrogenesis promoted within cocultures. Such coculture platform could be extended to identify the specific molecules contributing to the observed phenomena, to investigate whether NPCs could be transplanted to replace lesions of excised tumor sites, and to elucidate the underlying molecular pathways involved in GBM-NPC interactions within the tumor microenvironment.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

Mechano-growth factor protects against mechanical overload induced damage and promotes migration of growth plate chondrocytes through RhoA/YAP pathway

Highlights: • Cyclic mechanical stress induces MGF expression and MGF protects against mechanical overload induced apoptosis and inflammation. • MGF regulates YAP activation to induce chondrocytes migration via RhoA. • MGF promotes focal adhesion formation via RhoA-YAP pathway. Epiphyseal growth plate is highly dynamic tissue which is controlled by a variety of endocrine, paracrine hormones, and by complex local signaling loops and mechanical loading. Mechano growth factor (MGF), the splice variant of the IGF-I gene, has been discovered to play important roles in tissue growth and repair. However, the effect of MGF on the growth plate remains unclear. In the present study, we found that MGF mRNA expression of growth plate chondrocytes was upregulated in response to mechanical stimuli. Treatment of MGF had no effect on growth plate chondrocytes proliferation and differentiation. But it could inhibit growth plate chondrocytes apoptosis and inflammation under mechanical overload. Moreover, both wound healing and transwell assay indicated that MGF could significantly enhance growth plate chondrocytes migration which was accompanied with YAP activation and nucleus translocation. Knockdown of YAP with YAP siRNA suppressed migration induced by MGF, indicating the essential role of YAP in MGF promoting growth plate chondrocytes migration. Furthermore, MGF promoted YAP activation through RhoA GTPase mediated cytoskeleton reorganization, RhoA inhibition using C3 toxin abrogated MGF induced YAP activation. Importantly, we found that MGF promoted focal adhesion(FA) formation and knockdown of YAP with YAP siRNA partially suppressed the activation of FA kinase, implying that YAP is associated with FA formation. In conclusion, MGF is an autocrine growth factor which is regulated by mechanical stimuli. MGF could not only protect growth plate chondrocytes against damage by mechanical overload, but also promote migration through activation of RhoA/YAP signaling axis. Most importantly, our findings indicate that MGF promote cell migration through YAP mediated FA formation to determine the FA-cytoskeleton remodeling.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

The actomyosin network is influenced by NMHC IIA and regulated by CrpF46, which is involved in controlling cell migration

Highlights: • Crp{sup F46} knockdown in cells hindered cell migration. • Crp{sup F46} knockdown disturbed MT arrangement and centrosomal reorientation. • Crp{sup F46} knockdown induced weak stress fiber formation. • Crp{sup F46} interacted with NMHC IIA with its three coiled-coil domains. • Myosin filaments are regulated by Crp{sup F46} associated with phosphorylation of myosin. When a cell migrates, the centrosome positions between the nucleus and the leading edge of migration via the microtubule system. The protein Crp{sup F46} (centrosome-related protein F46) has a known role during mitosis and centrosome duplication. However, how Crp{sup F46} efficiently regulates centrosome-related cell migration is unclear. Here, we report that knockdown of Crp{sup F46} resulted in the disruption of microtubule arrangement, with impaired centrosomal reorientation, and slowed down cell migration. In cells that express low levels of Crp{sup F46}, stress fibers were weakened, which could be rescued by recovering Flag-Crp{sup F46}. We also found that Crp{sup F46} interacted with non-muscle myosin high chain IIA (NMHC IIA) and that its three coiled-coil domains are pivotal for its binding to NMHC IIA. Additionally, analyses of phosphorylation of NMHC IIA and RLC (regulatory light chain) demonstrated that Crp{sup F46} was associated with myosin IIA during filament formation. Indirect immunofluorescence images indicated that NM IIA filaments were inhibited when Crp{sup F46} was under-expressed. Thus, Crp{sup F46} regulates cell migration by centrosomal reorientation and altering the function of the actomyosin network by controlling specific phosphorylation of myosin.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2019

Time-resolved molecular dynamics of single and double hydrogen migration in ethanol

Being the lightest, most mobile atom that exists, hydrogen plays an important role in the chemistry of hydrocarbons, proteins and peptides and most biomolecules. Hydrogen can undergo transfer, exchange and migration processes, having considerable impact on the chemical behavior of these molecules. Although much has been learned about reaction dynamics involving one hydrogen atom, less is known about those processes where two or more hydrogen atoms participate. Here we show that single and double hydrogen migrations occurring in ethanol cations and dications take place within a few hundred fs to ps, using a 3D imaging and laser pump-probe technique. For double hydrogen migration, the hydrogens are not correlated, with the second hydrogen migration promoting the breakup of the C–O bond. The probability of double hydrogen migration is quite significant, suggesting that double hydrogen migration plays a more important role than generally assumed. The conclusions are supported by state-of-the-art molecular dynamics calculations.

08 HYDROGEN↗
DOE OSTI2020

Voltage decay and redox asymmetry mitigation by reversible cation migration in lithium-rich layered oxide electrodes

Despite the high energy density of lithium-rich layered-oxide electrodes, their real-world implementation in batteries is hindered by the substantial voltage decay on cycling. This voltage decay is widely accepted to mainly originate from progressive structural rearrangements involving irreversible transition-metal migration. As prevention of this spontaneous cation migration has proven difficult, a paradigm shift toward management of its reversibility is needed. Herein, we demonstrate that the reversibility of the cation migration of lithium-rich nickel manganese oxides can be remarkably improved by altering the oxygen stacking sequences in the layered structure and thereby dramatically reducing the voltage decay. The preeminent intra-cycle reversibility of the cation migration is experimentally visualized, and first-principles calculations reveal that an O2-type structure restricts the movements of transition metals within the Li layer, which effectively streamlines the returning migration path of the transition metals. Furthermore, we propose that the enhanced reversibility mitigates the asymmetry of the anionic redox in conventional lithium-rich electrodes, promoting the high-potential anionic reduction, thereby reducing the subsequent voltage hysteresis. Our findings demonstrate that regulating the reversibility of the cation migration is a practical strategy to reduce voltage decay and hysteresis in lithium-rich layered materials.

25 ENERGY STORAGE↗
DOE OSTI2014

THE MIGRATION OF GAP-OPENING PLANETS IS NOT LOCKED TO VISCOUS DISK EVOLUTION

Most standard descriptions of Type II migration state that massive, gap-opening planets must migrate at the viscous drift rate. This is based on the idea that the disk is separated into an inner and outer region and gas is considered unable to cross the gap. In fact, gas easily crosses the gap on horseshoe orbits, nullifying this necessary premise which would set the migration rate. In this work, it is demonstrated using highly accurate numerical calculations that the actual migration rate is dependent on disk and planet parameters, and can be significantly larger or smaller than the viscous drift rate. In the limiting case of a disk much more massive than the secondary, the migration rate saturates to a constant that is sensitive to disk parameters and is not necessarily of the order of the viscous rate. In the opposite limit of a low-mass disk, the migration rate decreases linearly with disk mass. Steady-state solutions in the low disk mass limit show no pile-up outside the secondary's orbit, and no corresponding drainage of the inner disk.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

Surface coating for prevention of metallic seed migration in tissues

Purpose: In radiotherapy, metallic implants often detach from their deposited sites and migrate to other locations. This undesirable migration could cause inadequate dose coverage for permanent brachytherapy and difficulties in image-guided radiation delivery for patients. To prevent migration of implanted seeds, the authors propose a potential strategy to use a biocompatible and tissue-adhesive material called polydopamine. Methods: In this study, nonradioactive dummy seeds that have the same geometry and composition as commercial I-125 seeds were coated in polydopamine. Using scanning electron microscopy and x-ray photoelectron spectroscopy, the surface of the polydopamine-coated and noncoated seeds was characterized. The detachment stress between the two types of seeds and the tissue was measured. The efficacy of polydopamine-coated seed was investigated through in vitro migration tests by tracing the seed location after tissue implantation and shaking for given times. The cytotoxicity of the polydopamine coating was also evaluated. Results: The results of the coating characterization have shown that polydopamine was successfully coated on the surface of the seeds. In the adhesion test, the polydopamine-coated seeds had 2.1-fold greater detachment stress than noncoated seeds. From the in vitro test, it was determined that the polydopamine-coated seed migrated shorter distances than the noncoated seed. This difference was increased with a greater length of time after implantation. Conclusions: The authors suggest that polydopamine coating is an effective technique to prevent migration of implanted seeds, especially for permanent prostate brachytherapy.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

SU-F-T-675: Down-Regulating the Expression of Cdc42 and Inhibition of Migration of A549 with Combined Treatment of Ionizing Radiation and Sevoflurane

Purpose: Cdc42 is involved in cell transformation, proliferation, invasion and metastasis of human cancer cells. Cdc42 overexpression has been reported in several types of cancers. This study investigated the combined treatment effects of ionizing radiation and sevoflurane on down-regulating Cdc42 expression and suppressing migration of human adenocarcinoma cell line A549. Methods: Samples of A549 cells with Cdc42 overexpression were created and Cdc42 expression was determined by Western blotting. Increase of migration speed by Cdc42-HA overexpression was confirmed with an initial in-vitro scratch assay. The cells grown in culture media were separated into 2 groups of 6 samples: one for the control and the other was treated with 4% sevoflurane for 5hrs prior to a single-fraction radiation of 4Gy using a 6MV beam. Cell migration speeds of the 2 groups were measured with an initial in-vitro scratch assay. The scratch was created with a pipette tip immediately after treatment and images at 4 post-treatment time points (0h, 3h, 6h, 12h) were acquired. The distance between the two separated sides at 0h was used as reference and subsequent changes of the distance over time was defined as the cell migration speed. Image processing and measurement were performed with an in-house software. The experiment was repeated three times independently to evaluate the repeatability and reliability. Statistical analysis was performed with SPSS 19.0. Results: Western blotting showed the treatment down-regulated Cdc42 overexpression. Quantitative analysis and two-tailed t-test showed that cell migration speed of the treated group was higher than the control group at all time points after treatment (p < 0.02). Conclusion: Combined treatment of 6MV photon and sevoflurane can cause the effects of down-regulating Cdc42 overexpression and decrease of migration speed of A549 cells which provides potential of clinical benefit for the cancer therapy. More investigation is needed to further quantify the benefits of the combined therapy.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

Developing high-resolution spatial data of migration corridors for avian species of concern in regions of high potential wind development

The future of the US economy, our national security, and our environmental quality all depend on decreasing our reliance on foreign oil and on fossil fuels. An essential component of decreasing this reliance is the development of alternative energy sources. Wind power is among the most important alternative energy sources currently available, and the mid-Atlantic region is a primary focus for wind power development. In addition to being important to the development of wind power, the mid-Atlantic region holds a special responsibility for the conservation of the eastern North America's golden eagles (Aquila chrysaetos). This small population breeds in northeastern Canada, winters in the southern Appalachians, and nearly all of these birds pass through the mid-Atlantic region twice each year. Movement of these birds is not random and, particularly during spring and autumn, migrating golden eagles concentrate in a narrow 30-50 mile wide corridor in central Pennsylvania. Thus, because the fate of these rare birds may depend on responsible management of the habitat they use it is critical to use research to identify ways to mitigate prospective impacts on this and similar raptor species. The goal of this project was to develop high-resolution spatial risk maps showing migration corridors of and habitat use by eastern golden eagles in regions of high potential for wind development. To accomplish this, we first expanded existing models of raptor migration for the eastern USA to identify broad-scale migration patterns. We then used data from novel high-resolution tracking devices to discover routes of passage and detailed flight behavior of individual golden eagles throughout the eastern USA. Finally, we integrated these data and models to predict population-level migration patterns and individual eagle flight behavior on migration. We then used this information to build spatially explicit, probabilistic maps showing relative risk to birds from wind development. This project has numerous benefits to people and to wildlife, primarily because it will provide a framework for safer and less controversial development of wind power. Because golden eagles are an important "umbrella" for other raptors, this project benefits a suite of species that may be impacted by wind turbines. Finally this work is a recognized priority for central Appalachian states and it is explicitly called for in, and meets the goals of, numerous state wildlife conservation plans. The final product we created, a region-wide map of relative risk to eagles of development of wind power, has allowed us to make specific recommendations regarding siting and operation of and mitigation at wind facilities. This approach also serves as a model for other projects to protect eagles in other places and to conserve suites of species beyond raptors.

17 WIND ENERGY↗
DOE OSTI2017

Final Project Report: Imaging Fault Zones Using a Novel Elastic Reverse-Time Migration Imaging Technique

Imaging fault zones and fractures is crucial for geothermal operators, providing important information for reservoir evaluation and management strategies. However, there are no existing techniques available for directly and clearly imaging fault zones, particularly for steeply dipping faults and fracture zones. In this project, we developed novel acoustic- and elastic-waveform inversion methods for high-resolution velocity model building. In addition, we developed acoustic and elastic reverse-time migration methods for high-resolution subsurface imaging of complex subsurface structures and steeply-dipping fault/fracture zones. We first evaluated and verified the improved capabilities of our newly developed seismic inversion and migration imaging methods using synthetic seismic data. Our numerical tests verified that our new methods directly image subsurface fracture/fault zones using surface seismic reflection data. We then applied our novel seismic inversion and migration imaging methods to a field 3D surface seismic dataset acquired at the Soda Lake geothermal field using Vibroseis sources. Our migration images of the Soda Lake geothermal field obtained using our seismic inversion and migration imaging algorithms revealed several possible fault/fracture zones. AltaRock Energy, Inc. is working with Cyrq Energy, Inc. to refine the geologic interpretation at the Soda Lake geothermal field. Trenton Cladouhos, Senior Vice President R&D of AltaRock, was very interested in our imaging results of 3D surface seismic data from the Soda Lake geothermal field. He planed to perform detailed interpretation of our images in collaboration with James Faulds and Holly McLachlan of University of Nevada at Reno. Using our high-resolution seismic inversion and migration imaging results can help determine the optimal locations to drill wells for geothermal energy production and reduce the risk of geothermal exploration.

47 OTHER INSTRUMENTATION↗
DOE OSTI2020

Climate change as an incentive for future human migration

Human migration is both motivated and constrained by a multitude of socioeconomic and environmental factors, including climate-related factors. Climatic factors exert an influence on local and regional population density. Here, we examine the implications of future motivation for humans to migrate by analyzing today's relationships between climatic factors and population density, with all other factors held constant. Such “all other factors held constant” analyses are unlikely to make quantitatively accurate predictions, but the order of magnitude and spatial pattern that come out of such an analysis can be useful when considering the influence of climate change on the possible scale and pattern of future incentives to migrate. Our results indicate that, within decades, climate change may provide hundreds of millions of people with additional incentive to migrate, largely from warm tropical and subtropical countries to cooler temperate countries, with India being the country with the greatest number of people with additional incentive to migrate. These climate-driven incentives would be among the broader constellation of incentives that influence migration decisions. Areas with the highest projected population growth rates tend to be areas that are likely to be most adversely affected by climate change.

54 ENVIRONMENTAL SCIENCES↗