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At least 181 records · Page 10
All sourcesNASA NTRSDOE OSTI
NASA NTRS1996

The effect of in vivo and in vitro irradiation (25 Gy) on the subsequent in vitro growth of satellite cells

The effect of in vivo and in vitro irradiation on subsequent satellite cell growth, in vitro, was investigated to ascertain the ability of a 25 Gy dose to inhibit satellite cell proliferation. Satellite cells were isolated from the left (irradiated) and right (non-irradiated) Pectoralis thoracicus of two-week-old tom turkeys 16 h (n=3) and seven weeks (n=2) after the left Pectoralis thoracicus had been irradiated (25 Gy). Satellite cells isolated from the irradiated and non-irradiated muscles exhibited similar (P>0.10) in vitro proliferation indicating that a population of satellite cells survived an in vivo dose of 25 Gy. In additional experiments, satellite cell cultures derived from tom turkey Pectoralis thoracicus were irradiated (25 Gy) in vitro. The number of satellite cells did not (P>0.05) increase in irradiated cultures for 134 h following irradiation, while satellite cells in non-irradiated cultures proliferated (P<0.05) over this time. At later time periods, satellite cell number increased (P<0.05) in irradiated cultures indicating that a population of satellite cells survived irradiation. The results of these in vitro experiments suggest that a 25 Gy dose of irradiation does not abolish satellite cell divisions in the turkey Pectoralis thoracicus.

NASA Discipline Musculoskeletal↗
NASA NTRS1990

Long-term Solar Irradiance Variability: 1984-1989 Observations

Long-term variability in the total solar irradiance has been observed in the Earth Radiation Budget Experiment (ERBE) solar monitor measurements. The monitors have been used to measure the irradiance from the Earth Radiation Budget Satellite (ERBS) and the National Oceanic and Atmospheric Administration NOAA-9 and NOAA-10 spacecraft platforms since October 25, 1984, January 23, 1985, and October 22, 1986, respectively. Before September 1986, the ERBS irradiance values were found to be decreasing -0.03 percent per year. This period was marked by decreasing solar magnetic activity. Between September 1986 and mid-1989, the irradiance values increased approximately 0.1 percent. The latter period was marked by increasing solar activity which was associated with the initiations of the sunspot cycle number 22 and of a new 22-year Hale solar magnetic cycle. Therefore, long-term solar-irradiance variability appears to be correlated directly with solar activity. The maximum smoothed sunspot number occurred during September 1989, according to the Sunspot Index Data Center. Therefore, the recent irradiance increasing trend should disappear during early 1990 and change into a decreasing trend if the observed irradiance variability is correlated more so with the 11-year sunspot cycle than the 22-year Hale cycle. The ERBE irradiance values are presented and compared with sunspot activity for the 1984 to 1989 period. The ERBE values are compared with those available from the Nimbus-7 and Solar Maximum Mission spacecraft experiments.

Lee, Robert B., III↗
NASA NTRS2000

Principal Component Analysis of Arctic Solar Irradiance Spectra

During the FIRE (First ISCPP Regional Experiment) Arctic Cloud Experiment and coincident SHEBA (Surface Heat Budget of the Arctic Ocean) campaign, detailed moderate resolution solar spectral measurements were made to study the radiative energy budget of the coupled Arctic Ocean - Atmosphere system. The NASA Ames Solar Spectral Flux Radiometers (SSFRs) were deployed on the NASA ER-2 and at the SHEBA ice camp. Using the SSFRs we acquired continuous solar spectral irradiance (380-2200 nm) throughout the atmospheric column. Principal Component Analysis (PCA) was used to characterize the several tens of thousands of retrieved SSFR spectra and to determine the number of independent pieces of information that exist in the visible to near-infrared solar irradiance spectra. It was found in both the upwelling and downwelling cases that almost 100% of the spectral information (irradiance retrieved from 1820 wavelength channels) was contained in the first six extracted principal components. The majority of the variability in the Arctic downwelling solar irradiance spectra was explained by a few fundamental components including infrared absorption, scattering, water vapor and ozone. PCA analysis of the SSFR upwelling Arctic irradiance spectra successfully separated surface ice and snow reflection from overlying cloud into distinct components.

Rabbette, Maura↗
NASA NTRS2011

How Does the Sun's Spectrum Vary?

Recent observations made by the Spectral Irradiance Monitor (SIM) on the Solar Radiation and Climate Experiment (SORCE) spacecraft suggest that the Sun's visible and infrared spectral irradiance increased from 2004 to 2008, even as the total solar irradiance measured simultaneously by SORCE's Total Irradiance Monitor (TIM) decreased. As well, solar ultraviolet (UV) irradiance decreased 3 to 10 times more than expected from prior observations and model calculations of the known effects of sunspot and facular solar features. Analysis of the SIM spectral irradiance observations during the solar minimum epoch of 2008, when solar activity was essentially invariant, exposes trends in the SIM observations relative to both total solar irradiance and solar activity that are unlikely solar in origin. We suggest that the SIM's radically different solar variability characterization is a consequence of undetected instrument sensitivity drifts, not true solar spectrum changes. It is thus doubtful that simulations of climate and atmospheric change using SIM measurements are indicative of real behavior in the Earth's climate and atmosphere.

Lean, Judith L.↗
NASA NTRS1971

Radiation and zero-gravity effects on human leukocytes and Neurospora crassa

The two parts of this experiment have resulted in proof that neither orbital space flight nor any of the stresses connected with it produced significant, unpredicted genetic damage, at least insofar as chromosomal-aberration production is a valid measure of this general type of effect. Furthermore, the Gemini 11 results lead to the conclusion that no synergistic effect exists between radiation and factors that are associated with space flight. No significant difference was found between dose-effect curves for survival or mutation induction of the inflight and ground-based samples irradiated on filters. Thus, like the blood experiment, this part of the experiment failed to result in data that were appropriate for confirmation of the apparent synergism that was observed in the Gemini 3 blood experiment. Also, the Nuerospora crassa experiment provided conclusive data that there is no difference in the genetic effects of irradiation during space flight and the genetic effects obtained in ground-based experiments. Both the Gemini 11 blood experiment and the Neurospora crassa experiment thus have failed to result in data confirmatory of the apparent synergism observed on the Gemini 3 mission.

Bender, M. A.↗
NASA NTRS2017

Radiation Chemistry of Solid Acetone in the Interstellar Medium - A New Dimension to an Old Problem

A laboratory investigation of acetone, an interstellar and cometary molecule, has produced new results concerning its decomposition in a radiation environment. Mid-infrared spectroscopy has been used to follow amorphous acetone's destruction by ionizing radiation (1 MeV protons) at 20 K. Radiation products identified are the CH4, CO, and CO2 usually made in such experiments, along with ketene, allene, and the acetonyl radical, all identified here for the first time in irradiated solid acetone. Evidence for the reduction product 2-propanol was suggestive, but a firm identification could not be made either for it or for the C2 hydrocarbons (i.e., C2H6, C2H4, C2H2). The acetyl radical was not observed as a radiation product. Isotopically labeled reagents were used to demonstrate ketene formation and to emphasize that multiple approaches are needed for robust assignments of infrared spectral features of irradiated icy solids. Results from a supporting radiation experiment with isotopically labeled acetic acid are described. Comparisons are made to a previous study of acetone's stability in extraterrestrial radiation environments, and caution is urged in measuring and interpreting CO abundances in irradiated icy solids.

cometary molecule↗
NASA NTRS2017

Radiation Chemistry of Solid Acetone in the Interstellar Medium - A New Dimension to an Old Problem

A laboratory investigation of acetone, an interstellar and cometary molecule, has produced new results concerning its decomposition in a radiation environment. Mid-infrared spectroscopy has been used to follow amorphous acetone’s destruction by ionizing radiation (1 MeV protons) at 20 K. Radiation products identified are the CH4, CO, and CO2 usually made in such experiments, along with ketene, allene, and the acetonyl radical, all identified here for the first time in irradiated solid acetone. Evidence for the reduction product 2-propanol was suggestive, but a firm identification could not be made either for it or for the C2 hydrocarbons (i.e., C2H6, C2H4, C2H2). The acetyl radical was not observed as a radiation product. Isotopically labeled reagents were used to demonstrate ketene formation and to emphasize that multiple approaches are needed for robust assignments of infrared spectral features of irradiated icy solids. Results from a supporting radiation experiment with isotopically labeled acetic acid are described. Comparisons are made to a previous study of acetone’s stability in extraterrestrial radiation environments, and caution is urged in measuring and interpreting CO abundances in irradiated icy solids.

R. L. Hudson↗
NASA NTRS1994

Inactivation, DNA double strand break induction and their rejoining in bacterial cells irradiated with heavy ions

Besides inactivation one of the major interests in our experiments is to study the primary damage in the DNA double strand breaks (DSB) after heavy ion irradiation. These damages lead not only to cell death but also under repair activities to mutations. In further experiments we have investigated the inactivation with two different strains of Deinococcus radiodurans (R1, Rec 30) and the induction of DSB as well as the rejoining of DSB in stationary cells of E. coli (strain B/r) irradiated with radiations of different quality. In the latter case irradiations were done so that the cell survival was roughly at the same level. We measured the DSB using the pulse field gelelectrophoresis which allows to separate between intact (circular) and damaged (linear) DNA. The irradiated cells were transferred to NB medium and incubated for different times to allow rejoining.

Schaefer, M.↗
NASA NTRS2011

Solar EUV Variability from FISM and SDO/EVE During Solar Minimum, Active, and Flaring Time Periods

The Living With a Star (LWS) Focus Science Team has identified three periods of different solar activity levels for which they will be determining the Earth's Ionosphere and Thermosphere response. Not only will the team be comparing individual models (e.g. FLIP, T1MEGCM, GLOW) outcome driven by the various levels of solar activity, but the models themselves will also be compared. These models all rely on the input solar EUV (0.1 -190 nm) irradiance to drive the variability. The Flare Irradiance Spectral Model (FISM) and the EUV Variability Experiment (EVE) onboard provide the Solar Dynamics Observatory (SDO) provide the most accurate quantification of these irradiances. Presented and discussed are how much the solar EUV irradiance changes during these three scenarios, both as a function of activity and wavelength.

Chamberlin, Phillip C.↗
NASA NTRS1979

Improvement and extension of data from ATS-6 Solar Cell Radiation Damage Experiment (SCRDE)

The ATS 6 solar cell radiation damage experiment data through 2 1/3 years of synchronous orbit operation are presented. Comparisons are made of the performances of the 13 different types of solar cell/cover configurations, including solar cell and cover thickness variations, base resistivity variation, new cover processes and materials, and the COMSAT violet cell. These performances are also compared to the performance of the LES 6 solar cell experiment, the ATS 6 main solar arrays, and laboratory spectrum electron irradiations. It is found that the cells of the ATS 6 experiment generally performed as expected through 6 to 9 months in orbit, but that at 2 1/3 years they were severely degraded in current. The short circuit current degradation after 2 1/3 years in orbit appears to exhibit an anomalous additional degradation of 5 to 9 percent over what was experienced in synchronous orbit operation.

Goldhammer, L. J.↗
NASA NTRS2012

A Multiscale Computational Model of the Response of Swine Epidermis After Acute Irradiation

Radiation exposure from Solar Particle Events can lead to very high skin dose for astronauts on exploration missions outside the protection of the Earth s magnetic field [1]. Assessing the detrimental effects to human skin under such adverse conditions could be predicted by conducting territorial experiments on animal models. In this study we apply a computational approach to simulate the experimental data of the radiation response of swine epidermis, which is closely similar to human epidermis [2]. Incorporating experimentally measured histological and cell kinetic parameters into a multiscale tissue modeling framework, we obtain results of population kinetics and proliferation index comparable to unirradiated and acutely irradiated swine experiments [3]. It is noted the basal cell doubling time is 10 to 16 days in the intact population, but drops to 13.6 hr in the regenerating populations surviving irradiation. This complex 30-fold variation is proposed to be attributed to the shortening of the G1 phase duration. We investigate this radiation induced effect by considering at the sub-cellular level the expression and signaling of TGF-beta, as it is recognized as a key regulatory factor of tissue formation and wound healing [4]. This integrated model will allow us to test the validity of various basic biological rules at the cellular level and sub-cellular mechanisms by qualitatively comparing simulation results with published research, and should lead to a fuller understanding of the pathophysiological effects of ionizing radiation on the skin.

FROM↗
NASA NTRS1986

Observed perturbations of the Earth's Radiation Budget - A response to the El Chichon stratospheric aerosol layer?

The Earth Radiation Budget experiment, launched aboard the Nimbus-7 polar-orbiting spacecraft in late 1978, has now taken over seven years of measurements. The dataset, which is global in coverage, consists of the individual components of the earth's radiation budget, including longwave emission, net radiation, and both total and near-infrared albedos. Starting some six months after the 1982 eruption of the El Chichon volcano, substantial long-lived positive shortwave irradiance anomalies were observed by the experiment in both the northern and southern polar regions. Analysis of the morphology of this phenomena indicates that the cause is the global stratospheric aerosol layer which formed from the cloud of volcanic effluents. There was little change in the emitted longwave in the polar regions. At the north pole the largest anomaly was in the near-infrared, but at the south pole the near UV-visible anomaly was larger. Assuming an exponential decay, the time constant for the north polar, near-infrared anomaly was 1.2 years. At mid- and low latitudes the effect of the El Chichon aerosol layer could not be separated from the strong reflected-shortwave and emitted-longwave perturbations issuing from the El Nino/Southern Oscillation event of 1982-83.

Ardanuy, P. E.↗
NASA NTRS2016

The Formation of Nucleobases from the Irradiation of Purine in Astophysical Ices and Comparisons with Meteorites.

N-heterocycles have been identified in meteorites and their extraterrestrial origins are suggested by isotopic ratio measurements. Although small N- heterocycles have not been detected in the interstellar medium (ISM), recent experiments in our lab have shown that the irradiation of the aromatic molecules like benzene (C6H6) and naphthalene (C10H8) in mixed molecular ices leads to the formation of O- and N-heterocyclic molecules. Among the class of N-heterocycles are the nucleobases, which are of astrobiological interest because they are the information bearing units of DNA and RNA. Nucleobases have been detected in meteorites [3-5], with isotopic signatures that are also consistent with an extraterrestrial origin. Three of the biologically relevant nucleobases (uracil, cytosine, and guanine) have a pyrimidine core structure while the remaining two (adenine and guanine) possess a purine core. Previous experiments in our lab have demonstrated that all of the bio-logical nucleobases (and numerous other molecules) with a pyrimidine core structure can be produced by irradiating pyrimidine in mixed molecular ices of several compositions [6-8]. In this work, we study the formation of purine-based molecules, including the nucleobases adenine, and guanine, from the ultraviolet (UV) irradiation of purine in ices consisting mixtures of H2O and NH3 at low temperature. The experiments are designed to simulate the astrophysical conditions under which these species may be formed in dense molecular clouds, protoplanetary disks, or on the surfaces of icy bodies in planetary systems.

Astrochemistry↗
NASA NTRS2010

Comparisons of the Flare Irradiance Spectral Model (FISM) to Preliminary SDO EVE Data

The Solar Dynamics Observatory (SDO) launched February 11,2010 from Kennedy Space Center and started normal science operations in April 2010. One of the instruments onboard SDO, the EUV Variability Experiment (EVE), will measure the solar EUV irradiance from 0.1-105 nm with 0.1 nm spectral resolution as well as a measure of the broad-band Lyman-Alpha emission (121.6 nm), all with less than 10 percent uncertainties. One of the biggest improvements of EVE over its predecessors is its ability to continuously measure the complete spectrum every 10 seconds, 24 hours a day, 7 days a week. This temporal coverage and cadence will greatly enhance the knowledge of the solar EUV variations during solar flares. This paper will present a comparison of the Flare Irradiance Spectral Model (FISM), which can produce an estimated EUV spectrum at 10 seconds temporal resolution, to the preliminary results from SDO EVE. The discussion will focus on the short-term EUV flare variations and evolution.

Chamberlin, Phillip↗
NASA NTRS2010

Flare Comparisons of the Flare Irradiance Spectral Model (FISM) to Preliminary SDO EVE Data

The Solar Dynamics Observatory (SDO) launched February 11, 2010 from Kennedy Space Center and started normal science operations in April 2010. One of the instruments onboard SDO, the EUV Variability- Experiment (EVE), will measure the solar EUV irradiance from 0.1-105 nm with 0.1 nm spectral resolution as well as a measure of the broad-band Lyman-Alpha emission (121.0 rim), all with less than 10 percent uncertainties. One of the biggest improvements of EVE over its predecessors is its ability to continuously measure the complete spectrum ever y 10 seconds, 24 hours a day, 7 days a week. This temporal coverage and cadence will greatly enhance the knowledge of the solar EUV variations during solar flares. This paper will present a comparison of the Flare Irradiance Spectral Model (FISM), which can produce an estimated EUV spectrum at 10 seconds temporal resolution, to the preliminary flare observation results from SDO EVE. The discussion will focus on the short-term EUV flare variations and evolution.

Chamberlon, Phillip C.↗
NASA NTRS1986

The El Chichon stratospheric aerosol layer as observed by the Nimbus-7 ERB experiment - 1982-1985

Nimbus-7 wide-FOV irradiance data collected from 1981-1985 are used to evaluate the effects of the El Chichon eruptions of 1982 on the earth radiation budget. The north polar region displayed a maximum response of 20 percent in the winter of 1982-1983, with the variation being most apparent in the near-IR 2.8 micron and 0.2-3.8 microns shortwave bands. The data indicate that the particle size distribution was constant for a year after eruptions.

Groveman, B. S.↗
NASA NTRS1988

Solar luminosity variations in solar cycle 21

Long-term variations in the solar total irradiance found in the ACRIM I experiment on the SMM satellite have revealed a downward trend during the declining phase of solar cycle 21 of the sunspot cycle, a flat period between mid-1095 and mid-1987, and an upturn in late 1987 which suggests a direct correlation of luminosity and solar active region population. If the upturn continues into the activity maximum of solar cycle 22, a relation between solar activity and luminosity of possible climatological significance could be ascertained. The best-fit relationship for the variation of total irradiance S with sunspot number Rz and 10-cm flux F(10) are S = 1366.82 + 7.71 x 10 to the -3rd Rz and S = 1366.27 + 8.98 x 10 to the -3rd F(10)(W/sq m). These findings could be used to approximate total irradiance variations over the periods for which these indices have been compiled.

Willson, Richard C.↗