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At least 181 records · Page 10

All sources NASA NTRS DOE OSTI

NASA NTRS2002

Visions of our Planet's Atmosphere, Land and Oceans: NASA/NOAA Electronic Theater 2002

The NASA/NOAA Electronic Theater presents Earth science observations and visualizations in a historical perspective. Fly in from outer space to the 2002 Winter Olympic Stadium Site of the Olympic Opening and Closing Ceremonies in Salt Lake City. Fly in and through Olympic Alpine Venues using 1 m IKONOS "Spy Satellite" data. Go back to the early weather satellite images from the 1960s and see them contrasted with the latest US and international global satellite weather movies including hurricanes and "tornadoes". See the latest visualizations of spectacular images from NASA/NOAA remote sensing missions like Terra, GOES, TRMM, SeaWiFS, Landsat 7 including new 1 - min GOES rapid scan image sequences of Nov 9th 2001 Midwest tornadic thunderstorms and have them explained. See how High-Definition Television (HDTV) is revolutionizing the way we communicate science. (In cooperation with the American Museum of Natural History in NYC) See dust storms in Africa and smoke plumes from fires in Mexico. See visualizations featured on the covers of Newsweek, TIME, National Geographic, Popular Science and on National and International Network TV. New computer software tools allow us to roam and zoom through massive global images e.g. Landsat tours of the US, and Africa, showing desert and mountain geology as well as seasonal changes in vegetation. See animations of the polar ice packs and the motion of gigantic Antarctic Icebergs from SeaWinds. data. Spectacular new visualizations of the global atmosphere and oceans are shown. See vortexes and currents in the global oceans that bring up the nutrients to feed tiny algae and draw the fish, whales and fisherman. See the how the ocean blooms in response to these currents and El Nino/La Nina climate changes. See the city lights, fishing fleets, gas flares and bio-mass burning of the Earth at night observed by the "night-vision" DMSP military satellite.

Haser, Fritz↗

DOE OSTI2022

Short Chain Fatty Acids and Bacterial Taxa Associated with Reduced Salmonella enterica serovar I 4,[5],12:i:- Shedding in Swine Fed a Diet Supplemented with Resistant Potato Starch

Salmonella enterica serovar I 4,[5],12:i:- is a foodborne pathogen of concern because many isolates are multidrug-resistant (resistant to ≥3 antimicrobial classes) and metal tolerant. In this study, three in-feed additives were individually tested for their ability to reduce Salmonella I 4,[5],12:i:- shedding in swine: resistant potato starch (RPS), high amylose corn starch, and a fatty acid blend, compared with a standard control diet over 21 days. Only RPS-fed pigs exhibited a reduction in Salmonella fecal shedding, different bacterial community compositions, and different cecal short chain fatty acid (SCFA) profiles relative to control animals. Within the RPS treatment group, pigs shedding the least Salmonella tended to have greater cecal concentrations of butyrate, valerate, caproate, and succinate. Additionally, among RPS-fed pigs, several bacterial taxa (Prevotella_7, Olsenella, and Bifidobacterium, and others) exhibited negative relationships between their abundances of and the amount of Salmonella in the feces of their hosts. Many of these same taxa also had significant positive associations with cecal concentrations of butyrate, valerate, caproate, even though they are not known to produce these SCFAs. Together, these data suggest the RPS-associated reduction in Salmonella shedding may be dependent on the establishment of bacterial cross feeding interactions that result in the production of certain SCFAs. However, directly feeding a fatty acid mix did not replicate the effect. RPS supplementation could be an effective means to reduce multidrug-resistant (MDR) S. enterica serovar I 4,[5],12:i:- in swine, provided appropriate bacterial communities are present in the gut.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2005

NASA's Earth Observations of the Global Environment

A birds eye view of the Earth from afar and up close reveals the power and magnificence of the Earth and juxtaposes the simultaneous impacts and powerlessness of humankind. The NASA Electronic Theater presents Earth science observations and visualizations in an historical perspective. Fly in from outer space to Africa and Cape Town. See the latest spectacular images from NASA & NOAA remote sensing missions like Meteosat, TRMM, Landsat 7, and Terra, which will be visualized and explained in the context of global change. See visualizations of global data sets currently available from Earth orbiting satellites, including the Earth at night with its city lights, aerosols from biomass burning in the Middle East and Africa, and retreat of the glaciers on Mt. Kilimanjaro. See the dynamics of vegetation growth and decay over Africa over 17 years. New visualization tools allow us to roam & zoom through massive global mosaic images including Landsat and Terra tours of Africa and South America, showing land use and land cover change from Bolivian highlands. Spectacular new visualizations of the global atmosphere & oceans are shown. See massive dust storms sweeping across Africa and across the Atlantic to the Caribbean and Amazon basin. See ocean vortexes and currents that bring up the nutrients to feed tiny phytoplankton and draw the fish, pant whales and fisher- man. See how the ocean blooms in response to these currents and El Nino/La Nifia. We will illustrate these and other topics with a dynamic theater-style presentation, along with animations of satellite launch deployments and orbital mapping to highlight aspects of Earth observations from space.

King, Michael D.↗

NASA NTRS2003

Body water handling in response to hypertonic-saline induced diuresis in fasting northern elephant seal pups (Mirounga angustirostris)

During natural fasting conditions in postweaned northern elephant seal (NES) (Mirounga angustirostris) pups, urinary water loss is minimized and percent total body water (TBW) is maintained constant. However, following infusion of hypertonic saline, glomerular filtration rate (GFR) and urine output increased in fasting pups. Therefore, we quantified the magnitude of the hypernatremia-induced diuresis relative to the animal's total body water (TBW) pool and the percentage of filtered water reabsorbed. Following a 24 h control period, naturally fasting NES pups (n=7) were infused (4 ml min(-1)) with hypertonic saline (16.7%) at a dose of 3 mmol NaCl kg(-1) body mass. Total body water was estimated prior to infusion by tritium dilution, GFR was estimated by standard creatinine clearance, and urine output (V) was measured for 24 h during the control and post infusion periods. Percentage of filtered water reabsorbed was calculated as (1-(V/GFR))x100. Twenty-four hours following the infusion, GFR (control: 69+/-12 ml min(-1) and post-infusion: 118+/-19 ml min(-1); mean+/-S.E.) increased 77+/-28% above control and the percentage of filtered water reabsorbed was decreased 0.4+/-0.1%. The increase in urine output (control: 218+/-47 ml d(-1) and post-infusion: 883+/-92 ml d(-1)) accounted for 1.7+/-0.2% of the pups' TBW. The hypernatremia-induced diuresis was accompanied by the loss of body water indicating the lack of water retention. Although the 77% increase in GFR was only associated with a 0.4% decrease in the percentage of filtered water reabsorbed, this decrease was significant enough to result in a 4-fold increase in urine output. Despite the observed diuresis, fasting NES pups appear to possess an efficient water recycling mechanism requiring only a small percentage of body water to excrete an excess salt load. This water recycling mechanism may allow pups to avoid negative perturbations in body water as they initiate feeding in a marine environment following the fast.

Non-NASA Center↗

DOE OSTI2020

High Rate of Non-Human Feeding by Aedes aegypti Reduces Zika Virus Transmission in South Texas

Mosquito-borne viruses are emerging or re-emerging globally, afflicting millions of people around the world. Aedes aegypti, the yellow fever mosquito, is the principal vector of dengue, Zika, and chikungunya viruses, and has well-established populations across tropical and subtropical urban areas of the Americas, including the southern United States. While intense arboviral epidemics have occurred in Mexico and further south in the Americas, local transmission in the United States has been minimal. Here, we study Ae. aegypti and Culex quinquefasciatus host feeding patterns and vertebrate host communities in residential environments of South Texas to identify host-utilization relative to availability. Only 31% of Ae. aegypti blood meals were derived from humans, while 50% were from dogs and 19% from other wild and domestic animals. In Cx. quinquefasciatus, 67% of blood meals were derived from chicken, 22% came from dogs, 9% from various wild avian species, and 2% from other mammals including one human, one cat, and one pig. We developed a model for the reproductive number, R0, for Zika virus (ZIKV) in South Texas relative to northern Mexico using human disease data from Tamaulipas, Mexico. We show that ZIKV R0 in South Texas communities could be greater than one if the risk of human exposure to Ae. aegypti bites in these communities is at least 60% that of Northern Mexico communities. The high utilization of non-human vertebrates and low risk of human exposure in South Texas diminishes the outbreak potential for human-amplified urban arboviruses transmitted by Ae. aegypti.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1972

The case for long range chemoreceptive piloting in Chelonia

The reproductive ecology and migration habits of Chelonia are investigated. Efforts were made to determine if the turtle navigates by chemoreception and if sensory responses of the migrating animals could be electronically tracked through telemetry. Efforts were also made to: (1) explain why certain small islands or restricted areas of mainland shore are chosen by Chelonia as nesting grounds, even when located a thousand miles or more from the year round feeding grounds of the population; (2) identify guidance mechanisms used by migrants in their periodic open ocean travels; and (3) account for the so called lost year - the virtually complete disappearance of young sea turtles during their first year of life. It was suggested that turtle migration is aided by an olfactory mechanism, sun compass, and ocean currents. The tracking experiment was unsuccessful; the equipment was lost or damaged and stopped functioning after about two hours.

Carr, A. F., Jr.↗

NASA NTRS1977

Carry-on Shuttle payloads, or how to 'CON' the system

The three types of life-science payload carried by the Space Shuttle are briefly discussed. These are the carry-on (CON), the minilab, and the dedicated life-science laboratory. The latter two payloads relate to experiments with animals, plants, and other biological forms. The CON payload is a package weighing up to 200 lb (up to 5 cu ft in size) which, without requiring a crew interface nor direct connection with the Shuttle, will provide such support as power, cooling, feeding, etc. The configuration and principal characteristics of each type of payload are presented.

Winter, D. L.↗

NASA NTRS2002

A Perspective of Our Planet's Atmosphere, Land, and Oceans: A View from Space

A birds eye view of the Earth from afar and up close reveals the power and magnificence of the Earth and juxtaposes the simultaneous impacts and powerlessness of humankind. The NASA Electronic Theater presents Earth science observations and visualizations in an historical perspective. Fly in from outer space to South America with its Andes Mountains and the glaciers of Patagonia, ending up close and personal in Buenos Aires. See the latest spectacular images from NASA & NOAA remote sensing missions like GOES, TRMM, Landsat 7, QuikScat, and Terra, which will be visualized and explained in the context of global change. See visualizations of global data sets currently available from Earth orbiting satellites, including the Earth at night with its city lights, aerosols from biomass burning in South America and Africa, and global cloud properties. See the dynamics of vegetation growth and decay over South America over 17 years, and its contrast to the North American and Africa continents. New visualization tools allow us to roam & zoom through massive global mosaic images from the Himalayas to the dynamics of the Pacific Ocean that affect the climate of South and North America. New visualization tools allow us to roam & zoom through massive global mosaic images including Landsat and Terra tours of South America and Africa showing land use and land cover change from Patagonia to the Amazon Basin, including the Andes Mountains, the Pantanal, and the Bolivian highlands. Landsat flyins to Rio Di Janeiro and Buenos Aires will be shows to emphasize the capabilities of new satellite technology to visualize our natural environment. Spectacular new visualizations of the global atmosphere & oceans are shown. See massive dust storms sweeping across Africa and across the Atlantic to the Caribbean and Amazon basin. See ocean vortexes and currents that bring up the nutrients to feed tiny phytoplankton and draw the fish, giant whales and fisherman. See how the ocean blooms in response to these currents and El Nino/La Nina climate changes. We will illustrate these and other topics with a dynamic theater-style presentation, along with animations of satellite launch deployments and orbital mapping to highlight aspects of Earth observations from space.

King, Michael D.↗

DOE OSTI2021

Recent advancements in the genetic engineering of microalgae

The development of more sustainable food, feed, and bio-products is critical to mitigating the environmental stresses facing our world today. Algae, which includes seaweeds, eukaryotic microalgae, and cyanobacteria, are a promising platform to achieving this, as they have low energy and space requirements, are safe for human and animal consumption, and can be manipulated to produce a diversity of valuable bioproducts. This review focuses on microalgae, both eukaryotic and cyanobacteria. In the past, addressing the major challenges of bringing microalgal production systems to an economically viable scale only had a relatively small genetic toolset to work with, in comparison to other microbial systems such as bacteria and yeast. Expanding the molecular tools available for genetic engineering of microalgae will lead to higher product yields, and accelerate the development of new microalgal bioproducts for commercial applications, thereby supporting the shift towards more environmentally friendly products. In this review, we highlight significant advances from recent years on the design of microalgal expression vectors, discovery of genetic regulatory elements (promoters and transcription factors), optimization of transformation methods, and development of new strain improvement techniques, all aimed at advancing microalgae to become a more efficient biomanufacturing platform. We then discuss how these tools have been applied to improving recombinant protein production, and to enhance metabolic pathway engineering.

09 BIOMASS FUELS↗

NASA NTRS2002

A Perspective of Our Planet's Atmosphere, Land, and Oceans: A View from Space

King, Michael D.↗

NASA NTRS2001

Visions of Our Planet's Atmosphere, Land and Oceans Electronic-Theater 2001

The NASA/NOAA/AMS Electronic Theater presents Earth science observations and visualizations in a historical perspective. Fly in from outer space to Fredericton New Brunswick. Drop in on the Kennedy Space Center and Park City Utah, site of the 2002 Olympics using 1 m IKONOS "Spy Satellite" data. Go back to the early weather satellite images from the 1960s and see them contrasted with the latest US and International global satellite weather movies including hurricanes & tornadoes. See the latest spectacular images from NASA/NOAA and Canadian remote sensing missions like Terra GOES, TRMM, SeaWiFS, Landsat 7, and Radarsat that are visualized & explained. See how High Definition Television (HDTV) is revolutionizing the way we communicate science in cooperation with the American Museum of Natural History in NYC. See dust storms in Africa and smoke plumes from fires in Mexico. See visualizations featured on Newsweek, TIME, National Geographic, Popular Science covers & National & International Network TV. New visualization tools allow us to roam & zoom through massive global images eg Landsat tours of the US, Africa, & New Zealand showing desert and mountain geology as well as seasonal changes in vegetation. See animations of the polar ice packs and the motion of gigantic Antarctic Icebergs from SeaWinds data. Spectacular new visualizations of the global atmosphere & oceans are shown. See massive dust storms sweeping across Africa. See vortexes and currents in the global oceans that bring up the nutrients to feed tiny plankton and draw the fish, whales and fisherman. See the how the ocean blooms in response to these currents and El Nino/La Nina climate changes. The demonstration is interactively driven by a SGI Onyx II Graphics Supercomputer with four CPUs, 8 Gigabytes of RAM and Terabyte of disk. With multiple projectors on a giant screen. See the city lights, fishing fleets, gas flares and bio-mass burning of the Earth at night observed by the "night-vision" DMSP military satellite.

Hasler, A. F.↗

NASA NTRS2001

Electronic-Theater 2001: Visions of Our Planet's Atmosphere, Land and Oceans

The NASA/NOAA/AMS Electronic Theater presents Earth science observations and visualizations in a historical perspective. Fly in from outer space to Wisconsin, Madison and the Monona Terrace Center. Drop in on the Kennedy Space Center and Park City Utah, site of the 2002 Olympics using I m IKONOS "Spy Satellite" data. Go back to the early weather satellite images from the 1960s pioneered by UW. Scientists and see them contrasted with the latest US and International global satellite weather movies including hurricanes & tornadoes. See the latest spectacular images from NASA/NOAA remote sensing missions like Terra GOES, TRMM, SeaWiFS, Landsat 7 that are visualized & explained. See how High Definition Television (HDTV) is revolutionizing the way we communicate science in cooperation with the American Museum of Natural History in NYC. See dust storms in Africa and smoke plumes from fires in Mexico. See visualizations featured on Newsweek, TIME, National Geographic, Popular Science covers & National & International Network TV. New visualization tools allow us to roam & zoom through massive global images eg Landsat tours of the US, Africa, & New Zealand showing desert and mountain geology as well as seasonal changes in vegetation. See animations of the polar ice packs and the motion of gigantic Antarctic Icebergs from SeaWinds data. Spectacular new visualizations of the global atmosphere & oceans are shown. See massive dust storms sweeping across Africa. See vortices and currents in the global oceans that bring up the nutrients to feed tiny plankton and draw the fish, whales and fisherman. See the how the ocean blooms in response to these currents and El Nina/La Nina climate changes. The demonstration is interactively driven by a SGI Onyx 11 Graphics Supercomputer with four CPUs, 8 Gigabytes of RAM and Terabyte of disk. With five projectors on a giant IMAX sized 18 x 72 ft screen. See the city lights, fishing fleets, gas flares and bio-mass burning of the Earth at night observed by the "nightvision" DMSP military satellite.

Hasler, Authur↗

NASA NTRS1987

Environmental control and life support systems analysis for a Space Station life sciences animal experiment

NASA's Langley and Goddard facilities have evaluated the effects of animal science experiments on the Space Station's Environmental Control and Life Support System (ECLSS) by means of computer-aided analysis, assuming an animal colony consisting of 96 rodents and eight squirrel monkeys. Thirteen ECLSS options were established for the reclamation of metabolic oxygen and waste water. Minimum cost and weight impacts on the ECLSS are found to accrue to the system's operation in off-nominal mode, using electrochemical CO2 removal and a static feed electrolyzer for O2 generation.

So, Kenneth T.↗

NASA NTRS2000

Circadian Behavioral Study: LED vs Cool White Fluorescent - 0.1, 1, 10, 40, 80 lux

Currently, the light source most commonly used in animal habitat lighting is cool white fluorescent (CWF) light. It was the objective of this study to evaluate a novel LED light source for use in animal habitat lighting by comparing its effectiveness to CWF light in producing and maintaining a normal circadian entrainment. The LED and CWF lights had similar spectral power distributions. Sprague-Dawley rats (175-350 g) were kept individually in metabolic cages, under a strict lighting control: 4 days of acclimation at 12:12 LD, 14 days of 12:12 LD, 14 days of 24:0 LD (free-run), and finally 12:12 LD. Food and water were provided ad libitum. Three behavioral parameters were monitored continuously: gross locomotor activity, drinking, and feeding. Combined mean free run periods (tau) were (mean +/- SEM): 24.6 +/- 0.1 and 24.7 +/- 0.2 at 0.1 lux, 25.5 +/- 0.1 and 25.7 +/- 0.1 at 1.0 lux, 25.3 +/- 0.2 and 25.4 +/- 0.2 at 10 lux, 25.8 +/- 0.1 and 25.9 +/- 0.1 at 40 lux, and 25.9 +/- 0.1 and 25.9 +/- 0.1 at 80 lux, CWF and LED respectively. ANOVA found a significant effect (p < 0.05) due to light level, but no difference in tau between rats exposed to constant CWF light and rats exposed to constant LED light. This study has shown that LED light can produce the same entrainment pattern as a conventional CWT light at similar intensities (0.1, 1, 10, 40, and 80 lux). LED light sources may be a suitable replacement for conventional light sources used in animal habitat lighting while providing many mechanical and economical advantages.

Holley, Daniel C.↗

NASA NTRS2003

Is the food-entrainable circadian oscillator in the digestive system?

Food-anticipatory activity (FAA) is the increase in locomotion and core body temperature that precedes a daily scheduled meal. It is driven by a circadian oscillator but is independent of the suprachiasmatic nuclei. Recent results that reveal meal-entrained clock gene expression in rat and mouse peripheral organs raise the intriguing possibility that the digestive system is the site of the feeding-entrained oscillator (FEO) that underlies FAA. We tested this possibility by comparing FAA and Per1 rhythmicity in the digestive system of the Per1-luciferase transgenic rat. First, rats were entrained to daytime restricted feeding (RF, 10 days), then fed ad libitum (AL, 10 days), then food deprived (FD, 2 days). As expected FAA was evident during RF and disappeared during subsequent AL feeding, but returned at the correct phase during deprivation. The phase of Per1 in liver, stomach and colon shifted from a nocturnal to a diurnal peak during RF, but shifted back to nocturnal phase during the subsequent AL and remained nocturnal during food deprivation periods. Second, rats were entrained to two daily meals at zeitgeber time (ZT) 0400 and ZT 1600. FAA to both meals emerged after about 10days of dual RF. However, all tissues studied (all five liver lobes, esophagus, antral stomach, body of stomach, colon) showed entrainment consistent with only the night-time meal. These two results are inconsistent with the hypothesis that FAA arises as an output of rhythms in the gastrointestinal (GI) system. The results also highlight an interesting diversity among peripheral oscillators in their ability to entrain to meals and the direction of the phase shift after RF ends.

Non-NASA Center↗

NASA NTRS1997

Baroreflex regulation of blood pressure during dynamic exercise

From the work of Potts et al. Papelier et al. and Shi et al. it is readily apparent that the arterial (aortic and carotid) baroreflexes are reset to function at the prevailing ABP of exercise. The blood pressure of exercise is the result of the hemodynamic (cardiac output and TPR) responses, which appear to be regulated by two redundant neural control systems, "Central Command" and the "exercise pressor reflex". Central Command is a feed-forward neural control system that operates in parallel with the neural regulation of the locomotor system and appears to establish the hemodynamic response to exercise. Within the central nervous system it appears that the HLR may be the operational site for Central Command. Specific neural sites within the HLR have been demonstrated in animals to be active during exercise. With the advent of positron emission tomography (PET) and single-photon emission computed tomography (SPECT), the anatomical areas of the human brain related to Central Command are being mapped. It also appears that the Nucleus Tractus Solitarius and the ventrolateral medulla may serve as an integrating site as they receive neural information from the working muscles via the group III/IV muscle afferents as well as from higher brain centers. This anatomical site within the CNS is now the focus of many investigations in which arterial baroreflex function, Central Command and the "exercise pressor reflex" appear to demonstrate inhibitory or facilitatory interaction. The concept of whether Central Command is the prime mover in the resetting of the arterial baroreceptors to function at the exercising ABP or whether the resetting is an integration of the "exercise pressor reflex" information with that of Central Command is now under intense investigation. However, it would be justified to conclude, from the data of Bevegard and Shepherd, Dicarlo and Bishop, Potts et al., and Papelier et al. that the act of exercise results in the resetting of the arterial baroreflex. In addition, if, as we have proposed, the cardiopulmonary baroreceptors primarily monitors and reflexly regulates cardiac filling volume, it would seem from the data of Mack et al. and Potts et al. that the cardiopulmonary baroreceptor is also reset at the beginning of exercise. Therefore, investigations of the neural mechanisms of regulation involving Central Command and cardiopulmonary afferents, similar to those being undertaken for the arterial baroreflex, need to be established.

NASA Discipline Cardiopulmonary↗

NASA NTRS2017

Behavioral Adaptations of Female Mice on the International Space Station

Adult female mice were sent to the International Space Station (ISS) as part of an early life science mission utilizing NASA's Rodent Habitat. Its primary purpose was to provide further insight into the influence of a microgravity environment on various aspects of mammalian physiology and well-being as part of an ongoing program of research aimed ultimately at understanding and ameliorating the deleterious influences of space on the human body. The present study took advantage of video collected from fixed, in-flight cameras within the habitat itself, to assess behavioral adaptations observed among in-flight mice aboard the ISS and differences in behavior with respect to a control group on the ground. Data collection consisted of several behavioral measures recorded by a trained observer with the assistance of interactive behavior analysis software. Specific behavioral measures included frequencies of conspecific interactionsociability, time spent feeding and conducting hygienic behavior, and relative durations of thigmotactic behavior, which is commonly used as an index of anxiety. Data were used to test tentative hypotheses that such behaviors differ significantly across mice under microgravity versus 1g conditions, and the assumption that the novel experience of microgravity itself may represent an initially anxiogenic stimulus which an animal will eventually acclimate to, perhaps through habituation.

Behavior in space↗

NASA NTRS2017

Behavioral Adaptations of Female Mice on the International Space Station

rodent behavior↗