DOE OSTI · code-164643
Yeast Transformation on Hamilton Vantage (YT Vantage) v1
Abstract
Our software program is designed for the Hamilton Vantage liquid handling robot, automating the Build step in the Design-Build-Test-Learn (DBTL) cycle for Saccharomyces cerevisiae. This program minimizes human intervention, enabling rapid identification of pathway bottlenecks and genes that enhance verazine production. The program takes competent yeast and plasmid DNA as input and generates an output library of engineered strains compatible with automated colony picking, high-throughput culturing, and chemical extraction for downstream LC-MS analysis. A user-friendly interface, developed using the Hamilton Method Editor software, allows for on-demand parameter customization. By automating this process, our program streamlines the construction of Saccharomyces cerevisiae, reducing manual labor and increasing efficiency. While the manual process is well-documented, integration with robotic automation is less common, making our program a valuable tool for researchers. With this software, we achieved 2-5 fold increases in verazine production, demonstrating its potential to accelerate research in this field.
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Louie, Randy [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Tan, Stephen [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Tavares Astolfi, Maria Clara [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley (UCB)], Yoder, Sam [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]. 2025-09-09. Yeast Transformation on Hamilton Vantage (YT Vantage) v1. https://doi.org/10.11578/dc.20250919.5
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