DOE OSTI · 3023653
An activity-based probe library for identifying promiscuous amide hydrolases
Abstract
A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to putative hydrolases. This approach prioritizes candidate hydrolases for further experimental validation with potential applications in the environment, biomanufacturing, and medicine.
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Kombala Nanayakkara Thambiliya, Chathuri Jeewanthi [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000238478769), Kroll, Jared O. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000190660579), Webber, Lucas C. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Callister, Stephen J. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Sadler, Natalie C. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Bredeweg, Erin L. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000178278342), Medrano, Irlanda N. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0009000591174069), Brandvold, Kristoffer R. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:000000015970879X), Krishnamoorthy, Sankarganesh [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000162648791). 2025-10-01. An activity-based probe library for identifying promiscuous amide hydrolases. https://doi.org/10.1039/d5cc04162g
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