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DOE OSTI · 3013015

Pseudomonas putida membrane vesicles

Wilkes, Rebecca A.·Miller, Tarryn E.·Waldbauer, Jacob·Zhou, Nanqing·Zhang, Lichun·DiBiase, Beth N.·Kamat, Neha P.·Aristilde, Ludmilla·Beckham, Gregg T.·Werner, Allison Z.
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Abstract

Cellular and membrane vesicle proteomes from Pseudomonas putida KT2440 and engineered derivative strains. Proteomic quantification by diDO-IPTL (Waldbauer et al. 2017 Analytical Chemistry).

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hypervesiculationmembraneouter membrane vesiclesproteinproteomicssecretionvesicles

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Wilkes, Rebecca A., Miller, Tarryn E., Waldbauer, Jacob, Zhou, Nanqing, Zhang, Lichun, DiBiase, Beth N., Kamat, Neha P., Aristilde, Ludmilla, Beckham, Gregg T., Werner, Allison Z.. 2026-01-14. Pseudomonas putida membrane vesicles. https://doi.org/10.25983/cbi%2F3013015

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Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida -putative knockouts table

Table S1, putative gene knockout targets in P. putida KT2440 to enhance vesiculation; Table S2, protein sequence identity of OmpA from E. coli K12 to P. putida KT2440 genes; Table S3, strains utilized in this study and corresponding construction details; Table S4, oligonucleotides utilized in this study; Table S5, plasmids utilized in this study; Table S6, sequences for mNeonGreen, tags, and codon-optimized genes; Figure S1, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 1B; Figure S2, OD600 measurements of extracted MVs from KT2440 and knockout strains; Figure S3, particle count per gCDW for WT, ΔPP_4669, and ΔPP_1502; Figure S4, particle count per gCDW for KT2440 and knockout strains corresponding to data presented in Figure 3C; Figure S5, sizes of MVs corresponding to particle counts in Figure S4; Figure S6, particle count per gCDW for KT2440 grown on 20 mM glucose alone or 20 mM glucose plus 12.5 mM p-coumarate and 12.5 mM ferulate; Figure S7 and Figure S8, principal component analysis of the cellular fractions; Figure S9, heatmap of outer membrane proteins with differential abundance; and Figure S10, mNeonGreen (mNG) fluorescence signal for the cellular fraction and the extracellular fraction

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