Enzyme Engineering for Expanded Product Scope (CRADA Final Report)

Kirchstetter, Thomas; Boville, Christina

doi:10.2172/2570951

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DOE OSTI · 2570951

Enzyme Engineering for Expanded Product Scope (CRADA Final Report)

Kirchstetter, Thomas·Boville, Christina

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Abstract

As part of the Cyclotron Road program, Aralez Inc. investigated methods for producing chemicals sustainably, with a particular focus on engineering enzymes to make novel chemical products or accept new substrates that aren’t found in nature. We used directed evolution and other protein engineering techniques to create enzymes that perform abiological chemistry or make chemical products through a non-natural synthetic route. This work was an important step towards increasing the scope of compounds that can be manufactured in a sustainable fashion, which in turn will alleviate the high energy consumption, costly emissions, and waste that are hallmarks of current methods in chemical manufacturing.

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Kirchstetter, Thomas, Boville, Christina. 2022-01-16. Enzyme Engineering for Expanded Product Scope (CRADA Final Report). https://doi.org/10.2172/2570951

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